AU2020101177A6 - Flow-type toxicity test device for aquatic life criteria testing organisms - Google Patents

Flow-type toxicity test device for aquatic life criteria testing organisms Download PDF

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AU2020101177A6
AU2020101177A6 AU2020101177A AU2020101177A AU2020101177A6 AU 2020101177 A6 AU2020101177 A6 AU 2020101177A6 AU 2020101177 A AU2020101177 A AU 2020101177A AU 2020101177 A AU2020101177 A AU 2020101177A AU 2020101177 A6 AU2020101177 A6 AU 2020101177A6
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test
water
flow
toxicity
automatic
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AU2020101177A4 (en
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Jin Chen
Bo Fan
Ji Li
Zhengtao Liu
Xiaonan WANG
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Chinese Research Academy of Environmental Sciences
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/18—Water
    • G01N33/186—Water using one or more living organisms, e.g. a fish
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01D—MEASURING NOT SPECIALLY ADAPTED FOR A SPECIFIC VARIABLE; ARRANGEMENTS FOR MEASURING TWO OR MORE VARIABLES NOT COVERED IN A SINGLE OTHER SUBCLASS; TARIFF METERING APPARATUS; MEASURING OR TESTING NOT OTHERWISE PROVIDED FOR
    • G01D21/00—Measuring or testing not otherwise provided for
    • G01D21/02—Measuring two or more variables by means not covered by a single other subclass

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  • Life Sciences & Earth Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
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Abstract

The present invention discloses a flow-type toxicity test device for aquatic biological reference testing organisms, which comprises three units such as a test water preparation unit, a flow-type toxicity test unit and a toxicity effect monitoring unit, wherein the flow-type toxicity test unit comprises a test system, a circulation system, a filtration system, an automatic sampling system, a water replenishing and automatic dosing system, and an automatic feeding system. Different water quality parameters and conditions can be set independently according to the differences in the toxicity test conditions and the differences in habits of the reference testing organisms, to meet the flow-type toxicity test of the aquatic biological reference testing organisms, without polluting the environment. The application of the device can accelerate the formulation of aquatic organism references and related work of reference testing organisms, reduces labor cost and human errors in the process, and has good scientific research value and application benefits. Drawings of Description Test water preparation unit 1 Test sys Flowrate adjusting system 221 0 -- Circulation system 22 Oxygenation system222 -Filtration system 23 - Flow-type toxicity test unit 2 Automatic sampling system 24 Water replenishing and automatic dosmeL system 25 Automatic teedmng 3 ----~- system 26 CL Toxicity effect Liquid level gauge 27 monitorina unit 3 -Lighting system 28 Fig. 1 1

Description

Drawings of Description
Test water preparation unit 1
Test sys Flowrate adjusting system 221
0 -- Circulation system 22
Oxygenation system222 -Filtration system 23
- Flow-type toxicity test unit 2 Automatic sampling system 24
Water replenishing and automatic dosmeL system 25
Automatic teedmng CL 3 ----~- system 26
Toxicity effect Liquid level gauge 27 monitorina unit 3
-Lighting system 28
Fig. 1
Description
FLOW-TYPE TOXICITY TEST DEVICE FOR AQUATIC LIFE CRITERIA TESTING ORGANISMS
Technical Field
The present invention relates to the technical field of aquatic life criteria tests and ecotoxicology, and more particularly relates to a flow-type toxicity test device for aquatic life criteria testing organisms.
Background
A flow-type toxicity test is a test to inspect the influence of pollutants on aquatic biological toxicity in a continuously flowing solution. The test solution and the control solution continuously flow into a test container containing testing organisms through a water replenishing and automatic dosing system; and the concentration of the pollutants remains stable throughout the test. The ecotoxicology test of aquatic organisms is an important part of conducting criteria research work on the aquatic organisms. However, there is no unified flow-type toxicity test device for aquatic life criteria testing organisms in China and abroad, thereby failing to meet the test conditions of the testing organisms other than fish and the test procedures required by the aquatic life criteria. Specifically, the existing flow-type devices are generally international standard testing organisms for chemical testing, such as zebrafish, and the like, and are all for fish, not for small animals such as Daphnia magna. With the development of a water quality criteria research system, a large amount of testing work on the testing organisms is also required. Therefore, how to provide a flow-type toxicity test device that can test the fish and especially the small animals is a problem that needs to be urgently solved by those skilled in the art.
Description
Summary In view of this, the present invention provides a flow-type toxicity test device which can be used for small aquatic life criteria testing species, such as juvenile fish of Japanese medaka, Oryzias latipes sinensis, Pseudorasbora parva, Tanichthys albonubes, crucian carp, carp, yellow catfish, zebrafish and rainbow trout, and crustaceans of shrimp, crabs, Daphnia magna, etc. Moreover, the present invention does not pollute the environment, and has good scientific research value and application benefits. In order to achieve the above purpose, the present invention adopts the following technical solutions: A flow-type toxicity test device for aquatic life criteria testing organisms comprises: a test water preparation unit for providing test water with different water quality parameters, a flow-type toxicity test unit and a toxicity effect monitoring unit for identifying growth states of testing organisms, wherein the flow-type toxicity test unit comprises a plurality of groups of toxicity test systems; each group of toxicity test systems comprises a test system for conducting a toxicity tests, a filtration system for filtering a test solution, a circulation system for adjusting the flow rate of the test solution, an automatic sampling system for sampling and monitoring chemical substances in the test solution, a water replenishing and automatic dosing system for adjusting the water quantity/concentration of the test solution, and an automatic feeding system for feeding a bait for the test system. It should be noted that the test system, the filtration system, and the circulation system are independently arranged for each group of toxicity test systems, while the water replenishing and automatic dosing system, the automatic sampling system and the automatic feeding system are jointly used by a plurality
Description
of groups of toxicity test systems, thereby not only realizing the normal operation of the test, but also saving the space to the greatest extent. An input end of the test system is connected with the test water preparation unit. The filtration system and the circulation system are located below the test system, and the test system is respectively communicated with the filtration system and the circulation system. The automatic sampling system is located on one side of the test system; the test solution in the test system is sampled by a mechanical arm within a fixed time; and the automatic sampling system is electrically connected with the water replenishing and automatic dosing system. The water replenishing and automatic dosing system is located on one side of the test system; a water level of the test solution in the test system is detected by a liquid level gauge; and the test water preparation unit and the circulation system are controlled by test results to replenish water. The automatic feeding system is electrically connected with the circulation system. The toxicity effect monitoring unit is used to perform infrared behavior scanning on the test system, and the toxicity effect monitoring unit is electrically connected with the automatic sampling system. Preferably, in the above flow-type toxicity test device for aquatic life criteria testing organisms, the test water preparation unit adjusts the water quality parameters of the test water, comprising but not limited to water hardness, pH, temperature, and dissolved organic carbon DOC. The beneficial effects of the above technical solution are: the test water preparation unit is used for the preparation of test water and providing a test solution for subsequent flow-type toxicity tests, and does not contain any substance that is toxic to the testing organism. Preferably, in the above flow-type toxicity test device for aquatic life criteria testing organisms, the water quality parameters are preset as follows: water
Description
hardness of 0-300 mg/L, pH of 6-10, temperature of 15-28°C and DOC of 0-10 mg/L. The beneficial effects of the above technical solution are: the test water preparation unit can prepare test water with different water quality parameters. The water quality parameters such as water hardness, pH, temperature and DOC may affect the toxic effects of metals, ammonia nitrogen, organic matter and other pollutants on the testing organisms. In the process of formulating water quality criteria for aquatic organisms, it is necessary to obtain toxicity data of the testing organisms under different water quality parameters. The unit can complete the adjustment of the water quality parameters through the preparation of the test water. After sterilization by ultraviolet irradiation, a series of test water with water quality parameters are provided for subsequent toxicity test units. Preferably, in the above flow-type toxicity test device for aquatic life criteria testing organisms, the flow-type toxicity test unit comprises at least seven groups of toxicity test systems, which are respectively a control group, a solvent control group and at least five toxicity concentration gradient groups. The beneficial effects of the above technical solution are: each group of toxicity test system is composed of systems responsible for the flow-type toxicity test of the testing organisms and the treatment and recycling of the water used in the toxicity test system, and guarantees the concentration of the test system. Preferably, in the above flow-type toxicity test device for aquatic life criteria testing organisms, each group of the test systems is composed of a plurality of parallel glass containers; preferably, each group of the test systems is composed of three parallel glass containers; and the glass containers are provided with anti-escape nets with adjustable apertures. The beneficial effects of the above technical solution are: since the standard test generally sets three parallel groups, each group is provided with three glass containers. The toxicity test of the testing organisms is conducted in the glass containers; and meanwhile, combined with the liquid level gauges placed in the
Description
glass containers, the amount of the solution and the apertures of the anti-escape nets can be adjusted in combination with the types of the testing organisms. Preferably, in the above flow-type toxicity test device for aquatic life criteria testing organisms, at least seven testing organisms are put into each of the glass containers. Preferably, in the above flow-type toxicity test device for aquatic life criteria testing organisms, the top of each test system is provided with a lighting system. The beneficial effects of the above technical solution are: a lighting cycle and lighting intensity can be automatically set according to the habits of the testing organisms. Preferably, in the above flow-type toxicity test device for aquatic life criteria testing organisms, the filtration system is provided with a coarse filter screen, a fine filter screen and a refined filter bag successively from top to bottom. The beneficial effects of the above technical solution are: the filtration system can effectively remove biological feces and food residues in the toxicity test solution through a multi-stage filtration mode. Preferably, in the above flow-type toxicity test device for aquatic life criteria testing organisms, the circulation system comprises a flow rate adjusting system for providing test solutions with different flow rates and an oxygenation system for adjusting a dissolved oxygen level of water. The beneficial effects of the above technical solution are: the flow rate adjusting system can provide test solutions with different flow rates, such as static, semi-static, flowing, and high-flow solutions, for the testing organisms based on the toxicity test needs and habits of the testing organisms; a maximum circulation amount can be adjusted as 100% water change every 10 minutes; and the oxygenation system can adjust the dissolved oxygen level of water according to the toxicity test environments and habits of the testing organisms. It can be known from the above technical solutions that, compared with the prior art, the present invention discloses and provides a flow-type toxicity test device for aquatic life criteria testing organisms, which can provide a fully
rN
Description
automatic test operation platform for the establishment of aquatic life criteria and the toxicity tests on the testing organisms by scientific researchers, can greatly reduce the labor input, can improve the repeatability of the test, can also reduce the uncertainty caused by the concentration loss of ordinary static tests and semi-static tests, and can solve the problem that a common flow-type test device fails to meet the test conditions of the testing organisms other than fish and the test procedures required by the aquatic life criteria. The present invention can be used for the flow-type toxicity tests of small aquatic life criteria testing organisms such as juvenile fish of Japanese medaka, Oryzias latipes sinensis, Pseudorasboraparva, Tanichthys albonubes, crucian carp, carp, yellow catfish, zebrafish and rainbow trout, and crustaceans of shrimp, crabs, Daphnia magna, etc., and does not pollute the environment. Meanwhile, different water quality parameters and conditions can be set independently by the scientific researchers according to the differences in the toxicity test conditions and the differences in habits of the testing organisms. The application of the device can accelerate the formulation of aquatic life criteria and related work of the testing organisms, reduces labor cost and human errors in the process, and has certain scientific research value and application benefits.
Description of Drawings
To more clearly describe the technical solutions in the embodiments of the present invention or in the prior art, the drawings required to be used in the description of the embodiments or the prior art will be simply presented below. Apparently, the drawings in the following description are merely some embodiments of the present invention, and for those ordinary skilled in the art, other drawings can also be obtained according to these drawings without contributing creative labor.
I;
Description
Fig. 1 is a schematic diagram of a frame structure of the present invention; and Fig. 2 is a schematic diagram of an overall structure of the present invention. In the figures: 1 test water preparation unit; 2 flow-type toxicity test unit; 21 test system; 22 circulation system; 221 flow rate adjusting system; 222 oxygenation system; 23 filtration system; 24 automatic sampling system; 25 water replenishing and automatic dosing system; 26 automatic feeding system; 27 liquid level gauge; 28 lighting system; and 3 toxicity effect monitoring unit.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and fully described below. Apparently, the described embodiments are merely part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those ordinary skilled in the art without contributing creative labor will belong to the protection scope of the present invention. A flow-type toxicity test device for aquatic life criteria testing organisms in the present embodiment is mainly composed of the following three units: 1. Test water preparation unit The test water preparation unit 1 is mainly used for the preparation of test water and providing test water for subsequent flow-type toxicity tests. The prepared standard test water has a pH value of 7.80.2, a hardness of (25025) mg/L (calculated as CaCO3), a Ca/Mg ratio close to 4:1, and a dissolved oxygen concentration of more than 80% of the air saturation value, and does not contain any substance that is toxic to the testing organisms. It is most important that the unit can prepare the test water with different water quality parameters. The water quality parameters such as water hardness, pH, temperature and DOC may affect the toxic effects of metals and ammonia nitrogen on the testing organisms. In the
Description
process of formulating water quality criteria for aquatic organisms, it is necessary to obtain toxicity data of the testing organisms under different water quality parameters. The unit can complete the adjustment of the water quality parameters through the preparation of the test water. The test water is preset as follows: water hardness (the adjusting range is set as 0-300 mg/L), pH (the adjusting range is set as 6-10), temperature (the adjusting range is set as 15-28°C) and DOC (the adjusting range is set as 0-10 mg/L). After sterilization by ultraviolet irradiation, a series of test water with water quality parameters are provided for subsequent test units. 2. Flow-type toxicity test unit The flow-type toxicity test unit 2 comprises a test system 21, a circulation system 22, a filtration system 23, an automatic sampling system 24, a water replenishing and automatic dosing system 25, and an automatic feeding system 26. The flow-type toxicity test unit 2 comprises ninth groups of toxicity test systems (a control group, a solvent control group and seven toxicity concentration gradient groups). Each group of the units is composed of systems responsible for the flow-type toxicity test of the testing organisms and the treatment and recycling of the water used in the toxicity test system, and guarantees the concentration of the test system. (1) Test system The system is mainly composed of three parallel glass containers. The toxicity test of the aquatic life criteria testing organisms can be conducted in the glass containers. Seven to ten testing organisms can be put into each of the containers. The test water is provided by the test water preparation unit 1. After flowing out of the test system, the test water enters the filtration system 23 and the circulation system 22 below, and enters the test system 21 again through the circulation system 22 to complete a cycle. In the process, the water replenishing and automatic dosing system 25 monitors the amount and the concentration of the test solution to ensure the amount and the concentration of the test solution of the test system 21. A liquid level gauge 27 and an anti-escape net are arranged in a test cylinder. The
Description
amount of the solution and the aperture of the anti-escape net can be adjusted according to the types of the testing organisms. The top of the test system 21 is provided with a lighting system 28. A lighting cycle (0-24 h) and lighting intensity can be automatically set according to the habits of the testing organisms. (2) The filtration system 23 is located below the toxicity test system 21. The test solution which flows from the test system 21 is filtered mainly through a multi-stage filtration mode to remove biological feces and food residues in the toxicity test solution. The unit is mainly composed of ( a coarse filter screen, @ a fine filter screen and @ a refined filter bag. (3) The circulation system 22 is located below the toxicity test system 21 and is adjacent to the filtration system 23. The circulation system 22 can provide a test solution with adjustable flow rate for the flow-type toxicity test. 0 The flow rate adjusting system 221 (can provide test solutions with different flow rates, such as static, semi-static, flowing, and high-flow solutions, for the testing organisms based on the toxicity test needs and habits of the testing organisms; and a maximum circulation amount can be adjusted as 100% water change every 10 minutes) @ The oxygenation system 222 (can adjust the dissolved oxygen level of water according to the toxicity test environments and habits of the testing organisms). (4) The automatic sampling system 24 is located on the left side of the toxicity test system 21; the test solution is sampled by a mechanical arm sampler 29 within a fixed time; the concentration of a chemical substance in a water sample is automatically analyzed by the instrument according to the preset chemical analysis conditions. The loss rate of the chemical substance in the test container is calculated. When the loss exceeds 20%, the water replenishing and automatic dosing system 25 is automatically started. (5) The water replenishing and automatic dosing system 25 is located on the left side of the toxicity test system 21, and the water level of the test solution is mainly detected by the liquid level gauge 27. When the water level of the test
Description
solution is lower than a normal water level, the water is replenished through the circulation system 22 or the test water preparation unit 1; and when the concentration is lost, a mother solution is used to quantitatively fill the test system 21 through the sampling mechanic arm to complete the concentration adjustment. (6) Automatic feeding system When a chronic test of the aquatic life criteria testing organisms is conducted, the test system 21 is automatically fed with baits at regular time in a preset percentage according to the weight of the testing organisms. When the baits are fed, the circulation system 22 is automatically closed, and after the baits are fed for 1 h, the circulation system 22 is automatically started. 3. Toxicity effect monitoring unit The unit is prepared on the front side of the toxicity test system 21, completes real-time infrared behaviors and motion scanning of each test system through the preset tracks, recognizes the processes of death, motion, feeding, growth and reproduction of the testing organisms, automatically records the death, the motion and other conditions of the testing organisms, and conducts analysis and calculation; and meanwhile, the outer mechanical arm takes out the dead organisms. Note: The above test water refers to the water used to configure the test solution, and does not contain other toxic pollutants. The test solution refers to a solution in which a mother solution containing the pollutants is added to the test water to directly conduct the toxicity test on fish and other organisms. Each embodiment in the description is described in a progressive way. The difference of each embodiment from each other is the focus of explanation. The same and similar parts among all of the embodiments can be referred to each other. For the device disclosed by the embodiments, because the device corresponds to a method disclosed by the embodiments, the device is simply described. Refer to the description of the method part for the related part. The above description of the disclosed embodiments enables those skilled in the art to realize or use the present invention. Many modifications made to these
1 (
Description
embodiments will be apparent to those skilled in the art. General principles defined herein can be realized in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims (5)

Claims
1. A flow-type toxicity test device for aquatic life criteria testing organisms, comprising a test water preparation unit for providing test water with different water quality parameters, a flow-type toxicity test unit and a toxicity effect monitoring unit for identifying growth states of testing organisms, wherein the flow-type toxicity test unit comprises a plurality of groups of toxicity test systems; each group of toxicity test systems comprises a test system for conducting a toxicity tests, a filtration system for filtering a test solution, a circulation system for adjusting the flow rate of the test solution, an automatic sampling system for sampling and monitoring chemical substances in the test solution, a water replenishing and automatic dosing system for adjusting the water quantity/concentration of the test solution, and an automatic feeding system for feeding a bait for the test system; an input end of the test system is connected with the test water preparation unit; the filtration system and the circulation system are located below the test system, and the test system is respectively communicated with the filtration system and the circulation system; the automatic sampling system is located on one side of the test system; the test solution in the test system is sampled by a mechanical arm within a fixed time; and the automatic sampling system is electrically connected with the water replenishing and automatic dosing system; the water replenishing and automatic dosing system is located on one side of the test system; a water level of the test solution in the test system is detected by a liquid level gauge; and the test water preparation unit and the circulation system are controlled by test results to replenish water; the automatic feeding system is electrically connected with the circulation system; the toxicity effect monitoring unit is used to perform infrared behavior scanning on the test system, and the toxicity effect monitoring unit is electrically connected with the automatic sampling system.
Claims
2. The flow-type toxicity test device for aquatic life criteria testing organisms according to claim 1, wherein the test water preparation unit adjusts the water quality parameters of the test water, comprising but not limited to water hardness, pH, temperature, and dissolved organic carbon DOC; the setting range of the water quality parameters is: water hardness of 0-300 mg/L, pH of 6-10, temperature of 15-28°C and DOC of 0-10 mg/L.
3. The flow-type toxicity test device for aquatic life criteria testing organisms according to claim 1, wherein the flow-type toxicity test unit comprises at least seven groups of toxicity test systems, which are respectively a control group, a solvent control group and at least five toxicity concentration gradient groups.
4. The flow-type toxicity test device for aquatic life criteria testing organisms according to claim 1, wherein each group of the test systems is composed of a plurality of parallel glass containers, and the glass containers are provided with anti-escape nets with adjustable apertures; at least seven testing organisms are put into each of the glass containers; the top of each test system is provided with a lighting system.
5. The flow-type toxicity test device for aquatic life criteria testing organisms according to claim 1, wherein the filtration system is provided with a coarse filter screen of 1 mm, a fine filter screen of 0.1 mm and a refined filter bag of 0.010 mm successively from top to bottom; the circulation system comprises a flow rate adjusting system for providing test solutions with different flow rates and an oxygenation system for adjusting a dissolved oxygen level of water.
AU2020101177A 2019-11-27 2020-06-29 Flow-type toxicity test device for aquatic life criteria testing organisms Ceased AU2020101177A6 (en)

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CN113884413A (en) * 2021-08-25 2022-01-04 中国环境科学研究院 Method for testing toxicity effect of gasoline vehicle exhaust particles on aquatic organisms
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