CN1458164A - Purifying technology for lipid bufalin genin and cino-bufagin in fresh senso - Google Patents
Purifying technology for lipid bufalin genin and cino-bufagin in fresh senso Download PDFInfo
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- CN1458164A CN1458164A CN 03129091 CN03129091A CN1458164A CN 1458164 A CN1458164 A CN 1458164A CN 03129091 CN03129091 CN 03129091 CN 03129091 A CN03129091 A CN 03129091A CN 1458164 A CN1458164 A CN 1458164A
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- Prior art keywords
- toads
- dried venom
- respigon
- cinobufagin
- bright
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Abstract
The present invention discloses the technology of purifying lipid bufogenin and cino-bufogenin in fresh toad cake. The purifying process includes grinding fresh toad cake, adding organic solvent through stirring to obtain toad cake solution, standing, filtering to obtain filtrate, concentrating and drying to obtain the product. The toad cake powder product has relatively high lipid bufogenin and cino-bufogenin content.
Description
Technical field
The present invention relates to a kind of technology of from the bright dried venom of toads, extracting effective ingredient, the purification techniques of Respigon and Cinobufagin in specifically a kind of bright dried venom of toads.
Background technology
Effective ingredient Respigon in the bright dried venom of toads and Cinobufagin are a kind of important source material in the pharmacy, at present, along no effective ways purify out from the bright dried venom of toads purity higher Respigon and Cinobufagin.
Summary of the invention
The technical problem to be solved in the present invention is at above-mentioned deficiency, works out the purification techniques of Respigon and Cinobufagin in a kind of bright dried venom of toads.
Technical scheme of the present invention is: its method of purification is: after the bright dried venom of toads is ground, add organic solvent and stirring, obtain dried venom of toads solution, static then placement, to filter through the dried venom of toads solution of static placement at last, and a filtered liquid that obtains is concentrated, dry, just can make to contain Respigon and the higher dried venom of toads powder product of Cinobufagin amount.
Advantage of the present invention is that the content of Respigon and Cinobufagin of purifying in the dried venom of toads powder product obtain is higher, and this kind dried venom of toads powder product can directly use as pharmaceutical raw material.
Embodiment
The purification techniques of Respigon and Cinobufagin in the bright dried venom of toads of the present invention, its method of purification is: after the bright dried venom of toads of 50 grams is ground, add purity and be 500 milliliters of the organic solvent ethanol of 70%-90%, and stir, obtain dried venom of toads solution, static then placement 2-3 hour, to filter through the dried venom of toads solution of static placement again, then the filtered liquid that obtains is carried out distilled water with distillation method, remove ethanol, and after freeze-drying, just make and contain Respigon and the higher dried venom of toads powder product of Cinobufagin amount.
The above-mentioned organic solvent ethanol that adds also can use ethyl acetate or chloroform or methylene dichloride to replace.Also can be 3 to 8 hours the static storage period of dried venom of toads solution.
Claims (5)
1, the purification techniques of Respigon and Cinobufagin in a kind of bright dried venom of toads, its method of purification is: after the bright dried venom of toads is ground, add organic solvent and stirring, obtain dried venom of toads solution, static then placement, to filter through the dried venom of toads solution of static placement at last, and the filtered liquid that obtains concentrate, dry, just can make and contain Respigon and the higher dried venom of toads powder product of Cinobufagin amount.
2, the purification art of Respigon and Cinobufagin in the bright dried venom of toads according to claim 1 is characterized in that in the bright dried venoms of toads of per 50 grams about 500 milliliters of the organic solvent that adds.
3, the purification art of Respigon and Cinobufagin in the bright dried venom of toads according to claim 1 is characterized in that the organic solvent that is added can be ethanol, also can be ethyl acetate or chloroform.
4, the purification art of Respigon and Cinobufagin in the bright dried venom of toads according to claim 1, the time that it is characterized in that the static placement of dried venom of toads solution is 2 to 8 hours.
5, the purification art of Respigon and Cinobufagin in the bright dried venom of toads according to claim 1 is characterized in that the concentrating of filtered liquid, drying process are: remove organic solvent in the filtered liquid by the distillation mode earlier, then remaining thing freeze-drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03129091 CN1458164A (en) | 2003-06-05 | 2003-06-05 | Purifying technology for lipid bufalin genin and cino-bufagin in fresh senso |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03129091 CN1458164A (en) | 2003-06-05 | 2003-06-05 | Purifying technology for lipid bufalin genin and cino-bufagin in fresh senso |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1458164A true CN1458164A (en) | 2003-11-26 |
Family
ID=29430490
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03129091 Pending CN1458164A (en) | 2003-06-05 | 2003-06-05 | Purifying technology for lipid bufalin genin and cino-bufagin in fresh senso |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1458164A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1302808C (en) * | 2005-11-03 | 2007-03-07 | 中国人民解放军第二军医大学 | Nanometer granule prepn of cinobufagin toxoprotein and its prepn process |
| CN101176739B (en) * | 2006-11-08 | 2011-08-03 | 山东绿叶制药有限公司 | Toadpoison ligand extract as well as preparation method and application thereof |
| CN102247335A (en) * | 2011-06-02 | 2011-11-23 | 江苏省中医药研究院 | Bufalin dry powder inhalant as well as preparation method and application thereof |
| CN102302475A (en) * | 2011-06-02 | 2012-01-04 | 江苏省中医药研究院 | Resibufogenin dry powder inhalant and preparation method and application thereof |
| CN102302477A (en) * | 2011-06-02 | 2012-01-04 | 张振海 | Cinobufagin dry power inhaler and preparation method and application thereof |
| CN104784217A (en) * | 2015-05-08 | 2015-07-22 | 广西大学 | Enteric coated secretio bufonis micropill premix and preparation method thereof |
-
2003
- 2003-06-05 CN CN 03129091 patent/CN1458164A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1302808C (en) * | 2005-11-03 | 2007-03-07 | 中国人民解放军第二军医大学 | Nanometer granule prepn of cinobufagin toxoprotein and its prepn process |
| CN101176739B (en) * | 2006-11-08 | 2011-08-03 | 山东绿叶制药有限公司 | Toadpoison ligand extract as well as preparation method and application thereof |
| CN102247335A (en) * | 2011-06-02 | 2011-11-23 | 江苏省中医药研究院 | Bufalin dry powder inhalant as well as preparation method and application thereof |
| CN102302475A (en) * | 2011-06-02 | 2012-01-04 | 江苏省中医药研究院 | Resibufogenin dry powder inhalant and preparation method and application thereof |
| CN102302477A (en) * | 2011-06-02 | 2012-01-04 | 张振海 | Cinobufagin dry power inhaler and preparation method and application thereof |
| CN102302477B (en) * | 2011-06-02 | 2013-08-28 | 张振海 | Cinobufagin dry power inhaler and preparation method and application thereof |
| CN104784217A (en) * | 2015-05-08 | 2015-07-22 | 广西大学 | Enteric coated secretio bufonis micropill premix and preparation method thereof |
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| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |