CN1437021A - Biological information analyzer and analysis method - Google Patents
Biological information analyzer and analysis method Download PDFInfo
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- CN1437021A CN1437021A CN 02113962 CN02113962A CN1437021A CN 1437021 A CN1437021 A CN 1437021A CN 02113962 CN02113962 CN 02113962 CN 02113962 A CN02113962 A CN 02113962A CN 1437021 A CN1437021 A CN 1437021A
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Abstract
The biological information analyzer includes detecting pond, quartz vibrator circuit, frequency counter circuit, microprocessor, RS232 circuit, temperature controller and display circuit. Owing to the high sensitivity response of quartz crystal, the tiny characteristic change of solution in the detecting pond may be reflected to the vibration frequency of the quartz vibrator circuit and the change is detected by the precise frequency counter, processed in the microprocessor and analyzed via the specialist data base in PC to output the result. The biological information analyzer has simple structure, low cost, wide application range, fast detection speed and high sensitivity.
Description
Technical field:
The present invention relates to a kind of medical check and analysis instrument field, be meant a kind of biological information analyzer and analytical approach of utilizing the reaction of some specificity of piezoelectric quartz crystal sensor detection of biological especially.
Technical background:
In medical diagnosis and treatment process, general all to utilize medical check and analysis instrument that patient's body fluid such as blood, urine, saliva etc. are detected, can accelerate diagnostic procedure like this, also can improve the accuracy of diagnosis simultaneously, improve the quality of diagnosis and treatment, reduce the generation of mistaken diagnosis phenomenon.Present medical check and analysis instrument mainly contains two big classes: a class is the manual analysis instrument, as microscope etc., microscopical check and analysis method is that thing to be detected is applied on the glass sheet, be placed on microscopically again and observe, the shortcoming that this method exists is that labour intensity is big, inefficiency.Human eye places microscopically to observe for a long time, and eyes are tired easily, the easy like this observations mistake that causes, and observations is subjected to operator's factor affecting such as knowledge, experience and psychological condition simultaneously, can not realize standardization, and can not carry out quantitative test.Another kind of is automatic analyzer, and this quasi-instrument detection speed is very fast, and can carry out quantitative test, and its testing result is not subjected to operator's influence substantially, can realize standardization.But this quasi-instrument complex structure, cost height, and sensing range is narrow, is not easy to promote the use of.
Summary of the invention:
The objective of the invention is to overcome the deficiency of prior art and provide a kind of simple in structure, cost is low, detect and the biological information analyzer wide, that efficient is high.
Another object of the present invention provides a kind of biological information detection method.
For achieving the above object, the present invention includes
One detection cell is used to contain liquid to be detected;
One crystal oscillation circuit, be used for producing one with the relevant oscillator signal of characteristic of detection cell liquid to be detected;
One frequency counting circuit is used under the control of microprocessor, and the oscillator signal of crystal oscillation circuit output is counted;
One microprocessor is used for the controlled frequency counting circuit signal of crystal oscillation circuit output is counted, and control display circuit demonstration count results, simultaneously count results is handled, and the data after will handling is delivered to PC by the RS232 serial port circuit.
One RS232 circuit is used for communicating by letter between microprocessor and the PC.
Above-mentioned device also comprises a display circuit, is used to show the crystal oscillation circuit output signal frequency.
Above-mentioned device also comprises a temperature regulating device, is used for the temperature in control detection pond, and described temperature regulating device is made up of calibration cell, bulb, automatic temp controller.
Above-mentioned crystal oscillating circuit is made up of Sheffer stroke gate U7A, U7B, capacitor C 4 ~ C6, resistance R 12 ~ R13 and crystal Y1, after Sheffer stroke gate U7A and capacitor C 4, resistance R 13 are in parallel, one termination capacitor C5, another termination crystal Y1, the other end of crystal Y1 with meet detection cell electrode L1 after capacitor C 6 is connected, one end of the input end of the other end of C5 and Sheffer stroke gate U7B, resistance R 12 joins, and the other end of resistance R 12 and the output terminal of U7B, detection cell electrode L2 join.
A kind of analysis of biological information method is characterized in that: may further comprise the steps:
1) biofluid to be detected is placed detection cell;
2) utilize quartz oscillator to produce an oscillator signal relevant with the characteristic of biofluid to be detected;
3) oscillator signal is carried out frequency counting;
4) count results is differentiated, and operation result and expert database are compared, the output analysis result.
The present invention utilizes highly sensitive quality of quartz crystal and non-mass-basis response characteristic, quartz crystal is put into solution or make quartz crystal single face contact liq, thereby the resonance frequency of crystal oscillation circuit is changed with the variation of density, viscosity and the conductivity of solution, obtain testing result by the frequency change that detects crystal oscillation circuit, it has the following advantages:
1, simple in structure, cost is low.
2, detection is wide.Can carry out parasite, bacterium, virus, red blood cell, leucocyte, blood platelet, amino acid, protein, enzyme, fat, carbohydrate, vitamin and mensuration micro-and that contain the medicine of organic bromine and organic iodine at biological, chemistry, medical domain.
3, detection speed is fast, sensitive high.Utilize highly sensitive quality of quartz crystal and non-mass-basis response characteristic, the subtle change of solution properties can be reflected to the variation of the oscillation frequency of crystal oscillation circuit in the detection cell, detects by accurate frequency counter again.Simultaneously, whole testing process need not artificial participation substantially, has improved work efficiency greatly.The invention will be further described below in conjunction with drawings and the specific embodiments.
Description of drawings:
Fig. 1 is an electric theory diagram of the present invention.
Fig. 2 is an electrical schematic diagram of the present invention.
Embodiment:
Referring to Fig. 1, the utility model comprises a crystal oscillation circuit 2, be used for producing one with the relevant oscillator signal of characteristic of detection cell liquid to be detected; One temperature regulating device 4, be made up of calibration cell, bulb, automatic temp controller, detection cell 1 places on the calibration cell, and the temperature of may command detection cell is between room temperature to 100 ℃, the optimal reaction temperature of considering biosome is 37 ℃, and the temperature in general control detection pond is about 37 ℃; One frequency counting circuit 3 is used under the control of microprocessor 6, and the signal of crystal oscillation circuit 2 outputs is counted; One display circuit 7 is used to show the crystal oscillation circuit output signal frequency; One microprocessor 6, the signal that is used for 2 outputs of 3 pairs of crystal oscillation circuits of controlled frequency counting circuit is counted, and control display circuit demonstration count results, simultaneously count results is handled, and the data after will handling are delivered to PC by RS232 serial port circuit 5; One RS232 circuit 5 is used for communicating by letter between microprocessor 6 and the PC.
Referring to Fig. 2, it is the circuit diagram of concrete enforcement of the present invention, and switch SW 2, transformer T1, rectifier bridge D0, integrated voltage stabilizer U0, U01, U02 and peripheral circuit thereof are formed power circuit among the figure, and the whole system operation power supply is provided.Sheffer stroke gate U7A, U7B, capacitor C 4 ~ C6, resistance R 12 ~ R13 and crystal Y1 form crystal oscillating circuit, be connected in series an electrolytic cell in the series loop of crystal simultaneously, the impedance of the solution of electrolytic cell, electricity are led like this, admittance, resistance, the isoparametric variation of capacitive reactance can make oscillation frequency change, and capacitor C 6 is used for finely tuning oscillation frequency; Integrated circuit U7 and peripheral circuit component frequency counting circuit thereof, U7 is frequency counting circuit ICM7216, crystal Y2, resistance R 2, capacitor C 2 ~ C3 form the clock oscillation circuit of counting circuit, and the reference clock signal of counting is provided, and the counting of U7 is subjected to the control of microprocessor U1; Charactron DS1 ~ DS7 and peripheral circuit thereof are formed display circuit, under the control of microprocessor U1, are used for showing the count results of U7.Microprocessor U1 and peripheral circuit thereof are formed little control and treatment circuit, the model of U1 is 89C52, crystal Y3, capacitor C 1 ~ C2 form the clock oscillation circuit of U1, microprocessor U1 accepts the PC instruction, controlled frequency counting circuit U7 counts crystal oscillation circuit, and control display circuit demonstration count results, the result to counting circuit handles simultaneously, and delivers to PC by serial ports and handle.
Concrete check and analysis method of the present invention is as follows: when detecting, biological reagent to be detected is placed detection cell, the reaction of some specificity of biology to be measured in the detection cell: as antigen-antibody reaction, specific enzyme reaction, the impedance of mass change that absorption of proteins causes and the nutrient solution that in the microbe growth process, causes, electricity is led, admittance, changes in resistance, the impedance of nutrient solution, electricity is led, admittance, changes in resistance can make the crystal oscillation circuit oscillation frequency change, microprocessor U1 accepts the PC instruction, controlled frequency counting circuit U7 counts the output signal of crystal oscillation circuit, and the control display circuit shows count results, result to counting circuit handles simultaneously, and deliver in the microcomputer by the RS232 interface circuit, microcomputer is gathered the data that microprocessor is sent here, and handle, expert database in the last and microcomputer compares, automatically differentiate, and print net result.
Claims (5)
1, a kind of biological information analyzer is characterized in that: comprise
One detection cell is used to contain biofluid to be detected;
One crystal oscillation circuit, be used for producing one with the relevant oscillator signal of characteristic of detection cell liquid to be detected;
One frequency counting circuit is used under the control of microprocessor, and the oscillator signal of crystal oscillation circuit output is counted;
One microprocessor is used for the controlled frequency counting circuit signal of crystal oscillation circuit output is counted, and control display circuit demonstration count results, simultaneously count results is handled, and the data after will handling is delivered to PC by the RS232 serial port circuit;
One RS232 circuit is used for communicating by letter between microprocessor and the PC.
2, biological information analyzer according to claim 1 is characterized in that: also comprise a display circuit, be used for the crystal oscillation circuit output signal frequency.
3, biological information analyzer according to claim 1 and 2 is characterized in that: also comprise a temperature regulating device, be used for the temperature in control detection pond, described temperature regulating device is made up of calibration cell, bulb, automatic temp controller.
4, biological information analyzer according to claim 1, it is characterized in that: described crystal oscillating circuit is made up of Sheffer stroke gate U7A, U7B, capacitor C 4 ~ C6, resistance R 12 ~ R13 and crystal Y1, after Sheffer stroke gate U7A and capacitor C 4, resistance R 13 are in parallel, one termination capacitor C5, another termination crystal Y1, the other end of crystal Y1 with meet detection cell electrode L1 after capacitor C 6 is connected, one end of the input end of the other end of C5 and Sheffer stroke gate U7B, resistance R 12 joins, and the other end of resistance R 12 and the output terminal of U7B, detection cell electrode L2 join.
5, a kind of analysis of biological information method is characterized in that: may further comprise the steps:
1) biofluid to be detected is placed detection cell;
2) utilize quartz oscillator to produce an oscillator signal relevant with the characteristic of biofluid to be detected;
3) oscillator signal is carried out frequency counting;
4) the frequency counting result is differentiated, and operation result and expert database are compared, the output analysis result.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02113962 CN1280628C (en) | 2002-02-06 | 2002-02-06 | Biological information analyzer and analysis method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02113962 CN1280628C (en) | 2002-02-06 | 2002-02-06 | Biological information analyzer and analysis method |
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| Publication Number | Publication Date |
|---|---|
| CN1437021A true CN1437021A (en) | 2003-08-20 |
| CN1280628C CN1280628C (en) | 2006-10-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 02113962 Expired - Fee Related CN1280628C (en) | 2002-02-06 | 2002-02-06 | Biological information analyzer and analysis method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100338458C (en) * | 2004-05-24 | 2007-09-19 | 湖南大学 | Method and apparatus for detecting microbe by piezoelectric quartz crystal sensor |
| CN101784895A (en) * | 2007-06-26 | 2010-07-21 | 协乐民公司 | Method for predicting biological systems responses in hepatocytes |
| CN108828335A (en) * | 2017-04-21 | 2018-11-16 | 艾力集团有限责任公司-卡皮贾尼 | The device and method for determining bacterium charge (charge) in liquid or semi-fluid product |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101231286B (en) * | 2008-02-21 | 2011-09-07 | 江苏省人民医院 | Application of acetylcholine esterase piezoelectricity biosensor in the detection of nerve tract property |
-
2002
- 2002-02-06 CN CN 02113962 patent/CN1280628C/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100338458C (en) * | 2004-05-24 | 2007-09-19 | 湖南大学 | Method and apparatus for detecting microbe by piezoelectric quartz crystal sensor |
| CN101784895A (en) * | 2007-06-26 | 2010-07-21 | 协乐民公司 | Method for predicting biological systems responses in hepatocytes |
| CN108828335A (en) * | 2017-04-21 | 2018-11-16 | 艾力集团有限责任公司-卡皮贾尼 | The device and method for determining bacterium charge (charge) in liquid or semi-fluid product |
| CN108828335B (en) * | 2017-04-21 | 2021-12-21 | 艾力集团有限责任公司-卡皮贾尼 | Device and method for determining the charge of bacteria in a fluid or semifluid product |
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| Publication number | Publication date |
|---|---|
| CN1280628C (en) | 2006-10-18 |
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