CN1396123A - Preparing process and application of composite biologic water cleaner containing living bacteria - Google Patents
Preparing process and application of composite biologic water cleaner containing living bacteria Download PDFInfo
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- CN1396123A CN1396123A CN 01113387 CN01113387A CN1396123A CN 1396123 A CN1396123 A CN 1396123A CN 01113387 CN01113387 CN 01113387 CN 01113387 A CN01113387 A CN 01113387A CN 1396123 A CN1396123 A CN 1396123A
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- compound biological
- live bacteria
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- water purifiers
- biological water
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Abstract
A biocleaner containing living bacteria for cleaning the water in the pool used to raise aquatic products is prepared through uniformly mixing the wet matant strains No, 1,2,3, and 4 of Bacillus subtilis with cellulose and filler, baking and tabletting. It is thrown to the pool water for cleaning it.
Description
Technical field
The present invention relates to a kind of preparation and application of compound biological water purifiers with live bacteria, belong to biological water purification technology field.
Background technology
In recent years, aquaculture develops rapidly.A large amount of sanitary sewages comprise that human and animal excreta, washing water enter river, lake, make in the water in lake, river to contain ammonia nitrogen and nitrate.The Chi Shui of aquaculture pond takes from river, lake, so Chi Shuizhong also contains ammonia nitrogen and nitrate.In the aquaculture process, the movement of aquatic products and superfluous feed cause the pond water eutrophication, and some algae and bacterium breed in a large number, and aquatic products such as fish, crab, shrimp are caused a disease dead, bring loss for the aquatic products aquaculture.
A kind of technology of utilizing microbial preparation to improve water quality is arranged in the background technology.Viable bacteria in the above-mentioned preparation is photosynthetic bacterium mostly, though can decompose part ammonia nitrogen and nitrate in the water of aquaculture pond pond, but be difficult to superfluous feed and the excremental larger molecular organics of aquatic products in the decomposing pool water, so above-mentioned preparation is unsuitable for being used for improving the water quality of aquaculture pond Chi Shui.And above-mentioned preparation is liquid mostly, and the life poor stability of viable bacteria is difficult to Long-term Storage, transportation, uses inconvenience.
As everyone knows, in natural soil, hay etc., a kind of subtilis (Bucillus subtilis) is arranged, this bacillus can be decomposed ammonia nitrogen and the nitrate in the water of aquaculture pond pond, can decompose the excremental larger molecular organics of superfluous feed and aquatic products again.Especially therefrom the four strain dissociants that optimize (Bucillus subtilis sp.): bacillus subtilis variant strain by using 1
#, 2
#, 3
#With 4
#, more there is vitality strong, breed fast advantage.Above-mentioned dissociant obviously is the bacterial classification source that ideal prepares compound biological water purifiers with live bacteria.The inventor application number and denomination of invention be 01112787.2 and the application for a patent for invention of ' a kind of batch culture process of bacillus subtilis variant strain by using ' in the method for cultivating above-mentioned dissociant is in batches proposed, utilize this method can prepare a large amount of above-mentioned dissociants, promptly bacillus subtilis variant strain by using 1
#, 2
#, 3
#With 4
#Wet thallus, for the batch preparations compound biological water purifiers with live bacteria is laid a good foundation.
Summary of the invention
The present invention will solve and will cultivate the bacillus subtilis variant strain by using 1 that obtains in batches
#, 2
#, 3
#With 4
#Wet thallus be prepared into the technical problem of compound biological water purifiers with live bacteria.The present invention also will solve the compound active bio water purification agent that will be prepared into and be applied to aquaculture pond, improves the water quality of Chi Shui, improves the output of aquaculture and the technical problem of quality.
The present invention solves above-mentioned first technical problem by the following technical solutions: a kind of preparation of compound biological water purifiers with live bacteria is characterized in that step:
The first step is granulated, and takes by weighing bacillus subtilis variant strain by using 1
#, 2
#, 3
#With 4
#Wet thallus, weigh 100 parts altogether, they weigh 10～30 parts, 20～45 parts, 15～30 parts and 5～30 parts separately respectively, other adds Mierocrystalline cellulose, as Microcrystalline Cellulose, methylcellulose gum, carboxymethyl cellulose, ethyl cellulose, HPMC, weighs 50～100 parts, stirred mixed 30～40 minutes, make the particle that diameter is 1～2mm, under 50～60 ℃ condition dry 25～45 minutes, water content is 2～10% granular feedstock;
Second step pressed dry sheet, took by weighing the raw material that makes of step, weighed 1 part, added the filler through sterilization, as zeolite powder, starch, carboxymethyl starch, weighed 2～7 parts, and is mixed even, is pressed into the dry plate of every heavy 3～12g, and every contains viable count 20～20,000,000,000;
The dry plate bottling that the last step makes is sealed in the encapsulation of the 3rd step, and compound biological water purifiers with live bacteria gets product.
Viable bacteria in the compound biological water purifiers with live bacteria is in dormant state, life good stability mostly.
Embodiment 1.A kind of preparation of compound biological water purifiers with live bacteria is characterized in that, step:
The first step is granulated, and takes by weighing bacillus subtilis variant strain by using 1 respectively
#, 2
#, 3
#With 4
#Wet thallus 25,40,25 and 10kg, other adds Microcrystalline Cellulose 70kg, stirs mixed 35 minutes, makes the particle that diameter is 1.5mm, under 50 ℃ condition dry 45 minutes, water content is 3～5% granular feedstock;
Second step pressed dry sheet, take by weighing the raw material 50kg that the step makes, add through autoclaved zeolite powder 150kg, evenly mixed, be pressed into diameter, thickness and weight and be respectively the circular dry plate of 2.5cm, 0.6cm and 10 ± 0.5g, every contains viable count 80～10,000,000,000;
The dry plate bottling that the last step makes is sealed in the encapsulation of the 3rd step, and compound biological water purifiers with live bacteria gets product.
The present invention solves above-mentioned second technical problem by the following technical solutions: the application of the compound biological water purifiers with live bacteria that is prepared into above-mentioned preparation method, it is characterized in that, threw in once-combined type biologic water cleaner containing living bacteria to aquaculture pond in per 7 days, each 2～5 slices/mu, every heavy 10 ± 0.5g, total impressions is 3～7 times.When dropping into this water purification agent in the aquaculture pond, they are disintegration immediately, discharges viable bacteria, and promptly bacillus subtilis variant strain by using 1
#, 2
#, 3
#With 4
#, and a large amount of breeding, the organic substance in the decomposing pool water reduces ammonia nitrogen and nitrate in the water of pond rapidly, pond water quality is purified and improves.
Embodiment 2.The application of compound biological water purifiers with live bacteria is characterized in that, cultured the pond to globe fish in per 7 days to throw in once-combined type biologic water cleaner containing living bacteria, and each 3 slices/mu, every heavy 10 ± 0.5g, total impressions is 5 times.This water purification agent is cultured the influence of pond pond water quality to globe fish: throw in back second day respectively to test tank and the water sampling test of contrast pond at every turn, the result shows: test tank water quality be improved significantly, the ammonia nitrogen of Chi Shuizhong and nitrate content obviously reduce, and see table 1 for details.
Table 1 compound biological water purifiers with live bacteria is cultured the influence of pond pond water quality to globe fish
Embodiment 3.The application of compound biological water purifiers with live bacteria is characterized in that, cultures the pond to Macrobrachium rosenbergii in per 7 days to throw in once-combined type biologic water cleaner containing living bacteria, and each injected volume is 4 slices/mu, every heavy 10 ± 0.5g, and total impressions is 5 times.This water purification agent is to the influence of aquatic products quality: threw in for the 1st time behind this water purification agent 35 days, respectively randomly draw 20 tail Macrobrachium rosenbergiis from test tank and contrast pond, compare their the long and body weight of average body, the result shows: the average body from the test tank and the Macrobrachium rosenbergii in contrast pond is long, body weight is respectively 10.5cm, 15.2g and 8.6cm, 13.1g, and the former aquatic products quality obviously is better than the latter's aquatic products quality.This water purification agent is to the influence of aquatic products output (average yield per mu): the output of the Macrobrachium rosenbergii in test tank and contrast pond is respectively 255kg and 226.75kg, and the former rate ratio latter's output increases 28.25kg, and amplification reaches 12%.
Compare with background technology, the present invention can produce following beneficial effect:
1. be bacillus subtilis variant strain by using 1 with the viable bacteria in the compound biological water purifiers with live bacteria of preparation method's preparation of the present invention
#, 2
#, 3
#With 4
#Can not only decompose ammonia nitrogen, nitrate and superfluous feed and the excremental larger molecular organics of aquatic products in the water of aquaculture pond pond, and vitality is strong and breeding is fast, after throwing in aquaculture pond, can improve the water quality of Chi Shui rapidly, help aquatic products to grow healthily and improve the output and the quality of aquaculture.
2. the compound biological water purifiers with live bacteria with preparation method's preparation of the present invention is a dry plate, and viable bacteria life good stability is suitable for Long-term Storage and transportation, and is easy to use.
Embodiment
To sum up, the related preparation method of the present invention is particularly suitable for being used for and will cultivates the bacillus subtilis variant strain by using 1 that obtains in batches
#, 2
#, 3
#With 4
#Wet thallus be prepared into compound biological water purifiers with live bacteria, this water purification agent is particularly suitable for being invested in aquaculture pond, to improve pond water quality, to guarantee aquatic products growing and improve the output and the quality of aquatic products healthily.As for embodiment, above elaborating in ' summary of the invention ', just no longer repeat here.
Claims (5)
1. the preparation of a compound biological water purifiers with live bacteria is characterized in that, step:
The first step is granulated, and takes by weighing bacillus subtilis variant strain by using 1
#, 2
#, 3
#With 4
#Wet thallus, weigh 100 parts altogether, they weigh 10～30 parts, 20～45 parts, 15～30 parts and 5～30 parts separately respectively, other adds Mierocrystalline cellulose, as Microcrystalline Cellulose, methylcellulose gum, carboxymethyl cellulose, ethyl cellulose, HPMC, weighs 50～100 parts, stirred mixed 30～40 minutes, make the particle that diameter is 1～2mm, under 50～60 ℃ condition dry 25～45 minutes, water content is 2～10% granular feedstock;
Second step pressed dry sheet, took by weighing the raw material that makes of step, weighed 1 part, added the filler through sterilization, as zeolite powder, starch, carboxymethyl starch, weighed 2～7 parts, and is mixed even, is pressed into the dry plate of every heavy 3～12g, and every contains viable count 20～20,000,000,000;
The dry plate bottling that the last step makes is sealed in the encapsulation of the 3rd step, and compound biological water purifiers with live bacteria gets product.
2. the preparation of compound biological water purifiers with live bacteria according to claim 1 is characterized in that, step:
The first step is granulated, and takes by weighing bacillus subtilis variant strain by using 1 respectively
#, 2
#, 3
#With 4
#Wet thallus 25,40,25 and 10kg, other adds Microcrystalline Cellulose 70kg, stirs mixed 35 minutes, makes the particle that diameter is 1.5mm, under 50 ℃ condition dry 45 minutes, water content is 3～5% granular feedstock;
Second step pressed dry sheet, take by weighing the raw material 50kg that the step makes, add through autoclaved zeolite powder 150kg, evenly mixed, be pressed into diameter, thickness and weight and be respectively the circular dry plate of 2.5cm, 0.6cm and 10 ± 0.5g, every contains viable count 80～10,000,000,000;
The dry plate bottling that the last step makes is sealed in the encapsulation of the 3rd step, and compound biological water purifiers with live bacteria gets product.
3. the application of the compound biological water purifiers with live bacteria made from claim 1 or 2 described preparation methods, it is characterized in that, threw in once-combined type biologic water cleaner containing living bacteria, each 2～5 slices/mu to aquaculture pond in per 7 days, every heavy 10 ± 0.5g, total impressions is 3～7 times.
4. the application of compound biological water purifiers with live bacteria according to claim 3 is characterized in that, cultured the pond to globe fish in per 7 days to throw in once-combined type biologic water cleaner containing living bacteria, and each 3 slices/mu, every heavy 10 ± 0.5g, total impressions is 5 times.
5. the application of compound biological water purifiers with live bacteria according to claim 3, it is characterized in that cultured the pond to Macrobrachium rosenbergii in per 7 days to throw in once-combined type biologic water cleaner containing living bacteria, each injected volume is 4 slices/mu, every heavy 10 ± 0.5g, total impressions is 5 times.
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| CN 01113387 CN1396123A (en) | 2001-07-12 | 2001-07-12 | Preparing process and application of composite biologic water cleaner containing living bacteria |
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| CN 01113387 CN1396123A (en) | 2001-07-12 | 2001-07-12 | Preparing process and application of composite biologic water cleaner containing living bacteria |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101333499B (en) * | 2008-05-13 | 2010-06-09 | 昆山工研院华科生物高分子材料研究所有限公司 | Complex active bacterial biological water purifying a gent and method for preparing same |
| CN101555088B (en) * | 2009-05-15 | 2011-08-24 | 李华林 | Method for treating black-odor rivers and lakes with microbiological preparations |
| CN102329009A (en) * | 2011-08-26 | 2012-01-25 | 湖北大悟科亮环保科技有限公司 | Production method of biological water purifying agent |
| CN102864137A (en) * | 2012-09-26 | 2013-01-09 | 中国海洋大学 | Microbial particle preparation for aquacultural water quality purification and preparation method |
| CN103011425A (en) * | 2012-12-27 | 2013-04-03 | 上海泓宝绿色水产科技发展有限公司 | Granular composite bottom treatment bacterium and preparation method thereof |
| CN103058385A (en) * | 2011-10-20 | 2013-04-24 | 中国水产科学研究院渔业机械仪器研究所 | Process for treatment of high density aquaculture sewage |
| CN108793427A (en) * | 2018-06-21 | 2018-11-13 | 湖南双晟科技信息咨询有限公司 | A kind of domestic sewage treating compound and preparation method thereof |
-
2001
- 2001-07-12 CN CN 01113387 patent/CN1396123A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101333499B (en) * | 2008-05-13 | 2010-06-09 | 昆山工研院华科生物高分子材料研究所有限公司 | Complex active bacterial biological water purifying a gent and method for preparing same |
| CN101555088B (en) * | 2009-05-15 | 2011-08-24 | 李华林 | Method for treating black-odor rivers and lakes with microbiological preparations |
| CN102329009A (en) * | 2011-08-26 | 2012-01-25 | 湖北大悟科亮环保科技有限公司 | Production method of biological water purifying agent |
| CN103058385A (en) * | 2011-10-20 | 2013-04-24 | 中国水产科学研究院渔业机械仪器研究所 | Process for treatment of high density aquaculture sewage |
| CN103058385B (en) * | 2011-10-20 | 2014-05-21 | 中国水产科学研究院渔业机械仪器研究所 | Process for treatment of high density aquaculture sewage |
| CN102864137A (en) * | 2012-09-26 | 2013-01-09 | 中国海洋大学 | Microbial particle preparation for aquacultural water quality purification and preparation method |
| CN103011425A (en) * | 2012-12-27 | 2013-04-03 | 上海泓宝绿色水产科技发展有限公司 | Granular composite bottom treatment bacterium and preparation method thereof |
| CN103011425B (en) * | 2012-12-27 | 2014-05-28 | 上海泓宝绿色水产科技发展有限公司 | Granular composite bottom treatment bacterium and preparation method thereof |
| CN108793427A (en) * | 2018-06-21 | 2018-11-13 | 湖南双晟科技信息咨询有限公司 | A kind of domestic sewage treating compound and preparation method thereof |
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