CN1348992A - Continuous single-tank fermentation process of bacillus in membrane coupled clear liquid for producing levolactic acid - Google Patents
Continuous single-tank fermentation process of bacillus in membrane coupled clear liquid for producing levolactic acid Download PDFInfo
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- CN1348992A CN1348992A CN 01134135 CN01134135A CN1348992A CN 1348992 A CN1348992 A CN 1348992A CN 01134135 CN01134135 CN 01134135 CN 01134135 A CN01134135 A CN 01134135A CN 1348992 A CN1348992 A CN 1348992A
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Abstract
This invention is a technology for producing levo-monolactic acid by coupling together the membrane separation technique with clear solution single-tank bacterial continuous fermentation. The said technological method is as follows: (a). corn is non-acid-soaked and crushed, and has embryonic bud removed; (b). corn embryonic milk is ground into pulp, and then it is liquefied, saccharificated and filtered to separate saccharide liquor; (c). the saccharide liquor after being concentrated is added with other nutritive sources fermented by lactic acid together into an anae robic fermentation tank for bacterial continuous fermentation; (d). after the fermented liquor is micro-membrane-filtered for initial conentraiton; and (e). the fermented liquor is further heated for concentration and vacuum-rectification to obtain the levo-monolactic acid.
Description
One, technical field
The present invention is a kind of Technology of (left-handed)-lactic acid-producing, especially a kind of membrane separation technique and clear liquor in single tank bacterium are continuously fermented to be coupled together produce the technology of L-lactic acid.
Two, background technology
The Rhizopus oryzae fermentation method is mostly adopted in the production of L-lactic acid both at home and abroad at present, during the fermentation, because the accumulation of lactic acid has seriously suppressed the growth of milk-acid bacteria and the formation of lactic acid.In order to eliminate this restraining effect, often add some neutralizing agents, as CaCO
3, NaOH, NH
4OH.Yet these methods all have some shortcomings, for example add excess amount of Ca CO
3Control the pH value of fermented liquid, fermentor tank has been gone in a large amount of impurity and assorted cingula extracted for fermentation and back all to have brought very big trouble, in addition, the fermentation of employing Rhizopus oryzae is an aerobic fermentation, the energy consumption height, make product cost high, all restricting L-lactic acid and promoting the use and develop.Especially current; in order to protect our survival environment, just wideling popularize poly(lactic acid) novel degradable material both at home and abroad, how to realize high-quality; low cost, free of contamination lactic acid-producing have become the key problem in technology that can restriction poly(lactic acid) novel environment friendly novel material smooth implementation.
Three, summary of the invention:
1, goal of the invention: the purpose of this invention is to provide a kind of raw material comprehensive utilization ratio height, the glucose acid invert ratio height, the product purity height, production technique is simple, cost is low, and no waste is the membrane coupled clear liquor in single tank bacterium of the non-wastewater discharge left-handed-lactic acid-producing technology of continuously fermenting also.
2, technical scheme: left-handed-lactic acid-producing technology that membrane coupled clear liquor in single tank bacterium of the present invention is continuously fermented is:
A, corn there is not acid dipping, and broken corn, isolate plumule;
B, the corn embryosperm defibrination that will peel off plumule, liquefaction, saccharification, filter to isolate liquid glucose;
C, the liquid glucose that filters to isolate is concentrated the back and lactic acid fermented other nutrition source adds anaerobic fermentation tank together, carry out bacterium and continuously ferment;
D, on fermented liquid carry out micro-filtrate membrane filtration, carry out nanofiltration membrane afterwards again and filter, will be tentatively concentrated through above filtering fermented liquid;
E, will concentrate rectification under vacuum obtains left-handed-lactic acid through preliminary spissated fermented liquid again through heat.
Plumule and endosperm in the corn after adopting swirler with fragmentation are isolated.
Adopt the method for vacuum filtration, will isolate liquid glucose through the maize serum of liquefaction, saccharification.It is that membrane coupled clear liquor in single tank continuously ferments that the bacterium that carries out in anaerobic fermentation tank is continuously fermented, and fermenting bacteria is a milk-acid bacteria.
Carry out tentatively concentrated through reverse osmosis membrane through the filtering fermented liquid of nanofiltration membrane.
Through preliminary spissated fermented liquid when concentrating, it is concentrated to adopt the triple-effect evaporator of band heat pump to carry out heat, employing high vacuum rectification unit makes pharmaceutical grade during rectifying left-handed-lactic acid.
3, technique effect:
The invention has the advantages that
(1) raw material corn comprehensive utilization ratio height of the present invention, not only the inversion rate of glucose of main component starch can reach more than 97% of theoretical value, and non-starch component protein, fat and fiber have all obtained maximum utilization, byproduct reclaims the value of being created can offset more than 50% of lactic raw material cost, has significantly reduced the production cost of L-lactic acid.
Raw material pre-treating technology of the present invention is that the useful composition of each except that starch in the corn was promptly separated, extracts, processes, is refined into various high value added products before fermentation, and starch is converted into glucose enters the workshop section of fermenting.Improve fermentation workshop section usage ratio of equipment and do not adopt the sulfurous acid impregnation technology, avoided the waste water of sulfurous acid and its esters to produce in this stage.Enzymolysis three phase separation combination technique has not only improved albumen, fat and fiber recovery among the present invention, and has significantly improved proteinic quality, improves its nutrition balance, is a kind of albumen small peptide goods of high added value.
(2) the present invention adopts clear liquor in single tank bacterium anaerobism to continuously ferment, the lactic acid that fermentation generates by nanofiltration membrane saturating go out.Overcome milk-acid bacteria effectively and produced sour inhibition, reaction product has been reduced, the glucose acid invert ratio of its fermentation has increased substantially, and can reach about 95% of theoretical value, and the also more traditional batch fermentation of ferment strength exceeds more than 5 times.Reduce production costs to improving the quality of products, significant.
(3) extraction process adopts membrane separation technique behind the present invention, high-efficiency evaporating concentration technique and high vacuum rectification technology, can be described as modern physics refine technology, not only technical process is short, the extraction yield height, good product quality, energy consumption is low, and has reduced acid, alkali, activated carbon, tree significantly and consumption of auxiliary materials such as refer to.The cost that reduces product is played the important and pivotal role.
(4) technological process of the present invention has realized loop production, has accomplished wastewater zero discharge, in the former lactic acid-producing technological process discharge of wastewater is arranged not only, and also has a large amount of waste residue CaSO
4Discharge, the present invention has removed from office calcium salt method, does not therefore also have waste residue CaSO
4Discharging.Protect ecotope, saved waste water, required great amount of investment and the working cost of waste residue processing.Also play a role to reducing product cost.
Four, embodiment:
The embodiment of the invention is:
(1) corn is not had acid dipping, plumule is fully absorbed water;
(2) corn after the dipping back adopts swirler with fragmentation is isolated plumule, again plumule is made Semen Maydis oil with enzymolysis three phase separation combination technique, food protein small peptide and fiber feedstuff;
(3) endosperm defibrination, liquefaction, saccharification, the isolated by vacuum filtration that will peel off behind the plumule goes out liquid glucose, and the filter cake drying is made the scleroproein feed;
(4) with the liquid glucose that obtains after the vacuum filtration, after concentrating, be added to anaerobic fermentation tank with other nutrition source of lactic fermentation stream together, carry out the continuous fermentation using bacteria of membrane coupled clear liquor in single tank, produce L-lactic acid.
(5) sophisticated fermented liquid is pumped into microfiltration membrane, returning fermentor tank by the isolated bacterium circulation of microfiltration membrane utilizes again, the fermented liquid that is appeared by microfiltration membrane directly pumps into nanofiltration membrane, the nutritive substance of failing to be utilized that is come out by nanofiltration membrane separation also circulates and returns fermentor tank and utilize, the rare lactic acid that appears through the nanofiltration membrane purifying is pumped to reverse osmosis membrane and carries out tentatively concentrated, the process water that reverse osmosis membrane appears utilizes as the process water retrieval system again.
(6) after the preliminary spissated lactic acid of reverse osmosis membrane is undertaken by the triple effect falling-film evaporator of being with heat pump, concentrate, make water white transparency pharmaceutical grade L-lactic acid through the high vacuum rectification unit again.
Organic impuritys such as the albumen that obtains in the refinery practice, polysaccharide, pigment are added to raw material pre-treatment worker and praise in the section fibre protein fodder and utilize.
The concentration that wherein flows sugaring is greater than 60%, 50～60 ℃ of fermentating liquid PH values of leavening temperature are 2.0～4.0, produce acid 〉=9.0%, transformation efficiency 90～95%, residual sugar is less than 0.3% in the fermentation liquid, extract total recovery greater than 85% with the corn embryosperm defibrination, behind liquefaction, saccharification, the separating glucose, the method of filter cake being made protein fodder and fiber feedstuff is that filter cake is sized mixing, and separates, promptly gets protein fodder and fiber feedstuff after dehydration and the drying.
By the plumule producd fibers feed after peeling off, the method that Semen Maydis oil, food protein small peptide are paid product is earlier that plumule is freezing, and defibrination, enzymolysis obtain fiber feedstuff again behind three phase separation and concentrate drying then, food protein small peptide and Semen Maydis oil.
Wherein freezing temp is-5 ℃～-25 ℃, and freezing time is 20～200 minutes, and hydrolysis temperature is 50 ℃～60 ℃, and enzymolysis time is 10～20 hours.The yield of Semen Maydis oil reaches about 85%.
Calculate to produce 10,000 pharmaceutical grade L-lactic acid year:
Consume 18000 tons of corns, produce 10,000 tons of pharmaceutical grade L-lactic acid, 300 tons in food protein small peptide powder, 450 tons of Semen Maydis oils, 700 tons of protein fodders, 2000 tons of fiber feedstuffs.Lactic acid cost per ton can drop to below 10,000 yuan.
Claims (6)
1, left-handed-lactic acid-producing technology that a kind of membrane coupled clear liquor in single tank bacterium is continuously fermented is characterized in that production technique is:
A, corn there is not acid dipping, and broken corn, isolate plumule;
B, the corn embryosperm defibrination that will peel off plumule, liquefaction, saccharification, filter to isolate liquid glucose;
C, the liquid glucose that filters to isolate is concentrated the back and lactic acid fermented other nutrition source adds anaerobic fermentation tank together, carry out bacterium and continuously ferment;
D, above fermented liquid is carried out micro-filtrate membrane filtration, carry out nanofiltration membrane afterwards again and filter, will be tentatively concentrated through above filtering fermented liquid;
E, will concentrate rectification under vacuum obtains left-handed-lactic acid through preliminary spissated fermented liquid again through heat.
2, left-handed-lactic acid-producing technology that membrane coupled clear liquor in single tank bacterium according to claim 1 is continuously fermented, plumule and endosperm in the corn after it is characterized in that adopting swirler with fragmentation are isolated.
3, left-handed-lactic acid-producing technology that membrane coupled clear liquor in single tank bacterium according to claim 1 and 2 is continuously fermented is characterized in that adopting the method for vacuum filtration, will isolate liquid glucose through the maize serum of liquefaction, saccharification.
4, left-handed-lactic acid-producing technology that membrane coupled clear liquor in single tank bacterium according to claim 1 is continuously fermented is characterized in that it is that membrane coupled clear liquor in single tank continuously ferments that the bacterium that carries out is continuously fermented in anaerobic fermentation tank, fermenting bacteria is a milk-acid bacteria.
5, left-handed-lactic acid-producing technology that membrane coupled clear liquor in single tank bacterium according to claim 1 is continuously fermented is characterized in that carrying out tentatively concentrated through reverse osmosis membrane through the filtering fermented liquid of nanofiltration membrane.
6, left-handed-lactic acid-producing technology that membrane coupled clear liquor in single tank bacterium according to claim 1 is continuously fermented, when it is characterized in that the preliminary spissated fermented liquid of process concentrates, adopt the triple-effect evaporator of band heat pump to carry out heat and concentrate, employing high vacuum rectification unit makes pharmaceutical grade during rectifying left-handed-lactic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01134135 CN1219068C (en) | 2001-10-31 | 2001-10-31 | Continuous single-tank fermentation process of bacillus in membrane coupled clear liquid for producing levolactic acid |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01134135 CN1219068C (en) | 2001-10-31 | 2001-10-31 | Continuous single-tank fermentation process of bacillus in membrane coupled clear liquid for producing levolactic acid |
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| Publication Number | Publication Date |
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| CN1348992A true CN1348992A (en) | 2002-05-15 |
| CN1219068C CN1219068C (en) | 2005-09-14 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102659564A (en) * | 2012-05-17 | 2012-09-12 | 安徽中粮生化格拉特乳酸有限公司 | Method for extracting lactic acid from wastewater generated in lactic acid production |
| CN104919051A (en) * | 2012-12-28 | 2015-09-16 | 艾尼股份公司 | Process for the production of lipids from biomass |
| CN106589327A (en) * | 2016-12-11 | 2017-04-26 | 闫博文 | Polylactic acid and preparation method thereof |
| CN115581798A (en) * | 2022-11-01 | 2023-01-10 | 珠海市雅莎医疗器械有限公司 | Liquid wound dressing for improving dark skin color and preparation method thereof |
-
2001
- 2001-10-31 CN CN 01134135 patent/CN1219068C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102659564A (en) * | 2012-05-17 | 2012-09-12 | 安徽中粮生化格拉特乳酸有限公司 | Method for extracting lactic acid from wastewater generated in lactic acid production |
| CN104919051A (en) * | 2012-12-28 | 2015-09-16 | 艾尼股份公司 | Process for the production of lipids from biomass |
| CN104919051B (en) * | 2012-12-28 | 2018-10-30 | 艾尼股份公司 | Technique for producing lipid from biomass |
| CN106589327A (en) * | 2016-12-11 | 2017-04-26 | 闫博文 | Polylactic acid and preparation method thereof |
| CN115581798A (en) * | 2022-11-01 | 2023-01-10 | 珠海市雅莎医疗器械有限公司 | Liquid wound dressing for improving dark skin color and preparation method thereof |
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| Publication number | Publication date |
|---|---|
| CN1219068C (en) | 2005-09-14 |
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