CN1387041A - Method and standard for testing quality of medical artemisia pigweed - Google Patents

Abstract

A quality standard for medical artemisia pig weed is disclosed. Its verifying method includes examining appearance by visual observation, measuring water content by fast driver, testing general flavone by ultraviolet spectrophotometry, measuring soluble plant polyose by acid hydrolyzing for 10 min and iodometry, and testing organoselenium, organozinc and heavy metals (Hg, Pb and As) by atomic absorption spectrametry. Its advantage is high correctness.

Description

Testing quality of medical artemisia pigweed method and standard

[technical field]

The present invention relates to testing quality of medical artemisia pigweed method and standard.

[background technology]

Lamb's-quarters wormwood artemisia Artemisia Selengeusis Turez, eats dual-purpose wild plant at a kind of medicine.The original producton location is lakeshore grass continent, lakeside, Poyang.At the medicinal history in existing 3000 of China, still method and the standard of not having at present check lamb's-quarters wormwood artemisia quality of medicinal material, contain lamb's-quarters wormwood artemisia flavones (5 in the lamb's-quarters wormwood artemisia, 3-dihydroxy-3,7,4-trimethyl flavones), the fragrant glucoside of sieversin, absinthin, different sandlwood glucoside, cloud, plant polyose (synanthrin), scoparone, ayapanin, 7, tens of kinds of effective components such as 8-methylenedioxy group-6-methoxy coumarin, organic selenium.From the lamb's-quarters wormwood artemisia, propose these physiologically active things, can be made into various medicaments and health functional food.The medicinal exploitation of lamb's-quarters wormwood artemisia, necessary quality determining method and standard of working out medicinal lamb's-quarters wormwood artemisia.

[summary of the invention]

The present invention just provides testing quality of medical artemisia pigweed method and standard.

The present invention is achieved like this, and medicinal lamb's-quarters wormwood artemisia raw material quality standard is:

Project	Outward appearance	Moisture content	General flavone	The solubility plant polyose	Organic selenium	Organic zinc	Heavy metal Hg, Pb, As
								Index	Aubergine stem, leaf, unrooted, no-sundries	≤17％	≥0.831％	??≥17％	≥0.0435 mg/kg	≥35 mg/kg	?≤3 ?mg/kg

Its method of inspection is: (1) outward appearance: adopt range estimation; (2) moisture content: measure with quick moisture drying instrument; (3) general flavone: adopt ultraviolet spectrophotometry; (4) solubility plant polyose: acid hydrolysis 10 minutes, survey the total reducing sugar iodometric determination; (5) organic selenium: atomic absorption spectrography (AAS); (6) organic zinc: atomic absorption spectrography (AAS); (7) heavy metal (Hg, Pb, As): atomic absorption spectrography (AAS).

The present invention can control the quality of medicinal lamb's-quarters wormwood artemisia raw material, satisfy to produce the needs of medicament and health functional food, general flavone, plant polyose, organic selenium etc. is chosen main effective component make to detect index, and is scientific and reasonable, accuracy is high.

[embodiment 1

The present invention is achieved like this, and medicinal lamb's-quarters wormwood artemisia raw material quality standard is:

Project	Outward appearance	Moisture content	General flavone	The solubility plant polyose	Organic selenium	Organic zinc	Heavy metal Hg, Pb, As
								Index	Aubergine stem, leaf, unrooted, no-sundries	≤17％	≥0.831％	≥17％??	≥0.0435 mg/kg	≥35 mg/kg	?≤3 ?mg/kg

Its method of inspection is: (1) outward appearance: adopt range estimation; (2) moisture content: measure with quick moisture drying instrument; (3) general flavone: adopt ultraviolet spectrophotometry; (4) solubility plant polyose: acid hydrolysis 10 minutes, use iodometric determination; (5) organic selenium: atomic absorption spectrography (AAS); (6) organic zinc: atomic absorption spectrography (AAS); (7) heavy metal (Hg, Pb, As): atomic absorption spectrography (AAS).

The method of inspection of effective component general flavone: 1, instrument: 751G type ultraviolet-visible photometer, photoelectric analytical balance, apparatus,Soxhlet's; 2, reagent: rutin standard items, methyl alcohol, ethanol, sodium nitrite, aluminum phosphate, NaOH are the AR level; 3, assay method:

The preparation precision of standard solution takes by weighing at the control substance of Rutin 100mg of 120 ℃ of drying under reduced pressure to constant weight, puts in the 50ml measuring bottle, adds methyl alcohol 30ml, puts that low-grade fever makes dissolving in the water-bath, puts coldly, adds methyl alcohol and is diluted to scale, shakes up.The accurate 10ml that draws puts in the 100ml volumetric flask, adds 50% ethanol dilution to scale, shakes up, and promptly gets (containing anhydrous rutin 0.2mg among every 1ml).

Accurate standard solution 1.0,2.0,3.0,4.0,5.0 and the 6.0ml of drawing of the preparation of typical curve puts respectively in the 25ml volumetric flask, respectively adds 50% ethanol 6ml, add 5% sodium nitrite solution 1ml, make mixing, placed 6 minutes, add 10% aluminum nitrate solution 1ml, shake up, placed hydro-oxidation sodium solution 10ml 6 minutes, add 50% ethanol again to scale, shake up, placed 15 minutes, do blank simultaneously with reagent corresponding.Measuring absorbance log with spectrophotometric method at the wavelength place of 500nm, is ordinate with the absorbance log, and concentration is horizontal ordinate, the drawing standard curve.

It is 4.0g that the assay method precision of sample takes by weighing weight, puts in the apparatus,Soxhlet's, adds methyl alcohol 80ml, and reflux is to extracting liquid colourless, and cooling is filtered in the 100ml measuring bottle, is diluted to scale with methyl alcohol, shakes up.The accurate 3ml that draws puts in the 25ml measuring bottle, adds 50% ethanol dilution to scale and does blank; The in addition accurate 3ml that draws puts in the 25ml measuring bottle, and the method under the sighting target directrix curve preparation is measured absorbance log from " adding 50% ethanol 6ml " in accordance with the law, reads the weight (mg) of rutin the need testing solution from typical curve, calculating, promptly.

Claims (1)

1, a kind of testing quality of medical artemisia pigweed method and standard, medicinal lamb's-quarters wormwood artemisia raw material quality standard is: Project Outward appearance Moisture content General flavone The solubility plant polyose Organic selenium Organic zinc Heavy metal Hg, Pb, As Index Aubergine stem, leaf, unrooted, no-sundries ≤17％ ≥0.831％ ≥17％ ≥0.0435 mg/kg ≥35 mg/kg ≤3 mg/kg Its method of inspection is: (1) outward appearance: adopt range estimation; (2) moisture content: measure with quick moisture drying instrument; (3) general flavone: adopt ultraviolet spectrophotometry; (4) solubility plant polyose: acid hydrolysis 10 minutes, use iodometric determination; (5) organic selenium: atomic absorption spectrography (AAS); (6) organic zinc: atomic absorption spectrography (AAS); (7) heavy metal (Hg, Pb, As): atomic absorption spectrography (AAS).