CN1431508A - 检测单纯疱疹病毒II型抗体IgG的免疫胶体金试剂及制备方法 - Google Patents
检测单纯疱疹病毒II型抗体IgG的免疫胶体金试剂及制备方法 Download PDFInfo
- Publication number
- CN1431508A CN1431508A CN 03115139 CN03115139A CN1431508A CN 1431508 A CN1431508 A CN 1431508A CN 03115139 CN03115139 CN 03115139 CN 03115139 A CN03115139 A CN 03115139A CN 1431508 A CN1431508 A CN 1431508A
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- Prior art keywords
- rabbit
- albumin
- pad
- preparation
- herpes simplex
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 29
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title claims description 22
- 241000701074 Human alphaherpesvirus 2 Species 0.000 title abstract 3
- 241000283973 Oryctolagus cuniculus Species 0.000 claims abstract description 29
- 239000000020 Nitrocellulose Substances 0.000 claims abstract description 15
- 238000001514 detection method Methods 0.000 claims abstract description 15
- 229920001220 nitrocellulos Polymers 0.000 claims abstract description 15
- 241000283707 Capra Species 0.000 claims abstract description 14
- 241000287828 Gallus gallus Species 0.000 claims abstract description 9
- 239000000084 colloidal system Substances 0.000 claims description 22
- 239000010931 gold Substances 0.000 claims description 22
- 229910052737 gold Inorganic materials 0.000 claims description 22
- 101710153593 Albumin A Proteins 0.000 claims description 20
- 241000700584 Simplexvirus Species 0.000 claims description 17
- 239000000427 antigen Substances 0.000 claims description 9
- 102000036639 antigens Human genes 0.000 claims description 9
- 108091007433 antigens Proteins 0.000 claims description 9
- 230000000274 adsorptive effect Effects 0.000 claims description 6
- 230000003053 immunization Effects 0.000 claims description 6
- 230000002829 reductive effect Effects 0.000 claims description 5
- 208000009889 Herpes Simplex Diseases 0.000 claims description 3
- 230000003602 anti-herpes Effects 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 239000001509 sodium citrate Substances 0.000 claims description 3
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical class [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 claims description 3
- 235000019263 trisodium citrate Nutrition 0.000 claims description 3
- FDWREHZXQUYJFJ-UHFFFAOYSA-M gold monochloride Chemical compound [Cl-].[Au+] FDWREHZXQUYJFJ-UHFFFAOYSA-M 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 12
- 238000010521 absorption reaction Methods 0.000 abstract description 4
- 239000012528 membrane Substances 0.000 abstract 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- 101710181478 Envelope glycoprotein GP350 Proteins 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 7
- 230000002950 deficient Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 238000005138 cryopreservation Methods 0.000 description 3
- 238000001262 western blot Methods 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 239000005030 aluminium foil Substances 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 238000005325 percolation Methods 0.000 description 2
- 238000003127 radioimmunoassay Methods 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- -1 cleaning Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
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- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Description
Claims (2)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 03115139 CN1431508A (zh) | 2003-01-24 | 2003-01-24 | 检测单纯疱疹病毒II型抗体IgG的免疫胶体金试剂及制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN 03115139 CN1431508A (zh) | 2003-01-24 | 2003-01-24 | 检测单纯疱疹病毒II型抗体IgG的免疫胶体金试剂及制备方法 |
Publications (1)
Publication Number | Publication Date |
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CN1431508A true CN1431508A (zh) | 2003-07-23 |
Family
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Family Applications (1)
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CN 03115139 Pending CN1431508A (zh) | 2003-01-24 | 2003-01-24 | 检测单纯疱疹病毒II型抗体IgG的免疫胶体金试剂及制备方法 |
Country Status (1)
Country | Link |
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CN (1) | CN1431508A (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109283334A (zh) * | 2018-09-30 | 2019-01-29 | 潍坊市康华生物技术有限公司 | 一种检测单纯疱疹病毒II型IgG抗体的重组抗原组合物及其试剂盒 |
-
2003
- 2003-01-24 CN CN 03115139 patent/CN1431508A/zh active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109283334A (zh) * | 2018-09-30 | 2019-01-29 | 潍坊市康华生物技术有限公司 | 一种检测单纯疱疹病毒II型IgG抗体的重组抗原组合物及其试剂盒 |
CN109283334B (zh) * | 2018-09-30 | 2021-09-07 | 山东硕景生物科技有限公司 | 一种检测单纯疱疹病毒II型IgG抗体的重组抗原组合物及其试剂盒 |
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ASS | Succession or assignment of patent right |
Owner name: BODUN BIOTEST TECHNOLOGY(HANGZHOU) CO., LTD Free format text: FORMER OWNER: HUZHOU RUIZE BIOTECHNOLOGY CO., LTD Effective date: 20040325 |
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C41 | Transfer of patent application or patent right or utility model | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20040325 Address after: 311215 four road, Xiaoshan economic and Technological Development Zone, Hangzhou, Zhejiang Applicant after: Bioden Inspection Biotech (Hangzhou) Co., Ltd. Address before: 313000 Zhejiang Province, Huzhou City Longxi Road No. 208 Applicant before: Ruize Biotechnology Co., Ltd., Huzhou |
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C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
WD01 | Invention patent application deemed withdrawn after publication |