CN109865316A - A kind of liquid-phase chromatographic column of recent specifications - Google Patents
A kind of liquid-phase chromatographic column of recent specifications Download PDFInfo
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- CN109865316A CN109865316A CN201910256011.9A CN201910256011A CN109865316A CN 109865316 A CN109865316 A CN 109865316A CN 201910256011 A CN201910256011 A CN 201910256011A CN 109865316 A CN109865316 A CN 109865316A
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- column tube
- liquid
- column
- pipe fitting
- phase chromatographic
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Abstract
The present invention provides a kind of liquid-phase chromatographic columns of recent specifications, including column tube, column tube connector and the chromatograph packing material being filled in column tube, the internal diameter of column tube is 40 millimeters, is provided with internal thread hole on the lateral wall of column tube rear and front end, the rear and front end portion of column tube is provided with stainless steel filter disc, closes filler；Column tube connector includes front pillar pipe fitting and rear pillar pipe fitting, and front pillar pipe fitting and rear pillar pipe fitting pass through nut respectively and the rear and front end of column tube is connected and fixed.Diameter of the present invention is that 40 millimeters of liquid-phase chromatographic column not only meets separation of primary 100 milligrams of the completion to 1 gram-grade other style product, while saving solvent consumption 50 or more percent.The liquid-phase chromatographic column prepares effect stability, operates convenient, save the cost, shortens the production time, environmental-friendly, has huge economic value and broad application prospect.
Description
Technical field
The invention belongs to liquid-phase chromatographic column technical fields, and in particular to a kind of liquid-phase chromatographic column of recent specifications.
Background technique
Highly effective liquid phase chromatographic system is one of method indispensable in modern scientific research, for compound separation and
Analysis.Performance liquid chromatographic column is the core element of highly effective liquid phase chromatographic system, with the continuous development of Technological research, efficient liquid
The sample complexity that phase chromatography is faced is being continuously increased, and separating effect requires to be continuously improved, in addition, environmentally friendly cost and reagent at
Under this dual-pressure, there is an urgent need to have the sample of targetedly chromatographic column processing different size.
The separating effect of liquid-phase chromatographic column depends primarily on the length of chromatograph packing material and chromatographic column, and the load of liquid-phase chromatographic column
Sample amount is substantially dependent upon the unit cross-sectional area of chromatographic column, the i.e. diameter of liquid-phase chromatographic column.Traditional experiment room rank prepares chromatography
The diameter specifications of column are 10 millimeters, 20 millimeters, 30 millimeters, and 50 millimeters, and diameter is that 30 millimeters of liquid-phase chromatographic columns below are suitble to
100 milligrams of samples below are handled, diameter is the sample that 50 millimeters of liquid-phase chromatographic column is suitble to 1-3 grams of processing, wherein 100 milligrams
Sample within the scope of to 1 gram in most cases can only select diameter to handle several times for 30 millimeters of liquid-phase chromatographic column, in this way
It can guarantee separating effect, but a disadvantage is that it is time-consuming and laborious, solvent is also wasted, bigger pressure is caused to environmental improvement.If
It uses diameter to carry out 100 milligrams of separation to the other sample size of 1 gram-grade for 50 millimeters of liquid-phase chromatographic column, will cause the diffusion of sample
Separating effect is influenced, waste solvent and sample loss are passed through.
As scientific research deepens continuously more and more diversified with demand, 100 milligrams to 1 gram of sample is increasingly becoming experiment
The conventional specification of room research processing, using the method for separating for several times be obviously unable to satisfy current efficient research and development demand and save at
This demand.In consideration of it, this invention uses diameter for 40 millimeters of liquid-phase chromatographic column for the first time, and complete the chromatography of plurality of specifications
The filling work of filler.
Summary of the invention
It is an object of the invention to: in view of the deficiency of the prior art, the internal diameter for providing a kind of recent specifications is
40 millimeters of liquid-phase chromatographic column meets 100 milligrams of demands to the separation preparation of 1 gram-grade other style product, and having filled up internal diameter is 40 millis
The vacancy of the liquid-phase chromatographic column of rice.
To achieve the goals above, the invention adopts the following technical scheme:
A kind of liquid-phase chromatographic column of recent specifications, including column tube, column tube connector and the chromatograph packing material being filled in the column tube,
The internal diameter of the column tube is 40 millimeters, is provided with internal thread hole on the lateral wall of the column tube rear and front end, before the column tube
Both ends are provided with stainless steel filter disc afterwards, close the filler；The column tube connector includes front pillar pipe fitting and rear pillar pipe fitting,
It is provided with inlet opening in the middle part of the front pillar pipe fitting, fluid hole, the inlet opening are provided in the middle part of the rear pillar pipe fitting
Liquid phase pipeline is formed between fluid hole, and the front pillar pipe fitting and rear pillar pipe fitting are separately positioned on the front and back of the column tube
Both ends are arranged at intervals with multiple corresponding with the internal thread hole outer in the same circumferential direction in periphery of the inlet opening, fluid hole
The rear and front end that tapped through hole, the front pillar pipe fitting and rear pillar pipe fitting pass through nut and the column tube respectively is connected and fixed.
Further, the wall thickness of the column tube is 4.75 millimeters, can tolerate the pressure of 1000bar, while guaranteeing entire column
Pipe is lighter, convenient for operation.
Further, the column tube is stainless steel tube.
Further, the chromatograph packing material is positive phase chromatography stuffing or reverse-phase chromatography filler, partial size in 5-10 micron range
Interior chromatograph packing material.
Further, column tube connector side is six smooth face cylinder structures, is not only convenient for operating, also effective protection
The safety of column cap and operator.
Further, the stainless steel filter disc is micron order stainless steel filter disc, prevents filler from leaking out, and has and is preferably applicable in
Property and stability.
In conclusion the present invention as the above scheme is adopted, has the advantages that
1, the liquid-phase chromatographic column that diameter of the present invention is 40 millimeters not only meets 100 milligrams of primary completion points to 1 gram-grade other style product
From, while saving solvent consumption 50 or more percent.The liquid-phase chromatographic column prepares effect stability, operates convenient, save the cost,
Shorten the production time, it is environmental-friendly, there is huge economic value and broad application prospect.
2, when liquid-phase chromatographic column of the present invention is separated for 100 milligrams to 1 gram-grade other style product, diffusion is small, peak width control
Good, solvent-oil ratio relative cost is low.
Detailed description of the invention
Fig. 1 is cross-sectional view of the invention.
Fig. 2 is the structural schematic diagram of column tube connector.
Part name and serial number in figure:
1- column tube, 2- front pillar pipe fitting, 3- rear pillar pipe fitting, 4- chromatograph packing material, 5- stainless steel filter disc, 6- inlet opening, 7- go out liquid
Hole, 8- internal thread hole, 9- external screw thread through-hole.
Specific embodiment
Below with reference to embodiment to a kind of liquid-phase chromatographic column of recent specifications of the present invention, it is described further.
Embodiment
As shown in Figure 1 and Figure 2, the liquid-phase chromatographic column of recent specifications of the present invention, including column tube 1, column tube connector and be filled in
Chromatograph packing material 4 in column tube, the internal diameter of column tube 1 are 40 millimeters, are provided with internal thread hole 8 on the lateral wall of 1 rear and front end of column tube,
The rear and front end portion of column tube 1 is provided with stainless steel filter disc 5, closes filler；Column tube connector includes that front pillar pipe fitting 2 and rear column tube connect
First 3, the middle part of front pillar pipe fitting 2 is provided with inlet opening 6, and the middle part of rear pillar pipe fitting 3 is provided with fluid hole 7, inlet opening 6 and goes out
Liquid phase pipeline is formed between fluid apertures 7, and front pillar pipe fitting 2 and rear pillar pipe fitting 3 are separately positioned on the rear and front end of column tube 1,
Inlet opening 6, fluid hole 7 the same circumferential direction in periphery be arranged at intervals with multiple external screw thread through-holes 9 corresponding with internal thread hole 8, it is preceding
Column tube connector 2 and rear pillar pipe fitting 3 are connected and fixed by the rear and front end of nut and column tube 1 respectively.
Wherein, inlet opening 7 and fluid hole 8 are applicable in 1/8 liquid phase pipeline and 1/16 liquid phase pipeline, adapt to it is different in flow rate and
Application demand；
Chromatograph packing material 4 is positive the chromatograph packing material of phase chromatography stuffing or reverse-phase chromatography filler, partial size in 5-10 micron range, presents
Excellent applicability；
Internal thread hole 8 and external screw thread through-hole 9 are 6, and fixed effect is good, effectively avoid unbalance stress and poorly sealed leakage
Problem；
Preferably, column tube connector side is six smooth face cylinder structures, is not only convenient for operating, also effective guard column head and operation
The safety of personnel；
Preferably, the wall thickness of column tube 1 is 4.75 millimeters, can tolerate the pressure of 1000bar, while guaranteeing that entire column tube is lighter
Just, convenient for operation；
Preferably, column tube 1 is stainless steel tube；
Preferably, stainless steel filter disc 5 is micron order stainless steel filter disc, prevents filler from leaking out, and has better applicability and stabilization
Property.
It is not limited to this.
Working principle:
The working principle of liquid-phase chromatographic column of the present invention is that the distribution coefficient difference based on compound between liquid phase and solid phase is realized
Separation.Mixture is dissolved in mobile phase and enters liquid-phase chromatographic column by inlet opening, and the filler in liquid-phase chromatographic column is to different material
Adsorption capacity it is different, the speed by chromatographic column will be different, and different compounds will be flowed out from the fluid hole of liquid-phase chromatographic column,
To realize the separation of compound, the quantity of solvent consumed under the chromatographic column constant flow rate of different-diameter is different, to different quality
Sample requirement is also different, and 40 millimeters of liquid-phase chromatographic columns of the invention efficiently solve the demand of 100 milligrams to 1000 milligrams preparation amounts,
With great scientific research value and economic value.
The advantages and positive effects of the present invention are: due to the adoption of the above technical scheme, it, can be effective under working condition
100 milligrams of reply separate to the other sample of 1 gram-grade, and sample diffusion effect is small, and peak width controls good, solvent-oil ratio relative cost
It is low；And has and prepare effect stability, structure is simple, and assembly and disassembly are convenient, convenient for being conducive to other parts to part replacement
Reuse, manufacturing cost and use cost are saved, the advantages that good seal performance.
The foregoing is merely the preferred embodiments of invention, are not intended to limit the invention, all in spirit of the invention
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (6)
1. a kind of liquid-phase chromatographic column of recent specifications is filled out including column tube, column tube connector and the chromatography being filled in the column tube
Material, it is characterised in that: the internal diameter of the column tube is 40 millimeters, is provided with internal screw thread on the lateral wall of the column tube rear and front end
The rear and front end portion in hole, the column tube is provided with stainless steel filter disc, closes the filler；The column tube connector includes that preceding column tube connects
Head and rear pillar pipe fitting are provided with inlet opening in the middle part of the front pillar pipe fitting, are provided with out in the middle part of the rear pillar pipe fitting
Fluid apertures forms liquid phase pipeline between the inlet opening and fluid hole, and the front pillar pipe fitting and rear pillar pipe fitting are respectively set
In the rear and front end of the column tube, the same circumferential direction in periphery of the inlet opening, fluid hole be arranged at intervals with it is multiple with it is described
Before the corresponding external screw thread through-hole of internal thread hole, the front pillar pipe fitting and rear pillar pipe fitting pass through nut and the column tube respectively
Both ends are connected and fixed afterwards.
2. a kind of liquid-phase chromatographic column of recent specifications according to claim 1, it is characterised in that: the wall thickness of the column tube is
4.75 millimeters, it can tolerate the pressure of 1000bar.
3. a kind of liquid-phase chromatographic column of recent specifications according to claim 1, it is characterised in that: the column tube is stainless steel
Column tube.
4. a kind of liquid-phase chromatographic column of recent specifications according to claim 1, it is characterised in that: the chromatograph packing material is positive
The chromatograph packing material of phase chromatography stuffing or reverse-phase chromatography filler, partial size in 5-10 micron range.
5. a kind of liquid-phase chromatographic column of recent specifications according to claim 1, it is characterised in that: column tube connector side
For six smooth face cylinder structures.
6. a kind of liquid-phase chromatographic column of recent specifications according to claim 1, it is characterised in that: the stainless steel filter disc is
Micron order stainless steel filter disc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910256011.9A CN109865316A (en) | 2019-04-01 | 2019-04-01 | A kind of liquid-phase chromatographic column of recent specifications |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910256011.9A CN109865316A (en) | 2019-04-01 | 2019-04-01 | A kind of liquid-phase chromatographic column of recent specifications |
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| CN109865316A true CN109865316A (en) | 2019-06-11 |
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| CN201910256011.9A Pending CN109865316A (en) | 2019-04-01 | 2019-04-01 | A kind of liquid-phase chromatographic column of recent specifications |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115569674A (en) * | 2022-09-29 | 2023-01-06 | 中国科学院地球环境研究所 | Ion exchange chromatographic column capable of being rapidly mounted and dismounted |
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|---|---|---|---|---|
| EP0228628A2 (en) * | 1986-01-08 | 1987-07-15 | Saphirwerk Industrieprodukte AG | Precision dosing pump for liquids, especially for use in HPLC technology |
| CN102093172A (en) * | 2010-12-13 | 2011-06-15 | 云南瑞升烟草技术（集团）有限公司 | Method and equipment for preparing peculiar smell-free high-purity dimethyl ether applied to treating tobacco products |
| CN103083943A (en) * | 2011-11-03 | 2013-05-08 | 辽宁科技大学 | Drainage-type preparation chromatographic column |
| CN205730459U (en) * | 2016-05-11 | 2016-11-30 | 中国农业科学院茶叶研究所 | A kind of cryosphere analysis apparatus being applicable to enzyme purification |
| CN106932516A (en) * | 2017-04-18 | 2017-07-07 | 蔡明红 | Original position ultrasound washing device and ultrasonic elution process based on SPE sampling column |
| CN209790944U (en) * | 2019-04-01 | 2019-12-17 | 上海易创分析仪器有限公司 | Liquid chromatographic column with brand new specification |
-
2019
- 2019-04-01 CN CN201910256011.9A patent/CN109865316A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0228628A2 (en) * | 1986-01-08 | 1987-07-15 | Saphirwerk Industrieprodukte AG | Precision dosing pump for liquids, especially for use in HPLC technology |
| CN102093172A (en) * | 2010-12-13 | 2011-06-15 | 云南瑞升烟草技术（集团）有限公司 | Method and equipment for preparing peculiar smell-free high-purity dimethyl ether applied to treating tobacco products |
| CN103083943A (en) * | 2011-11-03 | 2013-05-08 | 辽宁科技大学 | Drainage-type preparation chromatographic column |
| CN205730459U (en) * | 2016-05-11 | 2016-11-30 | 中国农业科学院茶叶研究所 | A kind of cryosphere analysis apparatus being applicable to enzyme purification |
| CN106932516A (en) * | 2017-04-18 | 2017-07-07 | 蔡明红 | Original position ultrasound washing device and ultrasonic elution process based on SPE sampling column |
| CN209790944U (en) * | 2019-04-01 | 2019-12-17 | 上海易创分析仪器有限公司 | Liquid chromatographic column with brand new specification |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115569674A (en) * | 2022-09-29 | 2023-01-06 | 中国科学院地球环境研究所 | Ion exchange chromatographic column capable of being rapidly mounted and dismounted |
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