CN1405296A - Dynamic space cell culture system - Google Patents
Dynamic space cell culture system Download PDFInfo
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- CN1405296A CN1405296A CN 02145050 CN02145050A CN1405296A CN 1405296 A CN1405296 A CN 1405296A CN 02145050 CN02145050 CN 02145050 CN 02145050 A CN02145050 A CN 02145050A CN 1405296 A CN1405296 A CN 1405296A
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Abstract
Dynamic space cell culture system comprises: the cell culturing room, liquor storing room and peristalsis pump. The cell culturing room is made up of several two-way laminar flow pervasion film cell culturing units. The liquor storing room is made up of liquor storing units with the same number. The liquor storing unit is separated into nutrition antrum and recycles antrum. It forms several cell culturing subsystem which has fixed flux and low velocity of flow through conduits, peristalsis pump and cell culturing units. One of the cell culturing subsystem has a cell sample fixing device.
Description
Technical field
The present invention relates to the space biotechnology, be meant a kind of dynamic cellular culture systems that can carry out the various kinds of cell cultivation in the space for a long time simultaneously especially.
Background technology
Under the space microgravity environment, gravity settling is tending towards disappearing, for cell three-dimensional growth and cell high-density culture have been created unprecedented condition and chance.Therefore, the spatial cell cultivation is the important content of present various countries space life science experiment.Spatial cell is cultivated and to be needed nutrition supply, can be divided into static cultivation and dynamically cultivates according to the difference of nutritive medium supply mode.Static cultivation is exactly to preset a certain amount of nutritive medium and cell in huge sealing culturing room, can not change nutritive medium in the working process.Dynamically cultivate and be meant that nutritive medium can supply with continuously, supply with fresh nutrient solution to cell continuously, and reclaim meta-bolites.Static cultivation is along with the prolongation of incubation time, and the nutritive medium spatial distribution will be inhomogeneous, and the intensive place of local cells causes cell to be in starvation even death owing to lacking essential nutritive medium.Therefore, static cultivation is not suitable for carrying out long-term cell cultures.The residing nutrient environment of each stage cell of static cultivation has great difference simultaneously, can produce some uncertain factors, is unfavorable for biological study.Dynamic cultivation can guarantee to make in whole cell cultivation process cell to be in all the time and obtain competent fresh nutrient solution, reduced uncertain factor, improved the utilization ratio of nutritive medium simultaneously greatly, makes the cell spaces long-term cultivation become possibility.Dynamic cultivation the earliest is in the later stage eighties, and Switzerland and Germany have developed a kind of cell culture system that has two culturing room, and one is the static cultivation chamber, and another is dynamic culturing room.This dynamic culturing room is a kind of structure that can supply with fresh nutrient solution to cell continuously of utilizing the osmotic pump principle development.This culturing room's volume is 200 μ l, and fresh nutrient solution warehouse volume is 230 μ l, and self-driven osmotic pump is supplied to cell with the flow velocity of 1 μ l/h with fresh nutrient solution.China also developed the dynamic cellular culture apparatus in 1994, also adopted self-driven osmotic pump, feed flow flow 70 μ l/ days; The cell culture chamber volume is 4ml.But above-mentioned two kinds of dynamic cellular culture apparatuses are owing to be subjected to the restriction of osmotic pump flow velocity, and the utility appliance of osmotic pump comparatively expands, and can not be fit to jumbo multiple spatial cell and cultivate simultaneously.Airship is when returning ground in addition, and the state of cell tends to change, and can the pair cell spatiality carry out stationary device and existing spatial cell culture systems shortage is a kind of, is used to the cell spaces state to carry out biological study.
Summary of the invention
Therefore, purpose of the present invention is exactly will provide a kind of can carry out the large vol of various kinds of cell cultivation, dynamic space cell culture system over a long time simultaneously.This system also has a cell sampling solidification equipment simultaneously, and the biological study of spatial cell state is provided for the biologist.
The object of the present invention is achieved like this, and the dynamic space cell culture system comprises: cell culture chamber, fluid reservoir, peristaltic pump.
Cell culture chamber is made up of a plurality of independently two-way laminar flow permeable membrane cell culture units.Its middle two parallel permeable membranes, lumen of formation culturing cell of being equipped with of said two-way laminar flow permeable membrane cell culture unit.Constitute two mobile nutritive medium cavitys up and down up and down between two parallel permeable membranes and the cell-wall.
Fluid reservoir is by forming with the liquid storage unit of cell culture unit same number.Said liquid storage unit is separated into the nutrition sap cavity and is reclaimed sap cavity by the upset film.
The passage number of peristaltic pump is identical with the cell culture unit number, by pipeline, peristaltic pump and cell culture unit, the nutrition sap cavity is connected with the recovery sap cavity, form a plurality of cell cultures subsystems that independently have the continuous perfusion of constant current amount, miniflow speed, can carry out various kinds of cell simultaneously and cultivate.One of them cell cultures subsystem also has a cell sampling solidification equipment.
The cell sampling solidification equipment is deposited the curing chamber and the compensation sap cavity of solidifying agent and is formed by ram pump.Ask for an interview the inventor's Chinese patent in detail, name is called: spatial cell state solidification equipment, the patent No. is: 02261148.7.
Peristaltic pump is driven by stepper-motor, and driving power adopts four of reduce power consumption single four to clap narrow pulsewidth driving circuit mutually, is made up of multivibrator, time sequential pulse divider, pulse width control circuit and multichannel analog line driver.This pattern greatly reduces motor power consumption, can provide enough static torques again, and its maximum static torque is 34.3Ncm, and power consumption is 1.3W.
Advantage of the present invention is:
1. this system can be under space environment, simultaneously, for a long time, large vol carries out various kinds of cell and cultivates;
2. this system has compact construction, and is low in energy consumption.
3. this system can fix the part cell in face of airship returns ground, keeps the spatiality of cell, is used to the cell spaces state to carry out biological study.
Description of drawings
Fig. 1 is a dynamic space cell culture system structural representation;
Fig. 2 is two-way laminar flow permeable membrane cell culture unit structural representation;
Fig. 3 is the unitary structural representation of liquid storage;
Fig. 4 is a cell sampling solidification equipment structural representation;
Fig. 5 is stepper motor driving circuit figure.
Embodiment
Further the present invention is elaborated below in conjunction with drawings and Examples, present embodiment has four cell cultures subsystems, as shown in Figure 1.
Cell culture chamber 1 is made up of four two-way laminar flow permeable membrane cell culture units 2, and this unit adopts polycarbonate to be pressed into rectangle, and the centre is equipped with the lumen 22 that two parallel permeable membranes 21 constitute culturing cell, and capacity is 10ml.Between two permeable membranes 21 and the cell-wall up and down two mobile nutritive medium cavitys 23 up and down, each cavity is equipped with feed liquor port 24 and fluid port 25 respectively, and the feed liquor port 24 of two cavitys 23 and fluid port 25 position opposite, thereby make the nutritive medium flow direction of two cavitys opposite, see Fig. 2.Cell culture chamber 1 is put having and is kept on the temperature required semiconductor cooler of culturing cell 28.The fluid port 25 of nutritive medium cavity 23 is connected with the input port 47 that reclaims sap cavity 42 by pipeline 7, and feed liquor port 24 is connected with the output port 46 of peristaltic pump 5, nutrition sap cavity 41 by pipeline 7, sees Fig. 3.The flow velocity of every road pipeline is 0.5ml/ hour, by the pipeline peristaltic pump fresh nutrient solution is flowed to cell culture unit 2, and will reclaim liquid and take back recovery sap cavity 42.There is a cell culture unit 2 to have cell sampling solidification equipment 6 in four above-mentioned cell culture units.Unitary lumen 22 sidewalls of cultivation that have the cell sampling solidification equipment are equipped with sampling port 26 and compensation liquid port 27, see Fig. 4.
Fluid reservoir 3 is made up of four liquid storage unit 4, and each liquid storage unit 4 is by having upset film 43 to be divided into nutrition sap cavity 41 in the middle of left half cavity 44 and right half chamber 45 and reclaiming sap cavity 42.Left half cavity is identical with right half cavity shape.Shape approximation half cavity shape of upset film 43, volume is slightly less than half chamber.The mouth edge of upset film 43 about on the half accent limit, the centre have a sealing fill out circle 49 by be spirally connected with about half chamber and upset film phonograph seal fix.The upset film is made by flexible rubber material.When the experiment beginning, nutritive medium all is contained in nutrition sap cavity 41, and it is empty reclaiming sap cavity 42, recessed half chamber 45 to the right of upset film, and the volume of nutrition sap cavity 41 is the unitary volume of whole liquid storage, is full of nutritive medium in it, and it is empty reclaiming sap cavity 42, and its volume is zero.Along with nutrition sap cavity 41 is constantly exported nutritive medium, reclaim liquid and flow back to recovery sap cavity 42, the upset film begins to left half cavity recessed, and nutrition sap cavity 41 volumes constantly dwindle, and until the whole emptyings of nutritive medium, nutrition sap cavity 41 volumes are zero.At this moment reclaim liquid and be full of liquid storage unit 4, nutrition sap cavity 41 volumes are zero.In the whole process, the unitary cumulative volume of liquid storage remains unchanged, and has made full use of the liquid storage space, makes cumulative volume reduce half, and realizes a hermetic liquid process of changing, and is fit to the space and uses.
Fluid reservoir 3 is put having and is kept on the temperature required semiconductor cooler of nutritive medium 44.
Cell sampling solidification equipment 6 comprises: ram pump 61, solidifying agent chamber 62, compensation sap cavity 63.The top port 613 of ram pump is connected with the sampling port 26 of lumen 22 by pipeline 69, and compensation sap cavity 63 is connected with the fluid infusion port 27 of lumen 22 by pipeline 69, and solidifying agent chamber 62 is connected with the side port 612 of ram pump by pipeline.
The working process of cell sampling solidification equipment is as follows: original state, and solidifying agent chamber 62 and compensation sap cavity 63 are loaded onto a certain amount of solidifying agent and nutritive medium respectively; Piston 611 is in closing condition in the ram pump 61, and the spring 612 on the piston is in impaction state, and locks with tommy 67, and turn-Off tool clamping 68 flattens pipeline 69.Be full of cell and nutritive medium in the lumen 22, when ground send solidify open instruction after, curing motor 68 begins to pull pin and follows closely 67, turn-Off tool clamping 68 displacements, pipeline 69 is unimpeded, and spring 612 unclamps, and piston glides, utilize pressure difference that solidifying agent and enchylema are made by oneself and be evacuated to the ram pump cavity, nutritive medium is delivered to lumen 22 from compensated cavity 63.When turn-Off tool clamping 68 moved to travel switch 64, piston base pressed travel switch 66, solidified motor and began counter-rotating, pinned piston once more, when moving to travel switch 65, turn-offed and solidified motor, and cell sampling solidifies and finishes.
Dynamic space cell culture system of the present invention successfully is loaded in No. three airship return capsules of divine boat, has carried out spatial cell and has cultivated scientific experiment, and reached biologist's expected effect.
Claims (2)
1. dynamic space cell culture system comprises: cell culture chamber (1), and fluid reservoir (3), peristaltic pump (5) is characterized in that:
Cell culture chamber (1) is made up of a plurality of independently two-way laminar flow permeable membrane cell culture units (2); The centre of said two-way laminar flow permeable membrane cell culture unit is equipped with two parallel permeable membranes (21), constitute the lumen (22) of culturing cell, constitute two mobile nutritive medium cavitys (23) up and down up and down between two parallel permeable membranes (21) and the cell culture unit wall; Cell culture chamber (1) has keeps the temperature required semiconductor cooler of culturing cell (28);
Fluid reservoir (3) is by forming with the liquid storage unit (4) of cell culture unit (2) same number; Each liquid storage unit (4) is by having upset film (43) to be divided into nutrition sap cavity (41) in the middle of left half cavity (44) and right half chamber (45) and reclaiming sap cavity (42); Nutrition sap cavity (41) has a nutrient input outbound port 46; Reclaim sap cavity (42) and receive liquid input port 47 once; Left half cavity is identical with right half cavity shape; The shape approximation left half cavity or right half chamber of upset film (43), volume is slightly less than left half cavity or right half chamber; Upset film (43) mouthful edge about on the half accent limit, the centre have a sealing fill out circle (48) by be spirally connected with about half chamber and the film phonograph seal that overturns fix; The upset film is made by the elastomeric material of flexibility; Fluid reservoir (3) is put having and is kept on the temperature required semiconductor cooler of nutritive medium (44);
Passage (51) number of peristaltic pump (5) is identical with cell culture unit (2) number, and peristaltic pump (5) has stepper-motor (52);
The said nutritive medium cavity (23) that flows is equipped with feed liquor port (24) and fluid port (25) respectively, the feed liquor port (24) and fluid port (25) position opposite of two mobile cavitys (23) of nutritive mediums, fluid port (25) is connected with recovery liquid input port (47) by pipeline (7), feed liquor port (24) is connected with the nutrient input outbound port (46) of peristaltic pump (5), fluid reservoir (3) by pipeline (7), forms a plurality of independently cell cultures subsystems; One of them cell cultures subsystem also has a cell sampling solidification equipment (6); The lumen of the cell culture unit of this subsystem (22) sidewall is equipped with sampling port (26) and compensation liquid port (27).
2. according to claim 1 dynamic cellular culture systems, it is characterized in that: said driving source for step motor adopts four single four to clap narrow pulsewidth driving mutually, and this power supply is made up of multivibrator (521), time sequential pulse divider (522), pulse width control circuit (523) and multichannel analog line driver (524); Multivibrator (521) is by four or two input nand gate U1, resistance R 1, and R2 and capacitor C 1, C2 forms; Time sequential pulse divider (522) is by rest-set flip-flop U2, and U3 forms; Pulse width control circuit (523) is by capacitor C 3, C4, and C5, C6 forms; Multichannel analog line driver (524) is made up of Darlington driving mechanism U4.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021450501A CN1177033C (en) | 2002-11-05 | 2002-11-05 | Dynamic space cell culture system |
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| CNB021450501A CN1177033C (en) | 2002-11-05 | 2002-11-05 | Dynamic space cell culture system |
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| CN1405296A true CN1405296A (en) | 2003-03-26 |
| CN1177033C CN1177033C (en) | 2004-11-24 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101985600A (en) * | 2010-11-09 | 2011-03-16 | 北京理工大学 | Turnover membrane bioreactor for use in micro-gravity environment |
| CN102181364A (en) * | 2011-03-11 | 2011-09-14 | 北京航空航天大学 | Device for culturing in vitro cells through single axis rotating and online shearing |
| WO2013155962A1 (en) * | 2012-04-18 | 2013-10-24 | 广州洁特生物过滤制品有限公司 | High throughput cell incubator and manufacturing method thereof |
| CN105627976A (en) * | 2016-02-24 | 2016-06-01 | 孟红琳 | Solidification-type flatness sampling device for constructional engineering |
| CN105670929A (en) * | 2016-03-14 | 2016-06-15 | 江苏大学 | Micro-fluidic experimental device and method for cell culture condition optical control |
| CN107532119A (en) * | 2015-04-22 | 2018-01-02 | 伯克利照明有限公司 | Culture station for microfluidic device |
| CN108641943A (en) * | 2018-04-10 | 2018-10-12 | 中国科学院上海技术物理研究所 | One kind being used for space embryonic development and status saver |
| CN114317395A (en) * | 2021-12-31 | 2022-04-12 | 中国肉类食品综合研究中心 | Adherent cell large-scale culture process |
| TWI777599B (en) * | 2021-06-04 | 2022-09-11 | 國立中興大學 | cell grabbing device |
-
2002
- 2002-11-05 CN CNB021450501A patent/CN1177033C/en not_active Expired - Fee Related
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101985600B (en) * | 2010-11-09 | 2012-12-26 | 北京理工大学 | Turnover membrane bioreactor for use in micro-gravity environment |
| CN101985600A (en) * | 2010-11-09 | 2011-03-16 | 北京理工大学 | Turnover membrane bioreactor for use in micro-gravity environment |
| CN102181364A (en) * | 2011-03-11 | 2011-09-14 | 北京航空航天大学 | Device for culturing in vitro cells through single axis rotating and online shearing |
| CN102181364B (en) * | 2011-03-11 | 2013-01-30 | 北京航空航天大学 | Device for culturing in vitro cells through single axis rotating and online shearing |
| WO2013155962A1 (en) * | 2012-04-18 | 2013-10-24 | 广州洁特生物过滤制品有限公司 | High throughput cell incubator and manufacturing method thereof |
| CN107532119A (en) * | 2015-04-22 | 2018-01-02 | 伯克利照明有限公司 | Culture station for microfluidic device |
| CN105627976B (en) * | 2016-02-24 | 2018-03-16 | 温岭市创嘉信息科技有限公司 | Architectural engineering cured type flatness sampler |
| CN105627976A (en) * | 2016-02-24 | 2016-06-01 | 孟红琳 | Solidification-type flatness sampling device for constructional engineering |
| CN105670929A (en) * | 2016-03-14 | 2016-06-15 | 江苏大学 | Micro-fluidic experimental device and method for cell culture condition optical control |
| CN108641943A (en) * | 2018-04-10 | 2018-10-12 | 中国科学院上海技术物理研究所 | One kind being used for space embryonic development and status saver |
| TWI777599B (en) * | 2021-06-04 | 2022-09-11 | 國立中興大學 | cell grabbing device |
| CN114317395A (en) * | 2021-12-31 | 2022-04-12 | 中国肉类食品综合研究中心 | Adherent cell large-scale culture process |
| CN114317395B (en) * | 2021-12-31 | 2024-04-02 | 中国肉类食品综合研究中心 | Large-scale culture process for adherent cells |
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| CN1177033C (en) | 2004-11-24 |
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