CN1460713A - Camellia chrysantha culture medium for tissue culture and quick breeding - Google Patents
Camellia chrysantha culture medium for tissue culture and quick breeding Download PDFInfo
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- CN1460713A CN1460713A CN 03124233 CN03124233A CN1460713A CN 1460713 A CN1460713 A CN 1460713A CN 03124233 CN03124233 CN 03124233 CN 03124233 A CN03124233 A CN 03124233A CN 1460713 A CN1460713 A CN 1460713A
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- Prior art keywords
- nitidissima chi
- camellia nitidissima
- camellia
- tissue culture
- substratum
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Abstract
The present invention uses improved MS culture medium as basis, and adds the active carbon, indole-3-acetic acid, 3-indolebutyric acid and 6-benzylaminopurine as auxiliary agent to make tissue cultivation of two key stages of induced growth of bunch bud and seedling-strengthening and rooting of camllia chrysantha. The invention can be used for implementing industrial quick seedling cultivation.
Description
One, technical field
What the present invention relates to is Camellia nitidissima Chi tissue culture fast-propagation substratum, especially for the tissue culture fast-propagation substratum of the growth of Camellia nitidissima Chi inducing clumping bud, two critical stages of strengthening seedling and rooting.
Two, background technology
Camellia nitidissima Chi (C.chysantha) is Theaceae Camellia nitidissima Chi group (Camellia sect.nitidisimaChang) plant, and the kind and the mutation of record at present has 28.Camellia nitidissima Chi is described as " botanic giant panda ", " queen of tea family ", because its ecological condition requirement to existence is high, natural storage is less, mainly is distributed in the southern indivedual areas of China, is rare species.For protecting this rare species, people have carried out scientific researches such as the conservation of resources of Camellia nitidissima Chi and artificial propagation.Seminal propagation is mainly adopted in the artificial propagation of Camellia nitidissima Chi at present, because the natural setting percentage of Camellia nitidissima Chi is very low, causes reproduction speed slower.Camellia nitidissima Chi has higher healthy nutritive value, has some scientific research institutions and enterprise to develop nourishing drink and other products of Camellia nitidissima Chi in recent years, but because wild resource is very limited, also is difficult to commercialize.Have only to solve artificial breeding fast, enlarge the planting number of artificial Camellia nitidissima Chi, set up the planting base that commercial development utilizes, just can make this precious resources obtain the most effective protection and exploitation, realize the true value of its resource.Publication number is 85102805, name is called the patent of invention of culture medium for tissue culture of golden flower tea tree, a kind of improved culture medium based on ER is disclosed, but only on Pingguo Camellia nitidissima Chi (C.pingguooensisD.fang), carried out tentative application, according to the study, it is used in the training of group of Pingguo Camellia nitidissima Chi and truly has effect, but unsatisfactory for the Camellia nitidissima Chi kind effect of the overwhelming majority that accounts for Camellia nitidissima Chi kind and mutation quantity of the tropical rain forest ecology that is distributed in high temperature and rainy.In addition yet there are no the report of other Camellia nitidissima Chi tissue culture fast-propagation substratum.
Three, summary of the invention
Purpose of the present invention provides a kind of Camellia nitidissima Chi tissue culture fast-propagation substratum, is used for the tissue culture fast-propagation of the growth of Camellia nitidissima Chi inducing clumping bud, two critical stages of strengthening seedling and rooting, to overcome the existing existing deficiency of substratum.
For achieving the above object, the present invention has taked such measure: Camellia nitidissima Chi tissue culture fast-propagation substratum is made up of following MS basis component and auxiliary, and described MS basis component refers to:
NH
4NO
3?????????1650mg/L????KNO
3?????????????1900mg/L
Ca(NO
3)
2.4H
2O?300mg/L?????MgSO
4.7H
2O?????370mg/L
KH
2PO
4?????????340mg/L?????KI????????????????0.83mg/L
H3BO
3???????????0.63????????MnSO
4.4H
2O??????22.3mg/L
ZnSO
4.7H
2O?????8.6mg/L?????Na
2Mo
4.H
2O????0.025mg/L
CuSO
4.5H
2O?????0.025mg/L???CoCl
2.6H
2O?????0.0025mg/L
FeSO
4.7H
2O?????27.8mg/L????Na
2.EDTA.7H
2O??37.3mg/L
Inositol 50mg/L nicotinic acid 0.5mg/L
Pyridoxine hydrochloride 0.5mg/L vitamin 0.5mg/L
Glycine 2mg/L sucrose 2-4%
Agar 6.5g/L
Described auxiliary refers to:
Indole-3-acetic acid 0-0.5mg/L 3-indolebutyric acid 0.5-3.0mg/L
6-benzyl aminopurine 0.1-3mg/L gac 0.4-0.8g/L
Above substratum prepares according to a conventional method and inserts in the culturing bottle and use;
Take the present invention of above-mentioned measure, be used for Camellia nitidissima Chi inducing clumping bud and growth, the tissue culture of two critical stages of strengthening seedling and rooting.Tissue culture fast-propagation applicable to most of Camellia nitidissima Chi kind and mutation.Make this first-grade state protection plant of Camellia nitidissima Chi realize rapid propagation in factory, more effectively protect and development and use become possibility.In use can grow body to the young stem of multiple Camellia nitidissima Chi outward and be seeded in the present invention, the 30-45 major part can be induced and be formed the bud of growing thickly, and general inductivity can reach 75-90%.Adjust the prescription consumption on this basis, continue succeeding transfer culture, the bud of growing thickly can be bred in a large number, and 45 days subcultures propagation multiple once is on average in the 3.3-3.7 scope.The bud of growing thickly behind the subculture is transferred among the present invention who adjusts prescription 15-20 days root of hairs in a large number, but 30 days hardening bottle outlets.Go out root rate about 95%.
Four, embodiment
Embodiment 1
Prescription:
NH
4NO
3????????1650mg/L?????KNO
3??????????????1900mg/L
Ca(NO
3)
2.4H
2O300mg/L??????MgSO
4.7H
2O??????370mg/L
KH
2PO
4????????340mg/L??????KI?????????????????0.83mg/L
H3BO
3??????????0.63?????????MnSO
4.4H
2O???????22.3mg/L
ZnSO
4.7H
2O????8.6mg/L??????Na
2Mo
4.H
2O?????0.025mg/L
CuSO
4.5H
2O????0.025mg/L????CoCl
2.6H
2O??????0.0025mg/L
FeSO
4.7H
2O????27.8mg/L?????Na
2.EDTA.7H
2O???37.3mg/L
Inositol 50mg/L nicotinic acid 0.5mg/L
Pyridoxine hydrochloride 0.5mg/L vitamin 0.5mg/L
Glycine 2mg/L sucrose 2-4%
Agar 6.5g/L CaCl
22H
2O
Indole-3-acetic acid 0.5mg/L 3-indolebutyric acid 0.5-mg/L
6-benzyl aminopurine 3mg/L gac 0.4g/L
Above-mentioned prescription prepares according to a conventional method and inserts in the culturing bottle, the young stem of multiple Camellia nitidissima Chi is grown body outward be seeded in the present invention and cultivate, and the result is as follows:
| The Camellia nitidissima Chi kind | Inductivity (%) | 45 days propagation multiples |
| Camellia nitidissima Chi (Golden Camellia) | ?????93.6 | ?????3.46 |
| Show lode gold jasmine tea (Nervose Yellow Camellia) | ?????91.5 | ?????3.37 |
| Concave veins Camellia nitidissima Chi (Impress-nerved Yellow Camellia) | ?????95.8 | ?????3.46 |
| Fruitlet Camellia nitidissima Chi (Smallfruit Yellow Camellia) | ?????79.2 | ?????3.44 |
| Long column Camellia nitidissima Chi (Lonstyle Yellow Cmallia) | ?????83.6 | ?????3.53 |
| Slender lobule Camellia nitidissima Chi (Thinleaf Ywellow Camellia) | ?????84.1 | ?????3.68 |
| Straight veins Camellia nitidissima Chi (Patentvein Yellow Camellia) | ?????87.6 | ?????3.54 |
| Dongxing Camellia nitidissima Chi (Dongxing Yellow Camellia) | ?????87.3 | ?????3.23 |
| Hair seed Camellia nitidissima Chi (Downnyseed Yellow Camellia) | ?????76.4 | ?????3.54 |
| Pingguo Camellia nitidissima Chi (Pingguo Yellow Camellia) | ?????78.3 | ?????3.47 |
| Valvula Camellia nitidissima Chi (Smallpetal Yellow Camellia) | ?????78.8 | ?????3.28 |
Embodiment 2:
It is as follows to adjust prescription:
NH
4NO
3?????????1650mg/L?????????????KNO
3?????????????1900mg/L
Ca(NO
3)
2.4H
2O?300mg/L??????????????MgSO
4.7H
2O?????370mg/L
KH
2PO
4?????????340mg/L??????????????KI????????????????0.83mg/L
H3BO
3????????????0.63????????????????MnSO
4.4H
2O??????22.3mg/L
ZnSO
4.7H
2O??????8.6mg/L?????????????Na
2Mo
4.H
2O????0.025mg/L
CuSO
4.5H
2O??????0.025mg/L???????????CoCl
2.6H
2O?????0.0025mg/L
FeSO
4.7H
2O??????27.8mg/L????????????Na
2.EDTA.7H
2O??37.3mg/L
Inositol 50mg/L nicotinic acid 0.5mg/L
Pyridoxine hydrochloride 0.5mg/L vitamin 0.5mg/L
Glycine 2mg/L sucrose 2-4%
Agar 6.5g/L
3-indolebutyric acid 3mg/L
Gac 0.8g/L 6-benzyl aminopurine 0.1mg/L
Above-mentioned prescription prepares according to a conventional method and inserts in the culturing bottle, the bud of growing thickly behind the subculture is transferred among the present invention cultivated, and the result is as follows:
| The Camellia nitidissima Chi kind | Rooting of vitro seedling rate (%) | Transplanting success (%) |
| Camellia nitidissima Chi (Golden Camellia) | ?????98.6 | ?????87.85 |
| Show lode gold jasmine tea (Nervose Yellow Camellia) | ?????96.7 | ?????89.4 |
| Concave veins Camellia nitidissima Chi (Impress-nerved Yellow Camellia) | ?????92.1 | ?????87.7 |
| Fruitlet Camellia nitidissima Chi (Smallfruit Yellow Camellla) | ?????94.3 | ?????83.6 |
| Long column Camellia nitidissima Chi (Lonstyle Yellow Cmallia) | ?????95.8 | ?????82.6 |
| Slender lobule Camellia nitidissima Chi (Thinleaf Ywellow Camellia) | ?????92.8 | ?????85.6 |
| Straight veins Camellia nitidissima Chi (Patentvein Yellow Camellia) | ?????96.2 | ?????87.1 |
| Dongxing Camellia nitidissima Chi (Dongxing Yellow Camellia) | ?????91.5 | ?????85.5 |
| Hair seed Camellia nitidissima Chi (Downnyseed Yellow Camellia) | ?????97.7 | ?????87.5 |
| Pingguo Camellia nitidissima Chi (Pingguo Yellow Camellia) | ?????97.3 | ?????83..8 |
| Valvula Camellia nitidissima Chi (Smallpetal Yellow Camellia) | ?????95.2 | ?????84.9 |
Claims (2)
1, Camellia nitidissima Chi tissue culture fast-propagation substratum with the ordinary method preparation, has comprised following MS basis component:
NH
4NO
3?????????1650mg/L?????KNO
3????????????1900mg/L
Ca(NO
3)
2.4H
2O?300mg/L??????MgSO
4.7H
2O?????370mg/L
KH
2PO
4?????????340mg/L??????KI???????????????0.83mg/L
H
3BO
3??????????0.63?????????MnSO
4.4H
2O?????22.3mg/L
ZnSO
4.7H
2O?????8.6mg/L??????Na
2Mo
4.H
2O????0.025mg/L
CuSO
4.5H
2O?????0.025mg/L????CoCl
2.6H
2O?????0.0025mg/L
FeSO
4.7H
2O?????27.8mg/L?????Na
2.EDTA.7H
2O??37.3mg/L
Inositol 50mg/L nicotinic acid 0.5mg/L
Pyridoxine hydrochloride 0.5mg/L vitamin 0.5mg/L
Glycine 2mg/L sucrose 2-4%
Agar 6.5g/L;
It is characterized in that described substratum has also comprised auxiliary:
Indole-3-acetic acid 0-0.5mg/L 3-indolebutyric acid 0.5-3.0mg/L
6-benzyl aminopurine 0.1-3mg/L gac 0.4-0.8g/L
2, the purposes of Camellia nitidissima Chi tissue culture fast-propagation substratum as claimed in claim 1 is characterized in that described substratum is used for Camellia nitidissima Chi inducing clumping bud, growth, the tissue culture of two critical stages of strengthening seedling and rooting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03124233 CN1460713A (en) | 2003-04-29 | 2003-04-29 | Camellia chrysantha culture medium for tissue culture and quick breeding |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03124233 CN1460713A (en) | 2003-04-29 | 2003-04-29 | Camellia chrysantha culture medium for tissue culture and quick breeding |
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| Publication Number | Publication Date |
|---|---|
| CN1460713A true CN1460713A (en) | 2003-12-10 |
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ID=29591067
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03124233 Pending CN1460713A (en) | 2003-04-29 | 2003-04-29 | Camellia chrysantha culture medium for tissue culture and quick breeding |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100482067C (en) * | 2006-12-18 | 2009-04-29 | 江苏阳光生态农林开发股份有限公司 | Fast tissue culture propagation process for azalea and camellia |
| CN102823498A (en) * | 2012-09-20 | 2012-12-19 | 重庆文理学院 | Culture medium for subculture multiplication of tissue cultured seedlings of red-flesh kiwifruits |
| CN102972291A (en) * | 2012-11-23 | 2013-03-20 | 广西壮族自治区林业科学研究院 | Tissue culture and propagation method and inductive culture mediums for Chongzuo camellia nitidissima |
| CN103168579A (en) * | 2011-12-23 | 2013-06-26 | 何寒 | Technology assisting golden camellia raising seedlings by cutting in rapidly rooting |
| CN104285813A (en) * | 2014-10-27 | 2015-01-21 | 南宁市金花茶公园 | Camellia chrysantha tissue culture propagation method |
| CN104521753A (en) * | 2014-12-11 | 2015-04-22 | 柳州博泽科技有限公司 | Rapid propagation method of camellia nitidissima |
| CN104663433A (en) * | 2014-12-11 | 2015-06-03 | 柳州博泽科技有限公司 | Rapid tissue culture method for golden camellia |
| CN106857255A (en) * | 2017-02-15 | 2017-06-20 | 钦州市林业科学研究所 | A kind of culture medium of dragon fruit tissue cultures |
| CN109105233A (en) * | 2018-08-08 | 2019-01-01 | 山东省果树研究所 | The copper coin tree seedling fostering method and culture medium quickly bred based on micro cuttage |
| CN110583269A (en) * | 2019-10-22 | 2019-12-20 | 海南大学 | Production method of scion for grafting camellia oleifera in Hainan region |
-
2003
- 2003-04-29 CN CN 03124233 patent/CN1460713A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100482067C (en) * | 2006-12-18 | 2009-04-29 | 江苏阳光生态农林开发股份有限公司 | Fast tissue culture propagation process for azalea and camellia |
| CN103168579A (en) * | 2011-12-23 | 2013-06-26 | 何寒 | Technology assisting golden camellia raising seedlings by cutting in rapidly rooting |
| CN102823498A (en) * | 2012-09-20 | 2012-12-19 | 重庆文理学院 | Culture medium for subculture multiplication of tissue cultured seedlings of red-flesh kiwifruits |
| CN102972291A (en) * | 2012-11-23 | 2013-03-20 | 广西壮族自治区林业科学研究院 | Tissue culture and propagation method and inductive culture mediums for Chongzuo camellia nitidissima |
| CN104285813A (en) * | 2014-10-27 | 2015-01-21 | 南宁市金花茶公园 | Camellia chrysantha tissue culture propagation method |
| CN104521753A (en) * | 2014-12-11 | 2015-04-22 | 柳州博泽科技有限公司 | Rapid propagation method of camellia nitidissima |
| CN104663433A (en) * | 2014-12-11 | 2015-06-03 | 柳州博泽科技有限公司 | Rapid tissue culture method for golden camellia |
| CN106857255A (en) * | 2017-02-15 | 2017-06-20 | 钦州市林业科学研究所 | A kind of culture medium of dragon fruit tissue cultures |
| CN109105233A (en) * | 2018-08-08 | 2019-01-01 | 山东省果树研究所 | The copper coin tree seedling fostering method and culture medium quickly bred based on micro cuttage |
| CN110583269A (en) * | 2019-10-22 | 2019-12-20 | 海南大学 | Production method of scion for grafting camellia oleifera in Hainan region |
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