CN1428106A - Pinenut polypeptide beverage and its preparation method - Google Patents
Pinenut polypeptide beverage and its preparation method Download PDFInfo
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- CN1428106A CN1428106A CN01138933.8A CN01138933A CN1428106A CN 1428106 A CN1428106 A CN 1428106A CN 01138933 A CN01138933 A CN 01138933A CN 1428106 A CN1428106 A CN 1428106A
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Abstract
The present invention relates to a pinenut polypeptide beverage, in which it contains the following components: acid-soluble peptide yield is greater than or equal to 25%, protein is greater than or equal to 0.5%, fat is greater than or equal to 0.6%, soluble solids are greater than or equal to 6% and the rest is water. Its preparation method includes the following steps: soaking the pinenut in sodium hydroxide solution whose concentration is 0.2% and temp. is 80 deg.C for 4 min.; mixing the shelled pinenut and water according to the ratio of 1:15 and grinding them to make pulp; enzymolyzing said pulp whose pH value is 7.5-8 at 30-50 deg.C for 1-2 hr, by using proteinase; inactivating enzyme for 10-15 min. at 80-90 deg.C; adding white granulated sugar and two emulsifiers into the enzymolyzed product; diluting, volume-fixing and sieving with 300 meshes.
Description
Technical field the present invention relates to a kind of drink, particularly is the drink of primary raw material with the pinenut.
The background technology pinenut is a kind of food that contains multiple nutritional components.Eat for convenience and store, people have designed the scheme that is processed into beverage, as on December 29th, 1993 Gazette of Patent for Invention disclosed a kind of＜all-natural pine nut milk and pinenut breast preparation method (application number 93103388.8) it be to adopt pinenut as primary raw material, add additive and water such as sugar, salt, emulsifying agent, stabilizing agent, antioxidant, fumet then again, can obtain all-natural pine nut milk through main programs such as the enzyme that goes out, peeling, defibrination, ultra-fine high pressure homogenate, sterilizations.Its weak point is that the protein of pinenut is to exist with big molecular forms, is difficult for being absorbed by human body.
Summary of the invention the present invention is to be primary raw material with the pinenut, through a kind of polypeptide beverage that enzymolysis is made, protein content in this drink 〉=0.5%, solubility in acid peptide yield 〉=25%, fat content 〉=0.6%, soluble solid content 〉=6%, remaining amount are water.
Preparation method's technological process of drink of the present invention is referring to Fig. 1, and concrete operations are as follows:
One, primary raw material and processing: main material of the present invention is the pinenut that shells.
1, selection: choose and remove the rotten pinenut that shells that goes rotten.
2, preliminary treatment: with will shell the pinenut of underwear of 0.2% sodium hydroxide solution is to soak 4 minutes under 80 ℃ the condition in temperature, and water is rinsed well then.
3, defibrination: adopt one of following method to carry out defibrination, control pinenut: water=1: 15 (weight ratio)
1. adopt conventional fiberizer, pinenut is mixed with 1: 5 weight ratio with water, use paste mill grinding, slurries are crossed 100 mesh sieves, the water of equivalent carries out defibrination second time when getting filter residue and adding with defibrination for the first time, and slurries are crossed 100 mesh sieves, get filter residue add again with the first time during defibrination water of equivalent carry out defibrination for the third time, slurries are crossed 100 mesh sieves, and it is stand-by to merge three defibrination gained filtrates.
2. use three roller stone mills that pinenut is levigate, add then pinenut and water with 1: 15 weight ratio again by the colloid mill defibrination, then slurries are crossed 100 mesh sieves, it is stand-by to get filtrate.
Two, auxiliary material and processing: it is that pinenut is made raw material that the present invention can all adopt primary raw material, but because the fatty amount of pinenut is higher, therefore adding too much pinenut easily makes fat floating, increases cost simultaneously again.For guaranteeing protein content in the finished product, can adopt casein, collagen and soybean to replenish as auxiliary material.Wherein casein is mammiferous dairy products, and it contains rich in protein, and now as the important source of physiologically active peptide, it can become various immune peptides through enzyme hydrolysis, and as the antithrombotic peptide, the mineral element binding peptide is CPP (CPP).Collagen is the main component of animal connective tissue, its enzymolysis is become collagen polypeptide, the molecular weight ratio collagen is much smaller, being beneficial to human consumption absorbs, and it has functions such as fine solubility, absorbability, foaming characteristic, water imbibition, not only can be used as foodstuff base material but also can be used as NMF, emulsion stabilizer and blowing agent.When replenishing casein or collagen, need to take out a certain amount of pinenut filtrate, heating makes temperature to 50 ℃, then added casein or collagen is beaten repeatedly, is mixed with mixer, last and filtrate merging.The method of replenishing soybean is: earlier soybean is handled, its method is as follows: soybean is through impurity elimination, places 95 ℃ of water enzyme that carries out going out in 5～20 minutes to handle.Utilize peeling machine that skin of beancurd is taken off, slough lipoxidase contained in the skin of beancurd simultaneously, remove beany flavor and bitter taste.Place the water of 3 times of soybean weight to soak then, fully hydration.The adding soybean is measured 6 times water mill slurry after pulling soybean out, crosses 150 eye mesh screens.Adopt 19.6～26.7KPa vacuum to carry out deodorization, moment boils off bad smell, and the product that obtains is the soybean slurries, wherein contains abundant soybean protein.
Three, enzymolysis
1, the enzyme that enzymolysis adopted be protease as
Bromelain (500,000 units/gram enzymes are lived)
As1398 neutral proteinase (180,000 units/gram enzymes are lived)
COROLASE PN-L (200,000 units/gram enzymes are lived)
2, the consumption of enzyme is represented with following formula
Wherein: W
Enzyme---enzyme dosage
W
Former---protein quality in the raw material
3, the consumption of raw material can adopt following raw material for guaranteeing protein content 〉=0.5% in the finished product
1. raw material all is the pinenut that shells: the pinenut consumption calculates with following formula
2. raw material is pinenut and casein mixture: the pinenut consumption that shells is 1～2% * finished weight, casein consumption 〉=0.4% * finished weight.
3. raw material is pinenut and collagen mixture: the pinenut consumption that shells is 1～2% * finished weight, collagen consumption 〉=0.4% * finished weight.
4. raw material is pinenut and soybean mixture, and the pinenut consumption that shells is 1～2% * finished weight, soybean consumption 〉=1.5～2% * finished weight.
4, enzymatic hydrolysis condition
30～50 ℃ of temperature, 1～2 hour time, pH value 7.5～8
5, the enzyme that goes out: with the product behind the enzymolysis 80～90 ℃ of insulations 10～15 minutes
Four, allotment
1, allotment:, in enzymolysis product, add 4～6% white granulated sugars and 0.1～0.3% molecule distillating monoglyceride and 0.1～0.3% octadecyl sodium lactate with respect to finished weight for adjusting beverage mouthfeel.
2, dilution, filtration: thin up is crossed 300 mesh sieves then to the concentration of predesignating in material.
3, homogeneous: the material after will filtering is heated to 62～80 ℃ temperature, twice of 25～30MPa homogeneous.
4, sterilization can: the material that will cross through homogeneous was through 137～140 ℃ of ultra high temperature sterilizations 3～8 seconds, and outlet temperature is controlled at 80～90 ℃.Can be adopted dual mode, and a kind of is the material behind the ultra high temperature sterilization to be squeezed into the sterile filling production line with pump carry out can, and what obtain promptly is finished product.Another kind of mode is that the material hot filling is gone in the heatproof container, and again through 121 ℃ of temperature sterilizations 30 minutes, what obtain promptly is finished product after sealing.
Pinenut polypeptide beverage is to decompose the product that makes through enzyme by pure pinenut albumen, pinenut albumen and casein, pinenut albumen and collagen, pinenut albumen and soybean protein.Its many peptide species molecular mixing after by proteolysis is formed, and its nutritive value equates with the protein of constitutive material.But compare just like feature with traditional pinenut drink:
1. Pinenut polypeptide beverage is is easily digested and assimilated;
2. can provide energy to body rapidly;
3. there is not protein denaturation, no residue, molecular weight is little, and is soluble in water;
4. do not produce precipitation under acid condition, solution viscosity is little, is heated not solidify;
5. the polypeptide mass-energy in the Pinenut polypeptide beverage combines with cholic acid in the body, has functions such as the human serum of reduction cholesterol, hypotensive and fat-reducing.
Specific embodiment example 1
Remove 3.5 kilograms of shell pinenuts (protein 〉=15%), remove underwear and the rotten pinenut that goes rotten, put into 0.2% sodium hydroxide solution then, soaked 4 minutes in 80 ℃ of temperature, alkali lye was not advisable to have material.Wash pinenut with running water, then the water of pinenut with 17.5 kilograms is mixed, use paste mill grinding, slurries are crossed 100 mesh sieves.In filter residue, add 17.5 kg water again and mix, carry out the defibrination second time, slurries are crossed 100 mesh sieves.Get filter residue and add the mixing of 17.5 kg water again, carry out defibrination for the third time, slurries are crossed 100 mesh sieves.Merging defibrination gained filtrate three times, add 2.6 gram bromelains in above-mentioned filtrate, is 8 at pH value, enzymolysis 2 hours when temperature is 30 ℃, then at 15 minutes enzymes that go out of 80 ℃ of temperature insulations.Add 4 kilograms of white granulated sugars and 0.1 kilogram of molecule distillating monoglyceride emulsifying agent and 0.3 kilogram of above-mentioned product of octadecyl sodium lactate emulsion thin up in above-mentioned product, making gross weight is double centner.Cross 300 mesh sieve fine filterings then, get filtrate and be heated to 62 ℃ of temperature, twice of 24.5MPa homogeneous, 137 ℃ of 8 seconds of UHTS, the material outlet temperature of superhigh temperature disinfecting machine is 80 ℃, pours in the vial more again, again through 121 ℃ of temperature sterilizations 30 minutes, what obtain is finished product, wherein contains 0.53% protein, 26.7% solubility in acid peptide yield, 2.1% fat, 7% soluble solid, and remaining amount is a water.Example 2
Repeat the operation of example 1,1 kilogram of the pinenut of wherein shelling adds 5 kilograms in water at every turn during defibrination, obtain defibrination filtrate three times.Take out part filtrate and be heated to 50 ℃ of temperature, add 0.4 kilogram of casein, fully stir, incorporate into again in the former slurry, in above-mentioned mixed liquor, add 5 gram As1398 neutral proteinases, be 7.5 at pH value, enzymolysis 1 hour during 50 ℃ of temperature, then at 10 minutes enzymes that go out of 90 ℃ of temperature insulations.In above-mentioned product, add 6 kilograms of white granulated sugars and 0.3 kilogram of molecule distillating monoglyceride emulsifying agent and 0.1 kilogram of octadecyl sodium lactate emulsifying agent.Thin up control material gross weight is a double centner, cross 300 mesh sieve fine filterings then, get filtrate and be heated to 80 ℃ of temperature homogeneous twice under the 25MPa pressure condition, again in 140 ℃, 3 seconds of ultra high temperature sterilization, when being cooled to 90 ℃ of temperature, squeeze into the sterile filling production line with pump, carry out sterile filling, what obtain is finished product, contain 0.5% protein, 25.11% solubility in acid peptide yield, 0.6% fat, 7% soluble solid in this finished product, remaining amount is a water.Example 3
Repeat the operation of example 2,2 kilograms of the pinenuts of wherein shelling add 10 kilograms in water at every turn during defibrination, merge three times defibrination filtrate.Therefrom take out part filtrate, be heated to 50 ℃ of temperature, add 0.4 kilogram of collagen, stir, mix with pinenut filtrate then.In mixed liquor, add 7 gram As1398 neutral proteinases, pH value is 7.5, temperature is 40 ℃ of enzymolysis 2 hours, then at 83 ℃ of enzymes 15 minutes of going out, in above-mentioned product, add 6 kilograms of white granulated sugars and 0.3 kilogram of molecule distillating monoglyceride and 0.2 kilogram of octadecyl sodium lactate, thin up control material gross weight is a double centner, cross 300 mesh sieve fine filterings then, get filtrate and be heated to 70 ℃, twice of 28MPa homogeneous, again through 138 ℃ of 5 seconds of UHTS, 90 ℃ of outlet temperature controls enter sterile filling and obtain finished product.Contain 0.67% protein, 28.11% solubility in acid peptide yield, 1.15% fat, 8% soluble solid in this finished product, remainder is a water.Example 4
Repeat example 1 operation, wherein pinenut is 2 kilograms, adds 10 kilograms in water during defibrination at every turn, and it is stand-by to merge three defibrination gained filtrates, gets selection 1.5 kilograms of soybean afterwards, places go out enzyme 20 minutes of 95 ℃ of water.Adopt soybean dehulling machine that skin of beancurd is taken off, place 4.5 kilograms water to soak, fully hydration.Add 9 kilograms water mill slurry after pulling out again, and cross 150 eye mesh screens.Adopt 19.6KPa vacuum to carry out deodorization, above-mentioned soybean filtrate and pinenut filtrate are merged, in above-mentioned mixed liquor, add 7.5 gram COROLASE PN-L, at pH value is 8, temperature is 50 ℃ of enzymolysis 1.5 hours, then at go out enzyme 10 minutes of 80 ℃ of temperature, in above-mentioned product, add 4 kilograms of white granulated sugars and 0.2 kilogram of molecule distillating monoglyceride and 0.1 kilogram of octadecyl sodium lactate, thin up control material total amount is a double centner, cross 300 mesh sieve fine filterings then, get filtrate and be heated to 75 ℃, at 30MPa homogeneous twice, again 140 ℃ of 5 seconds of ultra high temperature sterilization, pour into then in the heat-resisting PET bottle, seal.Again through 121 ℃ of temperature sterilizations 30 minutes, what obtain is finished product.Contain 0.93% protein, 32% solubility in acid peptide yield, 1.5% fat, 6.0% soluble solid in this finished product, remaining amount is a water.Example 5
Repeat example 1 operation, wherein pinenut is 1 kilogram, adds 5 kilograms in water during defibrination at every turn, and it is stand-by to merge three defibrination gained filtrates, gets selection 2 kilograms of soybean afterwards, places go out enzyme 5 minutes of 95 ℃ of water.Adopt soybean dehulling machine that skin of beancurd is taken off, place 6 kilograms water to soak, fully hydration.Add 12 kilograms water mill slurry after pulling out again, and cross 150 eye mesh screens.Adopt 26.7KPa vacuum to carry out deodorization, above-mentioned soybean filtrate and pinenut filtrate are merged, in above-mentioned mixed liquor, add 6 gram bromelains, at pH value is 7.8, temperature is 45 ℃ of enzymolysis 2 hours, then at 85 ℃ of enzymes 12 minutes of going out, in above-mentioned product, add 5 kilograms of white granulated sugars and 0.2 kilogram of molecule distillating monoglyceride and 0.1 kilogram of octadecyl sodium lactate, thin up control material total amount is a double centner, cross 300 mesh sieve fine filterings then, get filtrate and be heated to 70 ℃, homogeneous twice under the condition of 25MPa, again 140 ℃ of 5 seconds of ultra high temperature sterilization, pour into then in the heat-resisting PET bottle, seal.Again through 121 ℃ of temperature sterilizations 30 minutes, what obtain is finished product.Contain 0.95% protein, 34% solubility in acid peptide yield, 0.9% fat, 6.72% soluble solid in this finished product, remaining amount is a water.Example 6
Remove the pinenut that 4.0 kilograms of shell pinenuts (protein 〉=15%) remove underwear and go rotten and go bad, put into 0.2% sodium hydroxide solution then, soaked 4 minutes in 80 ℃ of temperature, alkali lye was not advisable to have material, rinsed well with running water.Pinenut is levigate with three roller stone mills, the weight that added pinenut and water then and be 1: 15 is passed through colloid mill again, and mistake 100 mesh sieves, get the pinenut extract, to wherein adding 5 kilograms of As1398 neutral proteinases, PH is 8,50 ℃ of temperature, enzymolysis 1.5 hours, 80 ℃ of enzymes 15 minutes of going out add 6.5 kilograms of white granulated sugars, 0.3 kilogram of molecule distillating monoglyceride and 0.2 kilogram of octadecyl sodium lactate in the said goods then, thin up control material total amount is a double centner, cross 300 mesh sieve fine filterings then, get filtrate and be heated to 65 ℃ of homogeneous twice under 30MPa, again through 140 ℃ of 3 seconds of UHTS, outlet temperature is 90 ℃, enters sterile filling and is finished product.This finished product contains 0.6% protein, 28.10 solubility in acid peptide yield, 2.4% fat, 7.5% soluble solid, and remainder is a water.
Annotate: percentage used in the present patent application file all is weight percentage.Preparation main technique flow process of the present invention is as follows:
Fig. 1
Claims (6)
1, a kind of Pinenut polypeptide beverage is characterized in that: contain following composition in the drink:
Solubility in acid peptide yield 〉=25%
Protein 〉=0.5%
Fat 〉=0.6%
Soluble solid 〉=6%
The remaining amount of water
2, a kind of preparation method of Pinenut polypeptide beverage is characterized in that: a, raw material and handle 1. the raw material consumption and adopt following formula to calculate
2. preliminary treatment: soaked 4 minutes when temperature is 80 ℃ with will shell the pinenut of underwear of 0.2% sodium hydroxide solution, clean up then.3. defibrination: pinenut and water are mixed defibrination, and the weight ratio of pinenut and water is 1: 15b, enzymolysis 1. adopt protease hydrolyzed 2. the consumption of enzyme calculate with following formula
Wherein: W
Enzyme---the consumption of enzyme
W
Former---the material protein quality is enzymatic hydrolysis condition 3..30～50 ℃ of 1～2 hour time of temperature pH value is 7.5～8 enzymes that 4. go out: at 80～90 ℃ of temperature, 10～15 minutes c of enzyme that go out, allotment is allotment 1.: add white granulated sugar and 0.1～0.3% molecule distillating monoglyceride emulsifying agent and 0.1～0.3% octadecyl sodium lactate emulsifying agent with respect to finished weight 4～6% in enzymolysis product, 2. dilute filtration: to above-mentioned material thin up to normal concentration, cross 300 mesh sieves then, 3. homogeneous: the material dilution after will filtering is heated to 62～80 ℃ of temperature, twice of 24.5～30MPa homogeneous, 4. sterilization can: the spy that homogeneous is crossed expects that dilution is through 137～140 ℃ of ultra high temperature sterilizations 3～8 seconds, temperature 80～90 ℃ of cans of cooling rapidly then.
3, the preparation method of Pinenut polypeptide beverage according to claim 2 is characterized in that: raw material is pinenut and caseic mixture, and its pinenut consumption that shells is 1～2% finished weight, casein consumption 〉=0.4% finished weight.
4, the preparation method of Pinenut polypeptide beverage according to claim 2 is characterized in that: raw material is the mixture of pinenut and collagen, and its pinenut consumption that shells is 1～2% finished weight, collagen consumption 〉=0.4% finished weight.
5, the preparation method of Pinenut polypeptide beverage according to claim 2 is characterized in that: raw material is the mixture of pinenut and soybean, and its pinenut consumption that shells is 1～2% finished weight, soybean consumption 〉=1.5～2% finished weights.
6, the preparation method of Pinenut polypeptide beverage according to claim 5, it is characterized in that: the extracting method of soybean protein mixed liquor is, place 95 ℃ of water enzyme that carries out going out in 5～20 minutes to handle soybean, skin of beancurd is taken off, place the water of 3 times of soybean weight to soak abundant hydration, add 6 times of water mill slurries of soybean weight again and cross 150 eye mesh screens, adopt the deodorization of 19.6～26.7KPa vacuum.
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| CNB011389338A CN1313032C (en) | 2001-12-24 | 2001-12-24 | Pinenut polypeptide beverage and its preparation method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011389338A CN1313032C (en) | 2001-12-24 | 2001-12-24 | Pinenut polypeptide beverage and its preparation method |
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| CN1428106A true CN1428106A (en) | 2003-07-09 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102551142A (en) * | 2012-03-06 | 2012-07-11 | 椰树集团海南椰汁饮料有限公司 | Beverage and preparation method thereof |
| CN107397105A (en) * | 2017-07-24 | 2017-11-28 | 哈尔滨工业大学 | A kind of pine kernel protein peptide solid beverage rich in anthocyanidin suitable for gout patients |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1112135C (en) * | 2000-06-16 | 2003-06-25 | 北京市营养源研究所 | Easily soluble pine seed powder as solid beverage and its preparing process |
-
2001
- 2001-12-24 CN CNB011389338A patent/CN1313032C/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102551142A (en) * | 2012-03-06 | 2012-07-11 | 椰树集团海南椰汁饮料有限公司 | Beverage and preparation method thereof |
| CN102551142B (en) * | 2012-03-06 | 2013-05-15 | 椰树集团海南椰汁饮料有限公司 | Beverage and preparation method thereof |
| CN107397105A (en) * | 2017-07-24 | 2017-11-28 | 哈尔滨工业大学 | A kind of pine kernel protein peptide solid beverage rich in anthocyanidin suitable for gout patients |
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