CN1453572A - Detection of medical polyacrylamide hydrogel during its prepn - Google Patents
Detection of medical polyacrylamide hydrogel during its prepn Download PDFInfo
- Publication number
- CN1453572A CN1453572A CN 02118705 CN02118705A CN1453572A CN 1453572 A CN1453572 A CN 1453572A CN 02118705 CN02118705 CN 02118705 CN 02118705 A CN02118705 A CN 02118705A CN 1453572 A CN1453572 A CN 1453572A
- Authority
- CN
- China
- Prior art keywords
- monomer
- content
- medical
- relation
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The present invention is detection method of medical polyacrylamide hydrogel in its preparation. Ultraviolet spectrophotometer is used in measuring the content of acrylamide monomer in the washing water of polymerization product. The specific process includes the following steps: compounding reference monomer solution in advance, measuring the ultraviolet absorbency A of reference solution in different concentration C, drawing C-A curve in planar coordinate, determining the range of qualified product, measuring the ultraviolet absorbency A1 of washing water in the preparing medical polyacrylamide hydrogel, comparing A1 with the C-A curve to find the monomer content. Compared with available liquid chromatographic process, the method has 60-100 times faster speed, and the method may be used directly in monitoring the production process.
Description
Technical field
The present invention relates to the detection method of unconverted monomer in a kind of detection method of high molecular polymer, particularly medical hydrogel of polyacrylamide.
Background technology
Polyacrylamide hydrophilic gel has been widely used in medical field, as electrophoresis material, and coated material, the interior defect packing material of human body etc.Polyacrylamide hydrophilic gel especially for filling human body, requires wherein unpolymerized acrylamide monomer content to be controlled in few scope as medical material.Current now standard is 10
-6Below (weight ratio of monomer and contained monomer gel).Because acrylamide monomer is more much higher than the standard of permitting using with crosslinking chemical content of monomer in the polymkeric substance after polyreaction under the action of evocating, therefore, for making polyacrylamide hydrophilic gel meet medical requirement, processing procedure again after the polyreaction also to be arranged, as the part of the complete preparation process of medical hydrogel of polyacrylamide.Because polyacrylamide hydrophilic gel is subjected to influence of various factors in polymerization process, the content of monomer is difficult to control.So will carry out the strictness check to the finished product hydrogel.The method of inspection in the past all is to take a sample from the finished product hydrogel, soaks the extraction monomer, and the monomer distribution balance is used content of monomer in the liquid chromatography for measuring extract after evenly again in water and gel, is used for determining the gel content of monomer.This detection method, because detection is final product, in a single day so defective, product is waste product.In addition, this method requires sample weighing precision height, and process is complicated, and the time is long, and error is big.
Summary of the invention
The objective of the invention is to provide a kind of simple and direct, detection method of content of monomer in the polyacrylamide hydrophilic gel fast and accurately, and this method is directly used in the preparation process of hydrogel, to guarantee to prepare specification product.
Technical scheme of the present invention is to adopt the determined by ultraviolet spectrophotometry polyreaction to finish the back to remove cleansing solution in the impurity process such as monomer, and content of monomer is reduced to conformance with standard (10 in cleansing solution
-6Below) time, stop handling again, get the medical aquogel product.The step of said ultraviolet spectrophotometry is: measure and draw out the concentration C of acrylamide monomer solution of the selected light wave length and the adjacent concentration range of content that conforms to quality requirements and the relation curve (C-A relation line) of absorbance A earlier.An one-variable linear regression equation y=k+bx (k, b are constant) when this relation line can also directly convert.
During actual measurement, get after the polyreaction again the washings in the processing procedure, measure its absorbance A 1 with spectrophotometric, contrast above-mentioned relation figure, or numerical value substitution equation promptly drawn content of monomer.As exceed standard, then continue processing procedure again, as below regulation numerical value, then stop handling again, get finished product.
Use good effect of the present invention to be: at first this method time short, generally only need 3-5 minute, and liquid phase chromatography was before wanted 3-5 individual hour.Both required times differ 60-100 doubly.In addition,, make it and directly among actual production process, to use, carry out with machine testing: the generation of having stopped substandard product because this method is quick, easy.
Accompanying drawing
Accompanying drawing is the concentration C of acrylamide monomer solution used in the present invention and the graph of a relation of ultraviolet absorbance A (C-A graph of a relation).
Embodiment
1, the front that concerns of acrylamide monomer solution concentration C and ultraviolet absorbance A is drawn.
With distilled water acrylamide monomer is mixed with 0.1mg/ml, 0.01mg/ml, 0.001mg/ml, 0.0001mg/ml, the solution of four kinds of normal concentrations.But select the ultraviolet light source of emission wavelength 210nm.Scan the solution of above-mentioned four kinds of concentration with the ultraviolet ray of this wavelength, on plane coordinates, mark four measured absorbance A
1-4, the each point line is got the C-A relation line of accompanying drawing, equation is y=0.00067+0.001574x during conversion.
2, actual measurement
Be monomer with the acrylamide, N, the two propionamides of N dimethyl are crosslinking chemical; On doctor's hydrogel production line that initiating agent that tetramethyl second diamino, ammonium persulfate, sodium bisulfite are formed and redistilled water are raw material, get polyreaction after, remove and intend the washings that dump in the processing procedure again of monomer.Measure absorbance A 1 with ultraviolet spectrophotometer, should value and the C-A graph of a relation make reference.Or the substitution equation can draw the hydrogel content of monomer of this moment and be what, and whether with conformance with standard, and whether decision continue processing procedure again, and assurance finally obtains specification product.
The employed ultraviolet wavelength of present embodiment can be selected in the 190-220nm scope.The normal concentration solution of drawing the C-A relation line can be more than four kinds, and the C-A line of being drawn can be more accurate.
Claims (1)
- Detection method in a kind of medical hydrogel of polyacrylamide preparation process is characterized in that adopting the determined by ultraviolet spectrophotometry polyreaction to finish the back and removes cleansing solution in the impurity process such as monomer, and content of monomer is reduced to conformance with standard (10 in cleansing solution -6Below) time, stop handling again, get the medical aquogel product, the step of said ultraviolet spectrophotometry is: measure and draw out the concentration C of acrylamide monomer solution of the selected light wave length and the adjacent concentration range of content that conforms to quality requirements and the relation curve (C-A relation line) of absorbance A earlier, an one-variable linear regression equation y=k+bx (k, b are constant) when this relation line can also directly convertDuring actual measurement, get after the polyreaction again the washings in the processing procedure, measure its absorbance A 1 with spectrophotometric, contrast above-mentioned relation figure, or numerical value substitution equation promptly drawn content of monomer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02118705 CN1453572A (en) | 2002-04-23 | 2002-04-23 | Detection of medical polyacrylamide hydrogel during its prepn |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02118705 CN1453572A (en) | 2002-04-23 | 2002-04-23 | Detection of medical polyacrylamide hydrogel during its prepn |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1453572A true CN1453572A (en) | 2003-11-05 |
Family
ID=29257416
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02118705 Pending CN1453572A (en) | 2002-04-23 | 2002-04-23 | Detection of medical polyacrylamide hydrogel during its prepn |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1453572A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100543456C (en) * | 2007-07-19 | 2009-09-23 | 广州天赐高新材料股份有限公司 | A kind of lithium bis (oxalato) borate content ultraviolet spectrophotometry |
| CN102419306A (en) * | 2011-08-19 | 2012-04-18 | 东华大学 | Method for measuring ultraviolet ray filtering of different liquids through sunshine ultraviolet spectrum |
| CN102590121A (en) * | 2011-05-05 | 2012-07-18 | 重庆大学 | Method for measuring content of residue acrylamide monomer in water treatment agent cationic polyacrylamide |
| CN105588809A (en) * | 2015-12-15 | 2016-05-18 | 新疆科力新技术发展股份有限公司 | Method for measuring concentration of polyacrylamide in oil field polymer-flooding produced liquid |
| CN106053644A (en) * | 2016-06-02 | 2016-10-26 | 西安石油大学 | Method for detecting amazingel used in medical cosmetic and plastic surgery material |
| CN109580516A (en) * | 2019-01-14 | 2019-04-05 | 广州城市职业学院 | A kind of detection method of polyacrylamide |
-
2002
- 2002-04-23 CN CN 02118705 patent/CN1453572A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100543456C (en) * | 2007-07-19 | 2009-09-23 | 广州天赐高新材料股份有限公司 | A kind of lithium bis (oxalato) borate content ultraviolet spectrophotometry |
| CN102590121A (en) * | 2011-05-05 | 2012-07-18 | 重庆大学 | Method for measuring content of residue acrylamide monomer in water treatment agent cationic polyacrylamide |
| CN102590121B (en) * | 2011-05-05 | 2014-01-15 | 重庆大学 | Method for measuring content of residue acrylamide monomer in water treatment agent cationic polyacrylamide |
| CN102419306A (en) * | 2011-08-19 | 2012-04-18 | 东华大学 | Method for measuring ultraviolet ray filtering of different liquids through sunshine ultraviolet spectrum |
| CN105588809A (en) * | 2015-12-15 | 2016-05-18 | 新疆科力新技术发展股份有限公司 | Method for measuring concentration of polyacrylamide in oil field polymer-flooding produced liquid |
| CN106053644A (en) * | 2016-06-02 | 2016-10-26 | 西安石油大学 | Method for detecting amazingel used in medical cosmetic and plastic surgery material |
| CN106053644B (en) * | 2016-06-02 | 2018-06-29 | 西安石油大学 | A kind of medical and beauty treatment shaping material Elipten AG detection method |
| CN109580516A (en) * | 2019-01-14 | 2019-04-05 | 广州城市职业学院 | A kind of detection method of polyacrylamide |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106770851B (en) | A kind of mobile phase formula of liquid chromatography for measuring carotenoid and its application | |
| CN1453572A (en) | Detection of medical polyacrylamide hydrogel during its prepn | |
| CN112730720B (en) | Liquid phase detection method for vitronectin in cosmetics | |
| CN107907603A (en) | A kind of measure assay method of the amino acids parenteral solution tryptophan in relation to material | |
| CN111443149A (en) | Method for measuring content of lysine hydrochloride in sodium hyaluronate composite solution for injection | |
| CN1841047A (en) | Elution test method and apparatus | |
| CN113341028A (en) | Method for measuring sodium hyaluronate molecular weight and molecular weight distribution by size exclusion chromatography | |
| CN108680680A (en) | Method for measuring peak-out time of reactive monomer in chromatogram and concentration of reactive monomer in liquid crystal | |
| CN108801952A (en) | A kind of detection method of the free hyaluronic acid sodium content of medical cross-linking sodium hyaluronate gel | |
| CN112147095A (en) | Method for rapidly determining crosslinked sodium hyaluronate gel protein residue | |
| CN104877147B (en) | The preparation method and application of PVA HEA ultraviolet 3-dimensional dose meters | |
| CN106645751B (en) | A kind of fibrinogen content detection kit | |
| CN107941966A (en) | A kind of method based on headspace gas chromatography measurement guar gum molecular weight | |
| CN104614335A (en) | Method for rapidly identifying theabrownin | |
| CN107957403B (en) | Method for measuring chitosan content by using carmine as probe through ultraviolet spectrophotometry | |
| CN111220676B (en) | Method for detecting purity of protein sample containing polyethylene glycol by using capillary electrophoresis technology | |
| CN108827945A (en) | A kind of method of biuret method measurement Rofe source of fish collagen peptide content | |
| CN113667144A (en) | Composite hydrogel array for visually detecting metal ions and preparation method and application thereof | |
| CN117554535B (en) | Detection method and kit for detecting oxalic acid in human urine by liquid chromatography | |
| CN110568040B (en) | Method for testing content of arbutin in cosmetics by electrochemically polymerizing DL-aspartic acid modified electrode | |
| CN107036989B (en) | A method of measurement chrome tanning agent active constituent content | |
| CN114994211B (en) | Kit for detecting catecholamine metabolite content in human urine and application thereof | |
| KR102146957B1 (en) | Device and method that tests drinking water using Colorimeter | |
| CN113391025A (en) | Method for measuring moisture of implant | |
| CN105158222B (en) | A kind of measuring method of fluorescent quantitation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |