CN1367144A - Microbial pool device for treating ammonia-containing waste water and its culture method - Google Patents
Microbial pool device for treating ammonia-containing waste water and its culture method Download PDFInfo
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- CN1367144A CN1367144A CN 01145161 CN01145161A CN1367144A CN 1367144 A CN1367144 A CN 1367144A CN 01145161 CN01145161 CN 01145161 CN 01145161 A CN01145161 A CN 01145161A CN 1367144 A CN1367144 A CN 1367144A
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Abstract
The present invention relates to an equipment for culturing microbial pool for treating ammonia-contained waste water and its naturalization and extensive culture method. The culture equipment is formed from biological reactor which is connected with pump and subsider, and is connected with air compressor by means of air flowmeter. The nitrobacteri wild strain is irradiated by UV lamp and treated by diethyl sulfate for 20 min., then extensively cultured in the culture equipment for microbial population. Said invention is simple in production process, its treatment capacity is large, and cost is low, and it has no secondary pollution.
Description
Technical field
The present invention relates to the method for the device of culturing micro-organisms flora and domestication thereof, enlarged culturing.
Background technology
At present disclosed method and the technology (as steam blow-off method, break point chlorination, absorption method etc.) that is used to handle the chemical fertilizer industry ammonia-containing water all exists shortcomings such as energy consumption is big, cost is high (cost for wastewater treatment per ton is greater than 0.4 yuan), complicated operation.
Summary of the invention
The object of the present invention is to provide a kind of cultivation to be used for the method for the device of the microorganism species that ammonia-containing water handles and domestication thereof, enlarged culturing, it is big that it has the Ammonia-Containing Wastewater Treatment processing power, simple to operate, characteristics such as non-secondary pollution.
The present invention realizes as follows.Cultivation of the present invention is used for the device of the microorganism species of ammonia-containing water processing, and it is by bio-reactor [1], pump [2], [3], [4], and settling tank [5], air compressor [6], air flowmeter [7] are formed.One end of bio-reactor [1] is connected with settling tank [5] with pump [4] by pump [3], and the other end of bio-reactor [1] is connected with air compressor [6] by air flowmeter [7], and the water-in of bio-reactor [1] is connected with pump [2].Bio-reactor [1] adopts the bubble tower bio-reactor.
The cultural method of microorganism species of the present invention, adopt the device of cultivating the microorganism species that is used for the ammonia-containing water processing, the concrete operations step is: the nitrobacteria wild-type strain is prepared into bacterial suspension, place the ultraviolet lamp of 15W to shine for 10 seconds down, be that the ethyl sulfate of 1% (V/V) was handled 20 minutes with concentration then, and the bacterial suspension that disposes placed on the solid medium cultivated 5 days, it is big to select a strain bacterium colony, the nitrobacteria mutant strain that the ammonia nitrogen degradation ability is strong, with the pump [2] in this device, pump [3] and pump [4] are closed, be cultured bacterial classification inoculation nutrient solution in the reactor [1] then with the ammonia-containing water, pH value in the control bio-reactor [1] is 5～9, temperature is at 15 ℃～40 ℃, with air compressor [6] bubbling air in bio-reactor [1], and by gas meter [7] control air flow size, ventilation is carried out enlarged culturing and domestication to bacterial classification continuously, regularly stop ventilation every day, after treating sludge settling, abandoning supernatant, add waste water again, continue to cultivate domestication.After after a while, mud coagulates wadding to be increased, and settling ratio reaches about 10-20%, water outlet NH
3-N is very low, and show that the domestication of mud finishes this moment, can open pump [2], pump [3] and pump [4] system is moved continuously.Waste water is handled the back through bio-reactor [1] and is flowed in the settling tank [5], by activity sludge reflux amount in pump [4] the control settling tank [5].Activity sludge reflux is arrived in the bio-reactor [1], and discharge remaining active sludge.
Description of drawings
Accompanying drawing is a device schema of the present invention:
The 1st, the bio-reactor of cultivation bacterial classification, 2,3,4 all is the water pump that is used for cultured continuously, the 5th, settling tank, 6 are used to the air compressor of microorganism oxygen supply, and 7 is gas meter.
Embodiment
Embodiment one:
The cultural method of microorganism species, adopt the device of cultivating the microorganism species that is used for the ammonia-containing water processing, the concrete operations step is: the nitrobacteria wild-type strain is prepared into bacterial suspension, place the ultraviolet lamp of 15W to shine for 10 seconds down, be that the ethyl sulfate of 1% (V/V) was handled 20 minutes with concentration then, and the bacterial suspension that disposes placed on the solid medium cultivated 5 days, it is big to select a strain bacterium colony, the nitrobacteria mutant strain that the ammonia nitrogen degradation ability is strong, with the pump [2] in this device, pump [3] and pump [4] are closed, then cultured bacterial classification inoculation in reactor [1], be nutrient solution with the ammonia-containing water, pH value in the control bio-reactor [1] is 5～9, temperature with air compressor [6] bubbling air in bio-reactor [1], and is passed through gas meter [7] control air flow size at 15 ℃～40 ℃, ventilation is carried out enlarged culturing and domestication to bacterial classification continuously, regularly stop every day the ventilation, treat sludge settling after, abandoning supernatant, add waste water again, continue to cultivate domestication.After after a while, mud coagulates wadding to be increased, and settling ratio reaches about 10-20%, water outlet NH
3-N is very low, and show that the domestication of mud finishes this moment, can open pump [2], pump [3] and pump [4] system is moved continuously.Waste water is handled the back through bio-reactor [1] and is flowed in the settling tank [5], by activity sludge reflux amount in pump [4] the control settling tank [5].Activity sludge reflux is arrived in the bio-reactor [1], and discharge remaining active sludge.With manual simulation's ammonia-containing water, the COD of its waste water is 1270ppm, NH
4-N is 552ppm, and PH 7.5～7.8 feeds ventilation continuously in the reactor that the bacterial classification of having tamed is housed, and making hydraulic detention time is 8 hours, is 31ppm through handling its water outlet COD, NH
3-N is 4ppm, and PH is 6.8～7.0, and the COD clearance is 97.6%, NH
4-N clearance is 99.3.
Embodiment two:
Implementation step is with example 1, and waste water is that fertilizer plant of Jinling Petrochemical Co. its flooded condition of ammonia-containing water is PH7.5～8.0, NH
4-N is 428ppm, and COD is that 968ppm water outlet PH is 7.0～7.2, NH
4-N is 99.5% for the 2ppm clearance, and COD is 96.1% for the 39ppm clearance.
Embodiment three:
Implementation step is with 1, and waste water is that Cangzhou fertilizer plant of Dahua Group its flooded condition of ammonia-containing water is PH7.6～8.1, NH
4-N is 508ppm, and COD is that 1109ppm water outlet PH is 7.1～7.3, NH
4-N is 99.3% for the 3.5ppm clearance, and COD is 96.6% for the 39ppm clearance.
Embodiment four:
Implementation step is with 1, and waste water is that its flooded condition of Shijiazhuang Chemical-Fertilizer Factory, Hebei prov.'s ammonia-containing water is PH7.4～7.9, NH
4-N is 532ppm, and COD is 1235ppm, and water outlet PH is 6.9～7.1, NH
4-N is 99.2% for the 4.3ppm clearance, and COD is 96.7% for the 41ppm clearance.
By the visible employing of above-mentioned application example biological inoculum Ammonia-Containing Wastewater Treatment of the present invention, production technique is simple, and processing power is big, and cost is low, does not have secondary pollution.
Claims (3)
1, a kind of cultivation is used for the device of the microorganism species of ammonia-containing water processing, it is made up of pump [3], pump [4] and settling tank [5], an end that it is characterized in that bio-reactor [1] is connected with settling tank [5] with pump [4] by pump [3], the other end of bio-reactor [1] is connected with air compressor [6] by air flowmeter [7], and the water-in of bio-reactor is connected with pump [2].
2, cultivation according to claim 1 is used for the device of the microorganism species of ammonia-containing water processing, it is characterized in that described bio-reactor [1] adopts the bubble tower bio-reactor.
3, a kind of cultivation is used for the method for the microorganism species of ammonia-containing water processing, it is characterized in that adopting the device of cultivating the microorganism species that is used for the ammonia-containing water processing, the concrete operations step is: the nitrobacteria wild-type strain is prepared into bacterial suspension, place the ultraviolet lamp of 15W to shine for 10 seconds down, be that the ethyl sulfate of 1% (V/V) was handled 20 minutes with concentration then, and the bacterial suspension that disposes placed on the solid medium cultivated 5 days, it is big to select a strain bacterium colony, the nitrobacteria mutant strain that the ammonia nitrogen degradation ability is strong, with the pump [2] in this device, pump [3] and pump [4] are closed, be cultured bacterial classification inoculation nutrient solution in the bio-reactor [1] then with the ammonia-containing water, pH value in the control bio-reactor [1] is 5～9, temperature is at 15～40 ℃, with air compressor [6] bubbling air in bio-reactor [1], and by gas meter [7] control air flow size, ventilation is carried out enlarged culturing and domestication to bacterial classification continuously, regularly stop ventilation every day, after treating sludge settling, abandoning supernatant, add waste water again, continue to cultivate domestication.After after a while, mud coagulates wadding to be increased, and settling ratio reaches about 10～20%, water outlet NH
3-N is very low, and show that the domestication of mud finishes this moment, can open pump [2], pump [3] and pump [4] system is moved continuously.Waste water is handled the back through bio-reactor [1] and is flowed in the settling tank [5], by dirt mud quantity of reflux in pump [4] the control settling tank [5].Activity sludge reflux is arrived in the bio-reactor [1], and discharge remaining active sludge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01145161 CN1367144A (en) | 2001-05-07 | 2001-12-31 | Microbial pool device for treating ammonia-containing waste water and its culture method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN01117994.5 | 2001-05-07 | ||
| CN01117994 | 2001-05-07 | ||
| CN 01145161 CN1367144A (en) | 2001-05-07 | 2001-12-31 | Microbial pool device for treating ammonia-containing waste water and its culture method |
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| CN1367144A true CN1367144A (en) | 2002-09-04 |
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| CN 01145161 Pending CN1367144A (en) | 2001-05-07 | 2001-12-31 | Microbial pool device for treating ammonia-containing waste water and its culture method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104193091A (en) * | 2009-06-22 | 2014-12-10 | 住友重机械工业株式会社 | Method For Treating Wastewater Containing Ammonia Nitrogen |
| CN104556411A (en) * | 2015-01-19 | 2015-04-29 | 汕头高新区晟泰环保生物技术有限公司 | Microbial flora mother liquor cultivation generator and microbial sewage treatment method thereof |
| CN106190825A (en) * | 2016-08-27 | 2016-12-07 | 浙江竟成环保科技有限公司 | Bacterial strain culture device for sewage disposal |
-
2001
- 2001-12-31 CN CN 01145161 patent/CN1367144A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104193091A (en) * | 2009-06-22 | 2014-12-10 | 住友重机械工业株式会社 | Method For Treating Wastewater Containing Ammonia Nitrogen |
| CN104556411A (en) * | 2015-01-19 | 2015-04-29 | 汕头高新区晟泰环保生物技术有限公司 | Microbial flora mother liquor cultivation generator and microbial sewage treatment method thereof |
| CN106190825A (en) * | 2016-08-27 | 2016-12-07 | 浙江竟成环保科技有限公司 | Bacterial strain culture device for sewage disposal |
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