CN1482443A - Plant root excretion continuous collection method and apparatus - Google Patents
Plant root excretion continuous collection method and apparatus Download PDFInfo
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- CN1482443A CN1482443A CNA021382581A CN02138258A CN1482443A CN 1482443 A CN1482443 A CN 1482443A CN A021382581 A CNA021382581 A CN A021382581A CN 02138258 A CN02138258 A CN 02138258A CN 1482443 A CN1482443 A CN 1482443A
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Abstract
A plant root excretion continuous collection method and apparatus, wherein the plant is placed in the cultivation dish equipped with quartz sand, the bottom opening of the cultivation dish is connected to a glass bottle containing resin through pipe lines, an opening is arranged at the bottom of the glass bottle, then connected to the cultivation dish through pipe lines, forming a circulated system, adding different solvents into the circulating pipes, for the three dissimilar collected root secretion, i.e., neutral, acidic or basic, making the root secretion absorbed on the surface of the resin when flowing through the resin along with the solvent of the circulatory flow, after a specific period of time, perform elution, compression and filtration to the root secretion, finally conduct measuring.
Description
One, technical field
The present invention relates to the collection of root exudations, especially a kind of root exudations continuous collection method and device thereof, dependent territory earth, microorganism and plant science field.
Two, background technology
Except cell that comes off and the mucus that is produced, plant roots is also secreted miscellaneous solable matter, as molecular weight carbohydrate not of uniform size, organic acid, amino acid, fatty acid, steroid class, enzyme, auximone etc., wherein be mainly carbohydrate, organic acid and amino acid.Root secretion because of floristics, physiological factor (as the age, nutrition condition) and abiotic condition (as temperature, soil texture, aeration and moisture) and widely different, also with activation of microorganism (quantity consumed), it is relevant to enter the quantity in the root and the degree of injury of root etc. after secreting out from root again, if collect then relevant with the position of microscale sampler in soil.Root secretion is easily utilized by Institute of Micro-biology, is commonly referred to be rhizosphere microorganism quantity and the active one of the main reasons that increases.Total root exudates accounts for a big chunk of photosynthesis of plant product, and the amount up to 70-80% is transferred to root sometimes, but is generally 12-40%.Root secretion is the important component part that material moves and regulates and control in the rhizosphere microecosystem, therefore studies root secretion and has great importance.
Yet, we are extremely limited to the understanding of rhizosphere chemistry now, main because the researcher often faces problem aspect following three when the research root rhizosphere secreta: the common content of the secretion that collect is extremely low, thereby needs a large amount of plants and prolongation acquisition time to obtain the secretion of q.s; The collection of sample and processing are very loaded down with trivial details, and the compound of some less stables will sex change; Growth matrix, container, nutrient solution, water, extractant etc. will cause serious pollution to sample when the conventional solvent extraction was collected micro-secretion.Although the direct extracting soil liquid of rhizosphere soil solution sampler energy original position, but its pvc pipe contains 30% plastifier and a spot of stabilizing agent, the sample of trace can enter sample, in addition, the solution of being got often contains a large amount of soil organism, therefore also can cause bigger pollution and interference to sample.In addition, soil is a very complicated heterogeneous system, and the influence factor of soil solution chemistry is not also understood fully, has spatial and temporal variation in the soil in addition, this has increased many difficulties for the representative soil liquid of collection, thereby just is difficult to collect the solution that can represent real root secretion.
Three, summary of the invention
Collect the shortcoming of root secretion in order to overcome prior art, the present invention proposes a kind of root exudations continuous collection method and device thereof, its technical scheme is as follows:
A kind of root exudations continuous collection method, it is characterized in that plant is placed circulation line, be provided with resin sorbent in this pipeline, it is dissimilar to be neutrality, acidity or three kinds of alkalescence at the root secretion that will collect, add in the circulation line with different solution, make root secretion along with the flow of solution that circulates is adsorbed in resin surface when the resin, one regularly backward, and the root secretion that obtains on the resin is measured through wash-out, after concentrating, filtering.Plant can be placed the Planting utensil that silica sand is housed, the Planting utensil bottom opening is communicated to a vial that resin is housed by the road, the vial bottom opening, return by the road again and pass to Planting utensil, constitute a round-robin system, after 60-72 hour, the root secretion that obtains on the resin is measured through wash-out, after concentrating, filtering.Before collecting continuously, need carry out following preparation work:
1. silica sand: select white silica sand, particle diameter 0.5-1mm, soaked 24 hours, wash silica sand with tap water then with watery hydrochloric acid (2mol/L), solution is neutral (PH=7.0) until the flushing back, sterilize then, dry in the Planting utensil of packing into, and, prevent that silica sand from spilling ware underseal mouth;
2. resin: collectionization is helped to change the neutral uncharged polarity organic radical secretion of compound class, use neutral exchange resin, the resin usefulness methyl alcohol drip washing decon before the vial of packing into; Collect the positively charged acid root secretion of amino acids, use Zeo-karb, be mixed with the solution drip washing decon of acidity (PH=4.0) before the resin bottling with deionized water and watery hydrochloric acid (0.1mol/L); Collect the electronegative alkaline root secretion anion exchange resins of organic acid, be mixed with the solution drip washing decon of alkalescence (PH=8.0) before the resin bottling with deionized water and NaOH (0.1mol/L); With cleansing solution immersion back bottling separately, three types root secretion can be collected respectively or collect simultaneously, when collecting simultaneously, the layering after solution drip washing separately of resin in the vial is placed, isolate with glass wool between each layer, collect simultaneously in two kinds of root secretions, when having the situation of neutral root secretion or three kinds to collect simultaneously, neutral resins should be placed on orlop.The collection process step is as follows:
In the silica sand in the healthy seed immigration Planting utensil that 1. at first the plant seedlings that survives maybe will be sprouted, the communicating pipe of Planting utensil bottom is disconnected, regularly pour into flushing from the Planting utensil top for several times with nutrient solution, after treating that nutrient solution drains off, the Planting utensil bottom is closed communicating pipe, added a certain amount of nutrient solution again and keep the required moisture of plant normal growth;
2. collect neutral root secretion: the vial that the neutral exchange resin that aforementioned processing crosses will be housed inserts connecting pipeline, form circulation line, and in pipeline, insert inflation inlet, start inflator pump, make bubble promote the solution circulation, to adsorb uncharged polarity organic radical secretion on the resin, collect after 60-72 hour, vial is taken off, extract the interior excessive moisture of resin in the post out, wash out root secretion, in 40 ± 1 ℃ of water baths, concentrating under the vacuum decompression condition then with aforementioned neutral cleansing solution, last constant volume filters, and filtrate is preserved until mensuration in refrigerator.
3. collection acidity or alkaline root secretion: on the basis of above-mentioned 1. item, successively with distilled water and deionized water rinsing several, resin cation or the resin anion (R.A.) vial handled will be housed then again, and insert connecting pipeline, following process together 2.;
4. neutral, acid, alkaline root secretion is collected simultaneously: collection process is with 2., and the three layers of resin dealt with separately of just packing in the vial, are measured respectively after concentrating, filtering last layering wash-out.
5. above-mentioned Xian takes off, concentrates and filter process is to use cleansing solution separately to wash out root secretion respectively for several times, each several part root secretion solution is being concentrated into 15mL under the vacuum decompression condition respectively in 40 ± 1 ℃ of water baths, washing is settled to 20mL then, cross the acetate fiber miillpore filter of 0.45um, filtrate is preserved in refrigerator until mensuration, and the flow velocity of regulating circulation solution is 1L/h.
A kind of root exudations continuously trapping system, it is characterized in that being provided with the plant culture ware that silica sand is housed in, its bottom opening, opening part seals with rubber stopper, have through hole on the rubber stopper, insert a communicating pipe, be communicated with and be provided with the glass wool isolation between the mouth of pipe and the silica sand, communicating pipe, to be communicated to the vial that resin is housed after through a valve suitable for reading in the lower end, after the vial sealed rubber plug that has through hole that also is provided with suitable for reading, vial end opening were isolated by glass wool, to pass to Planting utensil suitable for reading through returning communicating pipe again, be provided with inflation inlet in this section pipeline, Planting utensil and vial are light tight wall.Inflation inlet preferably is positioned at the vial centre-height, should discharge air in the vial before the inflation.
The present invention collects with absorption method, and in terms of existing technologies, the collection process time is shorter, and collected root secretion is really representative.
Four, description of drawings
Fig. 1 is a kind of structure of the continuously trapping system among the present invention.
Five, embodiment
As shown in Figure 1, test plant 1 is placed in the silica sand 3 of glass Planting utensil 4, Planting utensil 4 is preferably brown and pastes with aluminium foil 2 bags at outer wall, to reach better light tight effect.Also build with aluminium foil at the top of Planting utensil 4, stretch out for plant but have a hole, and with the good hole of degreasing tampon edge, in case chlorella growth.Have aperture beyond the Great Wall for penetrating glass communicating pipe 8, fill in the glass wool layer 5 that can be provided with between 6 upper surfaces and the silica sand 3 after washing down with tap water after the salt acid soak, in case silica sand leaks down.Communicating pipe 8 is provided with variable valve 7, and end opening is communicated to the vial 10 that resin 11 is housed through rubber stopper 9 mesopores, and appearance also will be used aluminium foil 2 shadings.Vial 10 end openings, upwards crooked through silica gel or Teflon pipe 12 again after isolating with glass wool, return through T shaped inflatable pumping hole 13 and glass tube 14 and to pass to the plant culture ware.A certain amount of glass wool 5 also should have been filled in resin top and bottom in vial, and rubber stopper 6 and 9 appearance preferably are coated with one deck silicone, with the organic principle stripping in antifouling, disturbs collecting effect.
[embodiment 1] provides the plant growth required nutrient with Huo Gelan (Hoagland) nutrient solution of 1/3 intensity, after treating that plant grows into the regular period (about 20 days), do not have silica sand 4 times (each 200ml) in the end glass dish with distilled water towards Xian, use deionized water (18.2M Ω 2cm) Xian 2 times then.The devices such as vial that Amberlite IRC-50 resin cation is housed are loaded onto, connected with the mains, inflator pump charges into continuous bubble, and bubble will make the resin of solution circulation flow in vial, and this resin will adsorb amino acid.Collect after 60-72 hour, the solution in the vial is drained, wash-out concentrates and filters.Derive or measure amino acid without the back of deriving.
[embodiment 2] provide the required nutrient of plant growth of inoculation mycorrhizal fungi with Huo Gelan (Hoagland) nutrient solution of 1/3 intensity, after treating that plant grows into the regular period (about 20 days), do not have silica sand 4 times (each 200ml) in the end glass dish with distilled water towards Xian, use deionized water (18.2M Ω cm) Xian 2 times then.The devices such as vial that Amberlite IRC-50 resin cation is housed are loaded onto, connected with the mains, inflator pump charges into continuous bubble, and bubble will make the resin of solution circulation flow in vial, and this resin will adsorb amino acid.Collect after 60-72 hour, the solution in the vial is drained, wash-out concentrates and filters.Derive or measure amino acid without the back of deriving.
[embodiment 3] provide the plant growth required nutrient with Huo Gelan (Hoagland) nutrient solution of 1/3 intensity, after treating that plant grows into the regular period (about 20 days), do not have silica sand 4 times (each 200ml) in the end glass dish with distilled water towards Xian, use deionized water (18.2M Ω cm) Xian 2 times (each 200ml) then.The devices such as vial that the Dowex-1 resin anion (R.A.) is housed are loaded onto, connected with the mains, inflator pump charges into continuous bubble, and bubble will make the resin of solution circulation flow in vial, and resin will adsorb organic acid.Collect after 60-72 hour, the solution in the vial is drained, wash-out concentrates and filters.Measure organic acid.
[embodiment 4] provide the heavy metal hyperaccumulative plant growth required nutrient with Huo Gelan (Hoagland) nutrient solution of 1/3 intensity, after treating that plant grows into the regular period (about 20 days), do not have silica sand 4 times (each 200ml) in the end glass dish with distilled water towards Xian, use deionized water (18.2M Ω cm) Xian 2 times (each 200ml) then.The devices such as vial that the Dowex-1 resin anion (R.A.) is housed are loaded onto, connected with the mains, inflator pump charges into continuous bubble, and bubble will make the resin of solution circulation flow in vial, and resin will adsorb organic acid.Collect after 60-72 hour, the solution in the vial is drained, wash-out concentrates and filters.Measure hyperaccumulative plant root secretion organic acid.
[embodiment 5] provide the plant growth required nutrient with Huo Gelan (Hoagland) nutrient solution of 1/3 intensity, after treating that plant grows into the regular period (about 20 days), the devices such as vial that the neutral exchange resin of XAD-4 is housed are loaded onto, connect with the mains, inflator pump charges into continuous bubble, bubble will make the resin of solution circulation flow in vial, this resin will adsorb uncharged polarity organic radical secretion.Collect after 60-72 hour, the solution in the vial is drained, wash-out concentrates and filters.Measure polar organic matter.
[embodiment 6] provide the plant growth required nutrient with Huo Gelan (Hoagland) nutrient solution of 1/3 intensity, after treating that plant grows into the regular period (about 20 days), there is not silica sand 4 times (each 200ml) in the end glass dish with distilled water towards Xian, Amberlite IRC50 Zeo-karb will be housed, Dowex-1 anion exchange resins, the devices such as vial of the neutral exchange resin of XAD-4 are loaded onto, connect with the mains, inflator pump charges into continuous bubble, bubble will make the resin of solution circulation flow in vial, and these three layers of different resins are with the root secretion of the different electric charges of adsorption band.Collect after 60-72 hour, the solution in the vial is drained, the layering wash-out concentrates and filters.Measure the root secretion of each layer.
[embodiment 7] Huo Gelan (Hoagland) nutrient solution with 100% provides the plant growth required nutrient, after treating that plant grows into the regular period (about 20 days), the devices such as vial that Amberlite IRC50 Zeo-karb, Dowex-1 anion exchange resins, the neutral exchange resin of XAD-4 are housed are loaded onto, connect with the mains, inflator pump charges into continuous bubble, bubble will make the resin of solution circulation flow in vial, and these three layers of different resins are with the root secretion of the different electric charges of adsorption band.Collect after 60-72 hour, the solution in the vial is drained, the layering wash-out concentrates and filters.Measure the root secretion of each layer.
[embodiment 8] Xiu Yite (Hewitt) nutrient solution with 100% provides the plant growth required nutrient, after treating that plant grows into the regular period (about 20 days), do not have silica sand 4 times (each 200ml) in the end glass dish with distilled water towards Xian, use deionized water (18.2M Ω 2cm) Xian 2 times then.The devices such as vial that Amberlite IRC50 Zeo-karb, Dowex-1 anion exchange resins, the neutral exchange resin of XAD-4 are housed are loaded onto, connect with the mains, inflator pump charges into continuous bubble, bubble will make the resin of solution circulation flow in vial, and these three layers of different resins are with the root secretion of the different electric charges of adsorption band.Collect after 60-72 hour, the solution in the vial is drained, the layering wash-out concentrates and filters.Measure the root secretion of each layer.
[embodiment 9] provide the plant growth required nutrient with body Yi Te (Hewitt) nutrient solution of 1/3 intensity, after treating that plant grows into the regular period (about 20 days), do not have silica sand 4 times (each 200ml) in the end glass dish with distilled water towards Xian, use deionized water (18.2M Ω cm) Xian 2 times then.The devices such as vial that Amberlite IRC50 Zeo-karb, Dowex-1 anion exchange resins, the neutral exchange resin of XAD-4 are housed are loaded onto, connect with the mains, inflator pump charges into continuous bubble, bubble will make the resin of solution circulation flow in vial, and these three layers of different resins are with the root secretion of the different electric charges of adsorption band.Collect after 60-72 hour, the solution in the vial is drained, the layering wash-out concentrates and filters.Measure the root secretion of each layer.
[embodiment 10] provide the plant growth required nutrient with scarce phosphorus Huo Gelan (Hoagland) nutrient solution of 1/3 intensity, after treating that plant grows into the regular period (about 20 days), do not have silica sand 4 times (each 200ml) in the end glass dish with distilled water flushing, use deionized water (18.2M Ω cm) Xian 2 times then.The devices such as vial that Amberlite IRC50 Zeo-karb, Dowex-1 anion exchange resins, the neutral exchange resin of XAD-4 are housed are loaded onto, connect with the mains, inflator pump charges into continuous bubble, bubble will make the resin of solution circulation flow in vial, and these three layers of different resins are with the root secretion of the different electric charges of adsorption band.Collect after 60-72 hour, the solution in the vial is drained, the layering wash-out concentrates and filters.Measure the root secretion of each layer.
[embodiment 11] provide the plant growth required nutrient with rich phosphorus Huo Gelan (Hoagland) nutrient solution of 1/3 intensity, after treating that plant grows into the regular period (about 20 days), do not have silica sand 4 times (each 200ml) in the end glass dish with distilled water flushing, use deionized water (18.2M Ω cm) Xian 2 times then.The devices such as vial that Amberlite IRC50 Zeo-karb, Dowex-1 anion exchange resins, the neutral exchange resin of XAD-4 are housed are loaded onto, connect with the mains, inflator pump charges into continuous bubble, bubble will make the resin of solution circulation flow in vial, and these three layers of different resins are with the root secretion of the different electric charges of adsorption band.Collect after 60-72 hour, the solution in the vial is drained, the layering wash-out concentrates and filters.Measure the root secretion of each layer.
[embodiment 12] provide the plant growth required nutrient with iron deficiency Huo Gelan (Hoagland) nutrient solution of 1/3 intensity, after treating that plant grows into the regular period (about 20 days), do not have silica sand 4 times (each 200ml) in the end glass dish with distilled water towards Xian, use deionized water (18.2M Ω 2cm) Xian 2 times then.The devices such as vial that Amberlite IRC50 Zeo-karb, Dowex-1 anion exchange resins, the neutral exchange resin of XAD-4 are housed are loaded onto, connect with the mains, inflator pump charges into continuous bubble, bubble will make the resin of solution circulation flow in vial, and these three layers of different resins are with the root secretion of the different electric charges of adsorption band.Collect after 60-72 hour, the solution in the vial is drained, the layering wash-out concentrates and filters.Measure the root secretion of each layer.
[embodiment 13] provide the plant growth required nutrient with rich iron Huo Gelan (Hoagland) nutrient solution of 1/3 intensity, after treating that plant grows into the regular period (about 20 days), do not have silica sand 4 times (each 200ml) in the end glass dish with distilled water flushing, use deionized water (18.2M Ω cm) Xian 2 times then.The devices such as vial that Amberlite IRC50 Zeo-karb, Dowex-1 anion exchange resins, the neutral exchange resin of XAD-4 are housed are loaded onto, connect with the mains, inflator pump charges into continuous bubble, bubble will make the resin of solution circulation flow in vial, and these three layers of different resins are with the root secretion of the different electric charges of adsorption band.Collect after 60-72 hour, the solution in the vial is drained, the layering wash-out concentrates and filters.Measure the root secretion of each layer.
Claims (7)
1. root exudations continuous collection method, it is characterized in that plant is placed circulation line, be provided with resin sorbent in this pipeline, it is dissimilar to be neutrality, acidity or three kinds of alkalescence at the root secretion that will collect, add in the circulation line with different solution, make root secretion along with the flow of solution that circulates is adsorbed in resin surface when the resin, one regularly backward, and the root secretion that obtains on the resin is measured through wash-out, after concentrating, filtering.
2. root exudations continuous collection method according to claim 1, it is characterized in that plant is placed the Planting utensil that silica sand is housed, the Planting utensil bottom opening is communicated to a vial that resin is housed by the road, the vial bottom opening, return by the road again and pass to Planting utensil, constitute a round-robin system, after 60-72 hour, the root secretion that obtains on the resin is measured through wash-out, after concentrating, filtering.
3. root exudations continuous collection method according to claim 2, before it is characterized in that collecting continuously, need carry out following preparation work:
1. silica sand: select white silica sand, particle diameter 0.5-1mm, soak with watery hydrochloric acid, wash silica sand with tap water then, solution be neutrality until the flushing back, sterilizes then, dries in the Planting utensil of packing into, and with ware underseal mouth, prevent that silica sand from spilling;
2. resin: collectionization is helped to change the neutral uncharged polarity organic radical secretion of compound class, use neutral exchange resin, the resin usefulness methyl alcohol drip washing decon before the vial of packing into; Collect the positively charged acid root secretion of amino acids, use Zeo-karb, be mixed with acid solution drip washing decon with deionized water and watery hydrochloric acid before the resin bottling; Collect the electronegative alkaline root secretion anion exchange resins of organic acid, be mixed with the solution drip washing decon of alkalescence before the resin bottling with deionized water and NaOH; Soak the back bottling with separately cleansing solution, three types root secretion can be collected respectively or collect simultaneously, and when collecting simultaneously, the layering after solution drip washing separately of the resin in the vial is placed, and isolates with glass wool between each layer.
4. root exudations continuous collection method according to claim 3 is characterized in that collecting simultaneously in two kinds of root secretions, and when having the situation of neutral root secretion or three kinds to collect simultaneously, neutral resins should be placed on orlop.
5. according to claim 2 or 3 or 4 described root exudations continuous collection methods, it is characterized in that the collection process step is as follows:
In the silica sand in the healthy seed immigration Planting utensil that 1. at first the plant seedlings that survives maybe will be sprouted, the communicating pipe of Planting utensil bottom is disconnected, regularly pour into flushing from the Planting utensil top for several times with nutrient solution, after treating that nutrient solution drains off, the Planting utensil bottom is closed communicating pipe, added a certain amount of nutrient solution again and keep the required moisture of plant normal growth;
2. collect neutral root secretion: the vial that the neutral exchange resin that aforementioned processing crosses will be housed inserts connecting pipeline, form circulation line, and in pipeline, insert inflation inlet, start inflator pump, make bubble promote the solution circulation, to adsorb uncharged polarity organic radical secretion on the resin, collect after 60-72 hour, vial is taken off, extract the interior excessive moisture of resin in the post out, wash out root secretion, in 40 ± 1 ℃ of water baths, concentrating under the vacuum decompression condition then with aforementioned neutral cleansing solution, last constant volume filters, and filtrate is preserved until mensuration in refrigerator.
3. collection acidity or alkaline root secretion: on the basis of above-mentioned 1. item, successively with distilled water and deionized water rinsing several, resin cation or the resin anion (R.A.) vial handled will be housed then again, and insert connecting pipeline, following process together 2.;
4. neutral, acid, alkaline root secretion is collected simultaneously: collection process is with 2., and the three layers of resin dealt with separately of just packing in the vial, are measured respectively after concentrating, filtering last layering wash-out.
5. above-mentioned Xian takes off, concentrates and filter process is to use cleansing solution separately to wash out root secretion respectively for several times, each several part root secretion solution is being concentrated into 15mL under the vacuum decompression condition respectively in 40 ± 1 ℃ of water baths, washing is settled to 20mL then, cross the acetate fiber miillpore filter of 0.45um, filtrate is preserved in refrigerator until mensuration, and the flow velocity of regulating circulation solution is 1L/h.
6. require described a kind of root exudations continuously trapping system according to aforesaid right, it is characterized in that being provided with the plant culture ware that silica sand is housed in, its bottom opening, opening part seals with rubber stopper, have through hole on the rubber stopper, insert a communicating pipe, be communicated with and be provided with the glass wool isolation between the mouth of pipe and the silica sand, communicating pipe, to be communicated to the vial that resin is housed after through a valve suitable for reading in the lower end, after the vial sealed rubber plug that has through hole that also is provided with suitable for reading, vial end opening were isolated by glass wool, to pass to Planting utensil suitable for reading through returning communicating pipe again, be provided with inflation inlet in this section pipeline, Planting utensil and vial are light tight wall.
7. root exudations continuously trapping system according to claim 6 is characterized in that inflation inlet is positioned at the vial centre-height.
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| CN103759978A (en) * | 2014-02-10 | 2014-04-30 | 金陵科技学院 | Multi-combined device for sterile collection of plant root exudates and application thereof |
| CN104006988A (en) * | 2014-05-26 | 2014-08-27 | 上海大学 | Conical surface percolation type device and method for collecting root exudates of soil cultivation plants |
| CN104749000A (en) * | 2015-04-02 | 2015-07-01 | 上海交通大学 | Device and method for extracting helophyte root exudates |
| CN104777284A (en) * | 2015-04-13 | 2015-07-15 | 中国水稻研究所 | Measurement method for soil acid hydrolysable amino acid contents |
| CN104991050A (en) * | 2015-07-09 | 2015-10-21 | 浙江大学 | Device for measuring soil amino acid supply capacity and application |
| CN108519250A (en) * | 2018-04-18 | 2018-09-11 | 中国科学院水生生物研究所 | It is a kind of to collect submerged plant root exudates device and application method in situ |
| CN108519250B (en) * | 2018-04-18 | 2024-02-27 | 中国科学院水生生物研究所 | Device for collecting submerged plant root secretions in situ and use method |
| CN109765077A (en) * | 2019-02-28 | 2019-05-17 | 中国科学院南京土壤研究所 | A kind of root exudates sampler and sampling system |
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