CN1384066A - Biological reinforcer for treating domestic sewage and surface water - Google Patents
Biological reinforcer for treating domestic sewage and surface water Download PDFInfo
- Publication number
- CN1384066A CN1384066A CN 01113913 CN01113913A CN1384066A CN 1384066 A CN1384066 A CN 1384066A CN 01113913 CN01113913 CN 01113913 CN 01113913 A CN01113913 A CN 01113913A CN 1384066 A CN1384066 A CN 1384066A
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- bacillus
- domestic sewage
- surface water
- bacterial strain
- reinforcer
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Abstract
The present invention belongs to the field of waste water treatment and especially biological engineering bacteria composition for treating domestic sewage. The composition contains pseudomonads as main component, bacillus, parvobacteria and Alcaligenes. The present invention aims at providing one effective domestic sewage treating measure.
Description
The invention belongs to the improvement field of a kind of sewage, surface water, particularly relate to a kind of employing bioengineered strain removal or degraded sanitary sewage compositions for use.
Because Economic development in recent years, problem of environmental pollution is also more and more outstanding, take Liaohe River Basin, the tool statistics, 8 urban industry wastewater discharges in the basin are annual 9.37 hundred million tons, 6.42 hundred million tons of basin sanitary sewage quantity dischargeds, and quite a few unprocessed Liaohe River that just is discharged into, because a large amount of factory effluents and sanitary sewage have been admitted in the Liaohe River, it is quite serious that the Liaohe River is polluted.The super V class of river water quality.(pollute the most serious, lost most of functions of use), the Liaohe River has been identified as the whole nation and has polluted one of the most serious river, pollute in Huaihe River also is like this, river improvement through 3 years, Huaihe River water quality does not have real improvement, and the situation of surface water is directly connected to people health, is one of problem of current primary solution.
At problem set forth above, the invention provides a kind of bio-enhancer of handling sanitary sewage, its purpose is to solve the problem that existing processing sanitary sewage does not have aspect existence such as well effective control measures.
The present invention comes to be realized by the following technical programs:
A kind of bio-enhancer of handling sanitary sewage is characterized in that: each composition in the said composition and mutual proportionlity (with by volume) as follows thereof:
False monospore bacillus 4, genus bacillus 2, bacillus pumilis 1, Alcaligenes 1
Wherein account for main advantage with false monospore bacillus.
Advantage of the present invention and effect:
We have developed processing sanitary sewage, the extraordinary bio-enhancer of surface water effect at present, and it mainly is made up of microorganism strains, and main component is, false monospore bacillus 4, genus bacillus 2, bacillus pumilis 1, Alcaligenes 1 wherein accounts for main advantage with false monospore bacillus.Preparation is rendered to processed dress or basin, and at short notice, this bio-enhancer is with pollutant removal.
Below by embodiment the present invention is specifically described:
The screening of dominant strain and F-strain and domestication, be characteristics according to bacterial strain, we are formulated as different concns with pollutent, bacterium to be selected is inserted in the nutrient solution of pollutant load different concns from low to high, last device domestication is selected the bacterial strain that adaptive faculty is strong, removal efficient is high and is continued cultivation, domestication.And according to the characteristics of various microorganism belonging to genus and the cometabolism effect between the bacterial strain, make various microorganism belonging to genus in nutrient solution, occupy different ratios, continue to cultivate, tame, it cultivates the domestication condition: PH6.8～7.5, T20～30 ℃, 6～12 hours residence time, revolution 60～250 commentaries on classics/min, DO.3～6mg/L.
This method realizes as follows:
A, separation adaptability bacterial strain;
The nutrient solution of three concentration of preparation is in triangular flask from contaminated waste water, pollute in the nutrient solution that concentration of substrate is respectively 150,300,450mg/L, from contaminated water or mud, take small amount of sample in triangular flask, last shaking table is cultivated 24h, switching is incubated in the nutrient solution of another same concentration, repeatable operation one month, inducible strain produces the enzyme of a certain specific pollutant of degraded.
B, efficient, the special degradation property bacterial strain of screening;
Take the aseptic technique means, press the liquid diluting method,, select anti-pollution concentration height and divide pure the strong bacterial strain of contaminant removal capacity to pure bacterium with strains separation.
C, breeding strain;
Select the efficient bacterial strain of number strain (3～6 strain), adopt that selection by mutation, protoplastis merge, the plasmid transfering means, stimulate bacterial strain to change, bacterial strain continues to be respectively 150,300 in pollution concentration, shaking culture in the nutrient solution of 450mg/L, repeatedly for several times;
D, enlarged culturing are made finished product bacteria suspension or pulvis;
The top fermentation jar is cultivated, 30 ℃ of culture temperature, and incubation time 12～36 hours, pH value 6.8～7.4 makes strain growth to logarithmic phase, and bacterial strain quantity reaches 10
9Individual/as more than the ml, can be made into the suspension shape, but also low-temperature freeze drying to become pulvis;
E, be invested in the actual waste water system.
After breeding strain, enlarged culturing is made before finished product bacteria suspension or the pulvis, measures the degradability of efficient bacterial strain to pollutent, carries out best proportioning;
Measure the degradability of single bacterial strain to pollutent, hybrid bacterial strain is to the degradability of pollutent, select the bacterial strain that degradation capability is strong, growth is vigorous, strong to environment-adapting ability, the contaminant degradation clearance is reached more than 90%, carrying out from new proportioning, making it to bring into play the cometabolism effect between the bacterial strain.
By the above method that provides, can in handling sanitary sewage, surface water, be mixed with following composition:
False monospore bacillus 4, genus bacillus 2, bacillus pumilis 1, Alcaligenes 1
Wherein account for main advantage with false monospore bacillus.
Said composition can reach the ideal level to the processing power of administering in sanitary sewage, the surface water.
Claims (1)
1, a kind of bio-enhancer of handling sanitary sewage, surface water is characterized in that: each composition in the said composition and mutual proportionlity (with by volume) as follows thereof:
False monospore bacillus 4, genus bacillus 2, bacillus pumilis 1, Alcaligenes 1
Wherein account for main advantage with false monospore bacillus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01113913 CN1384066A (en) | 2001-04-28 | 2001-04-28 | Biological reinforcer for treating domestic sewage and surface water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01113913 CN1384066A (en) | 2001-04-28 | 2001-04-28 | Biological reinforcer for treating domestic sewage and surface water |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1384066A true CN1384066A (en) | 2002-12-11 |
Family
ID=4660610
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01113913 Pending CN1384066A (en) | 2001-04-28 | 2001-04-28 | Biological reinforcer for treating domestic sewage and surface water |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1384066A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101311131B (en) * | 2007-05-23 | 2010-06-23 | 中国科学院沈阳应用生态研究所 | Sewage handling method and uses thereof |
-
2001
- 2001-04-28 CN CN 01113913 patent/CN1384066A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101311131B (en) * | 2007-05-23 | 2010-06-23 | 中国科学院沈阳应用生态研究所 | Sewage handling method and uses thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |