CN1455244A - Method of semi-quantitative rapid detecting formaldehyde content in aquatic product - Google Patents
Method of semi-quantitative rapid detecting formaldehyde content in aquatic product Download PDFInfo
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- CN1455244A CN1455244A CN 02110342 CN02110342A CN1455244A CN 1455244 A CN1455244 A CN 1455244A CN 02110342 CN02110342 CN 02110342 CN 02110342 A CN02110342 A CN 02110342A CN 1455244 A CN1455244 A CN 1455244A
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Abstract
The sodium hydroxide solution of phloroglucinol is as the detection liquid. The concentration of phloroglucinol is 1% and the concentration of sodium hydroxide is 12%. Several portions of formalin with the concentration from 0-500 ppm are utilized as the standard substances. When detection of formalin ingredient is carried out, the detection liquid drops to the samples to be tested and the several standard substances. With the color being changed, observing the changer color making the qualitative or semi quantitative determination. The color of the sample to be tested is compared with the colors of standard substances with different grads. If the color of the sample tested is between the colors of standard substances with adjacent concentration values, then the formalin concentration of the sample to be tested is between the products from the concentration and the diluent times.
Description
Technical field
The present invention relates to the method for content of formaldehyde in a kind of sxemiquantitative fast detecting aquatic products.
Background technology
Formaldehyde is a kind of colourless gas with penetrating odor, soluble in water, even also having, rare formalin make cytoplasmic protein that irreversible solidifying be taken place, suppress cell function even kill cell, can damage liver, kidney and nervous centralis, international cancer research institute (IARC) classifies formaldehyde as one of suspicious carcinogenic substance.The traditional detection method of formaldehyde is higher to checker's technical requirement, and the quality of very difficult guarantee reagent, and time-consuming again and effort can not satisfy industrial and commercial administration department in that the market of farm produce etc. is on-the-spot is sensitive, easy in detecting, objective demand fast.
Summary of the invention
The objective of the invention is at the above-mentioned deficiency of prior art and propose a kind of energy quick, easy, detect the method for content of formaldehyde in the aquatic products delicately, be used for that formaldehyde to aquatic products and goods thereof carries out fast, qualitative or half-quantitative detection easily.
The technical solution adopted for the present invention to solve the technical problems is: do to detect liquid with the sodium hydroxide solution of phloroglucin, the concentration of phloroglucin is 1%, and concentration sodium hydroxide is 12%; With concentration is that the formalin of 0-500ppm is made standard items; When making the formaldehyde composition detection, test fluid is splashed into test sample treating fluid and standard items, treat to observe change color after its variable color, and carry out result's qualitative or sxemiquantitative judgement; The sxemiquantitative judgement is carried out as follows: the test sample after relatively developing the color and the color of different gradient standard items, if the test sample color between the standard items color of adjacent certain two concentration, illustrates that then the contained concentration of formaldehyde of this sample is between the product of this two standard items concentration and extension rate.
When making the formaldehyde composition test at the scene, will detect the sealing of liquid and standard items and be kept in plastic bottle or the vial and some blank porous plates, disposable sample cup and disposable calibrated pipet are used.
The present invention has following beneficial effect: (1) is accurately sensitive.As on-the-spot qualitative detection formaldehyde composition, the inventive method has good confidence level, and the lowest detection limit is 20ppm (content of formaldehyde is 100-1000ppm in the aquatic products that generally exceed standard).(2) fast convenient.The present invention qualitatively judges by change in color, and the testing staff need not provide any detection apparatus and equipment for oneself when detecting, and obtains a result in 10-30 minute.(3) cheap.The method that the present invention adopts need not be equipped with any instrument and medicine, and the sample cost is low.(4) steady quality.
Embodiment
The present invention is further described below in conjunction with a preferred embodiment.
After chopping about detected sample 5 gram or grinding, take by weighing about 1/3 glass in numbered sample cup, add the about 10ml of distilled water (not being as the criterion) immersion about 30 minutes just to have sample, during minimum concussion 2 times.
From left to right add 250ul distilled water (blank), 250ul standard items (about 5) and some duplicate samples soak solutions (each sample suggestion adds 2 holes, and every hole 250ul gets final product with disposable calibrated pipet absorption) at blank porous plate successively.
Respectively drip 100ul successively and detect liquid on two holes on " blank " of porous plate and the standard items, the detection hole of each sample also drips two (100ul) and detects liquid.
Observe color reaction, " blank " is colourless, and " standard control " be orange red, and the expression success of the test can be carried out the result and judge.It is orange red that color is, and expression detects formaldehyde, and content is higher; Color is light red, and expression detects formaldehyde, but content is lower; The color no change, expression does not detect formaldehyde.
Carry out according to following steps when carrying out the sxemiquantitative judgement: the test sample after relatively developing the color and the color of different gradient standard items, if the test sample color between the standard items color of adjacent certain two concentration, illustrates that then the contained concentration of formaldehyde of this sample is between this two standard items concentration and extension rate 2 products.
Claims (2)
1, the method for content of formaldehyde in a kind of sxemiquantitative fast detecting aquatic products is characterized in that: do to detect liquid with the sodium hydroxide solution of phloroglucin, the concentration of phloroglucin is 1%, and concentration sodium hydroxide is 12%; With some parts of concentration is that the formalin of 0-500ppm is made standard items; When making the formaldehyde composition detection, test fluid is splashed in test sample treating fluid and the some parts of standard items, treat to observe change color after its variable color, and the qualitative or sxemiquantitative of carrying out the result is judged; The sxemiquantitative judgement is carried out as follows: the test sample after relatively developing the color and the color of different gradient standard items, if the test sample color is between the standard items color of adjacent certain two concentration, the concentration that the contained formaldehyde of this sample then is described is between the product of this two standard items concentration and extension rate.
2, the method for content of formaldehyde in the fast detecting aquatic products according to claim 1, when it is characterized in that making the formaldehyde composition test at the scene, to detect the sealing of liquid and standard items is kept in plastic bottle or the vial and some blank porous plates, disposable sample cup and disposable calibrated pipet are used.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02110342 CN1455244A (en) | 2002-05-02 | 2002-05-02 | Method of semi-quantitative rapid detecting formaldehyde content in aquatic product |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02110342 CN1455244A (en) | 2002-05-02 | 2002-05-02 | Method of semi-quantitative rapid detecting formaldehyde content in aquatic product |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1455244A true CN1455244A (en) | 2003-11-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02110342 Pending CN1455244A (en) | 2002-05-02 | 2002-05-02 | Method of semi-quantitative rapid detecting formaldehyde content in aquatic product |
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| CN (1) | CN1455244A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010127765A1 (en) * | 2009-05-05 | 2010-11-11 | Merck Patent Gmbh | Agent and method for determining aldehyde content |
| CN102998304A (en) * | 2012-10-18 | 2013-03-27 | 南开大学 | Method for qualitatively and semi-quantitatively determining formaldehyde in aqueous solution |
| CN103616380A (en) * | 2013-12-03 | 2014-03-05 | 广东中测食品化妆品安全评价中心有限公司 | Reagent and method for rapidly detecting formaldehyde in foods |
| CN105445260A (en) * | 2015-11-16 | 2016-03-30 | 渤海大学 | Detection method for formaldehyde content in Spanish mackerel |
| CN110118770A (en) * | 2019-04-19 | 2019-08-13 | 广州安诺科技股份有限公司 | It is a kind of for detecting the reagent of sodium formaldehyde sulfoxylate, kit and production technology in food |
-
2002
- 2002-05-02 CN CN 02110342 patent/CN1455244A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010127765A1 (en) * | 2009-05-05 | 2010-11-11 | Merck Patent Gmbh | Agent and method for determining aldehyde content |
| CN102998304A (en) * | 2012-10-18 | 2013-03-27 | 南开大学 | Method for qualitatively and semi-quantitatively determining formaldehyde in aqueous solution |
| CN103616380A (en) * | 2013-12-03 | 2014-03-05 | 广东中测食品化妆品安全评价中心有限公司 | Reagent and method for rapidly detecting formaldehyde in foods |
| CN105445260A (en) * | 2015-11-16 | 2016-03-30 | 渤海大学 | Detection method for formaldehyde content in Spanish mackerel |
| CN105445260B (en) * | 2015-11-16 | 2018-10-30 | 渤海大学 | The detection method of content of formaldehyde in a kind of Spanish mackerel |
| CN110118770A (en) * | 2019-04-19 | 2019-08-13 | 广州安诺科技股份有限公司 | It is a kind of for detecting the reagent of sodium formaldehyde sulfoxylate, kit and production technology in food |
| CN110118770B (en) * | 2019-04-19 | 2022-06-21 | 广州安诺科技股份有限公司 | Reagent and kit for detecting sodium formaldehyde sulfoxylate in food and production process |
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |