CN1349845A - Dialysis process and dialyzer for separating umbilical blood stem cell antifreezer - Google Patents
Dialysis process and dialyzer for separating umbilical blood stem cell antifreezer Download PDFInfo
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- CN1349845A CN1349845A CN 01113725 CN01113725A CN1349845A CN 1349845 A CN1349845 A CN 1349845A CN 01113725 CN01113725 CN 01113725 CN 01113725 A CN01113725 A CN 01113725A CN 1349845 A CN1349845 A CN 1349845A
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- stem cell
- sleeve
- antifreezer
- blood stem
- dialysis
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Abstract
The present invention relates to a dialysis method for separating umbilical blood stem cell antifreezer and its dialyzer equipment. It is characterized by that it adopts couuntercurrent dialysis method, i.e. feeds the umbilical blood into said equipment from one end, and feeds the dialyzate the equipment from side pipe hole of its other end inversely, and said equipment includes sleeve barrel and porous film fibre tube, 500-odd fibre tubes are placed in the steeve barrel along the axial direction. The adoption of said method and its equipment can remove 95% of DMSO within few min, and does not produce cell adhesion, and can effectively solve the problems of cell aggregation, cell loss, osmotis damage and bacterial pollution, etc. in centrifugal method, and can greatly raise its cell yield and functional activity.
Description
The present invention relates to dialysis process and the dialysis apparatus of a kind of separating umbilical blood stem cell antifreezer DMSO.
In hematopoietic stem cell (HPC) cryopreservation, must add contain antifreezing agent DMSO hypothermic protective solution to prevent the low temperature injury of cell.Yet,, therefore, also the DMSO in the cytoprotective liquid must be taken out behind the rewarming and could import human body because DMSO is toxic to human body under room temperature and/or body temperature.At present, generally be to adopt centrifuging to separate DMSO.But mechanical force and the osmotic pressure that is produced by high speed rotating in the method can cause cell aggregation agglomerating and make a large amount of cells impaired; In addition, because centrifugal separating device is not a closed system, thereby this method and install operational non-tight, also can make navel blood stem cell be subjected to germ contamination.
The objective of the invention is to:, provide a kind of novel diffusion type to clean the dialysis process and the dialysis apparatus of separating umbilical blood stem cell antifreezer for overcoming above-mentioned the deficiencies in the prior art part.
Its goal of the invention realizes by following technical solution.
The dialysis process of separating umbilical blood stem cell antifreezer is characterized in that:
A, the Cord blood that will add hypothermic protective solution are sent into dialysis apparatus from the telescopic port of dialysis apparatus, and flow out from the sleeve another port,
B, with the side mouth of pipe reverse the pump into dialysis apparatus of dialysis solution from dialysis apparatus sleeve Cord blood outflow end.
A kind of dialysis apparatus that uses the dialysis process of the described separating umbilical blood stem cell antifreezer of claim 1 is characterized in that: comprise sleeve and porous membrane fiber pipe; Sleeve is a cylinder barrel shaped, both ends open, and its side, two ends is respectively equipped with the mouth of pipe; Fiber pipe more than 500 is located in the sleeve vertically.
End, described sleeve two ends, the gap location between fiber pipe and fiber pipe and fiber pipe and the sleeve lining are provided with the solid piece piece of shutoff.
Described telescopic internal diameter is 4～10mm, and length is 4～10cm, is a miniature socket.
Described sleeve can be made as glass, lucite or rustless steel etc. by the rigid material nontoxic, harmless to human body.
Its internal diameter of described fiber pipe is 100～200 μ m, pipe thickness 15～30 μ m, and tube wall is a perforated membrane.
Described fiber pipe tubing is the cellulose triacetate film of voidage 70%.
Dialysis apparatus of the present invention is reasonable in design, and is simple in structure, adopts dialysis process of the present invention and dialysis apparatus, can be in minutes 95% DMSO in the Cord blood be removed, and in operating process the cytoadherence phenomenon do not take place.Solved the cell aggregation in the centrifuging, cell loss, infiltration damage and all problems of germ contamination effectively, made the response rate of cell and functional activity thereof that raising by a relatively large margin arranged.
Fig. 1 is this utility model structural representation,
Fig. 2 is the A-A cutaway view of Fig. 1.
Below in conjunction with accompanying drawing, the present invention is further described.
Embodiment:
The dialysis apparatus of this separating umbilical blood stem cell antifreezer comprises sleeve 1 and porous membrane fiber pipe 2.
Sleeve 1 is a cylinder barrel shaped, available nontoxic, the harmless rigid material of human body such as glass, lucite, rustless steel etc. is made.The internal diameter of sleeve 1 is 6mm, and length is 8cm, and its both ends open flows to for cell suspending liquid and flows out; Its side, two ends has the mouth of pipe 3, and confession dialysis solution (abluent) flows to and flows out; 500 porous membrane fiber pipes are contained in the sleeve 1 vertically.
End, described sleeve 1 two ends, shutoff is carried out with the solid piece piece 5 of the material formation of silicone rubber and so in gap between porous membrane fiber pipe 2 and porous membrane fiber pipe 2 and porous membrane fiber pipe 2 and sleeve 1 inwall, so that dialysis solution does not mix mutually with cell suspending liquid, guarantee that blood flows through from porous membrane fiber pipe 2, and dialysis solution can only flow between porous membrane fiber pipe 2 tube bundles.Suit the end cap 4 of fenestrated on the two-port of sleeve 1.
Described porous membrane fiber pipe is the cellulose triacetate film pipe, and its internal diameter is 150 μ m, pipe thickness 20 μ m, and micropore is arranged on the tube wall, and the size in its aperture only can be held the DMSO molecule and passes through, and its voidage is 70%.
Operating process during it uses is such:
The Cord blood sample that has added hypothermic protective solution is lowered the temperature with special heat sink, place liquid nitrogen to preserve then.After date in the time of one section uses as need, again with the Cord blood rewarming.
At first, send the navel blood stem cell suspension (blood) that contains DMSO behind the rewarming to dialysis apparatus from an end of dialysis apparatus sleeve 1, and make in its porous membrane fiber pipe 2 in sleeve 1 and to flow, and flow out from the another port.
Then, the lateral mouth of pipe 3 of dialysis solution from dialysis apparatus sleeve 1 Cord blood outflow end pumped into, and make edge and blood rightabout mobile (adverse current dialysis) between its slit outside porous membrane fiber pipe 2 under the effect of pump.DMSO in the porous membrane fiber pipe 2 is diffused in the dialysis solution by cell suspending liquid gradually by the porous membrane tube wall.Utilize pump to make cell suspending liquid, from cell suspending liquid, dispose the clinical use of being allowed for access up to most of DMSO circularly by dialyser (closed system).
Claims (6)
1, the dialysis process of separating umbilical blood stem cell antifreezer is characterized in that:
A, the Cord blood that will add hypothermic protective solution are sent into dialysis apparatus from the telescopic port of dialysis apparatus, and flow out from the sleeve another port,
B, with the side mouth of pipe reverse the pump into dialysis apparatus of dialysis solution from dialysis apparatus sleeve Cord blood outflow end.
2, a kind of dialysis apparatus that uses the dialysis process of the described separating umbilical blood stem cell antifreezer of claim 1 is characterized in that: comprise sleeve and porous membrane fiber pipe; Sleeve is a cylinder barrel shaped, both ends open, and its side, two ends is respectively equipped with the mouth of pipe; Fiber pipe more than 500 is located in the sleeve vertically.
3, the dialyser of separating umbilical blood stem cell antifreezer according to claim 2 is characterized in that: end, described sleeve two ends, the gap location between fiber pipe and fiber pipe and fiber pipe and the sleeve lining are provided with the solid piece piece of shutoff.
4, the dialyser of separating umbilical blood stem cell antifreezer according to claim 2 is characterized in that: described telescopic internal diameter is 4～10mm, and length is 4～10cm, is a miniature socket.
5, the dialyser of separating umbilical blood stem cell antifreezer according to claim 2 is characterized in that: its internal diameter of described fiber pipe is 100～200 μ m, pipe thickness 15～30 μ m, and tube wall is a perforated membrane.
6, the dialyser of separating umbilical blood stem cell antifreezer according to claim 5 is characterized in that: described fiber pipe tubing is the cellulose triacetate film of voidage 70%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01113725 CN1349845A (en) | 2001-06-22 | 2001-06-22 | Dialysis process and dialyzer for separating umbilical blood stem cell antifreezer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01113725 CN1349845A (en) | 2001-06-22 | 2001-06-22 | Dialysis process and dialyzer for separating umbilical blood stem cell antifreezer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1349845A true CN1349845A (en) | 2002-05-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01113725 Pending CN1349845A (en) | 2001-06-22 | 2001-06-22 | Dialysis process and dialyzer for separating umbilical blood stem cell antifreezer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1349845A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101505811B (en) * | 2006-08-24 | 2012-05-23 | 弗雷泽纽斯医疗保健控股有限公司 | Device for removing fluid from blood in a patient |
| CN102036688B (en) * | 2007-10-05 | 2014-07-02 | 伊西康公司 | Repair and regeneration of renal tissue using human umbilical cord tissue-derived cells |
| CN105994250A (en) * | 2016-05-19 | 2016-10-12 | 电子科技大学 | Method for adding or removing low-temperature protective agent for cells on basis of microfluidic and membrane separation techniques |
-
2001
- 2001-06-22 CN CN 01113725 patent/CN1349845A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101505811B (en) * | 2006-08-24 | 2012-05-23 | 弗雷泽纽斯医疗保健控股有限公司 | Device for removing fluid from blood in a patient |
| CN102036688B (en) * | 2007-10-05 | 2014-07-02 | 伊西康公司 | Repair and regeneration of renal tissue using human umbilical cord tissue-derived cells |
| CN105994250A (en) * | 2016-05-19 | 2016-10-12 | 电子科技大学 | Method for adding or removing low-temperature protective agent for cells on basis of microfluidic and membrane separation techniques |
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |