CN102520149A - Method for detecting medicine resistance of schistosoma japonicum cercariae to praziquantel in vitro - Google Patents
Method for detecting medicine resistance of schistosoma japonicum cercariae to praziquantel in vitro Download PDFInfo
- Publication number
- CN102520149A CN102520149A CN2011104413353A CN201110441335A CN102520149A CN 102520149 A CN102520149 A CN 102520149A CN 2011104413353 A CN2011104413353 A CN 2011104413353A CN 201110441335 A CN201110441335 A CN 201110441335A CN 102520149 A CN102520149 A CN 102520149A
- Authority
- CN
- China
- Prior art keywords
- praziquantel
- schistosoma japonicum
- mol
- cercaria
- docking
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention relates to a method for detecting the medicine resistance of schistosoma japonicum cercariae to praziquantel in vitro, and belongs to the technical field of antiparasitic medicines. The method comprises the following steps of: adding 1mL of 10<-5> mol/L praziquantel liquor, 1mL of 5*10<-6> mol/L praziquantel liquor, 1mL of 10<-6> mol/L praziquantel liquor and 1mL of 5*10<-7> mol/L praziquantel liquor into plate holes of a 24-hole cell culture plate, immigrating 20 to 50 newly escaped schistosoma japonicum cercariae by using an inoculating loop, observing the docking change of the cercariae in the holes under an anatomical lens after the praziquantel liquor acts for 20 minutes, and calculating the docking rate of the cercariae, wherein the docking rate of the cercariae is less than 40 percent after 10<-5> mol/L praziquantel acts, is less than 35 percent after 5*10<-6> mol/L praziquantel acts, is less than 30 percent after 10<-6> mol/L praziquantel acts, and is less than 10 percent after 5*10<-7> mol/L praziquantel acts, so that the schistosoma japonicum cercariae have medicine resistance to the praziquantel. The method is quick, simple, convenient, sensitive and reliable and is suitable for quickly detecting and monitoring the medicine resistance of the schistosoma japonicum cercariae on site.
Description
Technical field
A kind of vitro detection schistosoma japonicum cercariae belongs to the anti-parasite medicine technical field to the drug-fast method of praziquantel.
Background technology
Japanese schistosomiasis is that a kind of harm humans is healthy, influences the serious infectious diseases of socio-economic development.Praziquantel is the current unique commercialization medicine that is used to treat Japanese schistosomiasis.Use repeatedly on a large scale with a kind of pest-resistant medicine for a long time and tend to cause the drug-fast generation of medicine, therefore be necessary to set up field monitoring and detect the drug-fast technology of praziquantel.The drug-fast main method of current detection praziquantel is the on-scene care patient in the epidemic-stricken area, assessment chemotherapy result, and this method time is long, patient's succession is low, and exists the under-enumeration leakage to control phenomenon.We have set up a kind of vitro detection schistosoma japonicum cercariae to the drug-fast technology of praziquantel in the laboratory; Can be easily and fast, the responsive resistance to the action of a drug that detects schistosoma japonicum cercariae to praziquantel; Solved the technical barrier that the praziquantel resistance to the action of a drug is difficult to detect, helped field monitoring and detect the drug-fast generation of Schistosoma japonicum praziquantel.
Summary of the invention
The purpose of this invention is to provide a kind of vitro detection schistosoma japonicum cercariae to the drug-fast method of praziquantel, solve field monitoring and detect the drug-fast technical matters of Schistosoma japonicum praziquantel, quick, easy, responsive, reliable.
Technical scheme of the present invention: a kind of vitro detection schistosoma japonicum cercariae is got one 24 porocyte culture plate to the drug-fast method of praziquantel, in plate hole, adds concentration and is respectively 10
-5, 5 * 10
-6, 10
-6With 5 * 10
-7Mol/L praziquantel soup 1 mL subsequently with 20 ~ 50 new schistosoma japonicum cercariaes of overflowing of oese immigration, under anatomical lens, observing cercaria docking variation in each hole behind praziquantel soup effect 20 min, calculates cercaria docking rate.Experiment repetition 6 times.Through observing cercaria docking rate, can detect the resistance to the action of a drug of schistosoma japonicum cercariae to praziquantel.
The cercaria docking changes, and is meant after the effect of praziquantel soup, and the body of schistosoma japonicum cercariae and portion are from breaking off.
The praziquantel soup is that praziquantel is dissolved in that to be mixed with concentration in 1% dimethyl sulfoxide (DMSO) (DMSO) be 1 * 10
-3Mol/L and water diluted for use.
A kind of vitro detection schistosoma japonicum cercariae adds concentration earlier and is respectively 10 the drug-fast method of praziquantel in 24 porocytes are cultivated plate hole
-5, 5 * 10
-6, 10
-6With 5 * 10
-7Each 1 mL of mol/L praziquantel soup moves into 20～50 new schistosoma japonicum cercariaes of overflowing with oese then, behind praziquantel soup effect 20 min, under anatomical lens, observes the cercaria docking and changes, and calculates cercaria docking rate.Experiment repetition 6 times.At warp 10
-5Behind mol/L praziquantel soup effect 20 min, cercaria docking rate<40%; Through 5 * 10
-6Behind mol/L praziquantel soup effect 20 min, cercaria docking rate<35%; Through 10
-6Behind mol/L praziquantel soup effect 20 min, cercaria docking rate<30%; Through 5 * 10
-7Behind mol/L praziquantel soup effect 20 min, cercaria docking rate<10%, can think that then this schistosoma japonicum cercariae has the resistance to the action of a drug to praziquantel.
Beneficial effect of the present invention: a kind of vitro detection schistosoma japonicum cercariae is to the drug-fast method of praziquantel; Fast, easy, responsive, reliable; Be fit to field quick detection and the drug-fast generation of monitoring Schistosoma japonicum praziquantel, can solve the technical matters of on-the-spot detection of the Schistosoma japonicum praziquantel resistance to the action of a drug and monitoring.
Embodiment
Embodiment 1: Schistosoma japonicum praziquantel sensitive strain and resistant strain cercaria are to the sensitivity testing of praziquantel
Get one 24 porocyte culture plate, in plate hole, adding concentration is 10
-5Praziquantel soup 1 mL of mol/L subsequently with the Schistosoma japonicum praziquantel sensitive strain and the resistant strain cercaria of 20 ~ 50 fresh effusions of oese immigration, observes cercaria docking variation in each hole under anatomical lens behind 20 min, calculate cercaria docking rate.Experiment repetition 6 times.
Table 1 praziquantel is to the influence of Schistosoma japonicum praziquantel sensitive strain and resistant strain cercaria docking variation
| The worm strain | Action time (min) | Praziquantel solution concentration (mol/L) | Cercaria docking rate (%) |
| Schistosoma japonicum praziquantel sensitive strain | 20 | 10 -5 | 68.7±8.9 |
| Schistosoma japonicum praziquantel resistant strain | 20 | 10 -5 | 36.2±6.4 |
Embodiment 2: Schistosoma japonicum praziquantel sensitive strain and resistant strain cercaria are to the sensitivity testing of praziquantel
Get one 24 porocyte culture plate, in plate hole, adding concentration is 5 * 10
-6Praziquantel soup 1 mL of mol/L subsequently with the Schistosoma japonicum praziquantel sensitive strain and the resistant strain cercaria of 20 ~ 50 fresh effusions of oese immigration, observes cercaria docking variation in each hole under anatomical lens behind 20 min, calculate cercaria docking rate.Experiment repetition 6 times.
Table 2 praziquantel is to the influence of Schistosoma japonicum praziquantel sensitive strain and resistant strain cercaria docking variation
| The worm strain | Action time (min) | Praziquantel solution concentration (mol/L) | Cercaria docking rate (%) |
| Schistosoma japonicum praziquantel sensitive strain | 20 | 5×10 -6 | 65.4±9.7 |
| Schistosoma japonicum praziquantel resistant strain | 20 | 5×10 -6 | 33.3±1.3 |
Embodiment 3: Schistosoma japonicum praziquantel sensitive strain and resistant strain cercaria are to the sensitivity testing of praziquantel
Get one 24 porocyte culture plate, in plate hole, adding concentration is 10
-6Praziquantel soup 1 mL of mol/L subsequently with the Schistosoma japonicum praziquantel sensitive strain and the resistant strain cercaria of 20 ~ 50 fresh effusions of oese immigration, observes cercaria docking variation in each hole under anatomical lens behind 20 min, calculate cercaria docking rate.Experiment repetition 6 times.
Table 3 praziquantel is to the influence of Schistosoma japonicum praziquantel sensitive strain and resistant strain cercaria docking variation
| The worm strain | Action time (min) | Praziquantel solution concentration (mol/L) | Cercaria docking rate (%) |
| Schistosoma japonicum praziquantel sensitive strain | 20 | 10 -6 | 43.8±7.0 |
| Schistosoma japonicum praziquantel resistant strain | 20 | 10 -6 | 28.6±1.5 |
Embodiment 4: Schistosoma japonicum praziquantel sensitive strain and resistant strain cercaria are to the sensitivity testing of praziquantel
Get one 24 porocyte culture plate, in plate hole, adding concentration is 5 * 10
-7Praziquantel soup 1 mL of mol/L subsequently with the Schistosoma japonicum praziquantel sensitive strain and the resistant strain cercaria of 20 ~ 50 fresh effusions of oese immigration, observes cercaria docking variation in each hole under anatomical lens behind 20 min, calculate cercaria docking rate.Experiment repetition 6 times.
Table 4 praziquantel is to the influence of Schistosoma japonicum praziquantel sensitive strain and resistant strain cercaria docking variation
| The worm strain | Action time (min) | Praziquantel solution concentration (mol/L) | Cercaria docking rate (%) |
| Schistosoma japonicum praziquantel sensitive strain | 20 | 5×10 -7 | 19.2±7.5 |
| Schistosoma japonicum praziquantel resistant strain | 20 | 5×10 -7 | 8.6±2.8 |
Claims (4)
1. a vitro detection schistosoma japonicum cercariae is characterized in that getting one 24 porocyte culture plate to the drug-fast method of praziquantel, in plate hole, adds concentration and is respectively 10
-5, 5 * 10
-6, 10
-6And 5 * 10
-7Mol/L praziquantel soup 1 mL; Move into 20 ~ 50 new schistosoma japonicum cercariaes of overflowing with oese subsequently; Under anatomical lens, observing cercaria docking variation in each hole behind praziquantel soup effect 20 min, calculate cercaria docking rate, experiment repetition 6 times; Through observing cercaria docking rate, detect the resistance to the action of a drug of schistosoma japonicum cercariae to praziquantel.
2. ask 1 described vitro detection schistosoma japonicum cercariae to the drug-fast method of praziquantel according to right power, it is characterized in that the cercaria docking changes, be meant that after the effect of praziquantel soup the body of schistosoma japonicum cercariae and portion are from breaking off.
3. ask 1 described vitro detection schistosoma japonicum cercariae to the drug-fast method of praziquantel according to right power, it is characterized in that the praziquantel soup is that praziquantel is dissolved in that to be mixed with concentration among the 1% dimethyl sulfoxide (DMSO) DMSO be 1 * 10
-3Mol/L and water diluted for use.
According to claim 1,2 or 3 described vitro detection schistosoma japonicum cercariaes to the drug-fast method of praziquantel, it is characterized in that in 24 porocytes are cultivated plate hole, adding earlier concentration is respectively 10
-5, 5 * 10
-6, 10
-6And 5 * 10
-7Each 1mL of mol/L praziquantel soup moves into 20～50 new schistosoma japonicum cercariaes of overflowing with oese then, and behind praziquantel soup effect 20 min, the cercaria docking changes in each hole of observation under anatomical lens, calculates cercaria docking rate, and experiment repetition 6 times is at warp 10
-5Behind mol/L praziquantel soup effect 20 min, cercaria docking rate<40%; Through 5 * 10
-6Behind mol/L praziquantel soup effect 20 min, cercaria docking rate<35%; Through 10
-6Behind mol/L praziquantel soup effect 20 min, cercaria docking rate<30%; Through 5 * 10
-7Behind mol/L praziquantel soup effect 20 min, cercaria docking rate<10%, think that promptly this schistosoma japonicum cercariae has the resistance to the action of a drug to praziquantel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104413353A CN102520149A (en) | 2011-12-26 | 2011-12-26 | Method for detecting medicine resistance of schistosoma japonicum cercariae to praziquantel in vitro |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104413353A CN102520149A (en) | 2011-12-26 | 2011-12-26 | Method for detecting medicine resistance of schistosoma japonicum cercariae to praziquantel in vitro |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102520149A true CN102520149A (en) | 2012-06-27 |
Family
ID=46291137
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011104413353A Pending CN102520149A (en) | 2011-12-26 | 2011-12-26 | Method for detecting medicine resistance of schistosoma japonicum cercariae to praziquantel in vitro |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102520149A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1425918A (en) * | 2003-01-14 | 2003-06-25 | 江苏省血吸虫病防治研究所 | Quick test paper strip diagnostic reagent kits for bilharziasis and its preparing method and use |
| CN101002553A (en) * | 2006-01-20 | 2007-07-25 | 赣南师范学院 | High efficiency agent for preventing and controlling shistosomulum, and preparing technique therefor |
| WO2010107791A2 (en) * | 2009-03-17 | 2010-09-23 | Concert Pharmaceuticals, Inc. | Pyrazinoisoquinoline compounds |
-
2011
- 2011-12-26 CN CN2011104413353A patent/CN102520149A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1425918A (en) * | 2003-01-14 | 2003-06-25 | 江苏省血吸虫病防治研究所 | Quick test paper strip diagnostic reagent kits for bilharziasis and its preparing method and use |
| CN101002553A (en) * | 2006-01-20 | 2007-07-25 | 赣南师范学院 | High efficiency agent for preventing and controlling shistosomulum, and preparing technique therefor |
| WO2010107791A2 (en) * | 2009-03-17 | 2010-09-23 | Concert Pharmaceuticals, Inc. | Pyrazinoisoquinoline compounds |
Non-Patent Citations (4)
| Title |
|---|
| 乐文菊，等: "日本血吸虫对吡喹酮抗药性的研究", 《中国血吸虫病防治杂志》 * |
| 李洪军，等: "血吸虫对吡喹酮抗药性的研究XIV日本血吸虫吡喹酮抗性株和敏感株成虫毛蚴和尾蚴对吡喹酮敏感性比较", 《中国血吸虫病防治杂志》 * |
| 杭盘宇，等: "不同阶段日本血吸虫对吡喹酮敏感性的研究", 《中国血吸虫病防治杂志》 * |
| 梁幼生，等: "血吸虫对吡喹酮抗药性的研究Ⅺ日本血吸虫中国大陆现场分离株虫卵毛蚴尾蚴对吡喹酮的敏感性", 《中国血吸虫病防治杂志》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US10383555B2 (en) | Detection of contamination at sensor contacts | |
| MX336368B (en) | Method for monitoring a sterilization process. | |
| BRPI0718525A8 (en) | SYSTEM TO GENERATE OUTPUT SIGNALS INDICATIVE OF THE QUALITY OF CONTACT OF SEVERAL ELECTRODES, AND METHOD TO DETERMINE QUALITY OF CONTACT OF SEVERAL ELECTRODES | |
| NZ627443A (en) | Biological markers for identifying patients for treatment with vegf antagonists | |
| GB2566217A (en) | A toxicant monitoring system | |
| BR112014008842A2 (en) | system for detecting and monitoring bodily waste by gas-emitting substance for use in interactive toilet training | |
| Dong et al. | Involvement of autophagy induction in penta-1, 2, 3, 4, 6-O-galloyl-β-D-glucose-induced senescence-like growth arrest in human cancer cells | |
| AR086536A1 (en) | METHOD FOR THE RESELECTION OF CELLS AND THE TRANSFER BETWEEN CELLS IN A WIRELESS COMMUNICATION SYSTEM | |
| BR112014001423A2 (en) | test to capture and detect circulating multiple myeloma cells in the blood | |
| GB2532559A (en) | Detection of volatiles in personal care products | |
| CN102520149A (en) | Method for detecting medicine resistance of schistosoma japonicum cercariae to praziquantel in vitro | |
| CN103720685A (en) | Application of quercetin in preparation of medicines for treating mitochondrial dysfunction related diseases | |
| CN106290527A (en) | A kind of water quality toxicity monitoring method based on anaerobic pretreatment Yu air cathode MFC | |
| Cloherty et al. | Berbamine suppresses intestinal SARS-CoV-2 infection via a BNIP3-dependent autophagy blockade | |
| CN102608305A (en) | Method for detecting insecticide resistance of schistosoma japonicum miracidia to praziquantel in vitro | |
| WO2010081158A3 (en) | Blood test for the detection of cancer | |
| IN2012DN01932A (en) | ||
| Saraswathy et al. | Laboratory acute flaccid paralysis surveillance in Malaysia: a decade of commitment to the WHO global polio eradication initiative | |
| Liang et al. | Measurement of electrolyte concentrations in expressed prostatic secretion and urine from patients with chronic prostatitis and its implications. | |
| Yefang et al. | Surveillance and forecast and emergency management of schistosomiasis prevalence at the focal waters in Dantu district of Zhenjiang city | |
| Chen | Real-Time Monitoring of Viral Replication and Anti-Viral Drug Discovery by Molecular FRET Probes | |
| Gu et al. | Study on schistosomiasis control strategy in Ertan reservoir | |
| Shi et al. | Mitochondrial dysfunction induced by bedaquiline as an anti-Toxoplasma alternative | |
| CN202801610U (en) | Human body acupoint detection circuit | |
| Jianting et al. | GW24-e0236 Effects of 5-aminolevulinic acid mediated sonodynamic therapy on macrophages |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120627 |