CN1472526A - Tunnel capillary electrophoretic chemiluminescence testing microfluid control chip - Google Patents
Tunnel capillary electrophoretic chemiluminescence testing microfluid control chip Download PDFInfo
- Publication number
- CN1472526A CN1472526A CNA021258643A CN02125864A CN1472526A CN 1472526 A CN1472526 A CN 1472526A CN A021258643 A CNA021258643 A CN A021258643A CN 02125864 A CN02125864 A CN 02125864A CN 1472526 A CN1472526 A CN 1472526A
- Authority
- CN
- China
- Prior art keywords
- sample
- tunnel
- pool
- liquid pool
- cover plate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The chip includes base-tab and cover sheet as buffer liquid pool, at least two sample pools, at least two reagent pools, waste liquid pool and reaction detecting pool are set on the base-tab, of which two sample pools are connected to sample inlet channel, buffer liquid pool is connected with separation channel via sample inlet channel after it has been crossed with sample inlet channel of sample pool The separation channel, reagent pool, waste liquid pool are connected with reaction detecting pool. In addition, liquid pool holes corresponding to pools of sample, buffer liquid, reagent, waste liquid and reaction detecting are set on the cover sheet. The chip is finalized by integrating the base-tab and cover sheet together.
Description
Technical field
The present invention relates to a kind of glass or transparent high polymer micro-flow control chips of suitable chemiluminescence detection, particularly is the separation of the material that can carry out chemiluminescence reaction such as a kind of suitable micro-fluorescence organic compound, metallic ion and the micro-fluidic chip that detects usefulness.
Background technology
Science and technology development require analysis science with lower cost, easier method, more direct approach, more efficiently mode provide be concerned about the information of object.The micro-full analytical system that the early 1990s occurs is the revolution of analytical chemistry, and it is integrated in unit operationss such as the sample pretreatment of traditional analysis chemical laboratory, reaction, separation, detection on the microchip, disposablely finishes whole operations.Used process technology derives from the semiconductor microactuator process technology, promptly on glass or piezoid, adopt optical etching technology to produce the microstructure that some microchannels, micro reaction pool etc. satisfy different needs, finish the chemical analysis task, be called " laboratory on the chip ".Because a large amount of reagent and sample have been saved in the application of micro-full analytical system, have accelerated analysis speed, have improved work efficiency, have simplified routine analyzer, pollution and the error of having avoided intermediate link to cause.The report of micro-fluidic chip that is applied to compartment analysis at present is a lot, and detection method is ultraviolet detection, laser-Induced Fluorescence Detection, Electrochemical Detection, Mass Spectrometer Method etc.Laser-Induced Fluorescence Detection and Mass Spectrometer Method have very high sensitivity, but because the instrument volume is bigger, are difficult to integratedly, are unfavorable for system's microminiaturization.The sensitivity of ultraviolet detection is too low.Though Electrochemical Detection has very high sensitivity, its application is restricted owing to separate the influence of highfield.Advantages such as that chemiluminescence detecting method has is highly sensitive, it is integrated to be easy to, low cost of manufacture are the detection meanss of desirable micro-full analytical system.
At present disclosed have following several with chemiluminescence as the micro-fluidic chip of detection means:
(1) three dimension microchannel biochip chemiluminescence is carried out hydridization and is measured: make chip with standard photoetching legal system, to chip microchannel surface silicon alkanisation, will survey immobilization of reagents then in the microchannel inside surface, promptly can be used for hydridization and measure.Measurement standard deviation to 64 points is 8.1%, and detectability reaches 250amol, and linear range is 3 order of magnitude (B.J.Cheek, A.B.Steel, M.P.Torres, Y.-Y Yu, H.Yang, Chemiluminescence detection for hybridization assays on theflow-thru chip, a three-dimensional microchannel biochip, Anal.Chem., 2001,73,5777-5783.).
(2) chemiluminescence testing microfluid control chip is used for the mensuration of transition metal ion: human peristaltic pump continuous sample introductions such as X.-J.Huang, on chip platform, carry out capillary electrophoresis separation, enter the luminescence reagent pond after the sample separation and carry out chemiluminescence reaction, this is not proper micro-fluidic chip, but capillary electrophoresis-chemiluminescence detects.People such as Tsukagoshi use the most classical cruciform micro-fluidic electrophoresis chip, short passage is used for sample introduction, and another microchannel is exactly a split tunnel, and the liquid pool of split tunnel end injects luminescence reagent, when metallic ion when split tunnel enters this liquid pool, chemiluminescence reaction takes place.The mensuration of Co (II) shows to have good reappearance, measures 6 times, and the standard deviation of transit time and peak height is no more than 5%, and detectability 0.4fmol is 1.0 * 10
-5-1.0 * 10
-3Has good correlativity in the M scope.But, because luminescence reagent is stored in the liquid pool, can't carry out immediate updating in the mensuration process, and waste reaction solution amassing in liquid pool, can not discharge immediately, the mensuration of follow-up metallic ion is produced disturb.(X.-J.Huang，Q.-S.Pu，Z.-L.Fang，Capillary?electro-phoresis?system?with?flow?injection?sampleintroduction?and?chemi-luminescence?detection?on?a?chip?platform，Analyst.2001，126，281-284.K.Tsukagoshi，M.Hashimoto，R.Nakajima，A.Arai，Application?of?microchip?capillaryelectrophoresis?with?chemiluminescence?detection?to?analysis?fortransition-metal?ions.Anal.Sci.，2000，16，1111-1112)。
Summary of the invention
The objective of the invention is for a kind of micro-fluidic chip that is applicable to electrophoretic separation chemiluminescence detection such as organic fluorescence compound, transition metal ion is provided; This chip has long split tunnel and reaction detection pond cleverly, thus have that higher sensitivity and separation efficiency, interference are little, characteristics such as reagent immediate updating, polycomponent mensuration and equipment are simple; This micro-fluidic chip low cost of manufacture, be easy to produce in batches.
Purpose of the present invention can realize by following measure:
A kind of tunnel capillary electrophoretic chemiluminescence testing microfluid control chip comprises substrate and cover plate; On substrate, establish buffer pool D, at least two sample cell A, B, at least two reagent pond E, F, waste liquid pool G and reaction detection pond H; Wherein two sample cell A, B are communicated with by sample intake passage, and buffer pool D links to each other with split tunnel after the sample intake passage of sample intake passage and sample cell A, B intersects; Described split tunnel, reagent pond E, F and waste liquid pool G all link to each other with reaction detection pond H; On cover plate, establish the liquid pool hole in addition, then substrate and cover plate are fixed into chip with the corresponding position of on-chip sample cell A, B, buffer pool D, reagent pond E, F, waste liquid pool G and reaction detection pond H.
When in described sample cell A, the energising of B two ends, sample is promptly switched between described buffer pool D, reagent pond E, F and waste liquid pool G after being manoeuvred into the crossing C of sample intake passage and split tunnel along sample intake passage; Simultaneously sample cell A, B two ends are floated or add same equipotential.Sample in split tunnel under electric field action, move and separate along split tunnel, when arriving reaction detection pond H, with the concurrent hair tonic light reaction of reagent mix of under electric field action, coming from reagent pond E, F conveying, light signal receives by placing the receptacle under the H of reaction detection pond, and the waste liquid that the reaction back produces enters waste liquid pool G under electric field action.
A kind of in the groove that above-mentioned split tunnel is helicla flute, straight-line groove, curved groove, broken line groove, straight line links to each other with curve and/or broken line, the groove that curve links to each other with curve, groove that curve links to each other with broken line and curve and straight line and the groove that broken line links to each other.
The above-mentioned substrate and the material of cover plate are selected from glass and poly-methyl-prop diluted acid formicester (PMMA), dimethyl silicone polymer transparency superpolymer (PDMS), polystyrene, Polyvinylchloride, phenolics, epoxy resin, polyurethane, poly-several lactams or any multipolymer between them.
Liquid pool hole on the above-mentioned cover plate is a through hole.
The present invention has following advantage than prior art:
The present invention combines chemiluminescence detection and micro-fluidic electrophoresis chip, compare with known chemiluminescence testing microfluid control chip, long split tunnel and reaction detection pond are cleverly arranged, thus have that higher sensitivity and separation efficiency, interference are little, characteristics such as reagent immediate updating, polycomponent mensuration and equipment are simple.This micro-fluidic chip low cost of manufacture, be easy to produce in batches.Mensuration for materials such as fluorescence organic compound, transition metal ion in seawater and the environmental pollution water has broad application prospects.
Description of drawings
Fig. 1 is a structural representation of the present invention
1-substrate 2-buffer pool D 3-crossing C 4-sample cell A, B
5-sample intake passage 6-split tunnel 7-reagent pond E, F
H 9-waste liquid pool G 10-cover plate 11-liquid pool hole, 8-reaction detection pond
Fig. 2 is the structural representation of substrate of the present invention
Fig. 3 is the structural representation of cover plate of the present invention
Fig. 4 is a decomposing schematic representation of the present invention
Embodiment
Embodiment 1:
(1) making of substrate: the H that glass plate is at first boiling
2SO
4: H
2O
2Volume ratio is cleaned in 4: 1, respectively carries out ultrasonic cleaning 10 minutes with acetone, alcohol, high purity water successively again.Carry out vacuum coating then, plate the chromium film of 150nm, carrying out the photoresists coating again is the PMER positive photoresist, 600 rev/mins, 2 minutes, about 1 micron of thickness, 95 ℃ of following preliminary drying half an hour, cooling, the mask film that will have design drawing places on the photoresist, ultraviolet wavelength is 365nm exposure 90 seconds, develops 2 minutes, and then dries by the fire half an hour down at 100 ℃.At room temperature use chromium film etching liquid, the proportioning that etching is explained is a cerous sulfate: perchloric acid: water=50 grams: 15 milliliters: 300 milliliters, and corrosion chromium film, water is rinsed well then.With 0.4M HF/0.4M NH
4F corrodes Pyrex, and corrosion speed is 6 microns/hour.After the etching ten hours, washing, acetone is washed, and removes the film that dechromises with chromium film etching liquid.Be substrate.Waste liquid pool is gone to the bottom 100 microns to 220 microns of detection cell passage and sample intake passage width upper bases, 60 microns of the degree of depth, and 170 microns of the last bottom width of split tunnel and reagent passage, 50 microns of following bottom width, 60 microns of the degree of depth, split tunnel length are 21 centimetres.
(2) making of cover plate, with the glass sheet of the same size of the same material of substrate on, selected and the corresponding position of substrate liquid pool, with miniature bench drill punching, the diameter in hole depends on the diameter of drill bit, and when drill bit was 1 millimeter a diamond bit, the diameter in hole was 1 millimeter of liquid pool diameter.
(3) bonding: with substrate and cover plate at H
2SO
4: H
2O
2Volume ratio 4: 1, H
2O: H
2O
2: NH
4OH volume ratio 50: 10: 1, H
2O: H
2O
2: ultrasonic cleaning is 10 minutes in the HCl volume ratio 5: 1: 1, dry up with nitrogen, water glass (is contained silicon dioxide 35-38%, 17-19% is received in oxidation, small amounts calcium) evenly be coated on cover plate surface (about 2000 rev/mins of rotating speed with photoresist spinner, applied 20 seconds), then two are closed up, under nitrogen environment, be heated to 80 ℃ and keep about 1 hour.Obtain the micro-fluidic glass-chip that microstructure parameters equals above-mentioned cover plate and substrate.
Embodiment 2:
(1) the glass mold plungers plate is made: the H that glass plate is at first boiling
2SO
4: H
2O
2Volume ratio is cleaned in 4: 1, respectively carries out ultrasonic cleaning 10 minutes with acetone, alcohol, high purity water successively again.Carry out vacuum coating then, plate the chromium film of 150nm, carry out photoresists coating (PMER positive photoresist, 600 rev/mins again, 2 minutes, about 1 micron of thickness), 95 ℃ of following preliminary dryings 30 minutes, cooling, the mask film (with the mask used inverse of glass-chip) that will have design drawing places on the photoresist, ultraviolet wavelength is 365nm exposure 90 seconds, develops 2 minutes, and then dries by the fire half an hour down at 100 ℃.(cerous sulfate: perchloric acid: corrosion chromium film water=50 grams: 15 milliliters: 300 milliliters), water is rinsed well then at room temperature to use chromium film etching liquid.With 0.2M HF/0.2M NH
4F corrodes Pyrex, and corrosion speed is 4 microns/hour.After the etching 15 hours, washing, acetone is washed, and removes the film that dechromises with chromium film etching liquid.Be the substrate formpiston that has convex microchannel figure.Waste liquid pool is gone to the bottom 200 microns to 80 microns of detection cell passage and sample intake passage width upper bases, 60 microns of height, and 30 microns of the last bottom width of split tunnel and reagent passage, 150 microns of following bottom width, highly 60 microns, split tunnel length is 21 centimetres.
(2) the synthetic PMMA chip substrate of bulk polymerization: the metering system methyl esters (MMA) after will purifying is with after initiating agent (being generally benzoyl peroxide or azo-bis-isobutyl cyanide) mixed in 1000: 5 by volume; 85-90 ℃ of prepolymerization about 50 minutes; be cooled to room temperature rapidly; vacuum outgas 30 minutes; be cast to then on the formpiston of step (1); 45 ℃ of polymerizations 12 hours; be warming up to 95 ℃ of polymerizations 2 hours then; slowly cool to the back demoulding about 40 ℃; get PMMA micro-fluidic chip substrate, channel size is corresponding to force plate.
(3) cover plate is made: with diameter is 3 millimeters, highly is that 2.5 millimeters stainless steel column places bright and clean glass ad-hoc location, makes mould, adopts the method identical with this subject polymerization PMMA substrate to synthesize cover plate, after the demoulding, take out little steel column cover plate.
(4) bonding: substrate alignd with cover plate close up, 110 ℃ of bondings 2 hours, the PMMA micro-fluidic chip, waste liquid pool is gone to the bottom 80 microns to 200 microns of detection cell passage and sample intake passage width upper bases, 60 microns of height, 150 microns of the last bottom width of split tunnel and reagent passage, 30 microns of following bottom width, 60 microns of height, split tunnel length is 21 centimetres.
Embodiment 3:
(1) the glass mold plungers plate is made: the H that glass plate is at first boiling
2SO
4: H
2O
2Clean in (4: 1), respectively carried out ultrasonic cleaning 10 minutes with acetone, alcohol, high purity water successively again.Carry out vacuum coating then, plate the chromium film of 150nm, carry out photoresists coating (PMER positive photoresist, 600 rev/mins again, 2 minutes, about 1 micron of thickness), 95 ℃ of following preliminary drying half an hour, cooling, the mask film (with the mask used inverse of glass-chip) that will have design drawing places on the photoresist, uv-exposure 90 seconds (wavelength 365nm) developed 2 minutes, and then dried by the fire half an hour down at 100 ℃.(cerous sulfate: perchloric acid: corrosion chromium film water=50 grams: 15 milliliters: 300 milliliters), water is rinsed well then at room temperature to use chromium film etching liquid.Use 0.2MHF/0.2MNH
4F corrodes Pyrex, and corrosion speed is 4 microns/hour.After the etching 15 hours, washing, acetone is washed, and removes the film that dechromises with chromium film etching liquid.Be the substrate formpiston that has convex microchannel figure.Waste liquid pool is gone to the bottom 200 microns to 80 microns of detection cell passage and sample intake passage width upper bases, 60 microns of height, and 30 microns of the last bottom width of split tunnel and reagent passage, 150 microns of following bottom width, highly 60 microns, split tunnel length is 21 centimetres.
(2) the synthetic PDMS chip substrate of bulk polymerization: (Sylgard 184, Dow Corning, Midland with PDMS monomer and initiating agent, MI) mixed by weight 10: 1, be cast on the force plate of step (1), 65 ℃ of polymerizations about 1 hour, cooling, lift-off stencil promptly gets the PDMS substrate;
(3) cover plate is made: with diameter is 3 millimeters, highly is that 2.5 millimeters stainless steel column places bright and clean glass ad-hoc location, makes mould, adopts the method identical with this subject polymerization PDMS substrate to synthesize cover plate, after the demoulding, take out little steel column cover plate.
(4) bonding: substrate and cover plate ethanol wash clean, dry up with argon gas, be put in the air plasma device oxidation after 1 minute, immediately two are closed up, cover plate and substrate are firm combining, make the PDMS micro-fluidic chip, or utilize and add certain pressure under the viscosity room temperature of PDMS material and carry out reversible keying, waste liquid pool is gone to the bottom 80 microns to 200 microns of detection cell passage and sample intake passage width upper bases, 60 microns of height, 150 microns of the last bottom width of split tunnel and reagent passage, 30 microns of following bottom width, 60 microns of height, split tunnel length is 21 centimetres.
Claims (5)
1, a kind of tunnel capillary electrophoretic chemiluminescence testing microfluid control chip comprises substrate (1) and cover plate (10); It is characterized in that on substrate (1), establishing buffer pool D (2), at least two sample cell A, B (4), at least two reagent pond E, F (7), waste liquid pool G (9) and reaction detection pond H (8); Wherein two sample cell A, B (4) are communicated with by sample intake passage (5), buffer pool D (2) through sample intake passage (5) with link to each other with split tunnel (6) after the sample intake passage (5) of sample cell A, B (4) is crossing; Described split tunnel (6), reagent pond E, F (7) and waste liquid pool G (9) all link to each other with reaction detection pond H (8); In addition cover plate (10) go up and substrate (1) on the corresponding position of sample cell A, B (4), buffer pool D (2), reagent pond E, F (7), waste liquid pool G (9) and reaction detection pond H (8) establish liquid pool hole (11), then substrate (1) and cover plate (10) are fixed into chip.
2, tunnel capillary electrophoretic chemiluminescence testing microfluid control chip as claimed in claim 1 is characterized in that a kind of in helicla flute, straight-line groove, curved groove, broken line groove, groove that straight line links to each other with curve and/or broken line, groove that curve links to each other with curve, groove that curve links to each other with broken line and curve and straight line and the groove that broken line links to each other of described split tunnel (6).
3, tunnel capillary electrophoretic chemiluminescence testing microfluid control chip as claimed in claim 1 is characterized in that the material of described substrate (1) and cover plate (10) is selected from glass and poly-methyl-prop diluted acid formicester, dimethyl silicone polymer transparency superpolymer, polystyrene, Polyvinylchloride, phenolics, epoxy resin, polyurethane, poly-several lactams or any multipolymer between them.
4, tunnel capillary electrophoretic chemiluminescence testing microfluid control chip as claimed in claim 1 is characterized in that the liquid pool hole A on the described cover plate (10), B, and D, E, F, G (11) is a through hole.
5, tunnel capillary electrophoretic chemiluminescence testing microfluid control chip as claimed in claim 1, it is characterized in that when the time in described sample cell A, the energising of B (4) two ends, after sample is manoeuvred into the crossing C (3) of sample intake passage (5) and split tunnel (6) along sample intake passage (5), i.e. energising between described buffer pool D (2), reagent pond E, F (7) and waste liquid pool G (9); Simultaneously sample cell A, B (4) two ends are floated or add same equipotential.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02125864 CN1206527C (en) | 2002-07-31 | 2002-07-31 | Tunnel capillary electrophoretic chemiluminescence testing microfluid control chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02125864 CN1206527C (en) | 2002-07-31 | 2002-07-31 | Tunnel capillary electrophoretic chemiluminescence testing microfluid control chip |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1472526A true CN1472526A (en) | 2004-02-04 |
| CN1206527C CN1206527C (en) | 2005-06-15 |
Family
ID=34143115
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02125864 Expired - Fee Related CN1206527C (en) | 2002-07-31 | 2002-07-31 | Tunnel capillary electrophoretic chemiluminescence testing microfluid control chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1206527C (en) |
Cited By (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100380126C (en) * | 2004-04-16 | 2008-04-09 | 南京大学 | Microcurrent controlled chip detection combiner having general and integral and high efficiency radiation |
| CN100582743C (en) * | 2006-12-21 | 2010-01-20 | 中国科学院半导体研究所 | Optical biochip for disease diagnosis and preparation method thereof |
| WO2010135852A1 (en) * | 2009-05-27 | 2010-12-02 | 西门子公司 | Capillary electrophoresis chip, apparatus and method suitable for online application |
| CN1928534B (en) * | 2006-09-29 | 2011-02-09 | 西安交通大学 | Method for preparing micro-flowing injection type chemical luminous chip |
| CN101126765B (en) * | 2007-10-09 | 2011-02-16 | 中国科学院理化技术研究所 | Microfluid sample boat |
| CN101692047B (en) * | 2009-10-27 | 2011-10-05 | 浙江大学 | Microfluidic chip for capillary electrophoresis separation and chemiluminescence detection |
| CN102513170A (en) * | 2004-09-09 | 2012-06-27 | 居里研究所 | A device for manipulation of packets in micro-containers, in particular in microchannels |
| CN102866147A (en) * | 2012-09-05 | 2013-01-09 | 重庆医科大学 | Aptamer-based micro-fluidic chemiluminescence chip and preparation method for same |
| CN103033608A (en) * | 2011-10-10 | 2013-04-10 | 中山大学 | Integration micro-fluidic chip for immune analysis research and applications thereof |
| CN103301891A (en) * | 2013-05-27 | 2013-09-18 | 苏州扬清芯片科技有限公司 | Self-heating microfluidic chip |
| CN103344631A (en) * | 2013-07-15 | 2013-10-09 | 浙江省海洋水产研究所 | Micro-fluidic chip for non-aqueous electrophoretic separation and peroxyoxalate ester chemiluminescence detection |
| CN103464229A (en) * | 2013-09-10 | 2013-12-25 | 东南大学 | Multilevel sorting microfluidic device for rare cells |
| CN104251875A (en) * | 2009-05-29 | 2014-12-31 | 生命技术公司 | Method for controlling fluid flow in fluidics circuit, and fluid control device |
| CN104772168A (en) * | 2015-03-19 | 2015-07-15 | 华南理工大学 | Micro-fluidic chip containing microfluid in self-discipline movement, manufacturing method, and liquid injecting device |
| CN104792701A (en) * | 2015-03-17 | 2015-07-22 | 中国科学院物理研究所 | Micro-sample cell |
| CN105538716A (en) * | 2016-01-08 | 2016-05-04 | 中国石油大学(北京) | Sample pool suitable for detecting high-water-content oil by terahertz spectrum and manufacturing method of sample pool |
| CN105572337A (en) * | 2016-01-14 | 2016-05-11 | 中国科学院理化技术研究所 | Self-driven microfluid detection card capable of detecting multiple targets |
| CN106238109A (en) * | 2016-07-13 | 2016-12-21 | 厦门大学 | The micro-fluidic chip of a kind of methamphetamine hydrochloride in Raman detection hair and using method thereof |
| CN108535239A (en) * | 2018-03-28 | 2018-09-14 | 上海艾瑞德生物科技有限公司 | Micro-fluidic chip based on microlayer model and detecting system |
| CN109142299A (en) * | 2018-09-08 | 2019-01-04 | 重庆科技学院 | A kind of petroleum wastewater heavy metal ion detection chip application method |
| WO2019024182A1 (en) * | 2017-07-31 | 2019-02-07 | 深圳大学 | Microfluidic chip and preparation method and test method therefor |
| CN109655447A (en) * | 2019-01-28 | 2019-04-19 | 广东海天创新技术有限公司 | Detection system and method for microorganism count |
| US11951474B2 (en) | 2008-10-22 | 2024-04-09 | Life Technologies Corporation | Fluidics systems for sequential delivery of reagents |
-
2002
- 2002-07-31 CN CN 02125864 patent/CN1206527C/en not_active Expired - Fee Related
Cited By (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100380126C (en) * | 2004-04-16 | 2008-04-09 | 南京大学 | Microcurrent controlled chip detection combiner having general and integral and high efficiency radiation |
| CN102513170B (en) * | 2004-09-09 | 2015-03-25 | 居里研究所 | A device for manipulation of packets in micro-containers, in particular in microchannels |
| CN102513170A (en) * | 2004-09-09 | 2012-06-27 | 居里研究所 | A device for manipulation of packets in micro-containers, in particular in microchannels |
| CN1928534B (en) * | 2006-09-29 | 2011-02-09 | 西安交通大学 | Method for preparing micro-flowing injection type chemical luminous chip |
| CN100582743C (en) * | 2006-12-21 | 2010-01-20 | 中国科学院半导体研究所 | Optical biochip for disease diagnosis and preparation method thereof |
| CN101126765B (en) * | 2007-10-09 | 2011-02-16 | 中国科学院理化技术研究所 | Microfluid sample boat |
| US9550183B2 (en) | 2008-10-22 | 2017-01-24 | Life Technologies Corporation | Fluidics system for sequential delivery of reagents |
| US10478816B2 (en) | 2008-10-22 | 2019-11-19 | Life Technologies Corporation | Fluidics system for sequential delivery of reagents |
| US11040344B2 (en) | 2008-10-22 | 2021-06-22 | Life Technologies Corporation | Fluidics system for sequential delivery of reagents |
| US11951474B2 (en) | 2008-10-22 | 2024-04-09 | Life Technologies Corporation | Fluidics systems for sequential delivery of reagents |
| WO2010135852A1 (en) * | 2009-05-27 | 2010-12-02 | 西门子公司 | Capillary electrophoresis chip, apparatus and method suitable for online application |
| CN104251875B (en) * | 2009-05-29 | 2017-07-18 | 生命技术公司 | Method and flow control apparatus for the fluid stream in controlling stream control loop |
| CN104251875A (en) * | 2009-05-29 | 2014-12-31 | 生命技术公司 | Method for controlling fluid flow in fluidics circuit, and fluid control device |
| CN101692047B (en) * | 2009-10-27 | 2011-10-05 | 浙江大学 | Microfluidic chip for capillary electrophoresis separation and chemiluminescence detection |
| CN103033608A (en) * | 2011-10-10 | 2013-04-10 | 中山大学 | Integration micro-fluidic chip for immune analysis research and applications thereof |
| CN102866147B (en) * | 2012-09-05 | 2015-08-26 | 重庆医科大学 | A kind of based on fit micro-fluidic chemical luminous chip and preparation method thereof |
| CN102866147A (en) * | 2012-09-05 | 2013-01-09 | 重庆医科大学 | Aptamer-based micro-fluidic chemiluminescence chip and preparation method for same |
| CN103301891A (en) * | 2013-05-27 | 2013-09-18 | 苏州扬清芯片科技有限公司 | Self-heating microfluidic chip |
| CN103344631A (en) * | 2013-07-15 | 2013-10-09 | 浙江省海洋水产研究所 | Micro-fluidic chip for non-aqueous electrophoretic separation and peroxyoxalate ester chemiluminescence detection |
| CN103464229B (en) * | 2013-09-10 | 2015-07-15 | 东南大学 | Multilevel sorting microfluidic device for rare cells |
| CN103464229A (en) * | 2013-09-10 | 2013-12-25 | 东南大学 | Multilevel sorting microfluidic device for rare cells |
| CN104792701A (en) * | 2015-03-17 | 2015-07-22 | 中国科学院物理研究所 | Micro-sample cell |
| CN104792701B (en) * | 2015-03-17 | 2017-11-21 | 中国科学院物理研究所 | Micro-example pond |
| CN104772168A (en) * | 2015-03-19 | 2015-07-15 | 华南理工大学 | Micro-fluidic chip containing microfluid in self-discipline movement, manufacturing method, and liquid injecting device |
| CN105538716A (en) * | 2016-01-08 | 2016-05-04 | 中国石油大学(北京) | Sample pool suitable for detecting high-water-content oil by terahertz spectrum and manufacturing method of sample pool |
| CN105572337A (en) * | 2016-01-14 | 2016-05-11 | 中国科学院理化技术研究所 | Self-driven microfluid detection card capable of detecting multiple targets |
| CN106238109A (en) * | 2016-07-13 | 2016-12-21 | 厦门大学 | The micro-fluidic chip of a kind of methamphetamine hydrochloride in Raman detection hair and using method thereof |
| CN106238109B (en) * | 2016-07-13 | 2018-03-27 | 厦门大学 | A kind of micro-fluidic chip and its application method for being used for methamphetamine in Raman detection hair |
| WO2019024182A1 (en) * | 2017-07-31 | 2019-02-07 | 深圳大学 | Microfluidic chip and preparation method and test method therefor |
| CN108535239A (en) * | 2018-03-28 | 2018-09-14 | 上海艾瑞德生物科技有限公司 | Micro-fluidic chip based on microlayer model and detecting system |
| CN109142299A (en) * | 2018-09-08 | 2019-01-04 | 重庆科技学院 | A kind of petroleum wastewater heavy metal ion detection chip application method |
| CN109142299B (en) * | 2018-09-08 | 2023-07-18 | 重庆科技学院 | Application method of petroleum wastewater heavy metal ion detection chip |
| CN109655447A (en) * | 2019-01-28 | 2019-04-19 | 广东海天创新技术有限公司 | Detection system and method for microorganism count |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1206527C (en) | 2005-06-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1206527C (en) | Tunnel capillary electrophoretic chemiluminescence testing microfluid control chip | |
| US5716852A (en) | Microfabricated diffusion-based chemical sensor | |
| CN101221168B (en) | Microfluidic chip based on microsphere biological detection | |
| US5948684A (en) | Simultaneous analyte determination and reference balancing in reference T-sensor devices | |
| Sato et al. | Integration of chemical and biochemical analysis systems into a glass microchip | |
| Ohashi et al. | A micro-ELISA system for the rapid and sensitive measurement of total and specific immunoglobulin E and clinical application to allergy diagnosis | |
| US20060073599A1 (en) | Microfabricated diffusion-based chemical sensor | |
| JP2003130765A (en) | Method and device for fluid treatment in substrate | |
| WO1997039338A9 (en) | Microfabricated diffusion-based chemical sensor | |
| Mawatari et al. | Microflow systems for chemical synthesis and analysis: Approaches to full integration of chemical process | |
| JP4141494B2 (en) | Microanalytical measuring apparatus and microanalytical measuring method using the same | |
| CN103191792B (en) | Microfluidic chip for microspheric multi-element biological detection | |
| CN1711209A (en) | Micro fluid device and process for producing the same | |
| CN1265199C (en) | Micro fluidic biological chip based on micro balls | |
| Nakao et al. | Femtoliter volumetric pipette and flask utilizing nanofluidics | |
| Fujii et al. | Fluorometric determination of sulfite and nitrite in aqueous samples using a novel detection unit of a microfluidic device | |
| Song et al. | Polymers for microfluidic chips | |
| Johnson et al. | Microfluidic ion-sensing devices | |
| Mawatari et al. | Extended-nano fluidic systems for analytical and chemical technologies | |
| CN202951487U (en) | Micro-fluidic chip integrating functions of micro-cavity static polymerase chain reactions (PCRs) and capillary electrophoresis (CE) | |
| KR101048858B1 (en) | Open groove channel chip | |
| KR101083976B1 (en) | Apparatus for separating sample by using microchip and method thereof | |
| Sano | Creation of Nano-sized Valves for Nanofluidic Device | |
| Beugelsdijk | Where Science Meets Silicon: Microfabrication Techniques and Their Scientific Applications | |
| Takabayashi et al. | Direct observation of dispersion and mixing processes in microfluidic systems |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |