CN102297847A - Method for measuring total sugar content of chlortetracycline fermentation broth with refractometer - Google Patents
Method for measuring total sugar content of chlortetracycline fermentation broth with refractometer Download PDFInfo
- Publication number
- CN102297847A CN102297847A CN2011102153050A CN201110215305A CN102297847A CN 102297847 A CN102297847 A CN 102297847A CN 2011102153050 A CN2011102153050 A CN 2011102153050A CN 201110215305 A CN201110215305 A CN 201110215305A CN 102297847 A CN102297847 A CN 102297847A
- Authority
- CN
- China
- Prior art keywords
- sugar content
- refractometer
- measuring
- value
- data
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention relates to a method for measuring the total sugar content of a chlortetracycline fermentation broth with a refractometer, and the method is characterized by accurate measuring result and low cost. The method comprises the steps of: (1) collecting original data: measuring the sugar content data of a standard sample by iodimetry, with the sugar content of the sample at a gradient concentration of 0-17%; (2) inputting the data measured in step (1) into the refractometer; (3) dropping the sample with sugar content of 0-17% for measuring directly in a determination analysis pool so as to conduct measuring. The method of the invention has the advantages of accurate measuring result and fast detection speed. Meanwhile, after one time of standard data input, no input and detection through iodometry are needed once again during sugar content detection of the sample at a later stage, so that a lot of reagents can be saved. The method of iodometry is complicated for operation, while the adoption of a refractometer is simple in operation. The employment of a refractometer for detection reduces the labor intensity of technicists and improves the working efficiency.
Description
Technical field
The present invention relates to the chemical example detection range, particularly a kind of method of testing of measuring aureomycin fermentation liquor total sugar content with refractometer.
Background technology
Feed aureomycin fermentation liquor total reducing sugar detection method is continued to use traditional iodimetric titration for many years at present, and not only workload is big, and minute is long, can not in time provide accurately, analyze reliably data to production.And the cost of measuring is higher, adopts iodometric determination aureomycin fermentation liquor total reducing sugar, and it is measured reagent and costs an arm and a leg.Measuring the waste liquid that uses simultaneously also can pollute environment, and sometimes in order to reduce cost, often only measure and once just draw conclusion, measurement result error rate height, can not satisfy the assay determination requirement,, directly adopt refractometer to detect because the sugar content of aureomycin solution is lower, its measurement result also contains deviation, can not satisfy the assay determination requirement.
Summary of the invention
The object of the present invention is to provide a kind of measurement result accurate, the method for testing with refractometer mensuration aureomycin fermentation liquor total sugar content with low cost.
The objective of the invention is to realize in the following manner: it may further comprise the steps: (1) raw data acquisition: adopt the data of the sugar content of iodometric determination standard model, wherein the sugar content scope of standard model is sugar content gradient concentration 0～17% sample in the step (1); (2) data that record in the step (1) are inputed in the refractometer, the sugar content that (3) will need to measure is that 0～17% sample directly drops on the determination and analysis pond of refractometer and measures.
The present invention has the following advantages: it is accurate 1, to adopt the refractometer measurement to have testing result, the advantage that detection speed is fast, 2, behind normal data of input, the later stage test sample sugar content time, need not to import by iodimetric titration once more to detect once more, saved a large amount of reagent, 3, owing to the iodimetric titration complicated operation, and refractometer is simple to operate, adopts refractometer to detect, reduce technician's labour intensity, improved work efficiency.
Description of drawings
The canonical plotting of Fig. 1 for recording by iodimetric titration.
Embodiment
The present invention a kind ofly measures the method for testing of aureomycin fermentation liquor total sugar content with refractometer, it it may further comprise the steps:
(1) raw data acquisition: the data that adopt the sugar content of iodometric determination standard model, it is characterized in that: data are the sugar content of collected specimens and the typical curve that corresponding refractive index is set up, wherein the numerical value of sugar content and the refractive index corresponding with sugar content is gathered the 5-7 group at least, and the digital average of sugar content is distributed between 0～17%, wherein the sugar content of each standard all will be measured three times and draw refractive index, and typical curve is got the mean value of refractive index.
(2) data that record in the step (1) are inputed in the refractometer; Described data are typical curve, get in the typical curve refractive index of some groups of sugar contents and sugar content correspondence, it are inputed in the refractometer get final product, and wherein sugar content is as the Y value of typical curve, and corresponding refractive index is as the X value of typical curve.
The step that specifically data is inputed to refractometer is as follows: 1. open refractometer, push menu control key to screen and ranges occurs under initial interface, 2. push acknowledgement key, this moment, rccess code appearred in screen, and the input password is also pushed acknowledgement key; When 3. pushing menu control key to screen and User Range occurring, import the total reducing sugar value (Y value) and the corresponding nd value (refractive index, i.e. X value) of sample respectively, can obtain the User Defined curve; 4. restart refractometer.
(3) sugar content that will need to measure is that 0～17% sample directly drops on the determination and analysis pond of refractometer and measures, and before measuring, needs to adjust refractometer, and the data (being typical curve) of input are originally switched.
Describe below in conjunction with concrete case:
By iodimetric titration a plurality of parameters are tested, and the canonical plotting that records by iodimetric titration, referring to Figure of description Fig. 1; The total reducing sugar value (Y value) of the corresponding standard sample of obtaining by typical curve among Fig. 1 and corresponding nd value (refractive index, i.e. X value): as shown in table 1:
User Defined curve U SER makes record
Table 1
Mean value among Fig. 2 one hurdle is inputed in the refractometer, can guarantee that the measured sugared content error of sample of measuring sugar content 0～17% in future is not more than 1.5%.
Claims (5)
1. method of testing of measuring aureomycin fermentation liquor total sugar content with refractometer, it may further comprise the steps: (1) raw data acquisition: adopt the data of the sugar content of iodometric determination standard model, it is characterized in that: wherein the sugar content scope of standard model is sugar content gradient concentration 0 ~ 17% sample in the step (1); (2) data that record in the step (1) are inputed in the refractometer, the sugar content that (3) will need to measure is that 0 ~ 17% sample directly drops on the determination and analysis pond of refractometer and measures.
2. the method for testing of measuring aureomycin fermentation liquor total sugar content with refractometer according to claim 1, it is characterized in that: the data described in the step (1) are the sugar content of collected specimens and the typical curve that corresponding refractive index is set up, wherein the numerical value of sugar content and the refractive index corresponding with sugar content is gathered the 5-7 group at least, and the digital average of sugar content is distributed between 0 ~ 17%, wherein the sugar content of each standard all will be measured three times and draw refractive index, and typical curve is got the mean value of refractive index.
3. the method for testing of measuring aureomycin fermentation liquor total sugar content with refractometer according to claim 1, it is characterized in that: the data described in the step (2) are typical curve; get in the typical curve refractive index of some groups of sugar contents and sugar content correspondence; it is inputed in the refractometer get final product; wherein sugar content is as the Y value of typical curve, and corresponding refractive index is as the X value of typical curve.
4. the method for testing of measuring aureomycin fermentation liquor total sugar content with refractometer according to claim 3, it is characterized in that: the method for in the step (2) data being imported in the refractometer is as follows: 1. open refractometer, under initial interface, push menu control key to screen and ranges occurs, 2. push acknowledgement key, this moment, rccess code appearred in screen, and the input password is also pushed acknowledgement key; When 3. pushing menu control key to screen and User Range occurring, import the total reducing sugar value (Y value) and the corresponding nd value (refractive index, i.e. X value) of sample respectively, can obtain the User Defined curve; 4. restart refractometer.
5. the method for testing of measuring aureomycin fermentation liquor total sugar content with refractometer according to claim 1, it is characterized in that: the sugar content that will need to measure is that 0 ~ 17% sample directly drops on the determination and analysis pond of refractometer and measures, before measuring, need to adjust refractometer, the data of input are originally switched out.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110215305 CN102297847B (en) | 2011-07-29 | 2011-07-29 | Method for measuring total sugar content of chlortetracycline fermentation broth with refractometer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110215305 CN102297847B (en) | 2011-07-29 | 2011-07-29 | Method for measuring total sugar content of chlortetracycline fermentation broth with refractometer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102297847A true CN102297847A (en) | 2011-12-28 |
| CN102297847B CN102297847B (en) | 2013-03-20 |
Family
ID=45358413
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110215305 Active CN102297847B (en) | 2011-07-29 | 2011-07-29 | Method for measuring total sugar content of chlortetracycline fermentation broth with refractometer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102297847B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106770991A (en) * | 2017-03-28 | 2017-05-31 | 农业部环境保护科研监测所 | A kind of method for determining zymotic fluid sugar content and its application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0116746A2 (en) * | 1983-02-18 | 1984-08-29 | American Crystal Sugar Company | Apparatus and method for measuring refractive index of liquids |
| CN87212066U (en) * | 1987-08-21 | 1988-03-30 | 江苏省丹徒县分析仪器厂 | High-speed liquid density meter |
| EP0899557B1 (en) * | 1997-08-26 | 2002-01-02 | Matsushita Electric Industrial Co., Ltd. | Element and apparatus for attenuated total reflection measurement, and method for measuring specific component using the same |
| CN1387041A (en) * | 2002-05-17 | 2002-12-25 | 王群 | Method and standard for testing quality of medical artemisia pigweed |
-
2011
- 2011-07-29 CN CN 201110215305 patent/CN102297847B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0116746A2 (en) * | 1983-02-18 | 1984-08-29 | American Crystal Sugar Company | Apparatus and method for measuring refractive index of liquids |
| CN87212066U (en) * | 1987-08-21 | 1988-03-30 | 江苏省丹徒县分析仪器厂 | High-speed liquid density meter |
| EP0899557B1 (en) * | 1997-08-26 | 2002-01-02 | Matsushita Electric Industrial Co., Ltd. | Element and apparatus for attenuated total reflection measurement, and method for measuring specific component using the same |
| CN1387041A (en) * | 2002-05-17 | 2002-12-25 | 王群 | Method and standard for testing quality of medical artemisia pigweed |
Non-Patent Citations (5)
| Title |
|---|
| 常履宪: "糖量折光仪快速测定糖精中所掺白糖", 《中国公共卫生》 * |
| 汪燕祥: "折射法在测定水基液中有效浓度的应用", 《腐蚀与防护》 * |
| 霍光华等: "折光法监测水溶性糖效果的研究", 《南昌大学学报（理科版）》 * |
| 马志泓 等: "甜高粱茎秆汁液锤度与总糖含量间相关性的研究", 《沈阳农业大学学报》 * |
| 高芳 等: "8301多糖中总糖的含量测定", 《中兽医医药杂志》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106770991A (en) * | 2017-03-28 | 2017-05-31 | 农业部环境保护科研监测所 | A kind of method for determining zymotic fluid sugar content and its application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102297847B (en) | 2013-03-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN2795848Y (en) | Integrated pesticide residue detector for vegetable and fruit | |
| CN201476844U (en) | Capacitive liquid-level detecting device | |
| CN109374697B (en) | Batch calibration method for detection of electrochemical formaldehyde sensor | |
| CN106201802A (en) | The CPU internal interrupt response time of logic-based analyser and the measuring method of recovery time | |
| CN103027694A (en) | Device used for testing movement of animal under forced swimming state | |
| CN1448712A (en) | A potentiometric titration instrument | |
| CN201576058U (en) | Proportion electromagnet performance testing system | |
| CN102297847B (en) | Method for measuring total sugar content of chlortetracycline fermentation broth with refractometer | |
| CN103440425B (en) | A kind of method for building up of octane value regression model | |
| CN203148516U (en) | Trace reagent liquid level detecting device for immunohistochemical stainer | |
| CN105866542A (en) | High-accuracy sliding frequency measurement method | |
| CN202562619U (en) | Multi-point continuous on-line vibrating screen amplitude detector | |
| CN202939149U (en) | Multichannel pH testing device | |
| CN103712977B (en) | Lead-acid battery negative pole plate lead plaster component detection method | |
| CN101701879A (en) | Ratio electromagnet performance test system | |
| CN108344476A (en) | A kind of system integrating formula coal sample precision weighing system and method based on PC machine | |
| CN201255733Y (en) | Biodiesel raw material and biodiesel acid number automatic determinator | |
| CN112180056A (en) | Maotai-flavor liquor tracing method and system based on rare earth element detection | |
| CN203469983U (en) | Improvement structure of reaction kettle for producing plasticizer | |
| CN102253167B (en) | Hydrolysis process based water-soluble polysaccharide analysis method | |
| CN204330109U (en) | The efficient input device of chemical detection sample weighing data | |
| CN205280115U (en) | Pick up calibration system | |
| CN101409859A (en) | Method for measuring buzzer sensitivity | |
| CN202453337U (en) | Device for detecting pH value of reaction kettle for producing aqueous solution dyes | |
| CN103940865A (en) | Rapid detector for beta-lactam antibiotic residue |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |