CN110763816A - Flowing water type toxicity test device for native reference tested organisms - Google Patents
Flowing water type toxicity test device for native reference tested organisms Download PDFInfo
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- CN110763816A CN110763816A CN201911184090.3A CN201911184090A CN110763816A CN 110763816 A CN110763816 A CN 110763816A CN 201911184090 A CN201911184090 A CN 201911184090A CN 110763816 A CN110763816 A CN 110763816A
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Abstract
The invention discloses a running water type toxicity test device of a local standard tested organism, which comprises 3 units of a test water preparation unit, a running water type toxicity test unit and a toxicity effect monitoring unit, wherein the running water type toxicity test unit comprises a test system, a circulating system, a filtering system, an automatic sampling system, a water supplementing and automatic dosing system and an automatic feeding system, different water quality parameters and conditions can be set autonomously according to the difference of the toxicity test conditions and the difference of habits of the local standard tested organism, the running water type toxicity test of the local standard tested organism in China is met, the environment is not polluted, the establishment of the standard of the aquatic organism and the related work of the test of the standard tested organism can be accelerated by applying the device, the labor cost and the human error in the process are reduced, and the device has good scientific research value and application benefit.
Description
Technical Field
The invention relates to the technical field of aquatic organism benchmark tests and ecotoxicology, in particular to a flowing water type toxicity test device for local benchmark tested organisms.
Background
The flow-through toxicity test is a test for testing the toxic effect of a contaminant on aquatic organisms in a constantly flowing solution. Through moisturizing and automatic reagent feeding system, make test solution, contrast solution constantly flow into the test container that contains benchmark biological subject, the concentration of pollutant keeps stable in the whole testing process.
The work of making the reference of aquatic organisms is systematically developed in China, the ecotoxicological test of the local aquatic organisms is an important component for researching the reference of the aquatic organisms, however, a uniform running water type toxicity test device for the local reference tested organisms is not available at home and abroad, and the test conditions of the local reference tested organisms except fishes and the test flow required by the reference of the aquatic organisms cannot be met.
Specifically, the current domestic running water devices are generally directed at international standard test organisms for chemical tests, such as zebra fish and the like, are all directed at fishes, and are not directed at small animals such as daphnia magna and the like. With the development of water quality reference research systems in China, the test work of local reference tested organisms also needs to be developed in large quantity.
Therefore, the problem that needs to be solved by those skilled in the art is how to provide a running water type toxicity test device capable of performing tests on native fishes in our country, particularly on small animals.
Disclosure of Invention
In view of the above, the invention provides a flow type toxicity test device for small-sized reference test crustaceans such as medaka, pseudorasbora, Tang fish, crucian carp, pelteobagrus fulvidraco and the like, which satisfy local reference test organisms in China, and do not cause environmental pollution, and the device has good scientific research value and application benefit.
In order to achieve the purpose, the invention adopts the following technical scheme:
a flowing water type toxicity test device of an indigenous benchmark tested organism comprises:
the device comprises a test water preparation unit for providing test water with different water quality parameters, a running water type toxicity test unit and a toxicity effect monitoring unit for identifying the growth state of a standard tested organism;
the flow type toxicity test unit comprises a plurality of groups of toxicity test systems, wherein each group of toxicity test system comprises a test system for carrying out toxicity test, a filtering system for filtering test solution, a circulating system for adjusting the flow rate of the test solution, an automatic sampling system for sampling and monitoring chemical substances in the test solution, a water supplementing and automatic medicine adding system for adjusting the water quantity/concentration of the test solution, and an automatic feeding system for adding bait into the test system;
it should be noted that each group of toxicity test systems of the test system, the filtering system and the circulating system are respectively and independently arranged, and the water supplementing and automatic medicine adding system, the automatic sampling system and the automatic feeding system are jointly used by a plurality of groups of toxicity test systems, so that the normal operation of the test is realized, and the space is saved to the greatest extent.
The input end of the test system is connected with the test water preparation unit;
the filtering system and the circulating system are both positioned below the testing system, and the testing system is respectively communicated with the filtering system and the circulating system;
the automatic sampling system is positioned at one side of the test system, samples the test solution in the test system through a mechanical arm within a fixed time, and is electrically connected with the water supplementing and automatic dosing system;
the water supplementing and automatic dosing system is positioned on one side of the test system, the water level of the test solution in the test system is detected through a liquid level meter, and the test water preparation unit and the circulating system are controlled to supplement water through a detection result;
the automatic feeding system is electrically connected with the circulating system;
the toxicity effect monitoring unit is used for performing infrared behavior scanning on the test system, and is electrically connected with the automatic sampling system.
Preferably, in the above-mentioned flowing water type toxicity test apparatus for the local standard test organism, the test water preparation unit adjusts water quality parameters of the test water including, but not limited to, water hardness, pH, temperature, and dissolved organic carbon DOC.
The beneficial effects of the above technical scheme are: the test water preparation unit is used for preparing test water, provides a test solution for a subsequent running water type toxicity test, and does not contain any substance toxic to tested organisms.
Preferably, in the flow-through toxicity test apparatus for the local standard test organism, the water quality parameter is preset as follows: the water hardness is 0-300mg/L, the pH is 6-10, the temperature is 15-28 ℃, and the DOC0-10 mg/L.
The beneficial effects of the above technical scheme are: the test water preparation unit can be used for preparing test water with different water quality parameters, the water quality parameters such as water hardness, pH, temperature and DOC can influence the toxic effect of pollutants such as metal, ammonia nitrogen and organic matters on the standard test organisms, the toxicity data of the local standard test organisms under different water quality parameters are required to be obtained in the process of preparing the water quality standard of the aquatic organisms, the water quality parameters can be adjusted through the preparation of the test water, and test water with a series of water quality parameters is provided for the subsequent toxicity test unit after ultraviolet irradiation sterilization.
Preferably, in the above apparatus for testing the flowing water toxicity of the local standard test organism, the flowing water toxicity test unit includes at least seven groups of toxicity test systems, which are respectively a control group, a solvent control group and at least five toxicity concentration gradient groups.
The beneficial effects of the above technical scheme are: each group of toxicity test system consists of a running water type toxicity test which is responsible for the local standard tested organism, a system which treats and recycles the water of the toxicity test system and the like, and the concentration of the test system is ensured.
Preferably, in the above flowing water type toxicity test apparatus for the local reference test organism, each group of the test systems is composed of a plurality of parallel glass containers, preferably each group of the test systems is composed of 3 parallel glass containers, and the glass containers are provided with anti-escape nets with adjustable pore diameters.
The beneficial effects of the above technical scheme are: since standard tests typically provide 3 parallel groups, each group is provided with 3 glass containers. The toxicity test of the native standard tested organism is developed in the glass container, and meanwhile, the solution amount and the aperture of the escape-proof net can be adjusted by combining with the type of the standard tested organism by combining with a liquid level meter arranged in the glass container.
Preferably, in the above-mentioned flowing water type toxicity test apparatus for a local reference test organism, at least 7 reference test organisms are placed in each of the glass containers.
Preferably, in the running water type toxicity test apparatus for the local standard test organism, an illumination system is arranged on the top of the test system.
The beneficial effects of the above technical scheme are: the illumination period and the illumination intensity can be automatically set according to the habit of the reference test organism.
Preferably, in the running water type toxicity test apparatus for the local standard tested organism, the filtering system is provided with a coarse filtering net, a fine filtering net and a fine filtering bag from top to bottom in sequence.
The beneficial effects of the above technical scheme are: the filtering system can effectively remove biological excrement, food residues and the like in a toxicity test solution in a multi-stage filtering mode.
Preferably, in the above-mentioned running water toxicity test apparatus for a local reference test organism, the circulation system comprises a flow rate adjustment system for providing different flow rates of test solutions and an oxygenation system for adjusting the dissolved oxygen level of the water body.
The beneficial effects of the above technical scheme are: the flow rate regulating system can provide test solutions with different flow rates for the benchmark test organisms according to the toxicity test requirements and habits of the benchmark test organisms, such as static, semi-static, flowing and high-flowing, and can regulate the maximum circulation amount to be 100% of water change every 10 minutes; the oxygenation system can adjust the dissolved oxygen level of the water body according to the toxicity test environment and habit of the benchmark tested organism.
According to the technical scheme, compared with the prior art, the invention discloses and provides the running water type toxicity test device for the local reference tested organisms, which can provide a full-automatic test operation platform for the establishment of local aquatic organism references in China and the toxicity test of the local reference tested organisms by scientific research workers, can greatly reduce the manual investment, improve the test repeatability, reduce the uncertainty caused by concentration loss of common static tests and semi-static tests, and solve the problem that the common running water type test device cannot meet the test conditions of the local reference tested organisms except fishes and the test flow required by the aquatic organism references;
the flowing water type toxicity test of the small-sized standard tested crustacean such as Chinese medaka, pseudorasbora, Tang fish, crucian carp, pelteobagrus fulvidraco and the like, freshwater shrimp, Chinese mitten crab and the like of the native standard tested organisms in China can be met, the environment cannot be polluted, and simultaneously, a scientific research worker can autonomously set different water quality parameters and conditions according to the difference of the toxicity test conditions and the difference of habits of the standard tested organisms;
the application of the device can accelerate the formulation of the reference of the aquatic organisms and the related work of the reference tested organism test, reduce the labor cost and human errors in the process, and the device has certain scientific research value and application benefit.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a schematic diagram of the framework of the present invention;
fig. 2 is a schematic overall structure diagram of the invention.
In the figure:
1 is a test water preparation unit, 2 is a running water type toxicity test unit, 21 is a test system, 22 is a circulating system, 221 is a flow rate adjusting system, 222 is an oxygenation system, 23 is a filtering system, 24 is an automatic sampling system, 25 is a water supplementing and automatic dosing system, 26 is an automatic feeding system, 27 is a liquid level meter, 28 is an illumination system, and 3 is a toxicity effect monitoring unit.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The flowing water type toxicity test device of the local reference tested organism in the embodiment is mainly divided into the following three units:
1. test water preparation unit
The test water preparation unit 1 is mainly used for preparing test water and providing the test water for a subsequent running water type toxicity test. The newly prepared standard test water has a pH of 7.8 + -0.2 and a hardness of (250 + -25) mg/L (as CaCO)3Measured), the ratio of Ca/Mg is close to 4:1, the concentration of dissolved oxygen is more than 80 percent of the saturation value of air, and the calcium-magnesium alloy does not contain any substance toxic to tested organisms. Most importantly, the unit can be used for preparing test water with different water quality parameters, the water quality parameters such as water hardness, pH, temperature and DOC can all influence the toxic effect of metal and ammonia nitrogen on the standard test organisms, in the process of establishing the water quality standard of aquatic organisms, the toxicity data of the local standard test organisms under different water quality parameters need to be obtained, the unit can complete the adjustment of the water quality parameters through the preparation of the test water, and the test water is preset: water hardness (the adjusting range is set to be 0-300mg/L), pH (the adjusting range is set to be 6-10), temperature (the adjusting range is set to be 15-28 ℃), DOC (the adjusting range is set to be 0-10mg/L), and test water with a series of water quality parameters is provided for a subsequent test unit after sterilization through ultraviolet irradiation.
2. Running water type toxicity test unit
The flow type toxicity test unit 2 comprises a test system 21, a circulating system 22, a filtering system 23, an automatic sampling system 24, a water supplementing and automatic medicine adding system 25 and an automatic feeding system 26.
The flow type toxicity test unit 2 comprises 9 groups of toxicity test systems (a control group, a solvent control group and seven toxicity concentration gradient groups), each group of units consists of a flow type toxicity test which is responsible for the local standard tested organisms and systems for treating and recycling water used by the toxicity test systems, and the concentration of the test systems is ensured.
(1) Test system
The system mainly comprises 3 parallel glass containers, wherein the toxicity test of local standard test organisms can be developed in the glass containers, 7-10 standard test organisms can be placed in each container, test water is provided by a test water preparation unit 1, and flows out of the test system, enters a filtering system 23 and a circulating system 22 below, enters the test system 21 again through the circulating system 22 to complete one-time circulation, and the quantity and concentration of test solution are monitored by a water supplementing and automatic dosing system 25 in the middle, so that the test solution quantity and concentration of the test system 21 are ensured. The test cylinder is provided with a liquid level meter 27 and an escape prevention net, and the amount of the solution and the aperture of the escape prevention net can be adjusted according to the type of the reference tested organism. The upper part of the test system 21 is provided with an illumination system 28 which can automatically set the illumination period (0-24h) and the illumination intensity according to the habit of the reference test organism.
(2) The filtering system 23 is located below the toxicity test system 21, and the test solution flowing from the test system 21 is mainly filtered by various stages of filtering modes to remove biological feces, food residues and the like in the toxicity test solution.
The unit mainly comprises ① coarse filter screen filtration, ② fine filter screen filtration and ③ fine filter bag filtration.
(3) Circulation system 22 is located below toxicity test system 21, adjacent to filtration system 23, circulation system 22 can provide adjustable flow rate test solutions for flow-through toxicity tests ① flow rate adjustment system 221 (based on toxicity test requirements and habits of a benchmark test subject, test solutions with different flow rates can be provided to the benchmark test subject, such as static, semi-static, flowing, high-flow, adjustable maximum circulation of 100% water change per 10 minutes), ② oxygenation system 222 (based on toxicity test environments and habits of the benchmark test subject, adjusted water dissolved oxygen levels).
(4) The automatic sampling system 24 is located on the left side of the toxicity test system 21, samples the test solution through the mechanical arm sampler 29 within a fixed time, automatically performs instrument analysis on the chemical substance concentration of the taken water sample according to preset chemical analysis conditions, calculates the loss rate of the chemical substances in the test container, and automatically starts the water supplementing and automatic dosing system 25 when the loss exceeds 20%.
(5) The water supplementing and automatic medicine adding system 25 is positioned on the left side of the toxicity test system 21, the water level of the test solution is mainly detected through the liquid level meter 27, and when the water level of the test solution is lower than the normal water level, water is supplemented through the circulating system 22 or the test water preparation unit 1; and when the concentration is lost, the mother liquor is used for quantitatively filling the test system 21 through a sampling mechanical arm to finish the concentration adjustment.
(6) Automatic feeding system
When a chronic test of the local standard test organisms is carried out, bait is automatically added to the test system 21 at regular time according to the weight of the test organisms in a preset percentage, the circulating system 22 is automatically closed when the bait is added, and the circulating system 22 is automatically opened after the bait is added for 1 hour.
3. Toxic effect monitoring unit 3
The unit is arranged on the front side of a toxicity test system 21, real-time infrared behavior and motion scanning of each test system is completed through a preset track, the processes of death, activity, ingestion, growth, reproduction and the like of a reference tested organism are identified, the conditions of death, activity and the like of the reference tested organism are automatically recorded, analysis and calculation are carried out, and meanwhile, a mechanical arm on the outer side is used for fishing out the dead organisms.
Note that: the test water is water for preparing a test solution and does not contain other toxic pollutants; the test solution is a solution obtained by adding a mother solution containing a pollutant to test water to directly perform a toxicity test on organisms such as fish.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (9)
1. A running water type toxicity test device of a local standard tested organism is characterized by comprising a test water preparation unit, a running water type toxicity test unit and a toxicity effect monitoring unit, wherein the test water preparation unit is used for providing test water with different water quality parameters, and the toxicity effect monitoring unit is used for identifying the growth state of the standard tested organism;
the flow type toxicity test unit comprises a plurality of groups of toxicity test systems, wherein each group of toxicity test system comprises a test system for carrying out toxicity test, a filtering system for filtering test solution, a circulating system for adjusting the flow rate of the test solution, an automatic sampling system for sampling and monitoring chemical substances in the test solution, a water supplementing and automatic medicine adding system for adjusting the water volume/concentration of the test solution, and an automatic feeding system for adding bait into the test system;
the input end of the test system is connected with the test water preparation unit;
the filtering system and the circulating system are both positioned below the testing system, and the testing system is respectively communicated with the filtering system and the circulating system;
the automatic sampling system is positioned at one side of the test system, samples the test solution in the test system through a mechanical arm within a fixed time, and is electrically connected with the water supplementing and automatic dosing system;
the water supplementing and automatic dosing system is positioned on one side of the test system, the water level of the test solution in the test system is detected through a liquid level meter, and the test water preparation unit and the circulating system are controlled to supplement water through a detection result;
the automatic feeding system is electrically connected with the circulating system;
the toxicity effect monitoring unit is used for performing infrared behavior scanning on the test system, and is electrically connected with the automatic sampling system.
2. The apparatus according to claim 1, wherein the test water preparation unit adjusts water quality parameters of the test water including but not limited to water hardness, pH, temperature, and dissolved organic carbon DOC.
3. The running water type toxicity test apparatus for the in-situ standard test organism according to claim 2, wherein the water quality parameters are set within a range of: the water hardness is 0-300mg/L, the pH is 6-10, the temperature is 15-28 ℃, and the DOC is 0-10 mg/L.
4. The apparatus according to claim 1, wherein the flow-through toxicity test unit comprises at least seven groups of toxicity test systems, each of which is a control group, a solvent control group, and at least five toxicity concentration gradient groups.
5. The running water type toxicity test device for the local reference tested organisms according to claim 1, wherein each group of test systems is composed of a plurality of parallel glass containers, and the glass containers are provided with anti-escape nets with adjustable pore diameters.
6. The apparatus according to claim 4, wherein at least 7 reference test organisms are placed in each glass container.
7. The running water type toxicity test device for the local reference tested organisms according to claim 1, wherein an illumination system is arranged on the top of the test system.
8. The running water type toxicity test device of the local reference tested organism according to claim 1, wherein the filtering system is provided with a coarse filtering net of 1mm, a fine filtering net of 0.1mm and a fine filtering bag of 0.010mm from top to bottom in sequence.
9. The apparatus of claim 1, wherein the circulation system comprises a flow rate adjustment system for providing different flow rates of test solution and an oxygenation system for adjusting the dissolved oxygen level of the water.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911184090.3A CN110763816A (en) | 2019-11-27 | 2019-11-27 | Flowing water type toxicity test device for native reference tested organisms |
| AU2020101177A AU2020101177A6 (en) | 2019-11-27 | 2020-06-29 | Flow-type toxicity test device for aquatic life criteria testing organisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911184090.3A CN110763816A (en) | 2019-11-27 | 2019-11-27 | Flowing water type toxicity test device for native reference tested organisms |
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| CN110763816A true CN110763816A (en) | 2020-02-07 |
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| CN201911184090.3A Pending CN110763816A (en) | 2019-11-27 | 2019-11-27 | Flowing water type toxicity test device for native reference tested organisms |
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| AU (1) | AU2020101177A6 (en) |
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| CN113884413A (en) * | 2021-08-25 | 2022-01-04 | 中国环境科学研究院 | Method for testing toxicity effect of gasoline vehicle exhaust particles on aquatic organisms |
| CN114324847B (en) * | 2022-01-14 | 2022-06-24 | 生态环境部南京环境科学研究所 | Method for testing acute toxicity of rice disease and pest control agent on procambarus clarkii |
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| AU2020101177A6 (en) | 2020-09-17 |
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