CN1419824A - Process for producing Beauveria globisporus non-woven fibric ribbon - Google Patents
Process for producing Beauveria globisporus non-woven fibric ribbon Download PDFInfo
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- CN1419824A CN1419824A CN 02138668 CN02138668A CN1419824A CN 1419824 A CN1419824 A CN 1419824A CN 02138668 CN02138668 CN 02138668 CN 02138668 A CN02138668 A CN 02138668A CN 1419824 A CN1419824 A CN 1419824A
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- 238000000034 method Methods 0.000 title claims description 27
- 241000223679 Beauveria Species 0.000 title abstract 2
- 230000008569 process Effects 0.000 title description 3
- 238000004519 manufacturing process Methods 0.000 claims abstract description 28
- 230000001580 bacterial effect Effects 0.000 claims abstract description 14
- 241001481710 Cerambycidae Species 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims description 32
- 241000751139 Beauveria bassiana Species 0.000 claims description 22
- 239000012530 fluid Substances 0.000 claims description 19
- 239000002609 medium Substances 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 230000002538 fungal effect Effects 0.000 claims description 15
- 239000004745 nonwoven fabric Substances 0.000 claims description 13
- 235000015097 nutrients Nutrition 0.000 claims description 11
- 241000894006 Bacteria Species 0.000 claims description 10
- 238000010899 nucleation Methods 0.000 claims description 10
- HSRJKNPTNIJEKV-UHFFFAOYSA-N Guaifenesin Chemical compound COC1=CC=CC=C1OCC(O)CO HSRJKNPTNIJEKV-UHFFFAOYSA-N 0.000 claims description 9
- 239000013530 defoamer Substances 0.000 claims description 9
- -1 polyoxypropylene ethylene oxide glycerin Polymers 0.000 claims description 9
- 238000001816 cooling Methods 0.000 claims description 8
- 238000012856 packing Methods 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 6
- 238000000855 fermentation Methods 0.000 claims description 6
- 230000004151 fermentation Effects 0.000 claims description 6
- 239000012467 final product Substances 0.000 claims description 6
- 239000001963 growth medium Substances 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 244000068988 Glycine max Species 0.000 claims description 5
- 235000010469 Glycine max Nutrition 0.000 claims description 5
- 238000004458 analytical method Methods 0.000 claims description 5
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- 239000008103 glucose Substances 0.000 claims description 5
- 238000011218 seed culture Methods 0.000 claims description 5
- 235000000346 sugar Nutrition 0.000 claims description 5
- 235000015099 wheat brans Nutrition 0.000 claims description 5
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical class [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 4
- 235000009579 balsamo Nutrition 0.000 claims description 4
- 238000012546 transfer Methods 0.000 claims description 4
- CBCKQZAAMUWICA-UHFFFAOYSA-N 1,4-phenylenediamine Chemical compound NC1=CC=C(N)C=C1 CBCKQZAAMUWICA-UHFFFAOYSA-N 0.000 claims description 3
- 229920001817 Agar Polymers 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 244000061456 Solanum tuberosum Species 0.000 claims description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 230000008859 change Effects 0.000 claims description 3
- 235000021552 granulated sugar Nutrition 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 238000000386 microscopy Methods 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 238000000746 purification Methods 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 238000012807 shake-flask culturing Methods 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 238000012360 testing method Methods 0.000 claims description 3
- 210000003934 vacuole Anatomy 0.000 claims description 3
- 239000012531 culture fluid Substances 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 3
- 230000000249 desinfective effect Effects 0.000 abstract description 2
- 238000009630 liquid culture Methods 0.000 abstract description 2
- 230000000856 effect on pests Effects 0.000 abstract 1
- 238000003912 environmental pollution Methods 0.000 abstract 1
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- 241000233866 Fungi Species 0.000 description 10
- 241000607479 Yersinia pestis Species 0.000 description 9
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- 239000002917 insecticide Substances 0.000 description 4
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- 241000690416 Dendrolimus punctatus Species 0.000 description 1
- 241000223250 Metarhizium anisopliae Species 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A process for preparing non-woven disinfecting strips of Beauveria globisporus includes sampling its bacterial strain, slant culture, liquid culture, production with fermentor, and inoculating to non-woven strips. Its advantages are high and durable effect on pests (such as longicorn), low cost and no environmental pollution.
Description
Technical field
The present invention relates to a kind of novel form production method of disinsection fungal, specifically with a kind of non-woven fabric fungal band production method of separating from beauveria bassiana [Beauveria bassiana (Balsamo) Vuillemin] the RCEF0383 bacterial strain of longicorn.
Background technology
Since the 1950's, though chemical pesticide has played enormous function aspect Pest Control, but the thing followed is serious ecological consequence: as cause of the enhancing of some pest population to the common insecticide resistance, control efficiency descends, and dosage increases; Kill and wound pest natural enemy in a large number, cause that the rampant once again and less important insect of target pest rises to primary pest; Some insecticide residues has influenced existent environment of people to the pollution of environment and food.Because a large amount of and irrational use chemical pesticide cause the residual accumulation of agricultural chemicals, and environment is polluted, the ecological balance is destroyed.Mainly show: beneficial organism is killed and wounded, and the pesticide resistance day of insect rises seriously, persticide residue increase etc. in the agricultural byproducts.
In order to overcome above drawback, the biological control industry develops rapidly.Particularly entomogenous fungi is owing to unique diffusivity being arranged and obtaining people's favor at natural powerful Su Cunli.Existing in the world at present more than 20 kind of fungus insecticide product registered, and main product mostly is beauveria bassiana and Metarhizium anisopliae pulvis, and the object of control is mainly forest insect greenhouse and vegetable-crop pest-insect.China mainly by some half industrialized white muscardine fungi factories, produces white muscardine fungi bacterium powder and high cryptogam, is used for the control of dendrolimus punctatus and corn borer.Because entomogenous fungi mainly relies on the insect cuticle invasion, so but do not have good formulation and using method for borer pest.
Summary of the invention
In order to address the above problem, implement the agricultural sustainable development strategy, the implementation of biological control then is necessary, the production of fungus insecticide also is a very promising industry.The invention provides a kind of beauveria bassiana non-woven fabric fungal band manufacturing technique method that good killing effect is arranged for borer pest.
Realize that the concrete technical solution of above-mentioned purpose is as follows:
Beauveria bassiana non-woven fabric fungal band manufacturing technique method is characterized in that:
A, bacterial classification:
The RCEF0383 bacterial strain separates the beauveria bassiana [Beauveria bassiana (Balsamo) Vuillemin] from a kind of longicorn;
B, primary inclined plane spawn culture:
The bacterial classification inoculation of separation and purification in the PPDA medium slant, is put into 25 ℃ of incubators, cultivated 9～14 days, when treating that mycelia is covered with the inclined-plane and produces spore, change shake-flask culture over to;
C, liquid seeds are cultivated:
In the 500ml triangular flask, pack into the liquid seed culture medium of 200ml, 1.5pa/kg autoclaving 30min is to be cooled to about 40 ℃, inserts slant strains, inoculum concentration is 2 cultured primary inclined plane bacterial classifications of 500ml triangle bottle graft, test tube specification 150mm * 15mm, inoculation is placed on the constant-temperature shaking culture case, 24-26 ℃, 180 rev/mins, cultivate and got final product in 3 days～5 days;
D, liquid fermentation production: adopt three grade fermemtation
(1), first class seed pot production
Get the 70L airlift fermentor, the 50L liquid nutrient medium of packing into, temperature is greater than 95 ℃, and adding defoamer polyoxypropylene ethylene oxide glycerin ether 15ml, 14.7 * 10
4Under the Pa pressure, pressurize 25～40 minutes inserts cultivation, 24-26 ℃, throughput 1: 0.5, pressurize 4.903～7.0845 * 10 with 4 bottles of above-mentioned cultured 500ml shake-flask seeds after the sterilization cooling
4Pa through 60～72 hours aerobic culture, reaches the logarithmic growth after date, inserts the secondary seed jar;
(2), the secondary seed jar is produced
Get the 700L airlift fermentor, the 450L liquid nutrient medium of packing into, temperature is greater than 95 ℃, and adding defoamer polyoxypropylene ethylene oxide glycerin ether 150ml, 14.7 * 10
4Under the Pa pressure, pressurize 25～40min all inserts the secondary seed jar with first class seed pot seed 50L after the sterilization cooling and cultivates 24～26 ℃ of temperature, throughput 1: 0.5, pressurize 4.903～7.0845 * 10
4Pa through 60～72 hours aerobic culture, reaches the logarithmic growth after date, inserts three grades of seeding tanks;
(3), three grades of seeding tank productions
Get the 7T airlift fermentor, the 4.5T liquid nutrient medium liquid of packing into, pH6.0～7.0, the hot water temperature who adds is greater than 95 ℃, and add defoamer polyoxypropylene ethylene oxide glycerin ether 1500ml, feed Steam Heating to 121 ℃, pressurize 30 minutes, be cooled to 24～26 ℃, cultured liquid seeds 500L all inserts with the secondary seed jar, aerobic culture, 1: 0.4～0.5V/Vmin of throughput, speed of agitator 190r/m, tank pressure 4.903～7.0845 * 10
4Pa, incubation time 72～96 hours;
When occurring a large amount of mycelia and liquid in the zymotic fluid and give birth to spore, residual sugar is below 1%, and zymotic fluid is than thickness, and the microscopy mycelia attenuates in addition, no tangible vacuole in the mycelium, does not have the mycelia of obviously rupturing in the zymotic fluid, can put jar;
E, with nonwoven strip behind 1.5Pa/Kg autoclaving 30min, the back ratio in 700 nonwoven strip access 100L zymotic fluids of cooling connects (soaking) and goes into cultured zymotic fluid, at 20～28 ℃, tiling was cultivated 5 days on culturing room's frame of RH95%, after treating to cover with mycelia on the bacterium bar, move into temperature at 20～28 ℃, the culturing room of RH90% continues to cultivate 3～5 days, cover with a large amount of spores on the bacterium bar, air-dry getting final product.
Described slant culture base fluid comprises: potato 200g, peptone 10g, glucose 60g, agar 20g, water 1000ml.
Described liquid seed culture medium comprises: contain 20g glucose, 40g analysis for soybean powder, 0.5gKH in the 1000ml medium
2PO
4, add water after each component is mixed and mend, and transfer pH to 6.3 with saturated NaOH to 1000ml.
Described seeding tank is produced liquid nutrient medium and is comprised: 6% wheat bran liquor, 2% white granulated sugar, 2% analysis for soybean powder and 005%K
2HPO
4, pH6.3.Promptly in the 1000ml medium, contain 20g white granulated sugar sugar, 20g analysis for soybean powder, 0.5gKH
2PO
4, add the wheat bran liquor, promptly in 800ml water, add wheat bran 60g and boiled 30 minutes, one deck filtered through gauze is removed residue, adds water after each component is mixed and mends to 1000ml, and transfer pH to 6.3 with saturated NaOH.
Described defoamer is a polyoxypropylene ethylene oxide glycerin ether, and consumption is 0.03% of a zymotic fluid total amount, and promptly dosage is 15ml among the 50L, adds 150ml in the 500L composts or fertilisers of cultivating, adds 1500ml in the 5T composts or fertilisers of cultivating.
According to claim 1,2,3,4 and 5 described wherein any a beauveria bassiana non-woven fabric fungal band manufacturing technique methods, it is characterized in that: described nonwoven strip is long 60cm, wide 5cm, thick 0.4cm.
Because entomogenous fungi has considerable diffusion effect, ability is deposited in long soil place, unique body wall mode of infection, and pest resistance slower development and easy remarkable advantages such as productions, and the result of toxicity test proves with the beauveria bassiana to be that the fungus insecticide of representative has powerful virulence to borer pest such as corn borer, various longicorn, flatheaded borer etc.As long as select good method of application and correct formulation, just can obtain and be better than other biological or the unrivaled good result of chemical pesticide.
The non-woven fabric fungal band formulation of explained hereafter of the present invention has unique advance: the one, and lasting to the control efficiency of insect.Because the existence of the fungal spore on the nonwoven is active in conidial powder, and fungi can also utilize the product spore of constantly growing of the nutrition on the nonwoven, and the spore viability obviously extends, and helps killing long insects such as longicorn of emergence cycle; The 2nd, nonwoven fungi bacterium bar formulation is very long in the field life-span, is subjected to weather effect less relatively.Spore on the nonwoven mainly is to depend on drift to contact polypide, and relative more with the chance of insect contact, the time that advantage factor occurs in the environment is longer, as long as conditions being possessed will infect insect, is subjected to the amplitude of environmental constraints less; The 3rd, the beauveria bassiana bacterium bar of explained hereafter of the present invention is obvious and stable to sky bovine trunk borer insecticidal effect; The 4th, production technology of the present invention is uncomplicated, process stabilizing, easy-regulating, success rate height.The 5th, small investment is taken up an area of between little, the no three wastes and is inscribed, and is particularly suited for medium-sized and small enterprises and adopts.
Embodiment
Embodiment 1
This beauveria bassiana non-woven fabric fungal band manufacturing technique method comprises five road production processes:
1, bacterial classification:
The RCEF0383 bacterial strain is for separating the beauveria bassiana [Beauveria bassiana (Balsamo) Vuillemin] from longicorn;
2, primary inclined plane spawn culture:
In the PPDA medium slant, every 1000ml medium contains potato 200g, peptone 10g, glucose 60g, agar 20g, water 1000ml with the bacterial classification inoculation of separation and purification; Put into 25 ℃ of incubators, cultivated 10 days, when treating that mycelia is covered with the inclined-plane and produces spore, can change shake-flask culture over to;
3, liquid seeds is cultivated:
In the 500ml triangular flask, the liquid seed culture medium of the 200ml that packs into, 1.5pa/kg autoclaving 30min, to be cooled to about 40 ℃, insert slant strains, inoculum concentration is 2 cultured primary inclined plane bacterial classifications of 500ml triangle bottle graft, test tube specification 150mm * 15mm.Inoculation is placed on the constant-temperature shaking culture case, 24-26 ℃, 180 rev/mins, cultivates and gets final product in 3 days～5 days;
4, liquid fermentation production: this operation is divided into three steps and promptly adopts three grade fermemtation
(1), first class seed pot production
Get the 70L airlift fermentor, add raw material and water by prescription, i.e. 50L liquid nutrient medium, temperature is greater than 95 ℃, and adds defoamer polyoxypropylene ethylene oxide glycerin ether 15ml, 14.7 * 10
4Under the Pa pressure, pressurize 25～40 minutes inserts cultivation, 24-26 ℃, throughput 1: 0.5, pressurize 4.903～7.0845 * 10 with 4 bottles of above-mentioned cultured 500ml shake-flask seeds after the sterilization cooling
4Pa through 60～72 hours aerobic culture, reaches the logarithmic growth after date, inserts the secondary seed jar;
(2), the secondary seed jar is produced
Get the 700L airlift fermentor, calculate, note the condensed water 200L in the deduction disinfecting process to cultivate the 500L liquid spawn; Add raw material and water by prescription, the 450L liquid nutrient medium of promptly packing into, liquid culture based formulas and other controlled condition are produced with the one-level seeding tank; Reach the logarithmic growth after date, insert three grades of seeding tanks.
(3), three grades of seeding tank productions get the 7T airlift fermentor, the 4.5T liquid nutrient medium liquid of packing into, fermentation is produced with the one-level seeding tank with culture medium prescription, pH6.0～7.0.Composts or fertilisers of cultivating is dropped in the fermentation tank in proportion, add hot water, the hot water temperature equals 95 ℃, feed Steam Heating to 121 ℃, pressurize 30 minutes is cooled to 24～26 ℃, and cultured liquid seeds 500L all inserts with the secondary seed jar, volume should be controlled at fermentation tank volume 80% when noting cultivating, be 5000L, aerobic culture, throughput 1: 0.4V/Vmin, speed of agitator 190r/m, tank pressure 4.903～7.0845 * 10
4Pa, incubation time 80 hours; Through being up to the standards, can put jar.
When occurring a large amount of mycelia and liquid in the zymotic fluid and give birth to spore, residual sugar is below 1%, and zymotic fluid is than thickness, and the microscopy mycelia attenuates in addition, no tangible vacuole in the mycelium, does not have the mycelia of obviously rupturing in the zymotic fluid, can put jar;
5, sterilization of bacterium bar and inoculated and cultured
Nonwoven strip is cut into long 60cm, wide 5cm, the strip of thick 0.5cm is behind 1.5Pa/Kg autoclaving 30min, the back ratio in 700 nonwoven strip access 100L zymotic fluids of cooling connects (soaking) and goes into cultured zymotic fluid, at 20～28 ℃, tiling was cultivated 5 days on culturing room's frame of RH95%, after treating to cover with mycelia on the bacterium bar, move into temperature at 20～28 ℃, the culturing room of RH90% continues to cultivate 3～5 days, covers with a large amount of spores on the bacterium bar, air-dry getting final product.
Claims (6)
1, beauveria bassiana non-woven fabric fungal band manufacturing technique method is characterized in that:
A, bacterial classification:
The RCEF0383 bacterial strain separates the beauveria bassiana [Beauveria bassiana (Balsamo) Vuillemin] from longicorn;
B, primary inclined plane spawn culture:
The bacterial classification inoculation of separation and purification in the PPDA medium slant, is put into 25 ℃ of incubators, cultivated 9～14 days, when treating that mycelia is covered with the inclined-plane and produces spore, change shake-flask culture over to;
C, liquid seeds are cultivated:
In the 500ml triangular flask, pack into the liquid seed culture medium of 200ml, 1.5pa/kg autoclaving 30 minutes is to be cooled to about 40 ℃, inserts slant strains, inoculum concentration is 2 cultured primary inclined plane bacterial classifications of 500ml triangle bottle graft, test tube specification 150mm * 15mm, inoculation is placed on the constant-temperature shaking culture case, 24-26 ℃, 180 rev/mins, cultivate and got final product in 3 days～5 days;
D, liquid fermentation production: adopt three grade fermemtation
(1), first class seed pot production
Get the 70L airlift fermentor, the 50L liquid nutrient medium of packing into, temperature is greater than 95 ℃, and adding defoamer polyoxypropylene ethylene oxide glycerin ether 15ml, 14.7 * 10
4Under the Pa pressure, pressurize 25～40 minutes inserts cultivation, 24-26 ℃, throughput 1: 0.5, pressurize 4.903～7.0845 * 10 with 4 bottles of above-mentioned cultured 500ml shake-flask seeds after the sterilization cooling
4Pa through 60～72 hours aerobic culture, reaches the logarithmic growth after date, inserts the secondary seed jar;
(2), the secondary seed jar is produced
Get the 700L airlift fermentor, the 450L liquid nutrient medium of packing into, temperature is greater than 95 ℃, and adding defoamer polyoxypropylene ethylene oxide glycerin ether 150ml, 14.7 * 10
4Under the Pa pressure, pressurize 25～40min all inserts the secondary seed jar with first class seed pot seed 50L after the sterilization cooling and cultivates 24～26 ℃ of temperature, throughput 1: 0.5, pressurize 4.903～7.0845 * 10
4Pa through 60～72 hours aerobic culture, reaches the logarithmic growth after date, inserts three grades of seeding tanks;
(3), three grades of seeding tank productions
Get the 7T airlift fermentor, the 4.5T liquid nutrient medium liquid of packing into, pH6.0～7.0, the hot water temperature who adds is greater than 95 ℃, and add defoamer polyoxypropylene ethylene oxide glycerin ether 1500ml, feed Steam Heating to 121 ℃, pressurize 30 minutes, be cooled to 24～26 ℃, cultured liquid seeds 500L all inserts with the secondary seed jar, aerobic culture, 1: 0.4～0.5V/Vmin of throughput, speed of agitator 190r/m, tank pressure 4.903～7.0845 * 10
4Pa, incubation time 72～96 hours;
When occurring a large amount of mycelia and liquid in the zymotic fluid and give birth to spore, residual sugar is below 1%, and zymotic fluid is than thickness, and the microscopy mycelia attenuates in addition, no tangible vacuole in the mycelium, does not have the mycelia of obviously rupturing in the zymotic fluid, can put jar;
E, with nonwoven strip 1.5Pa/Kg autoclaving 30 minutes, the back ratio in 700 nonwoven strip access 100L zymotic fluids of cooling connects (soaking) and goes into cultured zymotic fluid, at 20～28 ℃, tiling was cultivated 5 days on culturing room's frame of RH95%, after treating to cover with mycelia on the bacterium bar, move into temperature at 20～28 ℃, the culturing room of RH90% continues to cultivate 3～5 days, cover with a large amount of spores on the bacterium bar, air-dry getting final product.
2, beauveria bassiana non-woven fabric fungal band manufacturing technique method according to claim 1, it is characterized in that: described slant culture base fluid comprises: potato 200g, peptone 10g, glucose 40g, agar 20g, water 1000ml.
3, beauveria bassiana non-woven fabric fungal band manufacturing technique method according to claim 1, it is characterized in that: described liquid seed culture medium comprises: contain 20g glucose, 40g analysis for soybean powder, 0.5gKH in the 1000ml medium
2PO
4, add water after each component is mixed and mend, and transfer pH to 6.3 with saturated NaOH to 1000ml.
4, beauveria bassiana non-woven fabric fungal band manufacturing technique method according to claim 1 is characterized in that: described seeding tank is produced liquid nutrient medium and is comprised: as contain 20g white granulated sugar sugar, 20g analysis for soybean powder, 0.5gKH in the 1000ml medium
2PO
4, add the wheat bran liquor, promptly in 800m1 water, add wheat bran 60g and boiled 30 minutes, one deck filtered through gauze is removed residue, adds water after each component is mixed and mends to 1000ml, and transfer pH to 6.3 with saturated NaOH.
5, beauveria bassiana non-woven fabric fungal band manufacturing technique method according to claim 1, it is characterized in that: described defoamer is a polyoxypropylene ethylene oxide glycerin ether, consumption is 0.03% of culture fluid (zymotic fluid) total amount, be that dosage is 15ml among the 50L, add 150ml in the 500L composts or fertilisers of cultivating, add 1500ml in the 5T composts or fertilisers of cultivating.
6, according to claim 1,2,3,4 or 5 described wherein any a beauveria bassiana non-woven fabric fungal band manufacturing technique methods, it is characterized in that: described nonwoven strip is long 60cm, wide 5cm, thick 0.4cm.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02138668 CN1212773C (en) | 2002-11-22 | 2002-11-22 | Process for producing Beauveria globisporus non-woven fibric ribbon |
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|---|---|---|---|
| CN 02138668 CN1212773C (en) | 2002-11-22 | 2002-11-22 | Process for producing Beauveria globisporus non-woven fibric ribbon |
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| CN1212773C CN1212773C (en) | 2005-08-03 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101967463A (en) * | 2010-11-09 | 2011-02-09 | 无锡益鹏集团有限公司 | Method for improving spore germination rate of beauveria bassiana by adding nutrients |
| CN101422170B (en) * | 2008-10-28 | 2011-12-28 | 云南省烟草科学研究所 | Streptomyces globisporus microbial agent for controlling Alternaria alternate and preparation method thereof |
| CN102304478A (en) * | 2011-08-30 | 2012-01-04 | 江南大学 | Method for improving heat resistance in spores of Beauveria bassiana (Balsamo) Vuillemin and use of spores of Beauveria bassiana (Balsamo) Vuillemin |
| CN102329837A (en) * | 2011-09-01 | 2012-01-25 | 安徽农业大学 | Preparation method of Beauveria sp. extract with functions of tyrosinase inhibitor and antioxidant |
| CN106350457A (en) * | 2016-08-25 | 2017-01-25 | 福建省林业科学研究院 | Preparation method of high sporopollen of beauveria bassiana |
| CN108378063A (en) * | 2018-05-18 | 2018-08-10 | 中国农业科学院农业环境与可持续发展研究所 | A kind of preparation method of muscardine granule |
| CN114621908A (en) * | 2022-03-16 | 2022-06-14 | 福建省林业科学研究院 | Fermentation method of beauveria bassiana serosa |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102217592A (en) * | 2011-04-26 | 2011-10-19 | 福建省林业科学研究院 | Fungus sticking paste and making method and application of the fungus sticking paste |
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2002
- 2002-11-22 CN CN 02138668 patent/CN1212773C/en not_active Expired - Lifetime
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101422170B (en) * | 2008-10-28 | 2011-12-28 | 云南省烟草科学研究所 | Streptomyces globisporus microbial agent for controlling Alternaria alternate and preparation method thereof |
| CN101967463A (en) * | 2010-11-09 | 2011-02-09 | 无锡益鹏集团有限公司 | Method for improving spore germination rate of beauveria bassiana by adding nutrients |
| CN101967463B (en) * | 2010-11-09 | 2012-01-04 | 无锡益鹏集团有限公司 | Method for improving spore germination rate of beauveria bassiana by adding nutrients |
| CN102304478A (en) * | 2011-08-30 | 2012-01-04 | 江南大学 | Method for improving heat resistance in spores of Beauveria bassiana (Balsamo) Vuillemin and use of spores of Beauveria bassiana (Balsamo) Vuillemin |
| CN102304478B (en) * | 2011-08-30 | 2013-04-17 | 江南大学 | Method for improving heat resistance in spores of Beauveria bassiana (Balsamo) Vuillemin and use of spores of Beauveria bassiana (Balsamo) Vuillemin |
| CN102329837A (en) * | 2011-09-01 | 2012-01-25 | 安徽农业大学 | Preparation method of Beauveria sp. extract with functions of tyrosinase inhibitor and antioxidant |
| CN106350457A (en) * | 2016-08-25 | 2017-01-25 | 福建省林业科学研究院 | Preparation method of high sporopollen of beauveria bassiana |
| CN106350457B (en) * | 2016-08-25 | 2019-08-13 | 福建省林业科学研究院 | A kind of preparation method of the high cryptogam of muscardine |
| CN108378063A (en) * | 2018-05-18 | 2018-08-10 | 中国农业科学院农业环境与可持续发展研究所 | A kind of preparation method of muscardine granule |
| CN114621908A (en) * | 2022-03-16 | 2022-06-14 | 福建省林业科学研究院 | Fermentation method of beauveria bassiana serosa |
| CN114621908B (en) * | 2022-03-16 | 2024-03-22 | 福建省林业科学研究院 | Fermentation method of beauveria bassiana serosa |
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