CN1357360A - Hypoglycemic biopreparation - Google Patents
Hypoglycemic biopreparation Download PDFInfo
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- CN1357360A CN1357360A CN01130450A CN01130450A CN1357360A CN 1357360 A CN1357360 A CN 1357360A CN 01130450 A CN01130450 A CN 01130450A CN 01130450 A CN01130450 A CN 01130450A CN 1357360 A CN1357360 A CN 1357360A
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Abstract
The hypoglycemic biopreparation consists of several microbe coloies and their metabolic product and is prepared through conventional activation, enlarged culture, fermentation, filtering, dregs elimination, disinfection, freeze drying and other steps. The biopreparation can regulate body's metabolic function comprehensively, repair pathological insulin tissue and restore its physiological function, promote insulin secretion, increase insulin amount, increase the sensitvity of beta cell to insulin, inhibit and eliminate pathogenic factors and thus lower blood sugar and urine glucose. Animal experiment proves its no toxic side effect. clinical application shows that the biopreparation has obvious curative effect of lowering blood sugar and urine glucose as well as certain curative effect of lowering blood lipoid and blood viscosity.
Description
Technical field
The present invention relates to a kind of hypoglycemic biopreparation that utilizes the micropopulation development, said preparation is applicable to diabetics
Background technology
Diabetes are a kind of general chronic progressive diseases, are a kind of very complicated endocrine metabolism diseases.Its basic pathology is absoluteness or relative property hypoinsulinism, and the β cell reduces the sensitivity of insulin and endocrine disturbance's disease of causing, and then causes sugar, protein, fat and water, metabolic disturbance of electrolyte in the human body.Along with the easy concurrent pink man change of prolongation of time, serious threat health and life security.It is reported that up to the present the whole world has 300,000,000 people to suffer from diabetes approximately, wherein domestic just have more than 6,000 ten thousand people.
Current, the medicine of treatment diabetes is many, but great majority blood sugar lowering Western medicine and to keep on a diet be main adopts insulin injection in case of necessity.Western medicine biguanides, flavonoid medicine may command blood glucose, glucose in urine raises so that reach normal.Release Chinese medicine preparation treatment diabetes recently again, have " Chinese medicine live pancreas element ", " the blood sugar lowering pancreas is multiple red ", " JIANGTANGNING JIAONANG ", " sugared recovery capsule " etc. to be used for blood sugar lowering, glucose in urine etc.
In recent years, people begin to note utilizing biotechnology and microbial resources to extract its metabolite and make medicine in order to the treatment diabetes.For example: Chinese patent 99105621.3 " a kind of medicine for the treatment of diabetes " is exactly to utilize a strain microbial strains and other organic and inorganic chemicals to make BM oral liquid or the BM capsule medicine in order to the treatment diabetes for the metabolite extracting solution of culture medium fermentation.And for example: Beijing is great Chinese medicine preparation that " the novel diabetes preparation " that reach the development of scientific and technological development company be utilizes the metabolite of microbial solid fermentation to develop for main material is not the pure biological preparation of microbial metabolic products.The present invention extracts through the metabolite of the micropopulation of for many years experiment and research and utilization multiple beneficial and mixed culture fermentation thereof and has made hypoglycemic biopreparation and be used for treating diabetes.
Summary of the invention
The object of the invention is that the hypoglycemic biopreparation of developing is made up of multiple micropopulation and metabolite thereof, makes through methods such as the activation of routine, amplification culture, fermentation, filtration, slagging-off, degerming, lyophilizations.The comprehensive and systematic adjustment organism metabolism function of said preparation energy, the islet tissue of repairing pathological changes recovers its physiological function, promotes insulin secretion, increases insulin quantity and strengthens the sensitivity of β cell to insulin, suppress effectively and eliminate paathogenic factor, and then blood glucose, glucose in urine are reduced.Said preparation is checked and colibacillary testing identity through the animal toxicology of relevant health and epidemic prevention department: said preparation has no side effect, and escherichia coli all meet national standard.And take through the ordered series of numbers diabetics, its result proves that said preparation not only has obvious effects to blood sugar lowering, glucose in urine, and blood fat reducing and blood viscosity are also had certain curative effect.
Hypoglycemic biopreparation of the present invention, it is made up of multiple micropopulation and metabolite thereof, makes through methods such as the activation of routine, amplification culture, fermentation, filtration, slagging-off, degerming, lyophilizations; Multiple micropopulation in the said preparation is a saccharomycetic mixture commonly used on actinomycetes that can produce antibiotics in the antibacterial and Gram pharmaceutically commonly used lactobacillus that positive and negative are arranged and the fungus Chinese medicine; Metabolite is the metabolite that produces after the culture medium that micropopulation consumes.
The preparation method of hypoglycemic biopreparation is pressed routine step and is carried out:
A, at first mixed bacteria is activated, amplification culture, fermentation, fermentation process is the liquid submerged fermentation method, used culture medium is carbon source, nitrogenous source and inorganic salt in the fermentation, wherein carbon source is wheat bran or the Semen Maydis powder or the rice dregs of rice; Nitrogenous source is bean cake or soybean cake or the Semen arachidis hypogaeae dregs or the Semen Viciae fabae dregs of rice; Inorganic salt is sodium chloride or dipotassium hydrogen phosphate, carbon/nitrogen=10-30; The prescription of fermentation medium is the radix weight in grams for producing preparation with 250 liters of fermentation tanks: carbon source is the 2000-5000 gram, and nitrogenous source is the 100-300 gram, and inorganic salt is the 5-100 gram; Fermentation temperature 18-40 ℃, space changing 3-5 time, the fermentation pH value is 3.5-7.5, and the fermentation ventilation is volume ratio 1: 1, and the fermentation tank pressure is 0.01-0.08Mpa, and space changing 3 times, fermentation time are 25-90 hour;
B, fermentation liquid is promptly got biological blood sugar lowering revealing after the filtration of high speed centrifugation membrance separation, slagging-off, degerming, is that finished product gets final product through test package;
C, fermentation liquid is carried out lyophilization making powder after the high speed centrifugation membrance separation filters, again powder is made capsule or tablet with traditional method, is that finished product gets final product through test package.
Fermentation temperature space changing amplitude is 18-28 ℃ in the preparation method of hypoglycemic biopreparation, 28-33 ℃, and 33-40 ℃; Fermentation tank pressure conversion amplitude is 0.01-0.02Mpa, 0.02-0.04Mpa, 0.04-0.08Mpa.
Description of drawings
Fig. 1 is a process chart of the present invention
The specific embodiment
Embodiment 1 produces preparation with 250 liters of fermentation tanks
At first mixed bacteria is activated, amplification culture, fermentation, fermentation process is the liquid submerged fermentation method, used culture medium in the fermentation: carbon source wheat bran 2000 grams, nitrogenous source bean cake 100 grams, inorganic salt sodium chloride 5 grams, fermentation pH value 3.5, fermentation temperature 18-40 ℃, space changing 3 times, temperature is 18 ℃ for the first time, temperature is 28 ℃ for the second time, temperature is 38 ℃ for the third time, and the fermentation ventilation is volume ratio 1: 1, and the fermentation tank pressure is 0.01-0.08Mpa, fermentation time is 85 hours, and the total bacterium of product is measured 8,000,000,000/ml;
Fermentation liquid promptly being got biological blood sugar lowering again and reveal after the filtration of high speed centrifugation membrance separation, slagging-off, degerming, is that finished product gets final product through test package.
Embodiment 2 produces preparation with 250 liters of fermentation tanks
At first mixed bacteria is activated, amplification culture, fermentation, fermentation process is the liquid submerged fermentation method, used culture medium in the fermentation: carbon source Semen Maydis powder 3000 grams, nitrogenous source soybean cake 200 grams, inorganic salt dipotassium hydrogen phosphate 50 grams, fermentation pH value 5.5, fermentation temperature 18-40 ℃, space changing 5 times, temperature is 18 ℃ for the first time, and temperature is 23 ℃ for the second time, and temperature is 28 ℃ for the third time, 33 ℃ of the 4th temperature, 40 ℃ of the 5th temperature, the fermentation ventilation is volume ratio 1: 1, the fermentation tank pressure is 0.01-0.08Mpa, space changing 3 times, for the first time tank pressure 0.02Mpa, tank pressure 0.04Mpa, tank pressure 0.08Mpa for the third time for the second time, fermentation time is 30 hours, and the total bacterium of product is measured 9,000,000,000/ml;
Fermentation liquid being carried out lyophilization make powder after the high speed centrifugation membrance separation filters, again powder is made capsule or tablet with traditional method, is that finished product gets final product through test package.
Embodiment 3 produces preparation with 250 liters of fermentation tanks
At first mixed bacteria is activated, amplification culture, fermentation, fermentation process is the liquid submerged fermentation method, used culture medium in the fermentation: the carbon source rice dregs of rice 4000 grams, nitrogenous source Semen arachidis hypogaeae dregs 300 grams, fermentation pH value 7.5, fermentation temperature 18-40 ℃, space changing 3 times, temperature is 24 ℃ for the first time, temperature is 35 ℃ for the second time, temperature is 40 ℃ for the third time, and the fermentation ventilation is volume ratio 1: 1, and the fermentation tank pressure is 0.01-0.08Mpa, fermentation time is 25 hours, and the total bacterium of product is measured 7,000,000,000/ml;
Fermentation liquid promptly being got biological blood sugar lowering again and reveal after the filtration of high speed centrifugation membrance separation, slagging-off, degerming, is that finished product gets final product through test package;
Embodiment 4 produces preparation with 250 liters of fermentation tanks
At first mixed bacteria is activated, amplification culture, fermentation, fermentation process is the liquid submerged fermentation method, used culture medium in the fermentation: carbon source wheat bran 5000 grams, the nitrogenous source Semen Viciae fabae dregs of rice 300 grams, inorganic salt sodium chloride 100 grams, fermentation pH value 7.0, fermentation temperature 18-40 ℃, space changing 3 times, temperature is 20 ℃ for the first time, temperature is 30 ℃ for the second time, temperature is 38 ℃ for the third time, and the fermentation ventilation is volume ratio 1: 1, and the fermentation tank pressure is 0.01-0.08Mpa, space changing 3 times, for the first time tank pressure 0.01Mpa, tank pressure 0.02Mpa, tank pressure 0.04Mpa for the third time for the second time, fermentation time is 85 hours, and the total bacterium of product is measured 9,500,000,000/ml;
Again fermentation liquid being carried out lyophilization after the high speed centrifugation membrance separation filters and make powder, again powder is made capsule or tablet with traditional method, is that finished product gets final product through test package.
Claims (3)
1, a kind of hypoglycemic biopreparation is characterized in that it is made up of multiple micropopulation and metabolite thereof, makes through methods such as the activation of routine, amplification culture, fermentation, filtration, slagging-off, degerming, lyophilizations; Multiple micropopulation in the said preparation is a saccharomycetic mixture commonly used on actinomycetes that can produce antibiotics in the antibacterial and Gram pharmaceutically commonly used lactobacillus that positive and negative are arranged and the fungus Chinese medicine; Metabolite is the metabolite that produces after the culture medium that micropopulation consumes.
2, the preparation method of hypoglycemic biopreparation according to claim 1 is characterized in that pressing routine step and carries out:
A, at first mixed bacteria is activated, amplification culture, fermentation, fermentation process is the liquid submerged fermentation method, used culture medium is carbon source, nitrogenous source and inorganic salt in the fermentation, wherein carbon source is wheat bran or the Semen Maydis powder or the rice dregs of rice; Nitrogenous source is bean cake or soybean cake or the Semen arachidis hypogaeae dregs or the Semen Viciae fabae dregs of rice; Inorganic salt is sodium chloride or dipotassium hydrogen phosphate, carbon/nitrogen=10-30; The prescription of fermentation medium is a radix for restraining with 250 liters of fermentation tank production weight of formulation: carbon source is the 2000-5000 gram, and nitrogenous source is the 100-300 gram, and inorganic salt is the 5-100 gram; Fermentation temperature 18-40 ℃, space changing 3-5 time, the fermentation pH value is 3.5-7.5, and the fermentation ventilation is volume ratio 1: 1, and the fermentation tank pressure is 0.01-0.08Mpa, and space changing 3 times, fermentation time are 25-90 hour;
B, fermentation liquid is promptly got biological blood sugar lowering revealing after the filtration of high speed centrifugation membrance separation, slagging-off, degerming, is that finished product gets final product through test package;
C, fermentation liquid is carried out lyophilization making powder after the high speed centrifugation membrance separation filters, again powder is made capsule or tablet with traditional method, is that finished product gets final product through test package.
3, the preparation method of hypoglycemic biopreparation according to claim 2 is characterized in that fermentation temperature space changing amplitude is 18-28 ℃, 28-33 ℃, and 33-40 ℃; Fermentation tank pressure conversion amplitude is 0.01-0.02Mpa, 0.02-0.04Mpa, 0.04-0.08Mpa.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB011304502A CN1239166C (en) | 2001-11-16 | 2001-11-16 | Hypoglycemic biopreparation |
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| CNB011304502A CN1239166C (en) | 2001-11-16 | 2001-11-16 | Hypoglycemic biopreparation |
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| CN1357360A true CN1357360A (en) | 2002-07-10 |
| CN1239166C CN1239166C (en) | 2006-02-01 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011063597A1 (en) * | 2009-11-25 | 2011-06-03 | Wang Liping | Complex microbial preparation for treating diabetes and preparative method and use thereof |
| US8658156B2 (en) | 2007-07-05 | 2014-02-25 | Nestec S.A. | Supplemention of maternal diet |
| CN103783407A (en) * | 2014-01-08 | 2014-05-14 | 浙江工业大学 | Method for processing low-energy extruded meal replacement powder |
-
2001
- 2001-11-16 CN CNB011304502A patent/CN1239166C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8658156B2 (en) | 2007-07-05 | 2014-02-25 | Nestec S.A. | Supplemention of maternal diet |
| US9308228B2 (en) | 2007-07-05 | 2016-04-12 | Nestec S.A. | Supplemention of maternal diet |
| US9636369B2 (en) | 2007-07-05 | 2017-05-02 | Nestec S.A. | Supplemention of maternal diet |
| WO2011063597A1 (en) * | 2009-11-25 | 2011-06-03 | Wang Liping | Complex microbial preparation for treating diabetes and preparative method and use thereof |
| CN101703528B (en) * | 2009-11-25 | 2012-07-04 | 王立平 | Compound microbial preparation for treating diabetes, preparation method thereof and application thereof |
| CN103783407A (en) * | 2014-01-08 | 2014-05-14 | 浙江工业大学 | Method for processing low-energy extruded meal replacement powder |
| CN103783407B (en) * | 2014-01-08 | 2015-10-28 | 浙江工业大学 | A kind of processing method of low-yield extruding generation meal powder |
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