CN1435248A - Chinese medicine preparation for treating upper respiratory system diseases, skin infection and pile, and preparing method thereof - Google Patents
Chinese medicine preparation for treating upper respiratory system diseases, skin infection and pile, and preparing method thereof Download PDFInfo
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Abstract
A Chinese medicine in the form of tablet, capsule, pill, particle, or oral liquid for treating upper respiratory tract infection, local skin infection and pile is prepared from scrophularia root and sarcandra glabra through crushing, decocting, concentrating, depositing in alcohol, recovering alcohol, concentrating, and shaping.
Description
Technical field
The present invention relates to a kind of Chinese medicine preparation, treat particularly that upper respiratory tract infection, local skin infect and the Chinese medicine preparation of hemorrhoid preparation method extremely.
Background technology
Acute and chronic pharyngitis, tonsillitis, gingivitis, furuncle are common diseases, and the main diseases of these diseases is because of being to suffer from cold, tired, tobacco and wine are excessive, various physics and chemical stimulation, malnutrition, the low grade of passive protective physical fitness causes, the symptom of its upper respiratory tract infection has pharyngeal drying respectively, and is scorching hot, pain or odynophagia, gum edge, gum nipple congestion and edema, throat has foreign body to stop up sense, and hoarseness brings up phlegm when one coughs, the symptom of furuncle is the local skin red and swollen heat pain, heating when serious, fear of cold, general malaise, inappetence, headache, nape and extremities aching pain.The normal medicine that uses to upper respiratory tract and local skin infection western medical treatment is an antibiotics at present, as penicillin, erythromycin, cephalosporin, midecamycin, tetracycline etc.; Though Western medicine and antibiotics are better to treatment for diseases effect caused by the bacteria, if commonly used and abuse antibiotics not only can make some antibacterials produce drug-resistant effects, the intravital beneficial bacteria of people can not be survived, and cause that other disease occurs or increases the weight of.The Chinese medicine of many treatment upper respiratory tract and local skin infection is summed up and excavated to folks of china for thousands of years, Chinese herbal medicine commonly used has Herba Andrographis, Rhizoma Coptidis, Cortex Phellodendri, Fructus Forsythiae, Flos Lonicerae, Herba Houttuyniae, Herba Violae, Flos Chrysanthemi, Spica Prunellae, the Radix Pulsatillae, Herba Lobeliae Chinensis etc., these Chinese medicines have played certain effect to treatment upper respiratory tract and local skin infection respectively, but single Chinese medicine has limitation, have to just working than hypopathia disease, effect was relatively poor when disease was heavy.In secular medical practice, people get up some Chinese medicine composition, be made into Chinese medicine preparation, Chinese medicine preparation commonly used has Herba Pileae Scriptae Tabellae, JIANMIN YINHOU PIAN, the benzydamine sheet, YINHUANG PIAN, Radix Isatidis granules, Qinghuozhimaipian etc., these Western medicine, Chinese medicine and Chinese medicine preparation are coughed up phlegm to patient's cough, the nasal obstruction watery nasal discharge, rhinocnesmus sneeze, have sore throat, hoarseness, heating, headache, dry pharynx is scorching hot, pharyngeal hyperemia, symptom and signs such as vocal congestion and pharyngeal tonsils hyperemia all have clear improvement, people still continue many Chinese medicines are studied at present, to excavate more Chinese medicine, make the Chinese medicine preparation of treatment various diseases, be mankind's service.
Technology contents
The applicant is just on the basis of research tradition Chinese herbal medicine, through test for many years, the prescription in conjunction with Guangxi treatment upper respiratory tract more among the people and local skin infection screens multiple formulations, found therapeutic effect good, the several drugs that has no side effect, worked out a kind of new Chinese medicine preparation, said preparation comprises Radix Picriae felterrae and Herba Sarcandrae, and it has heat-clearing and toxic substances removing, the effect of reducing swelling and alleviating pain, can be used for inflammation of upper respiratory tract, bronchitis, rhinitis, pharyngolaryngitis, symptoms such as tonsillitis, also can treat diseases such as gingivitis, furuncle, through clinical trial in a few years, proved that this medicine produce effects is fast, subjective symptoms and sign are obviously improved, and not seeing has side effect, and are difficult for recurrence.
Chinese medicine preparation of the present invention comprises Radix Picriae felterrae and Herba Sarcandrae, and the composition of its primary raw material and weight content are:
Radix Picriae felterrae 5～95%
Herba Sarcandrae 95～5%
This Chinese medicine preparation comprises tablet, capsule, pill, granule, and its preparation method is as follows:
1, the production method of tablet: Radix Picriae felterrae and Herba Sarcandrae washed respectively cut, fragmentation, add 6～10 times of water heated and boiled 1.5～3 hours by the per kilogram medicine, take out filtrate, filter, medicinal residues added water boil 1～2 hour again, and merging filtrate concentrates, add ethanol precipitation, supernatant reclaims ethanol, concentrates, and makes granule with an amount of filler, disintegrating agent, tabletting, coating obtains product.
The described filler of above tablet is starch, dextrin, carboxymethyl starch, calcium hydrogen phosphate and other pharmaceutic adjuvants; Described disintegrating agent is sodium carboxymethyl cellulose, hydroxypropyl cellulose and pharmaceutic adjuvant with water swelling; Described lubricant is the pharmaceutic adjuvant of Polyethylene Glycol, magnesium stearate, Pulvis Talci and tool lubrication.Wherein the weight content of filler, disintegrating agent is respectively: 45～75%, 0.5～10%, and the lubricant weight content is: 0.5～10%
2, the production method of capsule: add filler, disintegrating agent and fluidizer in the concentrated solution that above-mentioned production is obtained, and make it dry, or make granule or make during micropill incapsulates then, obtain product.
More than the described filler of preparation capsule is starch, dextrin, carboxymethyl starch, calcium hydrogen phosphate and other pharmaceutic adjuvants; Described disintegrating agent is sodium carboxymethyl cellulose, hydroxypropyl cellulose and pharmaceutic adjuvant with water swelling; Described fluidizer is that magnesium stearate, differential silica gel and other have the pharmaceutic adjuvant of fluidizer effect.Filler, disintegrating agent, fluidizer weight content are: 30～70%, 0.5～20%, 0.5～5%.
3, the production method of pill: add filler in the concentrated solution that above-mentioned production is obtained, stir, make pill, and make it dry, or coating promptly obtains product.
The composition and the content that add filler: medicinal residues xeraphium after the extraction and starch (or other pharmaceutic adjuvants) 30～75%, thin film coating material 2～5%.
4, the production method of granule: in the concentrated solution that above-mentioned production obtains, add filler, make granule, make it dry, obtain product.
The filler that adds is sucrose, lactose, dextrin, starch.
5, the preparation method of oral liquid is: Radix Picriae felterrae and Herba Sarcandrae are washed respectively cut, fragmentation, add 6～10 times of water heated and boiled 1.5～3 hours by the per kilogram medicine, take out filtrate, filter, medicinal residues added water boil 1～2 hour again, merging filtrate concentrates, and adds ethanol precipitation, supernatant reclaims ethanol, concentrate, in the concentrated solution that above-mentioned production obtains, add sucrose, Mel or sweeting agent, antiseptic, obtain product.
Outstanding substantive distinguishing features of the present invention and obvious improvement are:
Guangxi traditional Chinese herbal medicine Radix Picriae felterrae and Herba Sarcandrae have been adopted, the product heat-clearing and toxic substances removing of presenting a memorial to the emperor altogether, the effect of reducing swelling and alleviating pain.To acute and chronic pharyngitis, tonsillitis, gingivitis, furuncle, hemorrhoid have significant curative effect.Following explanation earlier side of the present invention separate:
Radix Picriae felterrae: the dry herb for goatweed Radix Picriae felterrae Picria fel-terrae Lour has another name called Herba vallisneriae Spiralis.Mainly be distributed in ground such as Guangxi, Guangdong and Yunnan, among the people in Guangxi as medicinal existing very long history.The Radix Picriae felterrae flavor is extremely bitter, and cold in nature, its main component is a flavonoid, picfeltarraenin A, B, and have heat-clearing and toxic substances removing, removing heat from blood repercussive effect: the main effective ingredient of Radix Picriae felterrae is the tetracyclic triterpene glycoside, wherein based on Radix Picriae felterrae glycosides IA and IB.The heat-clearing and toxic substances removing of Radix Picriae felterrae glycosides, reducing swelling and alleviating pain, anti-inflammation and sterilization effect are particularly outstanding.Be usually used in cold anemopyretic, laryngopharynx swelling and pain, mumps, dysentery etc.Modern pharmacology discovers, the decocting liquid of Radix Picriae felterrae is to staphylococcus aureus, bacillus pyocyaneus, and escherichia coli, wax-shaped bacillus, Bacillus typhi, bacillus subtilis, dysentery bacterium etc. all have tangible bacteriostasis.Compare with general antibacterial plant medicine (as Herba Andrographis etc.), it is strong 5 times to tire, and antimicrobial spectrum is also wider.
Herba Sarcandrae: the herb for Chloranthaceae plant plait Corallium Japonicum Kishinouye (Sarcandra glabra Thunb) has another name called Herba Pileae Scriptae, Ramulus Sambuci Williamsii etc.Have effects such as antiinflammatory, heat clearing away, detoxifcation and relaxing muscles and tendons to promote blood circulation.Main component is Fumaric acid, isofraxidin, volatilization wet goods.Be usually used in treating inflammation of upper respiratory tract clinically, bronchitis, rhinitis, pharyngolaryngitis, tonsillitis, bacillary dysentery and chronic cholecystitis.Prove that through clinical practice rapid-action to above-mentioned disease, the cure rate height is an one of medicine first choice of the above-mentioned respiratory system disease of treatment.It is reported that the main component of Herba Sarcandrae is flavonoid glycoside, nitrile glycoside, coumarone, lactone, tannic acid, volatile oil.Domestic have bibliographical information to separate from Herba Sarcandrae and identified linalyl acetate and Isofraxidin Loifniddh.The Tokyo pharmaceutical university of Japan refers to contain Loifniddh.9-paddy coloured glaze alcohol radical 9-D-glycoside, Astilbin, sugar etc. in the report ZHONGJIEFENG ZHUSHEYE (folk prescription) such as field.The clinical application range of Herba Sarcandrae is extensive, and particularly the diseases associated with inflammation to respiratory system, digestive system has higher curative effect.According to many periodicals and patent report, Herba Sarcandrae is treated orthopaedics and antitumaous effect in addition, as Chinese patent＜application number〉95100286＜denomination of invention〉a kind of Chinese medicine anti-cancer capsule prescription and its formula components of preparation method be: Pelargonium gravelens 5-10 part, open up wooden 5-10 part, Herba Agrimoniae 5-10 part, Herba Hedyotidis Diffusae 5-10 part, Radix Patriniae 5-10 part, Herba Taxilli 5-10 part, Herba Sarcandrae 5-10 part, Folium Mayteni hookeri 5-10 part, Rabdosia rubescens 5-10 part, Radix Ginseng 6-10 part, Ganoderma 6-10 part.Through tens of routine clinical treatment observations, cancerous cell there is good inhibitory effect, take avirulence for a long time, side effect is little, and is harmless to the hepatic and renal tissue of human body, taking convenience, the part patients clinical is cured.
2, the pharmacological action of this Chinese medicine preparation:
A, the relevant antibacterial of tracheitis screening had in quick inhibitory action (first streptococcus, micrococcus catarrhalis, hemophilus influenza, Diplococcus pneumoniae) to Gao Min, to golden Portugal bacterium, dysentery bacterium, Bacillus typhi and bacillus pyocyaneus have stronger bacteriostasis.This is the basis of WANTONG YANKANG PIAN tool antibacterial and anti-inflammation functions.
B, antivirus action: influenza virus is had deactivation.
C, antitumor action: the spontaneous breast carcinoma Ca615 of experiment mice, mice S180 there are obvious tumor-inhibiting action, but to spontaneous leukemia ascitic type life-saving.
D, immunization: improve cytophagy, act on similar to Radix Ginseng.
E, antiulcer: the zoopery proof is similar to aluminum sulfate to the effect of erosive gastritis, and gastric mucosa is formed protecting film, promotes the reparation of ulcer.
3, use: except treating acute and chronic pharyngitis, tonsillitis, gingivitis is beyond the furuncle, also can treat diseases such as local skin infection and hemorrhoid, also have antitumaous effect (cancer of pancreas, gastric cancer, rectal cancer, hepatocarcinoma, the esophageal carcinoma), oral inflammation, gastric ulcer, hemorrhoid etc. are also had effect.
4, the clinical symptoms efficacy result shows, adopt own control contrast therapy, after two groups of patient cough cough up phlegm, have a stuffy nose watery nasal discharge, rhinocnesmus sneeze, have sore throat, symptom and signs such as hoarseness, heating, headache, dry pharynx are scorching hot, pharyngeal hyperemia, vocal congestion and throat hyperemia all have clear improvement.But two groups relatively, and difference does not have the significance meaning.The clinical symptoms of pointing out Chinese medicine preparation of the present invention can obviously improve wind-heat syndrome (acute rhinitis, acute pharyngitis, acute laryngitis, acute tonsillitis), similar to the contrast medicine.
5, product of the present invention has carried out blood, urine, just routine examination to 290 many cases patients, and the heart, liver, kidney function test are not all found obvious adverse reaction and toxic and side effects.Prompting this product is clinical safe and reliable.
6, clinical verification, product of the present invention has the effect of heat-clearing and toxic substances removing, reducing swelling and alleviating pain, is used for inflammation of upper respiratory tract, bronchitis, rhinitis, pharyngolaryngitis, tonsillitis.In order further to investigate its curative effect, attending scope and untoward reaction, according to " clinical disease diagnosis is according to curing the improvement standard ", " new Chinese medicine clinical research guideline ", Guangxi Wu Jia provincial hospital has carried out clinical verification year April in March, 1994 to 2000, treatment group as a result and open treatment group cure rate, obvious effective rate, effective percentage are respectively 20%, 40.8%, 30%, and total effective rate is 90.8%.Point out product of the present invention that acute rhinitis, acute pharyngitis, acute laryngitis, the acute flat body inflammation of choosing are had clinical efficacy preferably.
The specific embodiment
Embodiment 1,100 kilograms of Radix Picriae felterrae and Herba Sarcandrae washed respectively cut, broken, add 6～10 times of water heated and boiled 1.5～3 hours by the per kilogram medicine, take out filtrate, filter, medicinal residues added water boil 1～2 hour again, merging filtrate, concentrate, add ethanol precipitation, supernatant reclaims ethanol, concentrate, add 45～75% filleies such as starch or dextrin, carboxymethyl starch, calcium hydrogen phosphate or other solid medicinal adjuvants, 0.5～10% disintegrating agent carboxymethyl base sodium cellulosate, the pharmaceutic adjuvant of hydroxypropyl cellulose or other tool water swellings and lubricant such as magnesium stearate etc. have lubricated, make granule, tabletting, coating are promptly.This name of product is " WANTONG YANKANG PIAN ".
Embodiment 2,50 kilograms of Radix Picriae felterrae and Herba Sarcandrae washed respectively cut, broken, add 6～10 times of water heated and boiled 1.5～3 hours by the per kilogram medicine, take out filtrate, filter, medicinal residues added water boil 1～2 hour again, merging filtrate, concentrate, add ethanol precipitation, supernatant reclaims ethanol, concentrate, add 40～60% starch, dextrin, carboxymethyl starch, filleies such as calcium hydrogen phosphate, 0.5～10% disintegrating agent carboxymethyl base sodium cellulosate, hydroxypropyl cellulose is made granule and is made it dry, and adds the fluidizer magnesium stearate, differential silica gel, and make it dry, in incapsulating then, obtain product.This name of product is " ten thousand logical scorching recovering capsules ".
Embodiment 3,50 kilograms of Radix Picriae felterrae and Herba Sarcandrae washed respectively cut, fragmentation, add 6～10 times of water heated and boiled 1.5～3 hours by the per kilogram medicine, take out filtrate, filter, medicinal residues added water boil 1～2 hour again, merging filtrate, concentrate, add ethanol precipitation, supernatant reclaims ethanol, concentrate, add medicinal residues xeraphium and starch 30～75% after extracting, thin film coating material 2～5%, stir, make pill, and make it dry, coating promptly obtains product.This name of product is " ten thousand logical scorching health balls ".
Embodiment 4,100 kilograms of Radix Picriae felterrae and Herba Sarcandrae washed cut, fragmentation, add 6～10 times of water heated and boiled 1.5～3 hours by the per kilogram medicine, take out filtrate, filter, medicinal residues added water boil 1～2 hour again, merging filtrate, concentrate, add ethanol precipitation, supernatant reclaims ethanol, concentrates, add filler 20～90% sucrose, lactose, dextrin, starch, make granule, make it dry, obtain product.This name of product is " ten thousand logical scorching recovering particles agent ".
Embodiment 5,100 kilograms of Radix Picriae felterrae and Herba Sarcandrae washed cut, fragmentation, add 6～10 times of water heated and boiled 1.5～3 hours by the per kilogram medicine, take out filtrate, filter, medicinal residues added water boil 1～2 hour again, merging filtrate concentrates, and adds ethanol precipitation, supernatant reclaims ethanol, concentrate, add sucrose or the Mel or the sweeting agent, 0.1～3% of 20～65% weight, obtain the oral liquid product.This name of product is " ten thousand logical scorching recovery oral liquids ".
The relevant toxicity test of the present invention, clinical trial, pharmacology, pharmacodynamics situation:
One, product long term toxicity test report of the present invention (by certain research unit's test)
[test objective]
Observe and repeat continuously to be subjected to the reagent thing, to the toxic reaction that rat produced; Determine nontoxic amounts of reactants, provide reference for drafting the human safe dose.
[being subjected to the reagent thing]
Product of the present invention is the Chinese medicine preparation of Wantong Pharmaceutical Co., Ltd., Guangxi's development.Every in this product tablet contains crude drug 3g, and clinical consumption is 3 of each clothes, and 3 times on the one, 5 days courses of treatment.Being subjected to the reagent thing is the dense thick extractum of this product crude drug, and every g extractum contains crude drug 19.7g, and lot number 991025 is provided by research institute.The medicinal liquid that will be subjected to the reagent thing to be made into desired concn with deionized water during test supplies rat oral gavage (ig) administration, and each dosage group administration volume is equated.
[experiment material]
1. animal: Wistar is a rat, regular grade, and ♀ ♂ dual-purpose, body weight 110～140g, this institute Animal House provides, No. the 11001st, the quality certification number-moving word of osmanthus doctor.Animal ♂ ♀ divide the cage group support, 5 in every cage, and common solid feed is fed.Experiment 20～22 ℃ of room temperatures (air-conditioning), relative humidity 65～80%.
2. instrument: BC-2000 cellanalyzer.
[experimental technique]
Get 80 of rat, ♀ ♂ half and half divides equally 4 groups at random by sex, body weight, and ig is subjected to reagent liquid at 9～10 o'clock in morning every day, and dosage is respectively crude drug 45.0g/kg, 22.5g/kg, 11.25g/kg, and the administration volume is 10ml/kg, 2 weeks of successive administration.Observe the situation such as outward appearance, behavior, extremity activity, feed, Excreta, death of rat during the administration every day.
[result]
1. during the administration and the drug withdrawal viewing duration, each outward appearance, behavior, breathing, extremity activity, feed, drainage etc. of organizing rat shows no obvious abnormalities; There is not the phenomenon of being poisoned to death; Each administration treated animal body weight gain speed is all close with matched group, the difference nonsignificance.
2. hematological examination content of hemoglobin (HGB), RBC number (RBC), leukocyte count (WBC), platelet count (PLT), granulocyte ratio (GRAN), mononuclear cell ratio (MID), lymphocyte ratio (LYMPH), clotting time (CT, capillary tube method) result: after 2 weeks of administration, the WBC of heavy dose of group is a little more than matched group (P＜0.05), heavy dose of group MID is then a little less than matched group (P＜0.05), the PLT of dosage group big or middle is a little more than matched group (P＜0.05), all think to tie up to fluctuation in normal range, do not have a toxicology meaning; All the other project measured values of each administration group and the equal nonsignificance of matched group difference.After 2 weeks of drug withdrawal, the RBC of middle dosage group is a little more than matched group (P＜0.05), and the PLT of large, medium and small dosage group all a little more than matched group (P＜0.01,＜0.05), also thinks to tie up to fluctuation in normal range, do not have a toxicology meaning; Other project measured value of each administration group and the equal nonsignificance of matched group difference.
3. blood biochemical is learned and is checked total serum protein (TP), albumin (ALB), total bilirubin (Bil), blood urea nitrogen (Urea), creatinine (Cr), T-CHOL (TCH), blood glucose (Glu) content, glutamate pyruvate transaminase (ALT), glutamic oxaloacetic transaminase, GOT (AST), the active result of alkali phosphatase (ALP): after 2 weeks of administration, small dose group TP is a little less than matched group (P＜0.01), heavy dose of group Urea and Glu are all a little more than matched group (P＜0.05), but think to tie up to fluctuation in normal range, do not have a toxicology meaning; Other project measured value of each administration group and the equal nonsignificance of matched group difference.After 2 weeks of drug withdrawal, whole project measured values of each administration group and the equal nonsignificance of matched group difference.
4. exponential measurement results of organ weights such as the heart, lung, liver,spleen,kidney, uterus, testis: after 2 weeks of administration, the organ index of each administration group is all in normal range, with the equal nonsignificance of matched group difference.After 2 weeks of drug withdrawal, the index and spleen index of heavy dose of group is slightly larger than matched group, and difference significance, but still in range of normal value is not thought and had a toxicology meaning; Other organ index of each administration group is all in normal range, with the equal nonsignificance of matched group difference.
Administration 2 week back and cut open after 2 weeks of drug withdrawal and get the main organs of respectively organizing rat and do perusal, there is no tangible morphological change.Administration is cutd open the main organs of getting high dose group and control rats and is done histological examination after 2 weeks, as seen hepatocyte diffusivity or kitchen range shape steatosis all appear in high dose group 12 examples and matched group 8 routine liver specimens, be thought of as in the feedstuff due to plant fat too high levels or the other factors, think and be subjected to the reagent thing irrelevant; Acute pulmonary abscess and interstitial pneumonia appear in two groups individual animal, and these pathological changes are the common idiopathy of animal, and incidence rate is low, irregularities, and group difference is not remarkable, is subjected to due to the reagent thing so think non-.All the other internal organs there is no tangible Histological change.
[conclusion]
The rat long term toxicity test is the result show, by the dosage of crude drug 45.0g/kg, 22.5g/kg, 11.25g/kg respectively the continuous irrigation stomach give 2 weeks of WANTONG YANKANG PIAN crude drug extractum (daily dose is in 100 times, 50 times, 25 times of clinical plan consumption), as seen the leukocyte count that the platelet count of each dosage group slightly increases and 45.0g/kg organizes slightly increases, do not see tangible toxic reaction to occur that drug withdrawal is observed and also do not seen in 2 weeks and significantly delay toxic reaction.It is safe by drafting approach, dosage, the course of treatment in clinical practice that prompting is subjected to reagent thing WANTONG YANKANG PIAN.
Two, product antibacterial tests report of the present invention (by certain provincial institute for drug control test)
Staphylococcus aureus, staphylococcus epidermidis, beta hemolytic streptococcus, bacillus pyocyaneus, Candida albicans drug sensitivity test are carried out to product of the present invention in the institute for drug control, and result of the test is reported as follows:
1, test method and result: it is an amount of to get this product, make 1: 10 test liquid with fluid medium, be diluted to 1: 20 successively again, 1: 40,1: 80,1: 160,1: 320,1: 1280 various diluted concentration, the packing small test tube, every pipe 1ml, drip the staphylococcus aureus of cultivating 24 hours through 37 ℃ respectively, staphylococcus epidermidis, beta hemolytic streptococcus, 1: 1000 diluent 0.05ml of bacillus pyocyaneus and Candida albicans (30 ℃) bacterium liquid, shake up, cultivated 24 hours respectively at 37 ℃ and 30 ℃, each tube culture is inoculated in the manitol salt agar flat board respectively, the plain agar flat board, blood agar plate, hexadecane trimethyl amine bromide agar plate, the mold agar flat board, cultivated 24 hours in said temperature, observation has or not bacterial growth again.
2, conclusion: 1: 640 dilution factor of this product has inhibitory action to staphylococcus aureus, dilution factor had inhibitory action to staphylococcus epidermidis in 1: 80, dilution factor had inhibitory action to bacillus pyocyaneus in 1: 40, and dilution factor had inhibitory action to Candida albicans, beta hemolytic streptococcus in 1: 10.
Three, product pharmacodynamics test report of the present invention
The summary pharmacodynamics test shows that product crude drug extractum of the present invention causes the Hep-2 cytopathy to Coxsackie B virus 3, B5 all certain inhibitory action; Gastric infusion has certain inhibitory action to the hypertrophy of the subcutaneous granuloma induced by implantation of cotton pellets of mice; The Turnover of Mouse Peritoneal Macrophages phagocytic function is not had obvious influence, but generation of CRBC inducing mouse serum hemolysin and the lymphocyte transformation of PHA inducing mouse are all had facilitation.
[test objective]
Be subjected to the reagent thing to Coxsackie virus, chronic inflammatory disease and Immune Effects by the animal experiment observation, the effectiveness of this product made preliminary assessment, for clinical foundation that provides is provided for it.
[being subjected to the reagent thing]
Product of the present invention is the Chinese medicine preparation of Wantong Pharmaceutical Co., Ltd., Guangxi's development.Every of this product contains crude drug 3g, and clinical consumption is 3 of each clothes, and 3 times on the one, 5 days courses of treatment.Being subjected to the reagent thing is the dense thick extractum of this product crude drug, and every g extractum contains crude drug 11g, and lot number 010308 is provided by research institute.The medicinal liquid that will be subjected to the reagent thing to be made into desired concn with deionized water during test supplies rat oral gavage (ig) administration, and each dosage group administration volume is equated.
[test method and result]
The influence test of 1 pair of Coxsackie virus cytopathic effect
[1]
The Hep-2 passage cell is inoculated on the trace Tissue Culture Plate of 96 holes, puts 37 ℃ of 5%CO
2In the incubator, grow up to monolayer after, medicinal liquid is diluted to variable concentrations, add in the cell, the nontoxic boundary of obtaining the medicinal liquid pair cell is crude drug 1.1mg/ml.Adopt two kinds of administering modes during test: administration during cell infection virus, virus contact medicine (2h) postoperative infection cell.Each dilution factor 3 hole, other establishes the contrast of virazole positive drug, virus control, normal cell contrast, diluent contrast, puts 37 ℃ of 5%CO
2In the incubator, every day is the observation of cell pathological changes under inverted microscope, continuous 1～4 day.
WANTONG YANKANG PIAN is to Coxsackie B virus
3, B
5Causing the Hep-2 cytopathy all has certain inhibitory action, the active drug concentration 〉=0.55mg/ml of administration during cell infection virus, the active drug concentration 〉=0.14mg/ml of virus contact medicine postoperative infection cell.
2 pairs of outgrowth influence tests of the subcutaneous granuloma induced by implantation of cotton pellets of mice
[2]
Get 60 of ♂ mices, body weight 21～24g, the anesthesia of every Mus ip pentobarbital sodium, the sterile working will sterilize, and to imbed the back subcutaneous for dry cotton ball.The postoperative mice is divided 5 groups at random, and three groups are given WANTONG YANKANG PIAN crude drug extractum, and positive controls is given prednisone, and the blank group is given ordinary water, and dosage sees Table 2, and the administration volume is 20ml/kg, and every day, ig was administered once, continuous 7 days.Put to death mice next day after the last administration, cuts open and get granuloma induced by implantation of cotton pellets, and in 90 ℃ of oven dry 1 hour, scales/electronic balance weighing deducted former dry cotton ball and heavily is the granuloma net weight, compares t check between the work group with the ordinary water group.The granuloma weight of three dosage groups of WANTONG YANKANG PIAN is all less than the ordinary water group, and heavy dose is organized the difference significance.Prompting this product has certain inhibitory action to the hypertrophy of the subcutaneous granuloma induced by implantation of cotton pellets of mice.
The influence test of 3 pairs of Turnover of Mouse Peritoneal Macrophages phagocytic functions
[3]
Get 50 of mices, ♀ ♂ half and half, body weight 18～21g divides 5 groups at random, three groups are given WANTONG YANKANG PIAN crude drug extractum, and positive controls is given cyclophosphamide, and the blank group is given ordinary water, and dosage sees Table 3, the administration volume is 20ml/kg, and every day, ig was administered once, continuous 7 days.After the last administration 1 hour, the equal ip 5%CRBC of each Mus 0.5ml put to death mice in 1 hour after giving CRBC, and ip normal saline 2ml, gently rub and draw peritoneal irrigation liquid behind the abdominal part and divide and drip two microscope slides, in 37 ℃ of incubation 30min, float the not cell of paster with normal saline, dry, fixing, a Ji-Wright's staining, the oil mirror is 100 macrophages of counting down, calculate phagocytic percentage and phagocytic index, compare, t check between the work group with the ordinary water group.The phagocytic percentage of each dosage of WANTONG YANKANG PIAN and phagocytic index are all close with the ordinary water group, though middle dosage group difference significance, composite score judges, think that this product do not have obvious influence to the Turnover of Mouse Peritoneal Macrophages phagocytic function.
4 pairs of influence tests that CRBC inducing mouse serum hemolysin generates
[4]
Get 50 of mices, ♀ ♂ half and half, body weight 18～22g, grouping and administration were with " test 3 ", continuous 7 days.Each Mus all did CRBC immunity (ip 5%CRBC 0.2ml) on 1.50min after the last administration plucks the eyeball blood sampling, with normal saline serum is diluted 100 times.Get dilute serum 1ml, add 5%CRBC and 10% each 0.5ml of GPC serum, put behind 37 ℃ of incubation 30min i.e. cessation reaction in ice bath, get supernatant after centrifugal to survey its OD value in 546nm wavelength place, with its haemolysis degree of OD value representation, reflect the level of serum hemolysin indirectly.Compare t check between the work group with the ordinary water group.The OD value of three dosage groups of WANTONG YANKANG PIAN is all greater than the ordinary water group, and heavy dose is organized the difference significance.Prompting this product has certain facilitation to the generation of CRBC inducing mouse serum hemolysin.
5 pairs of influence tests that mice PHA induction of lymphocyte transforms
[5]
Get 50 of ♂ mices, body weight 20～23g, grouping and administration were with " test 3 ", continuous 8 days.In the equal im PHA of each Mus 5mg/kg on the 1st, continuous 7 days.After the last administration 1 hour, each Mus is all cut tail blood sampling smear, dries, and was fixing, and Wright's staining, oily mirror be 100 lymphocytes of counting down, record lymphoblast number, with the ordinary water group relatively, t check between the work group.The lymphoblast rate of three dosage groups of WANTONG YANKANG PIAN is all greater than the ordinary water group, and big or middle dosage group difference has highly significant, remarkable meaning.Lymphocyte transformation has certain facilitation to prompting this product to the PHA inducing mouse.
[conclusion]
To sum up result of the test shows, WANTONG YANKANG PIAN crude drug extractum is to Coxsackie B virus
3, B
5Causing the Hep-2 cytopathy all has certain inhibitory action, the active drug concentration 〉=0.55mg/ml of administration during cell infection virus, the active drug concentration 〉=0.14mg/ml of virus contact medicine postoperative infection cell.With WANTONG YANKANG PIAN crude drug extractum gastric infusion, the hypertrophy of the subcutaneous granuloma induced by implantation of cotton pellets of mice there is certain inhibitory action; The Turnover of Mouse Peritoneal Macrophages phagocytic function is not had obvious influence, but generation of CRBC inducing mouse serum hemolysin and the lymphocyte transformation of PHA inducing mouse are all had facilitation.Prompting this product has anti-Coxsackie virus, anti-chronic inflammatory disease, improves effects such as body fluid and cellular immune function, certain animal experiment foundation is provided for the clinical practice of this product.
Four, product I II clinical trial phase of the present invention is summed up
Product of the present invention is the Chinese medicine preparation that Wantong Pharmaceutical Co., Ltd., Guangxi produces, and has the effect of heat-clearing and toxic substances removing, reducing swelling and alleviating pain, is used for laryngopharynx swelling and pain, acute pharyngitis, laryngitis, tonsillitis, gingivitis, furuncle.In order further to investigate its curative effect, attending scope and untoward reaction, according to " provisions for new drugs approval "
[1], " tcm syndrome standard "
[2], " clinical disease diagnosis according to cure improvement standard "
[3]" new Chinese medicine clinical research guideline "
[4～7], Guangxi Wu Jia provincial hospital has carried out clinical trial year April in March, 1994 to 2000 to WANTONG YANKANG PIAN, and the result is summarized as follows:
Purpose
Investigate the clinical efficacy of product of the present invention treatment accumulated heat in the lung and stomach card (acute pharyngitis, laryngitis, tonsillitis, gingivitis, furuncle) and to the safety of human body.
Object and method
One, case choice criteria:
(1) tcm diagnosis standard (accumulated heat in the lung and stomach card):
1, main symptom: 1. laryngopharynx swelling and pain; 2. gingival swelling and pain; 3. Chuan Jie inflammation is bitterly red and swollen.
2, inferior card: 1. heating; 2. headache; 3. dry pharynx is scorching hot; 4. halitosis; 5. oliguria with reddish urine; 6. constipation with dry stool; 7. pharyngeal hyperemia; 8. vocal congestion; 9. pharyngeal tonsils hyperemia.
(2) Western medicine diagnose standard:
1, acute pharyngitis:
(1) medical history: often suffer from cold, inducements such as tired, excessive, the various physics of tobacco and wine and chemical stimulation.
(2) symptom: onset is more anxious, pharyngeal drying, scorching hot, pain or odynophagia, heating, fear of cold, inappetence, extremities aching pain.
(3) check: pharyngeal mucosa hyperemia, color is scarlet; Pharynx rear wall lymph follicle and lateral pharyngeal band redness, local or have Huang, white secretions to adhere to; Uvula, soft palate edema; Throat swab is cultivated can pathogenic bacterium or feminine gender.
More than possess part or symptomatology, and inspection finding positive sign more than 1 or 1 is arranged, promptly diagnosable.
2, acute laryngitis:
(1) symptom: throat is itched, hypodynia, and throat has foreign body to stop up sense, hoarseness, the effort of speaking, weight person is bordering on and cries out, brings up phlegm when one coughs, but General Symptomies such as weight person's aversion to cold, heating, tired, inappetence.
(2) indirect laryngoscopy: the hyperemia of larynx mucosa diffusivity, swelling, it is the most obvious to detest pleat with vocal cords and meeting Wood-scoop, and the initial stage vocal cords are pale red, crossfade into peony, or ecchymosis occurs, the vocal cords edge thickens, glottic insufficiency during pronunciation, vocal area can be thick secretions and adhere to.
3, acute tonsillitis:
(1) medical history: often suffer from cold, tired, tobacco and wine excessively, malnutrition, the low inferior inducement of passive protective physical fitness.
(2) symptom: it is hurried to fall ill, general malaise, and the shiver with cold heating, fervescence, headache, nape and extremities aching pain, inappetence often has constipation, and pharyngeal pain increases the weight of when swallowing, and in ear pain is often arranged.(3) check: patient's face of acute ill, the buccal flushing, halitosis, thick fur is white, and the tonsil redness has yellow-white pus point in the crypts, can merge in flakes, and shape such as pseudomembrane are easily wiped, mandibular lymph node enlargement and tenderness, the lab testing total white blood cells can increase.
4, marginal gingivitis:
(1) the gingival hemorrhage history is arranged, but must at first get rid of the general hemorrhage.
(2) can look into see tartar is arranged, soft dirt etc. is attached to facing, if use disclosing agent, then as seen bacterial plaque is thereon attached or facing is on and off duty, or local factor such as bad dummy is arranged.
(3) gum edge, gum nipple congestion and edema, gingival sulcus have intensification, color dark red slightly, and that touches is easily hemorrhage.
5, furuncle:
(1) local skin red and swollen heat pain.
(2) symptoms such as heating, xerostomia, dry stool can be arranged.
(3) can there be total white blood cells and neutrophilic granulocyte to increase.
Two, observational technique: adopt Self-control method to observe.
1, observes 300 examples altogether, finish by five tame hospitals.
2, usage and dosage: WANTONG YANKANG PIAN (Film coated tablets), oral, each 2, every day 3 times.3, the course of treatment: 7 days.3, viewing duration need not be treated the other medicines of primary disease.Three, witness light and heavy degree grade scale and scoring method: see Table 1.Table 1 witness light and heavy degree grade scale and scoring method
Slightly (2 minutes) moderate (4 minutes) severe (6 minutes) is had sore throat hypodynia more very, odynophagia is heavy, the dysphagia gingival swelling and pain is confined to a tooth position scope and exceeds a tooth position, red and swollen painful two the tooth positions that exceed, red and swollen painful
When not noting unconsciously the pain pain obviously still can stand pain unbearably, it is little red, hard slightly influence the rest work furuncle furuncle that swells and ache, pain furuncle redness, the obvious furuncle swelling of pain more Shen ， inflammation is red bright
Slight fever, 37.1 ℃～37.5 ℃～38 ℃ 38 ℃～headache of sharp ache heating is light, dull pain appears once in a while, be interrupted and occur weighing, the scorching hot dry pharynx of intense splitting headache dry pharynx is light, occur more once in a while, no burning sensation is heavy, there is the low pronunciation of burning sensation hoarseness pronunciation overcast, chap sound is like whispered sound, speech effort halitosis is conscious bitter taste, halitosis other people's audible and the obvious halitosis of obvious halitosis are made us difficult nearly oliguria with reddish urine urine colour cast Huang, amount normal urine yellow skin, the normal oliguria color depth Huang of amount, the obviously deficient constipation of urine amount is hard partially, and every day, 1 scleroma was difficult to resolve, and every day, 2～3 scleromas were difficult to resolve, companion's abdominal distention, 3
1 pharyngeal hyperemia of stool is limited to pharynx rear wall pharynx rear wall more than day, lateral pharyngeal band pharynx rear wall, funiculus lateralis and uvula vocal congestion vocal cords pale red, little swollen vocal cords are little swollen, the congested swelling of the congested vocal cords diffusivity of diffusivity
The congested pharyngeal tonsils hyperemia of the congested pharyngeal tonsils of swelling glottic insufficiency pharyngeal tonsils has pus point pharyngeal tonsils redness, the secretions slabbing
Four, efficacy assessment standard:
1, recovery from illness: sx in the medication 3 days, symptomatology and sign disappear or basic the disappearance in 7 days, see that the disease integration reduces more than 91%.
2, produce effects: most of sign disappears in the medication 7 days, sees that the disease integration reduces 61～90%.
3, effective: 7 days inner signs of dividing of medication disappear, and see that the disease integration reduces 31～60%.
4, invalid: as not reach above standard person.
Five, statistical procedures: ranked data Ridit analytic process, enumeration data X
2Check, measurement data is checked with t.
Six: the result:
(1) efficacy analysis:
1, total effects distributes: table 2 total effects distributes
Example number clinical recovery (%) produce effects (%) effectively total effectively (%) treatment of (%) invalid (%) organizes 300 106 (35.5) 83 (27.7) 87 (29.0) 24 (8.0) 276 (92.0)
2, the different sick curative effects of planting compare: the different sick curative effects of planting of table 3 relatively (are organized the Ridit analysis) more
Example number clinical recovery (%) produce effects (%) effectively total effectively (%) acpuei pharyngitis 60 13 (21.7) 20 (33.3) 23 (38.3) 4 (6.7) 56 (93.3) acute laryngitis of (%) invalid (%) 60 23 (38.3) 11 (18.3) 21 (35.0) 5 (8.3) 55 (91.7) tonsillitis 60 24 (40.0) 19 (31.7) 13 (21.7) 4 (6.7) 56 (93.3) gingivitis 60 22 (36.7) 17 (28.3) 15 (25.0) 6 (10.0) 54 (90.0) sore furuncles 60 24 (40.0) 16 (26.7) 15 (25.0) 5 (8.3) 55 (91.7) is annotated: RPharyngitisValue=0.5633; R
LaryngitisValue=0.5091; R
TonsillitisValue=0.4583; R
GingivitisValue=0.4938;
R
FuruncleValue=0.4755; X
2=4.65, X
2 0.05 (4)=9.49, P＞0.05.
3, treatment back clinical symptoms curative effect distributes: table 4 treatment back clinical symptoms curative effect relatively
The example number improves 3 grades and improves 2 grades and improve 1 grade without improving scorching hot 216 35 82 83 16 hoarsenesses of 172 13 90 63 6 swelling and aching of gum, 82 16 26 33 7 sore furuncles pain, 61 26 20 10 5 heatings, the 127 12 40 75 0 headache 133 18 41 74 0 dry throats 82 12 16 41 13 halitosis 161 26 64 43 28 scanty drak urines 195 31 53 82 29 dry and hard excrements 179 36 64 53 26 pharyngeal hyperemias 182 25 73 56 28 injection of vocal cord 60 16 32 17 larynxs nuclear congested 64 3 36 13 12 of having sore throat
(2) safety detects:
1, routine blood test: detected 299 examples before the treatment, wherein, there is no unusual in the 280 example treatment back checks of normal range.
2, routine urinalysis: detected 291 examples before the treatment, wherein, there is no unusual in the 283 example treatment back checks of normal range.
3, stool routine examination: detected 291 examples before the treatment, wherein, there is no unusual in the 291 example treatment back checks of normal range.
4, electrocardiogram: detected 276 examples before the treatment, wherein, there is no unusual in the 267 example treatment back checks of normal range.
5, ALT: detected 271 examples before the treatment, wherein, there is no unusual in the 263 example treatment back checks of normal range.
6, BCr: detected 273 examples before the treatment, wherein, there is no unusual in the 271 example treatment back checks of normal range.
7, BUN: detected 273 examples before the treatment, wherein, there is no unusual in the 269 example treatment back checks of normal range.
(3) untoward reaction is observed: the untoward reaction symptom all do not occur after two groups of medications.
Discuss and conclusion
1, this group patient cure rate, obvious effective rate, effective percentage are respectively 35.3%, 27.7%, 29%, and total effective rate is 92%.The prompting WANTONG YANKANG PIAN has clinical efficacy preferably to acute pharyngitis, acute laryngitis, acute tonsillitis, gingivitis, furuncle.
2, the different sick efficacy results of planting show that WANTONG YANKANG PIAN all has clinical efficacy preferably to acute pharyngitis, acute laryngitis, acute tonsillitis, gingivitis and furuncle, and difference does not have the significance meaning.
3, the clinical symptoms efficacy result shows, symptom, signs such as treatment back patient's laryngopharynx swelling and pain, gingival swelling and pain, furuncle swell and ache, generate heat, headache, dry pharynx are scorching hot, halitosis, oliguria with reddish urine, constipation with dry stool, pharyngeal hyperemia, vocal congestion, pharyngeal tonsils hyperemia all have clear improvement.The prompting WANTONG YANKANG PIAN can be improved the clinical symptoms of acute pharyngitis, acute laryngitis, acute tonsillitis, gingivitis, furuncle preferably.
4, this research has been carried out blood, urine, just routine examination to 299 routine patients, and the heart, liver, kidney function test are not all found obvious adverse reaction and toxic and side effects.Prompting this product is clinical safe and reliable.
Model case
Beautiful certain, the man 23 years old, lives Tangshan road, Nanning city company dormitory, because of pharyngalgia was gone to see a doctor in 2 days.Private prosecution before 2 days cause daily life pharyngalgia appears accidentally, with fever with chills, the headache, dry pharynx is scorching hot, hoarseness, halitosis, oliguria with reddish urine, constipation with dry stool.Check: 37.8 ℃ of T, R 20 times/minute, P 88 times/minute.Pharynx rear wall, funiculus lateralis and the obvious congestion and edema of uvula, the congested swelling of vocal cords diffusivity.The rhythm of the heart is whole, and heart rate 88 times/minute is not heard the cardiac valve pathologic murmur.Two pulmonary respiration sounds are clear, do not hear dried moist rale.Abdomen is flat soft, and no tenderness rebound tenderness is not touched under the liver spleen rib, and borborygmus is normal.Red tongue, thin fur, floating and rapid pulse.Routine blood test: HB 116g/L, RBC 4.05 * 10
12/ L, WBC 10.0 * 10
9/ L.Routine urinalysis (-), stool routine examination (-).Electrocardioscopy is no abnormal.ALT＜10u/L, BUN 5.6mmol/L, BCr 96 μ mol/L, tcm diagnosis: acute inflammation of the throat (accumulated heat in the lung and stomach card).Western medicine diagnose: acute pharyngitis.Give Chinese medicine preparation of the present invention (Film coated tablets) treatment, oral, each 2, every day 3 times, logotype 7 days.The after heat in 3 days of taking medicine is moved back, 36.5 ℃ of body temperature, and no headache, the scorching hot phenomenon of pharyngalgia dry pharynx, hoarseness obviously alleviates.Pharyngalgia dry pharynx and hoarseness disappear after 5 days, and pharyngeal hyperemia obviously alleviates.Pharyngeal hyperemia and vocal congestion disappear after 7 days, and defecation is normal.Routine blood test: HB 112g/L, RBC 4.0 * 10
12/ L, WBC 8.3 * 10
9/ L.Routine urinalysis (-), stool routine examination (-).Electrocardiogram and liver function, kidney function test are all no abnormal.Efficacy evaluation: recovery from illness.
Claims (6)
1, a kind of Chinese medicine preparation for the treatment of upper respiratory system disease and skin infection and hemorrhoid is characterized in that it comprises Radix Picriae felterrae and Herba Sarcandrae, and the composition and the weight content of its primary raw material are as follows:
Radix Picriae felterrae 5～95%
Herba Sarcandrae 95～5%
Described Chinese medicine preparation comprises tablet, capsule, pill, granule, oral liquid.
2, the preparation method of the Chinese medicine preparation of the described treatment upper respiratory system disease of a kind of claim 1 and skin infection and hemorrhoid, the preparation method that it is characterized in that this Chinese medicine preparation tablet is: Radix Picriae felterrae and Herba Sarcandrae are washed respectively cut, fragmentation, add 6～10 times of water heated and boiled 1.5～3 hours by the per kilogram medicine, take out filtrate, filter, medicinal residues added water boil 1～2 hour again, merging filtrate, concentrate, add ethanol precipitation, supernatant reclaims ethanol, concentrate, make granule with an amount of filler, disintegrating agent, tabletting, coating obtains product.
3, the Chinese medicine preparation of the described treatment upper respiratory system disease of a kind of claim 1 and skin infection and hemorrhoid, the preparation method that it is characterized in that this Chinese medicine preparation capsule is: Radix Picriae felterrae and Herba Sarcandrae are washed respectively cut, fragmentation, add 6～10 times of water heated and boiled 1.5～3 hours by the per kilogram medicine, take out filtrate, filter, medicinal residues added water boil 1～2 hour again, merging filtrate concentrates, and adds ethanol precipitation, supernatant reclaims ethanol, concentrate, in concentrated solution, add filler, disintegrating agent and fluidizer, and make it dry, in incapsulating then, obtain product.
4, the Chinese medicine preparation of the described treatment upper respiratory system disease of a kind of claim 1 and skin infection and hemorrhoid, the preparation method that it is characterized in that this Chinese medicine preparation pill is: Radix Picriae felterrae and Herba Sarcandrae are washed respectively cut, fragmentation, add 6～10 times of water heated and boiled 1.5～3 hours by the per kilogram medicine, take out filtrate, filter, medicinal residues added water boil 1～2 hour again, and merging filtrate concentrates, add ethanol precipitation, supernatant reclaims ethanol, concentrates, and adds filler in the concentrated solution that obtains, stir, make pill, and make it dry, coating promptly obtains product.
5, the Chinese medicine preparation of treatment upper respiratory system disease according to claim 1 and skin infection and hemorrhoid, the preparation method that it is characterized in that this Chinese medicine preparation granule is: Radix Picriae felterrae and Herba Sarcandrae are washed respectively cut, fragmentation, add 6～10 times of water heated and boiled 1.5～3 hours by the per kilogram medicine, take out filtrate, filter, medicinal residues added water boil 1～2 hour again, merging filtrate concentrates, and adds ethanol precipitation, supernatant reclaims ethanol, concentrate, in the concentrated solution that above-mentioned production obtains, add filler, make granule, make it dry, obtain product.
6, the Chinese medicine preparation of treatment upper respiratory system disease according to claim 1 and skin infection and hemorrhoid, the preparation method that it is characterized in that this Chinese medicine preparation oral liquid is: Radix Picriae felterrae and Herba Sarcandrae are washed respectively cut, fragmentation, add 6～10 times of water heated and boiled 1.5～3 hours by the per kilogram medicine, take out filtrate, filter, medicinal residues added water boil 1～2 hour again, merging filtrate, concentrate, add ethanol precipitation, supernatant reclaims ethanol, concentrates, in the concentrated solution that above-mentioned production obtains, add sucrose or Mel, Radix Glycyrrhizae solution, obtain product.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103948736A (en) * | 2014-04-14 | 2014-07-30 | 张树球 | Chinese herbal medicine compound preparation for treating liver cancer and production method thereof |
| CN105770454A (en) * | 2016-04-29 | 2016-07-20 | 徐玉梅 | Traditional Chinese medicine preparation for nursing infectious respiratory tract inflammation and preparation method thereof |
-
2003
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103948736A (en) * | 2014-04-14 | 2014-07-30 | 张树球 | Chinese herbal medicine compound preparation for treating liver cancer and production method thereof |
| CN105770454A (en) * | 2016-04-29 | 2016-07-20 | 徐玉梅 | Traditional Chinese medicine preparation for nursing infectious respiratory tract inflammation and preparation method thereof |
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