CN1358864A - Additives for ferment of spiramycin - Google Patents
Additives for ferment of spiramycin Download PDFInfo
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- CN1358864A CN1358864A CN 01129171 CN01129171A CN1358864A CN 1358864 A CN1358864 A CN 1358864A CN 01129171 CN01129171 CN 01129171 CN 01129171 A CN01129171 A CN 01129171A CN 1358864 A CN1358864 A CN 1358864A
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- additive
- vitamin
- phytic acid
- hydrolysis
- stage casing
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Abstract
The present invention discloses a new additive for fermentation of spiramycin. It is made from plant protein raw material, and processed by hydrolysis, neutralizing, decolouring, desalting, splitting, adding a certain quantity of phytic acid and biotin, spray-drying to obtain the invented product. Said invented additive richly contains four beneficial amino acids, and contains phytic acid capable of absorbing ammonia produced in the course of fermentation and biotin capable of promoting growth. Said method can obviously raise fermentation unit and can reduce production cost.
Description
Technical field
The present invention relates to the new additive agent that supplies ferment of spiramycin to use that the specific preparation method of a kind of usefulness makes, belong to the antibiotic fermentation technical field.
Background technology
In the prior art, the nitrogenous source material that uses vegetable-protein such as dregs of beans directly to use as ferment of spiramycin does not usually have specific aim to ferment of spiramycin, and the anti-mechanism of the product of Spiramycin Base is lacked further investigation.After deliberation, find to have only Threonine in the contained multiple amino acids of plant protein material such as dregs of beans, four kinds of Xie Ansuan, leucine and Isoleucines have active effect to ferment of spiramycin, and also many to the demand of this four seed amino acid; But because the component content ratio of dregs of beans etc. is more fixing, four kinds of useful amino acid whose interpolation demand height, other amino acid whose addition is also corresponding to be increased, cause the nitrogenous source total amount too high, then making the objectionable impurities that produces in the fermenting process---the amount of ammonia is too high, ammonia is too high to produce the anti-restraining effect that has to Spiramycin Base, so the fermentation unit of manufacturer is not high at present, and the production cost height.
Summary of the invention
At the ferment of spiramycin that exists in the prior art with the problem that has too much ammonia to produce in nitrogenous source material shortage specific aim and the fermenting process and consider that Spiramycin Base produces anti-needs, the object of the present invention is to provide a kind of Threonine, Xie Ansuan, leucine and four kinds of useful amino acid of Isoleucine of being rich in, and amino acid containedly all exist, and also contain phytic acid that can absorb the ammonia that produces in the fermenting process and the ferment of spiramycin new additive agent that promotes the vitamin H of growth with free shape form.
The objective of the invention is by adopting following method to be prepared into that desired additives realizes.Be additives for ferment of spiramycin of the present invention be adopt with plant protein material (as dregs of beans) carry out acid hydrolysis handle hydrolyzed solution, hydrolyzed solution is cooled to 50～65 ℃ then, the elimination throw out, after neutralization, decolouring are handled, spent ion exchange resin desalination again, get the stage casing effluent liquid, add a certain amount of phytic acid and vitamin H therein, last spray-dried processing and making.Additive of the present invention be plant protein material through hydrolysis, filter, remove slag, neutralize, make after the series of processes such as decolouring, desalination, it includes four kinds of a large amount of useful amino acid, and amino acid all is free shape, just has stronger specific aim with this material as additives for ferment, can suitably control the nitrogenous source total amount, reduce objectionable impurities---the amount of ammonia in the fermenting process; Have again and contain phytic acid in the finished product, can absorb the ammonia that produces in the fermenting process, further reduce the quantum of output of ammonia, get rid of ammonia Spiramycin Base is produced anti-restraining effect; Simultaneously, the vitamin H in the goods also can promote fermentation.The common synergism of above-mentioned nitrogenous source material effective constituent can obviously improve the fermentation unit of Spiramycin Base, reduces production costs.
In each the method for above-mentioned system, the add-on of phytic acid and vitamin H is pressed resin desalination stage casing effluent liquid: phytic acid: vitamin H=2～3: 4～5: 3～4 (weight ratios), the best are that 3: 4: 3 ratio adds.
Above-mentioned plant protein material hydrochloric acid hydrolysis treatment process is meant plant protein material (as dregs of beans) by solid-to-liquid ratio 1: 1～2 (weight ratio), best solid-to-liquid ratio is 1: 1.5～1.7, joining concentration is in the hydrochloric acid of 20～45% (the best is 30～35%), under normal pressure,, obtain hydrolyzed solution in 100～115 ℃ of (the best is 104-110 ℃) azeotropic hydrolysis 6～10 hours.
In above-mentioned and in the operation, adopt ammoniacal liquor (NH
3H
2O) handle, making the hydrolyzed solution pH value after removing slag is 5.2～5.8, and best pH value is 5.5.
In the above-mentioned bleaching process, adopt decolorizing with activated carbon to handle, be faint yellow to the liquid look.
Drying treatment mode of the present invention is a spraying drying; The plant protein material that adopts is inexpensive dregs of beans.
By analysis, the component content of stage casing of the present invention effluent liquid intermediates is:
(1) four kind of useful amino acid whose content: 6～7%
(2) other amino acid whose content: 12～13%
(3) content of crude protein: 3～7%
(4) Cl
-Content: 5～7%
(5) NH
4 +Content: 0.3～0.7%
(6) the ash content of coal: 1～2%
(7) harmful element content: 8～15ppm
As seen, the intermediates of this stage casing effluent liquid contain a large amount of four kinds of useful amino acid (Threonine, Xie Ansuan, leucine and Isoleucine) and amino acid and all are unbound state.This intermediates are by resin desalination stage casing effluent liquid: phytic acid: vitamin H=2～3: 4～5: 3～4 (weight ratios) make the product of finally making of the present invention be rich in four kinds of useful amino acid whose whiles after adding phytic acid and vitamin H, also contain a large amount of phytic acid and vitamin H, ferment of spiramycin is highly advantageous to.The fermentation unit that adopts dregs of beans is generally at 3000～3500 μ/ml, and adopts new additive agent of the present invention, ferment of spiramycin unit can be brought up to 4500 μ/more than the ml, thereby enhance productivity, reduces production costs.In addition, the preparation technology of the present invention's employing has the characteristics that technology is simple, each operation is easy to control and operate, and can promote the antibiotic fermentation starting material to specialized, the deep processing that becomes more meticulous development, and it is blank to fill up domestic research and production in this respect.
Embodiment
Embodiment 1: getting the 1000kg dregs of beans, to join 1500kg concentration be in 35% the hydrochloric acid, to heat under the normal pressure to 105 ℃ of azeotropic hydrolysis 8 hours, obtains hydrolyzed solution, be cooled to 55 ℃ after the elimination throw out, (adopt ammoniacal liquor NH with alkali lye
3H
2O) neutralizing treatment, making the hydrolyzed solution pH value after removing slag is 5.2～5.8 (best pH value is 5.5), removes look with gac then, makes liquid be faint yellow, spent ion exchange resin desalination again (is removed NH
4Cl etc.), get stage casing effluent liquid (its pH value is between 3.5～8.5) at last and press the stage casing effluent liquid: phytic acid: vitamin H=2～3: 4～5: 3～4 (weight ratios), optimum proportion is the phytic acid and the vitamin H of 3: 4: 3 adding chemical grades, the spray-dried meal that obtains is promptly made product of the present invention.
Embodiment 2: getting the 1000kg dregs of beans, to join 1700kg concentration be in 30% the hydrochloric acid, to heat under the normal pressure to 110 ℃ of azeotropic hydrolysis 10 hours, obtains hydrolyzed solution; Elimination precipitation slag thing after this hydrolyzed solution is cooled to 60 ℃ is through with ammoniacal liquor (NH
3H
2O) neutralizing treatment, making the solution pH value is 5.5, handle to solution with activated carbon decolorizing to be faint yellow, and then spent ion exchange resin removes salt (NH again
4Cl etc.), get stage casing effluent intermediates at last by the stage casing effluent: phytic acid: vitamin H=2～3: 4～5: 3～4 (weight ratios), optimum proportion is to add the phytic acid and the vitamin H of chemical grade at 3: 4: 3, spray-driedly obtains being rich in four kinds of useful amino acid whose meals and promptly makes product of the present invention.
Claims (10)
1. additives for ferment of spiramycin, it is characterized in that it is to adopt plant protein material to carry out the acid hydrolysis processing to obtain hydrolyzed solution, then hydrolyzed solution is cooled to 50～65 ℃ of elimination precipitations, after neutralization, decolouring are handled, spent ion exchange resin desalination again, get the stage casing effluent liquid and mix, the prepared new additive agent of spray drying treatment with a certain amount of phytic acid and vitamin H.
2. additive according to claim 1, the add-on that it is characterized in that phytic acid and vitamin H are in resin desalination stage casing effluent liquid: the ratio of phytic acid: vitamin H=2～3: 4-5: 3-4 (weight ratio) adds.
3. as additive as described in the claim 2,, it is characterized in that resin desalination stage casing effluent liquid: phytic acid: the ratio of vitamin H is 3: 4: 3 (weight ratio).
4. additive according to claim 1, the hydrochloric acid hydrolysis that it is characterized in that plant protein material is handled and to be meant that it is in 20～45% the hydrochloric acid that plant protein material is joined concentration by solid-to-liquid ratio 1: 1～2 (weight ratio), obtains hydrolyzed solution in 6～10 hours in 100～115 ℃ of azeotropic hydrolysis under normal pressure.
5. as additive as described in the claim 4, it is characterized in that plant protein material and hydrochloric acid solution blended solid-to-liquid ratio are 1: 1.5～1.7 (weight ratios).
6. as additive as described in the claim 4, the concentration that it is characterized in that the hydrochloric acid solution that hydrolysis is used is 30～35%.
7. as additive as described in the claim 4, the temperature that it is characterized in that hydrolysis treatment is 104～110 ℃.
8. additive according to claim 1 is characterized in that adopting the ammoniacal liquor neutralizing treatment, and making the hydrolyzed solution pH value after removing slag is 5.2～5.8.
9. additive according to claim 1 is characterized in that adopting activated carbon decolorizing to handle, and makes liquid be faint yellow.
10. additive according to claim 1 is characterized in that the mixture of stage casing effluent and phytic acid and vitamin H adopts spray drying treatment to make meal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011291710A CN1151266C (en) | 2001-12-10 | 2001-12-10 | Additives for ferment of spiramycin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011291710A CN1151266C (en) | 2001-12-10 | 2001-12-10 | Additives for ferment of spiramycin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1358864A true CN1358864A (en) | 2002-07-17 |
| CN1151266C CN1151266C (en) | 2004-05-26 |
Family
ID=4668963
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB011291710A Expired - Fee Related CN1151266C (en) | 2001-12-10 | 2001-12-10 | Additives for ferment of spiramycin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1151266C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100402659C (en) * | 2004-02-17 | 2008-07-16 | 窦德献 | Ferment process of anti-feedback inhibition and application in producing antibiotics thereof |
| CN100465283C (en) * | 2003-12-12 | 2009-03-04 | 华东理工大学 | Bitespiramycin yield increase fermentation process by adding metal ion |
-
2001
- 2001-12-10 CN CNB011291710A patent/CN1151266C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100465283C (en) * | 2003-12-12 | 2009-03-04 | 华东理工大学 | Bitespiramycin yield increase fermentation process by adding metal ion |
| CN100402659C (en) * | 2004-02-17 | 2008-07-16 | 窦德献 | Ferment process of anti-feedback inhibition and application in producing antibiotics thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1151266C (en) | 2004-05-26 |
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Owner name: HEFENG BIOLOGY ENGINEERING CO., LTD., JIANYANG CI Free format text: FORMER OWNER: LI JIANFENG Effective date: 20080516 |
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Effective date of registration: 20080516 Address after: Jianyang ten mile dam Industrial Park Patentee after: Jianyang Hefeng Bio-Engineering Co., Ltd. Address before: Sichuan city of Chengdu province Yulin Lane No. 3 Patentee before: Li Jianfeng |
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