CN1425918A - Quick test paper strip diagnostic reagent kits for bilharziasis and its preparing method and use - Google Patents
Quick test paper strip diagnostic reagent kits for bilharziasis and its preparing method and use Download PDFInfo
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- CN1425918A CN1425918A CN 03112697 CN03112697A CN1425918A CN 1425918 A CN1425918 A CN 1425918A CN 03112697 CN03112697 CN 03112697 CN 03112697 A CN03112697 A CN 03112697A CN 1425918 A CN1425918 A CN 1425918A
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Abstract
The present invention belongs to immunological parasitic disease diagnosis technology. The reagent kit is prepared via dye labeling process and includes labeling antigen solution compounded with 2BLN disperse blue dye, soluble schistosoma japoncium egg antigen, 1% sodium azide and bow serum albumen blocking liquid; and test paper strip with nitrocellulose as chromatographic film detection lines of human sheep antigen IgG and contrast lines of soluble schistosoma japonium egg resisting rabbit antigen IgG on the chromatographic film. The serum to be tested is mixed with the labeling antigen solution and the mixture liquid is tested with the test paper strip.
Description
Technical field
Quick test strips diagnostic kit of snail fever and its production and application adopts industrial dye to replace the mark so-called dye marker method of thing of enzyme, is used for the quick test strips diagnosis of snail fever, belongs to parasitic disease immune diagnostic technique field.
Background technology
At present, close with the present invention snail fever test strips diagnostic method has following two kinds:
1. Collaurum marking: the method for tetra chlorauric acid by reduction (mainly containing white phosphorus reduction, sodium borohydride reduction and tannic acid-citric acid three sodium reductions etc.) is mixed with diameter is about 5-10nm colloid gold particle solution, it is a kind of red solution, protein has positive charge because the collaurum surface has negative charge, both inhale and strong bonded mutually owing to static, so collaurum can be used as the label of protein.Its shortcoming is: (1) collaurum price is more expensive, and particularly finished product collaurum price is especially expensive, often is imported product, though the self-control collaurum can reduce cost, is difficult to obtain comparatively satisfied colloid gold particle; (2) the colloid gold label process is comparatively loaded down with trivial details, and condition is difficult for grasping, and needs can use behind the purifying; (3) the colloid gold label thing need be stored in 4 ℃, is unfavorable for transporting carrying.
2. enzyme labeling method: horseradish peroxidase combines with antibody protein by glutaraldehyde glue connection or sodium periodate method for oxidation.Its shortcoming is: the price of (1) enzyme is more expensive; (2) labeling method comparatively bothers, and needs can use behind the purifying; (3) the easy inactivation of enzyme need be stored in 4 ℃, is unfavorable for transporting carrying; Spended time is longer when (4) detecting, and generally about 2 hours, is unsuitable for on-the-spot extensive generaI investigation and uses.
Summary of the invention
The purpose of this invention is to provide quick test strips diagnostic kit of a kind of snail fever and its production and application, develop a kind of novelty, easy snail fever immune diagnostic technique.
Technical scheme
The quick test strips diagnostic kit of snail fever is by test strips (1), dye marker antigen liquid (2), target cuvette (3), target carriage (4) and box body (5) are formed, test strips has been sealed in to be covered in the plastic bottle, test strips PVC liner, the middle cellulose nitrate chromatographic film of pasting, adsorptive pads is pasted in the upper end, sample pad is pasted in the bottom, adsorptive pads and sample pad must have overlapping 0.1cm with chromatographic film, drawing detection line on the chromatographic film at 1cm place, distance bottom with the goat anti-human igg, on the chromatographic film at 2cm place, distance bottom, draw control line with rabbit anti schistosoma soluble egg antigen (SEA) IgG, the dye marker antigen liquid is sealed in the plastic tube, the dye marker antigen liquid is by Schistosoma japonicum soluble egg antigen (SEA), dyestuff stock solution and phosphate buffer (PBS) and Sodium azide PBS and bovine serum albumin(BSA) (BSA) confining liquid are formulated, dyestuff adopts 2BLN to disperse blue dyestuff, the target cuvette places in the target carriage during test, and the plastic bottle that test strips (1) is housed all is placed in the box body (5) with plastic tube that dye marker antigen liquid (2) is housed and the target carriage (4) that target cuvette (3) is housed.
The preparation method of mentioned reagent box is:
One, material
Dyestuff: 2BLN disperses blue dyestuff, is a kind of industrial homemade dyestuff, molecular formula: C
14H
9N
2O
4Br, molecular weight: 349.
Cellulose nitrate (NC) chromatographic film: U.S. Millipore company produces, and specification: Hi-Flow, permeability test are 120 ± 35 seconds.
Adsorptive pads: U.S. Millipore company produces, specification: AP22.
Sample pad: U.S. Millipore company produces, specification: AP22.
The PVC liner.
Target cuvette: diameter 0.6cm, high 1cm polyvinyl chloride plastic material products.
Sodium hydrogen phosphate (Na
2HPO
412H
2O MW=358.14).
Sodium dihydrogen phosphate (NaH
2PO
42H
2O MW=156.01).
Sodium chloride (NaCl MW=58.44).
Sodium azide (NaN
3MW=65.02).
Trehalose (C
12H
22O
112H
2O MW=378.33).
Bovine serum albumin(BSA) (BSA).
Phosphate buffer (PBS).
Schistosoma japonicum soluble egg antigen (SEA).
The goat anti-human igg.
Rabbit anti schistosoma soluble egg antigen (SEA) IgG.
Two, equipment
Supercentrifuge (20000 * g)
Spectrophotometer
Swing shaking table
Incubator
Three, reagent preparation
1.0.01M?PBS(pH7.4)
Na
2HPO
4·12H
2O 2.9g
NaH
2PO
4·2H
2O 0.3g
NaCl 8.0g
Adding distil water is to 1000ml
2. confining liquid
BSA 1.0g
Add 0.01M PBS (pH7.4) to 100ml
3.1% (W/V) Sodium azide PBS
Sodium azide 1.0g
Add 0.01M PBS (pH7.4) to 100ml
4.SEA preparation
With the schistosoma japonicum cercariae infected rabbits, get the rabbit liver after 45 days and separate worm's ovum, add physiological saline and put and grind 1h on the ice bath, centrifugal 14000rpm, 1h collects supernatant and is SEA.
5. goat anti-human igg
Affinity chromatography product (available from the magnificent company in Shanghai) is diluted to 1mg/ml with PBS during use, and with trehalose, making the trehalose final concentration is 0.15M, is abbreviated as to contain the 0.15M trehalose.
6. the preparation of rabbit anti schistosoma soluble egg antigen (SEA) IgG
With the outer immunization immunizing rabbit of standard body, make antigen with SEA, each 2mg adds the Fu Shi Freund's complete adjuvant, adopts the immunity of intracutaneous multi-point injection method, immunity is 3 times altogether, gather rabbit blood when above when the immunize rabbit serum titer reaches 1: 10240, separation of serum is collected IgG with Protein A affinity chromatography purifying again, be diluted to 1mg/ml with PBS during use, contain the 0.15M trehalose.
Four, method
1. dyestuff stock solution preparation:
Dyestuff 0.5g
Distilled water 10ml
Centrifugal 15000 * g behind the abundant suspendible, 30min abandons supernatant, precipitation adding distil water 10ml, centrifugal 15000 * g behind the abundant suspendible, 30min repeats 4 times (washing altogether 5 times), after finishing, abandon supernatant, precipitation adding distil water 10ml, fully centrifugal 250 * g behind the suspendible, 30min gets supernatant as the dyestuff stock solution.
Measure the A value of dyestuff stock solution: the dyestuff stock solution that takes a morsel transfers to 630nm with 100 times of distilled water dilutings with the spectrophotometer wavelength, with the distilled water zeroing, reads extinction (OD) value, A=OD
630nmValue * 100.
Add 1% Sodium azide PBS in the dyestuff stock solution, making the Sodium azide final concentration is 0.05%, is stored under the room temperature standby.
2. dye marker antigen liquid preparation
SEA 1mg
Dyestuff stock solution xml (x=60/ dyestuff stock solution A value)
PBS (0.01M pH7.4) adds to 5ml
Above-mentioned material is put in the 10ml centrifuge tube, put into 37 ℃ of incubators and shake the 2h taking-up back and forth, add confining liquid 5ml, continue in 37 ℃ of incubators and shake 4h back and forth, take out centrifugal 15000 * g, 30min, 4 ℃, abandon supernatant, precipitation adds the 10ml confining liquid, centrifugal 15000 * g behind the mixing, 30min, 4 ℃, abandon supernatant, precipitation adds confining liquid 5ml, will precipitate piping and druming gently evenly with suction pipe, must beat to there not being macroscopic coarse particle, add 1% Sodium azide PBS, making the Sodium azide final concentration is 0.05%, and this is the dye marker stock solution, dilute 8 times with confining liquid during use, replenish and to add 1% nitrine and receive PBS, making nitrine receive final concentration is 0.05%, (be abbreviated as contain 0.05% nitrine receive), be the dye marker antigen liquid, stock solution and antigen liquid all can be in room temperature preservation.
3. test strips preparation
It is long that the PVC liner is cut into 5cm, sticks 2.5cm cellulose nitrate chromatographic film in the centre, sticks the long adsorptive pads of 2.25cm in the upper end, sticks the long sample pad of 0.5cm in the bottom, adsorptive pads and sample pad must with the two ends overlapping 0.1cm of cellulose nitrate chromatographic film.On the chromatographic film at 1cm place, distance bottom, (be diluted to 1mg/ml with PBS with the goat anti-human igg, contain the 0.15M trehalose) draw and go up the wide detection line of 0.05cm, on the chromatographic film at 2cm place, distance bottom, (be diluted to 1mg/ml with PBS with rabbit anti schistosoma soluble egg antigen (SEA) IgG, contain the 0.15M trehalose) draw and go up the wide control line of 0.05cm, put 37 ℃ 2 hours, the film that processes is cut into the wide bar of 0.3cm is test strips.
The detection of mentioned reagent box is used: get the target cuvette, add 50 μ l dye marker antigen liquids and 10 μ l serum to be checked, fully mixing 1min inserts test strips then, and sample pad one end is inserted the cup end, observations behind the 5-10min.The result judges: the positive reaction of purple blue all appears in detection line and control line, and purple blue appears in control line and detection line does not have the negative reaction of purple blue.
Points for attention:
1. detecting serum must be fresh, otherwise can influence testing result.
2. when getting test strips, pinch adsorptive pads (long end), forbid to touch the detection film with hand.
3. at the bottom of test strips must be inserted glass.
4. this test strips and lung fluke patient and liver fluke patient have the part cross reaction, note during detection.
Beneficial effect
1. mark is a kind of industrial homemade dyestuff with the 2BLN dyestuff, be used for immunodiagnosis and be initiative, and price is very cheap.
2. labeling method is easy.
3. test strips that mark is good and antigen liquid steady quality can transport at room temperature, and can preserve more than 1 year at room temperature.This technology also is proprietary.
4. method of testing is easy, need not any instrument and equipment.General personnel can operate according to explanation, do not need special training.
5. method of testing is quick, and whole process only need can be finished detection in about 10 minutes.
Description of drawings
Fig. 1 kit composition diagram.1, test strips; 2, labeling dye antigen liquid; 3, target cuvette; 4, target carriage; 5, box body.
Fig. 2 is a test strips test design sketch: the A positive reaction: the purple blue band all appears in detection band and contrast band; The B negative reaction: contrast is taken existing purple blue band out of and is detected the no purple blue band of band.
The FB(flow block) of Fig. 3 preparation method and application.
Specific implementation method
Embodiment 1: the method for recording and narrating in the by specification, the composition of prepared kit comprise 48 test strips, 3ml dye marker antigen liquid, 48 PVC target cuvettes.
Embodiment 2: detect serum of acute schistosomicide patient 30 examples, chronic schistosomiasis human serum 84 examples with the quick test strips diagnostic kit of snail fever, its susceptibility is respectively 96.7% and 94.0%, detects healthy human serum 60 examples, and its specificity is 96.7%.Joden ' s index is respectively 0.967+0.967-1=0.934; 0.94+0.967-1=0.907.Show the susceptibility height of this method, specificity is good.Be a kind of immunodiagnosis kit of snail fever efficiently.
---select from " Chinese prevention and cure of snail fever magazine " and rolled up the 18th page to 30 pages of the 1st phases in 2000 the 12nd
Embodiment 3: rig-site utilization: calendar year 2001 is detected positive snail fever human serum 121 examples of Jiangxi Province's BoYang County excrement inspection with the quick test strips diagnostic kit of snail fever, positive rate is 96.69% (117/121), 30 routine health are negative per capita, specificity is 100%, and Joden ' s index can reach 0.967+1.000-1=0.967.Show the susceptibility height of this method, specificity is good, and disease is looked at the scene of being applicable to.
---select from the 28th page to 30 pages of the 14th the 1st phases of volume of " Chinese preventing and treating verminosis magazine " calendar year 2001
Embodiment 4: rig-site utilization: calendar year 2001 adopts the quick test strips diagnostic kit of snail fever to detect Jiangxi Province's Dexing City (snail fever has been eliminated 15 years) 20000 routine crowd's serum, negative match-rate reaches 99.3% (specificity), and the positive coincidence rate of 30 routine excrement inspection positive serums of contrast is 93.3% (28/30), the healthy philtrum in the 30 non-popular districts of example has 1 example positive, and specificity is 96.7% (1/30).Show the susceptibility height of this method, specificity is good.
---select from the 304th page of the 13rd the 5th phase of volume of " Chinese prevention and cure of snail fever magazine " calendar year 2001
Embodiment 5: rig-site utilization: 2002 in suburb, Nanjing, Jiangsu Province (snail fever low popular district) use the quick test strips diagnostic kit of snail fever and carry out snail fever and eliminate examination up to standard, look into 2788 people altogether, positive rate only is 0.29%.Conform to the popular situation in locality, and expense saves 23.76% than other immune diagnostic methods.So this method also is applicable to snail fever examination up to standard.
---select from " Chinese prevention and cure of snail fever magazine " and rolled up the 374th page to 375 pages of the 5th phases in 2002 the 14th
Embodiment 6: rig-site utilization: 2002 in Dantu county, Zhengjiang City, Jiangsu Province (snail fever low popular district) with snail fever in the examination of quick test strips diagnostic kit to 463 people (natural crowd), the 18 positive blood fluke patients of routine excrement inspection, it is positive to detect 17 examples, and positive coincidence rate is 94.4%.The positive coincidence rate of the ring ovum precipitation method (COPT) then is 72.2%.Show that this method is applicable to the low popular district examination of snail fever, more suitable than other immune diagnostic methods.
---select from " Chinese prevention and cure of snail fever magazine " 2003 the 15th the 1st phases of volume
Embodiment 7: rig-site utilization: 2002 suburb, Nanjing, Jiangsu Province (snail fever low popular district) with snail fever quick test strips diagnostic kit to 465 routine natural crowd's examinations in, the 13 positive blood fluke patients of routine excrement inspection, the result is positive entirely, and positive coincidence rate is 100%.Show that this method is applicable to that the low popular district examination of snail fever determines the chemotherapy object.
---article is waited to deliver
Claims (9)
1. quick test strips diagnostic kit of snail fever, it is characterized in that by test strips (1), dye marker antigen liquid (2), target cuvette (3), target carriage (4) and box body (5) are formed, test strips has been sealed in to be covered in the plastic bottle, test strips PVC liner, the middle cellulose nitrate chromatographic film of pasting, adsorptive pads is pasted in the upper end, sample pad is pasted in the bottom, adsorptive pads and sample pad must have overlapping 0.1cm with chromatographic film, drawing detection line with goat-anti people LgG on the chromatographic film at 1cm place, distance bottom, on the chromatographic film at 2cm place, distance bottom, draw control line with rabbit anti schistosoma soluble egg antigen (SEA) LgG, the dye marker antigen liquid is sealed in the plastic tube, the dye marker antigen liquid is by Schistosoma japonicum soluble egg antigen (SEA), dyestuff stock solution and phosphate buffer (PBS) and Sodium azide PBS and bovine serum albumin(BSA) (BSA) confining liquid are formulated, dyestuff adopts 2BLN to disperse blue dyestuff, the target cuvette places in the target carriage during test, and the plastic bottle that test strips (1) is housed all is placed in the box body (5) with plastic tube that dye marker antigen liquid (2) is housed and the target carriage (4) that target cuvette (3) is housed.
2. kit according to claim 1 is characterized in that the composition of kit comprises 48 test strips, 3ml dye marker antigen liquid, 48 PVC target cuvettes.
3. method of making the kit of claim 1 is characterized in that:
Preparation dye marker antigen liquid
(1) preparation dyestuff stock solution
Get 0.5g 2BLN and disperse blue dyestuff, adding distil water 10ml washing, centrifugal 15000 * g behind the abundant suspendible, 30min abandons supernatant, repeated washing 4 times is washed 5 times altogether, washes completely, abandons supernatant, precipitation adding distil water 10ml, centrifugal 250 * g behind the abundant suspendible, 30min gets supernatant as the dyestuff stock solution;
Add 1% Sodium azide PBS in the dyestuff stock solution, making the Sodium azide final concentration is 0.05%, is stored under the room temperature standby;
Measure the A value of dyestuff stock solution: the dyestuff stock solution that takes a morsel transfers to 630nm with 100 times of distilled water dilutings with the spectrophotometer wavelength, with the distilled water zeroing, reads extinction (OD) value, A=OD
630nmValue * 100;
(2) preparation dye marker antigen liquid
Get antigen SEA 1mg, dyestuff stock solution xml (x=60/ dyestuff stock solution A value) adds PBS (0.01M pH7.4) to 5ml, above-mentioned material is put in the 10ml centrifuge tube, put into 37 ℃ of incubators and shake the 2h taking-up back and forth, add confining liquid 5ml, continue in 37 ℃ of incubators and shake 4h back and forth, take out centrifugal 15000 * g, 30min, abandons supernatant by 4 ℃, precipitation adds the 10ml confining liquid, centrifugal 15000 * g behind the mixing, 30min, 4 ℃, abandon supernatant, precipitation adds confining liquid 5ml, will precipitate piping and druming gently evenly with suction pipe, must beat to there not being macroscopic coarse particle, add 1% Sodium azide PBS, making the Sodium azide final concentration is 0.05%, and this is the dye marker stock solution, dilutes 8 times (containing 0.05% Sodium azide) with confining liquid during use, be the dye marker antigen liquid, stock solution and antigen liquid all can be in room temperature preservation;
The test strips preparation:
The PVC liner is cut into 5cm length, stick 2.5cm cellulose nitrate chromatographic film in the centre, stick the long adsorptive pads of 2.25cm in the upper end, stick the long sample pad of 0.5cm in the bottom, adsorptive pads and sample pad must with the two ends overlapping 0.1cm of cellulose nitrate chromatographic film, on the chromatographic film at 1cm place, distance bottom, (be diluted to 1mg/ml with PBS with the goat anti-human igg, contain the 0.15M trehalose) draw and go up the wide detection line of 0.05cm, on the chromatographic film at 2cm place, distance bottom, (be diluted to 1mg/ml with PBS with rabbit anti schistosoma soluble egg antigen (SEA) IgG, contain the 0.15M trehalose) draw and go up the wide control line of 0.05cm, put 37 ℃ 2 hours, the film that processes is cut into the wide bar of 0.3cm is test strips.
4. the preparation method of kit according to claim 2 is characterized in that the preparation of confining liquid: get bovine serum albumin(BSA) (BSA) 1g, add 0.01M PBS (pH7.4) to 100ml.
5. the preparation method of kit according to claim 2 is characterized in that the preparation of 1% Sodium azide PBS: get Sodium azide 1g, add 0.01M PBS (pH7.4) to 100ml.
6. the preparation method of kit according to claim 2, the preparation that it is characterized in that Schistosoma japonicum soluble egg antigen (SEA) is with the schistosoma japonicum cercariae infected rabbits, get the rabbit liver after 45 days and separate worm's ovum, adding physiological saline puts and grinds 1h on the ice bath, centrifugal 14000rpm, 1h collects supernatant and is SEA.
7. the preparation method of kit according to claim 2 is characterized in that goat-anti people 1gG, is diluted to 1mg/ml with PBS during use, contains the 0.15M trehalose.
8. the preparation method of kit according to claim 2, it is characterized in that the preparation of rabbit anti schistosoma soluble egg antigen (SEA) 1gG: with the outer immunization immunizing rabbit of standard body, make antigen with SEA, each 2mg, add the Fu Shi Freund's complete adjuvant, adopt the immunity of intracutaneous multi-point injection method, immunity is 3 times altogether, when reaching 1: 10240, the immunize rabbit serum titer gathers rabbit blood when above, separation of serum, collect IgG with Protein A affinity chromatography purifying again, be diluted to 1mg/ml with PBS during use, contain the 0.15M trehalose.
9. the detection of kit according to claim 1 is used, it is characterized in that getting the target cuvette, add 50 μ l dye marker antigen liquids and 10 μ l serum to be checked, abundant mixing 1min, insert test strips then, sample pad one end is inserted the cup end, observations behind the 5-10min, the result judges: the positive reaction of purple blue all appears in detection line and control line, and purple blue appears in control line and detection line does not have the negative reaction of purple blue.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031126979A CN1184481C (en) | 2003-01-14 | 2003-01-14 | Quick test paper strip diagnostic reagent kits for bilharziasis and its preparing method and use |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB031126979A CN1184481C (en) | 2003-01-14 | 2003-01-14 | Quick test paper strip diagnostic reagent kits for bilharziasis and its preparing method and use |
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|---|---|
| CN1425918A true CN1425918A (en) | 2003-06-25 |
| CN1184481C CN1184481C (en) | 2005-01-12 |
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|---|---|---|---|
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100383162C (en) * | 2005-05-10 | 2008-04-23 | 安徽医科大学 | Combination detection reagent for detecting schistosomiasis and detecting method thereof |
| CN1690711B (en) * | 2004-04-23 | 2010-04-14 | 中国人民解放军军事医学科学院微生物流行病研究所 | Immune chromatographic test paper bar based on up conversion luminescence technology |
| CN101038289B (en) * | 2006-03-15 | 2012-05-02 | 中国农业科学院上海家畜寄生虫病研究所 | Test paper for rapidly diagnosing livestocks schistosomiasis japonica |
| CN102520149A (en) * | 2011-12-26 | 2012-06-27 | 江苏省血吸虫病防治研究所 | Method for detecting medicine resistance of schistosoma japonicum cercariae to praziquantel in vitro |
| CN103076447A (en) * | 2012-12-27 | 2013-05-01 | 上海市疾病预防控制中心 | Schistosoma egg crude antigen purification method, related purified antigen and schistosoma antibody detection colloidal gold immunoassay kit |
| CN104101709A (en) * | 2013-04-12 | 2014-10-15 | 北京普析通用仪器有限责任公司 | Enzyme linked immunosorbent assay kit and detection method of rhodamine B |
| CN105254732A (en) * | 2015-11-23 | 2016-01-20 | 中国医学科学院病原生物学研究所 | Schistosoma japonicum katsurada recombinant protein SjSAPLP5 as well as encoding gene and application thereof |
| CN105384803A (en) * | 2015-11-23 | 2016-03-09 | 中国医学科学院病原生物学研究所 | Schistosoma japonicum katsurada recombinant protein SjSAPLP4 as well as encoding gene and application thereof |
| CN109870580A (en) * | 2017-12-01 | 2019-06-11 | 江苏省血吸虫病防治研究所 | Serum protein markers group identifies application and detection kit in schistosomiasis detection reagent box in preparation |
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2003
- 2003-01-14 CN CNB031126979A patent/CN1184481C/en not_active Expired - Fee Related
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1690711B (en) * | 2004-04-23 | 2010-04-14 | 中国人民解放军军事医学科学院微生物流行病研究所 | Immune chromatographic test paper bar based on up conversion luminescence technology |
| CN100383162C (en) * | 2005-05-10 | 2008-04-23 | 安徽医科大学 | Combination detection reagent for detecting schistosomiasis and detecting method thereof |
| CN101038289B (en) * | 2006-03-15 | 2012-05-02 | 中国农业科学院上海家畜寄生虫病研究所 | Test paper for rapidly diagnosing livestocks schistosomiasis japonica |
| CN102520149A (en) * | 2011-12-26 | 2012-06-27 | 江苏省血吸虫病防治研究所 | Method for detecting medicine resistance of schistosoma japonicum cercariae to praziquantel in vitro |
| CN103076447B (en) * | 2012-12-27 | 2015-04-08 | 上海市疾病预防控制中心 | Schistosoma egg crude antigen purification method, related purified antigen and schistosoma antibody detection colloidal gold immunoassay kit |
| CN103076447A (en) * | 2012-12-27 | 2013-05-01 | 上海市疾病预防控制中心 | Schistosoma egg crude antigen purification method, related purified antigen and schistosoma antibody detection colloidal gold immunoassay kit |
| CN104101709A (en) * | 2013-04-12 | 2014-10-15 | 北京普析通用仪器有限责任公司 | Enzyme linked immunosorbent assay kit and detection method of rhodamine B |
| CN104101709B (en) * | 2013-04-12 | 2016-08-31 | 北京普析通用仪器有限责任公司 | A kind of enzyme linked immunological kit detecting rhodamine B and detection method |
| CN105254732A (en) * | 2015-11-23 | 2016-01-20 | 中国医学科学院病原生物学研究所 | Schistosoma japonicum katsurada recombinant protein SjSAPLP5 as well as encoding gene and application thereof |
| CN105384803A (en) * | 2015-11-23 | 2016-03-09 | 中国医学科学院病原生物学研究所 | Schistosoma japonicum katsurada recombinant protein SjSAPLP4 as well as encoding gene and application thereof |
| CN105254732B (en) * | 2015-11-23 | 2018-08-28 | 中国医学科学院病原生物学研究所 | A kind of Schistosoma japonicum recombinant protein SjSAPLP5 and its encoding gene and application |
| CN105384803B (en) * | 2015-11-23 | 2018-08-31 | 中国医学科学院病原生物学研究所 | A kind of Schistosoma japonicum recombinant protein SjSAPLP4 and its encoding gene and application |
| CN109870580A (en) * | 2017-12-01 | 2019-06-11 | 江苏省血吸虫病防治研究所 | Serum protein markers group identifies application and detection kit in schistosomiasis detection reagent box in preparation |
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| CN1184481C (en) | 2005-01-12 |
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