CN1365614A - Technique for detoxicating white spot syndrome virus of prawn - Google Patents
Technique for detoxicating white spot syndrome virus of prawn Download PDFInfo
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- CN1365614A CN1365614A CN 01107747 CN01107747A CN1365614A CN 1365614 A CN1365614 A CN 1365614A CN 01107747 CN01107747 CN 01107747 CN 01107747 A CN01107747 A CN 01107747A CN 1365614 A CN1365614 A CN 1365614A
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- ovum
- prawn
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- white spot
- syndrome virus
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Abstract
A technique for detoxicating the white spot syndrome of prawn embryo includes such steps as collecting spawn, measuring density of spawn, killing virus with organic iodine as detoxicant with concentration at 5-10 ppm for 10-15 min, and flushing. Its advantages include high detoxicating effect (100%) and high and stable hatchability (more than 85%).
Description
The invention belongs to the disease control technology in prawn and the fry production process, is to a kind of detoxification technology method of white spot syndrome virus (WSSV) in the large-scale production of prawn seed.
Before the present invention makes, the report and the research of similar aspect are all arranged, as Japanese Tao Shan and husband's " several disinfectant Network Le マ ェ ヒ fertilized egg ぉ ょ び ノ ウ Block リ ウ ス To is to The Ru toxicity " and " the epiphytotics test of iodine disinfectant prevention prawn fulminant " (1996) in Shandong Province Marine Culture Institute Lee Tian Bao etc. both at home and abroad.They utilize iodine disinfectant (povidon iodine) to the contrast of the toxicity of prawn fertilized egg, nauplius, zoea, mysis and the young shrimp young with influence the hatchability of fertile eggs aspect and study, but only limit to experimental scale.At present, Shang Weijian is useful on the research contents of production-scale prawn embryo technique for detoxicating white spot syndrome virus.
The objective of the invention is to propose a kind of technology operation sequence simple, be applicable to large-scale production, utilize organic iodine to kill the technology of prawn embryo white spot virus, cut off this viral vertical transmission, virus-free high healthy prawn seed is provided.
The present invention finishes by following technology operation sequence: at first ovum is collected in the bigger container and also accompanies by little inflation with gas stone, flip the surface impurity and make ovum reach rational density, take out organic iodine solution with finite concentration with dropper according to the amount of ovum liquid.Slowly splashing into soup and stir in bucket makes drug effect even.Through after a while, ovum in the bucket is siphoned in the 150 purpose bolting silk mesh bags flushing 3-4 time in the container that is equipped with hatchery isothermal seawater again with flexible pipe.Then, the ovum after the flushing being put into the hatchery hatching cultivates.
Ovum density is 500-800 grain/milliliter;
Organic iodine solution is Iodophor, goes back available polyethylene Iodophors etc.;
With concentration is 5-101ppm (available iodine content);
Drug treating time (promptly from putting medicine to ovum being siphoned in the 150 purpose bolting silk mesh bags during this period of time) is 10-15 minute.
Be described in detail extensive seed of the present invention below by embodiment and produce embryo's detoxification technology:
White spot virus (WSSV) is not through reproductive cell and the vertical transmission to prawn ' s virus that constitutes, but the vertical transmission that constitutes through extraneous medium from parent.Therefore, carried out in Chinese prawn and japonicus seed produce in 2000 should virus detoxification production test and detection.
One carries out the pre-treatment work of the technology of the present invention, guarantees ovum quality
Ovum quality is the guaranteed conditions of embryo's detoxification treatment and seed rearing, and its quality has mainly referred to ovum rate, fertilization rate and developmental character synchronously, and what influence these factors mainly is that close shrimp is selected and daily management.General good ovum rate and fertilization rate require more than 70%-80%, and developmental character is consistent as far as possible synchronously, avoids the ovum embryo and the young to mix, and bring difficulty for embryo's detoxification treatment and seed rearing.
The specified operational procedure of two the technology of the present invention
At first ovum is collected in the bigger container, container 0.2 cubic metre of circular glass steel drum commonly used or Plastic Drum, and accompany by little inflation with gas stone are with the write by hand net surperficial impurity that flips.Afterwards, detect ovum density and whether reach 500-800 grain/milliliter.Amount according to ovum liquid is taken out organic iodine solution with available iodine content 5-10ppm concentration with dropper, slowly splashes into soup after gas stone is shifted out and stir to make drug effect even in bucket, picks up counting simultaneously.Through after a while, ovum in the bucket is siphoned in the 150 purpose bolting silk mesh bags with flexible pipe.Should be 10-15 minute from putting medicine during this period of time to ovum being siphoned in the 150 purpose bolting silk mesh bags.In the container that hatchery isothermal seawater is housed, wash 3-4 time again, note not with the ovum in the hand extruding mesh bag.Then, the ovum after the flushing being put into the hatchery hatching cultivates.
1. ovum is collected
1) acquisition time: close shrimp egg-laying time mainly concentrated between 3 of day mornings at zero point to the second.So the Best Times of collecting ovum is 5 o'clock to 7 o'clock morning.
2) collection density: be meant here ovum is put into the density of handling in the container after close shrimp lays eggs the workshop collection.Think that through test of many times optimum density is a 500-800 grain ovum/every milliliter in the production, cause ovum embryo anoxic easily in the too high operation of density and influence incubation rate that density is crossed the low workload that can increase detoxification treatment.
2. rational use of medicines technology
1) with concentration and time: we have carried out laboratory and production test (seeing Table 1) with organic iodine (Iodophor).
Table 1: organic disinfectant (Iodophor) is to the influence of the prawn ovum and the young
| Water temperature | Control group (egg hatching rate) | Experimental group | |||||
| Drug concentration | Processing time and egg hatching rate | ||||||
| ????5min | ?10min | ?15min | ?20min | ?25min | |||
| ????22.5℃ | ????83.0％ | ????5ppm ????10ppm | ????77.0％ ????77.8％ | 85.0％ 87.5％ | 87.7％ 83.3％ | 80.9％ 82.4％ | 77.6％ 85.2％ |
| ????23.0℃ | ????73.9％ | ????15ppm ????20ppm | ????56.7％ ????54.7％ | 55.6％ 61.3％ | 53.3％ 48.8％ | 50.0％ 55.6％ | 46.3％ 58.8％ |
| ????25.5℃ | ????55.6％ | ????25ppm ????30ppm | ????49.4％ ????68.5％ | 60.0％ 40.9％ | 46.7％ 40.0％ | 36.3％ 25.3％ | ???/ ???/ |
Test shows that drug concentration is 5-10ppm (available iodine content), and when administration time was 10-15 minute, the egg hatching rate reached 83.3%-87.7%.Learn a skill through using nucleic acid probe dot hybridization technology and histopathology, carried out following the tracks of detection to carrying virus status, its prawn young all detected less than virus to each stage of juvenile prawn, and test result shows that embryo's virus elimination rate reaches 100% (seeing Table 2).
Table 2: organic iodine is handled the testing result of each phase young virus
| Experimental group | Batch | Positive | Negative | Sun cloudy ratio (%) |
| ????3 | ????0 | ????3 | ????0 | |
| Control group | ????9 | ????5 | ????2 | ????55 |
In prawn seed-rearing field, horizontal town, field, Jimo City, Shandong Province the technology of the present invention is carried out production application in 2000, and produced virus-free healthy Chinese prawn seedling 3,000 ten thousand tails, japonicus seedling 3,000,000 tails altogether.Through the tracking of carrying virus is detected, embryo's virus elimination rate is 100%, the egg hatching rate can be stabilized in more than 85%.
The technology of the present invention possesses following characteristics:
1. to prawn embryo detoxicating white spot syndrome virus rate height, do not affect the incubation rate of ovum, the cultivation production work of seed can normally be carried out.
2. detoxification medicament sources is extensive, and toxicity is low. The technical operation program is simple, is fit to be applied to large-scale production.
Claims (5)
1. prawn embryo technique for detoxicating white spot syndrome virus, it is characterized in that its technology operation sequence is: at first ovum is collected in the bigger container and accompanies by little inflation, flip the surface impurity and make ovum reach rational density, amount according to ovum liquid is taken out organic iodine liquid with finite concentration with dropper, slowly splashing into soup and stir in bucket makes drug effect even, through after a while, with flexible pipe ovum in the bucket is siphoned in the 150 purpose bolting silk mesh bags, flushing 3-4 time in the container that is equipped with hatchery isothermal seawater again, then, the ovum after the flushing being put into the hatchery hatching cultivates.
2. a kind of prawn embryo technique for detoxicating white spot syndrome virus according to claim 1 is characterized in that the ovum density of collecting is 500-800 grain/milliliter.
3. a kind of prawn embryo technique for detoxicating white spot syndrome virus according to claim 1 is characterized in that organic iodine solution is Iodophor, also can be povidon iodine etc.
4. a kind of prawn embryo technique for detoxicating white spot syndrome virus according to claim 1 is characterized in that with concentration be 5-10ppm (available iodine content).
5. a kind of prawn embryo technique for detoxicating white spot syndrome virus according to claim 1 is characterized in that drug treating time (promptly from putting medicine to ovum being siphoned in the 150 purpose bolting silk mesh bags during this period of time) is 10-15 minute.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011077476A CN1186983C (en) | 2001-01-18 | 2001-01-18 | Technique for detoxicating white spot syndrome virus of prawn |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011077476A CN1186983C (en) | 2001-01-18 | 2001-01-18 | Technique for detoxicating white spot syndrome virus of prawn |
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| CN1365614A true CN1365614A (en) | 2002-08-28 |
| CN1186983C CN1186983C (en) | 2005-02-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNB011077476A Expired - Fee Related CN1186983C (en) | 2001-01-18 | 2001-01-18 | Technique for detoxicating white spot syndrome virus of prawn |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103503863A (en) * | 2012-06-25 | 2014-01-15 | 江苏天一健消毒剂有限公司 | Aquatic product dedicated hanging bag colloidal iodine disinfection drug |
| CN106719200A (en) * | 2016-12-15 | 2017-05-31 | 雷州市海威水产养殖有限公司 | A kind of Penaeus Vannmei nauplius method for optimizing |
-
2001
- 2001-01-18 CN CNB011077476A patent/CN1186983C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103503863A (en) * | 2012-06-25 | 2014-01-15 | 江苏天一健消毒剂有限公司 | Aquatic product dedicated hanging bag colloidal iodine disinfection drug |
| CN106719200A (en) * | 2016-12-15 | 2017-05-31 | 雷州市海威水产养殖有限公司 | A kind of Penaeus Vannmei nauplius method for optimizing |
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| Publication number | Publication date |
|---|---|
| CN1186983C (en) | 2005-02-02 |
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