CN1387038A - Method for radially loading chromatographic cake in column - Google Patents
Method for radially loading chromatographic cake in column Download PDFInfo
- Publication number
- CN1387038A CN1387038A CN 01115264 CN01115264A CN1387038A CN 1387038 A CN1387038 A CN 1387038A CN 01115264 CN01115264 CN 01115264 CN 01115264 A CN01115264 A CN 01115264A CN 1387038 A CN1387038 A CN 1387038A
- Authority
- CN
- China
- Prior art keywords
- chromatographic
- post
- chromatographic cake
- cake
- side opening
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
A radial charging method for charging chromatographic cake in column to separate or renaturating/purifying biologic macromoleculae includes assembling cylindrical cavity with cylindrical body, sieve plate, distributor and press plates, and filling the mixture of chromatographic filler and reagent into said cylindrical cavity from side hole. Its advantages are easy charging, high column effect and smooth surface of chromatographic medium.
Description
The present invention relates to the filling process technology of a kind of bioengineering downstream chromatographic cake, particularly a kind of radially loading post method that is used for biomacromolecule separation or biomacromolecule while renaturation and purifying chromatographic cake.
For the chromatographic column of preparation and type of production,, can obtain satisfied post and imitate at present with axial pressure and radially pressurization technology filling chromatograph packing material.But these two kinds of methods are only applicable to the filling of diameter greater than 20 μ m granular fillers, chromatograph packing material and post length for filling diameter 1-10 μ m granulometric range are less than or equal to 1 chromatographic cake, up to the present, the method that does not also have this chromatographic cake of comparatively ideal filling.If adopt traditional handicraft to load this chromatographic cake, often have many deficiencies: the traditional handicraft of filling chromatographic column is from axial turnover, generally is only applicable to the chromatographic column of post length＞10.This technology, very high to the column packing technique requirement, the loose uneven situation of loading that waits of tight import appears exporting in careless slightly being easy to, and dress post reappearance is relatively poor.In addition, having adorned post generally will place later on to spend the night and make " tense-lax " degree in its post tend towards stability and could use; This traditional column packing technique is not applicable to that the post length is smaller or equal to 1 chromatographic cake.Otherwise being very easy to occur the post center loads closely and the loose situation of post jamb filling; Usually after adorn post (for example φ 4.6mm chromatographic column), easily with blade with the filler entrance point, also be the entrance point surface filler strickling of moving phase.And for " chromatographic cake " of φ 20-1000mm, it is impossible doing like this, does not also have simple effective method to make so big filling surface smooth and smooth; Usually chromatographic column just need be adorned post again after bearing the high pressure of long duration.And adorning again before the post, the chromatographic column entrance point of repairing depression can prolong its serviceable life, when repairing the chromatographic column entrance point of depression usually, must the dismounting column cap, and take down sieve plate and could implement.For the pillar of minor diameter, less because of post sieve plate area, be easier to take off.And the area of " chromatographic cake " sieve plate generally is 1～100 times of common chromatographic column, and distributor and sieve plate are chimeric closely, and it is very dead to be pressed into the groove of cylinder, is difficult to take off.If take off by force, usually can destroy distributor; When adopting the high-pressure homogenization method axially to pack the granule medium into chromatographic cake, because the diameter of chromatographic cake is bigger, sealing is difficulty relatively, and necessary first leak test refills post.After installing chromatograph packing material, still there is the problem of leakage, brings great difficulty for the work of dress post; Need bigger homogenate jar and utility appliance for bigger chromatographic cake, increase production cost.
The purpose of this invention is to provide a kind of radially loading post method that is used for the chromatographic cake of biomacromolecule separation or while renaturation and purifying, can under middle normal pressure power, make full use of the granule filled column imitate high, capacity is big, the advantage of favorable reproducibility, overcome bulky grain chromatograph packing material post and imitate the deficiencies in the prior art such as low, can load chromatographic cake quickly and easily, its post is imitated reach the highest, and reduce dress post cost.
Realization of the present invention: 1-3 side opening is set on the cylinder of chromatographic cake, at first is that cylinder, sieve plate, distributor, pressing plate are assembled fastening formation cylinder chamber successively; Secondly for the chromatographic cake of diameter, directly chromatographic cake is loaded on the high-pressure homogenization packing column machine, the homogenate of chromatograph packing material and reagent formation is adorned post from the side opening on the cylinder according to general dress column method less than 50mm.For the chromatographic cake of diameter, earlier adorn post, and then it is loaded on the high pressure homogenizer adorns post, so that the medium filling is tightr according to the method for general high voltage homogenate dress post with suction method greater than 50mm.Side opening on the cylinder also is used to add chromatographic media---after chromatographic cake uses a period of time,, reach the purpose of eliminating dead volume by the method for adding chromatographic media from side opening because the loss of chromatographic media has increased the dead volume in the chromatographic cake.The method of adding chromatographic media is identical with the radially loading column method; Side opening on the cylinder also is used to wash chromatographic cake---after chromatographic cake uses a period of time, on distributor and sieve plate, gather some impurity and do not entered biomacromolecule material in the chromatographic cake, these accumulations can increase the pressure of chromatographic system on the one hand, on the other hand can contaminate subsequent sample to be separated, so, need the periodic flushing chromatographic cake.The method of flushing chromatographic cake be with side opening as import, as outlet, feeding damping fluid or water can wash with the import and export on two pressing plates of chromatographic cake; Side opening on the cylinder also is used to take out the inefficacy chromatographic media---after chromatographic cake uses a period of time, system pressure significantly raises, post is imitated and is become very low, need to change chromatographic media, at this moment can be with the import and export on two pressing plates of chromatographic cake as import, side opening feeds water and goes out the chromatographic media of inefficacy as outlet, loads chromatographic cake again with radially loading post method.
Advantage of the present invention and good effect: the dress post is convenient time saving and energy saving easily; Directly do not adorn post owing to need not open the pressing plate at two ends after the leak detection, can guarantee the chromatographic cake no leakage, guarantee the smooth of dielectric surface, help improving post and imitate; Do not need to open two end clamp plates, direct side opening from chromatographic cake is added chromatographic media, eliminates the dead volume in the chromatographic cake; Can advance the accumulation on solvent washing distributor and the sieve plate from the side opening on the chromatographic cake, reduce the pressure of chromatographic system and the pollution of follow-up sample to be separated; Can take out the inefficacy chromatographic media from the side opening on the chromatographic cake, adorn post with this method again; When loading bigger chromatographic cake, do not need bigger homogenate jar and other utility appliance, reduced production cost.
Claims (5)
1, a kind of radially loading post method of chromatographic cake, it is characterized in that: at first be that cylinder, sieve plate, distributor, pressing plate are assembled fastening formation post cavity successively, next is that the homogenate that chromatograph packing material and reagent form is added the post cavity by the arbitrary side opening of the 1--3 that is provided with on a cylinder side opening, and the reagent process sieve plate except that chromatograph packing material, distributor filtration mainly are the import and export discharges from the moving phase of vertical direction.
2, the radially loading post method of chromatographic cake according to claim 1 is characterized in that side opening and homogenate jar on the high-pressure homogenization packing column machine directly are connected to adorn post.
3, the radially loading post method of chromatographic cake according to claim 1, it is characterized in that the chromatographic cake of diameter less than 50mm, directly chromatographic cake is loaded on the high-pressure homogenization packing column machine, the chromatograph packing material homogenate is adorned post from the side opening on the cylinder according to general dress column method.
4, the radially loading post method of chromatographic cake according to claim 1 is characterized in that the chromatographic cake of diameter greater than 50mm, earlier adorns post with suction method, and then it is loaded on the high pressure homogenizer adorns post according to general high voltage homogenate dress post method.
5, the radially loading post method of chromatographic cake according to claim 1 is characterized in that adding chromatographic media, flushing chromatographic cake, changes the inefficacy chromatographic media and all operate by side opening.
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01115264 CN1387038A (en) | 2001-05-18 | 2001-05-18 | Method for radially loading chromatographic cake in column |
| US10/477,977 US7208085B2 (en) | 2001-05-18 | 2002-05-16 | Caky chromatographic column and the method for producing it and its applications |
| PCT/CN2002/000333 WO2002095390A1 (en) | 2001-05-18 | 2002-05-16 | A caky chromatographic column and the method for producing it and its applications |
| DE60228078T DE60228078D1 (en) | 2001-05-18 | 2002-05-16 | CAKE-CHROMATOGRAPHIC PILLARS, METHOD FOR THE PRODUCTION THEREOF AND THEIR APPLICATIONS |
| AT02732319T ATE403861T1 (en) | 2001-05-18 | 2002-05-16 | CAKE-SHAPED CHROMATOGRAPHY COLUMN, METHOD FOR THE PRODUCTION THEREOF AND ITS APPLICATIONS |
| EP02732319A EP1396721B1 (en) | 2001-05-18 | 2002-05-16 | A caky chromatographic column and the method for producing it and its applications |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01115264 CN1387038A (en) | 2001-05-18 | 2001-05-18 | Method for radially loading chromatographic cake in column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1387038A true CN1387038A (en) | 2002-12-25 |
Family
ID=4661839
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01115264 Pending CN1387038A (en) | 2001-05-18 | 2001-05-18 | Method for radially loading chromatographic cake in column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1387038A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104428663A (en) * | 2012-06-21 | 2015-03-18 | 通用电气健康护理生物科学股份公司 | An adaptor for a chromatography column |
| CN104548663A (en) * | 2013-10-21 | 2015-04-29 | 天津汉荣生物技术有限公司 | Flow-dividing baffle for homogenate tank for filling chromatographic column |
| CN108037228A (en) * | 2017-10-23 | 2018-05-15 | 苏州普瑞马斯科技有限公司 | A kind of chromatographic cake and its radially loading column method |
-
2001
- 2001-05-18 CN CN 01115264 patent/CN1387038A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104428663A (en) * | 2012-06-21 | 2015-03-18 | 通用电气健康护理生物科学股份公司 | An adaptor for a chromatography column |
| US10201768B2 (en) | 2012-06-21 | 2019-02-12 | Ge Healthcare Bio-Sciences Ab | Adaptor for a chromatography column |
| CN104548663A (en) * | 2013-10-21 | 2015-04-29 | 天津汉荣生物技术有限公司 | Flow-dividing baffle for homogenate tank for filling chromatographic column |
| CN108037228A (en) * | 2017-10-23 | 2018-05-15 | 苏州普瑞马斯科技有限公司 | A kind of chromatographic cake and its radially loading column method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US6190560B1 (en) | Slurry fill method and valve | |
| CN102814063A (en) | Pressure filter and pressure filtration method thereof | |
| CN108404474B (en) | Split type multi-cavity filter-pressing sludge dewatering method | |
| CN1387038A (en) | Method for radially loading chromatographic cake in column | |
| CN2815464Y (en) | Liquid-phase chromatographic fractionation column | |
| EP0257582A2 (en) | Column for preparative liquid chromatography | |
| WO2002095390A1 (en) | A caky chromatographic column and the method for producing it and its applications | |
| CN108585425B (en) | Split type filter-pressing sludge dewatering device | |
| CN113443807B (en) | High-pressure sealed filter plate unit structure and using method | |
| CN218917136U (en) | Hollow fiber separation membrane testing device | |
| CN206188620U (en) | High -pressure dehydrator | |
| CN201522480U (en) | Spherical high-performance liquid chromatogram preparation column | |
| CN106746446A (en) | A kind of pressing dehydrator high | |
| CN209012174U (en) | Servo electric cylinders formula purifies and separates system | |
| CN220828297U (en) | Leak protection oil sprayer | |
| CN206543798U (en) | A kind of metal bellows flat mouth rifle | |
| CN205350354U (en) | Liquid phase analytic system bottom seal structure | |
| CN109865316A (en) | A kind of liquid-phase chromatographic column of recent specifications | |
| CN205361684U (en) | Be applied to quick dismantlement closing device of disk centrifuge video disc | |
| CN2442257Y (en) | Seal clamp for plastic tube inner pressure test machine | |
| CN205965166U (en) | Sheet frame of pressure filter | |
| CN213137910U (en) | Solid-liquid separation equipment for preparing biogas anaerobic fermentation liquid from vinasse | |
| CN215653998U (en) | Novel parallel feeding type filter plate assembly and parallel filter chamber of corresponding filter press | |
| CN211706131U (en) | High-pressure diaphragm vacuum heating type filter press | |
| CN212718063U (en) | Pressure regulating device of air exhaust tire pressure gun |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |