CN1400299A - Method for continuously extracting eucommia oil and aucuboside from eucommia seed - Google Patents
Method for continuously extracting eucommia oil and aucuboside from eucommia seed Download PDFInfo
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- CN1400299A CN1400299A CN 01128744 CN01128744A CN1400299A CN 1400299 A CN1400299 A CN 1400299A CN 01128744 CN01128744 CN 01128744 CN 01128744 A CN01128744 A CN 01128744A CN 1400299 A CN1400299 A CN 1400299A
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Abstract
The present invention relates to a method for continuously extracting biological active component by using eucommia seed as raw material, including (A). utilizing supercritical pure CO2 or high-purity CO2 to extract high linolenic acid eucommia oil from eucommia kernel dried powder; and (B). using water, methyl alcohol or ethyl alcohol as extracting agent, and adopting the processes of heating extraction, reduced pressure concentration, macroporous resin separation and column chromatography to extract aucuboside from residual cake in which the eucommia oil is extracted. Said invention is simple in process, low in cost, high in product yield and good in quality.
Description
Content of the present invention belongs to biochemical article preparing technical field, relates to a kind of method of extracting health care and medical activeconstituents from eucommia bark seed.
The bark of eucommia (Eucommia ulmoides) is a kind of famous and precious medicinal material that is distributed in China western part to middle part, always is used as medicine with its bark on the traditional Chinese medicine.In eucommia bark seed (benevolence), contain more rich linolenic oil composition, unsaturated fatty acid content is high in this linolenic oil, reach 91%, wherein reach more than 73% for the necessary lipid acid of human body (linoleic acid plus linolenic acid), and more outstanding be its linolenic acid content up to 61%, this be other grease can not compare.But linolenic acid metabolism in human body is EPA (EPA), then metabolism is docosahexenoic acid (DHA), studies confirm that the effect that EPA has blood fat reducing and thrombocyte to solidify in a large number, thereby diseases such as energy prevention of brain thrombus and myocardial infarction, DHA has and promotes nervous system development, improves learning and memory function and retinal reflex ability, prevention senile dementia and effects such as cancer and agaist allergic symptoms, thereby linolenic acid is particularly useful for health care for the middle and old aged and promotes juvenile's brain development and strengthen aspect such as ability of learning and memory.At present this area method of being used to obtain Eucommia Oil is continued to use traditional extraction always, exist have in the oil product that residual solvent, oil colours are muddy, of poor quality, the product yield hangs down particularly defectives such as linolenic acid content is low.In addition, in extracting the slag dregs of rice of Eucommia Oil, also contain abundant rhinanthin composition, this is a kind of important source material of making healthcare products and medicine, have multiple efficacies such as antisepsis and anti-inflammation, but this area is not seen as yet by the report that extracts rhinanthin technology in the slag dregs of rice that extract Eucommia Oil up to now, cause this part useful resources to be discarded in vain, reduced the economic worth of eucommia bark seed.
The objective of the invention is to provide a kind of method of from eucommia bark seed, extracting Eucommia Oil and rhinanthin continuously at the existing problem of this area prior art.
Extracting method of the present invention comprises from the bark of eucommia benevolence method of extracting rhinanthin the method for extracting Eucommia Oil and the slag dregs of rice after extracting Eucommia Oil, and its concrete extraction step is:
The extraction of a, Eucommia Oil---the bark of eucommia benevolence of shelling is pulverized the back cross 25～30 mesh sieves, powder will sieve afterwards at 60～90 ℃ of inner dryings, be that 10～35MPa, flow velocity are the overcritical pure CO of 250～5000ml/min with force value in 20～80 ℃ of temperature ranges then
2Or high-purity CO
2Extracted 0.5～2 hour, and on separator column, separated after the extract step-down, promptly obtain linolenic acid content and be 60%～65% Eucommia Oil.
The extraction of b, rhinanthin---will extract the Eucommia Oil slag dregs of rice afterwards and in 50～90 ℃ of temperature ranges, extract 3 times with extracting solution, extracting solution is evaporated to 1/5～1/10 of extracting liquid volume, concentrated solution is gone up macroporous resin column through activated carbon decolorizing and is separated after 30～60 minutes, and then makes rhinanthin with chromatography.
Extract the method for Eucommia Oil with existing extraction and compare the overcritical pure CO of usefulness of the present invention
2Or high-purity CO
2The bark of eucommia oil colours that extracts is light, limpid, noresidue solvent, oil quality height, uses high-purity supercritical CO
2Extraction bark of eucommia benevolence also can improve the linolenic acid content in the oil product; In addition, the present invention also adopts series matching technology further to extract rhinanthin from the slag dregs of rice that extract after the Eucommia Oil, its extracting method is simple, cost is low, the yield height of rhinanthin, purity height, improve the comprehensive utilization ratio that has health care and medical activeconstituents in the bark of eucommia raw material greatly, increased the economic worth of eucommia bark seed.
Below with reference to specific embodiment content of the present invention is described further.
One, the extraction of high linolenic Eucommia Oil
To shell and chippy bark of eucommia benevolence powder is crossed 30 mesh sieves, the powder that will sieve is at 60～90 ℃ of inner dryings, is that 35MPa, flow velocity are the high-purity overcritical pure CO of 2500ml/min with force value under 80 ℃ of temperature then
2Extracted 2 hours, and separated on separator column after the extract step-down, promptly obtain faint yellow, limpid Eucommia Oil, the Eucommia Oil yield is 20%～30%, and linolenic acid content is 60%～65%.
Two, the extraction of rhinanthin
With 20kg extracted the slag dregs of rice water after the Eucommia Oil, 20%～80% methyl alcohol or ethanol extracts 3 times in 50～90 ℃ of temperature, extracting solution is evaporated to 1/5 of extracting liquid volume, concentrated solution is gone up macroporous resin (as D4020, D201, s-8 etc.) post through activated carbon decolorizing and is separated after 40 minutes, and then with chromatographic column (for example silica gel, aluminum oxide, Mierocrystalline cellulose etc.) refining rhinanthin 0.12～0.16kg, rhinanthin yield 0.6%～0.9%, purity 80%～95%.
Claims (4)
1, a kind of method of extracting Eucommia Oil and rhinanthin from eucommia bark seed continuously is characterized in that:
The extraction of a, Eucommia Oil---the bark of eucommia benevolence of shelling is pulverized the back cross 25～30 mesh sieves, powder will sieve afterwards at 60～90 ℃ of inner dryings, be that 10～35MPa, flow velocity are the overcritical pure CO of 250～5000ml/min with force value in 20～80 ℃ of temperature ranges then
2Or high-purity CO
2Extracted 0.5～2 hour, and on separator column, separated after the extract step-down, promptly obtain linolenic acid content and be 60～65% Eucommia Oil.
The extraction of b, rhinanthin---will extract the Eucommia Oil slag dregs of rice afterwards and in 50～90 ℃ of temperature, extract 3 times with extracting solution, extracting solution is evaporated to 1/5～1/10 of extracting liquid volume, concentrated solution is gone up macroporous resin column through activated carbon decolorizing and is separated after 30～60 minutes, and then makes rhinanthin with chromatography.
2, extracting method as claimed in claim 1, the extracting solution that it is characterized in that being used to extracting rhinanthin is a water liquid.
3, extracting method as claimed in claim 1, it is characterized in that being used to extracting rhinanthin extracting solution for being the liquid of extraction agent with 20%～80% methyl alcohol.
4, extracting method as claimed in claim 1, it is characterized in that being used to extracting rhinanthin extracting solution for being the liquid of extraction agent with ethanol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01128744 CN1227341C (en) | 2001-08-06 | 2001-08-06 | Method for continuously extracting eucommia oil and aucuboside from eucommia seed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01128744 CN1227341C (en) | 2001-08-06 | 2001-08-06 | Method for continuously extracting eucommia oil and aucuboside from eucommia seed |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1400299A true CN1400299A (en) | 2003-03-05 |
| CN1227341C CN1227341C (en) | 2005-11-16 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01128744 Expired - Fee Related CN1227341C (en) | 2001-08-06 | 2001-08-06 | Method for continuously extracting eucommia oil and aucuboside from eucommia seed |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100344602C (en) * | 2005-11-08 | 2007-10-24 | 湘西自治州和益生物科技有限公司 | Technological method of continuous extracting effective components in eucommia ulmoides kernel by super critical carbon dioxide |
| CN101002825B (en) * | 2007-01-11 | 2011-06-22 | 西北农林科技大学 | Method for preparing Duzhong soft capsule rich in alpha-linolenic acid and effective component of bark of eucommia |
| CN102504944A (en) * | 2011-11-21 | 2012-06-20 | 广元亿明生物科技有限公司 | Extraction method of eucommia seed oil |
| CN102599270A (en) * | 2012-03-27 | 2012-07-25 | 河南大学 | Health flavoring oil being rich in alpha-linolenic acid and preparation method thereof |
| CN104403798A (en) * | 2014-11-22 | 2015-03-11 | 河南恒瑞源实业有限公司 | Preparation method of eucommia ulmoides essential oil blend |
| CN105950287A (en) * | 2016-07-01 | 2016-09-21 | 国家林业局泡桐研究开发中心 | Extracting and refining technology of eucommia seed oil |
| CN107586605A (en) * | 2017-09-14 | 2018-01-16 | 郑州轻工业学院 | Grease in seed of bark of eucommia, protein, the combined extraction method of Aucubin and gutta-percha |
| CN109329943A (en) * | 2018-11-12 | 2019-02-15 | 汉中市汉鲵食品加工有限公司 | A kind of sustained-release dropping pill of the oil containing giant salamander |
-
2001
- 2001-08-06 CN CN 01128744 patent/CN1227341C/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100344602C (en) * | 2005-11-08 | 2007-10-24 | 湘西自治州和益生物科技有限公司 | Technological method of continuous extracting effective components in eucommia ulmoides kernel by super critical carbon dioxide |
| CN101002825B (en) * | 2007-01-11 | 2011-06-22 | 西北农林科技大学 | Method for preparing Duzhong soft capsule rich in alpha-linolenic acid and effective component of bark of eucommia |
| CN102504944A (en) * | 2011-11-21 | 2012-06-20 | 广元亿明生物科技有限公司 | Extraction method of eucommia seed oil |
| CN102504944B (en) * | 2011-11-21 | 2013-03-20 | 广元亿明生物科技有限公司 | Extraction method of eucommia seed oil |
| CN102599270A (en) * | 2012-03-27 | 2012-07-25 | 河南大学 | Health flavoring oil being rich in alpha-linolenic acid and preparation method thereof |
| CN104403798A (en) * | 2014-11-22 | 2015-03-11 | 河南恒瑞源实业有限公司 | Preparation method of eucommia ulmoides essential oil blend |
| CN104403798B (en) * | 2014-11-22 | 2020-12-22 | 河南恒瑞源实业有限公司 | Preparation method of eucommia ulmoides mixed essential oil |
| CN105950287A (en) * | 2016-07-01 | 2016-09-21 | 国家林业局泡桐研究开发中心 | Extracting and refining technology of eucommia seed oil |
| CN105950287B (en) * | 2016-07-01 | 2019-05-10 | 国家林业局泡桐研究开发中心 | A kind of eucommia ulmoides seed oil extraction refinery practice |
| CN107586605A (en) * | 2017-09-14 | 2018-01-16 | 郑州轻工业学院 | Grease in seed of bark of eucommia, protein, the combined extraction method of Aucubin and gutta-percha |
| CN107586605B (en) * | 2017-09-14 | 2020-07-24 | 郑州轻工业学院 | Comprehensive extraction method of oil, protein, aucubin and gutta-percha in eucommia seeds |
| CN109329943A (en) * | 2018-11-12 | 2019-02-15 | 汉中市汉鲵食品加工有限公司 | A kind of sustained-release dropping pill of the oil containing giant salamander |
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| Publication number | Publication date |
|---|---|
| CN1227341C (en) | 2005-11-16 |
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