CN1478889A - Production method of corn superoxide dismutase multienzyme - Google Patents
Production method of corn superoxide dismutase multienzyme Download PDFInfo
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- CN1478889A CN1478889A CNA031334822A CN03133482A CN1478889A CN 1478889 A CN1478889 A CN 1478889A CN A031334822 A CNA031334822 A CN A031334822A CN 03133482 A CN03133482 A CN 03133482A CN 1478889 A CN1478889 A CN 1478889A
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- superoxide dismutase
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Abstract
A process for preparing corn superoxide dismutase (SOD) multienzyme includes such steps as preparing raw materials, removing impurities, immersing, activating, germinating, pulping, filtering to remove bacteria, recovering alcohol, and drying. Its advantages are low cost, high enzyme activity and less consumption of water.
Description
Technical field
The present invention relates to the production method of superoxide-dismutase multienzyme, exactly is the production method of corn superoxide dismutase from corn multienzyme.
Background technology
The superoxide-dismutase multienzyme is meant superoxide-dismutase and Selenoperoxidase, catalatic mixture.This mixture is effectively to remove superoxide anion-free radical in the body to the main effect of human body, reduces cells in vivo damage or dead, thereby reduces the disease generation and delay senility.The method of existing production superoxide dismutase multienzyme mainly contains two classes, and a class is to extract from pluck, and another kind of is to extract from plants such as corn, and the method for extraction superoxide-dismutase multienzyme mainly is " salting-out process " from corn.This method is at many books and periodicals, for example all on the books in the biological chemistry study course that compile in collaboration with, Wang Jinran in Shen.The subject matter that exists when utilizing this method to produce the superoxide dismutase multienzyme is: 1, because this method is with phosphoric acid salt, sodium salt, the mixture of salts substances such as sylvite and fresh water is as extracting solution, thereby the Freshwater resources of one side mass consumption worsening shortages, on the other hand in removing work in-process during institute's salt concentration, at least need twice to analyse the salt operation again, thereby technology more complicated, at last when discharge water salt mixed solution, if earlier it is carried out purifying treatment, discharging then, then certainly will improve the manufacturing cost of product, if it is not carried out just directly discharging of purifying treatment, can pollute environment again; 2, in the drying products process, this method has adopted lyophilize or spray drying technology, and the former cost is higher, and the loss alive of latter's enzyme is bigger, and both all are not suitable for the needs of scale operation.
Summary of the invention
The objective of the invention is to provide a kind of production method of corn superoxide dismutase from corn multienzyme for people, so that produce in the described multienzyme process in this method of application, can save Freshwater resources, simplify production technique, reduce manufacturing cost, reduce enzyme loss alive, thereby overcome the problems referred to above that prior art exists.
The production method of corn superoxide dismutase from corn multienzyme provided by the invention is made up of following steps:
A, get the raw materials ready
Get an amount of raw material corn ready;
B, removal of impurities, immersion
Remove impurity in the raw material corn, this corn is inserted in the round-robin tap water soak again, soak time is 3-8 hour, takes out standby then;
C, activation, germination
It is that 0.01-0.03%, temperature are to soak in 15-30 ℃ the red enzyme cellulose solution that corn after will soaking through process B is inserted concentration, soak time is 2-10 hour, taking out corn then and it is inserted room temp is in 20-30 ℃ the malting floor, naturally deposited 15-24 hour, make it to germinate, take out standby thereafter;
D, slurrying
Adding concentration in the germination corn is the ethanol of 20-95%, and to make germination corn and alcoholic acid ratio of weight and number be 1: 2-4, utilize disintegrating apparatus that germination corn and ethanol powder mix are broken into corn steep liquor again;
E, filtration sterilization
With described corn steep liquor stir the companion evenly after, filter with first filter, remove filter residue, collect filtrate just, continue and with filter membrane first filtrate carried out essence and filter, and remove filter residue, collect smart filtrate; Smart filtrate is passed through in molecular weight is the ultrafiltration system of 15-30 ten thousand, remove filter residue again, collect final filtrate and add antioxidant therein, the add-on of this antioxidant is the 0.01-0.8% of final filtrate weight, makes sterile liquid thus.
F, recovery ethanol
Make ethanol evaporation in the described sterile liquid by vacuum-evaporator, and with its recovery, in order to reusing, regather remaining liquid, this liquid becomes corn superoxide dismutase from corn multienzyme liquid;
G, drying
With described corn superoxide dismutase from corn multienzyme liquid with insert in the loft drier through the abundant mixing of active carrier modified, after loose, under 40-90 ℃ of condition dry 1-6 hour, promptly make corn superoxide dismutase from corn multienzyme pulvis.
Above-mentioned antioxidant is the tea Dove.
Above-mentioned antioxidant is VC.
Above-mentioned active carrier is a starch.
Above-mentioned active carrier is a polysaccharide.
Above-mentioned loft drier is a vacuum drying oven.
Above-mentioned loft drier is common loft drier.
Above-mentioned filter membrane is a metal filtration film.
Above-mentioned filter membrane is a ceramic filtration membrane.
The present invention compared with prior art has following advantage after realizing: 1, the present invention adopts ethanol as extracting solution, thereby can save Freshwater resources in a large number on the one hand, make again on the other hand the operation letter of reclaiming extracting solution few to a procedure be evaporization process, and the ethanol after reclaiming also can reuse, thereby not only simplify production technique, reduce production costs, and reduce environmental pollution; 2, in the product drying process,, thereby can not only reduce drying cost significantly and can effectively improve enzyme activity because the present invention is drying plant with the loft drier.Test shows, when storing product of the present invention under 45 ℃ of conditions, its enzyme activity was not seen reduction in 60 days, and when storing product of the present invention at ambient temperature, its enzyme activity was not seen reduction in 24 months.
Embodiment
Embodiment one
A, get the raw materials ready
Get raw material corn 300kg ready;
B, removal of impurities, immersion
Remove impurity in the raw material corn, this corn is inserted in the round-robin tap water soak again, soak time is 8 hours, takes out standby then;
C, activation, germination
Corn after will soaking through process B inserts that concentration is 0.01%, temperature is to soak in 30 ℃ the red enzyme cellulose solution, soak time is 2 hours, and taking out corn then and it is inserted room temp is in 30 ℃ the malting floor, deposits naturally 15 hours, make it to germinate, take out standby thereafter;
D, slurrying
In the germination corn, add concentration and be 20% ethanol, and to make germination corn and alcoholic acid ratio of weight and number be 1: 4, utilize pulverizer that germination corn and ethanol powder mix are broken into corn steep liquor again;
E, filtration sterilization
With described corn steep liquor stir the companion evenly after, filter with first filter, remove filter residue, collect filtrate just, continue and with filter membrane first filtrate carried out essence and filter, and remove filter residue, collect smart filtrate; Smart filtrate is passed through in molecular weight is the ultrafiltration system of 15-30 ten thousand, remove filter residue again, collect final filtrate and add antioxidant therein, the add-on of this antioxidant is 0.08% of a final filtrate weight, makes sterile liquid thus.
F, recovery ethanol
Make ethanol evaporation in the described sterile liquid by vacuum-evaporator, and with its recovery, in order to reusing, regather remaining liquid, this liquid becomes corn superoxide dismutase from corn multienzyme liquid;
G, drying
With described corn superoxide dismutase from corn multienzyme liquid with insert in the loft drier through the abundant mixing of active carrier modified, after loose, under 60 ℃ of conditions dry 6 hours, promptly make corn superoxide dismutase from corn multienzyme pulvis.
Embodiment two
A, get the raw materials ready
Get raw material corn 300kg ready;
B, removal of impurities, immersion
Remove impurity in the raw material corn, corn is immersed in the round-robin tap water soak again, soak time is 3 hours, takes out standby then;
C, activation, germination
Corn after will soaking through process B inserts that concentration is 0.03%, temperature is to soak in 26 ℃ the red enzyme cellulose solution, soak time is 2 hours, and taking out corn then and it is inserted room temp is in 26 ℃ the malting floor, deposits naturally 24 hours, make it to germinate, take out standby thereafter;
D, slurrying
In the germination corn, add concentration and be 55% ethanol, and to make germination corn and alcoholic acid ratio of weight and number be 1: 2, utilize pulverizer that germination corn and ethanol powder mix are broken into corn steep liquor again, take out standby then;
E, filtration sterilization
With described corn steep liquor stir the companion evenly after, filter with first filter, remove filter residue, collect filtrate just, continue and with filter membrane first filtrate carried out essence and filter, and remove filter residue, collect smart filtrate; Smart filtrate is passed through in molecular weight is the ultrafiltration system of 15-3 ten thousand, remove filter residue again, collect final filtrate and add antioxidant therein, the add-on of this antioxidant is 0.8% of a final filtrate weight, makes sterile liquid thus.
F, recovery ethanol
Make ethanol evaporation in the described sterile liquid by vacuum-evaporator, and with its recovery, in order to reusing, regather remaining liquid, this liquid becomes corn superoxide dismutase from corn multienzyme liquid;
G, drying
Will described corn superoxide dismutase from corn multienzyme liquid with through inserting in the loft drier behind the abundant mixing of modifying of active carrier, under 80 ℃ of conditions dry 5 hours, promptly make corn superoxide dismutase from corn multienzyme pulvis.
Embodiment three
A, get the raw materials ready
Get raw material corn 300kg ready;
B, removal of impurities
Remove impurity in the raw material corn, corn is inserted in the round-robin tap water soak again, soak time is 5 hours, takes out standby then;
C, activation, germination
Corn after will soaking through process B inserts that concentration is 0.02%, temperature is to soak in 23 ℃ the red enzyme cellulose solution, soak time is 6 hours, and taking out corn then and it is inserted room temp is in 25 ℃ the malting floor, deposits naturally 20 hours, make it to germinate, take out standby thereafter;
D, slurrying
In the germination corn, add concentration and be 60% ethanol, and to make germination corn and alcoholic acid ratio of weight and number be 1: 3, utilize pulverizer that germination corn and ethanol powder mix are broken into corn steep liquor again, take out standby then;
E, filtration sterilization
With described corn steep liquor stir the companion evenly after, filter with first filter, remove filter residue, collect filtrate just, continue and with filter membrane first filtrate carried out essence and filter, and remove filter residue, collect smart filtrate; Smart filtrate is passed through in molecular weight is the ultrafiltration system of 15-30 ten thousand, remove filter residue again, collect final filtrate and add antioxidant therein, the add-on of this antioxidant is 0.4% of a final filtrate weight, makes sterile liquid thus.
F, recovery ethanol
Make ethanol evaporation in the described sterile liquid by vacuum-evaporator, and with its recovery, in order to reusing, regather remaining liquid, this liquid becomes corn superoxide dismutase from corn multienzyme liquid;
G, drying
With described corn superoxide dismutase from corn multienzyme liquid with insert in the loft drier through the abundant mixing of active carrier modified, after loose, under 90 ℃ of conditions dry 4 hours, promptly make corn superoxide dismutase from corn multienzyme pulvis.
Should illustrate that in implementing process of the present invention, described antioxidant promptly can be that the tea Dove also can be VC; Described active carrier promptly can be a starch, also can be polysaccharide; Described loft drier promptly can be a vacuum drying oven, also can be common loft drier; Described filter membrane promptly can be a metal filtration film, also can be ceramic filtration membrane.
Claims (9)
1, the production method of corn superoxide dismutase from corn multienzyme is characterized in that it is made up of following process:
A, get the raw materials ready
Get an amount of raw material corn ready;
B, removal of impurities, immersion
Remove impurity in the raw material corn, this corn is inserted in the round-robin tap water soak again, soak time is 3-8 hour, takes out standby then;
C, activation, germination
It is that 0.01-0.03%, temperature are to soak in 15-30 ℃ the red enzyme cellulose solution that corn after will soaking through process B is inserted concentration, soak time is 2-10 hour, taking out corn then and it is inserted room temp is in 20-30 ℃ the malting floor, naturally deposited 15-24 hour, make it to germinate, take out standby thereafter;
D, slurrying
Adding concentration in the germination corn is the ethanol of 20-95%, and to make germination corn and alcoholic acid ratio of weight and number be 1: 2-4, utilize disintegrating apparatus that germination corn and ethanol powder mix are broken into corn steep liquor again;
E, filtration sterilization
With described corn steep liquor stir the companion evenly after, filter with first filter, remove filter residue, collect filtrate just, continue and with filter membrane first filtrate carried out essence and filter, and remove filter residue, collect smart filtrate; Make then
Smart filtrate is passed through in molecular weight is the ultrafiltration system of 15-30 ten thousand, removes filter residue again, collects final filtrate and adds antioxidant therein, and the add-on of this antioxidant is the 0.01-0.8% of final filtrate weight, makes sterile liquid thus;
F, recovery ethanol
Make ethanol evaporation in the described sterile liquid by vacuum-evaporator, and with its recovery, in order to reusing, regather remaining liquid, this liquid becomes corn superoxide dismutase from corn multienzyme liquid;
G, drying
With described corn superoxide dismutase from corn multienzyme liquid with insert in the loft drier through the abundant mixing of active carrier modified, after loose, under 40-90 ℃ of condition dry 1-6 hour, promptly make corn superoxide dismutase from corn multienzyme pulvis.
2, the production method of corn superoxide dismutase from corn multienzyme according to claim 1 is characterized in that described antioxidant is the tea Dove.
3, the production method of corn superoxide dismutase from corn multienzyme according to claim 1 is characterized in that described antioxidant is VC.
4, the production method of corn superoxide dismutase from corn multienzyme according to claim 1 is characterized in that described active carrier is a starch.
5, the production method of corn superoxide dismutase from corn multienzyme according to claim 1 is characterized in that described active carrier is a polysaccharide.
6, the production method of corn superoxide dismutase from corn multienzyme according to claim 1 is characterized in that described loft drier is a vacuum drying oven.
7, the production method of corn superoxide dismutase from corn multienzyme according to claim 1 is characterized in that described loft drier is common loft drier.
8, the production method of corn superoxide dismutase from corn multienzyme according to claim 1 is characterized in that described filter membrane is a metal filtration film.
9, the production method of corn superoxide dismutase from corn multienzyme according to claim 1 is characterized in that described filter membrane is a ceramic filtration membrane.
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| CN 03133482 CN1215169C (en) | 2003-06-20 | 2003-06-20 | Production method of corn superoxide dismutase multienzyme |
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| CN 03133482 CN1215169C (en) | 2003-06-20 | 2003-06-20 | Production method of corn superoxide dismutase multienzyme |
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| CN1478889A true CN1478889A (en) | 2004-03-03 |
| CN1215169C CN1215169C (en) | 2005-08-17 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1329509C (en) * | 2004-06-17 | 2007-08-01 | 天津科技大学 | Maize coupled alcohol fermentation and superoxide dismutase extraction processing method |
| CN101892204A (en) * | 2010-06-18 | 2010-11-24 | 李作红 | Method for preparing corn superoxide dismutase from corn |
| CN103421750A (en) * | 2012-05-23 | 2013-12-04 | 王兆非 | Industrial production method for preparing superoxide dismutase compound enzymes with corn as raw material and sprouting room |
| CN113373122A (en) * | 2021-07-29 | 2021-09-10 | 辽宁未来生物科技有限公司 | Preparation method of novel corn superoxide dismutase multienzyme |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104774812A (en) * | 2014-10-09 | 2015-07-15 | 湖南一九生物科技有限公司 | Processing method for extraction of superoxide dismutase from corn germ |
-
2003
- 2003-06-20 CN CN 03133482 patent/CN1215169C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1329509C (en) * | 2004-06-17 | 2007-08-01 | 天津科技大学 | Maize coupled alcohol fermentation and superoxide dismutase extraction processing method |
| CN101892204A (en) * | 2010-06-18 | 2010-11-24 | 李作红 | Method for preparing corn superoxide dismutase from corn |
| CN103421750A (en) * | 2012-05-23 | 2013-12-04 | 王兆非 | Industrial production method for preparing superoxide dismutase compound enzymes with corn as raw material and sprouting room |
| CN103421750B (en) * | 2012-05-23 | 2018-12-28 | 王兆非 | Industrialized preparing process and its rudiment room of superoxide dismutase complex enzyme are prepared using corn as raw material |
| CN113373122A (en) * | 2021-07-29 | 2021-09-10 | 辽宁未来生物科技有限公司 | Preparation method of novel corn superoxide dismutase multienzyme |
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| CN1215169C (en) | 2005-08-17 |
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