CN1388252A - Gene cluster for using hydrophanous streptomycete 17997 in synthesizing geldanamycin biologically - Google Patents
Gene cluster for using hydrophanous streptomycete 17997 in synthesizing geldanamycin biologically Download PDFInfo
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Abstract
The present invention relates to the cloning and prduction of antibiotic biosynthesis gene cluster from antibiotic producing bacteria and is especially that Ansha compound biosynthesis gene conserved-sequence cloning related gene is used as probe to clone and obtain geldanamycin biosynthesis gene cluster, and this lays the foundation for increasing the yield of geldanamycin and optimizing its structure.
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Technical field:
The present invention relates to being cloned from antibiotics generated bacterium and obtaining antibiotic biological synthesis gene cluster sequence, for improving antibiotic yield or transforming its structure, to obtain the more preferable antibiotics of performance.Background technology:
Water-absorption rules are isolated one plant of actinomyces from China's soil, and identified its produces geldanamycin (geldanamycin-Gdn).Geldanamycin has antibacterial, antiprotozoan and antitumor activity, it in vivo, external enwergy suppress oncogene expression product, and specifically can be combined with heat shock protein Hsp90.In recent years, because it has the features such as unique structure, mechanism of action are novel, geldanamycin receives much concern in medicine and field of biology.Geldanamycin derivant has carried out Phase I clinical trial in units such as NCI as antineoplastic.In addition, this institute is it has also been found that geldanamycin has good broad-spectrum disease resistance toxic action (Tao Peizhen etc., 1997,1998,2001).
Geldanamycin belongs to benzoquinones type ansamycins antibiotic.Known ansamycins antibiotic is to pacify Sha's chain by an aliphatic to act on by amido link bridge joint, formed by the non-neighboring near position for being connected to benzene or naphthalene chromophoric group.The synthesis of benzene or naphthalene chromophoric group is as starter unit by uncommon 3- amino -5- hydroxybenzoic acids (AHBA);It is to be formed by lower fatty acid through polyketide synthase (TypeI PKS) catalyzing and condensing that aliphatic, which pacifies Sha's chain,.The existing more research of the biosynthesis pathway of rifamycin, napthomycin, ansatrinin, ansamitocin and mitomycin C in ansamycins antibiotic, their biological synthesis gene cluster has been cloned.At present, the molecular biology background of geldanamycin biosynthesis is not also fully aware of, and associated biomolecule synthetic gene has no report.
Because ansamycins antibiotic structure is similar, thus it is speculated that their biosynthesis pathways are similar.It is therefore possible to cloned using part known sequence and obtain geldanamycin biosynthesis gene.Goal of the invention:
It is an object of the invention to utilize the molecular biology method developed now, related gene is cloned with AMSA compound biosynthesis gene conserved sequence (AHBA genes), as probe, clone and obtain geldanamycin biological synthesis gene cluster, so as to be laid the foundation to improve geldanamycin fermentation yield and Optimizing Reconstruction its structure.The content of the invention:1. cloned segment AHBA synthase genes
Utilize containing AHBA structure antibiotic rifamycinB of the biological software Vector NTIsuit6.0 programs to all logins in ncbi database, ansatrieninA, napthomycin, mitomycinC, ansamitocin etc. AHBA synthase coding sequences analysis, according to conserved regions design a pair of degenerate primers of its amino acid sequence:
Wherein R=A/G, S=G/C, M=A/C enters performing PCR reaction under LATaq enzyme effects, as a result amplifies 755bp specific bands (Fig. 1) using S.hygroscopicus17997 strains (preservation of DSMZ of this institute) genomic DNA as template.By 755bpPCR product sequencing analysis, as a result show, amino acid and the AHBA synthase in known Rifamycin Producer Amycolatopsis mediterranei of PCR primer coding;AHBA synthase conserved region very high homology in ansatrienin, napthomycin producing strains S.collinus and ansamitocin producing strains Actinosynnema pretiosum, its tetraploid rice is as follows: 2. clone and the sequence analysis of geldanamycin biosynthesis gene
755bpPCR Product Sequence analysis results illustrate the code segment gene for being cloned into AHBA synthase.Then using this PCR primer as probe, the hybridization conditions and S.hygroscopicus17997 gene libraries for selecting high rigor carry out colony hybridization and Southern molecules hybridize.Finally screening obtains positive colony from library, and hybridize through Southern molecules prove to have at BamHI 4.0kb fragments hybridization signal have 7, its BamHI restriction enzyme mapping and correlation such as Fig. 2.Large-scale sequencing has been carried out to coemid pCGBA10 where BamHI 4.0kb in shotgun cloning method.Then sequencing result is spliced, sequencing result analyzes reading frame with FramPlot programs, tetraploid rice is carried out by sequence in Blast programs and ncbi database and conserved sequence is analyzed, it was demonstrated that the gene cluster is responsible for the biosynthesis of geldanamycin.What the sequence that the current present invention is obtained had delivered Genbank has following 1-21 gene, and its situation is as follows:1.gdnM (Genbank indexed numbers are AF521894):The new gene of biosynthetic regulation of collagen is participated in, its gene size is 819bp, encodes the albumen being made up of 273 amino acid.Atggggtcat, gtggcggcgg, tccacgagcc, gcgcttgtca, tcggcgtgat, gaagattgag, , , , , 60Met, Gly, Ser, Cys, Gly, Gly, Gly, Pro, Arg, Ala, Ala, Leu, Val, Ile, Gly, Val, Met, Lys, Ile, Gluccatcgttac, gcataccggc, aacggctggc, accatggctg, ccatgtccgc, cgggccgccc, , , , , 120Pro, Ser, Leu, Arg, Ile, Pro, Ala, Thr, Ala, Gly, Thr, Met, Ala, Ala, Met, Ser, Ala, Gly, Pro, Prottccgtctcg, cacgagccgc, cgtgttcgcg, gcgatgtgcg, tcgtggtgac, ggcgctcgga, , , , , 180Phe, Arg, Leu, Ala, Arg, Ala, Ala, Val, Phe, Ala, Ala, Met, Cys, Val, Val, Val, Thr, Ala, Leu, Glycacgtgctga, tgtccggtgac, aggctgccgg, tgtgggccgt, ggccgccgc, cttcgccgga, , , , , 240His, Val, Leu, Met, Ser, Gly, Asp, Arg, Leu, Pro, Val, Trp, Ala, Val, Ala, Ala, Ala, Phe, Ala, Glyacggcggccg, gtgcgtggtgg, gttgcggggc, gggagcacgg, tgcgctggc, cgtgaccggg, , , , , 300Thr, Ala, Ala, Gly, Ala, Trp, Trp, Val, Ala, Gly, Arg, Glu, His, Gly, Ala, Leu, Ala, Val, Thr, Glygcgaccgtgg, tcgcgcaattc, ggcctccata, tggccttccg, gttcgcgga, gacggcagtc, , , , , 360Ala, Thr, Val, Val, Ala, Gln, Phe, Gly, Leu, His, Met, Ala, Phe, Arg, Phe, Ala, Glu, Thr, Ala, Valgccccagcgg, cgggaagcgcc, atgggtgacg, ggatgtccgg, tatgcgggg, cggcatgggc, , , , , 420Ala, Pro, Ala, Ala, Gly, Ser, Ala, Met, Gly, Asp, Gly, Met, Ser, Gly, Met, Arg, Gly, Gly, Met, Glygccgccccga, tgagcggcgcc, gccatgggtc, atatgcacga, tggcatggg, ccatatgcgc, , , , , 480Ala, Ala, Pro, Met, Ser, Gly, Ala, Ala, Met, Gly, His, Met, His, Asp, Gly, Met, Gly, His, Met, Argcatggcgcgg, acgcgatctcc, tccgccgcgc, cgtccatgag, tcatctgcc, gtggccctgg, , , , , 540His, Gly, Ala, Asp, Ala, Ile, Ser, Ser, Ala, Ala, Pro, Ser, Met, Ser, His, Leu, Pro, Trp, Pro, Trpgcgggtccgg, gcggggcgggc, atggccacgg, cccacctgct, cgccgccct, gatctgcggg, , , , , 600Ala, Gly, Pro, Gly, Gly, Ala, Gly, Met, Ala, Thr, Ala, His, Leu, Leu, Ala, Ala, Leu, Ile, Cys, Glyctgtggctgt, ggcgcggcgaa, cgggccgcct, tccggctcgg, ccgcgcgct, cgcggccctg, , , , , 660Leu, Trp, Leu, Trp, Arg, Gly, Glu, Arg, Ala, Ala, Phe, Arg, Leu, Gly, Arg, Ala, Leu, Ala, Ala, Leuctgttcgtcc, cgctcgtcct, cgccctgcgca, tcctgggcgc, gggtgtcac, tccgccgccc, , , , , 720Leu, Phe, Val, Pro, Leu, Val, Leu, Ala, Leu, Arg, Ile, Leu, Gly, Ala, Gly, Val, Thr, Pro, Pro, Progcatggacct, ccgcaccggc, cgtcgcccgcc, ggccgcgcgg, agtcctgct, gcggcacgtc, , , , , 780Ala, Trp, Thr, Ser, Ala, Pro, Ala, Val, Ala, Arg, Arg, Pro, Arg, Gly, Val, Leu, Leu, Arg, His, Valatcctgcgca, gagggccacc, gaggcggttc, gccatccgc, , , , , , , , , , , 819Ile, Leu, Arg, Arg, Gly, Pro, Pro, Arg, Arg, Phe, Ala, Ile, the Arg gene outcomes are shown and Streptomyces, coelicolor, A3, (, 2, ), it is assumed that memebrane protein it is homologous,But homology is relatively low.2.gdnQ (Genbank indexed numbers are AF521894):Oxide-reductase gene, is an incomplete ORF, and its gene size is 1233bp, encodes the albumen of 411 amino acid.Gtgagccagt, tcatagaaga, tgtccggaac, tcatcgatgc, ccgcatccga, cgactcaacc, , , , , 60Val, Ser, Gln, Phe, Ile, Glu, Asp, Val, Arg, Asn, Ser, Ser, Met, Pro, Ala, Ser, Asp, Asp, Ser, Thrggcgcgtgtc, cgcctgccgc, cgttgccccg, agccaggagt, cgtccatgtc, cgctggcccc, , , , , 120Gly, Ala, Cys, Pro, Pro, Ala, Ala, Val, Ala, Pro, Ser, Gln, Glu, Ser, Ser, Met, Ser, Ala, Gly, Proctcgccccgg, ccaccccatg, gcgcagggcg, acgggtgccg, ctgtacgtcc, tcgtcccgct, , , , , 180Leu, Ala, Pro, Ala, Thr, Pro, Trp, Arg, Arg, Ala, Thr, Gly, Ala, Ala, Val, Arg, Pro, Arg, Pro, Alaggccgcggcc, atcgccgtga, tcggctacta, cctgcccctg, ctcggcatcc, gcctcgccgc, , , , , 240Gly, Arg, Gly, His, Arg, Arg, Asp, Arg, Leu, Leu, Pro, Ala, Pro, Ala, Arg, His, Pro, Pro, Arg, Argcttcctggcc, gtcgacatcg, ccgcgggcga, gatcgcccgc, ggccgcgcca, ccatcccggc, , , , , 300Leu, Pro, Gly, Arg, Arg, His, Arg, Arg, Gly, Arg, Asp, Arg, Pro, Arg, Pro, Arg, His, His, Pro, Glycgcctgaccc, cggtttgcca, tctcggcaat, atgttttgcc, tcgatggagg, gtgggctcgc, , , , , 360Arg, Leu, Thr, Pro, Val, Cys, His, Leu, Gly, Asn, Met, Phe, Cys, Leu, Asp, Gly, Gly, Trp, Ala, Argatggttgccg, tggaggtggt, caaggtggcc, gatgagctga, agaacgcctt, tgacgtggtg, , , , , 420Met, Val, Ala, Val, Glu, Val, Val, Lys, Val, Ala, Asp, Glu, Leu, Lys, Asn, Ala, Phe, Asp, Val, Valgtgatcggcg, gtggcgccgc, tgggctgagc, ggggcgctgat, gctggcccg, gtcgcggcgt, , , , , 480Val, Ile, Gly, Gly, Gly, Ala, Ala, Gly, Leu, Ser, Gly, Ala, Leu, Met, Leu, Ala, Arg, Ser, Arg, Argtcggtggtgg, tgatcgacgc, gggcgccccg, cgcaacgcccc, ggcctcggc, ggtgcacgga, , , , , 540Ser, Val, Val, Val, Ile, Asp, Ala, Gly, Ala, Pro, Arg, Asn, Ala, Pro, Ala, Ser, Ala, Val, His, Glyctgctggccc, gggacgggat, ccctccggcc, gagttggtggc, ccggggccg, ggccgaggtc, , , , , 600Leu, Leu, Ala, Arg, Asp, Gly, Ile, Pro, Pro, Ala, Glu, Leu, Val, Ala, Arg, Gly, Arg, Ala, Glu, Valcgcggctacg, gcggtcaggt, ggtgtccggc, gaggtgggcgc, cgtgacccg, ggaggagtcc, , , , , 660Arg, Gly, Tyr, Gly, Gly, Gln, Val, Val, Ser, Gly, Glu, Val, Gly, Ala, Val, Thr, Arg, Glu, Glu, Sergggggcttcc, aggtggccct, gaccgatggc, cggaccgtacg, tgcgcgccg, gttgctgctg, , , , , 720Gly, Gly, Phe, Gln, Val, Ala, Leu, Thr, Asp, Gly, Arg, Thr, Val, Arg, Ala, Arg, Arg, Leu, Leu, Leugccaccgggc, tggtcgacga, gttgccggac, atcccggggct, gcggtcccg, gtggggccgg, , , , , 780Ala, Thr, Gly, Leu, Val, Asp, Glu, Leu, Pro, Asp, Ile, Pro, Gly, Leu, Arg, Ser, Arg, Trp, Gly, Arggatgtgctgc, actgtccgta, ctgccacggc, tgggaggtccg, cgaccaggc, catcggcgta, , , , , 840Asp, Val, Leu, His, Cys, Pro, Tyr, Cys, His, Gly, Trp, Glu, Val, Arg, Asp, Gln, Ala, Ile, Gly, Valctggggagcg, ggccgctgtc, ggtgcaccag, gcgctgctgtt, ccgtcagtg, gagcgacgat, , , , , 900Leu, Gly, Ser, Gly, Pro, Leu, Ser, Val, His, Gln, Ala, Leu, Leu, Phe, Arg, Gln, Trp, Ser, Asp, Aspgtcaccttct, tcccccacac, cctgccgtcg, ccgtccggcga, ggaggcgga, gcagctggcc, , , , , 960Val, Thr, Phe, Phe, Pro, His, Thr, Leu, Pro, Ser, Pro, Ser, Gly, Glu, Glu, Ala, Glu, Gln, Leu, Alagcccgtggca, tccgtgtggt, ggacggcgag, gtggcgtcct, tggagatcgt, cgaggaccgc, , , , , 1020Ala, Arg, Gly, Ile, Arg, Val, Val, Asp, Gly, Glu, Val, Ala, Ser, Leu, Glu, Ile, Val, Glu, Asp, Argctcgtcggcg, tgcggctggg, cgacggcggc, gtggtcgagcg, cgaggcgct, ggccgtcgcg, , , , , 1080Leu, Val, Gly, Val, Arg, Leu, Gly, Asp, Gly, Gly, Val, Val, Glu, Arg, Glu, Ala, Leu, Ala, Val, Alaccgcggatgg, tggcacacgc, cggtctcctg, gcggggctcgg, gctgcggcc, ggtggagcat, , , , , 1140Pro, Arg, Met, Val, Ala, His, Ala, Gly, Leu, Leu, Ala, Gly, Leu, Gly, Leu, Arg, Pro, Val, Glu, Hisccgagcggcg, gcggtgagca, catcccgtcc, gacgcgaccg, ggcgcaccga, ggtgtccggg, , , , , 1200Pro, Ser, Gly, Gly, Gly, Glu, His, Ile, Pro, Ser, Asp, Ala, Thr, Gly, Arg, Thr, Glu, Val, Ser, Glygtgtgggtcg, cgggcaatgt, caccgatctg, gcg, , , , , , , , , , , , 1233Val, Trp, Val, Ala, Gly, Asn, Val, Thr, Asp, Leu, the Ala gene outcomes are shown and Streptomyces, coelicolor oxidoreducing enzyme has higher homology,Uniformity is 42%,Similitude is 52%.S.coelicolor ------------------------------------------------------------ 1gdnQ.pro VSQFIEDVRNSSMPASDDSTGACPPAAVAPSQESSMSAGPLAPATPWRRATGAAVRPRPA 60S.coelicolor ------------------------------------------------------------ 1gdnQ.pro GRGHRRDRLLPAPARHPPRRLPGRRHRRGRDRPRPRHHPGRLTPVCHLGNMFCLDGGWAR 120 S.coelicolor PVAQVGTSAAAGALAGIEMNKMLAIADTDAALQKLSGGSPKGATG 338gdnQ.pro L-------------------------------------------- 4103.gdnS (Genbank indexed numbers are AF521894):Delta antigen gene is encoded, its size is 630bp, encodes the albumen being made up of 210 amino acid.Atgccttctg, ccacgctgcc, cgccgcaccg, ataagagccg, tgcacggcct, tgccaccgcg, , , , , 60Met, Pro, Ser, Ala, Thr, Leu, Pro, Ala, Ala, Pro, Ile, Arg, Ala, Val, His, Gly, Leu, Ala, Thr, Alagcgaacgacc, accaggtcac, cgaatgggcg, ctggccgccc, gggacggcga, ccgcgaggcg, , , , , 120Ala, Asn, Asp, His, Gln, Val, Thr, Glu, Trp, Ala, Leu, Ala, Ala, Arg, Asp, Gly, Asp, Arg, Glu, Alagtcgaccact, tcatccgcgc, cacctaccgc, gatgtgcgcc, gtttcgtgct, ccacctcagc, , , , , 180Val, Asp, His, Phe, Ile, Arg, Ala, Thr, Tyr, Arg, Asp, Val, Arg, Arg, Phe, Val, Leu, His, Leu, Sergccgatccgc, atggttgtga, ggacctcgcc, caggagacgt, atctgcgggc, gctgaccggg, , , , , 240Ala, Asp, Pro, His, Gly, Cys, Glu, Asp, Leu, Ala, Gln, Glu, Thr, Tyr, Leu, Arg, Ala, Leu, Thr, Glyctgccgcgct, tcgccggtcg, ctcatcggcc, cggacgtggc, tgctgtcgat, cgcccgccgt, , , , , 300Leu, Pro, Arg, Phe, Ala, Gly, Arg, Ser, Ser, Ala, Arg, Thr, Trp, Leu, Leu, Ser, Ile, Ala, Arg, Arggtggtcgtcg, accgctaccg, cacggccgcc, gcccgtcccc, gtacgttgga, cgcggacgac, , , , , 360Val, Val, Val, Asp, Arg, Tyr, Arg, Thr, Ala, Ala, Ala, Arg, Pro, Arg, Thr, Leu, Asp, Ala, Asp, Asptggcaggagg, cggccgaacg, ggcgcagccc, gccgggctcc, ccgggttcga, cgagggggtg, , , , , 420Trp, Gln, Glu, Ala, Ala, Glu, Arg, Ala, Gln, Pro, Ala, Gly, Leu, Pro, Gly, Phe, Asp, Glu, Gly, Valgcgctgatgg, acctgctggc, ggcgctcgcc, ccggcacgcc, gtgagatgtt, cctcctcacc, , , , , 480Ala, Leu, Met, Asp, Leu, Leu, Ala, Ala, Leu, Ala, Pro, Ala, Arg, Arg, Glu, Met, Phe, Leu, Leu, Thraaggtgctcg, gcctgccgta, cgcggacgcc, gcgaccgcga, ccggctgccc, catcggcacc, , , , , 540Lys, Val, Leu, Gly, Leu, Pro, Tyr, Ala, Asp, Ala, Ala, Thr, Ala, Thr, Gly, Cys, Pro, Ile, Gly, Thrgtacgctcgc, gcgtggcccg, cgcccgtgag, gacatctccg, cgctgctggc, cgcggccgag, , , , , 600Val, Arg, Ser, Arg, Val, Ala, Arg, Ala, Arg, Glu, Asp, Ile, Ser, Ala, Leu, Leu, Ala, Ala, Ala, Gluaaggccgcgg, gaccggtgcc, gttggtgggc, , , , , , , , , , , , , 630Lys, Ala, Ala, Gly, Pro, Val, Pro, Leu, Val, the Gly gene outcomes are shown and Streptomyces, coelicolorA3, (, 2, ), the delta antigen of SigmaE codings is homologous,Uniformity is 63%,Similitude is 73%.
The core enzyme (the β β ' of α 2) of delta antigen and RNA polymerase is combined, and produces active RNA polymerase (the β β ' δ of α 2) to recognize the promoter sequence of gene.Delta antigen plays an important role in the expression regulation of streptomyces gene.Sigma in streptomyces coelicolorE- delta antigen is relevant with oxidative pressure, the transcription and expression of its controllable thioredoxin and thioredoxin reductase operator.And thioredoxin and thioredoxin reductase are present in lactam antibiotics producing strains, it can ensure that key precursor thing alanyl-cysteinyl-figured silk fabrics amine acyl tripeptides is in reducing condition in lactam antibiotics biosynthesis, so that lactam antibiotics is normally synthesized.Geldanamycin has a lactam structure, and thioredoxin and thioredoxin reductase go back original system, the amino on geldanamycin precursor aromatic rings can be made to be in reducing condition, it is to avoid abnormal oxidation, so that amido link is normally formed.4.gdnP (Genbank indexed numbers are AF521894):Phosphorylase gene, its size is 993bp, encodes the albumen being made up of 331 amino acid.Atgaggtccg, ccggaccatc, ggcgccatcg, aacgggtcta, cgcctcggcc, cggatccacc, , , , , 60Met, Arg, Ser, Ala, Gly, Pro, Ser, Ala, Pro, Ser, Asn, Gly, Ser, Thr, Pro, Arg, Pro, Gly, Ser, Thrgggaggtccg, ggagtcggcg, tcggtgtgac, cgcaccggcc, gaccgccccg, ccgcaccggc, , , , , 120Gly, Arg, Ser, Gly, Ser, Arg, Arg, Arg, Cys, Asp, Arg, Thr, Gly, Arg, Pro, Pro, Arg, Arg, Thr, Glytccgctccca, tccgccccct, cccttccctc, gccatccctt, tcgtcgtctt, ccccttctcc, , , , , 180Ser, Ala, Pro, Ile, Arg, Pro, Leu, Pro, Ser, Leu, Ala, Ile, Pro, Phe, Val, Val, Phe, Pro, Phe, Serggaccacgtt, ctttcagacc, gcgttctttc, ggaccgcatt, cttcggaccg, cgttcttcgg, , , , , 240Gly, Pro, Arg, Ser, Phe, Arg, Pro, Arg, Ser, Phe, Gly, Pro, His, Ser, Ser, Asp, Arg, Val, Leu, Argaccgcacccg, agacccggac, cggcggtccg, ccgtaccggc, ccgcaccacg, ggagtgctca, , , , , 300Thr, Ala, Pro, Glu, Thr, Arg, Thr, Gly, Gly, Pro, Pro, Tyr, Arg, Pro, Ala, Pro, Arg, Glu, Cys, Seratgaacaccc, atccgatcag, tcatggcggc, ccgctctccg, gcgcgggtgt, cgcccccatc, , , , , 360Met, Asn, Thr, His, Pro, Ile, Ser, His, Gly, Gly, Pro, Leu, Ser, Gly, Ala, Gly, Val, Ala, Pro, Ileacctcggtgg, tcttcgacct, cgacggtgtc, ctcgtcaaca, gcttcgcggt, gatgcgcgag, , , , , 420Thr, Ser, Val, Val, Phe, Asp, Leu, Asp, Gly, Val, Leu, Val, Asn, Ser, Phe, Ala, Val, Met, Arg, Glugcgttcaccc, tcgcctacgc, cgaggtcgtc, ggcgacggtg, agccaccctt, cgaggagtac, , , , , 480Ala, Phe, Thr, Leu, Ala, Tyr, Ala, Glu, Val, Val, Gly, Asp, Gly, Glu, Pro, Pro, Phe, Glu, Glu, Tyraaccggcatc, tgggccgcta, cttccccgac, atcatgcgga, tcatgggtct, tccgctggag, , , , , 540Asn, Arg, His, Leu, Gly, Arg, Tyr, Phe, Pro, Asp, Ile, Met, Arg, Ile, Met, Gly, Leu, Pro, Leu, Gluatggagggcc, cgttcgtccg, cgagagctac, cggctcgccc, acctggtgga, gatgttcgac, , , , , 600Met, Glu, Gly, Pro, Phe, Val, Arg, Glu, Ser, Tyr, Arg, Leu, Ala, His, Leu, Val, Glu, Met, Phe, Aspggtgtgccag, agctgctgtc, ggagctgcgc, caccgcgggt, tacgactcgc, cgtggccacc, , , , , 660Gly, Val, Pro, Glu, Leu, Leu, Ser, Glu, Leu, Arg, His, Arg, Gly, Leu, Arg, Leu, Ala, Val, Ala, Thrgggaagagcg, gaccccgggc, gcgttcgctg, ctcgacaccc, tcggcatccg, tgggcagttc, , , , , 720Gly, Lys, Ser, Gly, Pro, Arg, Ala, Arg, Ser, Leu, Leu, Asp, Thr, Leu, Gly, Ile, Arg, Gly, Gln, Phecacgtggtcc, tcggctcgga, cgaggtggcc, cggcccaagc, ccgcgccgga, catcgtgctg, , , , , 780His, Val, Val, Leu, Gly, Set, Asp, Glu, Val, Ala, Arg, Pro, Lys, Pro, Ala, Pro, Asp, Ile, Val, Leuaaggcgatgg, acatgatgga, cgcggacccc, gaccggaccg, tgatggtcgg, ggacgcggtg, , , , , 840Lys, Ala, Met, Asp, Met, Met, Asp, Ala, Asp, Pro, Asp, Arg, Thr, Val, Met, Val, Gly, Asp, Ala, Valaccgacctgg, ccagcgcgcg, gggggccggg, atcaccgccg, tggccgcgat, gtggggtgag, , , , , 900Thr, Asp, Leu, Ala, Ser, Ala, Arg, Gly, Ala, Gly, Ile, Thr, Ala, Val, Ala, Ala, Met, Trp, Gly, Gluaccgacgaga, agaccctgct, cgcggcggag, cccgatgtga, tcctgcacaa, gccggcggaa, , , , , 960Thr, Asp, Glu, Lys, Thr, Leu, Leu, Ala, Ala, Glu, Pro, Asp, Val, Ile, Leu, His, Lys, Pro, Ala, Gluctgctgtcgc, tgtgccccga, ggtgacggtt, cca, 993Leu, Leu, Ser, Leu, Cys, Pro, Glu, Val, Thr, Val, the Pro gene outcomes are shown and ansatrienin, phosphatase AnsH needed for AHBA biosynthesis has high homology,Uniformity is 78%,Similitude is 88%.In addition it also shows that the phosphatase with napthomycin, rifamycin and mitomycinC has good homology, NapH:Uniformity is 69%, and similitude is 80%;RifM:Uniformity is 69%, and similitude is 80%;MitJ:Uniformity is 57%, and similitude is 68%.gdnP.pro MRSAGPSAPSNGSTPRPGSTGRSGSRRRCDRTGRPPRRTGSAPIRPLPSLAIPFVVFPFS 60AnsH.pro ------------------MTGTAG--RRVG-PGCRPCRTAAAPAP--------------- 24MitJ.pro ------------------------------------------------------------ 1RifM.pro ------------------------------------------------------------ 1napH.pro ------------------------------------------------------------ 1ansam.pr ------------------------------------------------------------ 1ansam.pr ------------------------------------------------------------ 15.gdnO (Genbank indexed numbers are AF521894):Oxide-reductase gene in AHBA biosynthesis, its size is 1131bp, and termination codon has overlapping with downstream gene initiation codon, may be in a transcript unit with downstream gene gdnP, encode the albumen being made up of 377 amino acid.Atgagcgccc, cgtccatcgg, cgagccgccg, atcaggaccg, ccgtggtggg, gctgggatgg, , , , , 60Met, Ser, Ala, Pro, Ser, Ile, Gly, Glu, Pro, Pro, Ile, Arg, Thr, Ala, Val, Val, Gly, Leu, Gly, Trpgcggcccgct, cgatctggct, gccccggctc, cgccacaacc, ccgccttcac, cgtgaccgcc, , , , , 120Ala, Ala, Arg, Ser, Ile, Trp, Leu, Pro, Arg, Leu, Arg, His, Asn, Pro, Ala, Phe, Thr, Val, Thr, Alagcggtggatc, ccgacgagcg, cggccgcgcg, gccgtcgccg, aggcggaggg, catggaccgg, , , , , 180Ala, Val, Asp, Pro, Asp, Glu, Arg, Gly, Arg, Ala, Ala, Val, Ala, Glu, Ala, Glu, Gly, Met, Asp, Argctgccggtgc, tggcggccgt, ccacgacctc, gaccccgccg, aggtggacct, ggcggtggtc, , , , , 240Leu, Pro, Val, Leu, Ala, Ala, Val, His, Asp, Leu, Asp, Pro, Ala, Glu, Val, Asp, Leu, Ala, Val, Valgcggtgccca, accatctgca, ctgtgcggtc, gccgccgagc, tgctggccaa, gggcattccg, , , , , 300Ala, Val, Pro, Asn, His, Leu, His, Cys, Ala, Val, Ala, Ala, Glu, Leu, Leu, Ala, Lys, Gly, Ile, Progtgttcctgg, agaagccggt, gtgcctgacc, tccgaggagg, ccgagcggct, ggccgaagcg, , , , , 360Val, Phe, Leu, Glu, Lys, Pro, Val, Cys, Leu, Thr, Ser, Glu, Glu, Ala, Glu, Arg, Leu, Ala, Glu, Alagagcgctcgg, gcggcgcgat, gctgctggcc, ggcagcgcgg, cgcggtaccg, cgccgatgtg, , , , , 420Glu, Arg, Ser, Gly, Gly, Ala, Met, Leu, Leu, Ala, Gly, Ser, Ala, Ala, Arg, Tyr, Arg, Ala, Asp, Valcgcgggctgt, accggatcgc, cgcccggctg, ggccatatcc, gtcatgtcga, gctcgcctgg, , , , , 480Arg, Gly, Leu, Tyr, Arg, Ile, Ala, Ala, Arg, Leu, Gly, His, Ile, Arg, His, Val, Glu, Leu, Ala, Trpgtgcggtcac, gcggcgtgcc, cgaccggggc, ggctggttca, cccagcggtc, gctcgcgggc, , , , , 540Val, Arg, Ser, Arg, Gly, Val, Pro, Asp, Arg, Gly, Gly, Trp, Phe, Thr, Gln, Arg, Ser, Leu, Ala, Glyggcggggcgc, tggtcgacct, gggctggcat, ctgttcgaca, tcgcggttcc, gctgctgggc, , , , , 600Gly, Gly, Ala, Leu, Val, Asp, Leu, Gly, Trp, His, Leu, Phe, Asp, Ile, Ala, Val, Pro, Leu, Leu, Glyaccgccgcgt, tccggcacgc, catcgggacc, gtgtcctccg, acttcatcgt, ccagcggtcc, , , , , 660Thr, Ala, Ala, Phe, Arg, His, Ala, Ile, Gly, Thr, Val, Ser, Ser, Asp, Phe, Ile, Val, Gln, Arg, Sertcccgggccg, cgtggcgcgg, cgacgacggc, gacggcccgg, cgctcctggg, cgccaacggg, , , , , 720Ser, Arg, Ala, Ala, Trp, Arg, Gly, Asp, Asp, Gly, Asp, Gly, Pro, Ala, Leu, Leu, Gly, Ala, Asn, Glyggtgccaccg, atgtcgagga, caccgcacgc, ggattcctca, tcaccgacga, cggccgttcg, , , , , 780Gly, Ala, Thr, Asp, Val, Glu, Asp, Thr, Ala, Arg, Gly, Phe, Leu, Ile, Thr, Asp, Asp, Gly, Arg, Sergtcgtgctgc, acgcgagctg, ggcctcgcac, gaggaactgg, acaccacccg, ggtgacgatc, , , , , 840Val, Val, Leu, His, Ala, Ser, Trp, Ala, Ser, His, Glu, Glu, Leu, Asp, Thr, Thr, Arg, Val, Thr, Ilegacggcagcg, cgggcagcgc, caccctgcgc, tgcaccttcg, gattcagccc, gaaccgcctc, , , , , 900Asp, Gly, Ser, Ala, Gly, Ser, Ala, Thr, Leu, Arg, Cys, Thr, Phe, Gly, Phe, Ser, Pro, Asn, Arg, Leugagaagtcca, ccctgacccg, taccgtcgac, ggtacgaccc, ggccggtggc, cgtacccacc, , , , , 960Glu, Lys, Ser, Thr, Leu, Thr, Arg, Thr, Val, Asp, Gly, Thr, Thr, Arg, Pro, Val, Ala, Val, Pro, Thrgaaccggtcg, gcaccgagta, cgaccggcag, ctcgacctgc, ttcccgcgca, actgcgcgac, , , , , 1020Glu, Pro, Val, Gly, Thr, Glu, Tyr, Asp, Arg, Gln, Leu, Asp, Leu, Leu, Pro, Ala, Gln, Leu, Arg, Aspccggccgggc, ggggccgggt, gatcgatgag, gtccgccgga, ccatcggcgc, catcgaacgg, , , , , 1080Pro, Ata, Gly, Arg, Gly, Arg, Val, Ile, Asp, Glu, Val, Arg, Arg, Thr, Ile, Gly, Ala, Ile, Glu, Arggtctacgcct, cggcccggat, ccaccgggag, gtccgggagt, cggcgtcggtg, , , , , , , , 1131Val, Tyr, Ala, Ser, Ala, Arg, Ile, His, Arg, Glu, Val, Arg, Glu, Ser, Ala, Ser, the Val gene outcomes are shown and ansatrienin, oxidoreducing enzyme needed for AHBA biosynthesis has high homology,AnsG:Uniformity is 57%, and similitude is 64%.In addition, the oxidoreducing enzyme related to the other antibiotic of structure containing AHBA AHBA synthesis also has homology, ansamitocins:Uniformity is 50%, and similitude is 59%. 6.gdnA (Genbank indexed numbers are AF521894):AHBA synthase genes, its size is 1152bp, and the gene end password has overlapping with gene gdnO downstream, and the two possible genes are in same transcript unit, encodes the albumen being made up of 384 amino acid.Gtgcgactgc, gatccgagct, gcccgcatgg, ccgcagtacg, gcgacgagga, gcgcgaggcc, , , , , 60Val, Arg, Leu, Arg, Ser, Glu, Leu, Pro, Ala, Trp, Pro, Gln, Tyr, Gly, Asp, Glu, Glu, Arg, Glu, Alactcatccggg, ctctggatca, ggggcaatgg, tggcgtatcg, ggggcggtga, ggtcgacgcc, , , , , 120Leu, Ile, Arg, Ala, Leu, Asp, Gln, Gly, Gln, Trp, Trp, Arg, Ile, Gly, Gly, Gly, Glu, Val, Asp, Alattcgaggcgg, agttcgccgc, ggcccatgga, agcgagcacg, ccctggcggt, caccaacggg, , , , , 180Phe, Glu, Ala, Glu, Phe, Ala, Ala, Ala, His, Gly, Ser, Glu, His, Ala, Leu, Ala, Val, Thr, Asn, Glyacgcatgcgc, tggagctcgc, cctcgaagtg, ctcggggtcg, gcgccgactc, cgaggtgatc, , , , , 240Thr, His, Ala, Leu, Glu, Leu, Ala, Leu, Glu, Val, Leu, Gly, Val, Gly, Ala, Asp, Ser, Glu, Val, Ilegttcccgcgt, tcaccttcat, ctcgtcctcg, caggcggctc, agcggctggg, cgcggtggcc, , , , , 300Val, Pro, Ala, Phe, Thr, Phe, Ile, Ser, Ser, Ser, Gln, Ala, Ala, Gln, Arg, Leu, Gly, Ala, Val, Alagtgcccgtgg, acgtggaccc, ggacacgtac, tgcatcgatc, cctcagcggt, cgaggcggcc, , , , , 360Val, Pro, Val, Asp, Val, Asp, Pro, Asp, Thr, Tyr, Cys, Ile, Asp, Pro, Ser, Ala, Val, Glu, Ala, Alaatcggcccga, aaacccgcgc, gatcatgccg, gtgcacatgg, cgggccagatg, tgcgacatg, , , , , 420Ile, Gly, Pro, Lys, Thr, Arg, Ala, Ile, Met, Pro, Val, His, Met, Ala, Gly, Gln, Met, Cys, Asp, Metgacgcgctgg, gcaagctgtc, cgccgactcg, ggggtgccgc, tgatccagga, cgcggcccat, , , , , 480Asp, Ala, Leu, Gly, Lys, Leu, Ser, Ala, Asp, Ser, Gly, Val, Pro, Leu, Ile, Gln, Asp, Ala, Ala, Hisgcgcacggtg, cgcggtggcg, cggtcagaag, gtcggtgagc, tgggctcggt, cgccgcgttc, , , , , 540Ala, His, Gly, Ala, Arg, Trp, Arg, Gly, Gln, Lys, Val, Gly, Glu, Leu, Gly, Ser, Val, Ala, Ala, Pheagcttccaga, acggaaagct, gatgacggcc, ggcgagggcg, gcgccgtgct, cttccccgat, , , , , 600Ser, Phe, Gln, Asn, Gly, Lys, Leu, Met, Thr, Ala, Gly, Glu, Gly, Gly, Ala, Val, Leu, Phe, Pro, Aspgccgagatgt, acgagagggg, cttcgtccgg, cacagctgtg, gacgtccgcg, caccgaccgc, , , , , 660Ala, Glu, Met, Tyr, Glu, Arg, Gly, Phe, Val, Arg, His, Ser, Cys, Gly, Arg, Pro, Arg, Thr, Asp, Argggctacttcc, accgcacctc, gggctccaac, ttccggctga, acgagttctc, cgcatcggta, , , , , 720Gly, Tyr, Phe, His, Arg, Thr, Ser, Gly, Ser, Asn, Phe, Arg, Leu, Asn, Glu, Phe, Ser, Ala, Ser, Valctgcgcgccc, aactcacccg, cctggacggc, cagatcacca, cgcgtgagca, gcgctggccg, , , , , 780Leu, Arg, Ala, Gln, Leu, Thr, Arg, Leu, Asp, Gly, Gln, Ile, Thr, Thr, Arg, Glu, Gln, Arg, Trp, Progtgctgagca, ggctgctcgc, cgagatcccc, ggtgtggtac, cgcagtcgcg, cgacgaccgc, , , , , 840Val, Leu, Ser, Arg, Leu, Leu, Ala, Glu, Ile, Pro, Gly, Val, Val, Pro, Gln, Ser, Arg, Asp, Asp, Argggtgaccgca, atccgcacta, catggcgatg, ttccgggtgc, cgggcatcac, cgaggagcgc, , , , , 900Gly, Asp, Arg, Asn, Pro, His, Tyr, Met, Ala, Met, Phe, Arg, Val, Pro, Gly, Ile, Thr, Glu, Glu, Argcgtgcgaagg, tcgtcgacac, cctcatcgag, cgcggggtgc, ccgcgttcgt, cgcgttccgc, , , , , 960Arg, Ala, Lys, Val, Val, Asp, Thr, Leu, Ile, Glu, Arg, Gly, Val, Pro, Ala, Phe, Val, Ala, Phe, Arggcggtctacc, gtacggacgc, cttctgggag, gtcgcggcgc, cggatctgac, ggtggacgaa, , , , , 1020Ala, Val, Tyr, Arg, Thr, Asp, Ala, Phe, Trp, Glu, Val, Ala, Ala, Pro, Asp, Leu, Thr, Val, Asp, Gluctcgcccgcc, gctgcccgca, ctccgaggcg, ctcacccgcg, actgcctttg, gctgcaccac, , , , , 1080Leu, Ala, Arg, Arg, Cys, Pro, His, Ser, Glu, Ala, Leu, Thr, Arg, Asp, Cys, Leu, Trp, Leu, His, Hiscgggtgttgc, tgggcagcga, ggagcagatg, cacgaagtgg, ccgccgtcgt, cgccgatgtg, , , , , 1140Arg, Val, Leu, Leu, Gly, Ser, Glu, Glu, Gln, Met, His, Glu, Val, Ala, Ala, Val, Val, Ala, Asp, Valctcgcgggcg, ca, , , , , , , , , , , , , , , , , , 1152Leu, Ala, Gly, the Ala gene outcomes, which are shown with the AHBA synthase AnsF needed for ansatrieninAHBA biosynthesis, high homology,Uniformity is 73%,Similitude is 78%.It also show has homology with the AHBA synthase of the antibiotic of other structures containing AHBA:Ansamitocins producing strains Actinosynnema pretiosum subsp.auranticum:Uniformity is 68%, and similitude is 77%;rifamycin(RifK):Uniformity is 65%, and similitude is 76%;napthomycin(NapF):Uniformity is 60%, and similitude is 70%.The amino acid size that the gene speculates is also similar to known AHBA synthase amino acid size.
The final step of gene outcome catalysis AHBA synthesis, its function is that 5- deoxidation 5- amino -3 dehydroshikimate (aminoDHS) fragrance is turned into AHBA.In addition, the gene outcome also shows that the aminopherase with a variety of sources has certain homology, for example, it in streptomycin producing strains Streptomycetesgriseus with encoding Glu:Mud crab-inose-aminopherase is homologous, and uniformity is 34%, and similitude is 48%.From homology analysis, AHBA synthase plays catalytic action except the final step formed in AHBA, and it is likely to the second function with similar aminopherase, played a role in terms of the introducing of nitrogen.7.gdnE (Genbank indexed numbers are AF521894):Amino dehydroquinic acid (amino-DHQ) dehydrase gene needed for AHBA biosynthesis, its size is 444bp, and the genetic transcription direction is with gdnA on the contrary, the albumen that coding is made up of 148 amino acid.Gtgaggtgcc, cattgagcag, gctgttgttg, gtgaacggac, cgaatctcgg, catactcggc, , , , , 60Val, Arg, Cys, Pro, Leu, Ser, Arg, Leu, Leu, Leu, Val, Asn, Gly, Pro, Asn, Leu, Gly, Ile, Leu, Glyaagcgccagc, ccgagatcta, cggcacggat, acgcttcagg, acatcgagcg, ctgggtcggg, , , , , 120Lys, Arg, Gln, Pro, Glu, Ile, Tyr, Gly, Thr, Asp, Thr, Leu, Gln, Asp, Ile, Glu, Arg, Trp, Val, Glygaagaggtcg, cggagcgcgg, ctggaaagtg, gattcctacc, agttcgatgg, cgaagcggag, , , , , 180Glu, Glu, Val, Ala, Glu, Arg, Gly, Trp, Lys, Val, Asp, Ser, Tyr, Gln, Phe, Asp, Gly, Glu, Ala, Gluatcatccaga, ccattcaggg, gaactacgac, acggtcggtg, ccatcatcaa, tccggccgcg, , , , , 240Ile, Ile, Gln, Thr, Ile, Gln, Gly, Asn, Tyr, Asp, Thr, Val, Gly, Ala, Ile, Ile, Asn, Pro, Ala, Alactcatgatgg, ccggatgggg, ccttcgggac, gcactggcga, actatccgcg, gccctggata, , , , , 300Leu, Met, Met, Ala, Gly, Trp, Gly, Leu, Arg, Asp, Ala, Leu, Ala, Asn, Tyr, Pro, Arg, Pro, Trp, Ilegaagtgcatc, tgtcgaatgt, ctgggcccgt, gagcagttcc, gccatgagtc, ggtgaccgga, , , , , 360Glu, Val, His, Leu, Ser, Asn, Val, Trp, Ala, Arg, Glu, Gln, Phe, Arg, His, Glu, Ser, Val, Thr, Glyccgctggccg, cgggtgtcat, cttcgggctc, ggcgccctgg, gctaccggct, cgccgcccgc, , , , , 420Pro, Leu, Ala, Ala, Gly, Val, Ile, Phe, Gly, Leu, Gly, Ala, Leu, Gly, Tyr, Arg, Leu, Ala, Ala, Arggccctgctcg, acaaggtgcc, ggac, , , , , , , , , , , , , , , 444Ala, Leu, Leu, Asp, Lys, Val, Pro, the Asp gene outcomes show the amino dehydroquinic acid needed for the AHBA biosynthesis with ansatrienin, (, amino-DHQ, ), dehydratase AnsE has high homology,Uniformity is 68%,Similitude is 81%.It also show has homology with the amino dehydroquinate dehydratase of the antibiotic of other structures containing AHBA:rifamycin(rifJ):Uniformity is 67%, and similitude is 78%;mitomycin C(MmcF):Uniformity is 65%, and similitude is 79%. AnsE.pro S----- 138MmcF.pro D----- 145rifJ.pro SPNGLR 174gdnE.pro --- --- 148GdnE functions are 5- deoxidation -5- amino -3- dehydroquinic acids (amino-DHQ) is removed hydrone, are changed into 5- deoxidation -5- amino -3- dehydroshikimates (amino-DHS).8.gdnK (Genbank indexed numbers are AF521894):Kinase gene needed for AHBA biosynthesis, its size is 882bp, and the genetic transcription direction is identical with gdnE, and they are likely to be at same transcript unit from the point of view of reading frame analysis, encodes the albumen being made up of 294 amino acid.Gtggcgctgc, gcctcgaaca, cgacgacctc, ggcatcagcg, aatcctcctt, ccgctggccc, , , , , 60Val, Ala, Leu, Arg, Leu, Glu, His, Asp, Asp, Leu, Gly, Ile, Ser, Glu, Ser, Ser, Phe, Arg, Trp, Progagccggacg, gtacggacgc, catgacgtcc, gcgtccggtg, gcgccacccg, cgatctggac, , , , , 120Glu, Pro, Asp, Gly, Thr, Asp, Ala, Met, Thr, Ser, Ala, Ser, Gly, Gly, Ala, Thr, Arg, Asp, Leu, Aspctgctggcgc, gccacatcag, ggagctgtgt, gcgggccgac, ccgagcggct, cacaggtgtc, , , , , 180Leu, Leu, Ala, Arg, His, Ile, Arg, Glu, Leu, Cys, Ala, Gly, Arg, Pro, Glu, Arg, Leu, Thr, Gly, Valggggtcgcga, tgcccgccac, cctcgacgcc, accggcacgg, tcaccgcctg, gcccggccgt, , , , , 240Gly, Val, Ala, Met, Pro, Ala, Thr, Leu, Asp, Ala, Thr, Gly, Thr, Val, Thr, Ala, Trp, Pro, Gly, Argcccagctggg, ccggagtgga, tctgcgcggc, gcgctgtccg, ccctcttcgg, ccacgccgag, , , , , 300Pro, Ser, Trp, Ala, Gly, Val, Asp, Leu, Arg, Gly, Ala, Leu, Ser, Ala, Leu, Phe, Gly, His, Ala, Glugtgcgctgcg, ccgacgacgg, cgatctggcc, gccctcgccg, aagcacacga, agcccgctgc, , , , , 360Val, Arg, Cys, Ala, Asp, Asp, Gly, Asp, Leu, Ala, Ala, Leu, Ala, Glu, Ala, His, Glu, Ala, Arg, Cyscccgacctgc, tctatctcgg, cgtcggcacc, gggataggcg, gtggcatcgt, gctgaacggg, , , , , 420Pro, Asp, Leu, Leu, Tyr, Leu, Gly, Val, Gly, Thr, Gly, Ile, Gly, Gly, Gly, Ile, Val, Leu, Asn, Glyaaacccgtgc, ccggtgtggg, ccgcggctcc, tgcgaagtcg, gccacctggt, cgtggaccgc, , , , , 480Lys, Pro, Val, Pro, Gly, Val, Gly, Arg, Gly, Ser, Cys, Glu, Val, Gly, His, Leu, Val, Val, Asp, Arggacggaccgc, tgtgcgactg, cggtcggcgc, ggctgcgtcc, aggcggcggc, ctcgggcccg, , , , , 540Asp, Gly, Pro, Leu, Cys, Asp, Cys, Gly, Arg, Arg, Gly, Cys, Val, Gln, Ala, Ala, Ala, Ser, Gly, Progcgaccctgc, gcagggcggc, gcggagacgg, gacgaggagg, tgaccttcac, cgcgctgcgc, , , , , 600Ala, Thr, Leu, Arg, Arg, Ala, Ala, Arg, Arg, Arg, Asp, Glu, Glu, Val, Thr, Phe, Thr, Ala, Leu, Argcaagcggtgc, gcggcggaaa, gccgtgggcg, gtggcgtcgc, tgcgggagag, cggcagggcc, , , , , 660Gln, Ala, Val, Arg, Gly, Gly, Lys, Pro, Trp, Ala, Val, Ala, Ser, Leu, Arg, Glu, Ser, Gly, Arg, Alactggccgcgg, ccgtgaccgg, cgtatgcgaa, ctgctccatc, cctcgctcgt, gctgatcggc, , , , , 720Leu, Ala, Ala, Ala, Val, Thr, Gly, Val, Cys, Glu, Leu, Leu, His, Pro, Ser, Leu, Val, Leu, Ile, Glyggagggtttg, ccgcggcgat, gccggagctg, gtggcgatgg, tggccgagcg, gacggcggag, , , , , 780Gly, Gly, Phe, Ala, Ala, Ala, Met, Pro, Glu, Leu, Val, Ala, Met, Val, Ala, Glu, Arg, Thr, Ala, Gluctggggcgcc, ccggccatcc, accgccaccg, gtccggcccg, cgcgactggg, cgggctgtcc, , , , , 840Leu, Gly, Arg, Pro, Gly, His, Pro, Pro, Pro, Pro, Val, Arg, Pro, Ala, Arg, Leu, Gly, Gly, Leu, Sertcactgcacg, gcgccgtgct, gctggccagg, ggactgccgg, ac, , , , , , , , , , 882Ser, Leu, His, Gly, Ala, Val, Leu, Leu, Ala, Arg, Gly, Leu, Pro, the Asp gene outcomes are shown and mitomycinC, kinases MitS needed for AHBA biosynthesis has high homology,Uniformity is 46%,Similitude is 51%.It also show has homology with the kinases of the antibiotic of other structures containing AHBA:rifamycin(RifN):Uniformity is 44%, and similitude is 53%;napthomycin(NapI):Uniformity is 41%, and similitude is 47%. 9.gdnT (Genbank indexed numbers are AF521894):The new gene of Unknown Function, its size is 774bp, encodes the albumen being made up of 258 amino acid.Atgctgcgcc, actacgacgc, catcggactg, ctgcgcccgg, cccatgtcga, ccccgccacc, , , , , 60Met, Leu, Arg, His, Tyr, Asp, Ala, Ile, Gly, Leu, Leu, Arg, Pro, Ala, His, Val, Asp, Pro, Ala, Thrggctaccgcc, actactcggc, cgcccagctc, agccgcctga, accgggtcat, cgcgctcaaa, , , , , 120Gly, Tyr, Arg, His, Tyr, Ser, Ala, Ala, Gln, Leu, Ser, Arg, Leu, Asn, Arg, Val, Ile, Ala, Leu, Lysgagctcggct, tcaccctcca, gcaggtgcgg, gacatcgtgg, acgagaaggt, cggcaccgag, , , , , 180Glu, Leu, Gly, Phe, Thr, Leu, Gln, Gln, Val, Arg, Asp, Ile, Val, Asp, Glu, Lys, Val, Gly, Thr, Glugagctgcgcg, gcatgctgcg, gttgcgccgg, gccgagctgg, aagccacggt, ggaagccgtg, , , , , 240Glu, Leu, Arg, Gly, Met, Leu, Arg, Leu, Arg, Arg, Ala, Glu, Leu, Glu, Ala, Thr, Val, Glu, Ala, Valgcggcacggc, tggtgcaggt, cgaggcgagg, ctccggtcga, tcgaaagcga, ggggcacatg, , , , , 300Ala, Ala, Arg, Leu, Val, Gln, Val, Glu, Ala, Arg, Leu, Arg, Ser, Ile, Glu, Ser, Glu, Gly, His, Metcccaccgacg, acgtcgtcat, caagagggtc, cccgcggtgc, gggtggcgga, gctcaccgcg, , , , , 360Pro, Thr, Asp, Asp, Val, Val, Ile, Lys, Arg, Val, Pro, Ala, Val, Arg, Val, Ala, Glu, Leu, Thr, Alaaccgccgcca, gcttcgaccc, gcaggacatc, agcccggtca, tcacacccct, ctacgaagag, , , , , 420Thr, Ala, Ala, Ser, Phe, Asp, Pro, Gln, Asp, Ile, Ser, Pro, Val, Ile, Thr, Pro, Leu, Tyr, Glu, Gluctgttccggc, ggctcgacgc, tgcgggcatc, accccgacgg, gccctggtgt, cgcatactac, , , , , 480Leu, Phe, Arg, Arg, Leu, Asp, Ala, Ala, Gly, Ile, Thr, Pro, Thr, Gly, Pro, Gly, Val, Ala, Tyr, Tyrgaggacgccc, cggaaggcgg, cggcgccatc, agtgtgcacg, ccgccgtcca, ggtgtccgcc, , , , , 540Glu, Asp, Ala, Pro, Glu, Gly, Gly, Gly, Ala, Ile, Ser, Val, His, Ala, Ala, Val, Gln, Val, Ser, Alaccgtcacggg, acggcgatga, cctccggatc, ctcgatctgc, cgcccatcga, ccacgccgcc, , , , , 600Pro, Ser, Arg, Asp, Gly, Asp, Asp, Leu, Arg, Ile, Leu, Asp, Leu, Pro, Pro, Ile, Asp, His, Ala, Alaaccatcgtcc, accgcggccc, gatggacgcc, gtggtgccca, cggcccaggc, cctggcccat, , , , , 660Thr, Ile, Val, His, Arg, Gly, Pro, Met, Asp, Ala, Val, Val, Pro, Thr, Ala, Gln, Ala, Leu, Ala, Histggattgacg, gcaacggcta, ccggtcgacc, ggctaccccc, gggagatcac, cctggagtgc, , , , , 720Trp, Ile, Asp, Gly, Asn, Gly, Tyr, Arg, Ser, Thr, Gly, Tyr, Pro, Arg, Glu, Ile, Thr, Leu, Glu, Cysccggagaacc, gtgcggaatg, ggtcacggaa, ctccagacac, cggtggtcca, ggtc, , , , , , , 774Pro, Glu, Asn, Arg, Ala, Glu, Trp, Val, Thr, Glu, Leu, Gln, Thr, Pro, Val, Val, Gln, the Val gene outcomes are shown and Streptomyces, reticuli orf1 has high homology,Uniformity is 128/172 (74%),Similitude is 140/172 (80%).orf1.pro ------------------------------------------------------------ 1gdnT.pro MLRHYDAIGLLRPAHVDPATGYRHYSAAQLSRLNRVIALKELGFTLQQVRDIVDEKVGTE 60
It also show and a kind of transcription modulator of nitrogen-fixing bacteria homologous (31%) in addition.Amino acid sequence analysis is shown, contains two domains in its N-terminal:One has helix-turn-helix structures, with the coded sequence very high homology (98.6%) with mercury resistance operon regulatory protein merR;Another and bacterial regulatory proteins Brp conserved regions very high homology (100.0%), amino acid sequence analysis is shown, contains two domains in its N-terminal:One has helix-turn-helix structures, with the coded sequence very high homology GdnT with mercury resistance operon regulatory protein: 11 FTIGDFARHGRVSVRMLRHYDAIGLLRPAHVDPATGYRHYSAAQLSRLNRVIALKELGFT 70merR: 1 YTIGEVAKLAGVSVRTLRYYERIGLLPPPIRTEG-GYRLYSDEDLERLRFIKRLKELGFS 59GdnT: 71 LQQVRDIVD 79merR: 60 LEEIKELLE 68GdnT: 14 GDFARHGRVSVRMLRHYDAIGLLRPAHVDPATGYRHY 50Brp:1 GEVAKLAGVSVETLRYYEKIGLLPPP-VRTEGGYRRY 36 the two domains belong to merR transcription regulaton factors family, and they also show that the transcription regulaton factor conserved region for transporting regulatory factor and Streptomyces coelicolor A3 (2) merR families with multiple medicine is homologous.MerR families and the reaction of regulation bacterium pressure such as toxicant, oxygen radical etc. to external world are closely related.Activity factor is combined the DNA binding structural domains that MerR albumen is guarded by its N-terminal together, the expression of albumen in multiple medicine transportation system on cell membrane is adjusted, so as to resist ambient pressure.It is indicated above the gene outcome relevant with resistance mechanism.Resistance related gene is located in the starter unit AHBA gene clusters of antibiotic, points out close to the border of biological synthesis gene cluster.10.gdnB (Genbank indexed number AF521895):Geldanamycin aliphatic pacifies Sha's chain polyketide synthase gene, and size is 2115bp, the albumen of 705 amino acid compositions of coding.Gtggctctgc, cggaggaaca, tcgggtccag, gcggcggggt, tcgggcttca, tccggcactc, , , , , 60Val, Ala, Leu, Pro, Glu, Glu, His, Arg, Val, Gln, Ala, Ala, Gly, Phe, Gly, Leu, His, Pro, Ala, Leuctcgacgcgg, ccatgcacac, catcgccttc, cacgaccgag, acgaagccga, tgcggagctc, , , , , 120Leu, Asp, Ala, Ala, Met, His, Thr, Ile, Ala, Phe, His, Asp, Arg, Asp, Glu, Ala, Asp, Ala, Glu, Leugtgctgccgt, tcgcctatcg, agaggtggcg, ttgcatgctt, cgggggcttc, ggcgctgcgg, , , , , 180Val, Leu, Pro, Phe, Ala, Tyr, Arg, Glu, Val, Ala, Leu, His, Ala, Ser, Gly, Ala, Ser, Ala, Leu, Arggtacgcgtaa, caccgtccgg, cccgaacgcc, atgaccctcg, acctggccga, tggctccggg, , , , , 240Val, Arg, Val, Thr, Pro, Ser, Gly, Pro, Asn, Ala, Met, Thr, Leu, Asp, Leu, Ala, Asp, Gly, Ser, Glygccccggttg, cctcggtggg, ctcggtggtg, tcgcgtccgg, tcggcgccga, gcacttcggc, , , , , 300Ala, Pro, Val, Ala, Ser, Val, Gly, Ser, Val, Val, Ser, Arg, Pro, Val, Gly, Ala, Glu, His, Phe, Glyacggtggcga, cggcggaccg, gatgttccgc, gtcgcatggg, aggaactgcc, gattcagccg, , , , , 360Thr, Val, Ala, Thr, Ala, Asp, Arg, Met, Phe, Arg, Val, Ala, Trp, Glu, Glu, Leu, Pro, Ile, Gln, Progacggcacga, ccgcggaacc, cgtaccggtg, gccgatgccg, aggacgtgca, ccgtctggtc, , , , , 420Asp, Gly, Thr, Thr, Ala, Glu, Pro, Val, Pro, Val, Ala, Asp, Ala, Glu, Asp, Val, His, Arg, Leu, Valacggcgccag, agacctcacc, gcccgatgtg, ctgttgctgg, acctgggcgg, tggcgtcggc, , , , , 480Thr, Ala, Pro, Glu, Thr, Ser, Pro, Pro, Asp, Val, Leu, Leu, Leu, Asp, Leu, Gly, Gly, Gly, Val, Glyggtggttcgg, ccgacgtacg, cgagctgacc, ggacgggcgt, tgcgcgttgt, acagacgtgg, , , , , 540Gly, Gly, Ser, Ala, Asp, Val, Arg, Glu, Leu, Thr, Gly, Arg, Ala, Leu, Arg, Val, Val, Gln, Thr, Trpctggaggagc, cctcgttggc, gttgagtcgg, ctggtcgtgg, tgacgcgggg, cgccgtggcc, , , , , 600Leu, Glu, Glu, Pro, Ser, Leu, Ala, Leu, Ser, Arg, Leu, Val, Val, Val, Thr, Arg, Gly, Ala, Val, Alagtccgggaga, gcgatccggt, cgatccggcg, atggcagcgg, tatgggggct, gatgggatcc, , , , , 660Val, Arg, Glu, Ser, Asp, Pro, Val, Asp, Pro, Ala, Met, Ala, Ala, Val, Trp, Gly, Leu, Met, Gly, Sergcacaagcgg, agaaccccgg, gcgcatcctc, ctcctcgaca, tcgatcaagg, gacgataccg, , , , , 720Ala, Gln, Ala, Glu, Asn, Pro, Gly, Arg, Ile, Leu, Leu, Leu, Asp, Ile, Asp, Gln, Gly, Thr, Ile, Proaccccgctac, tgcccgcact, gctcgtcggt, gaccagcacc, aactggccct, acgcgacacc, , , , , 780Thr, Pro, Leu, Leu, Pro, Ala, Leu, Leu, Val, Gly, Asp, Gln, His, Gln, Leu, Ala, Leu, Arg, Asp, Thracctgcttca, cccgccacct, catccgtgtg, ctggatgcgc, cgcagtccgg, tccgggtggt, , , , , 840Thr, Cys, Phe, Thr, Arg, His, Leu, Ile, Arg, Val, Leu, Asp, Ala, Pro, Gln, Ser, Gly, Pro, Gly, Glyttggaggacg, tgggtgggac, ggtactggtg, acgggtggga, cgggggcgtt, gggtgcggtg, , , , , 900Leu, Glu, Asp, Val, Gly, Gly, Thr, Val, Leu, Val, Thr, Gly, Gly, Thr, Gly, Ala, Leu, Gly, Ala, Valgtggcacggc, atctggtggc, ggtgcacggg, atgcggagtg, tggtgttggc, gagccggaat, , , , , 960Val, Ala, Arg, His, Leu, Val, Ala, Val, His, Gly, Met, Arg, Ser, Val, Val, Leu, Ala, Ser, Arg, Asngggcttgagg, cacccggcgc, cgccgagttg, gaggcggagc, tggtgaaggc, gggtgcgcgc, , , , , 1020Gly, Leu, Glu, Ala, Pro, Gly, Ala, Ala, Glu, Leu, Glu, Ala, Glu, Leu, Val, Lys, Ala, Gly, Ala, Arggtacgcatcg, tcgcgtgtga, tgtggcggac, cgggacgcgg, tggccgggct, gctggacgcc, , , , , 1080Val, Arg, Ile, Val, Ala, Cys, Asp, Val, Ala, Asp, Arg, Asp, Ala, Val, Ala, Gly, Leu, Leu, Asp, Alagtcccggcag, acgctccgtt, gtcggcggtg, gtgcatacgg, ccggtgttct, ggatgacggt, , , , , 1140Val, Pro, Ala, Asp, Ala, Pro, Leu, Ser, Ala, Val, Val, His, Thr, Ala, Gly, Val, Leu, Asp, Asp, Glygtgctgacgg, cgttgacccc, ggaacgtatg, gacgcggtgc, tccggccgaa, ggtggacggc, , , , , 1200Val, Leu, Thr, Ala, Leu, Thr, Pro, Glu, Arg, Met, Asp, Ala, Val, Leu, Arg, Pro, Lys, Val, Asp, Glygcactccatc, tccacgagct, gacccggcac, ctgggcctgt, ccgccttcgt, cctgttctcc, , , , , 1260Ala, Leu, His, Leu, His, Glu, Leu, Thr, Arg, His, Leu, Gly, Leu, Ser, Ala, Phe, Val, Leu, Phe, Sertccgccgccg, gcaccctcgg, caacgcgggt, cagggcaact, acgccgccgc, caacgcctac, , , , , 1320Ser, Ala, Ala, Gly, Thr, Leu, Gly, Asn, Ala, Gly, Gln, Gly, Asn, Tyr, Ala, Ala, Ala, Asn, Ala, Tyrctcgacgcgc, tggcccatcg, acgccgggcc, caggggctgc, cggcagtatc, cctcgcctgg, , , , , 1380Leu, Asp, Ala, Leu, Ala, His, Arg, Arg, Arg, Ala, Gln, Gly, Leu, Pro, Ala, Val, Ser, Leu, Ala, Trpggcatgtggc, agcaggccgc, gggaacgggg, atgaccggcc, gtctcggcga, tgccgagcag, , , , , 1440Gly, Met, Trp, Gln, Gln, Ala, Ala, Gly, Thr, Gly, Met, Thr, Gly, Arg, Leu, Gly, Asp, Ala, Glu, Glncgccggatga, cacgcggcgg, ggtggccccc, ttgtccccgg, ccgagggcat, ggagctcttc, , , , , 1500Arg, Arg, Met, Thr, Arg, Gly, Gly, Val, Ala, Pro, Leu, Ser, Pro, Ala, Glu, Gly, Met, Glu, Leu, Phegacactgcgc, tgcgtatggc, cgaacccacg, gtcctcccca, tcaaactgga, cctcggtgcg, , , , , 1560Asp, Thr, Ala, Leu, Arg, Met, Ala, Glu, Pro, Thr, Val, Leu, Pro, Ile, Lys, Leu, Asp, Leu, Gly, Alactccgcgccc, aggccgccac, cggggcggtg, cagccgttgc, tgcaccggct, ggtgccaccg, , , , , 1620Leu, Arg, Ala, Gln, Ala, Ala, Thr, Gly, Ala, Val, Gln, Pro, Leu, Leu, His, Arg, Leu, Val, Pro, Progtccgccgag, ccactcgcgc, cacggccgag, cagggcctgg, tgaccggccg, gctggcgggc, , , , , 1680Val, Arg, Arg, Ala, Thr, Arg, Ala, Thr, Ala, Glu, Gln, Gly, Leu, Val, Thr, Gly, Arg, Leu, Ala, Glygcgacccccg, aggagcggga, gcggatcctg, ctggagatgg, tccagcagga, ggccgcccgg, , , , , 1740Ala, Thr, Pro, Glu, Glu, Arg, Glu, Arg, Ile, Leu, Leu, Glu, Met, Val, Gln, Gln, Glu, Ala, Ala, Arggtcctgggac, actcggcggc, tgccacgctc, gaccccgatg, tgctgttcac, cgagatcggc, , , , , 1800Val, Leu, Gly, His, Ser, Ala, Ala, Ala, Thr, Leu, Asp, Pro, Asp, Val, Leu, Phe, Thr, Glu, Ile, Glyctggactccc, tgatggcggt, ggaactacgc, gatcgcctgg, ccaagcgcac, cgcgctgcgg, , , , , 1860Leu, Asp, Ser, Leu, Met, Ala, Val, Glu, Leu, Arg, Asp, Arg, Leu, Ala, Lys, Arg, Thr, Ala, Leu, Argttgcctccca, gctttgtctt, cgaccacccc, accctccgga, tgctggcccg, gcagctgtgg, , , , , 1920Leu, Pro, Pro, Ser, Phe, Val, Phe, Asp, His, Pro, Thr, Leu, Arg, Met, Leu, Ala, Arg, Gln, Leu, Trpgacgagctgg, agaaagccga, tacggacgct, cccgccgcat, ccgccccgac, tcccgcatcc, , , , , 1980Asp, Glu, Leu, Glu, Lys, Ala, Asp, Thr, Asp, Ala, Pro, Ala, Ala, Ser, Ala, Pro, Thr, Pro, Ala, Sergccgaaactc, ccgcatccgc, cccaaccgcc, ggagccaccc, cgtcacccgg, agccaccccg, , , , , 2040Ala, Glu, Thr, Pro, Ala, Ser, Ala, Pro, Thr, Ala, Gly, Ala, Thr, Pro, Ser, Pro, Gly, Ala, Thr, Protcacccggag, ccaccccgtc, acccggagcc, actctgccac, ccgcgcccac, cccgccttcc, , , , , 2100Ser, Pro, Gly, Ala, Thr, Pro, Ser, Pro, Gly, Ala, Thr, Leu, Pro, Pro, Ala, Pro, Thr, Pro, Pro, Sergagccgccc, aggag, 2115Gly, Ala, Ala, Gln, the Glu gene outcomes are shown and rifamycin, I types PKS, rif module 9 and 10 has higher homology, (, 40%, ),,Similitude 50%;Additionally with rifB module 4-6 homologous (39%), similitude 49%. rifPKS9-10.pro ------------------------------------------------------------ 1564gdnB-pks.pro ------------------------------------------------------------ 522rifpks4-6.pro AVRGGKFFVPRITRAEPSGAAVFRPDGTVLISGAGALGGLVARRLVERHGVRKLVLASRR 2996rifPKS9-10.pro ------------------------------------------------------------ 1564gdnB-pks.pro ------------------------------------------------------------ 522rifpks4-6.pro GRDADGVADLVADLAADVSVVACDVSDRAQVAALLDEHRPTAVVHTAGVIDAGVIETLDR 3056rifPKS9-10.pro ------------------------------------------------------------ 1564gdnB-pks.pro ------------------------------------------------------------ 522rifpks4-6.pro DRLATVFAPKVDAVRHLDELTRDRDLDAFVVYSSVSAVFMGAGSGSYAAANAFLDGLMAN 3116rifPKS9-10.pro ------------------------------------------------------------ 1564gdnB-pks.pro ------------------------------------------------------------ 522rifpks4-6.pro RRAAGLPGLSLAWGLWDQSTGMAAGTDEATRARMSRRGGLQIMTQAEGMDLFDAALSSAE 3176rifPKS9-10.pro ------------------------------------------------------------ 1564gdnB-pks.pro ------------------------------------------------------------ 522 Conserved structure domain analysis, at amino acid N end there is the attachment site (see Fig. 4) of a phosphopantetheine in 572-636 amino acids, phosphopantetheine is ACP Auxiliary bases, it carries the intermediate of aliphatic acid synthesis as a rocking arm, gone to by an enzyme on the active position of another enzyme, polyketone synthesis body.11.gdnF (Genbank indexed numbers are AF521895):Amide synthase gene, size is 1026bpDNA, and the gene initiation codon has overlapping with the termination codon of its upstream gene, is likely to be at same transcript unit.Encode the albumen being made up of 342 amino acid.Gtgggacgag, ctggagaaag, ccgatacgga, cgctcccgcc, gcatccgccc, cgactcccgc, , , , , 60Val, Gly, Arg, Ala, Gly, Glu, Ser, Arg, Tyr, Gly, Arg, Ser, Arg, Arg, Ile, Arg, Pro, Asp, Ser, Argatccgccgaa, actcccgcat, ccgccccaac, cgccggagcc, accccgtcac, ccggagccac, , , , , 120Ile, Arg, Arg, Asn, Ser, Arg, Ile, Arg, Pro, Asn, Arg, Arg, Ser, His, Pro, Val, Thr, Arg, Ser, Hiscccgtcaccc, ggagccaccc, cgtcacccgg, agccactctg, ccacccgcgc, ccaccccgcc, , , , , 180Pro, Val, Thr, Arg, Ser, His, Pro, Val, Thr, Arg, Ser, His, Ser, Ala, Thr, Arg, Ala, His, Pro, Alattccggagcc, gcccaggagt, gagccctgcc, cagcccagca, caactccact, cggcagaccg, , , , , 240Phe, Arg, Ser, Arg, Pro, Gly, Val, Ser, Pro, Ala, Gln, Pro, Ser, Thr, Thr, Pro, Leu, Gly, Arg, Progcaccgaccg, aagcggcaa, gaaagggatc, gcacatgttc, agcacggacac, gtacctggcg, , , , , 300Ala, Pro, Thr, Glu, Ala, Ala, Arg, Lys, Gly, Ser, His, Met, Phe, Ser, Thr, Asp, Thr, Tyr, Leu, Alacatctggggt, ttccccagc, cgcccgcccc, caccctgccg, aacctccggca, gttgcaccgc, , , , , 360His, Leu, Gly, Phe, Pro, Gln, Pro, Pro, Ala, Pro, Thr, Leu, Pro, Asn, Leu, Arg, Gln, Leu, His, Argggccatctga, tggcggtcc, cttacgacac, caaccacacc, caccgcctcag, cgcggagaac, , , , , 420Gly, His, Leu, Met, Ala, Val, Pro, Tyr, Asp, Thr, Asn, His, Thr, His, Arg, Leu, Ser, Ala, Glu, Asnatggccgaca, tcgatatcg, acaaggcatt, cgaggccatc, gtgccgaccgg, cgccggtggc, , , , , 480Met, Ala, Asp, Ile, Asp, Ile, Asp, Lys, Ala, Phe, Glu, Ala, Ile, Val, Pro, Thr, Gly, Ala, Gly, Glyatgtgcctgg, agctgaaca, ccctgttcgc, ccagttgctc, cgcgagctggg, ctatgacctg, , , , , 540Met, Cys, Leu, Glu, Leu, Asn, Thr, Leu, Phe, Ala, Gln, Leu, Leu, Arg, Glu, Leu, Gly, Tyr, Asp, Leugacgtcatca, gcggaggca, cgtatctgcc, cggtgacatc, ttcgcccccga, ccccgagcac, , , , , 600Asp, Val, Ile, Ser, Gly, Gly, Thr, Tyr, Leu, Pro, Gly, Asp, Ile, Phe, Ala, Pro, Asp, Pro, Glu, Hisatgctgatgc, tcgtccgtat, cgacgggcag, gagtggctgg, ccgatgtggg, gcacgccggt, , , , , 660Met, Leu, Met, Leu, Val, Arg, Ile, Asp, Gly, Gln, Glu, Trp, Leu, Ala, Asp, Val, Gly, His, Ala, Glyctctgtttca, ccgagccgct, gcgcctgtcc, gaggaggtgc, agtggcagta, cggctgcgct, , , , , 720Leu, Cys, Phe, Thr, Glu, Pro, Leu, Arg, Leu, Ser, Glu, Glu, Val, Gln, Trp, Gln, Tyr, Gly, Cys, Alattccggctga, tccggcggga, tggctatctc, gtgctccagg, ccaagaccct, ggaccacgac, , , , , 780Phe, Arg, Leu, Ile, Arg, Arg, Asp, Gly, Tyr, Leu, Val, Leu, Gln, Ala, Lys, Thr, Leu, Asp, His, Asptggcgcacca, cctaccgctt, caccaccgag, cccaggacct, atgacgcctg, ggccggggtc, , , , , 840Trp, Arg, Thr, Thr, Tyr, Arg, Phe, Thr, Thr, Glu, Pro, Arg, Thr, Tyr, Asp, Ala, Trp, Ala, Gly, Valggtgagggca, atggcccggc, catcctggcg, gcgatgcgcc, gacgcaggcg, cgccatcgac, , , , , 900Gly, Glu, Gly, Asn, Gly, Pro, Ala, Ile, Leu, Ala, Ala, Met, Arg, Arg, Arg, Arg, Arg, Ala, Ile, Aspaaggggcagg, tcttcctcac, caacaacatg, ttcacgatcg, tggagaacgg, ccatgagaag, , , , , 960Lys, Gly, Gln, Val, Phe, Leu, Thr, Asn, Asn, Met, Phe, Thr, Ile, Val, Glu, Asn, Gly, His, Glu, Lysgtcaccctcc, tcgtcgatcc, ggaacggcgc, gcccaggtgc, tcgacacgta, ctgggacggt, , , , , 1020Val, Thr, Leu, Leu, Val, Asp, Pro, Glu, Arg, Arg, Ala, Gln, Val, Leu, Asp, Thr, Tyr, Trp, Asp, Glycgcgac, , , , , , , , , , , , , , , , , , , , 1026Arg, the Asp gene outcomes, which are shown with rifamycin amide synthases, to be had compared with high homology, (, 37%, ), similitude 50%;gdnF.pro VGRAGESRYGRSRRIRPDSRIRRNSRIRPNRRSHPVTRSHPVTRSHPVTRSHSATRAHPA 60RIFF.pro ------------------------------------------------------------ 1 Its aromatic amine acyltransferase also with a variety of sources is homologous in addition;Amino acid sequence analysis is shown; in the 111-275 amino acid position domain of acyltransferase containing N- (see Fig. 4); this domain is probably assembling structure domain of the Ansamycin specifically for fragrant starter unit, and the function of the gene outcome is the lactam ring structure for forming uniqueness on AHBA amino with this by the way that PKS acyl group is transferred to.12.gdnG (Genbank indexed numbers are AF521895):Monooxygenase gene, size is 1224bp DNA, encodes the albumen being made up of 408 amino acid.Atgaaattcg, gtttgctgta, cggggcgcag, ctgccccgac, cctggaccca, ggactccgaa, , , , , 60Met, Lys, Phe, Gly, Leu, Leu, Tyr, Gly, Ala, Gln, Leu, Pro, Arg, Pro, Trp, Thr, Gln, Asp, Ser, Glucaccgcctgt, tcaacgagat, gttggacgag, atcgagctgg, ccgaccggct, gggcttcgac, , , , , 120His, Arg, Leu, Phe, Asn, Glu, Met, Leu, Asp, Glu, Ile, Glu, Leu, Ala, Asp, Arg, Leu, Gly, Phe, Aspcatgtgtggt, gtcctgagca, ccacttcctg, gaggagtact, cgcacatgtc, cgcgccggag, , , , , 180His, Val, Trp, Cys, Pro, Glu, His, His, Phe, Leu, Glu, Glu, Tyr, Ser, His, Met, Ser, Ala, Pro, Glugcgttcctcg, gcgcggtcag, ccagcgcacc, agccgcatcc, gcatcggcca, cgcggtggcc, , , , , 240Ala, Phe, Leu, Gly, Ala, Val, Ser, Gln, Arg, Thr, Ser, Arg, Ile, Arg, Ile, Gly, His, Ala, Val, Alactgatgcctc, cggcgttcaa, tccgacggca, cgggtcgccg, agcggatcgc, cacgctggac, , , , , 300Leu, Met, Pro, Pro, Ala, Phe, Asn, Pro, Thr, Ala, Arg, Val, Ala, Glu, Arg, Ile, Ala, Thr, Leu, Aspctgctctccg, acggccgggt, ggacttcggc, acgggcgagt, ccaccacccc, caccgagctg, , , , , 360Leu, Leu, Ser, Asp, Gly, Arg, Val, Asp, Phe, Gly, Thr, Gly, Glu, Ser, Thr, Thr, Pro, Thr, Glu, Leuggcggattcg, gcgtggagcg, ctccgtgaaa, cgggaccagt, gggcggaggc, ggtggacgcc, , , , , 420Gly, Gly, Phe, Gly, Val, Glu, Arg, Ser, Val, Lys, Arg, Asp, Gln, Trp, Ala, Glu, Ala, Val, Asp, Alagtcgcccgga, tgttcgtcga, ggagcccttc, gccggatacg, agggcaagta, cgtgtccgcc, , , , , 480Val, Ala, Arg, Met, Phe, Val, Glu, Glu, Pro, Phe, Ala, Gly, Tyr, Glu, Gly, Lys, Tyr, Val, Ser, Alaccgatccgca, atgtgctgcc, caagacccgg, cagaagccac, atccgccgat, gtggatggcc, , , , , 540Pro, Ile, Arg, Asn, Val, Leu, Pro, Lys, Thr, Arg, Gln, Lys, Pro, His, Pro, Pro, Met, Trp, Met, Alatgcgggaacc, gggacgcgat, ccgcaccgcg, gccgccaagg, ggctcggcgc, gctgaacttc, , , , , 600cys, Gly, Asn, Arg, Asp, Ala, Ile, Arg, Thr, Ala, Ala, Ala, Lys, Gly, Leu, Gly, Ala, Leu, Asn, Phetccttcttcg, ggccggcgga, gaccaagaag, tgggtcgacg, cctactactc, gggcatcgaa, , , , , 660Ser, Phe, Phe, Gly, Pro, Ala, Glu, Thr, Lys, Lys, Trp, Val, Asp, Ala, Tyr, Tyr, Ser, Gly, Ile, Glutcggcggact, gtgtgcccgc, cgcgttcgcc, gtcaacgcac, agatcgccgc, gaccatcccg, , , , , 720ser, Ala, Asp, Cys, Val, Pro, Ala, Ala, Phe, Ala, Val, Asn, Ala, Gln, Ile, Ala, Ala, Thr, Ile, Proatgttctgcc, accgggacga, gaccacggcc, gtggaacgcg, ccgtcgacgg, cgtccagttc, , , , , 780Met, Phe, Cys, His, Arg, Asp, Glu, Thr, Thr, Ala, Val, Glu, Arg, Ala, Val, Asp, Gly, Val, Gln, Phettcaacttcg, gcctcggctt, ctacgcgggc, ttcggcaccg, ccgcaccggc, ccgcacccgg, , , , , 840Phe, Asn, Phe, Gly, Leu, Gly, Phe, Tyr, Ala, Gly, Phe, Gly, Thr, Ala, Ala, Pro, Ala, Arg, Thr, Argctgtgggagg, agttccagcg, cgaccgcgac, aagcagggca, tgggccgctc, ctccttcggc, , , , , 900Leu, Trp, Glu, Glu, Phe, Gln, Arg, Asp, Arg, Asp, Lys, Gln, Gly, Met, Gly, Arg, Ser, Ser, Phe, Glyaagcccggaa, tgccgctggg, caatccggcc, cggggcgcgg, tgggcactcc, gcaccagata, , , , , 960Lys, Pro, Gly, Met, Pro, Leu, Gly, Asn, Pro, Ala, Arg, Gly, Ala, Val, Gly, Thr, Pro, His, Gln, Ilecgcgacttcc, tgcggctgca, cgaggaggcc, ggactggacc, aggcgatctt, cctcgtccag, , , , , 1020Arg, Asp, Phe, Leu, Arg, Leu, His, Glu, Glu, Ala, Gly, Leu, Asp, Gln, Ala, Ile, Phe, Leu, Val, Glnggcggtggca, cccggcacga, gcacatccgc, gaatcgctgg, agctcttcgc, caatgaggtg, , , , , 1080Gly, Gly, Gly, Thr, Arg, His, Glu, His, Ile, Arg, Glu, Ser, Leu, Glu, Leu, Phe, Ala, Asn, Glu, Valatgcccgagt, tcaaggagcg, cgatgaggcc, gccgtacggc, tgaagacggc, ccggctccag, , , , , 1140Met, Pro, Glu, Phe, Lys, Glu, Arg, Asp, Glu, Ala, Ala, Val, Arg, Leu, Lys, Thr, Ala, Arg, Leu, Glncccgccatcg, acgccgccat, ggcccggcgc, gagccgccac, ggacggccga, ccccgactac, , , , , 1200Pro, Ala, Ile, Asp, Ala, Ala, Met, Ala, Arg, Arg, Glu, Pro, Pro, Arg, Thr, Ala, Asp, Pro, Asp, Tyrtcatccccgc, ccggagccag, agc, , , , , , , , , , , , , , , 1224Ile, Ile, Pro, Ala, Arg, Ser, Gln, the Ser gene outcomes show homologous with rifamycin monooxygenase Rif17, (, 26%, ),,Similitude (43%),.This gene outcome may relate to the amide structure formation (Silakowski, B.et al.J.Biol.Chem.1999.274 (52), 37391-37399) of end.13.gdnH (Genbank indexed numbers are AF521895):Cytochrome P450, hydroxylation enzyme gene, size are 1239bp, encode the albumen being made up of 413 amino acid.Gtgtccgggc, gccacttcga, acaaggagaa, cgtggtaccg, ccatggctga, cacccccgaa, , , , , 60Val, Ser, Gly, Arg, His, Phe, Glu, Gln, Gly, Glu, Arg, Gly, Thr, Ala, Met, Ala, Asp, Thr, Pro, Glugaagaactcc, gcatcctcga, cccgcagtcc, gtcgcgcagg, agctgcgcaa, gcacggcccg, , , , , 120Glu, Glu, Leu, Arg, Ile, Leu, Asp, Pro, Gln, Ser, Val, Ala, Gln, Glu, Leu, Arg, Lys, His, Gly, Procctcggcaga, tcacgatgca, cggcaccacg, gcgtggctcg, tctcccggta, cgaggaggtc, , , , , 180Pro, Arg, Gln, Ile, Thr, Met, His, Gly, Thr, Thr, Ala, Trp, Leu, Val, Ser, Arg, Tyr, Glu, Glu, Valcgggactgtc, tcggacaccc, cggaatgagc, ccggccgccg, cctacgccgc, ctcccagggc, , , , , 240Arg, Asp, Cys, Leu, Gly, His, Pro, Gly, Met, Ser, Pro, Ala, Ala, Ala, Tyr, Ala, Ala, Ser, Gln, Glycagaccaatc, cggtgagcg, ggttgttcgag, gacacggtgg, ccggtaccaa, tccgccccag, , , , , 300Gln, Thr, Asn, Pro, Val, Ser, Gly, Leu, Phe, Glu, Asp, Thr, Val, Ala, Gly, Thr, Asn, Pro, Pro, Glncacacccggc, tgcgcaggc, tgctggccaa, ggcgttcacgg, tacgcagagt, ggagagtctg, , , , , 360His, Thr, Arg, Leu, Arg, Arg, Leu, Leu, Ala, Lys, Ala, Phe, Thr, Val, Arg, Arg, Val, Glu, Ser, Leucggccacggg, tgcaggaga, tcaccgacac, actgctggacc, ggatcgccgt, cgacggccgg, , , , , 420Arg, Pro, Arg, Val, Gln, Glu, Ile, Thr, Asp, Thr, Leu, Leu, Asp, Arg, Ile, Ala, Val, Asp, Gly, Arggccgacctcg, tcagcgcgc, tggccattcc, gctgcccatgc, aggtgatctg, cgaactcctc, , , , , 480Ala, Asp, Leu, Val, Ser, Ala, Leu, Ala, Ile, Pro, Leu, Pro, Met, Gln, Val, Ile, Cys, Glu, Leu, Leuggtgtgccca, tcgccgacc, gcaccgaatt, ccaccagtgg, gccgatctgat, gctcacgccc, , , , , 540Gly, Val, Pro, Ile, Ala, Asp, Arg, Thr, Glu, Phe, His, Gln, Trp, Ala, Asp, Leu, Met, Leu, Thr, Proccgctggacc, cggacaccg, ccgcgcgttc, ccaggacgcc, tccgccaagct, gtggacgtat, , , , , 600Pro, Leu, Asp, Pro, Asp, Thr, Ala, Ala, Arg, Ser, Gln, Asp, Ala, Ser, Ala, Lys, Leu, Trp, Thr, Tyratggaggacc, tcgccgagg, ccaggcggaa, ggccccggag, gacgacctgat, cagcgatctg, , , , , 660Met, Glu, Asp, Leu, Ala, Glu, Ala, Arg, Arg, Lys, Ala, Pro, Glu, Asp, Asp, Leu, Ile, Ser, Asp, Leuatgtccgcac, acgaggacgac, cggctcagcc, accgcgaggt, ggtcgccacc, gcccggatg, , , , , 720Met, Ser, Ala, His, Glu, Asp, Asp, Arg, Leu, Ser, His, Arg, Glu, Val, Val, Ala, Thr, Ala, Arg, Metatgctgatcg, cggggtacga, gctgaccggc, agcttcatca, gcaacgcggt, tttctcgctg, , , , , 780Met, Leu, Ile, Ala, Gly, Tyr, Glu, Leu, Thr, Gly, Ser, Phe, Ile, Ser, Asn, Ala, Val, Phe, Ser, Leuctgtcccagc, ccgaccagat, ggaactgctg, cgcaaggacc, ccgagctggc, cgggcgcggt, , , , , 840Leu, Ser, Gln, Pro, Asp, Gln, Met, Glu, Leu, Leu, Arg, Lys, Asp, Pro, Glu, Leu, Ala, Gly, Arg, Glyctggaggagc, tgctccggca, cgccgggccg, ggcattctca, tcgtgcgttt, cgccaacgag, , , , , 900Leu, Glu, Glu, Leu, Leu, Arg, His, Ala, Gly, Pro, Gly, Ile, Leu, Ile, Val, Arg, Phe, Ala, Asn, Glugacgtggaga, tcggctccgt, atccatccgc, gccggcgacc, aggtgctcct, ggacatggac, , , , , 960Asp, Val, Glu, Ile, Gly, Ser, Val, Ser, Ile, Arg, Ala, Gly, Asp, Gln, Val, Leu, Leu, Asp, Met, Aspgccgcacact, ccgacccggc, gcacttcacc, gacggcgagc, ggctggacct, cacgagggac, , , , , 1020Ala, Ala, His, Ser, Asp, Pro, Ala, His, Phe, Thr, Asp, Gly, Glu, Arg, Leu, Asp, Leu, Thr, Arg, Asptcggccgtac, acctccagtt, cggccatggc, atccactact, gcatcggcgc, gccgctggcc, , , , , 1080Ser, Ala, Val, His, Leu, Gln, Phe, Gly, His, Gly, Ile, His, Tyr, Cys, Ile, Gly, Ala, Pro, Leu, Alaagggtggagg, ggcagatcgc, cctggagagc, ctggtgcggc, ggttccccgg, gcttcggctg, , , , , 1140Arg, Val, Glu, Gly, Gln, Ile, Ala, Leu, Glu, Ser, Leu, Val, Arg, Arg, Phe, Pro, Gly, Leu, Arg, Leuagcgttcccg, ccgccgagat, cagccatagc, aagaacccgt, tcatccgctc, gctgaccgcg, , , , , 1200Ser, Val, Pro, Ala, Ala, Glu, Ile, Ser, His, Ser, Lys, Asn, Pro, Phe, Ile, Arg, Ser, Leu, Thr, Alactgcccgtcg, agttcgaggc, tcagcagcc, cgtagcgggg, , , , , , , , , , , 1239Leu, Pro, Val, Glu, Phe, Glu, Ala, Gln, Gln, Pro, Val, Ala, the Gly gene outcomes are shown and Cytochrome P450,6- deoxyerythronolides B (6-DEB) hydroxylases EryF has higher homology (38%),Similitude 56%.ERYF.pro --- the amino acid sequence analysis of -404gdnH.pro PVAG 413 is shown, is contained Cytochrome P450 domain (see Fig. 5).GdnH and gdnG is structural modification gene, may participate in oxidative modification step during the formation of geldanamycin benzoquinones.(14.orf1 Genbank indexed number AF521895):DNA repair protein, size is 678bp, encode the albumen being made up of 226 amino acid.Atgaacgccc, tgatcccccg, tccacgcctc, gaggtggccc, ccggcgccgt, ccatgtgccg, , , , , 60Met, Asn, Ala, Leu, Ile, Pro, Arg, Pro, Arg, Leu, Glu, Val, Ala, Pro, Gly, Ala, Val, His, Val, Proagctggctca, ccctcgaaca, gcagcgggag, ctggtcctcg, cctgccgggg, ctgggccacc, , , , , 120Ser, Trp, Leu, Thr, Leu, Glu, Gln, Gln, Arg, Glu, Leu, Val, Leu, Ala, Cys, Arg, Gly, Trp, Ala, Thrggcccggtcc, cgatccggca, caccaagctg, ccgcgcgggg, gcgtcatgtc, ggtgcgcacg, , , , , 180Gly, Pro, Val, Pro, Ile, Arg, His, Thr, Lys, Leu, Pro, Arg, Gly, Gly, Val, Met, Ser, Val, Arg, Thrgtgtgcatcg, gctggcactg, gcagccctac, gcctacaccc, gcaccgccga, cgatgtgaac, , , , , 240Val, cys, Ile, Gly, Trp, His, Trp, Gln, Pro, Tyr, Ala, Tyr, Thr, Arg, Thr, Ala, Asp, Asp, Val, Asnggcgcccggg, tcgccgaatt, ccccgactgg, atggtcgagt, tgggccgtcg, cgccctggtc, , , , , 300Gly, Ala, Arg, Val, Ala, Glu, Phe, Pro, Asp, Trp, Met, Val, Glu, Leu, Gly, Arg, Arg, Ala, Leu, Valgacgcgtacg, acgacgagac, ggccggtgag, gggtacaccc, ccgacaccgc, gctcatcaac, , , , , 360Asp, Ala, Tyr, Asp, Asp, Glu, Thr, Ala, Gly, Glu, Gly, Tyr, Thr, Pro, Asp, Thr, Ala, Leu, Ile, Asnttctacgacg, cccaggcgaa, gctgggcatg, caccaggaca, aggacgagag, gtcatccgcc, , , , , 420Phe, Tyr, Asp, Ala, Gln, Ala, Lys, Leu, Gly, Met, His, Gln, Asp, Lys, Asp, Glu, Arg, Ser, Ser, Alaccggtggtct, cgctcaccat, cggcgacagc, tgtgtcttcc, gcttcggcaa, caccgagacc, , , , , 480Pro, Val, Val, Ser, Leu, Thr, Ile, Gly, Asp, Ser, Cys, Val, Phe, Arg, Phe, Gly, Asn, Thr, Glu, Thrcgtaccaagc, cgtacaccga, cctcgaactc, gcttccgggga, tctgttcgt, cttcggaggc, , , , , 540Arg, Thr, Lys, Pro, Tyr, Thr, Asp, Leu, Glu, Leu, Ala, Ser, Gly, Asp, Leu, Phe, Val, Phe, Gly, Glyccctcccgct, acgcctatca, cgccgtcccc, aggatcctgc, ccggaaccgg, tgacccggcc, , , , , 600Pro, Ser, Arg, Tyr, Ala, Tyr, His, Ala, Val, Pro, Arg, Ile, Leu, Pro, Gly, Thr, Gly, Asp, Pro, Alaaccggactga, agtccgggcg, gctgaacatc, accatgcggg, tcaccggtct, ggccgatccc, , , , , 660Thr, Gly, Leu, Lys, Ser, Gly, Arg, Leu, Asn, Ile, Thr, Met, Arg, Val, Thr, Gly, Leu, Ala, Asp, Procagtcgtcag, tcgtcccg, 678Gln, Ser, Ser, Val, Val, the Pro gene outcomes are shown and Streptomyces, coelicolor, A3, (, 2, ), the DNA repair protein of middle supposition, (, Drep, ), there is high homology, (, 60%, ),,Similitude 70%,It is also homologous (42%) with the DNA repair protein (Cg10) that is alkylated in Corynebacterium glutamicum,Similitude 56%. (15.orf2 Genbank indexed number AF521895):Unknown Function albumen, size is 726bp DNA, encodes the albumen being made up of 242 amino acid.Atgagcacca, cgaacgacac, cgcacgcatc, catcagcgcg, tggccgcggc, cgactggcca, , , , , 60Met, Ser, Thr, Thr, Asn, Asp, Thr, Ala, Arg, Ile, His, Gln, Arg, Val, Ala, Ala, Ala, Asp, Trp, Procagctggccg, aggagctgga, cacctacggg, tgcgcgctca, ctccacggct, gctgaccccc, , , , , 120Gln, Leu, Ala, Glu, Glu, Leu, Asp, Thr, Tyr, Gly, Cys, Ala, Leu, Thr, Pro, Arg, Leu, Leu, Thr, Progcccagtgcg, cccgcatcgc, cgggctgtac, gggcaggacg, agcagttcag, gaacacgatc, , , , , 180Ala, Gln, Cys, Ala, Arg, Ile, Ala, Gly, Leu, Tyr, Gly, Gln, Asp, Glu, Gln, Phe, Arg, Asn, Thr, Ilegacatggccc, gccaccgctt, cggctccgga, cagtaccgct, acttcaccca, tgacctgccc, , , , , 240Asp, Met, Ala, Arg, His, Arg, Phe, Gly, Ser, Gly, Gln, Tyr, Arg, Tyr, Phe, Thr, His, Asp, Leu, Progaaccggtgg, ccgagctgcg, cgccgcgctc, tatccgcggc, tgctgaccat, cgcgcgtgac, , , , , 300Glu, Pro, Val, Ala, Glu, Leu, Arg, Ala, Ala, Leu, Tyr, Pro, Arg, Leu, Leu, Thr, Ile, Ala, Arg, Asptgggcggagc, ggctcggccg, cccggcgccc, tggccggaca, gcctcgagaa, gtggctggcc, , , , , 360Trp, Ala, Glu, Arg, Leu, Gly, Arg, Pro, Ala, Pro, Trp, Pro, Asp, Ser, Leu, Glu, Lys, Trp, Leu, Alaatgtgtcatg, aggccggaca, ggaccgctcc, gcgcagatcc, tgctgcgcta, cggccccggc, , , , , 420Met, Cys, His, Glu, Ala, Gly, Gln, Asp, Arg, Ser, Ala, Gln, Ile, Leu, Leu, Arg, Tyr, Gly, Pro, Glygactggaacg, ccctgcaccg, ggacgtattc, ggcgacatgc, tcttcccgct, ccaggtggtg, , , , , 480Asp, Trp, Asn, Ala, Leu, His, Arg, Asp, Val, Phe, Gly, Asp, Met, Leu, Phe, Pro, Leu, Gln, Val, Valatcgggctcg, acgcgtacggc, acggactac, acgggcgggg, agttcctgct, ggtcgagcag, , , , , 540Ile, Gly, Leu, Asp, Ala, Tyr, Gly, Thr, Asp, Tyr, Thr, Gly, Gly, Glu, Phe, Leu, Leu, Val, Glu, Glncggccccgcg, cccagtcccgg, ggcaccacg, accgtcctcc, agcagggcca, cggcctgatc, , , , , 600Arg, Pro, Arg, Ala, Gln, Ser, Arg, Gly, Thr, Thr, Thr, Val, Leu, Gln, Gln, Gly, His, Gly, Leu, Ilettcaccaccc, gtgaccgtccc, gtggccacc, aagcgcggct, ggtcggccgg, tgtgatgcgg, , , , , 660Phe, Thr, Thr, Arg, Asp, Arg, Pro, Val, Ala, Thr, Lys, Arg, Gly, Trp, Ser, Ala, Gly, Val, Met, Argcacggggtca, gcacggtgcgt, tccgggcgc, cgccacgcat, tggggctggt, cttccacgac, , , , , 720His, Gly, Val, Ser, Thr, Val, Arg, Ser, Gly, Arg, Arg, His, Ala, Leu, Gly, Leu, Val, Phe, His, Aspgccgcc, 726Ala, Ala16.orf3, (, Genbank indexed numbers AF521895, ),:The transmethylase of anti-DNA damnification, size is 504bpDNA, encodes the albumen being made up of 168 amino acid.Tgacggtcc, acacgacgat, cgacagcccg, ctcggcgagc, tgctgctggt, gggcgaggag, , , , , 60Met, Thr, Val, His, Thr, Thr, Ile, Asp, Ser, Pro, Leu, Gly, Glu, Leu, Leu, Leu, Val, Gly, Glu, Glutccgccaccg, cgccgggggg, caccgcactc, atctccctgt, ccgtgcccgg, ccagaagggc, , , , , 120Ser, Ala, Thr, Ala, Pro, Gly, Gly, Thr, Ala, Leu, Ile, Ser, Leu, Ser, Val, Pro, Gly, Gln, Lys, Glyggggccgtcg, tccaggacgg, ttggagcgag, gatgccgagg, cgttcaccga, gatcgtctcc, , , , , 180Gly, Ala, Val, Val, Gln, Asp, Gly, Trp, Ser, Glu, Asp, Ala, Glu, Ala, Phe, Thr, Glu, Ile, Val, Sercagttgcgct, cctacttcga, cggcgagcgc, acccgcttcg, acatcgagtg, cgtcgagggc, , , , , 240Gln, Leu, Arg, Ser, Tyr, Phe, Asp, Gly, Glu, Arg, Thr, Arg, Phe, Asp, Ile, Glu, Cys, Val, Glu, Glyggtacggact, tccagcgcag, ggtctggcag, gcgctggagg, ccattccgta, cggcaccact, , , , , 300Gly, Thr, Asp, Phe, Gln, Arg, Arg, Val, Trp, Gln, Ala, Leu, Glu, Ala, Ile, Pro, Tyr, Gly, Thr, Thrgtcagctacg, gcgacatcgc, ccggcagatc, ggcgccccgc, gcacggccgt, ccgctccgtc, , , , , 360Val, Ser, Tyr, Gly, Asp, Ile, Ala, Arg, Gln, Ile, Gly, Ala, Pro, Arg, Thr, Ala, Val, Arg, Ser, Valggcaccgcga, tcggccgcaa, tccactgctg, gtcgtgcggc, cctgccaccg, ggtcatcggc, , , , , 420Gly, Thr, Ala, Ile, Gly, Arg, Asn, Pro, Leu, Leu, Val, Val, Arg, Pro, Cys, His, Arg, Val, Ile, Glygccaccggcg, cactgaccgg, ctatgcgggc, ggactggagc, gcaagcagcg, actcctcgtt, , , , , 480Ala, Thr, Gly, Ala, Leu, Thr, Gly, Tyr, Ala, Gly, Gly, Leu, Glu, Arg, Lys, Gln, Arg, Leu, Leu, ValCacgagggcg, ccctccagac, cgcc, 504His, Glu, Gly, Ala, Leu, Gln, Thr, the Ala gene outcomes are shown and Streptomyces, coelicolor, A3, (, 2, ), methylate DNA-, [, albumen, ],-cysteine-sulphur-transmethylase, (, S_MDP, ), there is high homology, (, 53%, ),,Similitude 61%;It also shows homologous (45%) with 6- oxygen-methyl guanine-transmethylase (M_OGT) of mycobacteria, similitude 56%.S_MDP.pro LPATPR 186M_OGT.pro LFD--- 165orf3-.pro --- --- 168 enzymes are by by the transalkylation of oxygen -6 to the cysteine residues of the enzyme, so that the guanine being alkylated on DNA plerosis.Geldanamycin structural analysis shows, it contains alkyl and alkyl on benzoquinones is more active, it is possible to DNA is alkylated to the damage for causing DNA, the possible gene is acted on the orf1 self-protections for participating in body antibody Monoclonal jointly.(17.orf4 Genbank indexed number AF521895):For a new gene, size is 618bpDNA, encodes the albumen being made up of 206 amino acid.Atgcacgagg, gacacggcca, ccaggagtac, atcgtcacgt, ccgaccccga, ggcggtggcc, , , , , 60Met, His, Glu, Gly, His, Gly, His, Gln, Glu, Tyr, Ile, Val, Thr, Ser, Asp, Pro, Glu, Ala, Val, Alacgtgtccggg, cctccttgct, gcgcaccctg, ccagtggccg, catgggccgg, ggtggccagt, , , , , 120Arg, Val, Arg, Ala, Ser, Leu, Leu, Arg, Thr, Leu, Pro, Val, Ala, Ala, Trp, Ala, Gly, Val, Ala, Sergccgtggtca, tcgtctcggc, cgtggccctc, gtcctcttcg, ccctcggcca, cagcgcctcc, , , , , 180Ala, Val, Val, Ile, Val, Ser, Ala, Val, Ala, Leu, Val, Leu, Phe, Ala, Leu, Gly, His, Ser, Ala, Sercggctgtggg, tcctggtgtt, cgcctggccc, gcggcgttcc, tcggctatga, cgccaggcgc, , , , , 240Arg, Leu, Trp, Val, Leu, Val, Phe, Ala, Trp, Pro, Ala, Ala, Phe, Leu, Gly, Tyr, Asp, Ala, Arg, Argcgattcgccg, atatacggcg, gctgaagcgg, acctgggcgg, cgaaagaggt, gtccccggtg, , , , , 300Arg, Phe, Ala, Asp, Ile, Arg, Arg, Leu, Lys, Arg, Thr, Trp, Ala, Ala, Lys, Glu, Val, Ser, Pro, Valgcgatgcgcc, tctccgccga, gggcctgcgc, tgcgccatcg, actccgcccc, ggagcccgtt, , , , , 360Ala, Met, Arg, Leu, Ser, Ala, Glu, Gly, Leu, Arg, Cys, Ala, Ile, Asp, Ser, Ala, Pro, Glu, Pro, Valttcctcccct, ggtccgcgat, cgcccaggtg, cgggtgacgg, gccagggcct, cagcacggtg, , , , , 420Phe, Leu, Pro, Trp, Ser, Ala, Ile, Ala, Gln, Val, Arg, Val, Thr, Gly, Gln, Gly, Leu, Ser, Thr, Valcgggtcgatc, tcgcccccgg, cgtgtccgcc, accacccccg, gggtcagcgg, gctggagcag, , , , , 480Arg, Val, Asp, Leu, Ala, Pro, Gly, Val, Ser, Ala, Thr, Thr, Pro, Gly, Val, Ser, Gly, Leu, Glu, Glncccgaggccc, ggatgcgcat, gcggcgcgcc, tggaacggcg, ggatgcggct, gcgcttcacc, , , , , 540Pro, Glu, Ala, Arg, Met, Arg, Met, Arg, Arg, Ala, Trp, Asn, Gly, Gly, Met, Arg, Leu, Arg, Phe, Thrgtctacgccc, tccgccagcc, gatcagcgag, atcgaccagg, ctctcggcca, cttctcgaac, , , , , 600Val, Tyr, Ala, Leu, Arg, Gln, Pro, Ile, Ser, Glu, Ile, Asp, Gin, Ala, Leu, Gly, His, Phe, Ser, Asngggcggatcg, gtatccgc, , , , , , , , , , , , , , , , , 618Gly, Arg, Ile, Gly, Ile, Arg18.orf5, (, Genbank indexed numbers AF521895, ),:There is correlation gene with transcriptional regulatory, size is 2721bpDNA, encode the albumen being made up of 907 amino acid.Atgggaggac, gtgctcgtcc, ggctcggcga, cggctggggc, ccctgtcgta, tacgcgaagc, , , , , 60Met, Gly, Gly, Arg, Ala, Arg, Pro, Ala, Arg, Arg, Arg, Leu, Gly, Pro, Leu, Ser, Tyr, Thr, Arg, Sergtcgccggg, caggcgttca, tcttgcagct, tctgctgatc, ctggttctgg, tggccgcggcg, , , , , 120Val, Ala, Gly, Gln, Ala, Phe, Ile, Leu, Gln, Leu, Leu, Leu, Ile, Leu, Val, Leu, Val, Ala, Ala, Alagtggtggcc, gtcgcagcgg, atgcccggag, ccacagcacg, accgacgctc, gccggcgatcc, , , , , 180Val, Val, Ala, Val, Ala, Ala, Asp, Ala, Arg, Ser, His, Ser, Thr, Thr, Asp, Ala, Arg, Arg, Arg, Serctcgcggtc, gccgagacct, tggcacactc, ccccggaatg, gcccgggccc, tgaccagcgac, , , , , 240Leu, Ala, Val, Ala, Glu, Thr, Leu, Ala, His, Ser, Pro, Gly, Met, Ala, Arg, Ala, Leu, Thr, Ser, Aspcggccgacg, tcgctgctgg, agtcgcatgc, ggaggcggcg, cggaagagatc, aggcgtcgac, , , , , 300Arg, Pro, Thr, Ser, Leu, Leu, Glu, Ser, His, Ala, Glu, Ala, Ala, Arg, Lys, Arg, Ser, Gly, Val, Aspagcgtcgtgg, tgttcaaca, ctcatggcat, ccgcctcacc, caccccgagaa, ggcattgatc, , , , , 360Ser, Val, Val, Val, Phe, Asn, Thr, His, Gly, Ile, Arg, Leu, Thr, His, Pro, Glu, Lys, Ala, Leu, Ileggcaagcgga, tcgtcggac, cggccgggct, ggtgcgggac, gagctgaaagg, caagacgatc, , , , , 420Gly, Lys, Arg, Ile, Val, Gly, Pro, Ala, Gly, Leu, Val, Arg, Asp, Glu, Leu, Lys, Gly, Lys, Thr, Ileacggagtcct, tccaggcca, gccagggccc, gtccgtggtc, tcggcggtccc, cgtcaccagg, , , , , 480Thr, Glu, Ser, Phe, Gln, Ala, Ser, Gln, Gly, Pro, Ser, Val, Val, Ser, Ala, Val, Pro, Val, Thr, Arggccgacggca, ccttcctcg, gcggtgtgtc, cgtcggggtc, aagatcgcgag, cgtgaacagc, , , , , 540Ala, Asp, Gly, Thr, Phe, Leu, Gly, Gly, Val, Ser, Val, Gly, Val, Lys, Ile, Ala, Ser, Val, Asn, Sergaggtggacc, gtcggctac, cgctgctgct, cggcagtggc, accggggcact, ggccctggcc, , , , , 600Glu, Val, Asp, Arg, Arg, Leu, Pro, Leu, Leu, Leu, Gly, Ser, Gly, Thr, Gly, Ala, Leu, Ala, Leu, Alatcgggcgggg, cggcgctga, tgagcaggcg, ggtgcggcgg, cagacccacgg, cctgggcgcc, , , , , 660Ser, Gly, Gly, Ala, Ala, Leu, Met, Ser, Arg, Arg, Val, Arg, Arg, Gln, Thr, His, Gly, Leu, Gly, Alagcggagatga, cgcggatgt, acgagcacca, tgacgcggtg, ttgcgctcggt, ccgcgaaggg, , , , , 720Ala, Glu, Met, Thr, Arg, Met, Tyr, Glu, His, His, Asp, Ala, Val, Leu, Arg, Ser, Val, Arg, Glu, Glygtgctggtcc, tgaccgcggg, cgggcggctg, ctggtggtca, acgacgaggc, ccgggaactg, , , , , 780Val, Leu, Val, Leu, Thr, Ala, Gly, Gly, Arg, Leu, Leu, Val, Val, Asn, Asp, Glu, Ala, Arg, Glu, Leuctcgggctgg, ctccggacgc, ggaggggcgg, cgcatcgacg, agctcggcct, cgaaccgcac, , , , , 840Leu, Gly, Leu, Ala, Pro, Asp, Ala, Glu, Gly, Arg, Arg, Ile, Asp, Glu, Leu, Gly, Leu, Glu, Pro, Hisctgacgcaac, tgctggcgtc, gggacggcgc, gtcaccgacg, aggtgcaccc, ccgcggggat, , , , , 900Leu, Thr, Gln, Leu, Leu, Ala, Ser, Gly, Arg, Arg, Val, Thr, Asp, Glu, Val, His, Pro, Arg, Gly, Aspcgactactgg, cggtcaatat, gcggtccacg, gaccgtgcgg, gcgatcccgc, cggaaacgtg, , , , , 960Arg, Leu, Leu, Ala, Val, Asn, Met, Arg, Ser, Thr, Asp, Arg, Ala, Gly, Asp, Pro, Ala, Gly, Asn, Valgtgacgctga, gggacaccac, cgcgctgcgg, gtgctgtccg, accgggccga, gcaagccggt, , , , , 1020Val, Thr, Leu, Arg, Asp, Thr, Thr, Ala, Leu, Arg, Val, Leu, Ser, Asp, Arg, Ala, Glu, Gln, Ala, Glygagcggctga, agctgctgtc, cgacgccggg, gtgcggatca, gctccagcct, ggagctgacg, , , , , 1080Glu, Arg, Leu, Lys, Leu, Leu, Ser, Asp, Ala, Gly, Val, Arg, Ile, Ser, Ser, Ser, Leu, Glu, Leu, Thrggcaccgcgg, agaagctggt, ggacgtggcc, gtcccccggt, tcgccgacat, cgtctcggtc, , , , , 1140Gly, Thr, Ala, Glu, Lys, Leu, Val, Asp, Val, Ala, Val, Pro, Arg, Phe, Ala, Asp, Ile, Val, Ser, Valgaactgctgg, agcccgtgct, gcgcggcgag, gagcccgagc, cgccgtacga, gccactggcg, , , , , 1200Glu, Leu, Leu, Glu, Pro, Val, Leu, Arg, Gly, Glu, Glu, Pro, Glu, Pro, Pro, Tyr, Glu, Pro, Leu, Alaccgcaccgga, ccgccgtcgg, cggagatccc, cccgacggcc, tcgtcttccg, cgtgggcgag, , , , , 1260Pro, His, Arg, Thr, Ala, Val, Gly, Gly, Asp, Pro, Pro, Asp, Gly, Leu, Val, Phe, Arg, Val, Gly, Glucgagtcgtct, acgcaccctc, cacaccgcag, agccgggccg, tgaaggccgg, agccgccgtc, , , , , 1320Arg, Val, Val, Tyr, Ala, Pro, Ser, Thr, Pro, Gln, Ser, Arg, Ala, Val, Lys, Ala, Gly, Ala, Ala, Valctcctgaccg, atctgacggg, ccccggcgag, tcgccgagcg, accactccgc, cccgtaccag, , , , , 1380Leu, Leu, Thr, Asp, Leu, Thr, Gly, Pro, Gly, Glu, Ser, Pro, Ser, Asp, His, Ser, Ala, Pro, Tyr, Glntcccccgggc, aatcggccac, gtacagtgcc, gagacccggc, gcctcctcga, ccgcggggtc, , , , , 1440Ser, Pro, Gly, Gln, Ser, Ala, Thr, Tyr, Ser, Ala, Glu, Thr, Arg, Arg, Leu, Leu, Asp, Arg, Gly, Valcactcgctga, tcaccgtccc, gctgcggttc, cgcggggtca, ccctcggcct, ggccaccttc, , , , , 1500His, Ser, Leu, Ile, Thr, Val, Pro, Leu, Arg, Phe, Arg, Gly, Val, Thr, Leu, Gly, Leu, Ala, Thr, Phetggcggaccc, ggcccggtga, gccgttcgac, gaggcggatc, tggcgatcgc, cggggagctg, , , , , 1560Trp, Arg, Thr, Arg, Pro, Gly, Glu, Pro, Phe, Asp, Glu, Ala, Asp, Leu, Ala, Ile, Ala, Gly, Glu, Leugccgtgcgca, ccgccgtatg, tgtcgacaac, gcccgccgct, acgcccgcga, acacaccatg, , , , , 1620Ala, Val, Arg, Thr, Ala, Val, Cys, Val, Asp, Asn, Ala, Arg, Arg, Tyr, Ala, Arg, Glu, His, Thr, Metgtcaccacct, tgcagcgcac, cctcctcccc, agcggtctgc, ccgatcagga, cgccgtgcgg, , , , , 1680Val, Thr, Thr, Leu, Gln, Arg, Thr, Leu, Leu, Pro, Ser, Gly, Leu, Pro, Asp, Gln, Asp, Ala, Val, Arggtggcgtccc, gctatctgcc, cgcacagggc, gagacgggcg, gatcctggtt, cgatgtgatc, , , , , 1740Val, Ala, Ser, Arg, Tyr, Leu, Pro, Ala, Gln, Gly, Glu, Thr, Gly, Gly, Ser, Trp, Phe, Asp, Val, Ilecctctccccg, gggcccgggt, cgcgctggtc, gtcgggaagg, tggccgggca, gggcctgcac, , , , , 1800Pro, Leu, Pro, Gly, Ala, Arg, Val, Ala, Leu, Val, Val, Gly, Lys, Val, Ala, Gly, Gln, Gly, Leu, Hisgccgcggcca, cgatggggcg, gctgcgcacc, gcggtgcaga, acttctcggc, cctggacgtg, , , , , 1860Ala, Ala, Ala, Thr, Met, Gly, Arg, Leu, Arg, Thr, Ala, Val, Gln, Asn, Phe, Ser, Ala, Leu, Asp, Valcccccggatg, agctcctctc, ccatctggac, gagctggtca, cccgtctcga, cctggagcgc, , , , , 1920Pro, Pro, Asp, Glu, Leu, Leu, Ser, His, Leu, Asp, Glu, Leu, Val, Thr, Arg, Leu, Asp, Leu, Glu, Arggaggccgatt, cggacgacgt, ccggatcacg, ggcgccacct, gcctgtacgc, gatccacgac, , , , , 1980Glu, Ala, Asp, Ser, Asp, Asp, Val, Arg, Ile, Thr, Gly, Ala, Thr, Cys, Leu, Tyr, Ala, Ile, His, Asptcggtgtccg, gccactgcgc, catggcccgg, gccggcgatc, cgggcatcgc, cgtgacccac, , , , , 2040Ser, Val, Ser, Gly, His, Cys, Ala, Met, Ala, Arg, Ala, Gly, Asp, Pro, Gly, Ile, Ala, Val, Thr, Hisccggacggca, ccgtggacct, ccctgcggta, cccatcggcc, cggccctggg, catgggcggg, , , , , 2100Pro, Asp, Gly, Thr, Val, Asp, Leu, Pro, Ala, Val, Pro, Ile, Gly, Pro, Ala, Leu, Gly, Met, Gly, Glygagccgttcg, aggcggtcgg, cctctcgctg, cccgccgcaa, gccggctggt, gctgtacacc, , , , , 2160Glu, Pro, Phe, Glu, Ala, Val, Gly, Leu, Ser, Leu, Pro, Ala, Ala, Ser, Arg, Leu, Val, Leu, Tyr, Thraacggccttc, ttgaagggga, aggccaagcc, gccgacaccg, gcctcgacct, gctgcgccgc, , , , , 2220Asn, Gly, Leu, Leu, Glu, Gly, Glu, Gly, Gln, Ala, Ala, Asp, Thr, Gly, Leu, Asp, Leu, Leu, Arg, Argaccctcgcgg, ccgagccgga, cctcggcccg, gacgagacct, gccggagcct, tttcgacacc, , , , , 2280Thr, Leu, Ala, Ala, Glu, Pro, Asp, Leu, Gly, Pro, Asp, Glu, Thr, Cys, Arg, Ser, Leu, Phe, Asp, Thrgtgcttccgg, cccacccgag, cgacgatgtg, gcgctgctgg, tggcccggac, ccgcctgctc, , , , , 2340Val, Leu, Pro, Ala, His, Pro, Ser, Asp, Asp, Val, Ala, Leu, Leu, Val, Ala, Arg, Thr, Arg, Leu, Leugccccggaga, acgtggccga, gtgggatgt, gccgttcgacc, tggcggcggt, cgccccgctg, , , , , 2400Ala, Pro, Glu, Asn, Val, Ala, Glu, Trp, Asp, Val, Pro, Phe, Asp, Leu, Ala, Ala, Val, Ala, Pro, Leucgcgccacct, gcacccggaa, actgcgggcg, tggggcctgg, aggacgccgc, gtacaccgcc, , , , , 2460Arg, Ala, Thr, Cys, Thr, Arg, Lys, Leu, Arg, Ala, Trp, Gly, Leu, Glu, Asp, Ala, Ala, Tyr, Thr, Alagagctgatca, tcagtgaact, gatcaccaa, cgccctgcgg, tacggctcccc, tcccgtacgc, , , , , 2520Glu, Leu, Ile, Ile, Ser, Glu, Leu, Ile, Thr, Asn, Ala, Leu, Arg, Tyr, Gly, Ser, Pro, Pro, Val, Argatacggctgc, tgcgcggccg, cggcctgatc, ttcgaggtct, ccgacggcag, cagcaccgca, , , , , 2580Ile, Arg, Leu, Leu, Arg, Gly, Arg, Gly, Leu, Ile, Phe, Glu, Val, Ser, Asp, Gly, Ser, Ser, Thr, Alaccccatctgc, ggcgggccgc, gatcaccgac, gagggcggcc, gcgggctgtt, cctcgtcgcc, , , , , 2640Pro, His, Leu, Arg, Arg, Ala, Ala, Ile, Thr, Asp, Glu, Gly, Gly, Arg, Gly, Leu, Phe, Leu, Val, Alacagttcgccc, agcgctgggg, cacccgctac, accccgcacg, gcaaggtcat, ctgggccgag, , , , , 2700Gln, Phe, Ala, Gln, Arg, Trp, Gly, Thr, Arg, Tyr, Thr, Pro, His, Gly, Lys, Val, Ile, Trp, Ala, Glugcggccctgg, acggcggcct, c, , , , , , , , , , , , , , , , 2721Ala, Ala, Leu, Asp, Gly, Gly, the Leu gene outcomes and Streptomyces, coelicolorA3, (, 2, ), the film sensor of middle hypothesis, (, IMSP, ), there is good homology, (, 51%, ),,Similitude (63%). Analysis of amino acid sequence, shown in the presence of the homology analysis of 3 conserved domains (delta antigen-PP2C phosphatases, K+ passages, cGMP phosphodiesterases) conserved domain, with delta antigen regulatory protein phosphatases seemingly, explanation may be relevant with transcriptional regulatory for the gene outcome.orf5:561 VASRYLPAQGETGGSWFDVIPLPGARVALVVGKVAGQGLHAAATMGRLRTAVQNFSALDV 620PP2C_sig:6 IAQYYEDAT-QVGGDYYDVVALPEGRLLIAIADVMGKGLAAALAMGMARSALRTLLSEGI 64orf5:621 PPDELLSHLDELVTRLDLEREADSDDVRITGATCLYAIHDSVSGHCAMARAGDPGIAVTH 680PP2C_sig:65 SLSQILERLNRAIYENEEDG--------MFATLFLALYDFATGTLSYANAGHSPPYLLR 115orf5:681 PDGTVDLPAVPIGPALGMGGE-PFEAVGLSLPAASRLVLYTNGLLEGEGQAADTGLDLLR 739PP2C_sig:116 ADGGLVEILTDLGAPLGLEPDVEVDVRELTLEPGDLLLLYTDGLT--EARSPFFGEERLE 173orf5:740 RTLAAEPDLGPDETCRSLFDTVL---PAHPSDD 769PP2C_sig:174 ELLEALLGSAPQEIAQEILAELLEFAGGRGEDD 206orf5:228 EHHDAVLRSVREGVLVLTAGGRLLVVNDEARELLGLAP-DAEGRRIDELGLEPHLTQLLA 286K+:1 ERLRAILESLPDGVFVLDLDGRILYANPAAEELLGYSPEELIGKSLLELIHPEDREELQE 60orf5:287 SGRR 290K+:61 RLQR 64orf5:478 RGVHSLITVPLRFRGVTLGLATFWRTRPGEPFDEADLAIAGELAVRTAVCVDNARRYARE 537cGMP:89 QLIRSFLAVPLVAGGELLGVLALHRKDSPRPFTEEEEELLQALANQLAIALALAQLYEEL 148orf5:538 H 538cGMP:149 R 14919.orf6 (Genbank indexed number AF521895):Proteolysis enzyme gene, size is 969bpDNA, encodes the albumen being made up of 323 amino acid.Gtgagaacag, ctcgccgtac, caggagacgt, ggccggttga, ccgccgcggt, gtccggtctg, , , , , 60Val, Arg, Thr, Ala, Arg, Arg, Thr, Arg, Arg, Arg, Gly, Arg, Leu, Thr, Ala, Ala, Val, Ser, Gly, Leuttcatcacgg, cagccctcgc, gacagtcggt, acgagtgcgg, ccgcttcctc, cgcgagacc, , , , , 120Phe, Ile, Thr, Ala, Ala, Leu, Ala, Thr, Val, Gly, Thr, Ser, Ala, Ala, Ala, Ser, Ser, Ala, Met, Thrgccacgtccg, cgcccagtgc, caccgccacg, cccccgtccg, tatccacgcc, cgtgtccggc, , , , , 180Ala, Thr, Ser, Ala, Pro, Ser, Ala, Thr, Ala, Thr, Pro, Pro, Ser, Val, Ser, Thr, Pro, Val, Ser, Glygccgccacgc, ccgtgtccgg, cgccgcctcg, tccgtgtccg, gcgccgcctc, ggctgtggca, , , , , 240Ala, Ala, Thr, Pro, Val, Ser, Gly, Ala, Ala, Ser, Ser, Val, Ser, Gly, Ala, Ala, Ser, Ala, Val, Alatccctggatg, tcccgggcac, cgcctggacc, gtggacgagc, gcaccggaac, gctgcgagtc, , , , , 300Ser, Leu, Asp, Val, Pro, Gly, Thr, Ala, Trp, Thr, Val, Asp, Glu, Arg, Thr, Gly, Thr, Leu, Arg, Valctcgtcggtt, ccacggcccg, ggaagccgat, ctggccaggc, tcgaccgcac, cgccgagcgc, , , , , 360Leu, Val, Gly, Ser, Thr, Ala, Arg, Glu, Ala, Asp, Leu, Ala, Arg, Leu, Asp, Arg, Thr, Ala, Glu, Argttcggcggca, cgatcaccgt, ggagcggctc, gacggtccgc, tgcggaccct, gctctccggt, , , , , 420Phe, Gly, Gly, Thr, Ile, Thr, Val, Glu, Arg, Leu, Asp, Gly, Pro, Leu, Arg, Thr, Leu, Leu, Ser, Glyggcgacggga, tccactccac, cacggggctg, cgctgctccg, cgggggtcaa, tgtgcaaagc, , , , , 480Gly, Asp, Gly, Ile, His, Ser, Thr, Thr, Gly, Leu, Arg, Cys, Ser, Ala, Gly, Val, Asn, Val, Gln, Serggcaccacgt, attacttcgt, cacggccggc, cactgcaccg, acgccgcccc, cacctggtac, , , , , 540Gly, Thr, Thr, Tyr, Tyr, Phe, Val, Thr, Ala, Gly, His, Cys, Thr, Asp, Ala, Ala, Pro, Thr, Trp, Tyraccggctccg, atgcgaccac, cccggtcggt, tcgacgaccg, ccaccagctt, cccgggcaat, , , , , 600Thr, Gly, Ser, Asp, Ala, Thr, Thr, Pro, Val, Gly, Ser, Thr, Thr, Ala, Thr, Ser, Phe, Pro, Gly, Asngactacggcg, tcgtccggta, caccaacacg, gccgttccgc, accccgggac, cgtgggaacc, , , , , 660Asp, Tyr, Gly, Val, Val, Arg, Tyr, Thr, Asn, Thr, Ala, Val, Pro, His, Pro, Gly, Thr, Val, Gly, Thrgtggacatca, ccgggaccgc, caccgcctac, gtcggccagc, aggtctgccg, ccggggtgcc, , , , , 720Val, Asp, Ile, Thr, Gly, Thr, Ala, Thr, Ala, Tyr, Val, Gly, Gln, Gln, Val, Cys, Arg, Arg, Gly, Alaacgaccggcg, tccggtgcgg, tcaggtcatc, gcgctcaacg, ccaccgtcaa, ctacggcggc, , , , , 780Thr, Thr, Gly, Val, Arg, Cys, Gly, Gln, Val, Ile, Ala, Leu, Asn, Ala, Thr, Val, Asn, Tyr, Gly, Glyggtgatgtcg, tctccggcct, gatccagacc, aatatctgcg, ccgagccggg, cgacagcggc, , , , , 840Gly, Asp, Val, Val, Ser, Gly, Leu, Ile, Gln, Thr, Asn, Ile, Cys, Ala, Glu, Pro, Gly, Asp, Ser, Glyggtccgctct, acgcgggcga, caagatcatc, ggcattctct, cgggcggctc, cggggactgc, , , , , 900Gly, Pro, Leu, Tyr, Ala, Gly, Asp, Lys, Ile, Ile, Gly, Ile, Leu, Ser, Gly, Gly, Ser, Gly, Asp, Cysgcgaccggag, gcaccacctt, ctaccagccg, atccaggagg, tgctgagcgc, ctacggcctc, , , , , 960Ala, Thr, Gly, Gly, Thr, Thr, Phe, Tyr, Gln, Pro, Ile, Gln, Glu, Val, Leu, Ser, Ala, Tyr, Gly, Leuaccgtctac, 969Thr, Val, the Tyr gene outcomes show that the Cathepsin B precursor SGPB with streptomyces griseus has good homology, (, 58%, ),,Similitude (74%). SGPB belongs to peptidase families, is also the family of crack protein enzyme, there is proteolytic enzyme activity, and the enzyme has the specificity of first choice to big aliphatic amino acid or aromatic amino acid.The arrangement hoc scenario of 19 geldanamycin biosynthesis gene encoder blocks of the above is shown in Figure 62 0.gdnC (Genbank AF521896):PKS genes, size is 1659bpDNA, encodes the albumen being made up of 553 amino acid.Gtggcaccaa, cgccccgtca, tctggaacag, gcggccccga, cggcgaccga, gtcggccgat, , , , , 60Val, Ala, Pro, Thr, Pro, Arg, His, Leu, Glu, Gln, Ala, Ala, Pro, Thr, Ala, Thr, Glu, Ser, Ala, Aspccggcgctgt, cctggccgaa, gggcgtccct, gtgccgctgg, tggtgtccgg, ccgaggcgcc, , , , , 120Pro, Ala, Leu, Ser, Trp, Pro, Lys, Gly, Val, Pro, Val, Pro, Leu, Val, Val, Ser, Gly, Arg, Gly, Alagcggcgctcg, ccgcccaggc, gcaacggcta, cggaccttcg, tagccgacga, gccgcaactc, , , , , 180Ala, Ala, Leu, Ala, Ala, Gln, Ala, Gln, Arg, Leu, Arg, Thr, Phe, Val, Ala, Asp, Glu, Pro, Gln, Leugacttgagcg, aactcggcta, cgcgttgggt, tgtggtcggg, cggggttgtc, ggatcgtggg, , , , , 240Asp, Leu, Ser, Glu, Leu, Gly, Tyr, Ala, Leu, Gly, Cys, Gly, Arg, Ala, Gly, Leu, Ser, Asp, Arg, Glygtggtggtgg, cgggtggtcg, tgaggagttg, ttggtggggt, tgggtgggtt, ggtgcggggt, , , , , 300Val, Val, Val, Ala, Gly, Gly, Arg, Glu, Glu, Leu, Leu, Val, Gly, Leu, Gly, Gly, Leu, Val, Arg, Glygaggggggtg, tgggtgtggt, gtcgggttcg, gtggtgcgtg, gtcggttggg, ggtgttgttt, , , , , 360Glu, Gly, Gly, Val, Gly, Val, Val, Ser, Gly, Ser, Val, Val, Arg, Gly, Arg, Leu, Gly, Val, Leu, Phegctggtcagg, ggtgtcagcg, ggtggggatg, gggcgtggg, ttgtatgaggt, gttcccggtg, , , , , 420Ala, Gly, Gln, Gly, Cys, Gln, Arg, Val, Gly, Met, Gly, Arg, Gly, Leu, Tyr, Glu, Val, Phe, Pro, Valttccgggatg, ccttcgacgc, ggtgtgtgag, gtgttggatc, gggagttggg, tgcgggtggt, , , , , 480Phe, Arg, Asp, Ala, Phe, Asp, Ala, Val, Cys, Glu, Val, Leu, Asp, Arg, Glu, Leu, Gly, Ala, Gly, Glygtggtgggtt, cggtgcggga, ggtggtgttc, gggggtgggg, ggttgttgga, gcggacggtg, , , , , 540Val, Val, Gly, Ser, Val, Arg, Glu, Val, Val, Phe, Gly, Gly, Gly, Gly, Leu, Leu, Glu, Arg, Thr, Valtttgctcagg, cggggttgtt, cgccgtggag, gtggggttgt, tccggttggt, ggagtcgtgg, , , , , 600Phe, Ala, Gln, Ala, Gly, Leu, Phe, Ala, Val, Glu, Val, Gly, Leu, Phe, Arg, Leu, Val, Glu, Ser, Trpggtgtggtgg, tggatgtggt, gggtgggcat, tcggtgggtg, aggtgacggc, ggcgtatgtg, , , , , 660Gly, Val, Val, Val, Asp, Val, Val, Gly, Gly, His, Ser, Val, Gly, Glu, Val, Thr, Ala, Ala, Tyr, Valgcgggtgtgt, tgtcgttgga, ggatgcggcg, gtgttggtgg, cggcgcgggg, tcggttgatg, , , , , 720Ala, Gly, Val, Leu, Ser, Leu, Glu, Asp, Ala, Ala, Val, Leu, Val, Ala, Ala, Arg, Gly, Arg, Leu, Metgaggcgttgc, cggagggtgg, ggcgatggtg, gcggtggctg, cgggtgagga, ggtggtgcgg, , , , , 780Glu, Ala, Leu, Pro, Glu, Gly, Gly, Ala, Met, Val, Ala, Val, Ala, Ala, Gly, Glu, Glu, Val, Val, Argcctttgctgg, tgtcggcggt, ggatattgcg, gcggtgaacg, ggcccgaagc, ggtggtgctc, , , , , 840Pro, Leu, Leu, Val, Ser, Ala, Val, Asp, Ile, Ala, Ala, Val, Asn, Gly, Pro, Glu, Ala, Val, Val, Leutccggtgatg, aggagccggt, actacgggtt, gcgcgcgatt, tgtcggatca, ggggtgtcgg, , , , , 900Ser, Gly, Asp, Glu, Glu, Pro, Val, Leu, Arg, Val, Ala, Arg, Asp, Leu, Ser, Asp, Gln, Gly, Cys, Argacgaggcgtt, tggcggtttc, gcatgcgttc, cattccgccc, gtatggagcc, gatgctggag, , , , , 960Thr, Arg, Arg, Leu, Ala, Val, Ser, His, Ala, Phe, His, Ser, Ala, Arg, Met, Glu, Pro, Met, Leu, Glugagttccggg, aggcgatcgc, cgatctgtcg, ttctcggcgc, cggtgattcc, tctggtgtcg, , , , , 1020Glu, Phe, Arg, Glu, Ala, Ile, Ala, Asp, Leu, Ser, Phe, Ser, Ala, Pro, Val, Ile, Pro, Leu, Val, Seraatgtgaccg, ggcggttggc, ggatgcggag, accgtgtgtt, cgccggagta, ctgggtggag, , , , , 1080Ash, Val, Thr, Gly, Arg, Leu, Ala, Asp, Ala, Glu, Thr, Val, Cys, Ser, Pro, Glu, Tyr, Trp, Val, Glucatgtgcgtt, cggccgtgcg, gttcgcggac, ggtgtgcggg, cgctcgctga, ctacggtgtg, , , , , 1140His, Val, Arg, Ser, Ala, Val, Arg, Phe, Ala, Asp, Gly, Val, Arg, Ala, Leu, Ala, Asp, Tyr, Gly, Valggcacctatc, tggagttggc, gccggatgcg, gtgttgtccg, cgatggttgg, tgattgtcta, , , , , 1200Gly, Thr, Tyr, Leu, Glu, Leu, Ala, Pro, Asp, Ala, Val, Leu, Ser, Ala, Met, Val, Gly, Asp, Cys, Leuccggaagggt, cggctgctga, gagtgtggtg, gtgccgtcgc, tgcggcggga, gggcgacgag, , , , , 1260Pro, Glu, Gly, Ser, Ala, Ala, Glu, Ser, Val, Val, Val, Pro, Ser, Leu, Arg, Arg, Glu, Gly, Asp, Gluccccgtgcgc, tgatgaccgc, catcgctcag, ctgcatgtgg, caggcgtacc, catcgacttc, , , , , 1320Pro, Arg, Ala, Leu, Met, Thr, Ala, Ile, Ala, Gln, Leu, His, Val, Ala, Gly, Val, Pro, Ile, Asp, Pheggtgccctgt, tcggtgccac, ggttctgccc, acccatattt, cggctctgcc, gacgtatgcg, , , , , 1380Gly, Ala, Leu, Phe, Gly, Ala, Thr, Val, Leu, Pro, Thr, His, Ile, Ser, Ala, Leu, Pro, Thr, Tyr, Alattccagcggg, agcattactg, gttggtgggg, gacgggcgtg, gagccggcga, tgtggcgtcc, , , , , 1440Phe, Gln, Arg, Glu, His, Tyr, Trp, Leu, Val, Gly, Asp, Gly, Arg, Gly, Ala, Gly, Asp, Val, Ala, Sergccgggctgg, cgggggtgga, gcatccattc, ctgggcgcga, tgacggaggt, gcccgggtcg, , , , , 1500Ala, Gly, Leu, Ala, Gly, Val, Glu, His, Pro, Phe, Leu, Gly, Ala, Met, Thr, Glu, Val, Pro, Gly, Serggtgaggtgt, tgttctcctc, gcggttgtcg, ttggggtctca, tccgtggct, ggccgatcat, , , , , 1560Gly, Glu, Val, Leu, Phe, Ser, Ser, Arg, Leu, Ser, Leu, Gly, Ser, His, Pro, Trp, Leu, Ala, Asp, Hisgtggctgcgg, gtgcggtgtt, gttgccgggt, gcggcgtttg, tggagttggt, ggtgcggagc, , , , , 1620Val, Ala, Ala, Gly, Ala, Val, Leu, Leu, Pro, Gly, Ala, Ala, Phe, Val, Glu, Leu, Val, Val, Arg, Sertggacgatga, ggtgggctgc, ggtggggtgg, aggagctgg, 1659Trp, Thr, Met, Arg, Trp, Ala, Ala, Val, Gly, Trp, Arg, Ser, the Trp genes and rifamycin, rif9 I types PKS is homologous, (, 41%, ),,Similitude (51%).gdnC.pro ------------------------------------------------------------ 1rif9.pro MATDEKLLKYLKRVTAELHSLRKQGARHADEPLAVVGMACRFPGGVSSPEDLWQLVAGGV 60gdnC.pro ------------------------------------------------------------ 1rif9.pro DALSDFPDDRGWELDGLFDPDPDHPGTSYTSQGGFLRGAGLFDAGLFGISPREALVMDPQ 120gdnC.pro ------------------------------------------------------------ 1rif9.pro QRVLLETSWEALEDAGVDPLSLKGSDVGVFSGVFTQGYGAGAITPDLEAFAGIGAASSVA 180gdnC.pro ------------------------------------------------------------ 1rifg.pro SGRVSYVFGLEGPAVTIDTACSSSLVAIHLAAQALRAGECSMALAGGATVMPTPGTFVAF 240gdnC.pro ------------------------------------------------------------ 1rif9.pro SRQRVLAADGRSKAFSSTADGTGWAEGAGVLVLERLSVAQERGHRILAVLRGSAVNQDGA 300gdnC.pro ------------------------------------------------------------ 1rif9.pro SNGLTAPNGPSQQRVIRKALAGAGLVASDVDVVEAHGTGTALGDPIEAQALLATYGQGRE 360 Amino acid sequence analysis discovery contains a conserved domain (see Fig. 7) in amino acid/11 20-421 positions, and the domain is acyltransferase domain (AT), encodes malonyl transferase, MT.21.gdnD(Genbank AF521896):PKS genes, are an incomplete ORF, and size is 3339bpDNA, encode 1113 amino acid.Gtgggcgccg, taccgctcca, ggaaccgctc, ggcgtaggcg, gggccgaacc, aggggtcctc, , , , , 60Val, Gly, Ala, Val, Pro, Leu, Gln, Glu, Pro, Leu, Gly, Val, Gly, Gly, Ala, Glu, Pro, Gly, Val, Leugtcgatctcc, agctcgcggg, cggccagggc, gatgtcggcg, tggcggtcgg, cgaccccgag, , , , , 120Val, Asp, Leu, Gln, Leu, Ala, Gly, Gly, Gln, Gly, Asp, Val, Gly, Val, Ala, Val, Gly, Asp, Pro, Glugcggccgacg, tcgatcacgc, cggtgacccg, gcaggtcccg, gggtcgagca, ggacgttgtt, , , , , 180Ala, Ala, Asp, Val, Asp, His, Ala, Gly, Asp, Pro, Ala, Gly, Pro, Gly, Val, Glu, Gln, Asp, Val, Valggggcacagg, tcgccatggc, agacgaccag, gtcctccttc, tcgggacggg, tgcggtcgag, , , , , 240Gly, Ala, Gln, Val, Ala, Met, Ala, Asp, Asp, Gln, Val, Leu, Leu, Leu, Gly, Thr, Gly, Ala, Val, Gluctccgccagg, agctggtcgc, cggtccaccc, ggcccgctcc, tcctgcaggt, cgtcgaggtc, , , , , 300Leu, Arg, Gln, Glu, Leu, Val, Ala, Gly, Pro, Pro, Gly, Pro, Leu, Leu, Leu, Gln, Val, Val, Glu, Valcaccaggccc, tcggcgacgt, tccgccgggc, ctcggcgacc, gccgcgtcga, ggcgccggtc, , , , , 360His, Gln, Ala, Leu, Gly, Asp, Val, Pro, Pro, Gly, Leu, Gly, Asp, Arg, Arg, Val, Glu, Ala, Pro, Valgaaggggcag, tcctccacgg, gcagctcgtg, gagggcgcgg, gccagctccg, ccatcgcctc, , , , , 420Glu, Gly, Ala, Val, Leu, His, Gly, Gln, Leu, Val, Glu, Gly, Ala, Gly, Gln, Leu, Arg, His, Arg, Leugaccacggcg, aaccgctggt, gctcgggcca, ctcctcggcc, gccgcgacgc, cggggacggc, , , , , 480Asp, His, Gly, Glu, Pro, Leu, Val, Leu, Gly, Pro, Leu, Leu, Gly, Arg, Arg, Asp, Ala, Gly, Asp, Glyctccgtgacg, agccacgcgg, cggtgtcgtc, ggcaccgcgc, tcgacgacgc, gggggacggg, , , , , 540Leu, Arg, Asp, Glu, Pro, Arg, Gly, Gly, Val, Val, Gly, Thr, Ala, Leu, Asp, Asp, Ala, Gly, Asp, Glygatcgggtgg, atgtggtgca, gccggtgtcg, tttgcggtga, tggtggggtt, ggcgcgggtg, , , , , 600Asp, Arg, Val, Asp, Val, Val, Gln, Pro, Val, Ser, Phe, Ala, Val, Met, Val, Gly, Leu, Ala, Arg, Valtggttggcgg, ctggtgtggt, gccgtcggtg, gtggtgggtc, attcgcaggg, ggagattgcg, , , , , 660Trp, Leu, Ala, Ala, Gly, Val, Val, Pro, Ser, Val, Val, Val, Gly, His, Ser, Gln, Gly, Glu, Ile, Alagctgcgtgtg, tggcgggtgg, gttgtcgttg, gaggacgcgg, tgcgggtggt, ggtgttgcgg, , , , , 720Ala, Ala, Cys, Val, Ala, Gly, Gly, Leu, Ser, Leu, Glu, Asp, Ala, Val, Arg, Val, Val, Val, Leu, Argagtcgtgcgg, tggcggctgg, gctctcgggc, cgtggcggga, tggtgtcgtt, ggcggtgggt, , , , , 780Ser, Arg, Ala, Val, Ala, Ala, Gly, Leu, Ser, Gly, Arg, Gly, Gly, Met, Val, Ser, Leu, Ala, Val, Glygtggcggagg, cggaggggtt, ggttgagcgg, tggtcggggc, gtatcgaggt, ggcggcggtg, , , , , 840Val, Ala, Glu, Ala, Glu, Gly, Leu, Val, Glu, Arg, Trp, Ser, Gly, Arg, Ile, Glu, Val, Ala, Ala, Valaatgggccgt, tgtcggtggt, ggtggctggt, gagccggatg, ccttgcgggg, gttggtggcg, , , , , 900Asn, Gly, Pro, Leu, Ser, Val, Val, Val, Ala, Gly, Glu, Pro, Asp, Ala, Leu, Arg, Gly, Leu, Val, Alagagtgtgagg, gcgcgggggt, gcgggcgcgg, tgggttgatg, tggattacgc, ctcgcatacg, , , , , 960Glu, Cys, Glu, Gly, Ala, Gly, Val, Arg, Ala, Arg, Trp, Val, Asp, Val, Asp, Tyr, Ala, Ser, His, Thrgcgcaggtgg, aggcggtcga, gggggagttg, gctcggtcgt, tggcgcaaat, tcgtccggtg, , , , , 1020Ala, Gln, Val, Glu, Ala, Val, Glu, Gly, Glu, Leu, Ala, Arg, Ser, Leu, Ala, Gln, Ile, Arg, Pro, Valtcctcacgta, ttccgttctt, ttcgacggtg, gaggctgggt, ggctggacac, ggccgagctg, , , , , 1080Ser, Ser, Arg, Ile, Pro, Phe, Phe, Ser, Thr, Val, Glu, Ala, Gly, Trp, Leu, Asp, Thr, Ala, Glu, Leugacgccgggt, actggtaccg, gaatctgcgg, agcaccgtgc, gcttcgcgcc, gtcgatcgac, , , , , 1140Asp, Ala, Gly, Tyr, Trp, Tyr, Arg, Asn, Leu, Arg, Ser, Thr, Val, Arg, Phe, Ala, Pro, Ser, Ile, Aspcggttgatcg, aggaaggctt, tgcggcgttt, gtcgaagtga, gcgcgcatcc, ggtgctgacg, , , , , 1200Arg, Leu, Ile, Glu, Glu, Gly, Phe, Ala, Ala, Phe, Val, Glu, Val, Ser, Ala, His, Pro, Val, Leu, Thratgggcatcg, aggcggcggc, ggagcgggcg, gacgttgggc, cggtcgtggt, gaccgggacg, , , , , 1260Met, Gly, Ile, Glu, Ala, Ala, Ala, Glu, Arg, Ala, Asp, Val, Gly, Pro, Val, Val, Val, Thr, Gly, Thrctccgccggg, atcagggtga, tatgcgtcgt, gtgctcactt, ccctggccga, ggtgtacgta, , , , , 1320Leu, Arg, Arg, Asp, Gln, Gly, Asp, Met, Arg, Arg, Val, Leu, Thr, Ser, Leu, Ala, Glu, Val, Tyr, Valcgcggtgtcc, ccgtgaactg, gaccaccctg, ctgggcgaca, tcccggcgcg, cgccgcgttg, , , , , 1380Arg, Gly, Val, Pro, Val, Asn, Trp, Thr, Thr, Leu, Leu, Gly, Asp, Ile, Pro, Ala, Arg, Ala, Ala, Leugatctgccga, cgtacgcctt, ccagcatcag, cactactggc, tgaagaacgc, cattcccacc, , , , , 1440Asp, Leu, Pro, Thr, Tyr, Ala, Phe, Gln, His, Gln, His, Tyr, Trp, Leu, Lys, Asn, Ala, Ile, Pro, Thrgatgcgggag, ccatcgacga, tcagcttccg, ggcctggtcg, aactgcccgc, cgagaccggc, , , , , 1500Asp, Ala, Gly, Ala, Ile, Asp, Asp, Gln, Leu, Pro, Gly, Leu, Val, Glu, Leu, Pro, Ala, Glu, Thr, Glygccttgaccg, ctcgccttct, tggggagtcc, acgcaggaac, aggaacgcat, cctgctcaag, , , , , 1560Ala, Leu, Thr, Ala, Arg, Leu, Leu, Gly, Glu, Ser, Thr, Gln, Glu, Gln, Glu, Arg, Ile, Leu, Leu, Lysaccgttcgcc, aggagaccgc, gagcgtcttg, ggccactcct, cgctggacgc, cattgaaccg, , , , , 1620Thr, Val, Arg, Gln, Glu, Thr, Ala, Ser, Val, Leu, Gly, His, Ser, Ser, Leu, Asp, Ala, Ile, Glu, Progacatggtgt, tcaaccagat, cggcttcgac, tcggccaccg, cagtacagct, gcgaaaccgt, , , , , 1680Asp, Met, Val, Phe, Asn, Gln, Ile, Gly, Phe, Asp, Ser, Ala, Thr, Ala, Val, Gln, Leu, Arg, Asn, Argctgaacgcgc, tcaccgaccg, gactctgccg, accaccctgc, tcttcgacta, ccccacgccc, , , , , 1740Leu, Asn, Ala, Leu, Thr, Asp, Arg, Thr, Leu, Pro, Thr, Thr, Leu, Leu, Phe, Asp, Tyr, Pro, Thr, Proctgatcctcg, ccgacttcct, gcgtgacgaa, ctcatcgggg, acacggcggc, cccggagggg, , , , , 1800Leu, Ile, Leu, Ala, Asp, Phe, Leu, Arg, Asp, Glu, Leu, Ile, Gly, Asp, Thr, Ala, Ala, Pro, Glu, Glygtgccggaag, cgacagcggc, gccgggggat, gtgtcgaccg, agccggtggc, gatcgtgggt, , , , , 1860Val, Pro, Glu, Ala, Thr, Ala, Ala, Pro, Gly, Asp, Val, Ser, Thr, Glu, Pro, Val, Ala, Ile, Val, Glyatggcgtgcc, ggctgccggg, tggcgtctcc, accccggaag, agctatggga, cctggtgctt, , , , , 1920Met, Ala, Cys, Arg, Leu, Pro, Gly, Gly, Val, Ser, Thr, Pro, Glu, Glu, Leu, Trp, Asp, Leu, Val, Leucaggggcggg, acggggtcag, cgacttcccc, gtgaaccgtg, gctgggatct, ggagaatctg, , , , , 1980Gln, Gly, Arg, Asp, Gly, Val, Ser, Asp, Phe, Pro, Val, Asn, Arg, Gly, Trp, Asp, Leu, Glu, Asn, Leuttccacccgg, acccggacca, ccccgctacc, agctatgcgc, accaaggcgg, atttctgcac, , , , , 2040Phe, His, Pro, Asp, Pro, Asp, His, Pro, Ala, Thr, Ser, Tyr, Ala, His, Gln, Gly, Gly, Phe, Leu, Hisgacgccgggg, agtttgacgc, gggtttcttc, gggatctcac, cacgcgaggc, actggccgtg, , , , , 2100Asp, Ala, Gly, Glu, Phe, Asp, Ala, Gly, Phe, Phe, Gly, Ile, Ser, Pro, Arg, Glu, Ala, Leu, Ala, Valgacccgcaac, agcgtctgat, gctggaaacc, tcgtgggaag, cgctggaacg, cgccgggatc, , , , , 2160Asp, Pro, Gln, Gln, Arg, Leu, Met, Leu, Glu, Thr, Ser, Trp, Glu, Ala, Leu, Glu, Arg, Ala, Gly, Ilegacccgacca, cgctgcgggg, caaggacgtc, ggtgtcttct, ccggtgtgac, gtaccacaac, , , , , 2220Asp, Pro, Thr, Thr, Leu, Arg, Gly, Lys, Asp, Val, Gly, Val, Phe, Ser, Gly, Val, Thr, Tyr, His, Asntacggctcgg, gcgtggagcc, ggttcccgcc, gagctcgaag, gcatgctggg, gctcggcgcc, , , , , 2280Tyr, Gly, Ser, Gly, Val, Glu, Pro, Val, Pro, Ala, Glu, Leu, Glu, Gly, Met, Leu, Gly, Leu, Gly, Alatcggcgagcg, tgctgtcagg, gcgggtgtcg, tatgcgctgg, gcttcgaggg, gccgtcggtc, , , , , 2340Ser, Ala, Ser, Val, Leu, Ser, Gly, Arg, Val, Ser, Tyr, Ala, Leu, Gly, Phe, Glu, Gly, Pro, Ser, Valgcggtggaca, cggcgtgctc, ctcgtccctg, gtggcgttgc, acttggcggc, gcaggcgttg, , , , , 2400Ala, Val, Asp, Thr, Ala, Cys, Ser, Ser, Ser, Leu, Val, Ala, Leu, His, Leu, Ala, Ala, Gln, Ala, Leucgagcaggcg, agtgctcgat, cgcccttgcc, ggtggggtc, acggtgatgcc, gactcccggt, , , , , 2460Arg, Ala, Gly, Glu, Cys, Ser, Ile, Ala, Leu, Ala, Gly, Gly, Val, Thr, Val, Met, Pro, Thr, Pro, Glyatcttcatcg, ccttctcacg, gcagcgcggc, atgtcggtcg, atggccggtg, caagtcgttc, , , , , 2520Ile, Phe, Ile, Ala, Phe, Ser, Arg, Gln, Arg, Gly, Met, Ser, Val, Asp, Gly, Arg, Cys, Lys, Ser, Phetcggcgtcgg, cggacggtac, ggggtgggcc, gagggtgtgg, gtgtgctggc, gctggagcgg, , , , , 2580Ser, Ala, Ser, Ala, Asp, Gly, Thr, Gly, Trp, Ala, Glu, Gly, Val, Gly, Val, Leu, Ala, Leu, Glu, Argctgtcggacg, cggagcgaaa, cggccatcgg, gtgttggcgg, tggtgcgggg, cagtgcggtg, , , , , 2640Leu, Ser, Asp, Ala, Glu, Arg, Asn, Gly, His, Arg, Val, Leu, Ala, Val, Val, Arg, Gly, Ser, Ala, Valaatcaggacg, gtgcgtcgaa, tgggttgacg, gcgccgaatg, gtccgtcgca, gcagcgtgtc, , , , , 2700Asn, Gln, Asp, Gly, Ala, Ser, Asn, Gly, Leu, Thr, Ala, Pro, Asn, Gly, Pro, Ser, Gln, Gln, Arg, Valattcggcagg, cgctggccag, tgcgggtgtg, tcggctgccg, aggtggatgt, ggtcgaggca, , , , , 2760Ile, Arg, Gln, Ala, Leu, Ala, Ser, Ala, Gly, Val, Ser, Ala, Ala, Glu, Val, Asp, Val, Val, Glu, Alacatggcacgg, gtacggcgct, gggcgatccc, attgaggcgc, aggcggtgt, tggccacgtat, , , , , 2820His, Gly, Thr, Gly, Thr, Ala, Leu, Gly, Asp, Pro, Ile, Glu, Ala, Gln, Ala, Val, Leu, Ala, Thr, Tyrggccaggatc, gtgatcggcc, tttgttgatg, gggtcgttga, agtcgaatat, cggtcatgcg, , , , , 2880Gly, Gln, Asp, Arg, Asp, Arg, Pro, Leu, Leu, Met, Gly, Ser, Leu, Lys, Ser, Asn, Ile, Gly, His, Alacaggcggccg, cgggtgtggc, tggtgtgatc, aagatggtgt, tggcgctgcg, gcatggcatc, , , , , 2940Gln, Ala, Ala, Ala, Gly, Val, Ala, Gly, Val, Ile, Lys, Met, Val, Leu, Ala, Leu, Arg, His, Gly, Ilegctcctcgga, cgttgcatgt, ggacgagccg, acctcgcagg, tggattggtc, gacgggtgcg, , , , , 3000Ala, Pro, Arg, Thr, Leu, His, Val, Asp, Glu, Pro, Thr, Ser, Gln, Val, Asp, Trp, Ser, Thr, Gly, Alagtggagctgt, tgaccgagga, gcgggtgtgg, cctgaggtgg, gtcgtcctcg, ccgggctgga, , , , , 3060Val, Glu, Leu, Leu, Thr, Glu, Glu, Arg, Val, Trp, Pro, Glu, Val, Gly, Arg, Pro, Arg, Arg, Ala, Glygtgtccgcgt, tcggggtcag, tggcaccaac, gccccgtcat, ctggaacagg, cggccccgac, , , , , 3120Val, Ser, Ala, Phe, Gly, Val, Ser, Gly, Thr, Asn, Ala, Pro, Ser, Ser, Gly, Thr, Gly, Gly, Pro, Aspggcgaccgag, tcggccgatc, cggcgctgtc, ctggccgaag, ggcgtccctg, tgccgctggt, , , , , 3180Gly, Asp, Arg, Val, Gly, Arg, Ser, Gly, Ala, Val, Leu, Ala, Glu, Gly, Arg, Pro, Cys, Ala, Ala, Glyggtgtccggc, cgaggcgccg, cggcgctcgc, cgcccaggcg, caacggctac, ggaccttcgt, , , , , 3240Gly, Val, Arg, Pro, Arg, Arg, Arg, Gly, Ala, Arg, Arg, Pro, Gly, Ala, Thr, Ala, Thr, Asp, Leu, Argagccgacgag, ccgcaactcg, acttgagcga, actcggctac, gcgttgggtt, gtggtcgggc, , , , , 3300Ser, Arg, Arg, Ala, Ala, Thr, Arg, Leu, Glu, Arg, Thr, Arg, Leu, Arg, Val, Gly, Leu, Trp, Ser, GlyGgggttgtcg, gatcgtgggg, tggtggtggc, gggtggtcg, , , , , , , , , , , 3339Gly, Val, Val, Gly, Ser, Trp, Gly, Gly, Gly, Gly, Gly, Trp, the Ser genes and Riff-3:Rifamycin I type PKS module 1-3 homologys (52%);spnA:Saccharopolyspora spinosa (thorn saccharopolyspora strain) PKS is assembled and the homology of extending element 1 (53%), is related to the biosynthesis of spinosyn glucosides;Vene:Streptomyces venezuelae, I type PKS homologys (56%);AVES:The macrolide antibiotics avermectin I type PKS-AVES homologys (54%) 4 that Streptomyces avermitilis are produced;TylG:Tylactone synthase starting module and module 1,2 homology (56%) that Streptomyces fradiae are produced rif1-3 VDEEVAS----------------------------------------------------- 3608SpnA SAGPGSGSVVDVP----------------------------------------------- 1435AVES VDATGPADLTEPQEEAAEPECVADAVTEMSAEPECVADAMSEMSAECVAEAVSDKSAEPE 3151tylG PEAPDVTDVTEALEAPDATEAEGAKAPGSPEE---------------------------- 3051vene VAMAGTAGTSEVAEGSEASEAP--AAPGSREA---------------------------- 3079gdnD --SSGTG----------------------------------------------------- 1037 Amino acid sequence analysis finds the domain guarded containing typical I types PKS (see Fig. 8):Including acyltransferase (AT), acyl carrier protein (ACP), the N-terminal domain of beta-keto acyl base synthase (KS), the C-terminal domain of beta-keto acyl base synthase (KS), the N-terminal domain of thioesterase.
The present invention, which also clones from coemid pCGBA10 and analyzes other 5, participates in the new gene that geldanamycin exports mechanism from intracellular.22.gdn1:Gene size is 1479bp, encodes 493 amino acid.gtggccgctg ctgccgcgct gagcgcgtgc ggcacaccgg aagcacacgg aagacccacg 60Val Ala Ala Ala Ala Ala Leu Ser Ala Cys Gly Thr Pro Glu Ala His Gly Arg Pro Thrggggtggcga tggagccggc ggcaccggcg cagtacgtac tgatcactca gtgcttgcag 120Gly Val Ala Met Glu Pro Ala Ala Pro Ala Gln Tyr Val Leu Ile Thr Gln Cys Leu Glnaacgacttct tcctcaacct ggactgccag ttgtccctgc cggacagcgc cgtctccaag 180Asn Asp Phe Phe Leu Asn Leu Asp Cys Gln Leu Ser Leu Pro Asp Ser Ala Val Ser Lysctgctgctgg acagcgagag cggtgcgtcc ctccacacgg agggccaccg cagggtcctg 240Leu Leu Leu Asp Ser Glu Ser Gly Ala Ser Leu His Thr Glu Gly His Arg Arg Val Leutccgagtcgg agctgcgccg ttcgcccctg gcccgtttcc tcgacgccac cgtgggctcc 300Ser Glu Ser Glu Leu Arg Arg Ser Pro Leu Ala Arg Phe Leu Asp Ala Thr Val Gly Sercgtacgcgcg gacacgggga cggggttctg catctgatca acatccgtga ctggcatgtc 360Arg Thr Arg Gly His Gly Asp Gly Val Leu His Leu Ile Asn Ile Arg Asp Trp His Valccgggagaga catacgacct ggagcgcagg cagtacgggg cccattgcga ggccgacacc 420Pro Gly Glu Thr Tyr Asp Leu Glu Arg Arg Gln Tyr Gly Ala His Cys Glu Ala Asp Thrtggggggcgg cgtacgtcga cgggctcacg gacctgctgg ccccggatga gcgcgcgccc 480Trp Gly Ala Ala Tyr Val Asp Gly Leu Thr Asp Leu Leu Ala Pro Asp Glu Arg Ala Progcggacggcg agggcggctg gggcgggaaa ctccatgtcc accatgtgcg gtccaacacc 540Ala Asp Gly Glu Gly Gly Trp Gly Gly Lys Leu His Val His His Val Arg Ser Asn Thrctcttcgact tccagcacag cgccggcgga cgtcccgacc tcagcgaacc ggcgccgctg 600Leu Phe Asp Phe Gln His Ser Ala Gly Gly Arg Pro Asp Leu Ser Glu Pro Ala Pro Leuaccacactgc tggacggtct gctgggcgat ggacgtcagg agacgacgca tgtcgcggtg 660Thr Thr Leu Leu Asp Gly Leu Leu Gly Asp Gly Arg Gln Glu Thr Thr His Val Ala Valgtcggcgtcc tcaccgatat caaggtccag ctgctgctga ccggtatccg ctcccgctac 720Val Gly Val Leu Thr Asp Ile Lys Val Gln Leu Leu Leu Thr Gly Ile Arg Ser Arg Tyrgacgtacggc agctcgtcgt ctccgacgcg ctcaccgcca gcaggaccct ggagcgccat 780Asp Val Arg Gln Leu Val Val Ser Asp Ala Leu Thr Ala Ser Arg Thr Leu Glu Arg Hisctgacggcgc tggacttctg ccagcgtgtg ctgcgcaccg aggtgatgat cggcctggcg 840Leu Thr Ala Leu Asp Phe Cys Gln Arg Val Leu Arg Thr Glu Val Met Ile Gly Leu Alagagctggccc gtttcctggg ctcccggccg gacgacgacc ggctgtcccg tggcggggac 900Glu Leu Ala Arg Phe Leu Gly Ser Arg Pro Asp Asp Asp Arg Leu Ser Arg Gly Gly Aspgaggagttcg tgggctactc ctcatacatc caggacaagc agggcatcct gtcgtacgag 960Glu Glu Phe Val Gly Tyr Ser Ser Tyr Ile Gln Asp Lys Gln Gly Ile Leu Ser Tyr Glugacgccagga tgcgggacta tcgcatccag acctcggaac gcctccggcg gacgcagcac 1020Asp Ala Arg Met Arg Asp Tyr Arg Ile Gln Thr Ser Glu Arg Leu Arg Arg Thr Gln Hisacggtcggct tcgccagcaa gttcctgctg gggctgggca cctgtctgct ggtcagcgcc 1080Thr Val Gly Phe Ala Ser Lys Phe Leu Leu Gly Leu Gly Thr Cys Leu Leu Val Ser Alacttgtgctgt cgctcgtcgg cgtcttcctc ccgggccgta tcgggtggca gagccccgcc 1140Leu Val Leu Ser Leu Val Gly Val Phe Leu Pro Gly Arg Ile Gly Trp Gln Ser Pro Alagtgctcgggg cgctcgggg ggggcagatcg tcacgttgtt cttcacccg gccggtcaga 1200Val Leu Gly Ala Leu Gly Val Gly Gln Ile Val Thr Leu Phe Phe Thr Arg Pro Val Argtcggtgcagg acgcgctgg cggaggagac catctaccgga tgatcctgga gagccgcagc 1260Ser Val Gln Asp Ala Leu Ala Glu Glu Thr Ile Tyr Arg Met Ile Leu Glu Ser Arg Serctgaaggtgg ccctggcgc ggttccacat caccacggcc accgcgctccg acggcatgac 1320Leu Lys Val Ala Leu Ala Arg Phe His Ile Thr Thr Ala Thr Ala Leu Arg Arg His Aspgatgtgaacg gccaatccg acgccctggc acgccagttg gagatcctgga gaagatcgac 1380Asp Val Asn Gly Gln Ser Asp Ala Leu Ala Arg Gln Leu Glu Ile Leu Glu Lys Ile Aspacggccgact tcgaacggct gaaacagctg ggggtgaccc cgcgggccga atcgtccggg 1440Thr Ala Asp Phe Glu Arg Leu Lys Gln Leu Gly Val Thr Pro Arg Ala Glu Ser Ser Glyacggggcggc cccgcagaag aatccgctca caggtttcc 1479Thr Gly Arg Pro Arg Arg Arg Ile Arg Ser Gln Val Ser23.gdn2:Gene size is 1521bp, encodes 507 amino acid.atgtcaacac cacctatcgc cggtgacgac cagacgccgc gccggctrag gctgcgccgc 60Met Ser Thr Pro Pro Ile Ala Gly Asp Asp Gln Thr Pro Arg Arg XXX Arg Leu Arg Argcgcagggccg acgccgaccg cgggcgccgg ggcgggcggg catccgcacg gcggttcccc 120Arg Arg Ala Asp Ala Asp Arg Gly Arg Arg Gly Gly Arg Ala Ser Ala Arg Arg Phe Progatggcgccc tgccgcagcc cgagcccgtc gcgtcggatg tcatccgggc cggtgacagc 180Asp Gly Ala Leu Pro Gln Pro Glu Pro Val Ala Ser Asp Val Ile Arg Ala Gly Asp Seracctggctcc gggaccgggc gcgcaagcac ggcgcgtcgg cggccacccg gaaggtcttc 240Thr Trp Leu Arg Asp Arg Ala Arg Lys His Gly Ala Ser Ala Ala Thr Arg Lys Val Phegacccctggg tcctggccgg cccggaccgg gtgccctact tcgccgaact ggccagtctg 300Asp Pro Trp Val Leu Ala Gly Pro Asp Arg Val Pro Tyr Phe Ala Glu Leu Ala Ser Leucgcaaccggg tcaagcaccg gctcgccgag gagcatgcgc gagccgagga ggacggcgcg 360Arg Asn Arg Val Lys His Arg Leu Ala Glu Glu His Ala Arg Ala Glu Glu Asp Gly Alactggaggcga gccgggtcag ggcggccgcc accgcggccg gagagcggct ggagcgggcc 420Leu Glu Ala Ser Arg Val Arg Ala Ala Ala Thr Ala Ala Gly Glu Arg Leu Glu Arg Alagggcagcggc rggtcgtcct ggagcggcagc agacggtcac caccgcccag ctggaccgg 480Gly Gln Arg XXX Val Val Leu Glu Arg Gln Gln Thr Val Thr Thr Ala Gln Leu Asp Argctggcgcggc gggccgaccg gtggcagacc ttccgcgaca ccgtgcgggg cggtttcgag 540Leu Ala Arg Arg Ala Asp Arg Trp Gln Thr Phe Arg Asp Thr Val Arg Gly Gly Phe Glucgccggtggc tgcgcgcccg tatgcctgcc gacggcagcg acgggaccga ccccggacgg 600Arg Arg Trp Leu Arg Ala Arg Met Pro Ala Asp Gly Ser Asp Gly Thr Asp Pro Gly Argcagggcgcca cccggcgcga ggacgagccg gagaccaccg gccacgccag ctggcaggcg 660Gln Gly Ala Thr Arg Arg Glu Asp Glu Pro Glu Thr Thr Gly His Ala Ser Trp Gln Alagtgtccgaac ccgatccggt ggcggaggcg gacgcggccg accgcgccct gtccaccagg 720Val Ser Glu Pro Asp Pro Val Ala Glu Ala Asp Ala Ala Asp Arg Ala Leu Ser Thr Arggcggcgtggg agggcgcggc ggcgcgcccc gggatgccgc gctggatgaa gctcggtgtg 780Ala Ala Trp Glu Gly Ala Ala Ala Arg Pro Gly Met Pro Arg Trp Met Lys Leu Gly Valctggccgcgt tggtcgtggt ggaactgccc gtctactact cggtgttcga gaatctgcac 840Leu Ala Ala Leu Val Val Val Glu Leu Pro Val Tyr Tyr Ser Val Phe Glu Asn Leu Hisggtgtcgggc gcttcgccga tctgctctcc tacagcctca tggtggccgt ggcggtggcg 900Gly Val Gly Arg Phe Ala Asp Leu Leu Ser Tyr Ser Leu Met Val Ala Val Ala Val Alaatgatcctcg ccccgcatat cgcgggctgg atactgcggc ggcgctccgc caccggtgcg 960Met Ile Leu Ala Pro His Ile Ala Gly Trp Ile Leu Arg Arg Arg Ser Ala Thr Gly Alagtccggctgt cggccgtgcc cgccctcgcc ctgctgggcg tgtgggcgta cggcgcctgg 1020Val Arg Leu Ser Ala Val Pro Ala Leu Ala Leu Leu Gly Val Trp Ala Tyr Gly Ala Trpgcgctggggg atctgcgggc caaggtggcg ttccgggagg agcctccgct ggatctgccg 1080Ala Leu Gly Asp Leu Arg Ala Lys Val Ala Phe Arg Glu Glu Pro Pro Leu Asp Leu Procccgatgtgg ccgcggacgt gggcgacagc gtgcgcaacc cgccgagcct cctggagtcc 1140Pro Asp Val Ala Ala Asp Val Gly Asp Ser Val Arg Asn Pro Pro Ser Leu Leu Glu Serctgcatctgg acgcgcagag cgtgacctgg atgttcgtcg cgctgctgct gctctccggc 1200Leu His Leu Asp Ala Gln Ser Val Thr Trp Met Phe Val Ala Leu Leu Leu Leu Ser Glygggatcgcct tcctgatcgg gctgggcgag gagcatccgt atctcgcggc gtaccggacc 1260Gly Ile Ala Phe Leu Ile Gly Leu Gly Glu Glu His Pro Tyr Leu Ala Ala Tyr Arg Thracggccgagc ggctgcggga gctggagcgg gacatggaga cggatctcgc gggttccgag 1320Thr Ala Glu Arg Leu Arg Glu Leu Glu Arg Asp Met Glu Thr Asp Leu Ala Gly Ser Glucgtgccaagg aggccgaggc caccctgggt gcccgcgcgg aggcccgccg cgcggcccat 1380Arg Ala Lys Glu Ala Glu Ala Thr Leu Gly Ala Arg Ala Glu Ala Arg Arg Ala Ala Hisgaggcgcggc tctacgcggt cgacgatctc tacgaagccg cggcccacgc ctatctggac 1440Glu Ala Arg Leu Tyr Ala Val Asp Asp Leu Tyr Glu Ala Ala Ala His Ala Tyr Leu Aspggggtggcca tggagtccag cgatccggcg gtcacggagg ccgccatgcg gctgtccaag 1500Gly Val Ala Met Glu Ser Ser Asp Pro Ala Val Thr Glu Ala Ala Met Arg Leu Ser Lyscagtggccgc tgctgccgcg c 1521Gln Trp Pro Leu Leu Pro Arg24.gdn3:Gene size is 1791bp,Encode 597 amino acid .atgatcaaag, acgccaggcc, cccggaaccg, ttccagtatg, acccggcgtc, aggcatctac, , , , , 60Met, Ile, Lys, Asp, Ala, Arg, Pro, Pro, Glu, Pro, Phe, Gln, Tyr, Asp, Pro, Ala, Ser, Gly, Ile, Tyrgagggcgttc, tccggttgac, ttccgggcgt, tttcaggagc, gggccctatg, gggagcattc, , , , , 120Glu, Gly, Val, Leu, Arg, Leu, Thr, Ser, Gly, Arg, Phe, Gln, Glu, Arg, Ala, Leu, Trp, Gly, Ala, Pheccgggtacca, cctcaccgat, acggtctgac, agagaatcca, atcgacatcc, acatcggcat, , , , , 180Pro, Gly, Thr, Thr, Ser, Pro, Ile, Arg, Ser, Asp, Arg, Glu, Ser, Asn, Arg, His, Pro, His, Arg, Hiscgacaacggc, atccacatcg, gcgtcggcta, catcggccgc, gaaccctttg, cgggagaaag, , , , , 240Arg, Gln, Arg, His, Pro, His, Arg, Arg, Arg, Leu, His, Arg, Pro, Arg, Thr, Leu, Cys, Gly, Arg, Lyscggaatacca, ctgtccgcaa, tgcggctgtc, cggtgcggga, atttccgtac, cggggaaatc, , , , , 300Arg, Asn, Thr, Thr, Val, Arg, Asn, Ala, Ala, Val, Arg, Cys, Gly, Asn, Phe, Arg, Thr, Gly, Glu, Ilegggggatcca, tttccatgat, cacatctgac, agtgtcaacg, gggtggtgcg, ccgcggcagg, , , , , 360Gly, Gly, Ser, Ile, Ser, Met, Ile, Thr, Ser, Asp, Ser, Val, Asn, Gly, Val, Val, Arg, Arg, Gly, Argctcggccgta, ccgcccgctt, cgcggcccgc, tggcggggca, agcgcgacgg, cgcgcgcggc, , , , , 420Leu, Gly, Arg, Thr, Ala, Arg, Phe, Ala, Ala, Arg, Trp, Arg, Gly, Lys, Arg, Asp, Gly, Ala, Arg, Glygtcccccgca, tcgtgctccc, ggagccgtcc, ggggagcagc, ggagcaagac, gccgccgccc, , , , , 480Val, Pro, Arg, Ile, Val, Leu, Pro, Glu, Pro, Ser, Gly, Glu, Gln, Arg, Ser, Lys, Thr, Pro, Pro, Proatcgagccac, ccgctcccga, actgctgatc, accccttacg, tgatggaggt, gcggaccggc, , , , , 540Ile, Glu, Pro, Pro, Ala, Pro, Glu, Leu, Leu, Ile, Thr, Pro, Tyr, Val, Met, Glu, Val, Arg, Thr, Glygtccgccgaa, ccaccgagca, gatgcgctcc, gccctcatcg, ggcgggagca, cgccctgctc, , , , , 600Val, Arg, Arg, Thr, Thr, Glu, Gln, Met, Arg, Ser, Ala, Leu, Ile, Gly, Arg, Glu, His, Ala, Leu, Leuagcaggttgc, gcgccgagtc, ggtgcgcgtg, gtcacccagt, acgacgtccg, cgaggacccc, , , , , 660Ser, Arg, Leu, Arg, Ala, Glu, Ser, Val, Arg, Val, Val, Thr, Gln, Tyr, Asp, Val, Arg, Glu, Asp, Procggcccgcgg, sgctcgcgcg, ctacggccac, tgggtgggtc, agtggcgcac, cagcgtggac, , , , , 720Arg, Pro, Ala, XXX, Leu, Ala, Arg, Tyr, Gly, His, Trp, Val, Gly, Gln, Trp, Arg, Thr, Ser, Val, Aspcggtgccgat, cgcatgccca, tgccgtggtg, gaccaggcca, atcagcgact, nssnmtgmta, , , , , 780Arg, Cys, Arg, Ser, His, Ala, His, Ala, Val, Val, Asp, Gln, Ala, Asn, Gln, Arg, XXX, XXX, XXX, XXXmtgggacgcg, gtgmgcgaga, cccaccccca, gctctcccgc, ctcccccggc, gcccgcccgg, , , , , 840XXX, Gly, Arg, Gly, XXX, Arg, Asp, Pro, Pro, Pro, Ala, Leu, Pro, Pro, Pro, Pro, Ala, Pro, Ala, Argggactggctg, cccggccggg, tggagctgga, ccggtcctgg, taccagcccg, acgtctggct, , , , , 900Gly, Leu, Ala, Ala, Arg, Pro, Gly, Gly, Ala, Gly, Pro, Val, Leu, Val, Pro, Ala, Arg, Arg, Leu, Alagctggccgac, gacgacagca, cgcggacggc, cacctcccgg, gcgctgcaca, tactcgaacg, , , , , 960Ala, Gly, Arg, Arg, Arg, Gln, His, Ala, Asp, Gly, His, Leu, Pro, Gly, Ala, Ala, His, Thr, Arg, Thrgcagaacacc, gaccgcktcg, acrggaggac, cgcgtgatga, ccgtccacac, tcccgytccg, , , , , 1020Ala, Glu, His, Arg, Pro, XXX, Arg, XXX, Glu, Asp, Arg, Val, Met, Thr, Val, His, Thr, Pro, XXX, Progagcgccckg, ccgyccggsg, gcacggaaac, cggcggcacg, gaaaccggcg, gcgcgcgctg, , , , , 1080Glu, Arg, XXX, Ala, XXX, Arg, XXX, His, Gly, Asn, Arg, Arg, His, Gly, Asn, Arg, Arg, Arg, Ala, LeucccgccgSgc, tggtgctcgc, tctckcggcg, gccgccacgg, cctgcggctc, cgacgagccg, , , , , 1140Pro, Ala, XXX, Leu, Val, Leu, Ala, Leu, XXX, Ala, Ala, Ala, Thr, Ala, Cys, Gly, Ser, Asp, Glu, Protcacgctact, cgcagacatg, tggtgtcgtg, gtcgacggct, ccggctcggc, cgacgcctcc, , , , , 1200Ser, Arg, Tyr, Ser, Gln, Thr, Cys, Gly, Val, Val, Val, Asp, Gly, Ser, Gly, Ser, Ala, Asp, Ala, Sercggaccggct, tcgacgcgga, ggccaagctc, aaggccaccc, tccagacgtt, cctgtcggac, , , , , 1260Arg, Thr, Gly, Phe, Asp, Ala, Glu, Ala, Lys, Leu, Lys, Ala, Thr, Leu, Gln, Thr, Phe, Leu, Ser, Aspaagaagtgcc, gcaagacgtc, cttcgccccc, ataaccaagg, tttccgaggc, gtcgaagtgc, , , , , 1320Lys, Lys, Cys, Arg, Lys, Thr, Ser, Phe, Ala, Pro, Ile, Thr, Lys, Val, Ser, Glu, Ala, Ser, Lys, Cyscaggtcagcc, cgctcgacct, ggacccggac, acctcgaaga, ccgccgaccg, cgagcggacc, , , , , 1380Gln, Val, Ser, Pro, Leu, Asp, Leu, Asp, Pro, Asp, Thr, Ser, Lys, Thr, Ala, Asp, Arg, Glu, Arg, Thrcgcaccgcca, tgcgtgccgt, cgccctctcc, aacgccctga, agctgctgcg, ctgcgcccag, , , , , 1440Arg, Thr, Ala, Met, Arg, Ala, Val, Ala, Leu, Ser, Asn, Ala, Leu, Lys, Leu, Leu, Arg, Cys, Ala, Glnaaggaggagc, ccggctccga, tgtgctcggc, gggctgtcgc, gcatcgcgct, gtccaagccg, , , , , 1500Lys, Glu, Glu, Pro, Gly, Ser, Asp, Val, Leu, Gly, Gly, Leu, Ser, Arg, Ile, Ala, Leu, Ser, Lys, Proagcggtgacg, acgcgtcgtt, cgacgtcctg, gtggtcagcg, acttcgacca, gggcgacacc, , , , , 1560Ser, Gly, Asp, Asp, Ala, Ser, Phe, Asp, Val, Leu, Val, Val, Ser, Asp, Phe, Asp, Gln, Gly, Asp, Thrgacttccggc, tcgggcggca, ggacctgtcc, accgccacca, gccgccggac, cgtcatcgac, , , , , 1620Asp, Phe, Arg, Leu, Gly, Arg, Gln, Asp, Leu, Ser, Thr, Ala, Thr, Ser, Arg, Arg, Thr, Val, Ile, Aspgacttcctca, agtcgcacgg, caaaccgaag, ctgtccggcg, ccgatgtcta, cccggtgggc, , , , , 1680Asp, Phe, Leu, Lys, Ser, His, Gly, Lys, Pro, Lys, Leu, Ser, Gly, Ala, Asp, Val, Tyr, Pro, Val, Glytacggcatga, agtaccacac, cgacacctyc, cggtacgagc, agttcaacgc, cttctggacg, , , , , 1740Tyr, Gly, Met, Lys, Tyr, His, Thr, Asp, Thr, XXX, Arg, Tyr, Glu, Gln, Phe, Asn, Ala, Phe, Trp, Thrgagcttctgg, aggggagggt, caaggcacat, gtcaacacca, cctatcgccg, g, , , , , , , 1791Glu, Leu, Leu, Glu, Gly, Arg, Val, Lys, Ala, His, Val, Asn, Thr, Thr, Tyr, Arg, Arg25.gdn4:Gene size is 705bp, encodes 235 amino acid.gtggagaacg tcccagagcg cgcagagccc acgctccgga tcagtcagac acacttcccg 60Val Glu Asn Val Pro Glu Arg Ala Glu Pro Thr Leu Arg Ile Ser Gln Thr His Phe Progtggagaccc tgggtcccgg gcggcgcctg gccgtctggt cgcaggggtg cggactggcc 120Val Glu Thr Leu Gly Pro Gly Arg Arg Leu Ala Val Trp Ser Gln Gly Cys Gly Leu Alatgcgccggct gtatgtcccg gcacacctgg gatccgcgag gcggcgcctc tcgtacggtg 180Cys Ala Gly Cys Met Ser Arg His Thr Trp Asp Pro Arg Gly Gly Ala Ser Arg Thr Valtcgtccctgc tcgggctgtg gcgcgaggcg ttggcgcgcg gcgcggacgg gctgacgatc 240Ser Ser Leu Leu Gly Leu Trp Arg Glu Ala Leu Ala Arg Gly Ala Asp Gly Leu Thr Ileagcggcgggg agccgctcga ccagcccgcc gctctggagg ccctgctggc cggggcggtg 300Ser Gly Gly Glu Pro Leu Asp Gln Pro Ala Ala Leu Glu Ala Leu Leu Ala Gly Ala Valcgggcccgtg cggaggcggt ggcatcgggc ggcccggcgg cgggccgtga gatcgacatc 360Arg Ala Arg Ala Glu Ala Val Ala Ser Gly Gly Pro Ala Ala Gly Arg Glu Ile Asp Ilectcctctaca cggggtacga ggaggacgaa gtggagcgtg acgcggcgcg ctccgccgcc 420Leu Leu Tyr Thr Gly Tyr Glu Glu Asp Glu Val Glu Arg Asp Ala Ala Arg Ser Ala Alagtccgccacg ccgatgcgct ggtgaccgga cgcttccggg tggccgagcc caccgcgctg 480Val Arg His Ala Asp Ala Leu Val Thr Gly Arg Phe Arg Val Ala Glu Pro Thr Ala Leugtgtggcgcg gctcggcgaa ccagcgcata cggccgcgta cggcgcgcgg gtgggcgcgc 540Val Trp Arg Gly Ser Ala Asn Gln Arg Ile Arg Pro Arg Thr Ala Arg Gly Trp Ala Argtaccaggagc atctgrmmcg racggagagc gggccgcgtc tacaggtggw cgagggggag 600Tyr Gln Glu His Leu XXX XXX Thr Glu Ser Gly Pro Arg Leu Gln Val XXX Glu Gly Gluggcgatgtgc ggctctacgg agtgccgcgg cgcggcgaac tggtcgagct ggagcgtcgg 660Gly Asp Val Arg Leu Tyr Gly Val Pro Arg Arg Gly Glu Leu Val Glu Leu Glu Arg Argttgcggcggg cggggatcgc cctcaccggt gcgagctggc gcccc 705Leu Arg Arg Ala Gly Ile Ala Leu Thr Gly Ala Ser Trp Arg Pro26.gdn5:Gene size is 1218bp, encodes 406 amino acid.Atgtgcggct, ctacggagtg, ccgcggcgcg, gcgaactggt, cgagctggag, cgtcggttgc, , , , , 60Met, Cys, Gly, Ser, Thr, Glu, Cys, Arg, Gly, Ala, Ala, Asn, Trp, Ser, Ser, Trp, Ser, Val, Gly, Cysggcgggcggg, gatcgccctc, accggtgcga, gctggcgccc, ctgagcgggg, gcgcccgnct, , , , , 120Gly, Gly, Arg, Gly, Ser, Pro, Ser, Pro, Val, Arg, Ala, Gly, Ala, Pro, Glu, Arg, Gly, Arg, Pro, XXXcgggtggcag, ggcgcgccgc, gaccccggaa, gccgtggcct, cggcggctgt, ggtgcttgcg, , , , , 180Arg, Val, Ala, Gly, Arg, Ala, Ala, Thr, Pro, Glu, Ala, Val, Ala, Ser, Ala, Ala, Val, Val, Leu, Alagcagcggacc, ttgtggcggt, ggccttggcg, gctgtggtcc, ctatggctgg, gtgctcggcg, , , , , 240Ala, Ala, Asp, Leu, Val, Ala, Val, Ala, Leu, Ala, Ala, Val, Val, Pro, Met, Ala, Gly, Cys, Ser, Alagcgatggcct, cggcggytgt, ggtccttgcg, gcasgggccg, ttgtggcaga, gcgccctgtg, , , , , 300Ala, Met, Ala, Ser, Ala, XXX, Val, Val, Leu, Ala, Ala, XXX, Ala, Val, Val, Ala, Glu, Arg, Pro, Valgcggtggtct, cggcggctgc, gatcctctac, gccggacgca, tcgtggccgg, catcaccggc, , , , , 360Ala, Val, Val, Ser, Ala, Ala, Ala, Ile, Leu, Tyr, Ala, Gly, Arg, Ile, Val, Ala, Gly, Ile, Thr, Glygccacaggtg, cggttgctgg, cgcctatatc, gccgacatca, ccgatgggga, agatcgggct, , , , , 420Ala, Thr, Gly, Ala, Val, Ala, Gly, Ala, Tyr, Ile, Ala, Asp, Ile, Thr, Asp, Gly, Glu, Asp, Arg, Alacgccacttcg, ggctcatgag, cgcttgtttc, ggcgtgggta, tggtggcagg, ccccgtggcc, , , , , 480Arg, His, Phe, Gly, Leu, Met, Ser, Ala, Cys, Phe, Gly, Val, Gly, Met, Val, Ala, Gly, Pro, Val, Alagggggactgt, tgggcgccat, ctccttgcat, gcaccattcc, ttgcggcggc, ggtgctcaac, , , , , 540Gly, Gly, Leu, Leu, Gly, Ala, Ile, Ser, Leu, His, Ala, Pro, Phe, Leu, Ala, Ala, Ala, Val, Leu, Asnggcctcaacc, tactactggg, ctgcttccta, atgcaggagt, cgcataaggg, agagcgtcga, , , , , 600Gly, Leu, Asn, Leu, Leu, Leu, Gly, Cys, Phe, Leu, Met, Gln, Glu, Ser, His, Lys, Gly, Glu, Arg, Argccgatgccct, tgagagcctt, caacccagtc, agctccttcc, ggtgggcgcg, gggcatgact, , , , , 660Pro, Met, Pro, Leu, Arg, Ala, Phe, Asn, Pro, Val, Ser, Ser, Phe, Arg, Trp, Ala, Arg, Gly, Met, Thratcgtcgccg, cacttatgac, tgtcttcttt, atcatgcaac, tcgtaggaca, ggtgccggca, , , , , 720Ile, Val, Ala, Ala, Leu, Met, Thr, Val, Phe, Phe, Ile, Met, Gln, Leu, Val, Gly, Gln, Val, Pro, Alagcgctctggg, tcattttcgg, cgaggaccgc, tttcgctgga, gcgcgacgat, gatcggcctg, , , , , 780Ala, Leu, Trp, Val, Ile, Phe, Gly, Glu, Asp, Arg, Phe, Arg, Trp, Ser, Ala, Thr, Met, Ile, Gly, Leutcgcttgcgg, tattcggaat, cttgcacgcc, ctcgctcaag, ccttcgtcac, tggtcccgcc, , , , , 840Ser, Leu, Ala, Val, Phe, Gly, Ile, Leu, His, Ala, Leu, Ala, Gln, Ala, Phe, Val, Thr, Gly, Pro, Alaaccaaacgtt, tcggcgagaa, gcaggccatt, atcgccggca, tggcggccga, cgcgctgggc, , , , , 900Thr, Lys, Arg, Phe, Gly, Glu, Lys, Gln, Ala, Ile, Ile, Ala, Gly, Met, Ala, Ala, Asp, Ala, Leu, GlyTacgtcttgc, tggcgttcgc, gacgcgaggc, tggatggcct, tccccattat, gattcttctc, , , , , 960Tyr, Val, Leu, Leu, Ala, Phe, Ala, Thr, Arg, Gly, Trp, Met, Ala, Phe, Pro, Ile, Met, Ile, Leu, Leugcttccggcg, gcatcgggat, gcccgcgttg, caggccatgc, tgtccaggca, ggtagatgac, , , , , 1020Ala, Ser, Gly, Gly, Ile, Gly, Met, Pro, Ala, Leu, Gln, Ala, Met, Leu, Ser, Arg, Gln, Val, Asp, Aspgaccatcagg, gacagcttca, aggatcgctc, gcggctctta, ccagcctaac, ttcgatcatt, , , , , 1080Asp, His, Gln, Gly, Gln, Leu, Gln, Gly, Ser, Leu, Ala, Ala, Leu, Thr, Ser, Leu, Thr, Ser, Ile, Ileggaccgctga, tcgtcacggc, gatttatgcc, gcctcggcga, gcacatggaa, cgggttggca, , , , , 1140Gly, Pro, Leu, Ile, Val, Thr, Ala, Ile, Tyr, Ala, Ala, Ser, Ala, Ser, Thr, Trp, Asn, Gly, Leu, Alatggattgtag, gcgccgccct, ataccttgtc, tgcctccccg, cgttgcgtcg, cggtgcatgg, , , , , 1200Trp, Ile, Val, Gly, Ala, Ala, Leu, Tyr, Leu, Val, Cys, Leu, Pro, Ala, Leu, Arg, Arg, Gly, Ala, Trpagccgggcca, cctcgacc, , , , , , , , , , , , , , , , , 1218Ser, Arg, Ala, Thr, Ser, the Thr genes and tetracycline resistance gene, (, tetrac, ), very high homology, (, 93%, ),,Similitude (93%),Known tetracycline resistance gene encodes efflux protein,Be responsible for antibiotic is discharged it is extracellular.Gdn1-5 gene linkages, therefore can consider that this group of gene is extracellular related to the output of geldanamycin.Invention effect:
Present invention clone obtains the biosynthesis gene of geldanamycin, available for the genetic manipulation for being intended to improve the antibiotic yield or structure of modification.
Brief description of the drawings
Fig. 1 designs primer PCR product electrophoresis with AHBA conserved sequences
Wherein:1 molecular weight marker 200bp ladder
2 PCR primer 755bp
3 molecular weight marker λ DNA/HindIII
Fig. 2 4.0kb containing BamHI fragment gene cluster enzyme spectrum analysis
Wherein:pCGBA1、pCGBA10、pCGBA12、pCGBA9、pCGBA13、
PCGBA15, pCGBA17 number for positive colony coemid
Fig. 3 gdnB conservative domains
Wherein:Pp-binding is the attachment site of phosphopantetheine
Fig. 4 gdnF conservative domains
Wherein:Acyltransf2 is N- acyltransferase domains
Fig. 5 gdnH conservative domains
Wherein contain P450 domains
Fig. 6 geldanamycin biosynthesis genes encoder block arranges organization chart
Fig. 7 gdnC conservative domains
Fig. 8 gdnD conservative domains
Ketoacyl-synt:The N-terminal domain of beta-keto acyl base synthase (KS);Ketoacyl-synt_c:
The C-terminal domain of beta-keto acyl base synthase (KS);
:The N-terminal domain of thioesterase
Sequence table<110>Inst. of Medicinal Biological Technology, Chinese Academy of Medical Sciences<120>The gene cluster of water-absorption rules biosynthesis geldanamycin<160>52<170>PatentIn version 3.1<210>1<211>1233<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1)..(1233)<223><400>1gtg agc cag ttc ata gaa gat gtc cgg aac tca tcg atg ccc gca tcc 48Val Ser Gln Phe Ile Glu Asp Val Arg Asn Ser Ser Met Pro Ala Ser1 5 10 15gac gac tca acc ggc gcg tgt ccg cct gcc gcc gtt gcc ccg agc cag 96Asp Asp Ser Thr Gly Ala Cys Pro Pro Ala Ala Val Ala Pro Ser Gln
20 25 30gag tcg tcc atg tcc gct ggc ccc ctc gcc ccg gcc acc cca tgg cgc 144Glu Ser Ser Met Ser Ala Gly Pro Leu Ala Pro Ala Thr Pro Trp Arg
35 40 45agg gcg acg ggt gcc gct gta cgt cct cgt ccc gct ggc cgc ggc cat 192Arg Ala Thr Gly Ala Ala Val Arg Pro Arg Pro Ala Gly Arg Gly His
50 55 60cgc cgt gat cgg cta cta cct gcc cct gct cgg cat ccg cct cgc cgc 240Arg Arg Asp Arg Leu Leu Pro Ala Pro Ala Arg His Pro Pro Arg Arg65 70 75 80ctt cct ggc cgt cga cat cgc cgc ggg cga gat cgc ccg cgg ccg cgc 288Leu Pro Gly Arg Arg His Arg Arg Gly Arg Asp Arg Pro Arg Pro Arg
85 90 95cac cat ccc ggc cgc ctg acc ccg gtt tgc cat ctc ggc aat atg ttt 336His His Pro Gly Arg Leu Thr Pro Val Cys His Leu Gly Asn Met Phe
100 105 110tgc ctc gat gga ggg tgg gct cgc atg gtt gcc gtg gag gtg gtc aag 384Cys Leu Asp Gly Gly Trp Ala Arg Met Val Ala Val Glu Val Val Lys
115 120 125gtg gcc gat gag ctg aag aac gcc ttt gac gtg gtg gtg atc ggc ggt 432Val Ala Asp Glu Leu Lys Asn Ala Phe Asp Val Val Val Ile Gly Gly
130 135 140ggc gcc gct ggg ctg agc ggg gcg ctg atg ctg gcc cgg tcg cgg cgt 480Gly Ala Ala Gly Leu Ser Gly Ala Leu Met Leu Ala Arg Ser Arg Arg145 150 155 160tcg gtg gtg gtg atc gac gcg ggc gcc ccg cgc aac gcc ccg gcc tcg 528Ser Val Val Val Ile Asp Ala Gly Ala Pro Arg Asn Ala Pro Ala Ser
165 170 175gcg gtg cac gga ctg ctg gcc cgg gac ggg atc cct ccg gcc gag ttg 576Ala Val His Gly Leu Leu Ala Arg Asp Gly Ile Pro Pro Ala Glu Leu
180 185 190gtg gcc cgg ggc cgg gcc gag gtc cgc ggc tac ggc ggt cag gtg gtg 624Val Ala Arg Gly Arg Ala Glu Val Arg Gly Tyr Gly Gly Gln Val Val
195 200 205tcc ggc gag gtg ggc gcc gtg acc cgg gag gag tcc ggg ggc ttc cag 672Ser Gly Glu Val Gly Ala Val Thr Arg Glu Glu Ser Gly Gly Phe Gln
210 215 220gtg gcc ctg acc gat ggc cgg acc gta cgt gcg cgc cgg ttg ctg ctg 720Val Ala Leu Thr Asp Gly Arg Thr Val Arg Ala Arg Arg Leu Leu Leu225 230 235 240gcc acc ggg ctg gtc gac gag ttg ccg gac atc ccg ggg ctg cgg tcc 768Ala Thr Gly Leu Val Asp Glu Leu Pro Asp Ile Pro Gly Leu Arg Ser
245 250 255cgg tgg ggc cgg gat gtg ctg cac tgt ccg tac tgc cac ggc tgg gag 816Arg Trp Gly Arg Asp Val Leu His Cys Pro Tyr Cys His Gly Trp Glu
260 265 270gtc cgc gac cag gcc atc ggc gta ctg ggg agc ggg ccg ctg tcg gtg 864Val Arg Asp Gln Ala Ile Gly Val Leu Gly Ser Gly Pro Leu Ser Val
275 280 285cac cag gcg ctg ctg ttc cgt cag tgg agc gac gat gtc acc ttc ttc 912His Gln Ala Leu Leu Phe Arg Gln Trp Ser Asp Asp Val Thr Phe Phe
290 295 300ccc cac acc ctg ccg tcg ccg tcc ggc gag gag gcg gag cag ctg gcc 960Pro His Thr Leu Pro Ser Pro Ser Gly Glu Glu Ala Glu Gln Leu Ala305 310 315 320gcc cgt ggc atc cgt gtg gtg gac ggc gag gtg gcg tcc ttg gag atc 1008Ala Arg Gly Ile Arg Val Val Asp Gly Glu Val Ala Ser Leu Glu Ile
325 330 335gtc gag gac cgc ctc gtc ggc gtg cgg ctg ggc gac ggc ggc gtg gtc 1056Val Glu Asp Arg Leu Val Gly Val Arg Leu Gly Asp Gly Gly Val Val
340 345 350gag cgc gag gcg ctg gcc gtc gcg ccg cgg atg gtg gca cac gcc ggt 1104Glu Arg Glu Ala Leu Ala Val Ala Pro Arg Met Val Ala His Ala Gly
355 360 365ctc ctg gcg ggg ctc ggg ctg cgg ccg gtg gag cat ccg agc ggc ggc 1152Leu Leu Ala Gly Leu Gly Leu Arg Pro Val Glu His Pro Ser Gly Gly
370 375 380ggt gag cac atc ccg tcc gac gcg acc ggg cgc acc gag gtg tcc ggg 1200Gly Glu His Ile Pro Ser Asp Ala Thr Gly Arg Thr Glu Val Ser Gly385 390 395 400gtg tgg gtc gcg ggc aat gtc acc gat ctg gcg 1233Val Trp Val Ala Gly Asn Val Thr Asp Leu Ala
405 410<210>2<211>411<212>PRT<213>Streptomyces hygroscopicus<400>2Val Ser Gln Phe Ile Glu Asp Val Arg Asn Ser Ser Met Pro Ala Ser1 5 10 15Asp Asp Ser Thr Gly Ala Cys Pro Pro Ala Ala Val Ala Pro Ser Gln
20 25 30Glu Ser Ser Met Ser Ala Gly Pro Leu Ala Pro Ala Thr Pro Trp Arg
35 40 45Arg Ala Thr Gly Ala Ala Val Arg Pro Arg Pro Ala Gly Arg Gly His
50 55 60Arg Arg Asp Arg Leu Leu Pro Ala Pro Ala Arg His Pro Pro Arg Arg65 70 75 80Leu Pro Gly Arg Arg His Arg Arg Gly Arg Asp Arg Pro Arg Pro Arg
85 90 95His His Pro Gly Arg Leu Thr Pro Val Cys His Leu Gly Asn Met Phe
100 105 110Cys Leu Asp Gly Gly Trp Ala Arg Met Val Ala Val Glu Val Val Lys
115 120 125Val Ala Asp Glu Leu Lys Asn Ala Phe Asp Val Val Val Ile Gly Gly
130 135 140Gly Ala Ala Gly Leu Ser Gly Ala Leu Met Leu Ala Arg Ser Arg Arg145 150 155 160Ser Val Val Val Ile Asp Ala Gly Ala Pro Arg Asn Ala Pro Ala Ser
165 170 175Ala Val His Gly Leu Leu Ala Arg Asp Gly Ile Pro Pro Ala Glu Leu
180 185 190Val Ala Arg Gly Arg Ala Glu Val Arg Gly Tyr Gly Gly Gln Val Val
195 200 205Ser Gly Glu Val Gly Ala Val Thr Arg Glu Glu Ser Gly Gly Phe Gln
210 215 220Val Ala Leu Thr Asp Gly Arg Thr Val Arg Ala Arg Arg Leu Leu Leu225 230 235 240Ala Thr Gly Leu Val Asp Glu Leu Pro Asp Ile Pro Gly Leu Arg Ser
245 250 255Arg Trp Gly Arg Asp Val Leu His Cys Pro Tyr Cys His Gly Trp Glu
260 265 270Val Arg Asp Gln Ala Ile Gly Val Leu Gly Ser Gly Pro Leu Ser Val
275 280 285His Gln Ala Leu Leu Phe Arg Gln Trp Ser Asp Asp Val Thr Phe Phe
290 295 300Pro His Thr Leu Pro Ser Pro Ser Gly Glu Glu Ala Glu Gln Leu Ala305 310 315 320Ala Arg Gly Ile Arg Val Val Asp Gly Glu Val Ala Ser Leu Glu Ile
325 330 335Val Glu Asp Arg Leu Val Gly Val Arg Leu Gly Asp Gly Gly Val Val
340 345 350Glu Arg Glu Ala Leu Ala Val Ala Pro Arg Met Val Ala His Ala Gly
355 360 365Leu Leu Ala Gly Leu Gly Leu Arg Pro Val Glu His Pro Ser Gly Gly
370 375 380Gly Glu His Ile Pro Ser Asp Ala Thr Gly Arg Thr Glu Val Ser Gly385 390 395 400Val Trp Val Ala Gly Asn Val Thr Asp Leu Ala
405 410<210>3<211>819<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1)..(819)<223><400>3atg ggg tca tgt ggc ggc ggt cca cga gcc gcg ctt gtc atc ggc gtg 48Met Gly Ser Cys Gly Gly Gly Pro Arg Ala Ala Leu Val Ile Gly Val1 5 10 15atg aag att gag cea tcg tta cgc ata ccg gca acg get ggc ace atg 96Met Lys Ile Glu Pro Ser Leu Arg Ile Pro Ala Thr Ala Gly Thr Met
20 25 30gct gcc atg tcc gcc ggg ccg ccc ttc cgt ctc gca cga gcc gcc gtg 144Ala Ala Met Ser Ala Gly Pro Pro Phe Arg Leu Ala Arg Ala Ala Val
35 40 45ttc gcg gcg atg tgc gtc gtg gtg acg gcg ctc gga cac gtg ctg atg 192Phe Ala Ala Met Cys Val Val Val Thr Ala Leu Gly His Val Leu Met
50 55 60tcc ggt gac agg ctg ccg gtg tgg gcc gtg gcc gcc gcc ttc gcc gga 240Ser Gly Asp Arg Leu Pro Val Trp Ala Val Ala Ala Ala Phe Ala Gly65 70 75 80acg gcg gcc ggt gcg tgg tgg gtt gcg ggg cgg gag cac ggt gcg ctg 288Thr Ala Ala Gly Ala Trp Trp Val Ala Gly Arg Glu His Gly Ala Leu
85 90 95gcc gtg acc ggg gcg acc gtg gtc gcg caa ttc ggc ctc cat atg gcc 336Ala Val Thr Gly Ala Thr Val Val Ala Gln Phe Gly Leu His Met Ala
100 105 110ttc cgg ttc gcg gag acg gca gtc gcc cca gcg gcg gga agc gcc atg 384Phe Arg Phe Ala Glu Thr Ala Val Ala Pro Ala Ala Gly Ser Ala Met
115 120 125ggt gac ggg atg tcc ggt atg cgg ggc ggc atg ggc gcc gcc ccg atg 432Gly Asp Gly Met Ser Gly Met Arg Gly Gly Met Gly Ala Ala Pro Met
130 135 140agc ggc gcc gcc atg ggt cat atg cac gat ggc atg ggc cat atg cgc 480Ser Gly Ala Ala Met Gly His Met His Asp Gly Met Gly His Met Arg145 150 155 160cat ggc gcg gac gcg atc tcc tcc gcc gcg ccg tcc atg agt cat ctg 528His Gly Ala Asp Ala Ile Ser Ser Ala Ala Pro Ser Met Ser His Leu
165 170 175ccg tgg ccc tgg gcg ggt ccg ggc ggg gcg ggc atg gcc acg gcc cac 576Pro Trp Pro Trp Ala Gly Pro Gly Gly Ala Gly Met Ala Thr Ala His
180 185 190ctg ctc gcc gcc ctg atc tgc ggg ctg tgg ctg tgg cgc ggc gaa cgg 624Leu Leu Ala Ala Leu Ile Cys Gly Leu Trp Leu Trp Arg Gly Glu Arg
195 200 205gcc gcc ttc cgg ctc ggc cgc gcg ctc gcg gcc ctg ctg ttc gtc ccg 672Ala Ala Phe Arg Leu Gly Arg Ala Leu Ala Ala Leu Leu Phe Val Pro
210 215 220ctc gtc ctc gcc ctg cgc atc ctg ggc gcg ggt gtc act ccg ccg ccc 720Leu Val Leu Ala Leu Arg Ile Leu Gly Ala Gly Val Thr Pro Pro Pro225 230 235 240gca tgg acc tcc gca ccg gcc gtc gcc cgc cgg ccg cgc gga gtc ctg 768Ala Trp Thr Ser Ala Pro Ala Val Ala Arg Arg Pro Arg Gly Val Leu
245 250 255ctg cgg cac gtc atc ctg cgc aga ggg cca ccg agg cgg ttc gcc atc 816Leu Arg His Val Ile Leu Arg Arg Gly Pro Pro Arg Arg Phe Ala Ile
260 265 270cgc 819Arg<210>4<211>273<212>PRT<213>Streptomyces hygroscopicus<400>4Met Gly Ser Cys Gly Gly Gly Pro Arg Ala Ala Leu Val Ile Gly Val1 5 10 15Met Lys Ile Glu Pro Ser Leu Arg Ile Pro Ala Thr Ala Gly Thr Met
20 25 30Ala Ala Met Ser Ala Gly Pro Pro Phe Arg Leu Ala Arg Ala Ala Val
35 40 45Phe Ala Ala Met Cys Val Val Val Thr Ala Leu Gly His Val Leu Met
50 55 60Ser Gly Asp Arg Leu Pro Val Trp Ala Val Ala Ala Ala Phe Ala Gly65 70 75 80Thr Ala Ala Gly Ala Trp Trp Val Ala Gly Arg Glu His Gly Ala Leu
85 90 95Ala Val Thr Gly Ala Thr Val Val Ala Gln Phe Gly Leu His Met Ala
100 105 110Phe Arg Phe Ala Glu Thr Ala Val Ala Pro Ala Ala Gly Ser Ala Met
115 120 125Gly Asp Gly Met Ser Gly Met Arg Gly Gly Met Gly Ala Ala Pro Met
130 135 140Ser Gly Ala Ala Met Gly His Met His Asp Gly Met Gly His Met Arg145 150 155 160His Gly Ala Asp Ala Ile Ser Ser Ala Ala Pro Ser Met Ser His Leu
165 170 175Pro Trp Pro Trp Ala Gly Pro Gly Gly Ala Gly Met Ala Thr Ala His
180 185 190Leu Leu Ala Ala Leu Ile Cys Gly Leu Trp Leu Trp Arg Gly Glu Arg
195 200 205Ala Ala Phe Arg Leu Gly Arg Ala Leu Ala Ala Leu Leu Phe Val Pro
210 215 220Leu Val Leu Ala Leu Arg Ile Leu Gly Ala Gly Val Thr Pro Pro Pro225 230 235 240Ala Trp Thr Ser Ala Pro Ala Val Ala Arg Arg Pro Arg Gly Val Leu
245 250 255Leu Arg His Val Ile Leu Arg Arg Gly Pro Pro Arg Arg Phe Ala Ile
260 265 270Arg<210>5<211>630<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1)..(630)<223><400>5atg cct tct gcc acg ctg ccc gcc gca ccg ata aga gcc gtg cac ggc 48Met Pro Ser Ala Thr Leu Pro Ala Ala Pro Ile Arg Ala Val His Gly1 5 10 15ctt gcc acc gcg gcg aac gac cac cag gtc acc gaa tgg gcg ctg gcc 96Leu Ala Thr Ala Ala Asn Asp His Gln Val Thr Glu Trp Ala Leu Ala
20 25 30gcc cgg gac ggc gac cgc gag gcg gtc gac cac ttc atc cgc gcc acc 144Ala Arg Asp Gly Asp Arg Glu Ala Val Asp His Phe Ile Arg Ala Thr
35 40 45tac cgc gat gtg cgc cgt ttc gtg ctc cac ctc agc gcc gat ccg cat 192Tyr Arg Asp Val Arg Arg Phe Val Leu His Leu Ser Ala Asp Pro His
50 55 60ggt tgt gag gac crc gcc cag gag acg tat ctg cgg gcg ctg acc ggg 240Gly Cys Glu Asp Leu Ala Gln Glu Thr Tyr Leu Arg Ala Leu Thr Gly65 70 75 80ctg ccg cgc ttc gcc ggt cgc tca tcg gcc cgg acg tgg ctg ctg tcg 288Leu Pro Arg Phe Ala Gly Arg Ser Ser Ala Arg Thr Trp Leu Leu Ser
85 90 95atc gcc cgc cgt gtg gtc gtc gac cgc tac cgc acg gcc gcc gcc cgt 336Ile Ala Arg Arg Val Val Val Asp Arg Tyr Arg Thr Ala Ala Ala Arg
100 105 110ccc cgt acg ttg gac gcg gac gac tgg cag gag gcg gcc gaa cgg gcg 384Pro Arg Thr Leu Asp Ala Asp Asp Trp Gln Glu Ala Ala Glu Arg Ala
115 120 125cag ccc gcc ggg ctc ccc ggg ttc gac gag ggg gtg gcg ctg atg gac 432Gln Pro Ala Gly Leu Pro Gly Phe Asp Glu Gly Val Ala Leu Met Asp
130 135 140ctg ctg gcg gcg ctc gcc ccg gca cgc cgt gag atg ttc ctc ctc acc 480Leu Leu Ala Ala Leu Ala Pro Ala Arg Arg Glu Met Phe Leu Leu Thr145 150 155 160aag gtg ctc ggc ctg ccg tac gcg gac gcc gcg acc gcg acc ggc tgc 528Lys Val Leu Gly Leu Pro Tyr Ala Asp Ala Ala Thr Ala Thr Gly Cys
165 170 175ccc atc ggc acc gta cgc tcg cgc gtg gcc cgc gcc cgt gag gac atc 576Pro Ile Gly Thr Val Arg Ser Arg Val Ala Arg Ala Arg Glu Asp Ile
180 185 190tcc gcg ctg ctg gcc gcg gcc gag aag gcc gcg gga ccg gtg ccg ttg 624Ser Ala Leu Leu Ala Ala Ala Glu Lys Ala Ala Gly Pro Val Pro Leu
195 200 205gtg ggc 630Val Gly
210<210>6<211>210<212>PRT<213>Streptomyces hygroscopicus<400>6Met Pro Ser Ala Thr Leu Pro Ala Ala Pro Ile Arg Ala Val His Gly1 5 10 15Leu Ala Thr Ala Ala Asn Asp His Gln Val Thr Glu Trp Ala Leu Ala
20 25 30Ala Arg Asp Gly Asp Arg Glu Ala Val Asp His Phe Ile Arg Ala Thr
35 40 45Tyr Arg Asp Val Arg Arg Phe Val Leu His Leu Ser Ala Asp Pro His
50 55 60Gly Cys Glu Asp Leu Ala Gln Glu Thr Tyr Leu Arg Ala Leu Thr Gly65 70 75 80Leu Pro Arg Phe Ala Gly Arg Ser Ser Ala Arg Thr Trp Leu Leu Ser
85 90 95Ile Ala Arg Arg Val Val Val Asp Arg Tyr Arg Thr Ala Ala Ala Arg
100 105 110Pro Arg Thr Leu Asp Ala Asp Asp Trp Gln Glu Ala Ala Glu Arg Ala
115 120 125Gln Pro Ala Gly Leu Pro Gly Phe Asp Glu Gly Val Ala Leu Met Asp
130 135 140Leu Leu Ala Ala Leu Ala Pro Ala Arg Arg Glu Met Phe Leu Leu Thr145 150 155 160Lys Val Leu Gly Leu Pro Tyr Ala Asp Ala Ala Thr Ala Thr Gly Cys
165 170 175Pro Ile Gly Thr Val Arg Ser Arg Val Ala Arg Ala Arg Glu Asp Ile
180 185 190Ser Ala Leu Leu Ala Ala Ala Glu Lys Ala Ala Gly Pro Val Pro Leu
195 200 205Val Gly
210<210>7<211>993<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1)..(993)<223><400>7atg agg tcc gcc gga cca tcg gcg cca tcg aac ggg tct acg cct cgg 48Met Arg Ser Ala Gly Pro Ser Ala Pro Ser Asn Gly Ser Thr Pro Arg1 5 10 15ccc gga tcc acc ggg agg tcc ggg agt cgg cgt cgg tgt gac cgc acc 96Pro Gly Ser Thr Gly Arg Ser Gly Ser Arg Arg Arg Cys Asp Arg Thr
20 25 30ggc cga ccg ccc cgc cgc acc ggc tcc gct ccc atc cgc ccc ctc cct 144Gly Arg Pro Pro Arg Arg Thr Gly Ser Ala Pro Ile Arg Pro Leu Pro
35 40 45tcc ctc gcc atc cct ttc gtc gtc ttc ccc ttc tcc gga cca cgt tct 192Ser Leu Ala Ile Pro Phe Val Val Phe Pro Phe Ser Gly Pro Arg Ser
50 55 60ttc aga ccg cgt tct ttc gga ccg cat tct tcg gac cgc gtt ctt cgg 240Phe Arg Pro Arg Ser Phe Gly Pro His Ser Ser Asp Arg Val Leu Arg65 70 75 80acc gca ccc gag acc cgg acc ggc ggt ccg ccg tac cgg ccc gca cca 288Thr Ala Pro Glu Thr Arg Thr Gly Gly Pro Pro Tyr Arg Pro Ala Pro
85 90 95cgg gag tgc tca atg aac acc cat ccg atc agt cat ggc ggc ccg ctc 336Arg Glu Cys Ser Met Asn Thr His Pro Ile Ser His Gly Gly Pro Leu
100 105 110tcc ggc gcg ggt gtc gcc ccc atc acc tcg gtg gtc ttc gac ctc gac 384Ser Gly Ala Gly Val Ala Pro Ile Thr Ser Val Val Phe Asp Leu Asp
115 120 125ggt gtc ctc gtc aac agc ttc gcg gtg atg cgc gag gcg ttc acc ctc 432Gly Val Leu Val Asn Ser Phe Ala Val Met Arg Glu Ala Phe Thr Leu
130 135 140gcc tac gcc gag gtc gtc ggc gac ggt gag cca ccc ttc gag gag tac 480Ala Tyr Ala Glu Val Val Gly Asp Gly Glu Pro Pro Phe Glu Glu Tyr145 150 155 160aac cgg cat ctg ggc cgc tac ttc ccc gac atc atg cgg atc atg ggt 528Asn Arg His Leu Gly Arg Tyr Phe Pro Asp Ile Met Arg Ile Met Gly
165 170 175ctt ccg ctg gag atg gag ggc ccg ttc gtc cgc gag agc tac cgg ctc 576Leu Pro Leu Glu Met Glu Gly Pro Phe Val Arg Glu Ser Tyr Arg Leu
180 185 190gcc cac ctg gtg gag atg ttc gac ggt gtg cca gag ctg ctg tcg gag 624Ala His Leu Val Glu Met Phe Asp Gly Val Pro Glu Leu Leu Ser Glu
195 200 205ctg cgc cac cgc ggg tta cga ctc gcc gtg gcc acc ggg aag agc gga 672Leu Arg His Arg Gly Leu Arg Leu Ala Val Ala Thr Gly Lys Ser Gly
210 215 220ccc cgg gcg cgt tcg ctg ctc gac acc ctc ggc atc cgt ggg cag ttc 720Pro Arg Ala Arg Ser Leu Leu Asp Thr Leu Gly Ile Arg Gly Gln Phe225 230 235 240cac gtg gtc ctc ggc tcg gac gag gtg gcc cgg ccc aag ccc gcg ccg 768His Val Val Leu Gly Ser Asp Glu Val Ala Arg Pro Lys Pro Ala Pro
245 250 255gac atc gtg ctg aag gcg atg gac atg atg gac gcg gac ccc gac cgg 816Asp Ile Val Leu Lys Ala Met Asp Met Met Asp Ala Asp Pro Asp Arg
260 265 270acc gtg atg gtc ggg gac gcg gtg acc gac ctg gcc agc gcg cgg ggg 864Thr Val Met Val Gly Asp Ala Val Thr Asp Leu Ala Ser Ala Arg Gly
275 280 285gcc ggg atc acc gcc gtg gcc gcg atg tgg ggt gag acc gac gag aag 912Ala Gly Ile Thr Ala Val Ala Ala Met Trp Gly Glu Thr Asp Glu Lys
290 295 300acc ctg ctc gcg gcg gag ccc gat gtg atc ctg cac aag ccg gcg gaa 960Thr Leu Leu Ala Ala Glu Pro Asp Val Ile Leu His Lys Pro Ala Glu305 310 315 320ctg ctg tcg ctg tgc ccc gag gtg acg gtt cca 993Leu Leu Ser Leu Cys Pro Glu Val Thr Val Pro
325 330<210>8<211>331<212>PRT<213>Streptomyces hygroscopicus<400>8Met Arg Ser Ala Gly Pro Ser Ala Pro Ser Asn Gly Ser Thr Pro Arg1 5 10 15Pro Gly Ser Thr Gly Arg Ser Gly Ser Arg Arg Arg Cys Asp Arg Thr
20 25 30Gly Arg Pro Pro Arg Arg Thr Gly Ser Ala Pro Ile Arg Pro Leu Pro
35 40 45Ser Leu Ala Ile Pro Phe Val Val Phe Pro Phe Ser Gly Pro Arg Ser
50 55 60Phe Arg Pro Arg Ser Phe Gly Pro His Ser Ser Asp Arg Val Leu Arg65 70 75 80Thr Ala Pro Glu Thr Arg Thr Gly Gly Pro Pro Tyr Arg Pro Ala Pro
85 90 95Arg Glu Cys Ser Met Asn Thr His Pro Ile Ser His Gly Gly Pro Leu
100 105 110Ser Gly Ala Gly Val Ala Pro Ile Thr Ser Val Val Phe Asp Leu Asp
115 120 125Gly Val Leu Val Asn Ser Phe Ala Val Met Arg Glu Ala Phe Thr Leu
130 135 140Ala Tyr Ala Glu Val Val Gly Asp Gly Glu Pro Pro Phe Glu Glu Tyr145 150 155 160Asn Arg His Leu Gly Arg Tyr Phe Pro Asp Ile Met Arg Ile Met Gly
165 170 175Leu Pro Leu Glu Met Glu Gly Pro Phe Val Arg Glu Ser Tyr Arg Leu
180 185 190Ala His Leu Val Glu Met Phe Asp Gly Val Pro Glu Leu Leu Ser Glu
195 200 205Leu Arg His Arg Gly Leu Arg Leu Ala Val Ala Thr Gly Lys Ser Gly
210 215 220Pro Arg Ala Arg Ser Leu Leu Asp Thr Leu Gly Ile Arg Gly Gln Phe225 230 235 240His Val Val Leu Gly Ser Asp Glu Val Ala Arg Pro Lys Pro Ala Pro
245 250 255Asp Ile Val Leu Lys Ala Met Asp Met Met Asp Ala Asp Pro Asp Arg
260 265 270Thr Val Met Val Gly Asp Ala Val Thr Asp Leu Ala Ser Ala Arg Gly
275 280 285Ala Gly Ile Thr Ala Val Ala Ala Met Trp Gly Glu Thr Asp Glu Lys
290 295 300Thr Leu Leu Ala Ala Glu Pro Asp Val Ile Leu His Lys Pro Ala Glu305 310 315 320Leu Leu Ser Leu Cys Pro Glu Val Thr Val Pro
325 330<210>9<211>1131<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1)..(1131)<223><400>9atg agc gcc ccg tcc atc ggc gag ccg ccg atc agg acc gcc gtg gtg 48Met Ser Ala Pro Ser Ile Gly Glu Pro Pro Ile Arg Thr Ala Val Val1 5 10 15ggg ctg gga tgg gcg gcc cgc tcg atc tgg ctg ccc cgg ctc cgc cac 96Gly Leu Gly Trp Ala Ala Arg Ser Ile Trp Leu Pro Arg Leu Arg His
20 25 30aac ccc gcc ttc acc gtg acc gcc gcg gtg gat ccc gac gag cgc ggc 144Asn Pro Ala Phe Thr Val Thr Ala Ala Val Asp Pro Asp Glu Arg Gly
35 40 45cgc gcg gcc gtc gcc gag gcg gag ggc atg gac cgg ctg ccg gtg ctg 192Arg Ala Ala Val Ala Glu Ala Glu Gly Met Asp Arg Leu Pro Val Leu
50 55 60gcg gcc gtc cac gac ctc gac ccc gcc gag gtg gac ctg gcg gtg gtc 240Ala Ala Val His Asp Leu Asp Pro Ala Glu Val Asp Leu Ala Val Val65 70 75 80gcg gtg ccc aac cat ctg cac tgt gcg gtc gcc gcc gag ctg ctg gcc 288Ala Val Pro Asn His Leu His Cys Ala Val Ala Ala Glu Leu Leu Ala
85 90 95aag ggc att ccg gtg ttc ctg gag aag ccg gtg tgc ctg acc tcc gag 336Lys Gly Ile Pro Val Phe Leu Glu Lys Pro Val Cys Leu Thr Ser Glu
100 105 110gag gcc gag cgg ctg gcc gaa gcg gag cgc tcg ggc ggc gcg atg ctg 384Glu Ala Glu Arg Leu Ala Glu Ala Glu Arg Ser Gly Gly Ala Met Leu
115 120 125ctg gcc ggc agc gcg gcg cgg tac cgc gcc gat gtg cgc ggg ctg tac 432Leu Ala Gly Ser Ala Ala Arg Tyr Arg Ala Asp Val Arg Gly Leu Tyr
130 135 140cgg atc gcc gcc cgg ctg ggc cat atc cgt cat gtc gag ctc gcc tgg 480Arg Ile Ala Ala Arg Leu Gly His Ile Arg His Val Glu Leu Ala Trp145 150 155 160gtg cgg tca cgc ggc gtg ccc gac cgg ggc ggc tgg ttc acc cag cgg 528Val Arg Ser Arg Gly Val Pro Asp Arg Gly Gly Trp Phe Thr Gln Arg
165 170 175tcg ctc gcg ggc ggc ggg gcg ctg gtc gac ctg ggc tgg cat ctg ttc 576Ser Leu Ala Gly Gly Gly Ala Leu Val Asp Leu Gly Trp His Leu Phe
180 185 190gac atc gcg gtt ccg ctg ctg ggc acc gcc gcg ttc cgg cac gcc atc 624Asp Ile Ala Val Pro Leu Leu Gly Thr Ala Ala Phe Arg His Ala Ile
195 200 205ggg acc gtg tcc tcc gac ttc atc gtc cag cgg tcc tcc cgg gcc gcg 672Gly Thr Val Ser Ser Asp Phe Ile Val Gln Arg Ser Ser Arg Ala Ala
210 215 220tgg cgc ggc gac gac ggc gac ggc ccg gcg ctc ctg ggc gcc aac ggg 720Trp Arg Gly Asp Asp Gly Asp Gly Pro Ala Leu Leu Gly Ala Asn Gly225 230 235 240ggt gcc acc gat gtc gag gac acc gca cgc gga ttc ctc atc acc gac 768Gly Ala Thr Asp Val Glu Asp Thr Ala Arg Gly Phe Leu Ile Thr Asp
245 250 255gac ggc cgt tcg gtc gtg ctg cac gcg agc tgg gcc tcg cac gag gaa 816Asp Gly Arg Ser Val Val Leu His Ala Ser Trp Ala Ser His Glu Glu
260 265 270ctg gac acc acc cgg gtg acg atc gac ggc agc gcg ggc agc gcc acc 864Leu Asp Thr Thr Arg Val Thr Ile Asp Gly Ser Ala Gly Ser Ala Thr
275 280 285ctg cgc tgc acc ttc gga ttc agc ccg aac cgc ctc gag aag tcc acc 912Leu Arg Cys Thr Phe Gly Phe Ser Pro Asn Arg Leu Glu Lys Ser Thr
290 295 300ctg acc cgt acc gtc gac ggt acg acc cgg ccg gtg gcc gta ccc acc 960Leu Thr Arg Thr Val Asp Gly Thr Thr Arg Pro Val Ala Val Pro Thr305 310 315 320gaa ccg gtc ggc acc gag tac gac cgg cag ctc gac ctg ctt ccc gcg 1008Glu Pro Val Gly Thr Glu Tyr Asp Arg Gln Leu Asp Leu Leu Pro Ala
325 330 335caa ctg cgc gac ccg gcc ggg cgg ggc cgg gtg atc gat gag gtc cgc 1056Gln Leu Arg Asp Pro Ala Gly Arg Gly Arg Val Ile Asp Glu Val Arg
340 345 350cgg acc atc ggc gcc atc gaa cgg gtc tac gcc tcg gcc cgg atc cac 1104Arg Thr Ile Gly Ala Ile Glu Arg Val Tyr Ala Ser Ala Arg Ile His
355 360 365cgg gag gtc cgg gag tcg gcg tcg gtg 1131Arg Glu Val Arg Glu Ser Ala Ser Val
370 375<210>10<211>377<212>PRT<213>Streptomyces hygroscopicus<400>10Met Ser Ala Pro Ser Ile Gly Glu Pro Pro Ile Arg Thr Ala Val Val1 5 10 15Gly Leu Gly Trp Ala Ala Arg Ser Ile Trp Leu Pro Arg Leu Arg His
20 25 30Asn Pro Ala Phe Thr Val Thr Ala Ala Val Asp Pro Asp Glu Arg Gly
35 40 45Arg Ala Ala Val Ala Glu Ala Glu Gly Met Asp Arg Leu Pro Val Leu
50 55 60Ala Ala Val His Asp Leu Asp Pro Ala Glu Val Asp Leu Ala Val Val65 70 75 80Ala Val Pro Asn His Leu His Cys Ala Val Ala Ala Glu Leu Leu Ala
85 90 95Lys Gly Ile Pro Val Phe Leu Glu Lys Pro Val Cys Leu Thr Ser Glu
100 105 110Glu Ala Glu Arg Leu Ala Glu Ala Glu Arg Ser Gly Gly Ala Met Leu
115 120 125Leu Ala Gly Ser Ala Ala Arg Tyr Arg Ala Asp Val Arg Gly Leu Tyr
130 135 140Arg Ile Ala Ala Arg Leu Gly His Ile Arg His Val Glu Leu Ala Trp145 150 155 160Val Arg Ser Arg Gly Val Pro Asp Arg Gly Gly Trp Phe Thr Gln Arg
165 170 175Ser Leu Ala Gly Gly Gly Ala Leu Val Asp Leu Gly Trp His Leu Phe
180 185 190Asp Ile Ala Val Pro Leu Leu Gly Thr Ala Ala Phe Arg His Ala Ile
195 200 205Gly Thr Val Ser Ser Asp Phe Ile Val Gln Arg Ser Ser Arg Ala Ala
210 215 220Trp Arg Gly Asp Asp Gly Asp Gly Pro Ala Leu Leu Gly Ala Asn Gly225 230 235 240Gly Ala Thr Asp Val Glu Asp Thr Ala Arg Gly Phe Leu Ile Thr Asp
245 250 255Asp Gly Arg Ser Val Val Leu His Ala Ser Trp Ala Ser His Glu Glu
260 265 270Leu Asp Thr Thr Arg Val Thr Ile Asp Gly Ser Ala Gly Ser Ala Thr
275 280 285Leu Arg Cys Thr Phe Gly Phe Ser Pro Asn Arg Leu Glu Lys Ser Thr
290 295 300Leu Thr Arg Thr Val Asp Gly Thr Thr Arg Pro Val Ala Val Pro Thr305 310 315 320Glu Pro Val Gly Thr Glu Tyr Asp Arg Gln Leu Asp Leu Leu Pro Ala
325 330 335Gln Leu Arg Asp Pro Ala Gly Arg Gly Arg Val Ile Asp Glu Val Arg
340 345 350Arg Thr Ile Gly Ala Ile Glu Arg Val Tyr Ala Ser Ala Arg Ile His
355 360 365Arg Glu Val Arg Glu Ser Ala Ser Val
370 375<210>11<211>1152<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1)..(1152)<223><400>11gtg cga ctg cga tcc gag ctg ccc gca tgg ccg cag tac ggc gac gag 48Val Arg Leu Arg Ser Glu Leu Pro Ala Trp Pro Gln Tyr Gly Asp Glu 1 5 10 15gag cgc gag gcc ctc atc cgg gct ctg gat cag ggg caa tgg tgg cgt 96Glu Arg Glu Ala Leu Ile Arg Ala Leu Asp Gln Gly Gln Trp Trp Arg
20 25 30atc ggg ggc ggt gag gtc gac gcc ttc gag gcg gag ttc gccgcg gcc 144Ile Gly Gly Gly Glu Val Asp Ala Phe Glu Ala Glu Phe Ala Ala Ala
35 40 45cat gga agc gag cac gcc ctg gcg gtc acc aac ggg acg cat gcg ctg 192His Gly Ser Glu His Ala Leu Ala Val Thr Asn Gly Thr His Ala Leu
50 55 60gag ctc gcc ctc gaa gtg ctc ggg gtc ggc gcc gac tcc gag gtg atc 240Glu Leu Ala Leu Glu Val Leu Gly Val Gly Ala Asp Ser Glu Val Ile65 70 75 80gtt ccc gcg ttc acc ttc atc tcg tcc tcg cag gcg gct cag cgg ctg 288Val Pro Ala Phe Thr Phe Ile Ser Ser Ser Gln Ala Ala Gln Arg Leu
85 90 95ggc gcg gtg gcc gtg ccc gtg gac gtg gac ccg gac acg tac tgc atc 336Gly Ala Val Ala Val Pro Val Asp Val Asp Pro Asp Thr Tyr Cys Ile
100 105 110gat ccc tca gcg gtc gag gcg gcc atc ggc ccg aaa acc cgc gcg atc 384Asp Pro Ser Ala Val Glu Ala Ala Ile Gly Pro Lys Thr Arg Ala Ile
115 120 125atg ccg gtg cac atg gcg ggc cag atg tgc gac atg gac gcg ctg ggc 432Met Pro Val His Met Ala Gly Gln Met Cys Asp Met Asp Ala Leu Gly
130 135 140aag ctg tcc gcc gac tcg ggg gtg ccg ctg atc cag gac gcg gcc cat 480Lys Leu Ser Ala Asp Ser Gly Val Pro Leu Ile Gln Asp Ala Ala His145 150 155 160gcg cac ggt gcg cgg tgg cgc ggt cag aag gtc ggt gag ctg ggc tcg 528Ala His Gly Ala Arg Trp Arg Gly Gln Lys Val Gly Glu Leu Gly Ser
165 170 175gtc gcc gcg ttc agc ttc cag aac gga aag ctg atg acg gcc ggc gag 576Val Ala Ala Phe Ser Phe Gln Asn Gly Lys Leu Met Thr Ala Gly Glu
180 185 190ggc ggc gcc gtg ctc ttc ccc gat gcc gag atg tac gag agg ggc ttc 624Gly Gly Ala Val Leu Phe Pro Asp Ala Glu Met Tyr Glu Arg Gly Phe
195 200 205gtc cgg cac agc tgt gga cgt ccg cgc acc gac cgc ggc tac ttc cac 672Val Arg His Ser Cys Gly Arg Pro Arg Thr Asp Arg Gly Tyr Phe His
210 215 220cgc acc tcg ggc tcc aac ttc cgg ctg aac gag ttc tcc gca tcg gta 720Arg Thr Ser Gly Ser Asn Phe Arg Leu Asn Glu Phe Ser Ala Ser Val225 230 235 240ctg cgc gcc caa ctc acc cgc ctg gac ggc cag atc acc acg cgt gag 768Leu Arg Ala Gln Leu Thr Arg Leu Asp Gly Gln Ile Thr Thr Arg Glu
245 250 255cag cgc tgg ccg gtg ctg agc agg ctg ctc gcc gag atc ccc ggt gtg 816Gln Arg Trp Pro Val Leu Ser Arg Leu Leu Ala Glu Ile Pro Gly Val
260 265 270gta ccg cag tcg cgc gac gac cgc ggt gac cgc aat ccg cac tac atg 864Val Pro Gln Ser Arg Asp Asp Arg Gly Asp Arg Asn Pro His Tyr Met
275 280 285gcg atg ttc cgg gtg ccg ggc atc acc gag gag cgc cgt gcg aag gtc 912Ala Met Phe Arg Val Pro Gly Ile Thr Glu Glu Arg Arg Ala Lys Val
290 295 300gtc gac acc ctc atc gag cgc ggg gtg ccc gcg ttc gtc gcg ttc cgc 960Val Asp Thr Leu Ile Glu Arg Gly Val Pro Ala Phe Val Ala Phe Arg305 310 315 320gcg gtc tac cgt acg gac gcc ttc tgg gag gtc gcg gcg ccg gat ctg 1008Ala Val Tyr Arg Thr Asp Ala Phe Trp Glu Val Ala Ala Pro Asp Leu
325 330 335acg gtg gac gaa ctc gcc cgc cgc tgc ccg cac tcc gag gcg ctc acc 1056Thr Val Asp Glu Leu Ala Arg Arg Cys Pro His Ser Glu Ala Leu Thr
340 345 350cgc gac tgc ctt tgg ctg cac cac cgg gtg ttg ctg ggc agc gag gag 1104Arg Asp Cys Leu Trp Leu His His Arg Val Leu Leu Gly Ser Glu Glu
355 360 365cag atg cac gaa gtg gcc gcc gtc gtc gcc gat gtg ctc gcg ggc gca 1152Gln Met His Glu Val Ala Ala Val Val Ala Asp Val Leu Ala Gly Ala
370 375 380<210>12<211>384<212>PRT<213>Streptomyces hygroscopicus<400>12Val Arg Leu Arg Ser Glu Leu Pro Ala Trp Pro Gln Tyr Gly Asp Glu1 5 10 15Glu Arg Glu Ala Leu Ile Arg Ala Leu Asp Gln Gly Gln Trp Trp Arg
20 25 30Ile Gly Gly Gly Glu Val Asp Ala Phe Glu Ala Glu Phe Ala Ala Ala
35 40 45His Gly Ser Glu His Ala Leu Ala Val Thr Asn Gly Thr His Ala Leu
50 55 60Glu Leu Ala Leu Glu Val Leu Gly Val Gly Ala Asp Ser Glu Val Ile65 70 75 80Val Pro Ala Phe Thr Phe Ile Ser Ser Ser Gln Ala Ala Gln Arg Leu
85 90 95Gly Ala Val Ala Val Pro Val Asp Val Asp Pro Asp Thr Tyr Cys Ile
100 105 110Asp Pro Ser Ala Val Glu Ala Ala Ile Gly Pro Lys Thr Arg Ala Ile
115 120 125Met Pro Val His Met Ala Gly Gln Met Cys Asp Met Asp Ala Leu Gly
130 135 140Lys Leu Ser Ala Asp Ser Gly Val Pro Leu Ile Gln Asp Ala Ala His145 150 155 160Ala His Gly Ala Arg Trp Arg Gly Gln Lys Val Gly Glu Leu Gly Ser
165 170 175Val Ala Ala Phe Ser Phe Gln Asn Gly Lys Leu Met Thr Ala Gly Glu
180 185 190Gly Gly Ala Val Leu Phe Pro Asp Ala Glu Met Tyr Glu Arg Gly Phe
195 200 205Val Arg His Ser Cys Gly Arg Pro Arg Thr Asp Arg Gly Tyr Phe His
210 215 220Arg Thr Ser Gly Ser Asn Phe Arg Leu Asn Glu Phe Ser Ala Ser Val225 230 235 240Leu Arg Ala Gln Leu Thr Arg Leu Asp Gly Gln Ile Thr Thr Arg Glu
245 250 255Gln Arg Trp Pro Val Leu Ser Arg Leu Leu Ala Glu Ile Pro Gly Val
260 265 270Val Pro Gln Ser Arg Asp Asp Arg Gly Asp Arg Asn Pro His Tyr Met
275 280 285Ala Met Phe Arg Val Pro Gly Ile Thr Glu Glu Arg Arg Ala Lys Val
290 295 300Val Asp Thr Leu Ile Glu Arg Gly Val Pro Ala Phe Val Ala Phe Arg305 310 315 320Ala Val Tyr Arg Thr Asp Ala Phe Trp Glu Val Ala Ala Pro Asp Leu
325 330 335Thr Val Asp Glu Leu Ala Arg Arg Cys Pro His Ser Glu Ala Leu Thr
340 345 350Arg Asp Cys Leu Trp Leu His His Arg Val Leu Leu Gly Ser Glu Glu
355 360 365Gln Met His Glu Val Ala Ala Val Val Ala Asp Val Leu Ala Gly Ala
370 375 380<210>13<211>444<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1)..(444)<223><400>13gtg agg tgc cca ttg agc agg ctg ttg ttg gtg aac gga ccg aat ctc 48Val Arg Cys Pro Leu Ser Arg Leu Leu Leu Val Asn Gly Pro Asn Leu1 5 10 15ggc ata ctc ggc aag cgc cag ccc gag atc tac ggc acg gat acg ctt 96Gly Ile Leu Gly Lys Arg Gln Pro Glu Ile Tyr Gly Thr Asp Thr Leu
20 25 30cag gac atc gag cgc tgg gtc ggg gaa gag gtc gcg gag cgc ggc tgg 144Gln Asp Ile Glu Arg Trp Val Gly Glu Glu Val Ala Glu Arg Gly Trp
35 40 45aaa gtg gat tcc tac cag ttc gat ggc gaa gcg gag atc atc cag acc 192Lys Val Asp Ser Tyr Gln Phe Asp Gly Glu Ala Glu Ile Ile Gln Thr
50 55 60att cag ggg aac tac gac acg gtc ggt gcc atc atc aat ccg gcc gcg 240Ile Gln Gly Asn Tyr Asp Thr Val Gly Ala Ile Ile Asn Pro Ala Ala65 70 75 80ctc atg atg gcc gga tgg ggc ctt cgg gac gca ctg gcg aac tat ccg 288Leu Met Met Ala Gly Trp Gly Leu Arg Asp Ala Leu Ala Asn Tyr Pro
85 90 95cgg ccc tgg ata gaa gtg cat ctg tcg aat gtc tgg gcc cgt gag cag 336Arg Pro Trp Ile Glu Val His Leu Ser Asn Val Trp Ala Arg Glu Gln
100 105 110ttc cgc cat gag tcg gtg acc gga ccg ctg gcc gcg ggt gtc atc ttc 384Phe Arg His Glu Ser Val Thr Gly Pro Leu Ala Ala Gly Val Ile Phe
115 120 125ggg ctc ggc gcc ctg ggc tac cgg ctc gcc gcc cgc gcc ctg ctc gac 432Gly Leu Gly Ala Leu Gly Tyr Arg Leu Ala Ala Arg Ala Leu Leu Asp
130 135 140aag gtg ccg gac 444Lys Val Pro Asp145<210>14<211>148<212>PRT<213>Streptomyces hygroscopicus<400>14Val Arg Cys Pro Leu Ser Arg Leu Leu Leu Val Asn Gly Pro Asn Leu1 5 10 15Gly Ile Leu Gly Lys Arg Gln Pro Glu Ile Tyr Gly Thr Asp Thr Leu
20 25 30Gln Asp Ile Glu Arg Trp Val Gly Glu Glu Val Ala Glu Arg Gly Trp
35 40 45Lys Val Asp Ser Tyr Gln Phe Asp Gly Glu Ala Glu Ile Ile Gln Thr
50 55 60Ile Gln Gly Asn Tyr Asp Thr Val Gly Ala Ile Ile Asn Pro Ala Ala65 70 75 80Leu Met Met Ala Gly Trp Gly Leu Arg Asp Ala Leu Ala Asn Tyr Pro
85 90 95Arg Pro Trp Ile Glu Val His Leu Ser Asn Val Trp Ala Arg Glu Gln
100 105 110Phe Arg His Glu Ser Val Thr Gly Pro Leu Ala Ala Gly Val Ile Phe
115 120 125Gly Leu Gly Ala Leu Gly Tyr Arg Leu Ala Ala Arg Ala Leu Leu Asp
130 135 140Lys Val Pro Asp145<210>15<211>882<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1)..(882)<223><400>15gtg gcg ctg cgc ctc gaa cac gac gac ctc ggc atc agc gaa tcc tcc 48Val Ala Leu Arg Leu Glu His Asp Asp Leu Gly Ile Ser Glu Ser Ser1 5 10 15ttc cgc tgg ccc gag ccg gac ggt acg gac gcc atg acg tcc gcg tcc 96Phe Arg Trp Pro Glu Pro Asp Gly Thr Asp Ala Met Thr Ser Ala Ser
20 25 30ggt ggc gcc acc cgc gat ctg gac ctg ctg gcg cgc cac atc agg gag 144Gly Gly Ala Thr Arg Asp Leu Asp Leu Leu Ala Arg His Ile Arg Glu
35 40 45ctg tgt gcg ggc cga ccc gag cgg ctc aca ggt gtc ggg gtc gcg atg 192Leu Cys Ala Gly Arg Pro Glu Arg Leu Thr Gly Val Gly Val Ala Met
50 55 60ccc gcc acc ctc gac gcc acc ggc acg gtc acc gcc tgg ccc ggc cgt 240Pro Ala Thr Leu Asp Ala Thr Gly Thr Val Thr Ala Trp Pro Gly Arg65 70 75 80ccc agc tgg gcc gga gtg gat ctg cgc ggc gcg ctg tcc gcc ctc ttc 288Pro Ser Trp Ala Gly Val Asp Leu Arg Gly Ala Leu Ser Ala Leu Phe
85 90 95ggc cac gcc gag gtg cgc tgc gcc gac gac ggc gat ctg gcc gcc ctc 336Gly His Ala Glu Val Arg Cys Ala Asp Asp Gly Asp Leu Ala Ala Leu
100 105 110gcc gaa gca cac gaa gcc cgc tgc ccc gac ctg ctc tat ctc ggc gtc 384Ala Glu Ala His Glu Ala Arg Cys Pro Asp Leu Leu Tyr Leu Gly Val
115 120 125ggc acc ggg ata ggc ggt ggc atc gtg ctg aac ggg aaa ccc gtg ccc 432Gly Thr Gly Ile Gly Gly Gly Ile Val Leu Asn Gly Lys Pro Val Pro
130 135 140ggt gtg ggc cgc ggc tcc tgc gaa gtc ggc cac ctg gtc gtg gac cgc 480Gly Val Gly Arg Gly Ser Cys Glu Val Gly His Leu Val Val Asp Arg145 150 155 160gac gga ccg ctg tgc gac tgc ggt cgg cgc ggc tgc gtc cag gcg gcg 528Asp Gly Pro Leu Cys Asp Cys Gly Arg Arg Gly Cys Val Gln Ala Ala
165 170 175gcc tcg ggc ccg gcg acc ctg cgc agg gcg gcg cgg aga cgg gac gag 576Ala Ser Gly Pro Ala Thr Leu Arg Arg Ala Ala Arg Arg Arg Asp Glu
180 185 190gag gtg acc ttc acc gcg ctg cgc caa gcg gtg cgc ggc gga aag ccg 624Glu Val Thr Phe Thr Ala Leu Arg Gln Ala Val Arg Gly Gly Lys Pro
195 200 205tgg gcg gtg gcg tcg ctg cgg gag agc ggc agg gcc ctg gcc gcg gcc 672Trp Ala Val Ala Ser Leu Arg Glu Ser Gly Arg Ala Leu Ala Ala Ala
210 215 220gtg acc ggc gta tgc gaa ctg ctc cat ccc tcg ctc gtg ctg atc ggc 720Val Thr Gly Val Cys Glu Leu Leu His Pro Ser Leu Val Leu Ile Gly225 230 235 240gga ggg ttt gcc gcg gcg atg ccg gag ctg gtg gcg atg gtg gcc gag 768Gly Gly Phe Ala Ala Ala Met Pro Glu Leu Val Ala Met Val Ala Glu
245 250 255cgg acg gcg gag ctg ggg cgc ccc ggc cat cca ccg cca ccg gtc cgg 816Arg Thr Ala Glu Leu Gly Arg Pro Gly His Pro Pro Pro Pro Val Arg
260 265 270ccc gcg cga ctg ggc ggg ctg tcc tca ctg cac ggc gcc gtg ctg ctg 864Pro Ala Arg Leu Gly Gly Leu Ser Ser Leu His Gly Ala Val Leu Leu
275 280 285gcc agg gga ctg ccg gac 882Ala Arg Gly Leu Pro Asp
290<210>16<211>294<212>PRT<213>Streptomyces hygroscopicus<400>16Val Ala Leu Arg Leu Glu His Asp Asp Leu Gly Ile Ser Glu Ser Ser1 5 10 15Phe Arg Trp Pro Glu Pro Asp Gly Thr Asp Ala Met Thr Ser Ala Ser
20 25 30Gly Gly Ala Thr Arg Asp Leu Asp Leu Leu Ala Arg His Ile Arg Glu
35 40 45Leu Cys Ala Gly Arg Pro Glu Arg Leu Thr Gly Val Gly Val Ala Met
50 55 60Pro Ala Thr Leu Asp Ala Thr Gly Thr Val Thr Ala Trp Pro Gly Arg65 70 75 80Pro Ser Trp Ala Gly Val Asp Leu Arg Gly Ala Leu Ser Ala Leu Phe
85 90 95Gly His Ala Glu Val Arg Cys Ala Asp Asp Gly Asp Leu Ala Ala Leu
100 105 110Ala Glu Ala His Glu Ala Arg Cys Pro Asp Leu Leu Tyr Leu Gly Val
115 120 125Gly Thr Gly Ile Gly Gly Gly Ile Val Leu Asn Gly Lys Pro Val Pro
130 135 140Gly Val Gly Arg Gly Ser Cys Glu Val Gly His Leu Val Val Asp Arg145 150 155 160Asp Gly Pro Leu Cys Asp Cys Gly Arg Arg Gly Cys Val Gln Ala Ala
165 170 175Ala Ser Gly Pro Ala Thr Leu Arg Arg Ala Ala Arg Arg Arg Asp Glu
180 185 190Glu Val Thr Phe Thr Ala Leu Arg Gln Ala Val Arg Gly Gly Lys Pro
195 200 205Trp Ala Val Ala Ser Leu Arg Glu Ser Gly Arg Ala Leu Ala Ala Ala
210 215 220Val Thr Gly Val Cys Glu Leu Leu His Pro Ser Leu Val Leu Ile Gly225 230 235 240Gly Gly Phe Ala Ala Ala Met Pro Glu Leu Val Ala Met Val Ala Glu
245 250 255Arg Thr Ala Glu Leu Gly Arg Pro Gly His Pro Pro Pro Pro Val Arg
260 265 270Pro Ala Arg Leu Gly Gly Leu Ser Ser Leu His Gly Ala Val Leu Leu
275 280 285Ala Arg Gly Leu Pro Asp
290<210>17<211>849<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1)..(849)<223><400>17gtg tca ggc gct gca ctc gga gac atc atg ttc acc atc gga gac ttc 48Val Ser Gly Ala Ala Leu Gly Asp Ile Met Phe Thr Ile Gly Asp Phe1 5 10 15gcc cgg cac ggc cgt gtc tcg gtc cgg atg ctg cgc cac tac gac gcc 96Ala Arg His Gly Arg Val Ser Val Arg Met Leu Arg His Tyr Asp Ala
20 25 30atc gga ctg ctg cgc ccg gcc cat gtc gac ccc gcc acc ggc tac cgc 144Ile Gly Leu Leu Arg Pro Ala His Val Asp Pro Ala Thr Gly Tyr Arg
35 40 45cac tac tcg gcc gcc cag ctc agc cgc ctg aac cgg gtc atc gcg ctc 192His Tyr Ser Ala Ala Gln Leu Ser Arg Leu Asn Arg Val Ile Ala Leu
50 55 60aaa gag ctc ggc ttc acc ctc cag cag gtg cgg gac atc gtg gac gag 240Lys Glu Leu Gly Phe Thr Leu Gln Gln Val Arg Asp Ile Val Asp Glu65 70 75 80aag gtc ggc acc gag gag ctg cgc ggc atg ctg cgg ttg cgc cgg gcc 288Lys Val Gly Thr Glu Glu Leu Arg Gly Met Leu Arg Leu Arg Arg Ala
85 90 95gag ctg gaa gcc acg gtg gaa gcc gtg gcg gca cgg ctg gtg cag gtc 336Glu Leu Glu Ala Thr Val Glu Ala Val Ala Ala Arg Leu Val Gln Val
100 105 110gag gcg agg ctc cgg tcg atc gaa agc gag ggg cac atg ccc acc gac 384Glu Ala Arg Leu Arg Ser Ile Glu Ser Glu Gly His Met Pro Thr Asp
115 120 125gac gtc gtc atc aag agg gtc ccc gcg gtg cgg gtg gcg gag ctc acc 432Asp Val Val Ile Lys Arg Val Pro Ala Val Arg Val Ala Glu Leu Thr
130 135 140gcg acc gcc gcc agc ttc gac ccg cag gac atc agc ccg gtc atc aca 480Ala Thr Ala Ala Ser Phe Asp Pro Gln Asp Ile Ser Pro Val Ile Thr145 150 155 160ccc ctc tac gaa gag ctg ttc cgg cgg ctc gac gct gcg ggc atc acc 528Pro Leu Tyr Glu Glu Leu Phe Arg Arg Leu Asp Ala Ala Gly Ile Thr
165 170 175ccg acg ggc cct ggt gtc gca tac tac gag gac gcc ccg gaa ggc ggc 576Pro Thr Gly Pro Gly Val Ala Tyr Tyr Glu Asp Ala Pro Glu Gly Gly
180 185 190ggc gcc atc agt gtg cac gcc gcc gtc cag gtg tcc gcc ccg tca cgg 624Gly Ala Ile Ser Val His Ala Ala Val Gln Val Ser Ala Pro Ser Arg
195 200 205gac ggc gat gac ctc cgg atc crc gat ctg ccg ccc atc gac cac gcc 672Asp Gly Asp Asp Leu Arg Ile Leu Asp Leu Pro Pro Ile Asp His Ala
210 215 220gcc acc atc gtc cac cgc ggc ccg atg gac gcc gtg gtg ccc acg gcc 720Ala Thr Ile Val His Arg Gly Pro Met Asp Ala Val Val Pro Thr Ala225 230 235 240cag gcc ctg gcc cat tgg att gac ggc aac ggc tac cgg tcg acc ggc 768Gln Ala Leu Ala His Trp Ile Asp Gly Asn Gly Tyr Arg Ser Thr Gly
245 250 255tac ccc cgg gag atc acc ctg gag tgc ccg gag aac cgt gcg gaa tgg 816Tyr Pro Arg Glu Ile Thr Leu Glu Cys Pro Glu Asn Arg Ala Glu Trp
260 265 270gtc acg gaa ctc cag aca ccg gtg gtc cag gtc 849Val Thr Glu Leu Gln Thr Pro Val Val Gln Val
275 280<210>18<211>283<212>PRT<213>Streptomyces hygroscopicus<400>18Val Ser Gly Ala Ala Leu Gly Asp Ile Met Phe Thr Ile Gly Asp Phe1 5 10 15Ala Arg His Gly Arg Val Ser Val Arg Met Leu Arg His Tyr Asp Ala
20 25 30Ile Gly Leu Leu Arg Pro Ala His Val Asp Pro Ala Thr Gly Tyr Arg
35 40 45His Tyr Ser Ala Ala Gln Leu Ser Arg Leu Asn Arg Val Ile Ala Leu
50 55 60Lys Glu Leu Gly Phe Thr Leu Gln Gln Val Arg Asp Ile Val Asp Glu65 70 75 80Lys Val Gly Thr Glu Glu Leu Arg Gly Met Leu Arg Leu Arg Arg Ala
85 90 95Glu Leu Glu Ala Thr Val Glu Ala Val Ala Ala Arg Leu Val Gln Val
100 105 110Glu Ala Arg Leu Arg Ser Ile Glu Ser Glu Gly His Met Pro Thr Asp
115 120 125Asp Val Val Ile Lys Arg Val Pro Ala Val Arg Val Ala Glu Leu Thr
130 135 140Ala Thr Ala Ala Ser Phe Asp Pro Gln Asp Ile Ser Pro Val Ile Thr145 150 155 160Pro Leu Tyr Glu Glu Leu Phe Arg Arg Leu Asp Ala Ala Gly Ile Thr
165 170 175Pro Thr Gly Pro Gly Val Ala Tyr Tyr Glu Asp Ala Pro Glu Gly Gly
180 185 190Gly Ala Ile Ser Val His Ala Ala Val Gln Val Ser Ala Pro Ser Arg
195 200 205Asp Gly Asp Asp Leu Arg Ile Leu Asp Leu Pro Pro Ile Asp His Ala
210 215 220Ala Thr Ile Val His Arg Gly Pro Met Asp Ala Val Val Pro Thr Ala225 230 235 240Gln Ala Leu Ala His Trp Ile Asp Gly Asn Gly Tyr Arg Ser Thr Gly
245 250 255Tyr Pro Arg Glu Ile Thr Leu Glu Cys Pro Glu Asn Arg Ala Glu Trp
260 265 270Val Thr Glu Leu Gln Thr Pro Val Val Gln Val
275 280<210>19<211>2115<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1)..(2115)<223><400>19gtg gct ctg ccg gag gaa cat cgg gtc cag gcg gcg ggg ttc ggg ctt 48Val Ala Leu Pro Glu Glu His Arg Val Gln Ala Ala Gly Phe Gly Leu1 5 10 15cat ccg gca ctc ctc gac gcg gcc atg cac acc atc gcc ttc cac gac 96His Pro Ala Leu Leu Asp Ala Ala Met His Thr Ile Ala Phe His Asp
20 25 30cga gac gaa gcc gat gcg gag ctc gtg ctg ccg ttc gcc tat cga gag 144Arg Asp Glu Ala Asp Ala Glu Leu Val Leu Pro Phe Ala Tyr Arg Glu
35 40 45gtg gcg ttg cat gct tcg ggg gct tcg gcg ctg cgg gta cgc gta aca 192Val Ala Leu His Ala Ser Gly Ala Ser Ala Leu Arg Val Arg Val Thr
50 55 60ccg tcc ggc ccg aac gcc atg acc ctc gac ctg gcc gat ggc tcc ggg 240Pro Ser Gly Pro Asn Ala Met Thr Leu Asp Leu Ala Asp Gly Ser Gly65 70 75 80gcc ccg gtt gcc tcg gtg ggc tcg gtg gtg tcg cgt ccg gtc ggc gcc 288Ala Pro Val Ala Ser Val Gly Ser Val Val Ser Arg Pro Val Gly Ala
85 90 95gag cac ttc ggc acg gtg gcg acg gcg gac cgg atg ttc cgc gtc gca 336Glu His Phe Gly Thr Val Ala Thr Ala Asp Arg Met Phe Arg Val Ala
100 105 110tgg gag gaa ctg ccg att cag ccg gac ggc acg acc gcg gaa ccc gta 384Trp Glu Glu Leu Pro Ile Gln Pro Asp Gly Thr Thr Ala Glu Pro Val
115 120 125ccg gtg gcc gat gcc gag gac gtg cac cgt ctg gtc acg gcg cca gag 432Pro Val Ala Asp Ala Glu Asp Val His Arg Leu Val Thr Ala Pro Glu
130 135 140acc tca ccg ccc gat gtg ctg ttg ctg gac ctg ggc ggt ggc gtc ggc 480Thr Ser Pro Pro Asp Val Leu Leu Leu Asp Leu Gly Gly Gly Val Gly145 150 155 160ggt ggt tcg gcc gac gta cgc gag ctg acc gga cgg gcg ttg cgc gtt 528Gly Gly Ser Ala Asp Val Arg Glu Leu Thr Gly Arg Ala Leu Arg Val
165 170 175gta cag acg tgg ctg gag gag ccc tcg ttg gcg ttg agt cgg ctg gtc 576Val Gln Thr Trp Leu Glu Glu Pro Ser Leu Ala Leu Ser Arg Leu Val
180 185 190gtg gtg acg cgg ggc gcc gtg gcc gtc cgg gag agc gat ccg gtc gat 624Val Val Thr Arg Gly Ala Val Ala Val Arg Glu Ser Asp Pro Val Asp
195 200 205ccg gcg atg gca gcg gta tgg ggg ctg atg gga tcc gca caa gcg gag 672Pro Ala Met Ala Ala Val Trp Gly Leu Met Gly Ser Ala Gln Ala Glu
210 215 220aac ccc ggg cgc atc ctc ctc ctc gac atc gat caa ggg acg ata ccg 720Asn Pro Gly Arg Ile Leu Leu Leu Asp Ile Asp Gln Gly Thr Ile Pro225 230 235 240acc ccg cta ctg ccc gca ctg ctc gtc ggt gac cag cac caa ctg gcc 768Thr Pro Leu Leu Pro Ala Leu Leu Val Gly Asp Gln His Gln Leu Ala
245 250 255cta cgc gac acc acc tgc ttc acc cgc cac ctc atc cgt gtg ctg gat 816Leu Arg Asp Thr Thr Cys Phe Thr Arg His Leu Ile Arg Val Leu Asp
260 265 270gcg ccg cag tcc ggt ccg ggt ggt ttg gag gac gtg ggt ggg acg gta 864Ala Pro Gln Ser Gly Pro Gly Gly Leu Glu Asp Val Gly Gly Thr Val
275 280 285ctg gtg acg ggt ggg acg ggg gcg ttg ggt gcg gtg gtg gca cgg cat 912Leu Val Thr Gly Gly Thr Gly Ala Leu Gly Ala Val Val Ala Arg His
290 295 300ctg gtg gcg gtg cac ggg atg cgg agt gtg gtg ttg gcg agc cgg aat 960Leu Val Ala Val His Gly Met Arg Ser Val Val Leu Ala Ser Arg Asn305 310 315 320ggg ctt gag gca ccc ggc gcc gcc gag ttg gag gcg gag ctg gtg aag 1008Gly Leu Glu Ala Pro Gly Ala Ala Glu Leu Glu Ala Glu Leu Val Lys
325 330 335gcg ggt gcg cgc gta cgc atc gtc gcg tgt gat gtg gcg gac cgg gac 1056Ala Gly Ala Arg Val Arg Ile Val Ala Cys Asp Val Ala Asp Arg Asp
340 345 350gcg gtg gcc ggg ctg ctg gac gcc gtc ccg gca gac gct ccg ttg tcg 1104Ala Val Ala Gly Leu Leu Asp Ala Val Pro Ala Asp Ala Pro Leu Ser
355 360 365gcg gtg gtg cat acg gcc ggt gtt ctg gat gac ggt gtg ctg acg gcg 1152Ala Val Val His Thr Ala Gly Val Leu Asp Asp Gly Val Leu Thr Ala
370 375 380ttg acc ccg gaa cgt atg gac gcg gtg ctc cgg ccg aag gtg gac ggc 1200Leu Thr Pro Glu Arg Met Asp Ala Val Leu Arg Pro Lys Val Asp Gly385 390 395 400gca ctc cat ctc cac gag ctg acc cgg cac ctg ggc ctg tcc gcc ttc 1248Ala Leu His Leu His Glu Leu Thr Arg His Leu Gly Leu Ser Ala Phe
405 410 415gtc ctg ttc tcc tcc gcc gcc ggc acc ctc ggc aac gcg ggt cag ggc 1296Val Leu Phe Ser Ser Ala Ala Gly Thr Leu Gly Asn Ala Gly Gln Gly
420 425 430aac tac gcc gcc gcc aac gcc tac ctc gac gcg ctg gcc cat cga cgc 1344Asn Tyr Ala Ala Ala Asn Ala Tyr Leu Asp Ala Leu Ala His Arg Arg
435 440 445cgg gcc cag ggg ctg ccg gca gta tcc ctc gcc tgg ggc atg tgg cag 1392Arg Ala Gln Gly Leu Pro Ala Val Ser Leu Ala Trp Gly Met Trp Gln
450 455 460cag gcc gcg gga acg ggg atg acc ggc cgt ctc ggc gat gcc gag cag 1440Gln Ala Ala Gly Thr Gly Met Thr Gly Arg Leu Gly Asp Ala Glu Gln465 470 475 480cgc cgg atg aca cgc ggc ggg gtg gcc ccc ttg tcc ccg gcc gag ggc 1488Arg Arg Met Thr Arg Gly Gly Val Ala Pro Leu Ser Pro Ala Glu Gly
485 490 495atg gag ctc ttc gac act gcg ctg cgt atg gcc gaa ccc acg gtc ctc 1536Met Glu Leu Phe Asp Thr Ala Leu Arg Met Ala Glu Pro Thr Val Leu
500 505 510ccc atc aaa ctg gac ctc ggt gcg ctc cgc gcc cag gcc gcc acc ggg 1584Pro Ile Lys Leu Asp Leu Gly Ala Leu Arg Ala Gln Ala Ala Thr Gly
515 520 525gcg gtg cag ccg ttg ctg cac cgg ctg gtg cca ccg gtc cgc cga gcc 1632Ala Val Gln Pro Leu Leu His Arg Leu Val Pro Pro Val Arg Arg Ala
530 535 540act cgc gcc acg gcc gag cag ggc ctg gtg acc ggc cgg ctg gcg ggc 1680Thr Arg Ala Thr Ala Glu Gln Gly Leu Val Thr Gly Arg Leu Ala Gly545 550 555 560gcg acc ccc gag gag cgg gag cgg atc ctg ctg gag atg gtc cag cag 1728Ala Thr Pro Glu Glu Arg Glu Arg Ile Leu Leu Glu Met Val Gln Gln
565 570 575gag gcc gcc cgg gtc ctg gga cac tcg gcg gct gcc acg ctc gac ccc 1776Glu Ala Ala Arg Val Leu Gly His Ser Ala Ala Ala Thr Leu Asp Pro
580 585 590gat gtg ctg ttc acc gag atc ggc ctg gac tcc ctg atg gcg gtg gaa 1824Asp Val Leu Phe Thr Glu Ile Gly Leu Asp Ser Leu Met Ala Val Glu
595 600 605cta cgc gat cgc ctg gcc aag cgc acc gcg ctg cgg ttg cct ccc agc 1872Leu Arg Asp Arg Leu Ala Lys Arg Thr Ala Leu Arg Leu Pro Pro Ser
610 615 620ttt gtc ttc gac cac ccc acc ctc cgg atg ctg gcc cgg cag ctg tgg 1920Phe Val Phe Asp His Pro Thr Leu Arg Met Leu Ala Arg Gln Leu Trp625 630 635 640gac gag ctg gag aaa gcc gat acg gac gct ccc gcc gca tcc gcc ccg 1968Asp Glu Leu Glu Lys Ala Asp Thr Asp Ala Pro Ala Ala Ser Ala Pro
645 650 655act ccc gca tcc gcc gaa act ccc gca tcc gcc cca acc gcc gga gcc 2016Thr Pro Ala Ser Ala Glu Thr Pro Ala Ser Ala Pro Thr Ala Gly Ala
660 665 670acc ccg tca ccc gga gcc acc ccg tca ccc gga gcc acc ccg tca ccc 2064Thr Pro Ser Pro Gly Ala Thr Pro Ser Pro Gly Ala Thr Pro Ser Pro
675 680 685gga gcc act ctg cca ccc gcg ccc acc ccg cct tcc gga gcc gcc cag 2112Gly Ala Thr Leu Pro Pro Ala Pro Thr Pro Pro Ser Gly Ala Ala Gln
690 695 700gag 2115Glu705<210>20<211>705<212>PRT<213>Streptomyces hygroscopicus<400>20Val Ala Leu Pro Glu Glu His Arg Val Gln Ala Ala Gly Phe Gly Leu1 5 10 15His Pro Ala Leu Leu Asp Ala Ala Met His Thr Ile Ala Phe His Asp
20 25 30Arg Asp Glu Ala Asp Ala Glu Leu Val Leu Pro Phe Ala Tyr Arg Glu
35 40 45Val Ala Leu His Ala Ser Gly Ala Ser Ala Leu Arg Val Arg Val Thr
50 55 60Pro Ser Gly Pro Asn Ala Met Thr Leu Asp Leu Ala Asp Gly Ser Gly65 70 75 80Ala Pro Val Ala Ser Val Gly Ser Val Val Ser Arg Pro Val Gly Ala
85 90 95Glu His Phe Gly Thr Val Ala Thr Ala Asp Arg Met Phe Arg Val Ala
100 105 110Trp Glu Glu Leu Pro Ile Gln Pro Asp Gly Thr Thr Ala Glu Pro Val
115 120 125Pro Val Ala Asp Ala Glu Asp Val His Arg Leu Val Thr Ala Pro Glu
130 135 140Thr Ser Pro Pro Asp Val Leu Leu Leu Asp Leu Gly Gly Gly Val Gly145 150 155 160Gly Gly Ser Ala Asp Val Arg Glu Leu Thr Gly Arg Ala Leu Arg Val
165 170 175Val Gln Thr Trp Leu Glu Glu Pro Ser Leu Ala Leu Ser Arg Leu Val
180 185 190Val Val Thr Arg Gly Ala Val Ala Val Arg Glu Ser Asp Pro Val Asp
195 200 205Pro Ala Met Ala Ala Val Trp Gly Leu Met Gly Ser Ala Gln Ala Glu
210 215 220Asn Pro Gly Arg Ile Leu Leu Leu Asp Ile Asp Gln Gly Thr Ile Pro225 230 235 240Thr Pro Leu Leu Pro Ala Leu Leu Val Gly Asp Gln His Gln Leu Ala
245 250 255Leu Arg Asp Thr Thr Cys Phe Thr Arg His Leu Ile Arg Val Leu Asp
260 265 270Ala Pro Gln Ser Gly Pro Gly Gly Leu Glu Asp Val Gly Gly Thr Val
275 280 285Leu Val Thr Gly Gly Thr Gly Ala Leu Gly Ala Val Val Ala Arg His
290 295 300Leu Val Ala Val His Gly Met Arg Ser Val Val Leu Ala Ser Arg Asn305 310 315 320Gly Leu Glu Ala Pro Gly Ala Ala Glu Leu Glu Ala Glu Leu Val Lys
325 330 335Ala Gly Ala Arg Val Arg Ile Val Ala Cys Asp Val Ala Asp Arg Asp
340 345 350Ala Val Ala Gly Leu Leu Asp Ala Val Pro Ala Asp Ala Pro Leu Ser
355 360 365Ala Val Val His Thr Ala Gly Val Leu Asp Asp Gly Val Leu Thr Ala
370 375 380Leu Thr Pro Glu Arg Met Asp Ala Val Leu Arg Pro Lys Val Asp Gly385 390 395 400Ala Leu His Leu His Glu Leu Thr Arg His Leu Gly Leu Ser Ala Phe
405 410 415Val Leu Phe Ser Ser Ala Ala Gly Thr Leu Gly Asn Ala Gly Gln Gly
420 425 430Asn Tyr Ala Ala Ala Asn Ala Tyr Leu Asp Ala Leu Ala His Arg Arg
435 440 445Arg Ala Gln Gly Leu Pro Ala Val Ser Leu Ala Trp Gly Met Trp Gln
450 455 460Gln Ala Ala Gly Thr Gly Met Thr Gly Arg Leu Gly Asp Ala Glu Gln465 470 475 480Arg Arg Met Thr Arg Gly Gly Val Ala Pro Leu Ser Pro Ala Glu Gly
485 490 495Met Glu Leu Phe Asp Thr Ala Leu Arg Met Ala Glu Pro Thr Val Leu
500 505 510Pro Ile Lys Leu Asp Leu Gly Ala Leu Arg Ala Gln Ala Ala Thr Gly
515 520 525Ala Val Gln Pro Leu Leu His Arg Leu Val Pro Pro Val Arg Arg Ala
530 535 540Thr Arg Ala Thr Ala Glu Gln Gly Leu Val Thr Gly Arg Leu Ala Gly545 550 555 560Ala Thr Pro Glu Glu Arg Glu Arg Ile Leu Leu Glu Met Val Gln Gln
565 570 575Glu Ala Ala Arg Val Leu Gly His Ser Ala Ala Ala Thr Leu Asp Pro
580 585 590Asp Val Leu Phe Thr Glu Ile Gly Leu Asp Ser Leu Met Ala Val Glu
595 600 605Leu Arg Asp Arg Leu Ala Lys Arg Thr Ala Leu Arg Leu Pro Pro Ser
610 615 620Phe Val Phe Asp His Pro Thr Leu Arg Met Leu Ala Arg Gln Leu Trp625 630 635 640Asp Glu Leu Glu Lys Ala Asp Thr Asp Ala Pro Ala Ala Ser Ala Pro
645 650 655Thr Pro Ala Ser Ala Glu Thr Pro Ala Ser Ala Pro Thr Ala Gly Ala
660 665 670Thr Pro Ser Pro Gly Ala Thr Pro Ser Pro Gly Ala Thr Pro Ser Pro
675 680 685Gly Ala Thr Leu Pro Pro Ala Pro Thr Pro Pro Ser Gly Ala Ala Gln
690 695 700Glu705<210>21<211>1026<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1)..(1026)<223><400>21gtg gga cga gct gga gaa agc cga tac gga cgc tcc cgc cgc atc cgc 48Val Gly Arg Ala Gly Glu Ser Arg Tyr Gly Arg Ser Arg Arg Ile Arg1 5 10 15ccc gac tcc cgc atc cgc cga aac tcc cgc atc cgc ccc aac cgc cgg 96Pro Asp Ser Arg Ile Arg Arg Asn Ser Arg Ile Arg Pro Asn Arg Arg
20 25 30agc cac ccc gtc acc cgg agc cac ccc gtc acc cgg agc cac ccc gtc 144Ser His Pro Val Thr Arg Ser His Pro Val Thr Arg Ser His Pro Val
35 40 45acc cgg agc cac tct gcc acc cgc gcc cac ccc gcc ttc cgg agc cgc 192Thr Arg Ser His Ser Ala Thr Arg Ala His Pro Ala Phe Arg Ser Arg
50 55 60cca gga gtg agc cct gcc cag ccc agc aca act cca ctc ggc aga ccg 240Pro Gly Val Ser Pro Ala Gln Pro Ser Thr Thr Pro Leu Gly Arg Pro65 70 75 80gca ccg acc gaa gcg gca aga aag gga tcg cac atg ttc agc acg gac 288Ala Pro Thr Glu Ala Ala Arg Lys Gly Ser His Met Phe Ser Thr Asp
85 90 95acg tac ctg gcg cat ctg ggg ttt ccc cag ccg ccc gcc ccc acc ctg 336Thr Tyr Leu Ala His Leu Gly Phe Pro Gln Pro Pro Ala Pro Thr Leu
100 105 110ccg aac ctc cgg cag ttg cac cgc ggc cat ctg atg gcg gtc cct tac 384Pro Asn Leu Arg Gln Leu His Arg Gly His Leu Met Ala Val Pro Tyr
115 120 125gac acc aac cac acc cac cgc ctc agc gcg gag aac atg gcc gac atc 432Asp Thr Asn His Thr His Arg Leu Ser Ala Glu Asn Met Ala Asp Ile
130 135 140gat atc gac aag gca ttc gag gcc atc gtg ccg acc ggc gcc ggt ggc 480Asp Ile Asp Lys Ala Phe Glu Ala Ile Val Pro Thr Gly Ala Gly Gly145 150 155 160atg tgc ctg gag ctg aac acc ctg ttc gcc cag ttg ctc cgc gag ctg 528Met Cys Leu Glu Leu Asn Thr Leu Phe Ala Gln Leu Leu Arg Glu Leu
165 170 175ggc tat gac ctg gac gtc atc agc gga ggc acg tat ctg ccc ggt gac 576Gly Tyr Asp Leu Asp Val Ile Ser Gly Gly Thr Tyr Leu Pro Gly Asp
180 185 190atc ttc gcc ccc gac ccc gag cac atg ctg atg ctc gtc cgt atc gac 624Ile Phe Ala Pro Asp Pro Glu His Met Leu Met Leu Val Arg Ile Asp
195 200 205ggg cag gag tgg ctg gcc gat gtg ggg cac gcc ggt ctc tgt ttc acc 672Gly Gln Glu Trp Leu Ala Asp Val Gly His Ala Gly Leu Cys Phe Thr
210 215 220gag ccg ctg cgc ctg tcc gag gag gtg cag tgg cag tac ggc tgc gct 720Glu Pro Leu Arg Leu Ser Glu Glu Val Gln Trp Gln Tyr Gly Cys Ala225 230 235 240ttc cgg ctg atc cgg cgg gat ggc tat ctc gtg ctc cag gcc aag acc 768Phe Arg Leu Ile Arg Arg Asp Gly Tyr Leu Val Leu Gln Ala Lys Thr
245 250 255ctg gac cac gac tgg cgc acc acc tac cgc ttc acc acc gag ccc agg 816Leu Asp His Asp Trp Arg Thr Thr Tyr Arg Phe Thr Thr Glu Pro Arg
260 265 270acc tat gac gcc tgg gcc ggg gtc ggt gag ggc aat ggc ccg gcc atc 864Thr Tyr Asp Ala Trp Ala Gly Val Gly Glu Gly Asn Gly Pro Ala Ile
275 280 285ctg gcg gcg atg cgc cga cgc agg cgc gcc atc gac aag ggg cag gtc 912Leu Ala Ala Met Arg Arg Arg Arg Arg Ala Ile Asp Lys Gly Gln Val
290 295 300ttc ctc acc aac aac atg ttc acg atc gtg gag aac ggc cat gag aag 960Phe Leu Thr Asn Asn Met Phe Thr Ile Val Glu Asn Gly His Glu Lys305 310 315 320gtc acc ctc ctc gtc gat ccg gaa cgg cgc gcc cag gtg ctc gac acg 1008Val Thr Leu Leu Val Asp Pro Glu Arg Arg Ala Gln Val Leu Asp Thr
325 330 335tac tgg gac ggt cgc gac 1026Tyr Trp Asp Gly Arg Asp
340<210>22<211>342<212>PRT<213>Streptomyces hygroscopicus<400>22Val Gly Arg Ala Gly Glu Ser Arg Tyr Gly Arg Ser Arg Arg Ile Arg1 5 10 15Pro Asp Ser Arg Ile Arg Arg Asn Ser Arg Ile Arg Pro Asn Arg Arg
20 25 30Ser His Pro Val Thr Arg Ser His Pro Val Thr Arg Ser His Pro Val
35 40 45Thr Arg Ser His Ser Ala Thr Arg Ala His Pro Ala Phe Arg Ser Arg
50 55 60Pro Gly Val Ser Pro Ala Gln Pro Ser Thr Thr Pro Leu Gly Arg Pro65 70 75 80Ala Pro Thr Glu Ala Ala Arg Lys Gly Ser His Met Phe Ser Thr Asp
85 90 95Thr Tyr Leu Ala His Leu Gly Phe Pro Gln Pro Pro Ala Pro Thr Leu
100 105 110Pro Asn Leu Arg Gln Leu His Arg Gly His Leu Met Ala Val Pro Tyr
115 120 125Asp Thr Asn His Thr His Arg Leu Ser Ala Glu Asn Met Ala Asp Ile
130 135 140Asp Ile Asp Lys Ala Phe Glu Ala Ile Val Pro Thr Gly Ala Gly Gly145 150 155 160Met Cys Leu Glu Leu Asn Thr Leu Phe Ala Gln Leu Leu Arg Glu Leu
165 170 175Gly Tyr Asp Leu Asp Val Ile Ser Gly Gly Thr Tyr Leu Pro Gly Asp
180 185 190Ile Phe Ala Pro Asp Pro Glu His Met Leu Met Leu Val Arg Ile Asp
195 200 205Gly Gln Glu Trp Leu Ala Asp Val Gly His Ala Gly Leu Cys Phe Thr
210 215 220Glu Pro Leu Arg Leu Ser Glu Glu Val Gln Trp Gln Tyr Gly Cys Ala225 230 235 240Phe Arg Leu Ile Arg Arg Asp Gly Tyr Leu Val Leu Gln Ala Lys Thr
245 250 255Leu Asp His Asp Trp Arg Thr Thr Tyr Arg Phe Thr Thr Glu Pro Arg
260 265 270Thr Tyr Asp Ala Trp Ala Gly Val Gly Glu Gly Asn Gly Pro Ala Ile
275 280 285Leu Ala Ala Met Arg Arg Arg Arg Arg Ala Ile Asp Lys Gly Gln Val
290 295 300Phe Leu Thr Asn Asn Met Phe Thr Ile Val Glu Asn Gly His Glu Lys305 310 315 320Val Thr Leu Leu Val Asp Pro Glu Arg Arg Ala Gln Val Leu Asp Thr
325 330 335Tyr Trp Asp Gly Arg Asp
340<210>23<211>1224<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1)..(1224)<223><400>23atg aaa ttc ggt ttg ctg tac ggg gcg cag ctg ccc cga ccc tgg acc 48Met Lys Phe Gly Leu Leu Tyr Gly Ala Gln Leu Pro Arg Pro Trp Thr1 5 10 15cag gac tcc gaa cac cgc ctg ttc aac gag atg ttg gac gag atc gag 96Gln Asp Ser Glu His Arg Leu Phe Asn Glu Met Leu Asp Glu Ile Glu
20 25 30ctg gcc gac cgg ctg ggc ttc gac cat gtg tgg tgt cct gag cac cac 144Leu Ala Asp Arg Leu Gly Phe Asp His Val Trp Cys Pro Glu His His
35 40 45ttc ctg gag gag tac tcg cac atg tcc gcg ccg gag gcg ttc ctc ggc 192Phe Leu Glu Glu Tyr Ser His Met Ser Ala Pro Glu Ala Phe Leu Gly
50 55 60gcg gtc agc cag cgc acc agc cgc atc cgc atc ggc cac gcg gtg gcc 240Ala Val Ser Gln Arg Thr Ser Arg Ile Arg Ile Gly His Ala Val Ala65 70 75 80ctg atg cct ccg gcg ttc aat ccg acg gca cgg gtc gcc gag cgg atc 288Leu Met Pro Pro Ala Phe Asn Pro Thr Ala Arg Val Ala Glu Arg Ile
85 90 95gcc acg ctg gac ctg ctc tcc gac ggc cgg gtg gac ttc ggc acg ggc 336Ala Thr Leu Asp Leu Leu Ser Asp Gly Arg Val Asp Phe Gly Thr Gly
100 105 110gag tcc acc acc ccc acc gag ctg ggc gga ttc ggc gtg gag cgc tcc 384Glu Ser Thr Thr Pro Thr Glu Leu Gly Gly Phe Gly Val Glu Arg Ser
115 120 125gtg aaa cgg gac cag tgg gcg gag gcg gtg gac gcc gtc gcc cgg atg 432Val Lys Arg Asp Gln Trp Ala Glu Ala Val Asp Ala Val Ala Arg Met
130 135 140ttc gtc gag gag ccc ttc gcc gga tac gag ggc aag tac gtg tcc gcc 480Phe Val Glu Glu Pro Phe Ala Gly Tyr Glu Gly Lys Tyr Val Ser Ala145 150 155 160ccg atc cgc aat gtg ctg ccc aag acc cgg cag aag cca cat ccg ccg 528Pro Ile Arg ASn Val Leu Pro Lys Thr Arg Gln Lys Pro His Pro Pro
165 170 175atg tgg atg gcc tgc ggg aac cgg gac gcg atc cgc acc gcg gcc gcc 576Met Trp Met Ala Cys Gly Asn Arg Asp Ala Ile Arg Thr Ala Ala Ala
180 185 190aag ggg ctc ggc gcg ctg aac ttc tcc ttc ttc ggg ccg gcg gag acc 624Lys Gly Leu Gly Ala Leu Asn Phe Ser Phe Phe Gly Pro Ala Glu Thr
195 200 205aag aag tgg gtc gac gcc tac tac tcg ggc atc gaa tcg gcg gac tgt 672Lys Lys Trp Val Asp Ala Tyr Tyr Ser Gly Ile Glu Ser Ala Asp Cys
210 215 220gtg ccc gcc gcg ttc gcc gtc aac gca cag atc gcc gcg acc atc ccg 720Val Pro Ala Ala Phe Ala Val Asn Ala Gln Ile Ala Ala Thr Ile Pro225 230 235 240atg ttc tgc cac cgg gac gag acc acg gcc gtg gaa cgc gcc gtc gac 768Met Phe Cys His Arg Asp Glu Thr Thr Ala Val Glu Arg Ala Val Asp
245 250 255ggc gtc cag ttc ttc aac ttc ggc ctc ggc ttc tac gcg ggc ttc ggc 816Gly Val Gln Phe Phe Asn Phe Gly Leu Gly Phe Tyr Ala Gly Phe Gly
260 265 270acc gcc gca ccg gcc cgc acc cgg ctg tgg gag gag ttc cag cgc gac 864Thr Ala Ala Pro Ala Arg Thr Arg Leu Trp Glu Glu Phe Gln Arg Asp
275 280 285cgc gac aag cag ggc atg ggc cgc tcc tcc ttc ggc aag ccc gga atg 912Arg Asp Lys Gln Gly Met Gly Arg Ser Ser Phe Gly Lys Pro Gly Met
290 295 300ccg ctg ggc aat ccg gcc cgg ggc gcg gtg ggc act ccg cac cag ata 960Pro Leu Gly Asn Pro Ala Arg Gly Ala Val Gly Thr Pro His Gln Ile305 310 315 320cgc gac ttc ctg cgg ctg cac gag gag gcc gga ctg gac cag gcg atc 1008Arg Asp Phe Leu Arg Leu His Glu Glu Ala Gly Leu Asp Gln Ala Ile
325 330 335ttc ctc gtc cag ggc ggt ggc acc cgg cac gag cac atc cgc gaa tcg 1056Phe Leu Val Gln Gly Gly Gly Thr Arg His Glu His Ile Arg Glu Ser
340 345 350ctg gag ctc ttc gcc aat gag gtg atg ccc gag ttc aag gag cgc gat 1104Leu Glu Leu Phe Ala Asn Glu Val Met Pro Glu Phe Lys Glu Arg Asp
355 360 365gag gcc gcc gta cgg ctg aag acg gcc cgg ctc cag ccc gcc atc gac 1152Glu Ala Ala Val Arg Leu Lys Thr Ala Arg Leu Gln Pro Ala Ile Asp
370 375 380gcc gcc atg gcc cgg cgc gag ccg cca cgg acg gcc gac ccc gac tac 1200Ala Ala Met Ala Arg Arg Glu Pro Pro Arg Thr Ala Asp Pro Asp Tyr385 390 395 400atc atc ccc gcc cgg agc cag agc 1224Ile Ile Pro Ala Arg Ser Gln Ser
405<210>24<211>408<212>PRT<213>Streptomyces hygroscopicus<400>24Met Lys Phe Gly Leu Leu Tyr Gly Ala Gln Leu Pro Arg Pro Trp Thr1 5 10 15Gln Asp Ser Glu His Arg Leu Phe Asn Glu Met Leu Asp Glu Ile Glu
20 25 30Leu Ala Asp Arg Leu Gly Phe Asp His Val Trp Cys Pro Glu His His
35 40 45Phe Leu Glu Glu Tyr Ser His Met Ser Ala Pro Glu Ala Phe Leu Gly
50 55 60Ala Val Ser Gln Arg Thr Ser Arg Ile Arg Ile Gly His Ala Val Ala65 70 75 80Leu Met Pro Pro Ala Phe Asn Pro Thr Ala Arg Val Ala Glu Arg Ile
85 90 95Ala Thr Leu Asp Leu Leu Ser Asp Gly Arg Val Asp Phe Gly Thr Gly
100 105 110Glu Ser Thr Thr Pro Thr Glu Leu Gly Gly Phe Gly Val Glu Arg Ser
115 120 125Val Lys Arg Asp Gln Trp Ala Glu Ala Val Asp Ala Val Ala Arg Met
130 135 140Phe Val Glu Glu Pro Phe Ala Gly Tyr Glu Gly Lys Tyr Val Ser Ala145 150 155 160Pro Ile Arg Asn Val Leu Pro Lys Thr Arg Gln Lys Pro His Pro Pro
165 170 175Met Trp Met Ala Cys Gly Asn Arg Asp Ala Ile Arg Thr Ala Ala Ala
180 185 190Lys Gly Leu Gly Ala Leu Asn Phe Ser Phe Phe Gly Pro Ala Glu Thr
195 200 205Lys Lys Trp Val Asp Ala Tyr Tyr Ser Gly Ile Glu Ser Ala Asp Cys
210 215 220Val Pro Ala Ala Phe Ala Val Asn Ala Gln Ile Ala Ala Thr Ile Pro225 230 235 240Met Phe Cys His Arg Asp Glu Thr Thr Ala Val Glu Arg Ala Val Asp
245 250 255Gly Val Gln Phe Phe Asn Phe Gly Leu Gly Phe Tyr Ala Gly Phe Gly
260 265 270Thr Ala Ala Pro Ala Arg Thr Arg Leu Trp Glu Glu Phe Gln Arg Asp
275 280 285Arg Asp Lys Gln Gly Met Gly Arg Ser Ser Phe Gly Lys Pro Gly Met
290 295 300Pro Leu Gly Asn Pro Ala Arg Gly Ala Val Gly Thr Pro His Gln Ile305 310 315 320Arg Asp Phe Leu Arg Leu His Glu Glu Ala Gly Leu Asp Gln Ala Ile
325 330 335Phe Leu Val Gln Gly Gly Gly Thr Arg His Glu His Ile Arg Glu Ser
340 345 350Leu Glu Leu Phe Ala Asn Glu Val Met Pro Glu Phe Lys Glu Arg Asp
355 360 365Glu Ala Ala Val Arg Leu Lys Thr Ala Arg Leu Gln Pro Ala Ile Asp
370 375 380Ala Ala Met Ala Arg Arg Glu Pro Pro Arg Thr Ala Asp Pro Asp Tyr385 390 395 400Ile Ile Pro Ala Arg Ser Gln Ser
405<210>25<211>1239<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1)..(1239)<223><400>25gtg tcc ggg cgc cacttc gaa caa gga gaa cgt ggt acc gcc atg gct 48Val Ser Gly Arg His Phe Glu Gln Gly Glu Arg Gly Thr Ala Met Ala1 5 10 15gac acc ccc gaa gaa gaa ctc cgc atc ctc gac ccg cag tcc gtc gcg 96Asp Thr Pro Glu Glu Glu Leu Arg Ile Leu Asp Pro Gln Ser Val Ala
20 25 30cag gag ctg cgc aag cac ggc ccg cct cgg cag atc acg atg cac ggc 144Gln Glu Leu Arg Lys His Gly Pro Pro Arg Gln Ile Thr Met His Gly
35 40 45acc acg gcg tgg ctc gtc tcc cgg tac gag gag gtc cgg gac tgt ctc 192Thr Thr Ala Trp Leu Val Ser Arg Tyr Glu Glu Val Arg Asp Cys Leu
50 55 60gga cac ccc gga atg agc ccg gcc gcc gcc tac gcc gcc tcc cag ggc 240Gly His Pro Gly Met Ser Pro Ala Ala Ala Tyr Ala Ala Ser Gln Gly65 70 75 80cag acc aat ccg gtg agc ggg ttg ttc gag gac acg gtg gcc ggt acc 288Gln Thr Asn Pro Val Ser Gly Leu Phe Glu Asp Thr Val Ala Gly Thr
85 90 95aat ccg ccc cag cac acc cgg ctg cgc agg ctg ctg gcc aag gcg ttc 336Asn Pro Pro Gln His Thr Arg Leu Arg Arg Leu Leu Ala Lys Ala Phe
100 105 110acg gta cgc aga gtg gag agt ctg cgg cca cgg gtg cag gag atc acc 384Thr Val Arg Arg Val Glu Ser Leu Arg Pro Arg Val Gln Glu Ile Thr
115 120 125gac aca ctg ctg gac cgg atc gcc gtc gac ggc cgg gcc gac ctc gtc 432Asp Thr Leu Leu Asp Arg Ile Ala Val Asp Gly Arg Ala Asp Leu Val
130 135 140agc gcg ctg gcc att ccg ctg ccc atg cag gtg atc tgc gaa ctc ctc 480Ser Ala Leu Ala Ile Pro Leu Pro Met Gln Val Ile Cys Glu Leu Leu145 150 155 160ggt gtg ccc atc gcc gac cgc acc gaa ttc cac cag tgg gcc gat ctg 528Gly Val Pro Ile Ala Asp Arg Thr Glu Phe His Gln Trp Ala Asp Leu
165 170 175atg ctc acg ccc ccg ctg gac ccg gac acc gcc gcg cgt tcc cag gac 576Met Leu Thr Pro Pro Leu Asp Pro Asp Thr Ala Ala Arg Ser Gln Asp
180 185 190gcc tcc gcc aag ctg tgg acg tat atg gag gac ctc gcc gag gcc agg 624Ala Ser Ala Lys Leu Trp Thr Tyr Met Glu Asp Leu Ala Glu Ala Arg
195 200 205cgg aag gcc ccg gag gac gac ctg atc agc gat ctg atg tcc gca cac 672Arg Lys Ala Pro Glu Asp Asp Leu Ile Ser Asp Leu Met Ser Ala His
210 215 220gag gac gac cgg ctc agc cac cgc gag gtg gtc gcc acc gcc cgg atg 720Glu Asp Asp Arg Leu Ser His Arg Glu Val Val Ala Thr Ala Arg Met225 230 235 240atg ctg atc gcg ggg tac gag ctg acc ggc agc ttc atc agc aac gcg 768Met Leu Ile Ala Gly Tyr Glu Leu Thr Gly Ser Phe Ile Ser Asn Ala
245 250 255gtt ttc tcg ctg ctg tcc cag ccc gac cag atg gaa ctg ctg cgc aag 816Val Phe Ser Leu Leu Ser Gln Pro Asp Gln Met Glu Leu Leu Arg Lys
260 265 270gac ccc gag ctg gcc ggg cgc ggt ctg gag gag ctg ctc cgg cac gcc 864Asp Pro Glu Leu Ala Gly Arg Gly Leu Glu Glu Leu Leu Arg His Ala
275 280 285ggg ccg ggc att ctc atc gtg cgt ttc gcc aac gag gac gtg gag atc 912Gly Pro Gly Ile Leu Ile Val Arg Phe Ala Asn Glu Asp Val Glu Ile
290 295 300ggc tcc gta tcc atc cgc gcc ggc gac cag gtg ctc ctg gac atg gac 960Gly Ser Val Ser Ile Arg Ala Gly Asp Gln Val Leu Leu Asp Met Asp305 310 315 320gcc gca cac tcc gac ccg gcg cac ttc acc gac ggc gag cgg ctg gac 1008Ala Ala His Ser Asp Pro Ala His Phe Thr Asp Gly Glu Arg Leu Asp
325 330 335ctc acg agg gac tcg gcc gta cac ctc cag ttc ggc cat ggc atc cac 1056Leu Thr Arg Asp Ser Ala Val His Leu Gln Phe Gly His Gly Ile His
340 345 350tac tgc atc ggc gcg ccg ctg gcc agg gtg gag ggg cag atc gcc ctg 1104Tyr Cys Ile Gly Ala Pro Leu Ala Arg Val Glu Gly Gln Ile Ala Leu
355 360 365gag agc ctg gtg cgg cgg ttc ccc ggg ctt cgg ctg agc gtt ccc gcc 1152Glu Ser Leu Val Arg Arg Phe Pro Gly Leu Arg Leu Ser Val Pro Ala
370 375 380gcc gag atc agc cat agc aag aac ccg ttc atc cgc tcg ctg acc gcg 1200Ala Glu Ile Ser His Ser Lys Asn Pro Phe Ile Arg Ser Leu Thr Ala385 390 395 400ctg ccc gtc gag ttc gag gct cag cag ccc gta gcg ggg 1239Leu Pro Val Glu Phe Glu Ala Gln Gln Pro Val Ala Gly
405 410<210>26<211>413<212>PRT<213>Streptomyces hygroscopicus<400>26Val Ser Gly Arg His Phe Glu Gln Gly Glu Arg Gly Thr Ala Met Ala1 5 10 15Asp Thr Pro Glu Glu Glu Leu Arg Ile Leu Asp Pro Gln Ser Val Ala
20 25 30Gln Glu Leu Arg Lys His Gly Pro Pro Arg Gln Ile Thr Met His Gly
35 40 45Thr Thr Ala Trp Leu Val Ser Arg Tyr Glu Glu Val Arg Asp Cys Leu
50 55 60Gly His Pro Gly Met Ser Pro Ala Ala Ala Tyr Ala Ala Ser Gln Gly65 70 75 80Gln Thr Asn Pro Val Ser Gly Leu Phe Glu Asp Thr Val Ala Gly Thr
85 90 95Asn Pro Pro Gln His Thr Arg Leu Arg Arg Leu Leu Ala Lys Ala Phe
100 105 110Thr Val Arg Arg Val Glu Ser Leu Arg Pro Arg Val Gln Glu Ile Thr
115 120 125Asp Thr Leu Leu Asp Arg Ile Ala Val Asp Gly Arg Ala Asp Leu Val
130 135 140Ser Ala Leu Ala Ile Pro Leu Pro Met Gln Val Ile Cys Glu Leu Leu145 150 155 160Gly Val Pro Ile Ala Asp Arg Thr Glu Phe His Gln Trp Ala Asp Leu
165 170 175Met Leu Thr Pro Pro Leu Asp Pro Asp Thr Ala Ala Arg Ser Gln Asp
180 185 190Ala Ser Ala Lys Leu Trp Thr Tyr Met Glu Asp Leu Ala Glu Ala Arg
195 200 205Arg Lys Ala Pro Glu Asp Asp Leu Ile Ser Asp Leu Met Ser Ala His
210 215 220Glu Asp Asp Arg Leu Ser His Arg Glu Val Val Ala Thr Ala Arg Met225 230 235 240Met Leu Ile Ala Gly Tyr Glu Leu Thr Gly Ser Phe Ile Ser Asn Ala
245 250 255Val Phe Ser Leu Leu Ser Gln Pro Asp Gln Met Glu Leu Leu Arg Lys
260 265 270Asp Pro Glu Leu Ala Gly Arg Gly Leu Glu Glu Leu Leu Arg His Ala
275 280 285Gly Pro Gly Ile Leu Ile Val Arg Phe Ala Asn Glu Asp Val Glu Ile
290 295 300Gly Ser Val Ser Ile Arg Ala Gly Asp Gln Val Leu Leu Asp Met Asp305 310 315 320Ala Ala His Ser Asp Pro Ala His Phe Thr Asp Gly Glu Arg Leu Asp
325 330 335Leu Thr Arg Asp Ser Ala Val His Leu Gln Phe Gly His Gly Ile His
340 345 350Tyr Cys Ile Gly Ala Pro Leu Ala Arg Val Glu Gly Gln Ile Ala Leu
355 360 365Glu Ser Leu Val Arg Arg Phe Pro Gly Leu Arg Leu Ser Val Pro Ala
370 375 380Ala Glu Ile Ser His Ser Lys Asn Pro Phe Ile Arg Ser Leu Thr Ala385 390 395 400Leu Pro Val Glu Phe Glu Ala Gln Gln Pro Val Ala Gly
405 410<210>27<211>678<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1)..(678)<223><400>27atg aac gcc ctg atc ccc cgt cca cgc ctc gag gtg gcc ccc ggc gcc 48Met Asn Ala Leu Ile Pro Arg Pro Arg Leu Glu Val Ala Pro Gly Ala1 5 10 15gtc cat gtg ccg agc tgg ctc acc ctc gaa cag cag cgg gag ctg gtc 96Val His Val Pro Ser Trp Leu Thr Leu Glu Gln Gln Arg Glu Leu Val
20 25 30ctc gcc tgc cgg ggc tgg gcc acc ggc ccg gtc ccg atc cgg cac acc 144Leu Ala Cys Arg Gly Trp Ala Thr Gly Pro Val Pro Ile Arg His Thr
35 40 45aag ctg ccg cgc ggg ggc gtc atg tcg gtg cgc acg gtg tgc atc ggc 192Lys Leu Pro Arg Gly Gly Val Met Ser Val Arg Thr Val Cys Ile Gly
50 55 60tgg cac tgg cag ccc tac gcc tac acc cgc acc gcc gac gat gtg aac 240Trp His Trp Gln Pro Tyr Ala Tyr Thr Arg Thr Ala Asp Asp Val Asn65 70 75 80ggc gcc cgg gtc gcc gaa ttc ccc gac tgg atg gtc gag ttg ggc cgt 288Gly Ala Arg Val Ala Glu Phe Pro Asp Trp Met Val Glu Leu Gly Arg
85 90 95cgc gcc ctg gtc gac gcg tac gac gac gag acg gcc ggt gag ggg tac 336Arg Ala Leu Val Asp Ala Tyr Asp Asp Glu Thr Ala Gly Glu Gly Tyr
100 105 110acc ccc gac acc gcg ctc atc aac ttc tac gac gcc cag gcg aag ctg 384Thr Pro Asp Thr Ala Leu Ile Asn Phe Tyr Asp Ala Gln Ala Lys Leu
115 120 125ggc atg cac cag gac aag gac gag agg tca tcc gcc ccg gtg gtc tcg 432Gly Met His Gln Asp Lys Asp Glu Arg Ser Ser Ala Pro Val Val Ser
130 135 140ctc acc atc ggc gac agc tgt gtc ttc cgc ttc ggc aac acc gag acc 480Leu Thr Ile Gly Asp Ser Cys Val Phe Arg Phe Gly Asn Thr Glu Thr145 150 155 160cgt acc aag ccg tac acc gac ctc gaa ctc gct tcc ggg gat ctg ttc 528Arg Thr Lys Pro Tyr Thr Asp Leu Glu Leu Ala Ser Gly Asp Leu Phe
165 170 175gtc ttc gga ggc ccc tcc cgc tac gcc tat cac gcc gtc ccc agg atc 576Val Phe Gly Gly Pro Ser Arg Tyr Ala Tyr His Ala Val Pro Arg Ile
180 185 190ctg ccc gga acc ggt gac ccg gcc acc gga ctg aag tcc ggg cgg ctg 624Leu Pro Gly Thr Gly Asp Pro Ala Thr Gly Leu Lys Ser Gly Arg Leu
195 200 205aac atc acc atg cgg gtc acc ggt ctg gcc gat ccc cag tcg tca gtc 672Asn Ile Thr Met Arg Val Thr Gly Leu Ala Asp Pro Gln Ser Ser Val
210 215 220gtc ccg 678Val Pro225<210>28<211>226<212>PRT<213>Streptomyces hygroscopicus<400>28Met Asn Ala Leu Ile Pro Arg Pro Arg Leu Glu Val Ala Pro Gly Ala1 5 10 15Val His Val Pro Ser Trp Leu Thr Leu Glu Gln Gln Arg Glu Leu Val
20 25 30Leu Ala Cys Arg Gly Trp Ala Thr Gly Pro Val Pro Ile Arg His Thr
35 40 45Lys Leu Pro Arg Gly Gly Val Met Ser Val Arg Thr Val Cys Ile Gly
50 55 60Trp His Trp Gln Pro Tyr Ala Tyr Thr Arg Thr Ala Asp Asp Val Asn65 70 75 80Gly Ala Arg Val Ala Glu Phe Pro Asp Trp Met Val Glu Leu Gly Arg
85 90 95Arg Ala Leu Val Asp Ala Tyr Asp Asp Glu Thr Ala Gly Glu Gly Tyr
100 105 110Thr Pro Asp Thr Ala Leu Ile Asn Phe Tyr Asp Ala Gln Ala Lys Leu
115 120 125Gly Met His Gln Asp Lys Asp Glu Arg Ser Ser Ala Pro Val Val Ser
130 135 140Leu Thr Ile Gly Asp Ser Cys Val Phe Arg Phe Gly Asn Thr Glu Thr 145 150 155 160Arg Thr Lys Pro Tyr Thr Asp Leu Glu Leu Ala Ser Gly Asp Leu Phe
165 170 175Val Phe Gly Gly Pro Ser Arg Tyr Ala Tyr His Ala Val Pro Arg Ile
180 185 190Leu Pro Gly Thr Gly Asp Pro Ala Thr Gly Leu Lys Ser Gly Arg Leu
195 200 205Asn Ile Thr Met Arg Val Thr Gly Leu Ala Asp Pro Gln Ser Ser Val
210 215 220Val Pro225<210>29<211>726<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1)..(726)<223><400>29atg agc acc acg aac gac acc gca cgc atc cat cag cgc gtg gcc gcg 48Met Ser Thr Thr Asn Asp Thr Ala Arg Ile His Gln Arg Val Ala Ala1 5 10 15gcc gac tgg cca cag ctg gcc gag gag ctg gac acc tac ggg tgc gcg 96Ala Asp Trp Pro Gln Leu Ala Glu Glu Leu Asp Thr Tyr Gly Cys Ala
20 25 30ctc act cca cgg ctg ctg acc ccc gcc cag tgc gcc cgc atc gcc ggg 144Leu Thr Pro Arg Leu Leu Thr Pro Ala Gln Cys Ala Arg Ile Ala Gly
35 40 45ctg tac ggg cag gac gag cag ttc agg aac acg atc gac atg gcc cgc 192Leu Tyr Gly Gln Asp Glu Gln Phe Arg Asn Thr Ile Asp Met Ala Arg
50 55 60cac cgc ttc ggc tcc gga cag tac cgc tac ttc acc cat gac ctg ccc 240His Arg Phe Gly Ser Gly Gln Tyr Arg Tyr Phe Thr His Asp Leu Pro65 70 75 80gaa ccg gtg gcc gag ctg cgc gcc gcg ctc tat ccg cgg ctg ctg acc 288Glu Pro Val Ala Glu Leu Arg Ala Ala Leu Tyr Pro Arg Leu Leu Thr
85 90 95atc gcg cgt gac tgg gcg gag cgg ctc ggc cgc ccg gcg ccc tgg ccg 336Ile Ala Arg Asp Trp Ala Glu Arg Leu Gly Arg Pro Ala Pro Trp Pro
100 105 110gac agc ctc gag aag tgg ctg gcc atg tgt cat gag gcc gga cag gac 384Asp Ser Leu Glu Lys Trp Leu Ala Met Cys His Glu Ala Gly Gln Asp
115 120 125cgc tcc gcg cag atc ctg ctg cgc tac ggc ccc ggc gac tgg aac gcc 432Arg Ser Ala Gln Ile Leu Leu Arg Tyr Gly Pro Gly Asp Trp Asn Ala
130 135 140ctg cac cgg gac gta ttc ggc gac atg ctc ttc ccg ctc cag gtg gtg 480Leu His Arg Asp Val Phe Gly Asp Met Leu Phe Pro Leu Gln Val Val145 150 155 160atc ggg ctc gac gcg tac ggc acg gac tac acg ggc ggg gag ttc ctg 528Ile Gly Leu Asp Ala Tyr Gly Thr Asp Tyr Thr Gly Gly Glu Phe Leu
165 170 175ctg gtc gag cag cgg ccc cgc gcc cag tcc cgg ggc acc acg acc gtc 576Leu Val Glu Gln Arg Pro Arg Ala Gln Ser Arg Gly Thr Thr Thr Val
180 185 190ctc cag cag ggc cac ggc ctg atc ttc acc acc cgt gac cgt ccc gtg 624Leu Gln Gln Gly His Gly Leu Ile Phe Thr Thr Arg Asp Arg Pro Val
195 200 205gcc acc aag cgc ggc tgg tcg gcc ggt gtg atg cgg cac ggg gtc agc 672Ala Thr Lys Arg Gly Trp Ser Ala Gly Val Met Arg His Gly Val Ser
210 215 220acg gtg cgt tcc ggg cgc cgc cac gca ttg ggg ctg gtc ttc cac gac 720Thr Val Arg Ser Gly Arg Arg His Ala Leu Gly Leu Val Phe His Asp225 230 235 240gcc gcc 726Ala Ala<210>30<211>242<212>PRT<213>Streptomyces hygroscopicus<400>30Met Ser Thr Thr Asn Asp Thr Ala Arg Ile His Gln Arg Val Ala Ala1 5 10 15Ala Asp Trp Pro Gln Leu Ala Glu Glu Leu Asp Thr Tyr Gly Cys Ala
20 25 30Leu Thr Pro Arg Leu Leu Thr Pro Ala Gln Cys Ala Arg Ile Ala Gly
35 40 45Leu Tyr Gly Gln Asp Glu Gln Phe Arg Asn Thr Ile Asp Met Ala Arg
50 55 60His Arg Phe Gly Ser Gly Gln Tyr Arg Tyr Phe Thr His Asp Leu Pro65 70 75 80Glu Pro Val Ala Glu Leu Arg Ala Ala Leu Tyr Pro Arg Leu Leu Thr
85 90 95Ile Ala Arg Asp Trp Ala Glu Arg Leu Gly Arg Pro Ala Pro Trp Pro
100 105 110Asp Ser Leu Glu Lys Trp Leu Ala Met Cys His Glu Ala Gly Gln Asp
115 120 125Arg Ser Ala Gln Ile Leu Leu Arg Tyr Gly Pro Gly Asp Trp Asn Ala
130 135 140Leu His Arg Asp Val Phe Gly Asp Met Leu Phe Pro Leu Gln Val Val145 150 155 160Ile Gly Leu Asp Ala Tyr Gly Thr Asp Tyr Thr Gly Gly Glu Phe Leu
165 170 175Leu Val Glu Gln Arg Pro Arg Ala Gln Ser Arg Gly Thr Thr Thr Val
180 185 190Leu Gln Gln Gly His Gly Leu Ile Phe Thr Thr Arg Asp Arg Pro Val
195 200 205Ala Thr Lys Arg Gly Trp Ser Ala Gly Val Met Arg His Gly Val Ser
210 215 220Thr Val Arg Ser Gly Arg Arg His Ala Leu Gly Leu Val Phe His Asp225 230 235 240Ala Ala<210>31<211>504<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1).. (504)<223><400>31atg acg gtc cac acg acg atc gac agc ccg ctc ggc gag ctg ctg ctg 48Met Thr Val His Thr Thr Ile Asp Ser Pro Leu Gly Glu Leu Leu Leu1 5 10 15gtg ggc gag gag tcc gcc acc gcg ccg ggg ggc acc gca ctc atc tcc 96Val Gly Glu Glu Ser Ala Thr Ala Pro Gly Gly Thr Ala Leu Ile Ser
20 25 30ctg tcc gtg ccc ggc cag aag ggc ggg gcc gtc gtc cag gac ggt tgg 144Leu Ser Val Pro Gly Gln Lys Gly Gly Ala Val Val Gln Asp Gly Trp
35 40 45agc gag gat gcc gag gcg ttc acc gag atc gtc tcc cag ttg cgc tcc 192Ser Glu Asp Ala Glu Ala Phe Thr Glu Ile Val Ser Gln Leu Arg Ser
50 55 60tac ttc gac ggc gag cgc acc cgc ttc gac atc gag tgc gtc gag ggc 240Tyr Phe Asp Gly Glu Arg Thr Arg Phe Asp Ile Glu Cys Val Glu Gly65 70 75 80ggt acg gac ttc cag cgc agg gtc tgg cag gcg ctg gag gcc att ccg 288Gly Thr Asp Phe Gln Arg Arg Val Trp Gln Ala Leu Glu Ala Ile Pro
85 90 95tac ggc acc act gtc agc tac ggc gac atc gcc cgg cag atc ggc gcc 336Tyr Gly Thr Thr Val Ser Tyr Gly Asp Ile Ala Arg Gln Ile Gly Ala
100 105 110ccg cgc acg gcc gtc cgc tcc gtc ggc acc gcg arc ggc cgc aat cca 384Pro Arg Thr Ala Val Arg Ser Val Gly Thr Ala Ile Gly Arg Asn Pro
115 120 125ctg ctg gtc gtg cgg ccc tgc cac cgg gtc atc ggc gcc acc ggc gca 432Leu Leu Val Val Arg Pro Cys His Arg Val Ile Gly Ala Thr Gly Ala
130 135 140ctg acc ggc tat gcg ggc gga ctg gag cgc aag cag cga ctc ctc gtt 480Leu Thr Gly Tyr Ala Gly Gly Leu Glu Arg Lys Gln Arg Leu Leu Val 145 150 155 160cac gag ggc gcc ctc cag acc gcc 504His Glu Gly Ala Leu Gln Thr Ala
165<210>32<211>168<212>PRT<213>Streptomyces hygroscopicus<400>32Met Thr Val His Thr Thr Ile Asp Ser Pro Leu Gly Glu Leu Leu Leu1 5 10 15Val Gly Glu Glu Ser Ala Thr Ala Pro Gly Gly Thr Ala Leu Ile Ser
20 25 30Leu Ser Val Pro Gly Gln Lys Gly Gly Ala Val Val Gln Asp Gly Trp
35 40 45Ser Glu Asp Ala Glu Ala Phe Thr Glu Ile Val Ser Gln Leu Arg Ser
50 55 60Tyr Phe Asp Gly Glu Arg Thr Arg Phe Asp Ile Glu Cys Val Glu Gly65 70 75 80Gly Thr Asp Phe Gln Arg Arg Val Trp Gln Ala Leu Glu Ala Ile Pro
85 90 95Tyr Gly Thr Thr Val Ser Tyr Gly Asp Ile Ala Arg Gln Ile Gly Ala
100 105 110Pro Arg Thr Ala Val Arg Ser Val Gly Thr Ala Ile Gly Arg Asn Pro
115 120 125Leu Leu Val Val Arg Pro Cys His Arg Val Ile Gly Ala Thr Gly Ala
130 135 140Leu Thr Gly Tyr Ala Gly Gly Leu Glu Arg Lys Gln Arg Leu Leu Val145 150 155 160His Glu Gly Ala Leu Gln Thr Ala
165<210>33<211>618<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1)..(618)<223><400>33atg cac gag gga cac ggc cac cag gag tac atc gtc acg tcc gac ccc 48Met His Glu Gly His Gly His Gln Glu Tyr Ile Val Thr Ser Asp Pro1 5 10 15gag gcg gtg gcc cgt gtc cgg gcc tcc ttg ctg cgc acc ctg cca gtg 96Glu Ala Val Ala Arg Val Arg Ala Ser Leu Leu Arg Thr Leu Pro Val
20 25 30gcc gca tgg gcc ggg gtg gcc agt gcc gtg gtc atc gtc tcg gcc gtg 144Ala Ala Trp Ala Gly Val Ala Ser Ala Val Val Ile Val Ser Ala Val
35 40 45gcc ctc gtc ctc ttc gcc ctc ggc cac agc gcc tcc cgg ctg tgg gtc 192Ala Leu Val Leu Phe Ala Leu Gly His Ser Ala Ser Arg Leu Trp Val
50 55 60ctg gtg ttc gcc tgg ccc gcg gcg ttc ctc ggc tat gac gcc agg cgc 240Leu Val Phe Ala Trp Pro Ala Ala Phe Leu Gly Tyr Asp Ala Arg Arg65 70 75 80cga ttc gcc gat ata cgg cgg ctg aag cgg acc tgg gcg gcg aaa gag 288Arg Phe Ala Asp Ile Arg Arg Leu Lys Arg Thr Trp Ala Ala Lys Glu
85 90 95gtg tcc ccg gtg gcg atg cgc ctc tcc gcc gag ggc ctg cgc tgc gcc 336Val Ser Pro Val Ala Met Arg Leu Ser Ala Glu Gly Leu Arg Cys Ala
100 105 110atc gac tcc gcc ccg gag ccc gtt ttc ctc ccc tgg tcc gcg atc gcc 384Ile Asp Ser Ala Pro Glu Pro Val Phe Leu Pro Trp Ser Ala Ile Ala
115 120 125cag gtg cgg gtg acg ggc cag ggc ctc agc acg gtg cgg gtc gat ctc 432Gln Val Arg Val Thr Gly Gln Gly Leu Ser Thr Val Arg Val Asp Leu
130 135 140gcc ccc ggc gtg tcc gcc acc acc ccc ggg gtc agc ggg ctg gag cag 480Ala Pro Gly Val Ser Ala Thr Thr Pro Gly Val Ser Gly Leu Glu Gln145 150 155 160ccc gag gcc cgg atg cgc atg cgg cgc gcc tgg aac ggc ggg atg cgg 528Pro Glu Ala Arg Met Arg Met Arg Arg Ala Trp Asn Gly Gly Met Arg
165 170 175ctg cgc ttc acc gtc tac gcc ctc cgc cag ccg atc agc gag atc gac 576Leu Arg Phe Thr Val Tyr Ala Leu Arg Gln Pro Ile Ser Glu Ile Asp
180 185 190cag gct ctc ggc cac ttc tcg aac ggg cgg atc ggt atc cgc 618Gln Ala Leu Gly His Phe Ser Asn Gly Arg Ile Gly Ile Arg
195 200 205<210>34<211>206<212>PRT<213>Streptomyces hygroscopicus<400>34Met His Glu Gly His Gly His Gln Glu Tyr Ile Val Thr Ser Asp Pro1 5 10 15Glu Ala Val Ala Arg Val Arg Ala Ser Leu Leu Arg Thr Leu Pro Val
20 25 30Ala Ala Trp Ala Gly Val Ala Ser Ala Val Val Ile Val Ser Ala Val
35 40 45Ala Leu Val Leu Phe Ala Leu Gly His Ser Ala Ser Arg Leu Trp Val
50 55 60Leu Val Phe Ala Trp Pro Ala Ala Phe Leu Gly Tyr Asp Ala Arg Arg65 70 75 80Arg Phe Ala Asp Ile Arg Arg Leu Lys Arg Thr Trp Ala Ala Lys Glu
85 90 95Val Ser Pro Val Ala Met Arg Leu Ser Ala Glu Gly Leu Arg Cys Ala
100 105 110Ile Asp Ser Ala Pro Glu Pro Val Phe Leu Pro Trp Ser Ala Ile Ala
115 120 125Gln Val Arg Val Thr Gly Gln Gly Leu Ser Thr Val Arg Val Asp Leu
130 135 140Ala Pro Gly Val Ser Ala Thr Thr Pro Gly Val Ser Gly Leu Glu Gln145 150 155 160Pro Glu Ala Arg Met Arg Met Arg Arg Ala Trp Asn Gly Gly Met Arg
165 170 175Leu Arg Phe Thr Val Tyr Ala Leu Arg Gln Pro Ile Ser Glu Ile Asp
180 185 190Gln Ala Leu Gly His Phe Ser Asn Gly Arg Ile Gly Ile Arg
195 200 205<210>35<211>2721<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1)..(2721)<223><400>35atg gga gga cgt gct cgt ccg gct cgg cga cgg ctg ggg ccc ctg tcg 48Met Gly Gly Arg Ala Arg Pro Ala Arg Arg Arg Leu Gly Pro Leu Ser 1 5 10 15tat acg cga agc gtc gcc ggg cag gcg ttc atc ttg cag ctt ctg ctg 96Tyr Thr Arg Ser Val Ala Gly Gln Ala Phe Ile Leu Gln Leu Leu Leu
20 25 30atc ctg gtt ctg gtg gcc gcg gcg gtg gtg gcc gtc gca gcg gat gcc 144Ile Leu Val Leu Val Ala Ala Ala Val Val Ala Val Ala Ala Asp Ala
35 40 45cgg agc cac agc acg acc gac gct cgc cgg cga tcc ctc gcg gtc gcc 192Arg Ser His Ser Thr Thr Asp Ala Arg Arg Arg Ser Leu Ala Val Ala
50 55 60gag acc ttg gca cac tcc ccc gga atg gcc cgg gcc ctg acc agc gac 240Glu Thr Leu Ala His Ser Pro Gly Met Ala Arg Ala Leu Thr Ser Asp65 70 75 80cgg ccg acg tcg ctg ctg gag tcg cat gcg gag gcg gcg cgg aag aga 288Arg Pro Thr Ser Leu Leu Glu Ser His Ala Glu Ala Ala Arg Lys Arg
85 90 95rca ggc gtc gac agc gtc gtg gtg ttc aac act cat ggc atc cgc ctc 336Ser Gly Val Asp Ser Val Val Val Phe Asn Thr His Gly Ile Arg Leu
100 105 110acc cac ccc gag aag gca ttg arc ggc aag cgg arc gtc gga ccg gcc 384Thr His Pro Glu Lys Ala Leu Ile Gly Lys Arg Ile Val Gly Pro Ala
115 120 125ggg ctg gtg cgg gac gag ctg aaa ggc aag acg atc acg gag tcc ttc 432Gly Leu Val Arg Asp Glu Leu Lys Gly Lys Thr Ile Thr Glu Ser Phe
130 135 140cag gcc agc cag ggc ccg tcc gtg gtc tcg gcg gtc ccc gtc acc agg 480Gln Ala Ser Gln Gly Pro Ser Val Val Ser Ala Val Pro Val Thr Arg145 150 155 160gcc gac ggc acc ttc ctc ggc ggt gtg tcc gtc ggg gtc aag atc gcg 528Ala Asp Gly Thr Phe Leu Gly Gly Val Ser Val Gly Val Lys Ile Ala
165 170 175agc gtg aac agc gag gtg gac cgt cgg cta ccg ctg ctg ctc ggc agt 576Ser Val Asn Ser Glu Val Asp Arg Arg Leu Pro Leu Leu Leu Gly Ser
180 185 190ggc acc ggg gca ctg gcc ctg gcc tcg ggc ggg gcg gcg ctg atg agc 624Gly Thr Gly Ala Leu Ala Leu Ala Ser Gly Gly Ala Ala Leu Met Ser
195 200 205agg cgg gtg cgg cgg cag acc cac ggc ctg ggc gcc gcg gag atg acg 672Arg Arg Val Arg Arg Gln Thr His Gly Leu Gly Ala Ala Glu Met Thr
210 215 220cgg atg tac gag cac cat gac gcg gtg ttg cgc tcg gtc cgc gaa ggg 720Arg Met Tyr Glu His His Asp Ala Val Leu Arg Ser Val Arg Glu Gly225 230 235 240gtg ctg gtc ctg acc gcg ggc ggg cgg ctg ctg gtg gtc aac gac gag 768Val Leu Val Leu Thr Ala Gly Gly Arg Leu Leu Val Val Asn Asp Glu
245 250 255gcc cgg gaa ctg ctc ggg ctg gct ccg gac gcg gag ggg cgg cgc atc 816Ala Arg Glu Leu Leu Gly Leu Ala Pro Asp Ala Glu Gly Arg Arg Ile
260 265 270gac gag ctc ggc ctc gaa ccg cac ctg acg caa ctg ctg gcg tcg gga 864Asp Glu Leu Gly Leu Glu Pro His Leu Thr Gln Leu Leu Ala Ser Gly
275 280 285cgg cgc gtc acc gac gag gtg cac ccc cgc ggg gat cga cta ctg gcg 912Arg Arg Val Thr Asp Glu Val His Pro Arg Gly Asp Arg Leu Leu Ala
290 295 300gtc aat atg cgg tcc acg gac cgt gcg ggc gat ccc gcc gga aac gtg 960Val Asn Met Arg Ser Thr Asp Arg Ala Gly Asp Pro Ala Gly Asn Val305 310 315 320gtg acg ctg agg gac acc acc gcg ctg cgg gtg ctg tcc gac cgg gcc 1008Val Thr Leu Arg Asp Thr Thr Ala Leu Arg Val Leu Ser Asp Arg Ala
325 330 335gag caa gcc ggt gag cgg ctg aag ctg ctg tcc gac gcc ggg gtg cgg 1056Glu Gln Ala Gly Glu Arg Leu Lys Leu Leu Ser Asp Ala Gly Val Arg
340 345 350atc agc tcc agc ctg gag ctg acg ggc acc gcg gag aag ctg gtg gac 1104Ile Ser Ser Ser Leu Glu Leu Thr Gly Thr Ala Glu Lys Leu Val Asp
355 360 365gtg gcc gtc ccc cgg ttc gcc gac atc gtc tcg gtc gaa ctg ctg gag 1152Val Ala Val Pro Arg Phe Ala Asp Ile Val Ser Val Glu Leu Leu Glu
370 375 380ccc gtg ctg cgc ggc gag gag ccc gag ccg ccg tac gag cca ctg gcg 1200Pro Val Leu Arg Gly Glu Glu Pro Glu Pro Pro Tyr Glu Pro Leu Ala385 390 395 400ccg cac cgg acc gcc gtc ggc gga gat ccc ccc gac ggc ctc gtc ttc 1248Pro His Arg Thr Ala Val Gly Gly Asp Pro Pro Asp Gly Leu Val Phe
405 410 415cgc gtg ggc gag cga gtc gtc tac gca ccc tcc aca ccg cag agc cgg 1296Arg Val Gly Glu Arg Val Val Tyr Ala Pro Ser Thr Pro Gln Ser Arg
420 425 430gcc gtg aag gcc gga gcc gcc gtc ctc ctg acc gat ctg acg ggc ccc 1344Ala Val Lys Ala Gly Ala Ala Val Leu Leu Thr Asp Leu Thr Gly Pro
435 440 445ggc gag tcg ccg agc gac cac tcc gcc ccg tac cag tcc ccc ggg caa 1392Gly Glu Ser Pro Ser Asp His Ser Ala Pro Tyr Gln Ser Pro Gly Gln
450 455 460tcg gcc acg tac agt gcc gag acc cgg cgc ctc ctc gac cgc ggg gtc 1440Ser Ala Thr Tyr Ser Ala Glu Thr Arg Arg Leu Leu Asp Arg Gly Val465 470 475 480cac tcg ctg atc acc gtc ccg ctg cgg ttc cgc ggg gtc acc ctc ggc 1488His Ser Leu Ile Thr Val Pro Leu Arg Phe Arg Gly Val Thr Leu Gly
485 490 495ctg gcc acc ttc tgg cgg acc cgg ccc ggt gag ccg ttc gac gag gcg 1536Leu Ala Thr Phe Trp Arg Thr Arg Pro Gly Glu Pro Phe Asp Glu Ala
500 505 510gat ctg gcg atc gcc ggg gag ctg gcc gtg cgc acc gcc gta tgt gtc 1584Asp Leu Ala Ile Ala Gly Glu Leu Ala Val Arg Thr Ala Val Cys Val
515 520 525gac aac gcc cgc cgc tac gcc cgc gaa cac acc atg gtc acc acc ttg 1632Asp Asn Ala Arg Arg Tyr Ala Arg Glu His Thr Met Val Thr Thr Leu
530 535 540cag cgc acc ctc ctc ccc agc ggt ctg ccc gat cag gac gcc gtg cgg 1680Gln Arg Thr Leu Leu Pro Ser Gly Leu Pro Asp Gln Asp Ala Val Arg545 550 555 560gtg gcg tcc cgc tat ctg ccc gca cag ggc gag acg ggc gga tcc tgg 1728Val Ala Ser Arg Tyr Leu Pro Ala Gln Gly Glu Thr Gly Gly Ser Trp
565 570 575ttc gat gtg atc cct ctc ccc ggg gcc cgg gtc gcg ctg gtc gtc ggg 1776Phe Asp Val Ile Pro Leu Pro Gly Ala Arg Val Ala Leu Val Val Gly
580 585 590aag gtg gcc ggg cag ggc ctg cac gcc gcg gcc acg atg ggg cgg ctg 1824Lys Val Ala Gly Gln Gly Leu His Ala Ala Ala Thr Met Gly Arg Leu
595 600 605cgc acc gcg gtg cag aac ttc tcg gcc ctg gac gtg ccc ccg gat gag 1872Arg Thr Ala Val Gln Asn Phe Ser Ala Leu Asp Val Pro Pro Asp Glu
610 615 620ctc ctc tcc cat ctg gac gag ctg gtc acc cgt ctc gac ctg gag cgc 1920Leu Leu Ser His Leu Asp Glu Leu Val Thr Arg Leu Asp Leu Glu Arg625 630 635 640gag gcc gat tcg gac gac gtc cgg atc acg ggc gcc acc tgc ctg tac 1968Glu Ala Asp Ser Asp Asp Val Arg Ile Thr Gly Ala Thr Cys Leu Tyr
645 650 655gcg atc cac gac tcg gtg tcc ggc cac tgc gcc atg gcc cgg gcc ggc 2016Ala Ile His Asp Ser Val Ser Gly His Cys Ala Met Ala Arg Ala Gly
660 665 670gat ccg ggc atc gcc gtg acc cac ccg gac ggc acc gtg gac ctc cct 2064Asp Pro Gly Ile Ala Val Thr His Pro Asp Gly Thr Val Asp Leu Pro
675 680 685gcg gta ccc atc ggc ccg gcc ctg ggc atg ggc ggg gag ccg ttc gag 2112Ala Val Pro Ile Gly Pro Ala Leu Gly Met Gly Gly Glu Pro Phe Glu
690 695 700gcg gtc ggc ctc tcg ctg ccc gcc gca agc cgg ctg gtg ctg tac acc 2160Ala Val Gly Leu Ser Leu Pro Ala Ala Ser Arg Leu Val Leu Tyr Thr 705 710 715 720aac ggc ctt ctt gaa ggg gaa ggc caa gcc gcc gac acc ggc ctc gac 2208Asn Gly Leu Leu Glu Gly Glu Gly Gln Ala Ala Asp Thr Gly Leu Asp
725 730 735ctg ctg cgc cgc acc ctc gcg gcc gag ccg gac ctc ggc ccg gac gag 2256Leu Leu Arg Arg Thr Leu Ala Ala Glu Pro Asp Leu Gly Pro Asp Glu
740 745 750acc tgc cgg agc ctt ttc gac acc gtg ctt ccg gcc cac ccg agc gac 2304Thr Cys Arg Ser Leu Phe Asp Thr Val Leu Pro Ala His Pro Ser Asp
755 760 765gat gtg gcg ctg ctg gtg gcc cgg acc cgc ctg ctc gcc ccg gag aac 2352Asp Val Ala Leu Leu Val Ala Arg Thr Arg Leu Leu Ala Pro Glu Asn
770 775 780gtg gcc gag tgg gat gtg ccg ttc gac ctg gcg gcg gtc gcc ccg ctg 2400Val Ala Glu Trp Asp Val Pro Phe Asp Leu Ala Ala Val Ala Pro Leu785 790 795 800cgc gcc acc tgc acc cgg aaa ctg cgg gcg tgg ggc ctg gag gac gcc 2448Arg Ala Thr Cys Thr Arg Lys Leu Arg Ala Trp Gly Leu Glu Asp Ala
805 810 815gcg tac acc gcc gag ctg atc atc agt gaa ctg atc acc aac gcc ctg 2496Ala Tyr Thr Ala Glu Leu Ile Ile Ser Glu Leu Ile Thr Asn Ala Leu
820 825 830cgg tac ggc tcc cct ccc gta cgc ata cgg ctg ctg cgc ggc cgc ggc 2544Arg Tyr Gly Ser Pro Pro Val Arg Ile Arg Leu Leu Arg Gly Arg Gly
835 840 845ctg atc ttc gag gtc tcc gac ggc agc agc acc gca ccc cat ctg cgg 2592Leu Ile Phe Glu Val Ser Asp Gly Ser Ser Thr Ala Pro His Leu Arg
850 855 860cgg gcc gcg atc acc gac gag ggc ggc cgc ggg ctg ttc ctc gtc gcc 2640Arg Ala Ala Ile Thr Asp Glu Gly Gly Arg Gly Leu Phe Leu Val Ala865 870 875 880cag ttc gcc cag cgc tgg ggc acc cgc tac acc ccg cac ggc aag gtc 2688Gln Phe Ala Gln Arg Trp Gly Thr Arg Tyr Thr Pro His Gly Lys Val
885 890 895atc tgg gcc gag gcg gcc ctg gac ggc ggc ctc 2721Ile Trp Ala Glu Ala Ala Leu Asp Gly Gly Leu
900 905<210>36<211>907<212>PRT<213>Streptomyces hygroscopicus<400>36Met Gly Gly Arg Ala Arg Pro Ala Arg Arg Arg Leu Gly Pro Leu Ser1 5 10 15Tyr Thr Arg Ser Val Ala Gly Gln Ala Phe Ile Leu Gln Leu Leu Leu
20 25 30Ile Leu Val Leu Val Ala Ala Ala Val Val Ala Val Ala Ala Asp Ala
35 40 45Arg Ser His Ser Thr Thr Asp Ala Arg Arg Arg Ser Leu Ala Val Ala
50 55 60Glu Thr Leu Ala His Ser Pro Gly Met Ala Arg Ala Leu Thr Ser Asp65 70 75 80Arg Pro Thr Ser Leu Leu Glu Ser His Ala Glu Ala Ala Arg Lys Arg
85 90 95Ser Gly Val Asp Ser Val Val Val Phe Asn Thr His Gly Ile Arg Leu
100 105 110Thr His Pro Glu Lys Ala Leu Ile Gly Lys Arg Ile Val Gly Pro Ala
115 120 125Gly Leu Val Arg Asp Glu Leu Lys Gly Lys Thr Ile Thr Glu Ser Phe
130 135 140Gln Ala Ser Gln Gly Pro Ser Val Val Ser Ala Val Pro Val Thr Arg145 150 155 160Ala Asp Gly Thr Phe Leu Gly Gly Val Ser Val Gly Val Lys Ile Ala
165 170 175Ser Val Asn Ser Glu Val Asp Arg Arg Leu Pro Leu Leu Leu Gly Ser
180 185 190Gly Thr Gly Ala Leu Ala Leu Ala Ser Gly Gly Ala Ala Leu Met Ser
195 200 205Arg Arg Val Arg Arg Gln Thr His Gly Leu Gly Ala Ala Glu Met Thr
210 215 220Arg Met Tyr Glu His His Asp Ala Val Leu Arg Ser Val Arg Glu Gly225 230 235 240Val Leu Val Leu Thr Ala Gly Gly Arg Leu Leu Val Val Asn Asp Glu
245 250 255Ala Arg Glu Leu Leu Gly Leu Ala Pro Asp Ala Glu Gly Arg Arg Ile
260 265 270Asp Glu Leu Gly Leu Glu Pro His Leu Thr Gln Leu Leu Ala Ser Gly
275 280 285Arg Arg Val Thr Asp Glu Val His Pro Arg Gly Asp Arg Leu Leu Ala
290 295 300Val Asn Met Arg Ser Thr Asp Arg Ala Gly Asp Pro Ala Gly Asn Val305 310 315 320Val Thr Leu Arg Asp Thr Thr Ala Leu Arg Val Leu Ser Asp Arg Ala
325 330 335Glu Gln Ala Gly Glu Arg Leu Lys Leu Leu Ser Asp Ala Gly Val Arg
340 345 350Ile Ser Ser Ser Leu Glu Leu Thr Gly Thr Ala Glu Lys Leu Val Asp
355 360 365Val Ala Val Pro Arg Phe Ala Asp Ile Val Ser Val Glu Leu Leu Glu
370 375 380Pro Val Leu Arg Gly Glu Glu Pro Glu Pro Pro Tyr Glu Pro Leu Ala385 390 395 400Pro His Arg Thr Ala Val Gly Gly Asp Pro Pro Asp Gly Leu Val Phe
405 410 415Arg Val Gly Glu Arg Val Val Tyr Ala Pro Ser Thr Pro Gln Ser Arg
420 425 430Ala Val Lys Ala Gly Ala Ala Val Leu Leu Thr Asp Leu Thr Gly Pro
435 440 445Gly Glu Ser Pro Ser Asp His Ser Ala Pro Tyr Gln Ser Pro Gly Gln
450 455 460Ser Ala Thr Tyr Ser Ala Glu Thr Arg Arg Leu Leu Asp Arg Gly Val465 470 475 480His Ser Leu Ile Thr Val Pro Leu Arg Phe Arg Gly Val Thr Leu Gly
485 490 495Leu Ala Thr Phe Trp Arg Thr Arg Pro Gly Glu Pro Phe Asp Glu Ala
500 505 510Asp Leu Ala Ile Ala Gly Glu Leu Ala Val Arg Thr Ala Val Cys Val
515 520 525Asp Asn Ala Arg Arg Tyr Ala Arg Glu His Thr Met Val Thr Thr Leu
530 535 540Gln Arg Thr Leu Leu Pro Ser Gly Leu Pro Asp Gln Asp Ala Val Arg545 550 555 560Val Ala Ser Arg Tyr Leu Pro Ala Gln Gly Glu Thr Gly Gly Ser Trp
565 570 575Phe Asp Val Ile Pro Leu Pro Gly Ala Arg Val Ala Leu Val Val Gly
580 585 590Lys Val Ala Gly Gln Gly Leu His Ala Ala Ala Thr Met Gly Arg Leu
595 600 605Arg Thr Ala Val Gln Asn Phe Ser Ala Leu Asp Val Pro Pro Asp Glu
610 615 620Leu Leu Ser His Leu Asp Glu Leu Val Thr Arg Leu Asp Leu Glu Arg625 630 635 640Glu Ala Asp Ser Asp Asp Val Arg Ile Thr Gly Ala Thr Cys Leu Tyr
645 650 655Ala Ile His Asp Ser Val Ser Gly His Cys Ala Met Ala Arg Ala Gly
660 665 670Asp Pro Gly Ile Ala Val Thr His Pro Asp Gly Thr Val Asp Leu Pro
675 680 685Ala Val Pro Ile Gly Pro Ala Leu Gly Met Gly Gly Glu Pro Phe Glu
690 695 700Ala Val Gly Leu Ser Leu Pro Ala Ala Ser Arg Leu Val Leu Tyr Thr705 710 715 720Asn Gly Leu Leu Glu Gly Glu Gly Gln Ala Ala Asp Thr Gly Leu Asp
725 730 735Leu Leu Arg Arg Thr Leu Ala Ala Glu Pro Asp Leu Gly Pro Asp Glu
740 745 750Thr Cys Arg Ser Leu Phe Asp Thr Val Leu Pro Ala His Pro Ser Asp
755 760 765Asp Val Ala Leu Leu Val Ala Arg Thr Arg Leu Leu Ala Pro Glu Asn
770 775 780Val Ala Glu Trp Asp Val Pro Phe Asp Leu Ala Ala Val Ala Pro Leu785 790 795 800Arg Ala Thr Cys Thr Arg Lys Leu Arg Ala Trp Gly Leu Glu Asp Ala
805 810 815Ala Tyr Thr Ala Glu Leu Ile Ile Ser Glu Leu Ile Thr Asn Ala Leu
820 825 830Arg Tyr Gly Ser Pro Pro Val Arg Ile Arg Leu Leu Arg Gly Arg Gly
835 840 845Leu Ile Phe Glu Val Ser Asp Gly Ser Ser Thr Ala Pro His Leu Arg
850 855 860Arg Ala Ala Ile Thr Asp Glu Gly Gly Arg Gly Leu Phe Leu Val Ala865 870 875 880Gln Phe Ala Gln Arg Trp Gly Thr Arg Tyr Thr Pro His Gly Lys Val
885 890 895Ile Trp Ala Glu Ala Ala Leu Asp Gly Gly Leu
900 905<210>37<211>969<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1).. (969)<223><400>37gtg aga aca gct cgc cgt acc agg aga cgt ggc cgg ttg acc gcc gcg 48Val Arg Thr Ala Arg Arg Thr Arg Arg Arg Gly Arg Leu Thr Ala Ala1 5 10 15gtg tcc ggt ctg ttc atc acg gca gcc ctc gcg aca gtc ggt acg agt 96Val Ser Gly Leu Phe Ile Thr Ala Ala Leu Ala Thr Val Gly Thr Ser
20 25 30gcg gcc gct tcc tcc gcg atg acc gcc acg tcc gcg ccc agt gcc acc 144Ala Ala Ala Ser Ser Ala Met Thr Ala Thr Ser Ala Pro Ser Ala Thr
35 40 45gcc acg ccc ccg tcc gta tcc acg ccc gtg tcc ggc gcc gcc acg ccc 192Ala Thr Pro Pro Ser Val Ser Thr Pro Val Ser Gly Ala Ala Thr Pro
50 55 60gtg tcc ggc gcc gcc tcg tcc gtg tcc ggc gcc gcc tcg gct gtg gca 240Val Ser Gly Ala Ala Ser Ser Val Ser Gly Ala Ala Ser Ala Val Ala65 70 75 80tcc ctg gat gtc ccg ggc acc gcc tgg acc gtg gac gag cgc acc gga 288Ser Leu Asp Val Pro Gly Thr Ala Trp Thr Val Asp Glu Arg Thr Gly
85 90 95acg ctg cga gtc ctc gtc ggt tcc acg gcc cgg gaa gcc gat ctg gcc 336Thr Leu Arg Val Leu Val Gly Ser Thr Ala Arg Glu Ala Asp Leu Ala
100 105 110agg ctc gac cgc acc gcc gag cgc ttc ggc ggc acg atc acc gtg gag 384Arg Leu Asp Arg Thr Ala Glu Arg Phe Gly Gly Thr Ile Thr Val Glu
115 120 125cgg ctc gac ggt ccg ctg cgg acc ctg ctc tcc ggt ggc gac ggg atc 432Arg Leu Asp Gly Pro Leu Arg Thr Leu Leu Ser Gly Gly Asp Gly Ile
130 135 140cac tcc acc acg ggg ctg cgc tgc tcc gcg ggg gtc aat gtg caa agc 480His Ser Thr Thr Gly Leu Arg Cys Ser Ala Gly Val Asn Val Gln Ser145 150 155 160ggc acc acg tat tac ttc gtc acg gcc ggc cac tgc acc gac gcc gcc 528Gly Thr Thr Tyr Tyr Phe Val Thr Ala Gly His Cys Thr Asp Ala Ala
165 170 175ccc acc tgg tac acc ggc tcc gat gcg acc acc ccg gtc ggt tcg acg 576Pro Thr Trp Tyr Thr Gly Ser Asp Ala Thr Thr Pro Val Gly Ser Thr
180 185 190acc gcc acc agc ttc ccg ggc aat gac tac ggc gtc gtc cgg tac acc 624Thr Ala Thr Ser Phe Pro Gly Asn Asp Tyr Gly Val Val Arg Tyr Thr
195 200 205aac acg gcc gtt ccg cac ccc ggg acc gtg gga acc gtg gac atc acc 672Asn Thr Ala Val Pro His Pro Gly Thr Val Gly Thr Val Asp Ile Thr
210 215 220ggg acc gcc acc gcc tac gtc ggc cag cag gtc tgc cgc cgg ggt gcc 720Gly Thr Ala Thr Ala Tyr Val Gly Gln Gln Val Cys Arg Arg Gly Ala225 230 235 240acg acc ggc gtc cgg tgc ggt cag gtc atc gcg ctc aac gcc acc gtc 768Thr Thr Gly Val Arg Cys Gly Gln Val Ile Ala Leu Asn Ala Thr Val
245 250 255aac tac ggc ggc ggt gat gtc gtc tcc ggc ctg atc cag acc aat atc 816Asn Tyr Gly Gly Gly Asp Val Val Ser Gly Leu Ile Gln Thr Asn Ile
260 265 270tgc gcc gag ccg ggc gac agc ggc ggt ccg ctc tac gcg ggc gac aag 864Cys Ala Glu Pro Gly Asp Ser Gly Gly Pro Leu Tyr Ala Gly Asp Lys
275 280 285atc atc ggc att ctc tcg ggc ggc tcc ggg gac tgc gcg acc gga ggc 912Ile Ile Gly Ile Leu Ser Gly Gly Ser Gly Asp Cys Ala Thr Gly Gly
290 295 300acc acc ttc tac cag ccg atc cag gag gtg ctg agc gcc tac ggc ctc 960Thr Thr Phe Tyr Gln Pro Ile Gln Glu Val Leu Ser Ala Tyr Gly Leu305 310 315 320acc gtc tac 969Thr Val Tyr<210>38<211>323<212>PRT<213>Streptomyces hygroscopicus<400>38Val Arg Thr Ala Arg Arg Thr Arg Arg Arg Gly Arg Leu Thr Ala Ala1 5 10 15Val Ser Gly Leu Phe Ile Thr Ala Ala Leu Ala Thr Val Gly Thr Ser
20 25 30Ala Ala Ala Ser Ser Ala Met Thr Ala Thr Ser Ala Pro Ser Ala Thr
35 40 45Ala Thr Pro Pro Ser Val Ser Thr Pro Val Ser Gly Ala Ala Thr Pro
50 55 60Val Ser Gly Ala Ala Ser Ser Val Ser Gly Ala Ala Ser Ala Val Ala65 70 75 80Ser Leu Asp Val Pro Gly Thr Ala Trp Thr Val Asp Glu Arg Thr Gly
85 90 95Thr Leu Arg Val Leu Val Gly Ser Thr Ala Arg Glu Ala Asp Leu Ala
100 105 110Arg Leu Asp Arg Thr Ala Glu Arg Phe Gly Gly Thr Ile Thr Val Glu
115 120 125Arg Leu Asp Gly Pro Leu Arg Thr Leu Leu Ser Gly Gly Asp Gly Ile
130 135 140His Ser Thr Thr Gly Leu Arg Cys Ser Ala Gly Val Asn Val Gln Ser145 150 155 160Gly Thr Thr Tyr Tyr Phe Val Thr Ala Gly His Cys Thr Asp Ala Ala
165 170 175Pro Thr Trp Tyr Thr Gly Ser Asp Ala Thr Thr Pro Val Gly Ser Thr
180 185 190Thr Ala Thr Ser Phe Pro Gly Asn Asp Tyr Gly Val Val Arg Tyr Thr
195 200 205Asn Thr Ala Val Pro His Pro Gly Thr Val Gly Thr Val Asp Ile Thr
210 215 220Gly Thr Ala Thr Ala Tyr Val Gly Gln Gln Val Cys Arg Arg Gly Ala225 230 235 240Thr Thr Gly Val Arg Cys Gly Gln Val Ile Ala Leu Asn Ala Thr Val
245 250 255Asn Tyr Gly Gly Gly Asp Val Val Ser Gly Leu Ile Gln Thr Asn Ile
260 265 270Cys Ala Glu Pro Gly Asp Ser Gly Gly Pro Leu Tyr Ala Gly Asp Lys
275 280 285Ile Ile Gly Ile Leu Ser Gly Gly Ser Gly Asp Cys Ala Thr Gly Gly
290 295 300Thr Thr Phe Tyr Gln Pro Ile Gln Glu Val Leu Ser Ala Tyr Gly Leu305 310 315 320Thr Val Tyr<210>39<211>1659<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1).. (1659)<223><400>39gtg gca cca acg ccc cgt cat ctg gaa cag gcg gcc ccg acg gcg acc 48Val Ala Pro Thr Pro Arg His Leu Glu Gln Ala Ala Pro Thr Ala Thr1 5 10 15gag tcg gcc gat ccg gcg ctg tcc tgg ccg aag ggc gtc cct gtg ccg 96Glu Ser Ala Asp Pro Ala Leu Ser Trp Pro Lys Gly Val Pro Val Pro
20 25 30ctg gtg gtg tcc ggc cga ggc gcc gcg gcg ctc gcc gcc cag gcg caa 144Leu Val Val Ser Gly Arg Gly Ala Ala Ala Leu Ala Ala Gln Ala Gln
35 40 45cgg cta cgg acc ttc gta gcc gac gag ccg caa ctc gac ttg agc gaa 192Arg Leu Arg Thr Phe Val Ala Asp Glu Pro Gln Leu Asp Leu Ser Glu
50 55 60ctc ggc tac gcg ttg ggt tgt ggt cgg gcg ggg ttg tcg gat cgt ggg 240Leu Gly Tyr Ala Leu Gly Cys Gly Arg Ala Gly Leu Ser Asp Arg Gly65 70 75 80gtg gtg gtg gcg ggt ggt cgt gag gag ttg ttg gtg ggg ttg ggt ggg 288Val Val Val Ala Gly Gly Arg Glu Glu Leu Leu Val Gly Leu Gly Gly
85 90 95ttg gtg cgg ggt gag ggg ggt gtg ggt gtg gtg tcg ggt tcg gtg gtg 336Leu Val Arg Gly Glu Gly Gly Val Gly Val Val Ser Gly Ser Val Val
100 105 110cgt ggt cgg ttg ggg gtg ttg ttt gct ggt cag ggg tgt cag cgg gtg 384Arg Gly Arg Leu Gly Val Leu Phe Ala Gly Gln Gly Cys Gln Arg Val
115 120 125ggg atg ggg cgt ggg ttg tat gag gtg ttc ccg gtg ttc cgg gat gcc 432Gly Met Gly Arg Gly Leu Tyr Glu Val Phe Pro Val Phe Arg Asp Ala
130 135 140ttc gac gcg gtg tgt gag gtg ttg gat cgg gag ttg ggt gcg ggt ggt 480Phe Asp Ala Val Cys Glu Val Leu Asp Arg Glu Leu Gly Ala Gly Gly145 150 155 160gtg gtg ggt tcg gtg cgg gag gtg gtg ttc ggg ggt ggg ggg ttg ttg 528Val Val Gly Ser Val Arg Glu Val Val Phe Gly Gly Gly Gly Leu Leu
165 170 175gag cgg acg gtg ttt gct cag gcg ggg ttg ttc gcc gtg gag gtg ggg 576Glu Arg Thr Val Phe Ala Gln Ala Gly Leu Phe Ala Val Glu Val Gly
180 185 190ttg ttc cgg ttg gtg gag tcg tgg ggt gtg gtg gtg gat gtg gtg ggt 624Leu Phe Arg Leu Val Glu Ser Trp Gly Val Val Val Asp Val Val Gly
195 200 205ggg cat tcg gtg ggt gag gtg acg gcg gcg tat gtg gcg ggt gtg ttg 672Gly His Ser Val Gly Glu Val Thr Ala Ala Tyr Val Ala Gly Val Leu
210 215 220tcg ttg gag gat gcg gcg gtg ttg gtg gcg gcg cgg ggt cgg ttg atg 720Ser Leu Glu Asp Ala Ala Val Leu Val Ala Ala Arg Gly Arg Leu Met225 230 235 240gag gcg ttg ccg gag ggt ggg gcg atg gtg gcg gtg gct gcg ggt gag 768Glu Ala Leu Pro Glu Gly Gly Ala Met Val Ala Val Ala Ala Gly Glu
245 250 255gag gtg gtg cgg cct ttg ctg gtg tcg gcg gtg gat att gcg gcg gtg 816Glu Val Val Arg Pro Leu Leu Val Ser Ala Val Asp Ile Ala Ala Val
260 265 270aac ggg ccc gaa gcg gtg gtg ctc tcc ggt gat gag gag ccg gta cta 864Ash Gly Pro Glu Ala Val Val Leu Ser Gly Asp Glu Glu Pro Val Leu
275 280 285cgg gtt gcg cgc gat ttg tcg gat cag ggg tgt cgg acg agg cgt ttg 912Arg Val Ala Arg Asp Leu Ser Asp Gln Gly Cys Arg Thr Arg Arg Leu
290 295 300gcg gtt tcg cat gcg ttc cat tcc gcc cgt atg gag ccg atg ctg gag 960Ala Val Ser His Ala Phe His Ser Ala Arg Met Glu Pro Met Leu Glu305 310 315 320gag ttc cgg gag gcg atc gcc gat ctg tcg ttc tcg gcg ccg gtg att 1008Glu Phe Arg Glu Ala Ile Ala Asp Leu Ser Phe Ser Ala Pro Val Ile
325 330 335cct ctg gtg tcg aat gtg acc ggg cgg ttg gcg gat gcg gag acc gtg 1056Pro Leu Val Ser Asn Val Thr Gly Arg Leu Ala Asp Ala Glu Thr Val
340 345 350tgt tcg ccg gag tac tgg gtg gag cat gtg cgt tcg gcc gtg cgg ttc 1104Cys Ser Pro Glu Tyr Trp Val Glu His Val Arg Ser Ala Val Arg Phe
355 360 365gcg gac ggt gtg cgg gcg ctc gct gac tac ggt gtg ggc acc tat ctg 1152Ala Asp Gly Val Arg Ala Leu Ala Asp Tyr Gly Val Gly Thr Tyr Leu
370 375 380gag ttg gcg ccg gat gcg gtg ttg tcc gcg atg gtt ggt gat tgt cra 1200Glu Leu Ala Pro Asp Ala Val Leu Ser Ala Met Val Gly Asp Cys Leu385 390 395 400ccg gaa ggg tcg gct gct gag agt gtg gtg gtg ccg tcg ctg cgg cgg 1248Pro Glu Gly Ser Ala Ala Glu Ser Val Val Val Pro Ser Leu Arg Arg
405 410 415gag ggc gac gag ccc cgt gcg ctg atg acc gcc atc gct cag ctg cat 1296Glu Gly Asp Glu Pro Arg Ala Leu Met Thr Ala Ile Ala Gln Leu His
420 425 430gtg gca ggc gta ccc atc gac ttc ggt gcc ctg ttc ggt gcc acg gtt 1344Val Ala Gly Val Pro Ile Asp Phe Gly Ala Leu Phe Gly Ala Thr Val
435 440 445ctg ccc acc cat att tcg gct ctg ccg acg tat gcg ttc cag cgg gag 1392Leu Pro Thr His Ile Ser Ala Leu Pro Thr Tyr Ala Phe Gln Arg Glu
450 455 460cat tac tgg ttg gtg ggg gac ggg cgt gga gcc ggc gat gtg gcg tcc 1440His Tyr Trp Leu Val Gly Asp Gly Arg Gly Ala Gly Asp Val Ala Ser465 470 475 480gcc ggg ctg gcg ggg gtg gag cat cca ttc ctg ggc gcg atg acg gag 1488Ala Gly Leu Ala Gly Val Glu His Pro Phe Leu Gly Ala Met Thr Glu
485 490 495gtg ccc ggg tcg ggt gag gtg ttg ttc tcc tcg cgg ttg tcg ttg ggg 1536Val Pro Gly Ser Gly Glu Val Leu Phe Ser Ser Arg Leu Ser Leu Gly
500 505 510tct cat ccg tgg ctg gcc gat cat gtg gct gcg ggt gcg gtg ttg ttg 1584Ser His Pro Trp Leu Ala Asp His Val Ala Ala Gly Ala Val Leu Leu
515 520 525ccg ggt gcg gcg ttt gtg gag ttg gtg gtg cgg agc tgg acg atg agg 1632Pro Gly Ala Ala Phe Val Glu Leu Val Val Arg Ser Trp Thr Met Arg
530 535 540tgg gct gcg gtg ggg tgg agg agc tgg 1659Trp Ala Ala Val Gly Trp Arg Ser Trp545 550<210>40<211>553<212>PRT<213>Streptomyces hygroscopicus<400>40Val Ala Pro Thr Pro Arg His Leu Glu Gln Ala Ala Pro Thr Ala Thr1 5 10 15Glu Ser Ala Asp Pro Ala Leu Ser Trp Pro Lys Gly Val Pro Val Pro
20 25 30Leu Val Val Ser Gly Arg Gly Ala Ala Ala Leu Ala Ala Gln Ala Gln
35 40 45Arg Leu Arg Thr Phe Val Ala Asp Glu Pro Gln Leu Asp Leu Ser Glu
50 55 60Leu Gly Tyr Ala Leu Gly Cys Gly Arg Ala Gly Leu Ser Asp Arg Gly65 70 75 80Val Val Val Ala Gly Gly Arg Glu Glu Leu Leu Val Gly Leu Gly Gly
85 90 95Leu Val Arg Gly Glu Gly Gly Val Gly Val Val Ser Gly Ser Val Val
100 105 110Arg Gly Arg Leu Gly Val Leu Phe Ala Gly Gln Gly Cys Gln Arg Val
115 120 125Gly Met Gly Arg Gly Leu Tyr Glu Val Phe Pro Val Phe Arg Asp Ala
130 135 140Phe Asp Ala Val Cys Glu Val Leu Asp Arg Glu Leu Gly Ala Gly Gly145 150 155 160Val Val Gly Ser Val Arg Glu Val Val Phe Gly Gly Gly Gly Leu Leu
165 170 175Glu Arg Thr Val Phe Ala Gln Ala Gly Leu Phe Ala Val Glu Val Gly
180 185 190Leu Phe Arg Leu Val Glu Ser Trp Gly Val Val Val Asp Val Val Gly
195 200 205Gly His Ser Val Gly Glu Val Thr Ala Ala Tyr Val Ala Gly Val Leu
210 215 220Ser Leu Glu Asp Ala Ala Val Leu Val Ala Ala Arg Gly Arg Leu Met225 230 235 240Glu Ala Leu Pro Glu Gly Gly Ala Met Val Ala Val Ala Ala Gly Glu
245 250 255Glu Val Val Arg Pro Leu Leu Val Ser Ala Val Asp Ile Ala Ala Val
260 265 270Asn Gly Pro Glu Ala Val Val Leu Ser Gly Asp Glu Glu Pro Val Leu
275 280 285Arg Val Ala Arg Asp Leu Ser Asp Gln Gly Cys Arg Thr Arg Arg Leu
290 295 300Ala Val Ser His Ala Phe His Ser Ala Arg Met Glu Pro Met Leu Glu305 310 315 320Glu Phe Arg Glu Ala Ile Ala Asp Leu Ser Phe Ser Ala Pro Val Ile
325 330 335Pro Leu Val Ser Asn Val Thr Gly Arg Leu Ala Asp Ala Glu Thr Val
340 345 350Cys Ser Pro Glu Tyr Trp Val Glu His Val Arg Ser Ala Val Arg Phe
355 360 365Ala Asp Gly Val Arg Ala Leu Ala Asp Tyr Gly Val Gly Thr Tyr Leu
370 375 380Glu Leu Ala Pro Asp Ala Val Leu Ser Ala Met Val Gly Asp Cys Leu385 390 395 400Pro Glu Gly Ser Ala Ala Glu Ser Val Val Val Pro Ser Leu Arg Arg
405 410 415Glu Gly Asp Glu Pro Arg Ala Leu Met Thr Ala Ile Ala Gln Leu His
420 425 430Val Ala Gly Val Pro Ile Asp Phe Gly Ala Leu Phe Gly Ala Thr Val
435 440 445Leu Pro Thr His Ile Ser Ala Leu Pro Thr Tyr Ala Phe Gln Arg Glu
450 455 460His Tyr Trp Leu Val Gly Asp Gly Arg Gly Ala Gly Asp Val Ala Ser465 470 475 480Ala Gly Leu Ala Gly Val Glu His Pro Phe Leu Gly Ala Met Thr Glu
485 490 495Val Pro Gly Ser Gly Glu Val Leu Phe Ser Ser Arg Leu Ser Leu Gly
500 505 510Ser His Pro Trp Leu Ala Asp His Val Ala Ala Gly Ala Val Leu Leu
515 520 525Pro Gly Ala Ala Phe Val Glu Leu Val Val Arg Ser Trp Thr Met Arg
530 535 540Trp Ala Ala Val Gly Trp Arg Ser Trp545 550<210>41<211>3339<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1)..(3339)<223><400>41gtg ggc gcc gta ccg ctc cag gaa ccg ctc ggc gta ggc ggg gcc gaa 48Val Gly Ala Val Pro Leu Gln Glu Pro Leu Gly Val Gly Gly Ala Glu1 5 10 15cca ggg gtc ctc gtc gat ctc cag ctc gcg ggc ggc cag ggc gat gtc 96Pro Gly Val Leu Val Asp Leu Gln Leu Ala Gly Gly Gln Gly Asp Val
20 25 30ggc gtg gcg gtc ggc gac ccc gag gcg gcc gac gtc gat cac gcc ggt 144Gly Val Ala Val Gly Asp Pro Glu Ala Ala Asp Val Asp His Ala Gly
35 40 45gac ccg gca ggt ccc ggg gtc gag cag gac gtt gtt ggg gca cag gtc 192Asp Pro Ala Gly Pro Gly Val Glu Gln Asp Val Val Gly Ala Gln Val
50 55 60gcc atg gca gac gac cag gtc ctc ctt ctc ggg acg ggt gcg gtc gag 240Ala Met Ala Asp Asp Gln Val Leu Leu Leu Gly Thr Gly Ala Val Glu65 70 75 80ctc cgc cag gag ctg gtc gcc ggt cca ccc ggc ccg ctc ctc ctg cag 288Leu Arg Gln Glu Leu Val Ala Gly Pro Pro Gly Pro Leu Leu Leu Gln
85 90 95gtc gtc gag gtc cac cag gcc ctc ggc gac gtt ccg ccg ggc ctc ggc 336Val Val Glu Val His Gln Ala Leu Gly Asp Val Pro Pro Gly Leu Gly
100 105 110gac cgc cgc gtc gag gcg ccg gtc gaa ggg gca gtc ctc cac ggg cag 384Asp Arg Arg Val Glu Ala Pro Val Glu Gly Ala Val Leu His Gly Gln
115 120 125ctc gtg gag ggc gcg ggc cag ctc cgc cat cgc ctc gac cac ggc gaa 432Leu Val Glu Gly Ala Gly Gln Leu Arg His Arg Leu Asp His Gly Glu
130 135 140ccg ctg gtg ctc ggg cca ctc ctc ggc cgc cgc gac gcc ggg gac ggc 480Pro Leu Val Leu Gly Pro Leu Leu Gly Arg Arg Asp Ala Gly Asp Gly145 150 155 160ctc cgt gac gag cca cgc ggc ggt gtc gtc ggc acc gcg ctc gac gac 528Leu Arg Asp Glu Pro Arg Gly Gly Val Val Gly Thr Ala Leu Asp Asp
165 170 175gcg ggg gac ggg gat cgg gtg gat gtg gtg cag ccg gtg tcg ttt gcg 576Ala Gly Asp Gly Asp Arg Val Asp Val Val Gln Pro Val Ser Phe Ala
180 185 190gtg atg gtg ggg ttg gcg cgg gtg tgg ttg gcg gct ggt gtg gtg ccg 624Val Met Val Gly Leu Ala Arg Val Trp Leu Ala Ala Gly Val Val Pro
195 200 205tcg gtg gtg gtg ggt cat tcg cag ggg gag att gcg gct gcg tgt gtg 672Ser Val Val Val Gly His Ser Gln Gly Glu Ile Ala Ala Ala Cys Val
210 215 220gcg ggt ggg ttg tcg ttg gag gac gcg gtg cgg gtg gtg gtg ttg cgg 720Ala Gly Gly Leu Ser Leu Glu Asp Ala Val Arg Val Val Val Leu Arg225 230 235 240agt cgt gcg gtg gcg gct ggg ctc tcg ggc cgt ggc ggg atg gtg tcg 768Ser Arg Ala Val Ala Ala Gly Leu Ser Gly Arg Gly Gly Met Val Ser
245 250 255ttg gcg gtg ggt gtg gcg gag gcg gag ggg ttg gtt gag cgg tgg tcg 816Leu Ala Val Gly Val Ala Glu Ala Glu Gly Leu Val Glu Arg Trp Ser
260 265 270ggg cgt atc gag gtg gcg gcg gtg aat ggg ccg ttg tcg gtg gtg gtg 864Gly Arg Ile Glu Val Ala Ala Val Asn Gly Pro Leu Ser Val Val Val
275 280 285gct ggt gag ccg gat gcc ttg cgg ggg ttg gtg gcg gag tgt gag ggc 912Ala Gly Glu Pro Asp Ala Leu Arg Gly Leu Val Ala Glu Cys Glu Gly
290 295 300gcg ggg gtg cgg gcg cgg tgg gtt gat gtg gat tac gcc tcg cat acg 960Ala Gly Val Arg Ala Arg Trp Val Asp Val Asp Tyr Ala Ser His Thr305 310 315 320gcg cag gtg gag gcg gtc gag ggg gag ttg gct cgg tcg ttg gcg caa 1008Ala Gln Val Glu Ala Val Glu Gly Glu Leu Ala Arg Ser Leu Ala Gln
325 330 335att cgt ccg gtg tcc tca cgt att ccg ttc ttt tcg acg gtg gag gct 1056Ile Arg Pro Val Ser Ser Arg Ile Pro Phe Phe Ser Thr Val Glu Ala
340 345 350ggg tgg ctg gac acg gcc gag ctg gac gcc ggg tac tgg tac cgg aat 1104Gly Trp Leu Asp Thr Ala Glu Leu Asp Ala Gly Tyr Trp Tyr Arg Asn
355 360 365ctg cgg agc acc gtg cgc ttc gcg ccg tcg atc gac cgg ttg atc gag 1152Leu Arg Ser Thr Val Arg Phe Ala Pro Ser Ile Asp Arg Leu Ile Glu
370 375 380gaa ggc ttt gcg gcg ttt gtc gaa gtg agc gcg cat ccg gtg ctg acg 1200Glu Gly Phe Ala Ala Phe Val Glu Val Ser Ala His Pro Val Leu Thr385 390 395 400atg ggc atc gag gcg gcg gcg gag cgg gcg gac gtt ggg ccg gtc gtg 1248Met Gly Ile Glu Ala Ala Ala Glu Arg Ala Asp Val Gly Pro Val Val
405 410 415gtg acc ggg acg ctc cgc cgg gat cag ggt gat atg cgt cgt gtg ctc 1296Val Thr Gly Thr Leu Arg Arg Asp Gln Gly Asp Met Arg Arg Val Leu
420 425 430act tcc ctg gcc gag gtg tac gta cgc ggt gtc ccc gtg aac tgg acc 1344Thr Ser Leu Ala Glu Val Tyr Val Arg Gly Val Pro Val Asn Trp Thr
435 440 445acc ctg ctg ggc gac atc ccg gcg cgc gcc gcg ttg gat ctg ccg acg 1392Thr Leu Leu Gly Asp Ile Pro Ala Arg Ala Ala Leu Asp Leu Pro Thr
450 455 460tac gcc ttc cag cat cag cac tac tgg ctg aag aac gcc att ccc acc 1440Tyr Ala Phe Gln His Gln His Tyr Trp Leu Lys Asn Ala Ile Pro Thr465 470 475 480gat gcg gga gcc atc gac gat cag ctt ccg ggc ctg gtc gaa ctg ccc 1488Asp Ala Gly Ala Ile Asp Asp Gln Leu Pro Gly Leu Val Glu Leu Pro
485 490 495gcc gag acc ggc gcc ttg acc gct cgc ctt ctt ggg gag tcc acg cag 1536Ala Glu Thr Gly Ala Leu Thr Ala Arg Leu Leu Gly Glu Ser Thr Gln
500 505 510gaa cag gaa cgc atc ctg ctc aag acc gtt cgc cag gag acc gcg agc 1584Glu Gln Glu Arg Ile Leu Leu Lys Thr Val Arg Gln Glu Thr Ala Ser
515 520 525gtc ttg ggc cac tcc tcg ctg gac gcc att gaa ccg gac atg gtg ttc 1632Val Leu Gly His Ser Ser Leu Asp Ala Ile Glu Pro Asp Met Val Phe
530 535 540aac cag atc ggc ttc gac tcg gcc acc gca gta cag ctg cga aac cgt 1680Asn Gln Ile Gly Phe Asp Ser Ala Thr Ala Val Gln Leu Arg Asn Arg545 550 555 560ctg aac gcg ctc acc gac cgg act ctg ccg acc acc ctg ctc ttc gac 1728Leu Asn Ala Leu Thr Asp Arg Thr Leu Pro Thr Thr Leu Leu Phe Asp
565 570 575tac ccc acg ccc ctg atc ctc gcc gac ttc ctg cgt gac gaa ctc atc 1776Tyr Pro Thr Pro Leu Ile Leu Ala Asp Phe Leu Arg Asp Glu Leu Ile
580 585 590ggg gac acg gcg gcc ccg gag ggg gtg ccg gaa gcg aca gcg gcg ccg 1824Gly Asp Thr Ala Ala Pro Glu Gly Val Pro Glu Ala Thr Ala Ala Pro
595 600 605ggg gat gtg tcg acc gag ccg gtg gcg atc gtg ggt atg gcg tgc cgg 1872Gly Asp Val Ser Thr Glu Pro Val Ala Ile Val Gly Met Ala Cys Arg
610 615 620ctg ccg ggt ggc gtc tcc acc ccg gaa gag cta tgg gac ctg gtg ctt 1920Leu Pro Gly Gly Val Ser Thr Pro Glu Glu Leu Trp Asp Leu Val Leu625 630 635 640cag ggg cgg gac ggg gtc agc gac ttc ccc gtg aac cgt ggc tgg gat 1968Gln Gly Arg Asp Gly Val Ser Asp Phe Pro Val Asn Arg Gly Trp Asp
645 650 655ctg gag aat ctg ttc cac ccg gac ccg gac cac ccc gct acc agc tat 2016Leu Glu Asn Leu Phe His Pro Asp Pro Asp His Pro Ala Thr Ser Tyr
660 665 670gcg cac caa ggc gga ttt ctg cac gac gcc ggg gag ttt gac gcg ggt 2064Ala His Gln Gly Gly Phe Leu His Asp Ala Gly Glu Phe Asp Ala Gly
675 680 685ttc ttc ggg atc tca cca cgc gag gca ctg gcc gtg gac ccg caa cag 2112Phe Phe Gly Ile Ser Pro Arg Glu Ala Leu Ala Val Asp Pro Gln Gln
690 695 700cgt ctg atg ctg gaa acc tcg tgg gaa gcg ctg gaa cgc gcc ggg atc 2160Arg Leu Met Leu Glu Thr Ser Trp Glu Ala Leu Glu Arg Ala Gly Ile705 710 715 720gac ccg acc acg ctg cgg ggc aag gac gtc ggt gtc ttc tcc ggt gtg 2208Asp Pro Thr Thr Leu Arg Gly Lys Asp Val Gly Val Phe Ser Gly Val
725 730 735acg tac cac aac tac ggc tcg ggc gtg gag ccg gtt ccc gcc gag ctc 2256Thr Tyr His Asn Tyr Gly Ser Gly Val Glu Pro Val Pro Ala Glu Leu
740 745 750gaa ggc atg ctg ggg ctc ggc gcc tcg gcg agc gtg ctg tca ggg cgg 2304Glu Gly Met Leu Gly Leu Gly Ala Ser Ala Ser Val Leu Ser Gly Arg
755 760 765gtg tcg tat gcg ctg ggc ttc gag ggg ccg tcg gtc gcg gtg gac acg 2352Val Ser Tyr Ala Leu Gly Phe Glu Gly Pro Ser Val Ala Val Asp Thr
770 775 780gcg tgc tcc tcg tcc ctg gtg gcg ttg cac ttg gcg gcg cag gcg ttg 2400Ala Cys Ser Ser Ser Leu Val Ala Leu His Leu Ala Ala Gln Ala Leu785 790 795 800cga gca ggc gag tgc tcg atc gcc ctt gcc ggt ggg gtc acg gtg atg 2448Arg Ala Gly Glu Cys Ser Ile Ala Leu Ala Gly Gly Val Thr Val Met
805 810 815ccg act ccc ggt atc ttc atc gcc ttc tca cgg cag cgc ggc atg tcg 2496Pro Thr Pro Gly Ile Phe Ile Ala Phe Ser Arg Gln Arg Gly Met Ser
820 825 830gtc gat ggc cgg tgc aag tcg ttc tcg gcg tcg gcg gac ggt acg ggg 2544Val Asp Gly Arg Cys Lys Ser Phe Ser Ala Ser Ala Asp Gly Thr Gly
835 840 845tgg gcc gag ggt gtg ggt gtg ctg gcg ctg gag cgg ctg tcg gac gcg 2592Trp Ala Glu Gly Val Gly Val Leu Ala Leu Glu Arg Leu Ser Asp Ala
850 855 860gag cga aac ggc cat cgg gtg ttg gcg gtg gtg cgg ggc agt gcg gtg 2640Glu Arg Asn Gly His Arg Val Leu Ala Val Val Arg Gly Ser Ala Val865 870 875 880aat cag gac ggt gcg tcg aat ggg ttg acg gcg ccg aat ggt ccg tcg 2688Asn Gln Asp Gly Ala Ser Asn Gly Leu Thr Ala Pro Asn Gly Pro Ser
885 890 895cag cag cgt gtc att cgg cag gcg ctg gcc agt gcg ggt gtg tcg gct 2736Gln Gln Arg Val Ile Arg Gln Ala Leu Ala Set Ala Gly Val Ser Ala
900 905 910gcc gag gtg gat gtg gtc gag gca cat ggc acg ggt acg gcg ctg ggc 2784Ala Glu Val Asp Val Val Glu Ala His Gly Thr Gly Thr Ala Leu Gly
915 920 925gat ccc att gag gcg cag gcg gtg ttg gcc acg tat ggc cag gat cgt 2832Asp Pro Ile Glu Ala Gln Ala Val Leu Ala Thr Tyr Gly Gln Asp Arg
930 935 940gat cgg cct ttg ttg atg ggg tcg ttg aag tcg aat atc ggt cat gcg 2880Asp Arg Pro Leu Leu Met Gly Ser Leu Lys Ser Asn Ile Gly His Ala945 950 955 960cag gcg gcc gcg ggt gtg gct ggt gtg atc aag atg gtg ttg gcg ctg 2928Gln Ala Ala Ala Gly Val Ala Gly Val Ile Lys Met Val Leu Ala Leu
965 970 975cgg cat ggc atc gct cct cgg acg ttg cat gtg gac gag ccg acc tcg 2976Arg His Gly Ile Ala Pro Arg Thr Leu His Val Asp Glu Pro Thr Ser
980 985 990cag gtg gat tgg tcg acg ggt gcg gtg gag ctg ttg acc gag gag cgg 3024Gln Val Asp Trp Ser Thr Gly Ala Val Glu Leu Leu Thr Glu Glu Arg
995 1000 1005gtg tgg cct gag gtg ggt cgt cct cgc cgg gct gga gtg tcc gcg 3069Val Trp Pro Glu Val Gly Arg Pro Arg Arg Ala Gly Val Ser Ala
1010 1015 1020ttc ggg gtc agt ggc acc aac gcc ccg tca tct gga aca ggc ggc 3114Phe Gly Val Ser Gly Thr Asn Ala Pro Ser Ser Gly Thr Gly Gly
1025 1030 1035ccc gac ggc gac cga gtc ggc cga tcc ggc gct gtc ctg gcc gaa 3159Pro Asp Gly Asp Arg Val Gly Arg Ser Gly Ala Val Leu Ala Glu
1040 1045 1050ggg cgt ccc tgt gcc gct ggt ggt gtc cgg ccg agg cgc cgc ggc 3204Gly Arg Pro Cys Ala Ala Gly Gly Val Arg Pro Arg Arg Arg Gly
1055 1060 1065gct cgc cgc cca ggc gca acg gct acg gac ctt cgt agc cga cga 3249Ala Arg Arg Pro Gly Ala Thr Ala Thr Asp Leu Arg Ser Arg Arg
1070 1075 1080gcc gca act cga ctt gag cga act cgg cta cgc gtt ggg ttg tgg 3294Ala Ala Thr Arg Leu Glu Arg Thr Arg Leu Arg Val Gly Leu Trp
1085 1090 1095tcg ggc ggg gtt gtc gga tcg tgg ggt ggt ggt ggc ggg tgg tcg 3339Ser Gly Gly Val Val Gly Ser Trp Gly Gly Gly Gly Gly Trp Ser
1100 1105 1110<210>42<211>1113<212>PRT<213>Streptomyces hygroscopicus<400>42Val Gly Ala Val Pro Leu Gln Glu Pro Leu Gly Val Gly Gly Ala Glu1 5 10 15Pro Gly Val Leu Val Asp Leu Gln Leu Ala Gly Gly Gln Gly Asp Val
20 25 30Gly Val Ala Val Gly Asp Pro Glu Ala Ala Asp Val Asp His Ala Gly
35 40 45Asp Pro Ala Gly Pro Gly Val Glu Gln Asp Val Val Gly Ala Gln Val
50 55 60Ala Met Ala Asp Asp Gln Val Leu Leu Leu Gly Thr Gly Ala Val Glu65 70 75 80Leu Arg Gln Glu Leu Val Ala Gly Pro Pro Gly Pro Leu Leu Leu Gln
85 90 95Val Val Glu Val His Gln Ala Leu Gly Asp Val Pro Pro Gly Leu Gly
100 105 110Asp Arg Arg Val Glu Ala Pro Val Glu Gly Ala Val Leu His Gly Gln
115 120 125Leu Val Glu Gly Ala Gly Gln Leu Arg His Arg Leu Asp His Gly Glu
130 135 140Pro Leu Val Leu Gly Pro Leu Leu Gly Arg Arg Asp Ala Gly Asp Gly145 150 155 160Leu Arg Asp Glu Pro Arg Gly Gly Val Val Gly Thr Ala Leu Asp Asp
165 170 175Ala Gly Asp Gly Asp Arg Val Asp Val Val Gln Pro Val Ser Phe Ala
180 185 190Val Met Val Gly Leu Ala Arg Val Trp Leu Ala Ala Gly Val Val Pro
195 200 205Ser Val Val Val Gly His Ser Gln Gly Glu Ile Ala Ala Ala Cys Val
210 215 220Ala Gly Gly Leu Ser Leu Glu Asp Ala Val Arg Val Val Val Leu Arg225 230 235 240Ser Arg Ala Val Ala Ala Gly Leu Ser Gly Arg Gly Gly Met Val Ser
245 250 255Leu Ala Val Gly Val Ala Glu Ala Glu Gly Leu Val Glu Arg Trp Ser
260 265 270Gly Arg Ile Glu Val Ala Ala Val Asn Gly Pro Leu Ser Val Val Val
275 280 285Ala Gly Glu Pro Asp Ala Leu Arg Gly Leu Val Ala Glu Cys Glu Gly
290 295 300Ala Gly Val Arg Ala Arg Trp Val Asp Val Asp Tyr Ala Ser His Thr305 310 315 320Ala Gln Val Glu Ala Val Glu Gly Glu Leu Ala Arg Ser Leu Ala Gln
325 330 335Ile Arg Pro Val Ser Ser Arg Ile Pro Phe Phe Ser Thr Val Glu Ala
340 345 350Gly Trp Leu Asp Thr Ala Glu Leu Asp Ala Gly Tyr Trp Tyr Arg Asn
355 360 365Leu Arg Ser Thr Val Arg Phe Ala Pro Ser Ile Asp Arg Leu Ile Glu
370 375 380Glu Gly Phe Ala Ala Phe Val Glu Val Ser Ala His Pro Val Leu Thr385 390 395 400Met Gly Ile Glu Ala Ala Ala Glu Arg Ala Asp Val Gly Pro Val Val
405 410 415Val Thr Gly Thr Leu Arg Arg Asp Gln Gly Asp Met Arg Arg Val Leu
420 425 430Thr Ser Leu Ala Glu Val Tyr Val Arg Gly Val Pro Val Asn Trp Thr
435 440 445Thr Leu Leu Gly Asp Ile Pro Ala Arg Ala Ala Leu Asp Leu Pro Thr
450 455 460Tyr Ala Phe Gln His Gln His Tyr Trp Leu Lys Asn Ala Ile Pro Thr465 470 475 480Asp Ala Gly Ala Ile Asp Asp Gln Leu Pro Gly Leu Val Glu Leu Pro
485 490 495Ala Glu Thr Gly Ala Leu Thr Ala Arg Leu Leu Gly Glu Ser Thr Gln
500 505 510Glu Gln Glu Arg Ile Leu Leu Lys Thr Val Arg Gln Glu Thr Ala Ser
515 520 525Val Leu Gly His Ser Ser Leu Asp Ala Ile Glu Pro Asp Met Val Phe
530 535 540Asn Gln Ile Gly Phe Asp Ser Ala Thr Ala Val Gln Leu Arg Asn Arg545 550 555 560Leu Asn Ala Leu Thr Asp Arg Thr Leu Pro Thr Thr Leu Leu Phe Asp
565 570 575Tyr Pro Thr Pro Leu Ile Leu Ala Asp Phe Leu Arg Asp Glu Leu Ile
580 585 590Gly Asp Thr Ala Ala Pro Glu Gly Val Pro Glu Ala Thr Ala Ala Pro
595 600 605Gly Asp Val Ser Thr Glu Pro Val Ala Ile Val Gly Met Ala Cys Arg
610 615 620Leu Pro Gly Gly Val Ser Thr Pro Glu Glu Leu Trp Asp Leu Val Leu625 630 635 640Gln Gly Arg Asp Gly Val Ser Asp Phe Pro Val Asn Arg Gly Trp Asp
645 650 655Leu Glu Asn Leu Phe His Pro Asp Pro Asp His Pro Ala Thr Ser Tyr
660 665 670Ala His Gln Gly Gly Phe Leu His Asp Ala Gly Glu Phe Asp Ala Gly
675 680 685Phe Phe Gly Ile Ser Pro Arg Glu Ala Leu Ala Val Asp Pro Gln Gln
690 695 700Arg Leu Met Leu Glu Thr Ser Trp Glu Ala Leu Glu Arg Ala Gly Ile705 710 715 720Asp Pro Thr Thr Leu Arg Gly Lys Asp Val Gly Val Phe Ser Gly Val
725 730 735Thr Tyr His Asn Tyr Gly Ser Gly Val Glu Pro Val Pro Ala Glu Leu
740 745 750Glu Gly Met Leu Gly Leu Gly Ala Ser Ala Ser Val Leu Ser Gly Arg
755 760 765Val Ser Tyr Ala Leu Gly Phe Glu Gly Pro Ser Val Ala Val Asp Thr
770 775 780Ala Cys Ser Ser Ser Leu Val Ala Leu His Leu Ala Ala Gln Ala Leu785 790 795 800Arg Ala Gly Glu Cys Ser Ile Ala Leu Ala Gly Gly Val Thr Val Met
805 810 815Pro Thr Pro Gly Ile Phe Ile Ala Phe Ser Arg Gln Arg Gly Met Ser
820 825 830Val Asp Gly Arg Cys Lys Ser Phe Ser Ala Ser Ala Asp Gly Thr Gly
835 840 845Trp Ala Glu Gly Val Gly Val Leu Ala Leu Glu Arg Leu Ser Asp Ala
850 855 860Glu Arg Asn Gly His Arg Val Leu Ala Val Val Arg Gly Ser Ala Val865 870 875 880Asn Gln Asp Gly Ala Ser Asn Gly Leu Thr Ala Pro Asn Gly Pro Ser
885 890 895Gln Gln Arg Val Ile Arg Gln Ala Leu Ala Ser Ala Gly Val Ser Ala
900 905 910Ala Glu Val Asp Val Val Glu Ala His Gly Thr Gly Thr Ala Leu Gly
915 920 925Asp Pro Ile Glu Ala Gln Ala Val Leu Ala Thr Tyr Gly Gln Asp Arg
930 935 940Asp Arg Pro Leu Leu Met Gly Ser Leu Lys Ser Asn Ile Gly His Ala945 950 955 960Gln Ala Ala Ala Gly Val Ala Gly Val Ile Lys Met Val Leu Ala Leu
965 970 975Arg His Gly Ile Ala Pro Arg Thr Leu His Val Asp Glu Pro Thr Ser
980 985 990Gln Val Asp Trp Ser Thr Gly Ala Val Glu Leu Leu Thr Glu Glu Arg
995 1000 1005Val Trp Pro Glu Val Gly Arg Pro Arg Arg Ala Gly Val Ser Ala
1010 1015 1020Phe Gly Val Ser Gly Thr Asn Ala Pro Ser Ser Gly Thr Gly Gly
1025 1030 1035Pro Asp Gly Asp Arg Val Gly Arg Ser Gly Ala Val Leu Ala Glu
1040 1045 1050Gly Arg Pro Cys Ala Ala Gly Gly Val Arg Pro Arg Arg Arg Gly
1055 1060 1065Ala Arg Arg Pro Gly Ala Thr Ala Thr Asp Leu Arg Ser Arg Arg
1070 1075 1080Ala Ala Thr Arg Leu Glu Arg Thr Arg Leu Arg Val Gly Leu Trp
1085 1090 1095Ser Gly Gly Val Val Gly Ser Trp Gly Gly Gly Gly Gly Trp Ser
1100 1105 1110<210>43<211>1479<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1)..(1479)<223><400>43gtg gcc gct gct gcc gcg ctg agc gcg tgc ggc aca ccg gaa gca cac 48Val Ala Ala Ala Ala Ala Leu Ser Ala Cys Gly Thr Pro Glu Ala His 1 5 10 15gga aga ccc acg ggg gtg gcg atg gag ccg gcg gca ccg gcg cag tac 96Gly Arg Pro Thr Gly Val Ala Met Glu Pro Ala Ala Pro Ala Gln Tyr
20 25 30gta ctg atc act cag tgc ttg cag aac gac ttc ttc ctc aac ctg gac 144Val Leu Ile Thr Gln Cys Leu Gln Asn Asp Phe Phe Leu Asn Leu Asp
35 40 45tgc cag ttg tcc ctg ccg gac agc gcc gtc tcc aag ctg ctg ctg gac 192Cys Gln Leu Ser Leu Pro Asp Ser Ala Val Ser Lys Leu Leu Leu Asp
50 55 60agc gag agc ggt gcg tcc ctc cac acg gag ggc cac cgc agg gtc ctg 240Ser Glu Ser Gly Ala Ser Leu His Thr Glu Gly His Arg Arg Val Leu65 70 75 80tcc gag tcg gag ctg cgc cgt tcg ccc ctg gcc cgt ttc ctc gac gcc 288Ser Glu Ser Glu Leu Arg Arg Ser Pro Leu Ala Arg Phe Leu Asp Ala
85 90 95acc gtg ggc tcc cgt acg cgc gga cac ggg gac ggg gtt ctg cat ctg 336Thr Val Gly Ser Arg Thr Arg Gly His Gly Asp Gly Val Leu His Leu
100 105 110atc aac atc cgt gac tgg cat gtc ccg gga gag aca tac gac ctg gag 384Ile Asn Ile Arg Asp Trp His Val Pro Gly Glu Thr Tyr Asp Leu Glu
115 120 125cgc agg cag tac ggg gcc cat tgc gag gcc gac acc tgg ggg gcg gcg 432Arg Arg Gln Tyr Gly Ala His Cys Glu Ala Asp Thr Trp Gly Ala Ala
130 135 140tac gtc gac ggg ctc acg gac ctg ctg gcc ccg gat gag cgc gcg ccc 480Tyr Val Asp Gly Leu Thr Asp Leu Leu Ala Pro Asp Glu Arg Ala Pro145 150 155 160gcg gac ggc gag ggc ggc tgg ggc ggg aaa ctc cat gtc cac cat gtg 528Ala Asp Gly Glu Gly Gly Trp Gly Gly Lys Leu His Val His His Val
165 170 175cgg tcc aac acc ctc ttc gac ttc cag cac agc gcc ggc gga cgt ccc 576Arg Ser Asn Thr Leu Phe Asp Phe Gln His Ser Ala Gly Gly Arg Pro
180 185 190gac ctc agc gaa ccg gcg ccg ctg acc aca ctg ctg gac ggt ctg ctg 624Asp Leu Ser Glu Pro Ala Pro Leu Thr Thr Leu Leu Asp Gly Leu Leu
195 200 205ggc gat gga cgt cag gag acg acg cat gtc gcg gtg gtc ggc gtc ctc 672Gly Asp Gly Arg Gln Glu Thr Thr His Val Ala Val Val Gly Val Leu
210 215 220acc gat atc aag gtc cag ctg ctg ctg acc ggt atc cgc tcc cgc tac 720Thr Asp Ile Lys Val Gln Leu Leu Leu Thr Gly Ile Arg Ser Arg Tyr225 230 235 240gac gta cgg cag ctc gtc gtc tcc gac gcg ctc acc gcc agc agg acc 768Asp Val Arg Gln Leu Val Val Ser Asp Ala Leu Thr Ala Ser Arg Thr
245 250 255ctg gag cgc cat ctg acg gcg ctg gac ttc tgc cag cgt gtg ctg cgc 816Leu Glu Arg His Leu Thr Ala Leu Asp Phe Cys Gln Arg Val Leu Arg
260 265 270acc gag gtg atg atc ggc ctg gcg gag ctg gcc cgt ttc ctg ggc tcc 864Thr Glu Val Met Ile Gly Leu Ala Glu Leu Ala Arg Phe Leu Gly Ser
275 280 285cgg ccg gac gac gac cgg ctg tcc cgt ggc ggg gac gag gag ttc gtg 912Arg Pro Asp Asp Asp Arg Leu Ser Arg Gly Gly Asp Glu Glu Phe Val
290 295 300ggc tac tcc tca tac atc cag gac aag cag ggc atc ctg tcg tac gag 960Gly Tyr Ser Ser Tyr Ile Gln Asp Lys Gln Gly Ile Leu Ser Tyr Glu305 310 315 320gac gcc agg atg cgg gac tat cgc atc cag acc tcg gaa cgc ctc cgg 1008Asp Ala Arg Met Arg Asp Tyr Arg Ile Gln Thr Ser Glu Arg Leu Arg
325 330 335cgg acg cag cac acg gtc ggc ttc gcc agc aag ttc ctg ctg ggg ctg 1056Arg Thr Gln His Thr Val Gly Phe Ala Ser Lys Phe Leu Leu Gly Leu
340 345 350ggc acc tgt ctg ctg gtc agc gcc ctt gtg ctg tcg ctc gtc ggc gtc 1104Gly Thr Cys Leu Leu Val Ser Ala Leu Val Leu Ser Leu Val Gly Val
355 360 365ttc ctc ccg ggc cgt atc ggg tgg cag agc ccc gcc gtg ctc ggg gcg 1152Phe Leu Pro Gly Arg Ile Gly Trp Gln Ser Pro Ala Val Leu Gly Ala
370 375 380ctc ggg gtg ggg cag atc gtc acg ttg ttc ttc acc cgg ccg gtc aga 1200Leu Gly Val Gly Gln Ile Val Thr Leu Phe Phe Thr Arg Pro Val Arg385 390 395 400tcg gtg cag gac gcg ctg gcg gag gag acc atc tac cgg atg atc ctg 1248Ser Val Gln Asp Ala Leu Ala Glu Glu Thr Ile Tyr Arg Met Ile Leu
405 410 415gag agc cgc agc ctg aag gtg gcc ctg gcg cgg ttc cac atc acc acg 1296Glu Ser Arg Ser Leu Lys Val Ala Leu Ala Arg Phe His Ile Thr Thr
420 425 430gcc acc gcg ctc cga cgg cat gac gat gtg aac ggc caa tcc gac gcc 1344Ala Thr Ala Leu Arg Arg His Asp Asp Val Asn Gly Gln Ser Asp Ala
435 440 445ctg gca cgc cag ttg gag atc ctg gag aag atc gac acg gcc gac ttc 1392Leu Ala Arg Gln Leu Glu Ile Leu Glu Lys Ile Asp Thr Ala Asp Phe
450 455 460gaa cgg ctg aaa cag ctg ggg gtg acc ccg cgg gcc gaa tcg tcc ggg 1440Glu Arg Leu Lys Gln Leu Gly Val Thr Pro Arg Ala Glu Ser Ser Gly465 470 475 480acg ggg cgg ccc cgc aga aga atc cgc tca cag gtt tcc 1479Thr Gly Arg Pro Arg Arg Arg Ile Arg Ser Gln Val Ser
485 490<210>44<211>493<212>PRT<213>Streptomyces hygroscopicus<400>44Val Ala Ala Ala Ala Ala Leu Ser Ala Cys Gly Thr Pro Glu Ala His1 5 10 15Gly Arg Pro Thr Gly Val Ala Met Glu Pro Ala Ala Pro Ala Gln Tyr
20 25 30Val Leu Ile Thr Gln Cys Leu Gln Asn Asp Phe Phe Leu Asn Leu Asp
35 40 45Cys Gln Leu Ser Leu Pro Asp Ser Ala Val Ser Lys Leu Leu Leu Asp
50 55 60Ser Glu Ser Gly Ala Ser Leu His Thr Glu Gly His Arg Arg Val Leu65 70 75 80Ser Glu Ser Glu Leu Arg Arg Ser Pro Leu Ala Arg Phe Leu Asp Ala
85 90 95Thr Val Gly Ser Arg Thr Arg Gly His Gly Asp Gly Val Leu His Leu
100 105 110Ile Asn Ile Arg Asp Trp His Val Pro Gly Glu Thr Tyr Asp Leu Glu
115 120 125Arg Arg Gln Tyr Gly Ala His Cys Glu Ala Asp Thr Trp Gly Ala Ala
130 135 140Tyr Val Asp Gly Leu Thr Asp Leu Leu Ala Pro Asp Glu Arg Ala Pro145 150 155 160Ala Asp Gly Glu Gly Gly Trp Gly Gly Lys Leu His Val His His Val
165 170 175Arg Ser Asn Thr Leu Phe Asp Phe Gln His Ser Ala Gly Gly Arg Pro
180 185 190Asp Leu Ser Glu Pro Ala Pro Leu Thr Thr Leu Leu Asp Gly Leu Leu
195 200 205Gly Asp Gly Arg Gln Glu Thr Thr His Val Ala Val Val Gly Val Leu
210 215 220Thr Asp Ile Lys Val Gln Leu Leu Leu Thr Gly Ile Arg Ser Arg Tyr225 230 235 240Asp Val Arg Gln Leu Val Val Ser Asp Ala Leu Thr Ala Ser Arg Thr
245 250 255Leu Glu Arg His Leu Thr Ala Leu Asp Phe Cys Gln Arg Val Leu Arg
260 265 270Thr Glu Val Met Ile Gly Leu Ala Glu Leu Ala Arg Phe Leu Gly Ser
275 280 285Arg Pro Asp Asp Asp Arg Leu Ser Arg Gly Gly Asp Glu Glu Phe Val
290 295 300Gly Tyr Ser Ser Tyr Ile Gln Asp Lys Gln Gly Ile Leu Ser Tyr Glu305 310 315 320Asp Ala Arg Met Arg Asp Tyr Arg Ile Gln Thr Ser Glu Arg Leu Arg
325 330 335Arg Thr Gln His Thr Val Gly Phe Ala Ser Lys Phe Leu Leu Gly Leu
340 345 350Gly Thr Cys Leu Leu Val Ser Ala Leu Val Leu Ser Leu Val Gly Val
355 360 365Phe Leu Pro Gly Arg Ile Gly Trp Gln Ser Pro Ala Val Leu Gly Ala
370 375 380Leu Gly Val Gly Gln Ile Val Thr Leu Phe Phe Thr Arg Pro Val Arg385 390 395 400Ser Val Gln Asp Ala Leu Ala Glu Glu Thr Ile Tyr Arg Met Ile Leu
405 410 415Glu Ser Arg Ser Leu Lys Val Ala Leu Ala Arg Phe His Ile Thr Thr
420 425 430Ala Thr Ala Leu Arg Arg His Asp Asp Val Asn Gly Gln Ser Asp Ala
435 440 445Leu Ala Arg Gln Leu Glu Ile Leu Glu Lys Ile Asp Thr Ala Asp Phe
450 455 460Glu Arg Leu Lys Gln Leu Gly Val Thr Pro Arg Ala Glu Ser Ser Gly465 470 475 480Thr Gly Arg Pro Arg Arg Arg Ile Arg Ser Gln Val Ser
485 490<210>45<211>1521<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1)..(1521)<223><220><221>misc_feature<222>(48,431)<223>R=a or g<400>45atg tca aca cca cct atc gcc ggt gac gac cag acg ccg cgc cgg ctr 48Met Ser Thr Pro Pro Ile Ala Gly Asp Asp Gln Thr Pro Arg Arg Xaa1 5 10 15agg ctg cgc cgc cgc agg gcc gac gcc gac cgc ggg cgc cgg ggc ggg 96Arg Leu Arg Arg Arg Arg Ala Asp Ala Asp Arg Gly Arg Arg Gly Gly
20 25 30cgg gca tcc gca cgg cgg ttc ccc gat ggc gcc ctg ccg cag ccc gag 144Arg Ala Ser Ala Arg Arg Phe Pro Asp Gly Ala Leu Pro Gln Pro Glu
35 40 45ccc gtc gcg tcg gat gtc atc cgg gcc ggt gac agc acc tgg ctc cgg 192Pro Val Ala Ser Asp Val Ile Arg Ala Gly Asp Ser Thr Trp Leu Arg
50 55 60gac cgg gcg cgc aag cac ggc gcg tcg gcg gcc acc cgg aag gtc ttc 240Asp Arg Ala Arg Lys His Gly Ala Ser Ala Ala Thr Arg Lys Val Phe65 70 75 80gac ccc tgg gtc ctg gcc ggc ccg gac cgg gtg ccc tac ttc gcc gaa 288Asp Pro Trp Val Leu Ala Gly Pro Asp Arg Val Pro Tyr Phe Ala Glu
85 90 95ctg gcc agt ctg cgc aac cgg gtc aag cac cgg ctc gcc gag gag cat 336Leu Ala Ser Leu Arg Asn Arg Val Lys His Arg Leu Ala Glu Glu His
100 105 110gcg cga gcc gag gag gac ggc gcg ctg gag gcg agc cgg gtc agg gcg 384Ala Arg Ala Glu Glu Asp Gly Ala Leu Glu Ala Ser Arg Val Arg Ala
115 120 125gcc gcc acc gcg gcc gga gag cgg ctg gag cgg gcc ggg cag cgg crg 432Ala Ala Thr Ala Ala Gly Glu Arg Leu Glu Arg Ala Gly Gln Arg Xaa
130 135 140gtc gtc ctg gag cgg cag cag acg gtc acc acc gcc cag ctg gac cgg 480Val Val Leu Glu Arg Gln Gln Thr Val Thr Thr Ala Gln Leu Asp Arg145 150 155 160ctg gcg cgg cgg gcc gac cgg tgg cag acc ttc cgc gac acc gtg cgg 528Leu Ala Arg Arg Ala Asp Arg Trp Gln Thr Phe Arg Asp Thr Val Arg
165 170 175ggc ggt ttc gag cgc cgg tgg ctg cgc gcc cgt atg cct gcc gac ggc 576Gly Gly Phe Glu Arg Arg Trp Leu Arg Ala Arg Met Pro Ala Asp Gly
180 185 190agc gac ggg acc gac ccc gga cgg cag ggc gcc acc cgg cgc gag gac 624Ser Asp Gly Thr Asp Pro Gly Arg Gln Gly Ala Thr Arg Arg Glu Asp
195 200 205gag ccg gag acc acc ggc cac gcc agc tgg cag gcg gtg tcc gaa ccc 672Glu Pro Glu Thr Thr Gly His Ala Ser Trp Gln Ala Val Ser Glu Pro
210 215 220gat ccg gtg gcg gag gcg gac gcg gcc gac cgc gcc ctg tcc acc agg 720Asp Pro Val Ala Glu Ala Asp Ala Ala Asp Arg Ala Leu Ser Thr Arg225 230 235 240gcg gcg tgg gag ggc gcg gcg gcg cgc ccc ggg atg ccg cgc tgg atg 768Ala Ala Trp Glu Gly Ala Ala Ala Arg Pro Gly Met Pro Arg Trp Met
245 250 255aag ctc ggt gtg ctg gcc gcg ttg gtc gtg gtg gaa ctg ccc gtc tac 816Lys Leu Gly Val Leu Ala Ala Leu Val Val Val Glu Leu Pro Val Tyr
260 265 270tac tcg gtg ttc gag aat ctg cac ggt gtc ggg cgc ttc gcc gat ctg 864Tyr Ser Val Phe Glu Asn Leu His Gly Val Gly Arg Phe Ala Asp Leu
275 280 285ctc tcc tac agc ctc atg gtg gcc gtg gcg gtg gcg atg atc ctc gcc 912Leu Ser Tyr Ser Leu Met Val Ala Val Ala Val Ala Met Ile Leu Ala
290 295 300ccg cat atc gcg ggc tgg ata ctg cgg cgg cgc tcc gcc acc ggt gcg 960Pro His Ile Ala Gly Trp Ile Leu Arg Arg Arg Ser Ala Thr Gly Ala305 310 315 320gtc cgg ctg tcg gcc gtg ccc gcc ctc gcc ctg ctg ggc gtg tgg gcg 1008Val Arg Leu Ser Ala Val Pro Ala Leu Ala Leu Leu Gly Val Trp Ala
325 330 335tac ggc gcc tgg gcg ctg ggg gat ctg cgg gcc aag gtg gcg ttc cgg 1056Tyr Gly Ala Trp Ala Leu Gly Asp Leu Arg Ala Lys Val Ala Phe Arg
340 345 350gag gag cct ccg ctg gat ctg ccg ccc gat gtg gcc gcg gac gtg ggc 1104Glu Glu Pro Pro Leu Asp Leu Pro Pro Asp Val Ala Ala Asp Val Gly
355 360 365gac agc gtg cgc aac ccg ccg agc ctc ctg gag tcc ctg cat ctg gac 1152Asp Ser Val Arg Asn Pro Pro Ser Leu Leu Glu Ser Leu His Leu Asp
370 375 380gcg cag agc gtg acc tgg atg ttc gtc gcg ctg ctg ctg ctc tcc ggc 1200Ala Gln Ser Val Thr Trp Met Phe Val Ala Leu Leu Leu Leu Ser Gly385 390 395 400ggg atc gcc ttc ctg atc ggg ctg ggc gag gag cat ccg tat ctc gcg 1248Gly Ile Ala Phe Leu Ile Gly Leu Gly Glu Glu His Pro Tyr Leu Ala
405 410 415gcg tac cgg acc acg gcc gag cgg ctg cgg gag ctg gag cgg gac atg 1296Ala Tyr Arg Thr Thr Ala Glu Arg Leu Arg Glu Leu Glu Arg Asp Met
420 425 430gag acg gat ctc gcg ggt tcc gag cgt gcc aag gag gcc gag gcc acc 1344Glu Thr Asp Leu Ala Gly Ser Glu Arg Ala Lys Glu Ala Glu Ala Thr
435 440 445ctg ggt gcc cgc gcg gag gcc cgc cgc gcg gcc cat gag gcg cgg ctc 1392Leu Gly Ala Arg Ala Glu Ala Arg Arg Ala Ala His Glu Ala Arg Leu
450 455 460tac gcg gtc gac gat ctc tac gaa gcc gcg gcc cac gcc tat ctg gac 1440Tyr Ala Val Asp Asp Leu Tyr Glu Ala Ala Ala His Ala Tyr Leu Asp465 470 475 480ggg gtg gcc atg gag tcc agc gat ccg gcg gtc acg gag gcc gcc atg 1488Gly Val Ala Met Glu Ser Ser Asp Pro Ala Val Thr Glu Ala Ala Met
485 490 495cgg ctg tcc aag cag tgg ccg ctg ctg ccg cgc 1521Arg Leu Ser Lys Gln Trp Pro Leu Leu Pro Arg
500 505<210>46<211>507<212>PRT<213>Streptomyces hygroscopicus<220><221>misc_feature<222>(16)<223>Xaa=Leu.<220><221>misc_feature<222>(144)<223>Xaa=Arg or Gln.<400>46Met Ser Thr Pro Pro Ile Ala Gly Asp Asp Gln Thr Pro Arg Arg Xaa1 5 10 15Arg Leu Arg Arg Arg Arg Ala Asp Ala Asp Arg Gly Arg Arg Gly Gly
20 25 30Arg Ala Ser Ala Arg Arg Phe Pro Asp Gly Ala Leu Pro Gln Pro Glu
35 40 45Pro Val Ala Ser Asp Val Ile Arg Ala Gly Asp Ser Thr Trp Leu Arg
50 55 60Asp Arg Ala Arg Lys His Gly Ala Ser Ala Ala Thr Arg Lys Val Phe65 70 75 80Asp Pro Trp Val Leu Ala Gly Pro Asp Arg Val Pro Tyr Phe Ala Glu
85 90 95Leu Ala Ser Leu Arg Asn Arg Val Lys His Arg Leu Ala Glu Glu His
100 105 110Ala Arg Ala Glu Glu Asp Gly Ala Leu Glu Ala Ser Arg Val Arg Ala
115 120 125Ala Ala Thr Ala Ala Gly Glu Arg Leu Glu Arg Ala Gly Gln Arg Xaa
130 135 140Val Val Leu Glu Arg Gln Gln Thr Val Thr Thr Ala Gln Leu Asp Arg145 150 155 160Leu Ala Arg Arg Ala Asp Arg Trp Gln Thr Phe Arg Asp Thr Val Arg
165 170 175Gly Gly Phe Glu Arg Arg Trp Leu Arg Ala Arg Met Pro Ala Asp Gly
180 185 190Ser Asp Gly Thr Asp Pro Gly Arg Gln Gly Ala Thr Arg Arg Glu Asp
195 200 205Glu Pro Glu Thr Thr Gly His Ala Ser Trp Gln Ala Val Ser Glu Pro
210 215 220Asp Pro Val Ala Glu Ala Asp Ala Ala Asp Arg Ala Leu Ser Thr Arg225 230 235 240Ala Ala Trp Glu Gly Ala Ala Ala Arg Pro Gly Met Pro Arg Trp Met
245 250 255Lys Leu Gly Val Leu Ala Ala Leu Val Val Val Glu Leu Pro Val Tyr
260 265 270Tyr Ser Val Phe Glu Asn Leu His Gly Val Gly Arg Phe Ala Asp Leu
275 280 285Leu Ser Tyr Ser Leu Met Val Ala Val Ala Val Ala Met Ile Leu Ala
290 295 300Pro His Ile Ala Gly Trp Ile Leu Arg Arg Arg Ser Ala Thr Gly Ala305 310 315 320Val Arg Leu Ser Ala Val Pro Ala Leu Ala Leu Leu Gly Val Trp Ala
325 330 335Tyr Gly Ala Trp Ala Leu Gly Asp Leu Arg Ala Lys Val Ala Phe Arg
340 345 350Glu Glu Pro Pro Leu Asp Leu Pro Pro Asp Val Ala Ala Asp Val Gly
355 360 365Asp Ser Val Arg Asn Pro Pro Ser Leu Leu Glu Ser Leu His Leu Asp
370 375 380Ala Gln Ser Val Thr Trp Met Phe Val Ala Leu Leu Leu Leu Ser Gly385 390 395 400Gly Ile Ala Phe Leu Ile Gly Leu Gly Glu Glu His Pro Tyr Leu Ala
405 410 415Ala Tyr Arg Thr Thr Ala Glu Arg Leu Arg Glu Leu Glu Arg Asp Met
420 425 430Glu Thr Asp Leu Ala Gly Ser Glu Arg Ala Lys Glu Ala Glu Ala Thr
435 440 445Leu Gly Ala Arg Ala Glu Ala Arg Arg Ala Ala His Glu Ala Arg Leu
450 455 460Tyr Ala Val Asp Asp Leu Tyr Glu Ala Ala Ala His Ala Tyr Leu Asp465 470 475 480Gly Val Ala Met Glu Ser Ser Asp Pro Ala Val Thr Glu Ala Ala Met
485 490 495Arg Leu Ser Lys Gln Trp Pro Leu Leu Pro Arg
500 505<210>47<211>1791<212>DNA<213>Streptomyces hygroscopicus<220><221>misc_feature<222>(671,1039,1088)<223>S=c or g<220><221>misc_feature<222>(771,774)<223>N=a or t or c or g<220><221>misc_feature<222>(775,778,781,794)<223>M=a or c<220><221>misc_feature<222>(977,1029,1105)<223>K=g or t<220><221>misc_feature<222>(983,1016,1034,1709)<223>R=a or g<400>47atg atc aaa gac gcc agg ccc ccg gaa ccg ttc cag tat gac ccg gcg 48Met Ile Lys Asp Ala Arg Pro Pro Glu Pro Phe Gln Tyr Asp Pro Ala1 5 10 15tca ggc atc tac gag ggc gtt ctc cgg ttg act tcc ggg cgt ttt cag 96Ser Gly Ile Tyr Glu Gly Val Leu Arg Leu Thr Ser Gly Arg Phe Gln
20 25 30gag cgg gcc cta tgg gga gca ttc ccg ggt acc acc tca ccg ata cgg 144Glu Arg Ala Leu Trp Gly Ala Phe Pro Gly Thr Thr Ser Pro Ile Arg
35 40 45tct gac aga gaa tcc aat cga cat cca cat cgg cat cga caa cgg cat 192Ser Asp Arg Glu Ser Asn Arg His Pro His Arg His Arg Gln Arg His
50 55 60cca cat cgg cgt cgg cta cat cgg ccg cga acc ctt tgc ggg aga aag 240Pro His Arg Arg Arg Leu His Arg Pro Arg Thr Leu Cys Gly Arg Lys65 70 75 80cgg aat acc act gtc cgc aat gcg gct gtc cgg tgc ggg aat ttc cgt 288Arg Asn Thr Thr Val Arg Asn Ala Ala Val Arg Cys Gly Asn Phe Arg
85 90 95acc ggg gaa atc ggg gga tcc att tcc atg atc aca tct gac agt gtc 336Thr Gly Glu Ile Gly Gly Ser Ile Ser Met Ile Thr Ser Asp Ser Val
100 105 110aac ggg gtg gtg cgc cgc ggc agg ctc ggc cgt acc gcc cgc ttc gcg 384Asn Gly Val Val Arg Arg Gly Arg Leu Gly Arg Thr Ala Arg Phe Ala
115 120 125gcc cgc tgg cgg ggc aag cgc gac ggc gcg cgc ggc gtc ccc cgc atc 432Ala Arg Trp Arg Gly Lys Arg Asp Gly Ala Arg Gly Val Pro Arg Ile
130 135 140gtg ctc ccg gag ccg tcc ggg gag cag cgg agc aag acg ccg ccg ccc 480Val Leu Pro Glu Pro Ser Gly Glu Gln Arg Ser Lys Thr Pro Pro Pro145 150 155 160atc gag cca ccc gct ccc gaa ctg ctg atc acc cct tac gtg atg gag 528Ile Glu Pro Pro Ala Pro Glu Leu Leu Ile Thr Pro Tyr Val Met Glu
165 170 175gtg cgg acc ggc gtc cgc cga acc acc gag cag atg cgc tcc gcc ctc 576Val Arg Thr Gly Val Arg Arg Thr Thr Glu Gln Met Arg Ser Ala Leu
180 185 190atc ggg cgg gag cac gcc ctg ctc agc agg ttg cgc gcc gag tcg gtg 624Ile Gly Arg Glu His Ala Leu Leu Ser Arg Leu Arg Ala Glu Ser Val
195 200 205cgc gtg gtc acc cag tac gac gtc cgc gag gac ccc cgg ccc gcg gsg 672Arg Val Val Thr Gln Tyr Asp Val Arg Glu Asp Pro Arg Pro Ala Xaa
210 215 220ctc gcg cgc tac ggc cac tgg gtg ggt cag tgg cgc acc agc gtg gac 720Leu Ala Arg Tyr Gly His Trp Val Gly Gln Trp Arg Thr Ser Val Asp225 230 235 240cgg tgc cga tcg cat gcc cat gcc gtg gtg gac cag gcc aat cag cga 768Arg Cys Arg Ser His Ala His Ala Val Val Asp Gln Ala Asn Gln Arg
245 250 255ctn ssn mtg mta mtg gga cgc ggt gmg cga gac cca ccc cca gct ctc 816Xaa Xaa Xaa Xaa Xaa Gly Arg Gly Xaa Arg Asp Pro Pro Pro Ala Leu
260 265 270ccg cct ccc ccg gcg ccc gcc cgg gga ctg gct gcc cgg ccg ggt gga 864Pro Pro Pro Pro Ala Pro Ala Arg Gly Leu Ala Ala Arg Pro Gly Gly
275 280 285gct gga ccg gtc ctg gta cca gcc cga cgt ctg gct gct ggc cga cga 912Ala Gly Pro Val Leu Val Pro Ala Arg Arg Leu Ala Ala Gly Arg Arg
290 295 300cga cag cac gcg gac ggc cac ctc ccg ggc gct gca cat act cga acg 960Arg Gln His Ala Asp Gly His Leu Pro Gly Ala Ala His Thr Arg Thr305 310 315 320gca gaa cac cga ccg ckt cga crg gag gac cgc gtg atg acc gtc cac 1008Ala Glu His Arg Pro Xaa Arg Xaa Glu Asp Arg Val Met Thr Val His
325 330 335act ccc gyt ccg gag cgc cck gcc gyc cgg sgg cac gga aac cgg cgg 1056Thr Pro Xaa Pro Glu Arg Xaa Ala Xaa Arg Xaa His Gly Asn Arg Arg
340 345 350cac gga aac cgg cgg cgc gcg ctg ccc gcc gsg ctg gtg ctc gct ctc 1104His Gly Asn Arg Arg Arg Ala Leu Pro Ala Xaa Leu Val Leu Ala Leu
355 360 365kcg gcg gcc gcc acg gcc tgc ggc tcc gac gag ccg tca cgc tac tcg 1152Xaa Ala Ala Ala Thr Ala Cys Gly Ser Asp Glu Pro Ser Arg Tyr Ser
370 375 380cag aca tgt ggt gtc gtg gtc gac ggc tcc ggc tcg gcc gac gcc tcc 1200Gln Thr Cys Gly Val Val Val Asp Gly Ser Gly Ser Ala Asp Ala Ser385 390 395 400cgg acc ggc ttc gac gcg gag gcc aag ctc aag gcc acc ctc cag acg 1248Arg Thr Gly Phe Asp Ala Glu Ala Lys Leu Lys Ala Thr Leu Gln Thr
405 410 415ttc ctg tcg gac aag aag tgc cgc aag acg tcc ttc gcc ccc ata acc 1296Phe Leu Ser Asp Lys Lys Cys Arg Lys Thr Ser Phe Ala Pro Ile Thr
420 425 430aag gtt tcc gag gcg tcg aag tgc cag gtc agc ccg ctc gac ctg gac 1344Lys Val Ser Glu Ala Ser Lys Cys Gln Val Ser Pro Leu Asp Leu Asp
435 440 445ccg gac acc tcg aag acc gcc gac cgc gag cgg acc cgc acc gcc atg 1392Pro Asp Thr Ser Lys Thr Ala Asp Arg Glu Arg Thr Arg Thr Ala Met
450 455 460cgt gcc gtc gcc ctc tcc aac gcc ctg aag ctg ctg cgc tgc gcc cag 1440Arg Ala Val Ala Leu Ser Asn Ala Leu Lys Leu Leu Arg Cys Ala Gln465 470 475 480aag gag gag ccc ggc tcc gat gtg ctc ggc ggg ctg tcg cgc atc gcg 1488Lys Glu Glu Pro Gly Ser Asp Val Leu Gly Gly Leu Ser Arg Ile Ala
485 490 495ctg tcc aag ccg agc ggt gac gac gcg tcg ttc gac gtc ctg gtg gtc 1536Leu Ser Lys Pro Ser Gly Asp Asp Ala Ser Phe Asp Val Leu Val Val
500 505 510agc gac ttc gac cag ggc gac acc gac ttc cgg ctc ggg cgg cag gac 1584Ser Asp Phe Asp Gln Gly Asp Thr Asp Phe Arg Leu Gly Arg Gln Asp
515 520 525ctg tcc acc gcc acc agc cgc cgg acc gtc atc gac gac ttc ctc aag 1632Leu Ser Thr Ala Thr Ser Arg Arg Thr Val Ile Asp Asp Phe Leu Lys
530 535 540tcg cac ggc aaa ccg aag ctg tcc ggc gcc gat gtc tac ccg gtg ggc 1680Ser His Gly Lys Pro Lys Leu Ser Gly Ala Asp Val Tyr Pro Val Gly545 550 555 560tac ggc atg aag tac cac acc gac acc tyc cgg tac gag cag ttc aac 1728Tyr Gly Met Lys Tyr His Thr Asp Thr Xaa Arg Tyr Glu Gln Phe Asn
565 570 575gcc ttc tgg acg gag ctt ctg gag ggg agg gtc aag gca cat gtc aac 1776Ala Phe Trp Thr Glu Leu Leu Glu Gly Arg Val Lys Ala His Val Asn
580 585 590acc acc tat cgc cgg 1791Thr Thr Tyr Arg Arg
595<210>48<211>597<212>PRT<213>Streptomyces hygroscopicus<220><221>misc_feature<222>(224)<223>Xaa=Gly or Ala.<220><221>misc_feature<222>(257)<223>Xaa=Leu.<220><221> misc_feature<222>(258)<223>Xaa=Gly or Ala or Arg or Pro.<220><221>misc_feature<222>(259)<223>Xaa=Met or Leu.<220><221>misc_feature<222>(260)<223>Xaa=Ile or Leu.<220><221>misc_feature<222>(261)<223>Xaa=Met or Leu.<220><221>misc_feature<222>(265)<223>Xaa=Glu or Ala.<220><221>misc feature<222>(326)<223>Xaa=Arg or Leu.<220><221>misc_feature<222>(328)<223>Xaa=Arg or Gln.<220><221>misc_feature<222>(339)<223>Xaa=Ala or Val.<220><221>misc_feature<222>(343)<223>Xaa=Pro.<220><221>misc_feature<222>(345)<223>Xaa=Ala or Val.<220><221>misc_feature<222>(347)<223>Xaa=Gly or Arg.<220><221>misc_feature<222>(363)<223>Xaa=Gly or Ala.<220><221>misc_feature<222>(369)<223>Xaa=Ala or Ser.<220><221>misc_feature<222>(570)<223>Xaa=Ser or Phe.<400>48Met Ile Lys Asp Ala Arg Pro Pro Glu Pro Phe Gln Tyr Asp Pro Ala1 5 10 15Ser Gly Ile Tyr Glu Gly Val Leu Arg Leu Thr Ser Gly Arg Phe Gln
20 25 30Glu Arg Ala Leu Trp Gly Ala Phe Pro Gly Thr Thr Ser Pro Ile Arg
35 40 45Ser Asp Arg Glu Ser Asn Arg His Pro His Arg His Arg Gln Arg His
50 55 60Pro His Arg Arg Arg Leu His Arg Pro Arg Thr Leu Cys Gly Arg Lys65 70 75 80Arg Asn Thr Thr Val Arg Asn Ala Ala Val Arg Cys Gly Asn Phe Arg
85 90 95Thr Gly Glu Ile Gly Gly Ser Ile Ser Met Ile Thr Ser Asp Ser Val
100 105 110Asn Gly Val Val Arg Arg Gly Arg Leu Gly Arg Thr Ala Arg Phe Ala
115 120 125Ala Arg Trp Arg Gly Lys Arg Asp Gly Ala Arg Gly Val Pro Arg Ile
130 135 140Val Leu Pro Glu Pro Ser Gly Glu Gln Arg Ser Lys Thr Pro Pro Pro145 150 155 160Ile Glu Pro Pro Ala Pro Glu Leu Leu Ile Thr Pro Tyr Val Met Glu
165 170 175Val Arg Thr Gly Val Arg Arg Thr Thr Glu Gln Met Arg Ser Ala Leu
180 185 190Ile Gly Arg Glu His Ala Leu Leu Ser Arg Leu Arg Ala Glu Ser Val
195 200 205Arg Val Val Thr Gln Tyr Asp Val Arg Glu Asp Pro Arg Pro Ala Xaa
210 215 220Leu Ala Arg Tyr Gly His Trp Val Gly Gln Trp Arg Thr Ser Val Asp225 230 235 240Arg Cys Arg Ser His Ala His Ala Val Val Asp Gln Ala Asn Gln Arg
245 250 255Xaa Xaa Xaa Xaa Xaa Gly Arg Gly Xaa Arg Asp Pro Pro Pro Ala Leu
260 265 270Pro Pro Pro Pro Ala Pro Ala Arg Gly Leu Ala Ala Arg Pro Gly Gly
275 280 285Ala Gly Pro Val Leu Val Pro Ala Arg Arg Leu Ala Ala Gly Arg Arg
290 295 300Arg Gln His Ala Asp Gly His Leu Pro Gly Ala Ala His Thr Arg Thr305 310 315 320Ala Glu His Arg Pro Xaa Arg Xaa Glu Asp Arg Val Met Thr Val His
325 330 335Thr Pro Xaa Pro Glu Arg Xaa Ala Xaa Arg Xaa His Gly Asn Arg Arg
340 345 350His Gly Asn Arg Arg Arg Ala Leu Pro Ala Xaa Leu Val Leu Ala Leu
355 360 365Xaa Ala Ala Ala Thr Ala Cys Gly Ser Asp Glu Pro Ser Arg Tyr Ser
370 375 380Gln Thr Cys Gly Val Val Val Asp Gly Ser Gly Ser Ala Asp Ala Ser385 390 395 400Arg Thr Gly Phe Asp Ala Glu Ala Lys Leu Lys Ala Thr Leu Gln Thr
405 410 415Phe Leu Ser Asp Lys Lys Cys Arg Lys Thr Ser Phe Ala Pro Ile Thr
420 425 430Lys Val Ser Glu Ala Ser Lys Cys Gln Val Ser Pro Leu Asp Leu Asp
435 440 445Pro Asp Thr Ser Lys Thr Ala Asp Arg Glu Arg Thr Arg Thr Ala Met
450 455 460Arg Ala Val Ala Leu Ser Asn Ala Leu Lys Leu Leu Arg Cys Ala Gln465 470 475 480Lys Glu Glu Pro Gly Ser Asp Val Leu Gly Gly Leu Ser Arg Ile Ala
485 490 495Leu Ser Lys Pro Ser Gly Asp Asp Ala Ser Phe Asp Val Leu Val Val
500 505 510Ser Asp Phe Asp Gln Gly Asp Thr Asp Phe Arg Leu Gly Arg Gln Asp
515 520 525Leu Ser Thr Ala Thr Ser Arg Arg Thr Val Ile Asp Asp Phe Leu Lys
530 535 540Ser His Gly Lys Pro Lys Leu Ser Gly Ala Asp Val Tyr Pro Val Gly545 550 555 560Tyr Gly Met Lys Tyr His Thr Asp Thr Xaa Arg Tyr Glu Gln Phe Asn
565 570 575Ala Phe Trp Thr Glu Leu Leu Glu Gly Arg Val Lys Ala His Val Asn
580 585 590Thr Thr Tyr Arg Arg
595<210>49<211>705<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1)..(705)<223><220><221>misc_feature<222>(556,561)<223>R=a or g<220><221>misc-feature<222>(557)..(558)<223>M=a or c<220><221>misc_feature<222>(590)<223>W=a or t<400>49gtg gag aac gtc cca gag cgc gca gag ccc acg ctc cgg atc agt cag 48Val Glu Asn Val Pro Glu Arg Ala Glu Pro Thr Leu Arg Ile Ser Gln1 5 10 15aca cac ttc ccg gtg gag acc ctg ggt ccc ggg cgg cgc ctg gcc gtc 96Thr His Phe Pro Val Glu Thr Leu Gly Pro Gly Arg Arg Leu Ala Val
20 25 30tgg tcg cag ggg tgc gga ctg gcc tgc gcc ggc tgt atg tcc cgg cac 144Trp Ser Gln Gly Cys Gly Leu Ala Cys Ala Gly Cys Met Ser Arg His
35 40 45acc tgg gat ccg cga ggc ggc gcc tct cgt acg gtg tcg tcc ctg ctc 192Thr Trp Asp Pro Arg Gly Gly Ala Ser Arg Thr Val Ser Ser Leu Leu
50 55 60ggg ctg tgg cgc gag gcg ttg gcg cgc ggc gcg gac ggg ctg acg atc 240Gly Leu Trp Arg Glu Ala Leu Ala Arg Gly Ala Asp Gly Leu Thr Ile65 70 75 80agc ggc ggg gag ccg ctc gac cag ccc gcc gct ctg gag gcc ctg ctg 288Ser Gly Gly Glu Pro Leu Asp Gln Pro Ala Ala Leu Glu Ala Leu Leu
85 90 95gcc ggg gcg gtg cgg gcc cgt gcg gag gcg gtg gca tcg ggc ggc ccg 336Ala Gly Ala Val Arg Ala Arg Ala Glu Ala Val Ala Ser Gly Gly Pro
100 105 110gcg gcg ggc cgt gag atc gac atc ctc ctc tac acg ggg tac gag gag 384Ala Ala Gly Arg Glu Ile Asp Ile Leu Leu Tyr Thr Gly Tyr Glu Glu
115 120 125gac gaa gtg gag cgt gac gcg gcg cgc tcc gcc gcc gtc cgc cac gcc 432Asp Glu Val Glu Arg Asp Ala Ala Arg Ser Ala Ala Val Arg His Ala
130 135 140gat gcg ctg gtg acc gga cgc ttc cgg gtg gcc gag ccc acc gcg ctg 480Asp Ala Leu Val Thr Gly Arg Phe Arg Val Ala Glu Pro Thr Ala Leu145 150 155 160gtg tgg cgc ggc tcg gcg aac cag cgc ata cgg ccg cgt acg gcg cgc 528Val Trp Arg Gly Ser Ala Asn Gln Arg Ile Arg Pro Arg Thr Ala Arg
165 170 175ggg tgg gcg cgc tac cag gag cat ctg rmm cgr acg gag agc ggg ccg 576Gly Trp Ala Arg Tyr Gln Glu His Leu Xaa Arg Thr Glu Ser Gly Pro
180 185 190cgt cta cag gtg gwc gag ggg gag ggc gat gtg cgg ctc tac gga gtg 624Arg Leu Gln Val Xaa Glu Gly Glu Gly Asp Val Arg Leu Tyr Gly Val
195 200 205ccg cgg cgc ggc gaa ctg gtc gag ctg gag cgt cgg ttg cgg cgg gcg 672Pro Arg Arg Gly Glu Leu Val Glu Leu Glu Arg Arg Leu Arg Arg Ala
210 215 220ggg atc gcc ctc acc ggt gcg agc tgg cgc ccc 705Gly Ile Ala Leu Thr Gly Ala Ser Trp Arg Pro225 230 235<210>50<211>235<212>PRT<213>Streptomyces hygroscopicus<220><221>misc_feature<222>(186)<223>Xaa=Glu or Asp or Ala or Lys or Asn or Thr.<220><221>misc_feature<222>(197)<223>Xaa=Asp or Val.<400>50Val Glu Asn Val Pro Glu Arg Ala Glu Pro Thr Leu Arg Ile Ser Gln1 5 10 15Thr His Phe Pro Val Glu Thr Leu Gly Pro Gly Arg Arg Leu Ala Val
20 25 30Trp Ser Gln Gly Cys Gly Leu Ala Cys Ala Gly Cys Met Ser Arg His
35 40 45Thr Trp Asp Pro Arg Gly Gly Ala Ser Arg Thr Val Ser Ser Leu Leu
50 55 60Gly Leu Trp Arg Glu Ala Leu Ala Arg Gly Ala Asp Gly Leu Thr Ile65 70 75 80Ser Gly Gly Glu Pro Leu Asp Gln Pro Ala Ala Leu Glu Ala Leu Leu
85 90 95Ala Gly Ala Val Arg Ala Arg Ala Glu Ala Val Ala Ser Gly Gly Pro
100 105 110Ala Ala Gly Arg Glu Ile Asp Ile Leu Leu Tyr Thr Gly Tyr Glu Glu
115 120 125Asp Glu Val Glu Arg Asp Ala Ala Arg Ser Ala Ala Val Arg His Ala
130 135 140Asp Ala Leu Val Thr Gly Arg Phe Arg Val Ala Glu Pro Thr Ala Leu145 150 155 160Val Trp Arg Gly Ser Ala Asn Gln Arg Ile Arg Pro Arg Thr Ala Arg
165 170 175Gly Trp Ala Arg Tyr Gln Glu His Leu Xaa Arg Thr Glu Ser Gly Pro
180 185 190Arg Leu Gln Val Xaa Glu Gly Glu Gly Asp Val Arg Leu Tyr Gly Val
195 200 205Pro Arg Arg Gly Glu Leu Val Glu Leu Glu Arg Arg Leu Arg Arg Ala
210 215 220Gly Ile Ala Leu Thr Gly Ala Ser Trp Arg Pro225 230 235<210>51<211>1218<212>DNA<213>Streptomyces hygroscopicus<220><221>CDS<222>(1)..(1218)<223><220><221>misc_feature<222>(118)<223>N=a or g or t or c<220><221>misc_feature<222>(257)<223>Y=c or t<220><221>misc_feature<222>(274)<223>S=c or g<400>51atg tgc ggc tct acg gag tgc cgc ggc gcg gcg aac tgg tcg agc tgg 48Met Cys Gly Ser Thr Glu Cys Arg Gly Ala Ala Asn Trp Ser Ser Trp1 5 10 15agc gtc ggt tgc ggc ggg cgg gga tcg ccc tca ccg gtg cga gct ggc 96Ser Val Gly Cys Gly Gly Arg Gly Ser Pro Ser Pro Val Arg Ala Gly
20 25 30gcc cct gag cgg ggg cgc ccg nct cgg gtg gca ggg cgc gcc gcg acc 144Ala Pro Glu Arg Gly Arg Pro Xaa Arg Val Ala Gly Arg Ala Ala Thr
35 40 45ccg gaa gcc gtg gcc tcg gcg gct gtg gtg ctt gcg gca gcg gac ctt 192Pro Glu Ala Val Ala Ser Ala Ala Val Val Leu Ala Ala Ala Asp Leu
50 55 60gtg gcg gtg gcc ttg gcg gct gtg gtc cct atg gct ggg tgc tcg gcg 240Val Ala Val Ala Leu Ala Ala Val Val Pro Met Ala Gly Cys Ser Ala65 70 75 80gcg atg gcc tcg gcg gyt gtg gtc ctt gcg gca sgg gcc gtt gtg gca 288Ala Met Ala Ser Ala Xaa Val Val Leu Ala Ala Xaa Ala Val Val Ala
85 90 95gag cgc cct gtg gcg gtg gtc tcg gcg gct gcg atc ctc tac gcc gga 336Glu Arg Pro Val Ala Val Val Ser Ala Ala Ala Ile Leu Tyr Ala Gly
100 105 110cgc atc gtg gcc ggc atc acc ggc gcc aca ggt gcg gtt gct ggc gcc 384Arg Ile Val Ala Gly Ile Thr Gly Ala Thr Gly Ala Val Ala Gly Ala
115 120 125tat atc gcc gac atc acc gat ggg gaa gat cgg gct cgc cac ttc ggg 432Tyr Ile Ala Asp Ile Thr Asp Gly Glu Asp Arg Ala Arg His Phe Gly
130 135 140ctc atg agc gct tgt ttc ggc gtg ggt atg gtg gca ggc ccc gtg gcc 480Leu Met Ser Ala Cys Phe Gly Val Gly Met Val Ala Gly Pro Val Ala145 150 155 160ggg gga ctg ttg ggc gcc atc tcc ttg cat gca cca ttc ctt gcg gcg 528Gly Gly Leu Leu Gly Ala Ile Ser Leu His Ala Pro Phe Leu Ala Ala
165 170 175gcg gtg ctc aac ggc ctc aac cta cta ctg ggc tgc ttc cta atg cag 576Ala Val Leu Asn Gly Leu Asn Leu Leu Leu Gly Cys Phe Leu Met Gln
180 185 190gag tcg cat aag gga gag cgt cga ccg atg ccc ttg aga gcc ttc aac 624Glu Ser His Lys Gly Glu Arg Arg Pro Met Pro Leu Arg Ala Phe Asn
195 200 205cca gtc agc tcc ttc cgg tgg gcg cgg ggc atg act atc gtc gcc gca 672Pro Val Ser Ser Phe Arg Trp Ala Arg Gly Met Thr Ile Val Ala Ala
210 215 220ctt atg act gtc ttc ttt atc atg caa ctc gta gga cag gtg ccg gca 720Leu Met Thr Val Phe Phe Ile Met Gln Leu Val Gly Gln Val Pro Ala225 230 235 240gcg ctc tgg gtc att ttc ggc gag gac cgc ttt cgc tgg agc gcg acg 768Ala Leu Trp Val Ile Phe Gly Glu Asp Arg Phe Arg Trp Ser Ala Thr
245 250 255atg atc ggc ctg tcg ctt gcg gta ttc gga atc ttg cac gcc ctc gct 816Met Ile Gly Leu Ser Leu Ala Val Phe Gly Ile Leu His Ala Leu Ala
260 265 270caa gcc ttc gtc act ggt ccc gcc acc aaa cgt ttc ggc gag aag cag 864Gln Ala Phe Val Thr Gly Pro Ala Thr Lys Arg Phe Gly Glu Lys Gln
275 280 285gcc att atc gcc ggc atg gcg gcc gac gcg ctg ggc tac gtc ttg ctg 912Ala Ile Ile Ala Gly Met Ala Ala Asp Ala Leu Gly Tyr Val Leu Leu
290 295 300gcg ttc gcg acg cga ggc tgg atg gcc ttc ccc att atg att ctt ctc 960Ala Phe Ala Thr Arg Gly Trp Met Ala Phe Pro Ile Met Ile Leu Leu305 310 315 320gct tcc ggc ggc atc ggg atg ccc gcg ttg cag gcc atg ctg tcc agg 1008Ala Ser Gly Gly Ile Gly Met Pro Ala Leu Gln Ala Met Leu Ser Arg
325 330 335cag gta gat gac gac cat cag gga cag ctt caa gga tcg ctc gcg gct 1056Gln Val Asp Asp Asp His Gln Gly Gln Leu Gln Gly Ser Leu Ala Ala
340 345 350ctt acc agc cta act tcg atc att gga ccg ctg atc gtc acg gcg att 1104Leu Thr Ser Leu Thr Ser Ile Ile Gly Pro Leu Ile Val Thr Ala Ile
355 360 365tat gcc gcc tcg gcg agc aca tgg aac ggg ttg gca tgg att gta ggc 1152Tyr Ala Ala Ser Ala Ser Thr Trp Asn Gly Leu Ala Trp Ile Val Gly
370 375 380gcc gcc cta tac ctt gtc tgc ctc ccc gcg ttg cgt cgc ggt gca tgg 1200Ala Ala Leu Tyr Leu Val Cys Leu Pro Ala Leu Arg Arg Gly Ala Trp385 390 395 400agc cgg gcc acc tcg acc 1218Ser Arg Ala Thr Ser Thr
405<210>52<211>406<212>PRT<213>Streptomyces hygroscopicus<220><221>misc_feature<222>(40)<223>Xaa=Thr or Ala or Pro or Ser.<220><221>misc_feature<222>(86)<223>Xaa=Ala or Val.<220><221>misc_feature<222>(92)<223>Xaa=Gly or Arg.<400>52Met Cys Gly Ser Thr Glu Cys Arg Gly Ala Ala Asn Trp Ser Ser Trp1 5 10 15Ser Val Gly Cys Gly Gly Arg Gly Ser Pro Ser Pro Val Arg Ala Gly
20 25 30Ala Pro Glu Arg Gly Arg Pro Xaa Arg Val Ala Gly Arg Ala Ala Thr
35 40 45Pro Glu Ala Val Ala Ser Ala Ala Val Val Leu Ala Ala Ala Asp Leu
50 55 60Val Ala Val Ala Leu Ala Ala Val Val Pro Met Ala Gly Cys Ser Ala65 70 75 80Ala Met Ala Ser Ala Xaa Val Val Leu Ala Ala Xaa Ala Val Val Ala
85 90 95Glu Arg Pro Val Ala Val Val Ser Ala Ala Ala Ile Leu Tyr Ala Gly
100 105 110Arg Ile Val Ala Gly Ile Thr Gly Ala Thr Gly Ala Val Ala Gly Ala
115 120 125Tyr Ile Ala Asp Ile Thr Asp Gly Glu Asp Arg Ala Arg His Phe Gly
130 135 140Leu Met Ser Ala Cys Phe Gly Val Gly Met Val Ala Gly Pro Val Ala145 150 155 160Gly Gly Leu Leu Gly Ala Ile Ser Leu His Ala Pro Phe Leu Ala Ala
165 170 175Ala Val Leu Asn Gly Leu Asn Leu Leu Leu Gly Cys Phe Leu Met Gln
180 185 190Glu Ser His Lys Gly Glu Arg Arg Pro Met Pro Leu Arg Ala Phe Asn
195 200 205Pro Val Ser Ser Phe Arg Trp Ala Arg Gly Met Thr Ile Val Ala Ala
210 215 220Leu Met Thr Val Phe Phe Ile Met Gln Leu Val Gly Gln Val Pro Ala225 230 235 240Ala Leu Trp Val Ile Phe Gly Glu Asp Arg Phe Arg Trp Ser Ala Thr
245 250 255Met Ile Gly Leu Ser Leu Ala Val Phe Gly Ile Leu His Ala Leu Ala
260 265 270Gln Ala Phe Val Thr Gly Pro Ala Thr Lys Arg Phe Gly Glu Lys Gln
275 280 285Ala Ile Ile Ala Gly Met Ala Ala Asp Ala Leu Gly Tyr Val Leu Leu
290 295 300Ala Phe Ala Thr Arg Gly Trp Met Ala Phe Pro Ile Met Ile Leu Leu305 310 315 320Ala Ser Gly Gly Ile Gly Met Pro Ala Leu Gln Ala Met Leu Ser Arg
325 330 335Gln Val Asp Asp Asp His Gln Gly Gln Leu Gln Gly Ser Leu Ala Ala
340 345 350Leu Thr Ser Leu Thr Ser Ile Ile Gly Pro Leu Ile Val Thr Ala Ile
355 360 365Tyr Ala Ala Ser Ala Ser Thr Trp Asn Gly Leu Ala Trp Ile Val Gly
370 375 380Ala Ala Leu Tyr Leu Val Cys Leu Pro Ala Leu Arg Arg Gly Ala Trp385 390 395 400Ser Arg Ala Thr Ser Thr
405
Claims (3)
1st, the gene cluster of water-absorption rules biosynthesis geldanamycin, it is characterized in that the gene cluster is all responsible for the biosynthesis of geldanamycin, its composition includes:
1) gdnM, gene size is 819bp, encodes 273 amino acid;
2) gdnQ, gene size is 1233bp, encodes 411 amino acid;
3) gdnS, gene size is 630bp, encodes 210 amino acid;
4) gdnP, gene size is 993bp, encodes 331 amino acid;
5) gdnO, gene size is 1130bp, encodes 377 amino acid;
6) gdnA, gene size is 1152bp, encodes 384 amino acid;
7) gdnE, gene size is 444bp, encodes 148 amino acid;
8) gdnK, gene size is 882bp, encodes 294 amino acid;
9) gdnT, gene size is 774bp, encodes 258 amino acid;
10) dnB, gene size is 2115bp, encodes 705 amino acid;
11) gdnF, gene size is 1026bp, encodes 342 amino acid;
12) gdnG, gene size is 1224bp, encodes 408 amino acid;
13) gdnH, gene size is 1239bp, encodes 413 amino acid;
14) orf1, gene size is 678bp, encodes 226 amino acid;
15) orf2, gene size is 726bp, encodes 242 amino acid;
16) orf3, gene size is 504bp, encodes 168 amino acid;
17) orf4, gene size is 618bp, encodes 206 amino acid;
18) orf5, gene size is 2721bp, encodes 907 amino acid;
19) orf6, gene size is 969bp, encodes 323 amino acid;
20) gdnC, gene size is 1659bp, encodes 553 amino acid;
21) gdnD, gene size is 3339bp, encodes 1113 amino acid;
22) gdn1, gene size is 1479bp, encodes 493 amino acid;
23) gdn2, gene size is 1521bp, encodes 507 amino acid;
24) gdn3, gene size is 1791bp, encodes 597 amino acid;
25) gdn4, gene size is 705bp, encodes 235 amino acid;
26) gdn5, gene size is 1218bp, encodes 406 amino acid.
2nd, the cloning process of geldanamycin biological synthesis gene cluster, it is characterized in that designing primer using AHBA biosynthesis genes conserved sequence:
- the AGAGGATCCTTCGAGCRSGAGTTCGC-3 ' of sense primer 5 '
BamH1
- the GCAGGATCCGGAMCATSGCCATGTAG-3 ' of anti-sense primer 5 '
Using water-absorption rules genomic DNA as template, performing PCR reaction is entered under LATaq enzyme effects, 755bp products are obtained, as probe, carried out colony hybridization with 17997 gene libraries and molecule hybridizes, and large-scale DNA sequencing is carried out to positive colony.
3rd, application of the geldanamycin biological synthesis gene cluster sequence in geldanamycin fermentation yield and Optimizing Reconstruction its structure is improved.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104024272A (en) * | 2011-10-12 | 2014-09-03 | 赛诺菲 | Gene cluster for biosynthesis of griselimycin and methylgriselimycin |
CN106191156A (en) * | 2016-08-01 | 2016-12-07 | 上海交通大学 | The method improving Ge Erdeng element fermentation level |
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2002
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104024272A (en) * | 2011-10-12 | 2014-09-03 | 赛诺菲 | Gene cluster for biosynthesis of griselimycin and methylgriselimycin |
CN104024272B (en) * | 2011-10-12 | 2018-04-20 | 赛诺菲 | Gene cluster for the biosynthesis of griselimycin and methyl griselimycin |
CN106191156A (en) * | 2016-08-01 | 2016-12-07 | 上海交通大学 | The method improving Ge Erdeng element fermentation level |
CN106191156B (en) * | 2016-08-01 | 2019-05-07 | 上海交通大学 | The method for improving Ge Erdeng element fermentation level |
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