CN1454255A - Method for the preparation of stable and reusable biosensing granules - Google Patents
Method for the preparation of stable and reusable biosensing granules Download PDFInfo
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- CN1454255A CN1454255A CN00819852.7A CN00819852A CN1454255A CN 1454255 A CN1454255 A CN 1454255A CN 00819852 A CN00819852 A CN 00819852A CN 1454255 A CN1454255 A CN 1454255A
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- 238000000034 method Methods 0.000 title claims abstract description 48
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 239000008187 granular material Substances 0.000 title abstract 6
- 229920005615 natural polymer Polymers 0.000 claims abstract description 7
- 230000008569 process Effects 0.000 claims abstract description 5
- 239000002245 particle Substances 0.000 claims description 75
- 244000005700 microbiome Species 0.000 claims description 53
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 30
- 239000001301 oxygen Substances 0.000 claims description 30
- 229910052760 oxygen Inorganic materials 0.000 claims description 30
- 239000000243 solution Substances 0.000 claims description 22
- 239000011324 bead Substances 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 239000000126 substance Substances 0.000 claims description 16
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 14
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 14
- 239000002351 wastewater Substances 0.000 claims description 14
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 12
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 9
- 239000008103 glucose Substances 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 8
- 238000004065 wastewater treatment Methods 0.000 claims description 8
- 229910019142 PO4 Inorganic materials 0.000 claims description 7
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 7
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 7
- 235000019270 ammonium chloride Nutrition 0.000 claims description 7
- 229940041514 candida albicans extract Drugs 0.000 claims description 7
- 239000004202 carbamide Substances 0.000 claims description 7
- 238000005119 centrifugation Methods 0.000 claims description 7
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 7
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 7
- 239000010452 phosphate Substances 0.000 claims description 7
- 239000011591 potassium Substances 0.000 claims description 7
- 229910052700 potassium Inorganic materials 0.000 claims description 7
- 239000000523 sample Substances 0.000 claims description 7
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 7
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 7
- 239000007787 solid Substances 0.000 claims description 7
- 108010046845 tryptones Proteins 0.000 claims description 7
- 239000012138 yeast extract Substances 0.000 claims description 7
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 claims description 5
- 235000010413 sodium alginate Nutrition 0.000 claims description 5
- 239000000661 sodium alginate Substances 0.000 claims description 5
- 229940005550 sodium alginate Drugs 0.000 claims description 5
- 230000004913 activation Effects 0.000 claims description 4
- 230000002045 lasting effect Effects 0.000 claims description 4
- 230000036284 oxygen consumption Effects 0.000 claims description 4
- 230000036571 hydration Effects 0.000 claims description 3
- 238000006703 hydration reaction Methods 0.000 claims description 3
- 238000011218 seed culture Methods 0.000 claims description 3
- 230000018044 dehydration Effects 0.000 claims description 2
- 238000006297 dehydration reaction Methods 0.000 claims description 2
- 230000008021 deposition Effects 0.000 claims description 2
- 235000015097 nutrients Nutrition 0.000 claims description 2
- 239000000725 suspension Substances 0.000 claims description 2
- 230000000813 microbial effect Effects 0.000 abstract 3
- 239000002002 slurry Substances 0.000 description 7
- 238000001514 detection method Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 238000010561 standard procedure Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 239000003295 industrial effluent Substances 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- APRRQJCCBSJQOQ-UHFFFAOYSA-N 4-amino-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound OS(=O)(=O)C1=CC(O)=C2C(N)=CC(S(O)(=O)=O)=CC2=C1 APRRQJCCBSJQOQ-UHFFFAOYSA-N 0.000 description 3
- 239000001110 calcium chloride Substances 0.000 description 3
- 229910001628 calcium chloride Inorganic materials 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 239000002957 persistent organic pollutant Substances 0.000 description 3
- 239000004753 textile Substances 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 238000010170 biological method Methods 0.000 description 2
- 238000001311 chemical methods and process Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 229930014626 natural product Natural products 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 238000013022 venting Methods 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 238000009529 body temperature measurement Methods 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 235000011089 carbon dioxide Nutrition 0.000 description 1
- JYYOBHFYCIDXHH-UHFFFAOYSA-N carbonic acid;hydrate Chemical compound O.OC(O)=O JYYOBHFYCIDXHH-UHFFFAOYSA-N 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000002848 electrochemical method Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 239000003673 groundwater Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000000266 injurious effect Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 229910017464 nitrogen compound Inorganic materials 0.000 description 1
- 150000002830 nitrogen compounds Chemical class 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000007669 thermal treatment Methods 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/1806—Biological oxygen demand [BOD] or chemical oxygen demand [COD]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/186—Water using one or more living organisms, e.g. a fish
- G01N33/1866—Water using one or more living organisms, e.g. a fish using microorganisms
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Food Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- General Physics & Mathematics (AREA)
- Dispersion Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Emergency Medicine (AREA)
- Molecular Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Biological Treatment Of Waste Water (AREA)
Abstract
The invention relates to a process for the preparation of stable and reusable biosensing granules useful in the assessment of biodegradability of effluents. The granules of the invention are prepared by developing active aerobic microbial consortia in synthetic medium, separating the active aerobic microbial consortia, immobilising the said microbial consortia using a natural polymer to form biosensing granules, dehydrating the immobilised biosensing granules to obtain stable biosensing granules having a moisture content of 5-30%.
Description
Invention field
The present invention relates to a kind of reusable bio-sensing particulate preparation method that stablizes of the biological treatment performance that can be used to assess effluent.
Background of invention
The bio-sensing particle that the present invention develops will help any effluent at scene is carried out fast characterizing.And bio-sensing particle of the present invention can be repeatedly used, and only takies manpower seldom.In addition, these biological particless are favourable to environment in fact, the saving cost, and do not need to introduce any chemical preparations or energy, thereby are easy to operate under the condition in the open air.
By product liquid stream from various Different Industries contains the compound that different environment is not welcome, and they can produce injurious effects to retention basin and groundwater reservoir.Before these effluents of discharging are in the environment, reduce organic pollutant to environmentally acceptable limit, be very important.This requires one to measure organic content in these discharges in advance, and it will help to assess the concentration of pollutent, the design of treatment process and disposable replacement scheme, or the like.Biodegradable organic concentration in the effluent can be determined with the ratio of chemical oxygen demand (COD) (COD) by calculating biological oxygen demand (BOD).Prolonged analysis time, not responsible simulated experiment condition and the toxicity of effluent have limited its decision of selecting the suitable processing method at the scene of using and making subsequently.
The selection that effluent is handled can be divided into following a few class type: physical treatment, chemical treatment, biological treatment and thermal treatment usually.In these above-mentioned types, bioremediation is better than other several method, and this is that as methane, carbonic acid gas and water, they are favourable for environment in fact because it has the possibility that degradable organic pollutant is the compound of simple environment safety.The biological treatment of effluent is by inoculating suitable microorganism pairing (microbialconsortia) and inoculating in anaerobic condition or aerobic condition and realize.Under aerobic conditions, be present in the organic compound generate energy that microorganism pairing in the system utilizes oxygen and effluent.Pollutant levels can be estimated by microorganism activity in the calculating waste water.Therefore, to breathe the microorganism activity of expression, be an index that is characterized in effluent under the different biological treatment types.
Partly, people have done repeatedly and have attempted, and adopt chemical process and biological method, measure organic content in the industrial effluent, in order to characterize these effluents, assess its biodegradability, and are used for selecting suitable treatment process.[Rogers，K.R.and?Williams，L.R.(1995)Trends?Anal.Chem.14；289-294]。The organic content of effluent is by being to represent that with the required oxygen quantity of degradable organic pollutant it can be measured by chemical process or biological method.Article one, being used for assessing the empirical rule of the biological treatment performance rule of effluent, is biological oxygen demand (BOD) and the ratio of chemical oxygen demand (COD) (COD), and it is a mark normally.Low BOD/COD numerical value shows that it is difficult to biological degradation, and high numerical value represents that then refuse is fit to biodegrade.
Biochemical oxygen demand (BOD) mainly is a kind of bioassay method, and the specified requirements that is included in standard is assessed the oxygen that consumes in the simulator down.BOD is in the BOD of 300ml capacity bottle, gets suitable effluent aliquots containig, can take from raw waste water, also can take from the treated effluent of waste water treatment plant, in the presence of seed and substratum, finishes through 5 days in 20 ℃.The time that is used to analyze, not responsible simulated experiment condition and the toxicity of effluent have been offseted its application.In this piece article, COD is arranged, wherein the organic substance in the effluent under sour environment in comparatively high temps by the oxidation of a kind of strong oxidizer institute.This test needed analyze in about 3 hours, and it does not rely on coenocorrelation.But this method has the limitation of himself, as not responsible precision, introduce chemical substance and energy and produce second kind and flow out handling problem, or the like.The BOD/COD characterizing method of this effluent expends time in, and the lab setup that need have particular instrument needs to introduce chemical substance and energy to keep required steady temperature, generally can not be used for field conditions.
Other the waste water characterization technique that is used for biodegradability is a uptake rate of measuring its inorganic nitrogen compound.But these methods will be only applicable to the refuse of some type.And they need specific instrument, to measure various mineral compound in the effluent.Recently, people are carrying out effort, attempt the ventilation characteristic measuring technology with a kind of existing advanced person, mix respirometer, combine with titration technique and carry out, and have obtained limited success.[Vanrolleghem，P.A.and?Spanjers，H.(1998)Wat.Sci.Technol.，37；237-246]。Most other researchists also attempt to develop the biosensor based on microorganism, and it relates to adopts spectrophotometric method or electrochemical method determining microorganism activity.[Bains，W.(1994)Biosensors?Bioelectronics.，9；111-117；Corbisier，P.，Thiry，E.，and?Diels，L.(1996)Environ.Toxicol.Water?Qual.，1l；171-177；Silva，M.J.，and?Wong，J.L.(1995)Bioelectrochem.Bioener.，37；141-148]。The shortcoming of all these technology is the inoculation cycle of length and low initial response, or the like.[Rogers，K.R.and?Koglin，E.N.(1997)inBiosensors?for?Direct?Monitoring?of?Environmental?Pollution?inField，(ed.D.P.Nikolelis，U.J.Krull，J.Wang，and?M.Mascini)Kluwer?Publishers，Boston，335-349]。And, characterize for on-the-spot effluent, adopt these technology not carry out.[Koglin，E.N.And?Williams，L.R.(1994)Trends.Anal.Chem.13；294-299]。Therefore, develop a kind of dependable method, will advance the progress of environmental monitoring significantly with the required oxygen of biodegradation assessment apace.
Goal of the invention
The reusable bio-sensing particulate preparation method that stablizes who the purpose of this invention is to provide a kind of biological treatment performance that can be used to measure effluent.
Another object of the present invention provides a kind of evaluation method of saving the effluent biological treatment performance of cost.
Another purpose of the present invention provides a kind of work box that is used for effectively characterizing under the condition in the open air effluent biological treatment performance.
Another object of the present invention provides a kind of reusable bio-sensing particle that is used to measure the biodegradability of effluent of stablizing.
Another purpose of the present invention provides the information of relevant on-the-spot emission performance.
Another object of the present invention provides a kind of technology of enabling environment, and it does not need chemical substance for the refuse sign of biological treatment.
Another purpose of the present invention provides a kind of method of biological treatment performance of rapid determination effluent.
Summary of the invention
Therefore, the invention provides a kind of reusable bio-sensing particulate preparation method that stablizes of the biodegradability that can be used to assess effluent, described method is included in and cultivates active aerobic microorganism pairing in the synthetic medium, isolate described active aerobic microorganism pairing, adopt the fixing described microorganism pairing of natural polymer to form the bio-sensing particle, described fixed bio-sensing particle was dewatered at 24-32 ℃ of lasting 4-12 hour, was the stable bio-sensing particle of 5-30% to obtain moisture content.
The invention still further relates to a kind of reusable bio-sensing particulate preparation method that stablizes of the biological treatment performance that can be used to assess effluent, it comprises:
I. from raw waste water, waste water treatment plant or activatory mud device, select a kind of seed culture;
Ii. prepare a kind of synthetic medium;
Iii. in a kind of microorganism pairing of described inoculation of medium;
Iv. under the aerobic condition of the about 5ml/min. of air velocity, cultivate described microorganism in about 28 ℃ and match, continue 12-24 hour, perhaps, up to forming in dry weight, the content of mixing liquid suspended solids (MLSS) reaches 14500-15500mg/l;
V. carry out centrifugation by lasting 10-15 minute of suitable rotating speed and about 28 ℃ temperature, isolate described active aerobic microorganism pairing;
Vi. adopt hydration natural polymer solution,, obtain fixed bio-sensing bead by the fixing described microorganism pairing of any known process;
Vii. go out described bio-sensing bead by the described solution separating of decant;
Viii. water thoroughly cleans repeatedly described bead;
Ix. 24-36 ℃ temperature range described bead is dewatered, continue 2-20 hour, obtaining moisture content is the stable bio-sensing particle of 5-30%;
X. by in the aqueous solution of 2-5% (w/v), carrying out 2-10 hour in 28 ℃, activate described stable bio-sensing particle, obtain active stabilate sensitive particles;
Xi. adopt ordinary method from described activated solution, to isolate described active particle.
In one embodiment of the invention, described aerobic microorganism pairing is collected in raw waste water, waste water treatment plant or activatory inflation mud device.
In another embodiment of the invention, used synthetic medium is formed (g/L) by following substances: glucose 29-31; Ammonium chloride 5.5-7.5; Potassium primary phosphate l.5-3.5; Dipotassium hydrogen phosphate 0.5-1.5; Sodium bicarbonate 4.5-5.5; Yeast extract paste 0.5-1.5; Urea 0.3-0.7; With tryptones 0.5-1.5.
In another embodiment of the invention, the pH value of prepared synthetic medium, hydrochloric acid or the 0.1N sodium hydroxide of employing 0.1N are adjusted to about 7.0.
In another embodiment of the invention, the described collection microorganism pairing of about 10% (w/v) is seeded in the described synthetic medium.
In another embodiment of the present invention, described inoculation synthetic medium is to flow through by the air with about 5ml/min. speed to inflate.
In another embodiment of the invention, described substratum is to cultivate 24-32 ℃ temperature.
In another embodiment of the invention, it is to stop after mixing liquid suspended solids (MLSS) reaches 14500-15500mg/l that described active aerobic microorganism paired is cultivated.
In another embodiment of the invention, described active aerobic microorganism pairing is to adopt the ordinary method that is selected from centrifugation, deposition, the described suspension of decant, separates from described liquid nutrient medium.
In another embodiment of the present invention, described isolating active aerobic microorganism pairing is to adopt sodium alginate and the 0.2M calcium chloride solution of 1-3% (w/v) to carry out fixed.
In another embodiment of the invention, the described active aerobic microorganism paired content range that is used for fixing is 3-5% (w/v), to obtain fixed bio-sensing particle.
In another embodiment of the invention, prepared fixed biologically sensitive particles is to cultivate 12-24 hour in 4 ℃ in the 0.2M calcium chloride solution.
In another embodiment of the invention, prepared fixed biologically sensitive particles is separated from described calcium chloride solution by the described aqueous solution of decant.
In another embodiment of the invention, described fixed bio-sensing particle is 2-20 hour dehydration the 24-32 ℃ of time of carrying out, and is the stable bio-sensing particle of 5-30% to obtain moisture content.
In another embodiment of the present invention, described stable bio-sensing particle is in 24-32 ℃ of cultivation 2-10 hour, to obtain active stable bio-sensing particle at 2-5% (w/v) glucose solution.
In another embodiment of the invention, described stable bio-sensing particle is to go out described solution and separate from described activated media by decant.
In another embodiment of the present invention, the remaining dissolved oxygen content of described effluent is, adopts oxygen probe, add content range be the stable bio-sensing particle of the activation of 2-5% (w/v) before and 2-6 hour after measure and carry out.
The invention still further relates to a kind of method that adopts the described bio-sensing particle of claim 1 to be used for assessing the biological treatment performance of effluent, wherein, if the rate of consumption of described activatory bio-sensing particulate dissolved oxygen is greater than 2mg/l, represent that then described effluent can carry out a biological disposal upon to heavens, when described oxygen consumption rate between 1.0-2.0mg/l, can carry out a biological disposal upon, when described oxygen consumption rate is lower than 1.0mg/l, then be that the degree that can carry out a biological disposal upon is low then moderately.
Detailed description of the Invention
Bio-sensing particle of the present invention, mechanicalness is firm, and biologically active can adopt 24-32 ℃ temperature range and assess the biodegradability of effluent in 2-4 hour, and did not need to relate to BOD instrument or COD analysis.
Method of the present invention comprises that preparation is a kind of and stablizes the application that reusable bio-sensing particle and its are used for assessing the biological treatment performance of effluent.Described seed culture is to be selected from raw waste water, waste water treatment plant or activatory mud device.A kind of synthetic medium is formed (in g/L) by following substances: glucose 30.0; Ammonium chloride 6.5; Potassium primary phosphate 2.5; Dipotassium hydrogen phosphate 1.0; Sodium bicarbonate 5.5; Yeast extract paste 1.0; Urea 0.5; With tryptones 1.0, pH is 7.0, it is to carry out preparing in 12-24 hour in 28 ℃ under the venting condition of 5ml/min. at air velocity, perhaps, is to form and preparation up to dry weight that mixing liquid suspended solids (MLSS) reaches 14500-15500mg/l.The active aerobic microorganism pairing that obtains is carried out realizing in 10-15 minute separating in 28 ℃ by being preferably 10000rpm at suitable rotating speed.Described microorganism pairing adopts hydration natural polymer solution to fix with currently known methods, with the responsive bead of the microorganism biological that is fixed.These beads are assigned to by the described solution separating of decant, and the thorough washing of water repeatedly, dewaters 24-36 ℃ temperature range, and the time length is 2-20 hour, obtains having the stabilate sensitive particles that moisture content is 5-30%.
Of the present inventionly stablizing reusable bio-sensing particle, is that diameter is the granular spheroidal particle of 0.3-1.0mm, be a bit darkish in colour, and firm blocky particle, they are insoluble to moisture or organic medium.These particles have the ability of inherent absorption or desorption water molecules.These stable particles can by in 2-5% (w/v) aqueous solution in 28 ℃ to they cultivate obtained in 2-10 hour active stable bio-sensing particle obtain the activation.These active particles are separated from described activated solution by ordinary method.Collect various dissimilar industrial effluents from industrial site, and cultivate, to obtain active stabilate particle with about 1-5% (w/v).Dissolved oxygen in the effluent was measured before adding described activation biological particles, and measured once more after particle adds two hours.
Described active aerobic microorganism paired source is raw waste water or waste water treatment plant or inflation sludge treatment equipment.
The described active aerobic microorganism paired synthetic medium that is used for cultivating collection is formed (in g/L) by following substances: glucose 30.0; Ammonium chloride 6.5; Potassium primary phosphate 2.5; Dipotassium hydrogen phosphate 1.0; Sodium bicarbonate 5.5; Yeast extract paste 1.0; Urea 0.5; With tryptones 1.0, pH is 7.0 ±, it is to carry out preparing in 12-24 hour in 28 ℃ under the venting condition of 5ml/min. at air velocity, perhaps, is to form and preparation up to dry weight that mixing liquid suspended solids (MLSS) reaches 14500-15500mg/l.
Preferably, described bio-sensing particle is that microorganism pairing by mixing described mixing liquid suspended solids (MLSS) 600-8500mg/l becomes a kind of uniform mixture up to it at 2% (w/v) in the natural or synthetic polymers soln and prepares.This slurry adopts a kind of peristaltic pump to be added drop-wise to the curing solution of a kind of 0.2M with the drop form, to make the uniform-dimension particle that diameter is 1.0-1.5mm.These particles are in 4 ℃ of solidify overnight in the 0.2M curing solution, and wash twice with water, then carry out drying in room temperature.
To go out thing treatment plant mainly by the different industrial effluents of chemical industry waste water composition, the textile industry effluent of mainly forming by H-acid with from the effluent of the natural product extraction industry that relates to plant from normal stream, be collected among the bottle that is used for the object of the invention.The effluent of collecting adopts sorting technique, characterizes their chemical oxygen demand (COD) and biological oxygen demand.
Example 1:
Prepare stable bio-sensing particle:
Active aerobic microorganism pairing is to collect from the waste water treatment plant.The preparation synthetic medium is formed (in g/L) by following compositions: glucose 30.0; Ammonium chloride 6.5; Potassium primary phosphate 2.5; Dipotassium hydrogen phosphate 1.0; Sodium bicarbonate 5.5; Yeast extract paste 1.0; Urea 0.5; With tryptones 1.0.The pH of described synthetic medium adopts 0.1N hydrochloric acid or 0.1N sodium hydroxide solution to be adjusted to 7.0.The microorganism pairing of collecting is inoculated in described synthetic medium, and is under the aerobic condition of 5ml/min. at air velocity, cultivates in 28 ℃, and the dry weight that reaches 15500mg/l up to MLSS is formed.The living microorganism pairing was separated by carrying out centrifugation in 28 ℃ at 10000rpm in 10 minutes, and living microorganism pairing slurry adopts the pairing of 4% (w/v) living microorganism and 2% (w/v) sodium alginate soln to prepare in water.This slurry then is added drop-wise in the 0.2M calcium chloride with the drop form, and fixed bio-sensing bead continues to cultivate 18 hours in 4 ℃ in same solution.Described bead is then separated by draining described calcium chloride solution, and the water repeated washing repeatedly.Described fixed bio-sensing bead then dewatered 12 hours in 28 ℃, obtained stable bio-sensing particle.
Example 2:
Active aerobic microorganism pairing is to collect from sewage work.The preparation synthetic medium is formed (in g/L) by following compositions: glucose 30.0; Ammonium chloride 6.5; Potassium primary phosphate 2.5; Dipotassium hydrogen phosphate 1.0; Sodium bicarbonate 5.5; Yeast extract paste 1.0; Urea 0.5; With tryptones 1.0.The pH of described synthetic medium adopts 0.1N hydrochloric acid or 0.1N sodium hydroxide solution to be adjusted to 7.0.The microorganism pairing of collecting is inoculated in described synthetic medium, and is under the aerobic condition of 5ml/min. at air velocity, cultivates in 30 ℃, and the dry weight that reaches 14500mg/1 up to MLSS is formed.The living microorganism pairing is separated by centrifugation.Living microorganism pairing slurry adopts the pairing of 5% (w/v) living microorganism and 4% (w/v) sodium alginate soln to prepare in water.This slurry then is added drop-wise in the 0.2 M calcium chloride with the drop form, and fixed bio-sensing bead continues to cultivate 12 hours in 4 ℃ in same solution.Described bead is then separated by draining described calcium chloride solution, and the water repeated washing repeatedly.Described fixed bio-sensing bead then dewatered 18 hours in 24 ℃, obtained stable bio-sensing particle.
Example 3:
Active aerobic microorganism pairing is to collect from raw waste water.The preparation synthetic medium is formed (in g/L) by following substances: glucose 30.0; Ammonium chloride 6.5; Potassium primary phosphate 2.5; Dipotassium hydrogen phosphate 1.0; Sodium bicarbonate 5.5; Yeast extract paste 1.0; Urea 0.5; With tryptones 1.0.The pH of described synthetic medium adopts 0.1N hydrochloric acid or 0.1N sodium hydroxide solution to be adjusted to 7.0.The microorganism pairing of collecting is inoculated in described synthetic medium, and is under the aerobic condition of 5ml/min. at air velocity, cultivates in 28 ℃, and the dry weight that reaches 15000mg/l up to MLSS is formed.The living microorganism pairing was separated by carrying out centrifugation in 28 ℃ at 5000rpm in 15 minutes, and living microorganism pairing slurry adopts the pairing of 5% (w/v) living microorganism and 1% (w/v) sodium alginate soln to prepare in water.This slurry then is added drop-wise in the 0.2M calcium chloride with the drop form, and fixed bio-sensing bead continues to cultivate 24 hours in 4 ℃ in same solution.Described bead is then separated by draining described calcium chloride solution, and the water repeated washing repeatedly.Described fixed bio-sensing bead then dewatered 18 hours in 30 ℃, obtained stable bio-sensing particle.
Example 4:
Adopt synthetic medium that the bio-sensing particle is characterized
The BOD of described synthetic medium and COD are by adopting by the AmericanPublic Health Association (APHA) (1967) Standard Methods forExamination of Water and Wastewater, Washington, the standard method of DC. defined is measured.Get this synthetic medium 300ml, put in the standard BOD bottle, to wherein adding 0.2g activatory bio-sensing particle (BSG).Adopt dissolved oxygen probe, dissolved oxygen level in this solution of indoor temperature measurement.
| ??S.NO. | ??BOD 5(mg/l) | ?COD(mg/l) | ??BOD 5/COD | Dissolved oxygen/2 hour the BSG (mg/l) that consume |
| ????1 | ????7410 | ????9500 | ????0.780 | ????2.94 |
| ????2 | ????7450 | ????9550 | ????0.780 | ????2.95 |
| ????3 | ????7350 | ????9450 | ????0.777 | ????2.93 |
Example 5:
Adopt the bio-sensing particle that the common effluent of industry is characterized
A kind of effluent is to collect from common effluent treatment plant, and it is made up of various Industrial emissions.According to by the American Public Health Association (APHA) (1967) Standard Methods for Examination of Water and Wastewater, Washington, DC. described method is carried out COD and BOD to described effluent
5Characterize.
In a BOD bottle, put into above-mentioned effluent 300ml, to wherein adding 0.2g activatory bio-sensing particle.Begin to adopt dissolved oxygen probe, under room temperature, and after two hours, measure dissolved oxygen in the described bottle.
| ??S.No. | ??BOD 5(mg/l) | ?COD(mg/l) | ??BOD 5/COD | Dissolved oxygen/2 hour the BSG (mg/l) that consume |
| ????1 | ????6440 | ????10625 | ????0.606 | ????1.47 |
| ????2 | ????6340 | ????10400 | ????0.609 | ????1.46 |
| ????3 | ????6540 | ????10760 | ????0.607 | ????1.48 |
Example 6:
Adopt the bio-sensing particle that the textile industry effluent is characterized
Collect a kind of textile industry effluent, mainly form by H-acid, and according to by APHA (1967) Standard Methods for Examination of Water and Wastewater, Washington, the described method of DC. is carried out COD and BOD to it
5Characterize.In a BOD bottle, put into H-acid effluent 300ml, to wherein adding 0.2g activatory bio-sensing particle.Begin to adopt dissolved oxygen probe, under room temperature, and after two hours, measure dissolved oxygen in the described bottle.
| ??S.No. | ??BOD 5(mg/l) | ?COD(mg/l) | ???BOD 5/COD | Dissolved oxygen/2 hour the BSG (mg/l) that consume |
| ????1 | ????215 | ????766 | ????0.280 | ????0.60 |
| ????2 | ????210 | ????764 | ????0.274 | ????0.59 |
| ????3 | ????220 | ????769 | ????0.286 | ????0.61 |
Example 7:
Adopt the bio-sensing particle that industrial effluent is characterized
Collect a kind of effluent that relates to the natural product extraction industry of plant, and according to by APHA (1967) Standard Methods for Examination of Water andWastewater, Washington, the described method of DC. is to its a plurality of parameters such as COD and BOD
5Analyze.In a BOD bottle, put into the above-mentioned effluent of 300ml, to wherein adding 0.2g activatory bio-sensing particle.Begin to adopt dissolved oxygen probe, under room temperature, and after two hours, measure dissolved oxygen in the described bottle.
| ??S.No. | ??BOD 5(mg/l) | ?COD(mg/l) | ????BOD 5/COD | Dissolved oxygen/2 hour the BSG (mg/l) that consume |
| ????1 | ????7260 | ????16200 | ????0.448 | ????0.84 |
| ????2 | ????7270 | ????16250 | ????0.447 | ????0.82 |
| ????3 | ????7310 | ????16340 | ????0.447 | ????0.86 |
Major advantage of the present invention:
1. the invention provides the method for its biological treatment performance of a kind of fast characterizing effluent.
2. prepared stabilate sensitive particles can for a long time and not can loss of activity at room temperature storage.
3. prepared stabilate particle can be repeatedly used, for detection of the biological treatment performance of effluent.
4. prepared bio-sensing particle can be used among the scene.
5. prepared bio-sensing particle is easy to use, and uses them not need professional and/or person skilled in the art.
6. the bio-sensing particle that makes of the present invention is so that the cost savings of effluent biological treatment Performance Detection.
7. the present invention is by the biological treatment performance of stabilate sensitive particles detection effluent, and it provides a kind of simple and convenient being used for to characterize the technology of effluent biological treatment performance.
8. the present invention is by the biological treatment performance of stabilate sensitive particles detection effluent, and it only needs minimized precautionary measures.
9. the present invention is by the biological treatment performance of stabilate sensitive particles detection effluent, and it can be used to biodegradable organic content in the Fast Evaluation effluent.
10. the present invention is by the biological treatment performance of stabilate sensitive particles detection effluent, and when characterizing effluent, it does not need to introduce in addition any chemical substance.
11. the present invention can be used in the short time a large amount of sample evaluations.
12. any effluent all can adopt stabilate sensitive particles of the present invention to characterize.
Claims (19)
1. a biodegradability that can be used to assess effluent stablizes reusable bio-sensing particulate preparation method, described method is included in and cultivates active aerobic microorganism pairing in the synthetic medium, isolate described active aerobic microorganism pairing, adopt the fixing described microorganism pairing of natural polymer to form the bio-sensing particle, described fixed bio-sensing particle was dewatered at 24-32 ℃ of lasting 4-12 hour, was the stable bio-sensing particle of 5-30% to obtain moisture content.
2. method according to claim 1 comprises:
I. from raw waste water, waste water treatment plant or activatory mud device, select a kind of seed culture;
Ii. prepare a kind of synthetic medium;
Iii. in a kind of microorganism pairing of described inoculation of medium;
Iv. under the aerobic condition of the about 5ml/min. of air velocity, cultivate described microorganism in about 28 ℃ and match, continue 12-24 hour, perhaps, up to forming in dry weight, the content of mixing liquid suspended solids reaches 14500-15500mg/l;
V. carry out centrifugation by lasting 10-15 minute of suitable rotating speed and about 28 ℃ temperature, isolate described active aerobic microorganism pairing;
Vi. adopt hydration natural polymer solution,, obtain fixed bio-sensing bead by the fixing described microorganism pairing of any known process;
Vii. go out described bio-sensing bead by the described solution separating of decant;
Viii. water thoroughly cleans repeatedly described bead;
Ix. 24-36 ℃ temperature range described bead is dewatered, continue 2-20 hour, obtaining moisture content is the stable bio-sensing particle of 5-30%;
X. by in the aqueous solution of 2-5% (w/v), carrying out 2-10 hour in 28 ℃, activate described stable bio-sensing particle, obtain active stabilate sensitive particles;
Xi. adopt ordinary method from described activated solution, to isolate described active particle.
3. method according to claim 1, wherein, described aerobic microorganism pairing is collected in raw waste water, waste water treatment plant or activatory inflation mud device.
4. method according to claim 1, wherein said synthetic medium is formed (g/L) by following substances: glucose 29-31; Ammonium chloride 5.5-7.5; Potassium primary phosphate 1.5-3.5; Dipotassium hydrogen phosphate 0.5-1.5; Sodium bicarbonate 4.5-5.5; Yeast extract paste 0.5-1.5; Urea 0.3-0.7; With tryptones 0.5-1.5.
5. method according to claim 1, wherein, the pH value of prepared synthetic medium adopts hydrochloric acid or the 0.1N sodium hydroxide of 0.1N, is adjusted to about 7.0.
6. method according to claim 1, wherein, the described collection microorganism pairing of about 10% (w/v) is seeded in the described synthetic medium.
7. method according to claim 1, wherein, described inoculation synthetic medium is to flow through by the air with about 5ml/min. speed to inflate.
8. method according to claim 1, wherein, described substratum is to cultivate 24-32 ℃ temperature.
9. method according to claim 1, wherein, it is termination mixing liquid suspended solids (MLSS) reaches 14500-15500mg/l after that described active aerobic microorganism paired is cultivated.
10. method according to claim 1, wherein, described active aerobic microorganism pairing is to adopt the ordinary method that is selected from centrifugation, deposition, the described suspension of decant, separates from described liquid nutrient medium.
11. one kind as method as described in the claim 10, described isolating active aerobic microorganism pairing is to adopt sodium alginate and the 0.2M calcium chloride solution of 1-3% (w/v) to carry out fixed.
12. a method according to claim 1, wherein, the described active aerobic microorganism paired content range that is used for fixing is 3-5% (w/v), to obtain fixed bio-sensing particle.
13. a method according to claim 1, wherein, prepared fixed biologically sensitive particles is to cultivate 12-24 hour in 4 ℃ in the 0.2M calcium chloride solution.
14. a method according to claim 1, wherein, prepared fixed biologically sensitive particles is separated from described calcium chloride solution by the described aqueous solution of decant.
15. a method according to claim 1, wherein, described fixed bio-sensing particle is 2-20 hour dehydration the 24-32 ℃ of time of carrying out, and is the stable bio-sensing particle of 5-30% to obtain moisture content.
16. a method according to claim 1, wherein, described stable bio-sensing particle is to cultivate 2-10 hour in 24-32 ℃ at 2-5% (w/v) glucose solution, to obtain active stable bio-sensing particle.
17. a method according to claim 1, wherein, described stable bio-sensing particle is to go out described solution and separate from described activated media by decant.
18. a method according to claim 1, wherein, the remaining dissolved oxygen content of described effluent is, adopts oxygen probe, add content range be the stable bio-sensing particle of the activation of 2-5% (w/v) before and 2-6 hour after measure and carry out.
19. method that adopts the described bio-sensing particle of claim 1 to be used for assessing the biological treatment performance of effluent, wherein, if the rate of consumption of described activatory bio-sensing particulate dissolved oxygen is greater than 2mg/l, represent that then described effluent can carry out a biological disposal upon to heavens, when described oxygen consumption rate between 1.0-2.0mg/l, can carry out a biological disposal upon, when described oxygen consumption rate is lower than 1.0mg/l, then be that the degree that can carry out a biological disposal upon is low then moderately.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/IN2000/000082 WO2002018563A1 (en) | 2000-08-31 | 2000-08-31 | Method for the preparation of stable and reusable biosensing granules |
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| EP (1) | EP1313847A1 (en) |
| CN (1) | CN1454255A (en) |
| AU (1) | AU2001230484A1 (en) |
| DE (1) | DE10085484B4 (en) |
| WO (1) | WO2002018563A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011022895A1 (en) * | 2009-08-31 | 2011-03-03 | 清华大学 | Rapid measurement method for biochemical oxygen demand (bod) by using saccharomyces cerevisiae as biological recognition elements |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6793822B2 (en) * | 2002-02-22 | 2004-09-21 | Sut Seraya Pte Ltd. | Aerobic biomass granules for waste water treatment |
| WO2006075030A2 (en) * | 2005-01-17 | 2006-07-20 | Universidad Técnica Federico Santa María | Biosensor for determining the biochemical oxygen demand (bod) by respirometry |
| WO2013166611A1 (en) | 2012-05-08 | 2013-11-14 | Granit Technologies S.A. | Method for simultaneous biological removal of nitrogen compounds and xenobiotics of wastewaters |
| CN110902963B (en) * | 2019-12-10 | 2022-06-24 | 九江天赐高新材料有限公司 | Treatment method of alkali-soluble polymer-containing wastewater |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5830537B2 (en) * | 1976-10-13 | 1983-06-29 | 味の素株式会社 | Biochemical oxygen demand measurement method |
| FR2637611B1 (en) * | 1988-10-07 | 1991-10-18 | Bernis Alain | METHOD FOR FIXING MICROORGANISMS ON POLYMER PARTICLES AND PURIFICATION METHOD USING PARTICLES THUS COLONIZED |
| JPH04218373A (en) * | 1990-12-18 | 1992-08-07 | Shikoku Chem Corp | Production of inclusively carrier-immobilized microorganism |
| JPH0673451B2 (en) * | 1992-02-12 | 1994-09-21 | 工業技術院長 | Immobilized microorganism reaction method |
| GB2339435B (en) * | 1998-06-20 | 2003-02-26 | Council Scient Ind Res | A reusable immobilised microbial composition useful as ready-to-use seed inoculum in BOD analysis |
-
2000
- 2000-08-31 WO PCT/IN2000/000082 patent/WO2002018563A1/en active Application Filing
- 2000-08-31 EP EP00990871A patent/EP1313847A1/en not_active Withdrawn
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011022895A1 (en) * | 2009-08-31 | 2011-03-03 | 清华大学 | Rapid measurement method for biochemical oxygen demand (bod) by using saccharomyces cerevisiae as biological recognition elements |
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| AU2001230484A1 (en) | 2002-03-13 |
| DE10085484B4 (en) | 2006-04-27 |
| WO2002018563A1 (en) | 2002-03-07 |
| DE10085484T1 (en) | 2003-07-17 |
| EP1313847A1 (en) | 2003-05-28 |
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