CN1395840A - Cumin bactericide and preparation method thereof - Google Patents
Cumin bactericide and preparation method thereof Download PDFInfo
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- CN1395840A CN1395840A CN 02139349 CN02139349A CN1395840A CN 1395840 A CN1395840 A CN 1395840A CN 02139349 CN02139349 CN 02139349 CN 02139349 A CN02139349 A CN 02139349A CN 1395840 A CN1395840 A CN 1395840A
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- cumin
- extract
- bactericide
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- missible oil
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- 235000007129 Cuminum cyminum Nutrition 0.000 title claims abstract description 79
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 31
- 239000003899 bactericide agent Substances 0.000 title claims abstract description 20
- 238000002360 preparation method Methods 0.000 title claims description 9
- 244000304337 Cuminum cyminum Species 0.000 title claims description 3
- 241000510672 Cuminum Species 0.000 claims abstract description 78
- 238000000034 method Methods 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 6
- 230000001954 sterilising effect Effects 0.000 claims description 16
- 238000004659 sterilization and disinfection Methods 0.000 claims description 16
- 239000004530 micro-emulsion Substances 0.000 claims description 13
- 239000002904 solvent Substances 0.000 claims description 9
- 239000002671 adjuvant Substances 0.000 claims description 8
- 239000002917 insecticide Substances 0.000 claims description 8
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 238000000605 extraction Methods 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 2
- 239000012153 distilled water Substances 0.000 claims description 2
- 239000000975 dye Substances 0.000 claims description 2
- 239000012467 final product Substances 0.000 claims description 2
- 235000012054 meals Nutrition 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- JBTWLSYIZRCDFO-UHFFFAOYSA-N ethyl methyl carbonate Chemical compound CCOC(=O)OC JBTWLSYIZRCDFO-UHFFFAOYSA-N 0.000 claims 3
- 239000000575 pesticide Substances 0.000 abstract description 8
- 241000196324 Embryophyta Species 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 5
- 150000001875 compounds Chemical class 0.000 abstract description 3
- 125000004122 cyclic group Chemical group 0.000 abstract description 2
- 206010059866 Drug resistance Diseases 0.000 abstract 2
- 244000052616 bacterial pathogen Species 0.000 abstract 2
- 239000000126 substance Substances 0.000 abstract 2
- 244000000005 bacterial plant pathogen Species 0.000 abstract 1
- 230000007774 longterm Effects 0.000 abstract 1
- 230000004060 metabolic process Effects 0.000 abstract 1
- 239000003921 oil Substances 0.000 description 18
- 244000052769 pathogen Species 0.000 description 7
- 241000123650 Botrytis cinerea Species 0.000 description 6
- 230000001717 pathogenic effect Effects 0.000 description 6
- 241000223195 Fusarium graminearum Species 0.000 description 5
- 241000233616 Phytophthora capsici Species 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 235000013399 edible fruits Nutrition 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 241000193738 Bacillus anthracis Species 0.000 description 4
- 241000332749 Setosphaeria turcica Species 0.000 description 4
- -1 acetate ester Chemical class 0.000 description 4
- AOGQPLXWSUTHQB-UHFFFAOYSA-N hexyl acetate Chemical compound CCCCCCOC(C)=O AOGQPLXWSUTHQB-UHFFFAOYSA-N 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000002401 inhibitory effect Effects 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- RNMDNPCBIKJCQP-UHFFFAOYSA-N 5-nonyl-7-oxabicyclo[4.1.0]hepta-1,3,5-trien-2-ol Chemical compound C(CCCCCCCC)C1=C2C(=C(C=C1)O)O2 RNMDNPCBIKJCQP-UHFFFAOYSA-N 0.000 description 2
- 241000221785 Erysiphales Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 239000003905 agrochemical Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000686 essence Substances 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000003449 preventive effect Effects 0.000 description 2
- 238000010992 reflux Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 230000001018 virulence Effects 0.000 description 2
- 206010000087 Abdominal pain upper Diseases 0.000 description 1
- 241000208306 Apium Species 0.000 description 1
- 241000228197 Aspergillus flavus Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241001480060 Blumeria Species 0.000 description 1
- 241000306559 Exserohilum Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241000233614 Phytophthora Species 0.000 description 1
- 241000116309 Prorates boydi Species 0.000 description 1
- GWGMICQFGXMPAU-UHFFFAOYSA-N S=[Ca].OC1=CC=CC=C1 Chemical compound S=[Ca].OC1=CC=CC=C1 GWGMICQFGXMPAU-UHFFFAOYSA-N 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 235000013409 condiments Nutrition 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 206010016766 flatulence Diseases 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 230000002073 mitogenetic effect Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000979 retarding effect Effects 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 230000004763 spore germination Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a bactericide prepared by using plant cumin as raw material and a method thereof, wherein roots, stems, leaves and seeds of cumin (cumin L.) are used as raw materials. The cumin bactericide is safe to the environment, active compounds in the cumin bactericide are naturally-existing substances, are derived from nature, and form an inherent way participating in energy and substance cyclic metabolism in the long-term evolution process, so that the cumin bactericide is applied to the environment or plants, has no residue, and cannot cause biological enrichment; is relatively safe for non-target organisms; the action mechanism of the pesticide on plant pathogenic bacteria is different from that of the conventional pesticide, and after the pesticide is used, the pathogenic bacteria are not easy to generate drug resistance to the pesticide, and the pesticide also has a delay effect on the drug resistance of the pathogenic bacteria to the conventional bactericide.
Description
One. affiliated field
The invention belongs to the agriculture and forestry field, be specifically related to agricultural chemicals preparation and manufacture field, particularly a kind ofly utilize that plant---cumin is bactericide of feedstock production and preparation method thereof.
Two. background technology
Cumin (Cuminum cyminum L.) is 2 years these plants of sward of Umbelliferae cumin apium life in 1 year, and Xinjiang region and Hexi Corridor, Gansu are mainly originated in China.Plant height 20 ~ 40cm, the smooth nothing hair of complete stool (except that fruit); Blade 3 goes out or 2 times winglike compound leaves, totally cleaves, and it is narrow linear that sliver is returned at the end, long 1.5 ~ 5.0cm, wide 0.3 ~ 0.5mm; Universal umbel is the dichotomy branch more, umbel diameter 2 ~ 3cm, and umbellule has 7 flowers usually, petal pink or white, Long Circle, the little tab that invagination is arranged that lacks in top, flower strain base portion is coniform; Style is short, fork, column cap head; Mitogenetic fruit Long Circle, two ends are long and narrow, long 6mm, wide 1.5mm, the white bristle of close quilt, fruit thousand kernel weight 2 ~ 3g.The cumin happiness weather that cools, more drought-enduring, adaptability is strong, soil is required not strict, generally with sandy soil earth for well.
Xinjiang of China is the main place of production of domestic cumin, and masses of all nationalities such as the local Uygur nationality, the Hui ethnic group, the diligent family of Ke Erke, Kazak make condiment with its fruit pulverize, especially are usually used in roasting in the meat.Its fruit also can be used as medicine, diseases such as treatment indigestion and stomach cold stomachache; Can increase pregnant woman's milk, reduce and feel sick; Also can be used as application, alleviate patient's swelling.Cumin also is a kind of important batching in the Uighurs medicinal herb tea of often drinking.In the west, be mainly used in the animal doctor pharmaceutically, as getting rid of the Flatulence agent.From the seed of cumin, can extract ziran oil and (claim cymin oil again, Cumin oil), it is confirmed by World Association for Sexuality organisations and institutions such as FEMA, FDA as a kind of natural plant pefume, can be used for daily essences such as food, tobacco essence and part cosmetics in a large number.In addition, report in many articles that ziran oil has the effect of various disease funguses of opposing and bacterium.Atta-ur-Rahman and partner thereof reported once that ziran oil had certain bacteriostasis to P.boydi and A.flavus.In addition, its essential oil also is commonly used for disinfectant, preservative uses.And cumin does not also have comprehensive and systematic report to the inhibitory action of phytopathogen.
Three. summary of the invention
According to some fundamental characteristics of above-mentioned cumin, the object of the present invention is to provide a kind of bactericide and method thereof of utilizing cumin for feedstock production.
To achieve these goals; the applicant is by suppressing the further investigation of various phytopathogen effects to cumin; found the hexyl acetate extract of cumin complete stool; to fusarium graminearum (Gibberella zeae); Exserohilum turcicum (Exserohilum turcicurn); Phytophthora capsici germ (Phytophthora capsicileaonian); apple anthrax bacteria (Goloetotrichum gloeosphorivides); botrytis cinerea (Botrytis cinerea) and wheat powdery mildew phytopathogens such as (Blumeria graminisr) show higher inhibitory action under isolated condition; under condition of living body, crop there is good protective effect.From the characteristic of cumin as can be known, it is nontoxic, pollution-free to people, animal, environment, meets the direction of agricultural chemicals development, is expected to be developed to a kind of non-harmful botanical fungicide.
The technical solution used in the present invention is: it is raw material that this cumin bactericide adopts root, stem, leaf, seed with cumin (Cuminum cyminumL.), be characterized in, with above-mentioned raw materials hexyl acetate solvent extraction, extract through concentrate extract, extract is mixed with cumin sterilization missible oil or microemulsion with solvent, insecticides adjuvant again.
The each component percentage by weight of cumin bactericide missible oil is:
Cumin extract 10%～70%
Solvent 20%～50%
Insecticides adjuvant 10%～20%
The each component percentage by weight of cumin bactericidal microemulsion is:
Cumin extract 10%～30%
Solvent 10%～20%
Water 30%～50%
Insecticides adjuvant 10%～20%
The preparation method of above-mentioned cumin bactericide is:
A. cumin root, stem, leaf and the seed of drying are pulverized, crossed 40 mesh sieves;
B. with 4 times to the mince acetate ester of weight of cumin powder, under refluxad extract, extract 70～80 ℃ of temperature, extraction time 8h;
C. use the method for vacuum concentration, with the cumin extract be concentrated into the cumin raw meal mince etc. heavy, the cumin extract;
D. in reactor, add each composition successively by sterilization missible oil proportional quantity, stir get final product the diligent sterilization missible oil that dyes;
E. earlier with the cumin extract, insecticides adjuvant is dissolved in the solvent, at 40 ℃～50 ℃, under the high-speed stirred situation gained solvent is added dropwise in the distilled water, and constant temperature stirs 1h, promptly gets the cumin bactericidal microemulsion.
Cumin bactericide of the present invention is compared with bactericide commonly used now, and following characteristics are arranged:
1. the reactive compound in the environmentally safe cumin bactericide is naturally occurring material, derive from nature, in the evolution of long period of time process, formed its intrinsic participation energy and material cyclic metabolic pathway, so be applied in the environment or on the plant, noresidue can not cause biological concentration;
2. to non-comparatively safe target organisms;
3. the mechanism of action to phytopathogen is different from conventional pesticide, uses the back pathogen to be difficult for it is developed immunity to drugs, and pathogen is also had retarding action to the pesticide resistance of conventional sterilization agent.
Four, embodiment
For a more clear understanding of the present invention and the drug effect of medicament of the present invention, below the following embodiment that finishes according to technical solution of the present invention that provides by the inventor the present invention is described in further detail, these only are the present invention embodiment preferably, but are not limited to these embodiment.Embodiment 1: the preparation of cumin extract
Cumin root, stem, leaf and the seed of drying are pulverized, crossed 40 mesh sieves.Take by weighing cumin powder and mince 100kg in the refluxing extraction still, add 400kg acetate ester solution again, under 70 ℃～80 ℃ temperature, refluxing extraction 8h, filter extract.Extract is concentrated into 100kg, promptly gets the cumin extract.Embodiment 2: the preparation of cumin sterilization missible oil
Take by weighing cumin extract 50kg,, under agitation add polyoxyethylene nonylphenol ether 9kg and detergent alkylate sulphur phenol calcium 1kg, stir, promptly get 100kg cumin sterilization missible oil with methyl alcohol 40kg dissolving.Embodiment 3: the preparation of cumin bactericidal microemulsion
Take by weighing cumin extract 30kg and 15kg polyoxyethylene nonylphenol ether phosphate, be dissolved in the 15kg ethanol, above solution is heated to 43 ℃ in homogenizer, drip deionized water 40kg under the high-speed stirred; Dropwise, stirred 45 minutes again, cooling promptly gets the cumin bactericidal microemulsion.Experimental example 1: measure cumin acetate the ester extract to the inhibition activity of phytopathogen
This experiment is under isolated condition, and the ester extract is to the inhibition activity of phytopathogen to have measured cumin acetate, and experimental technique and experimental result are as follows: 1. for the examination bacterial classification
Fusarium graminearum (Gibberella zeae), Exserohilum turcicum (Exserohilumturcicurn), Phytophthora capsici germ (Phytophthora capsici leaonian), apple anthrax bacteria (Goloetotrichum gloeosphorivides) and botrytis cinerea (Botrytis cinerea) provide by plant pathology research institute of Xibei Univ. of Agricultural ﹠ Forest Science ﹠ Technology.2. test method
Adopt mycelial growth rate method and spore germination method to the biological activity determination that exsomatizes for the examination pathogen above-mentioned cumin sterilization missible oil, calculate inhibiting rate by following formula:
3. result of the test
Measured cumin sterilization missible oil to virulence with growth rate method, thereby obtained the cumin extract, the results are shown in Table 1 virulence for the examination pathogen for the examination pathogen.
Table 1 cumin extract is to the toxicity test result of pathogen
Experimental example 2: the field control effectiveness test of cumin sterilization missible oil and cumin bactericidal microemulsion
| For the examination bacterial classification | The LD-P line | ???EC 50 | ??EC 50Standard error (* 10 2) | EC 5095% confidence limit | Chi-square value |
| ????????(y＝) | ???(g/L) | ????(Sx 50) | ????(g/L) | ????x 2 | |
| Fusarium graminearum apple anthrax bacteria botrytis cinerea Exserohilum turcicum Phytophthora capsici germ | ? ???2.0804+2.9060x ???3.3521+1.3485x ???3.1681+1.9058x ???1.8333+2.7488x ???2.4214+3.2735x | ??10.1078 ??16.6725 ??9.1446 ??14.1914 ??6.1335 | ????3.9906 ????9.6515 ????5.1350 ????6.4550 ????6.4520 | ????8.4348～ ????12.1126 ????9.7912～ ????28.3901 ????7.1305～ ????11.7275 ????10.1451～ ????19.8517 ????0.0049～ ????0.00771 | ????1.97 ????0.89 ????0.96 ????0.05 ????4.55 |
(medicine inspecting institute of the Ministry of Agriculture gives birth to and surveys the chamber volume to adopt " pesticide field efficacy medicine test criterion ", China Standard Press publishes, 1993.10) in the method for defined, field efficacy to cumin sterilization missible oil and cumin bactericidal microemulsion is tested, diluted concentration is 500 times and 1000 times, and result of the test sees Table 2.
The field efficacy of table 2 cumin sterilization missible oil and cumin bactericidal microemulsion
| For the examination bacterial classification | Cumin sterilization missible oil (preventive effect %) | Cumin bactericidal microemulsion (preventive effect %) | ||
| ????500x | ????1000x | ????500x | ????1000x | |
| Wheat powdery mildew | ????88.5 | ????81.1 | ????83.4 | ????74.2 |
| Apple anthrax bacteria | ????68.9 | ????55.6 | ????65.2 | ????51.3 |
| Botrytis cinerea | ????80.7 | ????72.1 | ????79.0 | ????68.5 |
| Exserohilum turcicum | ????79.3 | ????60.2 | ????76.5 | ????58.6 |
| The Phytophthora capsici germ | ????89.5 | ????75.3 | ????88.6 | ????76.2 |
Field control effectiveness test by table 2 shows: cumin sterilization missible oil and cumin bactericidal microemulsion all have higher control efficiency to five plant species pathogens for examination when diluted concentration is 500 times.
Claims (4)
1. cumin bactericide, adopting root, stem, leaf, the seed of cumin (Cuminum cyminum L.) is raw material, it is characterized in that, above-mentioned raw materials is extracted with acetate ester solvent, extract through concentrate extract, extract is mixed with cumin sterilization missible oil or microemulsion with solvent, insecticides adjuvant again.
2. cumin bactericide as claimed in claim 1 is characterized in that, the each component percentage by weight of this bactericide missible oil is:
Cumin extract 10%～70%
Solvent 20%～50%
Insecticides adjuvant 10%～20%
3. cumin bactericide as claimed in claim 1 is characterized in that, the each component percentage by weight of this bactericidal microemulsion is:
Cumin extract 10%～30%
Solvent 10%～20%
Water 30%～50%
Insecticides adjuvant 10%～20%
4. a preparation method who realizes the described cumin bactericide of claim 1 is characterized in that, carries out according to the following steps:
A. cumin root, stem, leaf and the seed of drying are pulverized, crossed 40 mesh sieves;
B. with 4 times to the mince acetate ester solvent of weight of cumin powder, under refluxad extract, extract 70～80 ℃ of temperature, extraction time 8h;
C. use the method for vacuum concentration, with the cumin extract be concentrated into the cumin raw meal mince etc. heavy, the cumin extract;
D. in reactor, add each composition successively by sterilization missible oil proportional quantity, stir get final product the diligent sterilization missible oil that dyes;
E. earlier with the cumin extract, insecticides adjuvant is dissolved in acetate in the ester solvent, under 40 ℃～50 ℃, high-speed stirred situation the gained solvent is added in the distilled water, and constant temperature stirs 1h, promptly gets the cumin bactericidal microemulsion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021393494A CN1194617C (en) | 2002-08-13 | 2002-08-13 | Cumin bactericide and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021393494A CN1194617C (en) | 2002-08-13 | 2002-08-13 | Cumin bactericide and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1395840A true CN1395840A (en) | 2003-02-12 |
| CN1194617C CN1194617C (en) | 2005-03-30 |
Family
ID=4750042
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021393494A Expired - Fee Related CN1194617C (en) | 2002-08-13 | 2002-08-13 | Cumin bactericide and preparation method thereof |
Country Status (1)
| Country | Link |
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| CN (1) | CN1194617C (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101843243A (en) * | 2010-05-28 | 2010-09-29 | 杨凌农科大无公害农药研究服务中心 | Plant origin sterilizing water-based preparation containing cumic acid and preparation method thereof |
| CN103004979A (en) * | 2012-12-11 | 2013-04-03 | 甘肃农业大学 | Preparation method for natural insect repellent |
| CN103081986A (en) * | 2013-02-28 | 2013-05-08 | 甘肃省敦煌种业股份有限公司玉门市种子公司 | Using method of natural preservative |
| CN103609674A (en) * | 2013-12-02 | 2014-03-05 | 甘肃省敦煌种业股份有限公司 | Method for preventing dry rot of potato tubers |
| CN103651601A (en) * | 2013-12-27 | 2014-03-26 | 成都新朝阳作物科学有限公司 | Pesticide composition containing cumin and common origanum extracts, and preparation method and application thereof |
| CN107646573A (en) * | 2017-09-08 | 2018-02-02 | 广西深禾丰农业科技发展有限公司 | A kind of biological control method of powdery mildew of strawberry |
| CN108004022A (en) * | 2017-12-12 | 2018-05-08 | 湖北中烟工业有限责任公司 | Preparation method for the Cuminum cyminum extract of quick-fried pearl base oil |
-
2002
- 2002-08-13 CN CNB021393494A patent/CN1194617C/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101843243A (en) * | 2010-05-28 | 2010-09-29 | 杨凌农科大无公害农药研究服务中心 | Plant origin sterilizing water-based preparation containing cumic acid and preparation method thereof |
| CN101843243B (en) * | 2010-05-28 | 2014-04-16 | 杨凌农科大无公害农药研究服务中心 | Plant origin sterilizing water-based preparation containing cumic acid and preparation method thereof |
| CN103004979A (en) * | 2012-12-11 | 2013-04-03 | 甘肃农业大学 | Preparation method for natural insect repellent |
| CN103081986A (en) * | 2013-02-28 | 2013-05-08 | 甘肃省敦煌种业股份有限公司玉门市种子公司 | Using method of natural preservative |
| CN103081986B (en) * | 2013-02-28 | 2014-12-03 | 甘肃省敦煌种业股份有限公司玉门市种子公司 | Using method of natural preservative |
| CN103609674A (en) * | 2013-12-02 | 2014-03-05 | 甘肃省敦煌种业股份有限公司 | Method for preventing dry rot of potato tubers |
| CN103651601A (en) * | 2013-12-27 | 2014-03-26 | 成都新朝阳作物科学有限公司 | Pesticide composition containing cumin and common origanum extracts, and preparation method and application thereof |
| CN103651601B (en) * | 2013-12-27 | 2015-10-28 | 成都新朝阳作物科学有限公司 | Containing the composition pesticide and its preparation method and application of cumin and wild marjoram extract |
| CN107646573A (en) * | 2017-09-08 | 2018-02-02 | 广西深禾丰农业科技发展有限公司 | A kind of biological control method of powdery mildew of strawberry |
| CN108004022A (en) * | 2017-12-12 | 2018-05-08 | 湖北中烟工业有限责任公司 | Preparation method for the Cuminum cyminum extract of quick-fried pearl base oil |
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| Publication number | Publication date |
|---|---|
| CN1194617C (en) | 2005-03-30 |
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