CN1343514A

CN1343514A - Chinese medicine for treating fatty liver

Info

Publication number: CN1343514A
Application number: CN 01127118
Authority: CN
Inventors: 吴翠贞; 王瑞云; 戚晓红
Original assignee: Nanjing Medical University
Current assignee: Nanjing University; Nanjing Medical University
Priority date: 2001-08-17
Filing date: 2001-08-17
Publication date: 2002-04-10
Anticipated expiration: 2021-08-17
Also published as: CN1131060C

Abstract

A composite Chinese medicine for treating fatty liver is prepared from Chitosan and 5 Chinese-medicinal materials includign prepared fleece flower root, red sage root, achyranthes root, etc. through proportional mixing. It features high curative effect.

Description

The compound Chinese medicinal preparation of treatment fatty liver

One, technical field

The invention belongs to the compound Chinese medicinal preparation field.

Two, background technology

Fatty liver is modal clinically DHD, and its sickness rate accounts for 10% of general population's sum, near the sickness rate of hepatitis B, and the trend that raises is year by year arranged.Fatty liver such as untimely diagnosis and treatment, some cases can develop into liver cirrhosis, even the serious consequence of liver failure.

At present, still there is not ideal specific treatment medicine for fatty liver both at home and abroad.Herb Gynostemmae Pentaphylli total glycosides commonly used clinically, Max EPA, clofibrate, Leping fat etc., big multiaction relaxes, and curative effect is not remarkable, and the medicine life-time service that has has damage to liver, should not be used for the treatment of fatty liver, and the medicine that has also can produce toxic and side effects.

Three, summary of the invention

For seek a kind of safe, liver is had no side effect and medicine evident in efficacy, we are through research for many years, the compound preparation that filters out Six-element Chinese medicine preparation is treated fatty liver, finds that it is evident in efficacy.This Six-element Chinese medicine is: chitosan, Radix Polygoni Multiflori Preparata, Radix Salviae Miltiorrhizae, Radix Achyranthis Bidentatae, Rhizoma Alismatis and Semen Cassiae.

Chitosan is a kind of medicine that effectively reduces cholesterolemia (TC) and triglyceride (TG), and this effect may be that chitosan stops digestive system to absorb TG and the event of TC.Radix Polygoni Multiflori Preparata has the nourishing the liver hepatoprotective, the effect of liver and kidney tonifying, and it can reduce the absorption of intestinal to cholesterol, promotes cholesterol to drain, and wherein Feng Fu vegetable lecithin can also stop cholesterol at intrahepatic deposition, is of value to the treatment of fatty liver.Radix Salviae Miltiorrhizae has activating blood circulation to dissipate blood stasis, improves liver microcirculation, increases the supply of hepatocyte nutrition and oxygen, promotes the oxidation of liver inner lipid and has the effect of liver lipid lowering.Contain the oleanolic acid that produces after the triterpenoid saponin hydrolysis in the Radix Achyranthis Bidentatae, have protect the liver, anti-liver fat change effect.Rhizoma Alismatis can suppress exogenous TG, TC and absorb, and suppresses the synthetic intrahepatic fat content that makes of the interior TG of liver and reduces.Semen Cassiae can reduce blood TG and TC.Because the not same-action of these medicines produces complementary, treatment has significant curative effect to fatty liver and make its compound preparation.Similarly prescription does not appear in the newspapers as yet.

The compound Chinese medicinal preparation of treatment fatty liver of the present invention is pressed 30～40%, 10～20%, 10～20%, 10～20%, 10～20%, 10～20% mixed by chitosan, Radix Polygoni Multiflori Preparata, Radix Salviae Miltiorrhizae, Radix Achyranthis Bidentatae, Rhizoma Alismatis and Semen Cassiae, behind the decocting in water precipitate with ethanol, spray drying is the fine-powdered crude drug, can be made into capsule, tablet etc.

With this compound Chinese medicinal preparation rat is carried out therapeutic test:

1, rat fat liver Preparation of model

1) feedstuff preparation: normal diet+20% Adeps Sus domestica+0.5% cholesterol+0.2% sodium cholate

2) model preparation: rat gave 40%CCl earlier on the same day of feeding high fat diet ₄Oleum Brassicae campestris solution 0.3ml/100g hind leg subcutaneous injection is fed to high lipid food again, promptly forms fatty liver after about six weeks.

2, test method

After rat forms fatty liver, with above-mentioned compound preparation 0.7g/kg/ days to rat oral gavage, once a day, continuous 8 weeks.DONGBAO GANTAI is as positive control, and being converted into the used dosage of rat by clinical adult's consumption is 0.7g/kg/ days, gives rat oral gavage, once a day.

3, result of the test

(1) compound Chinese medicinal preparation is to rats'liver TG, TC content influence

By Fig. 1,2 as seen, after rat was taken medicine, liver homogenate TG, TC content all obviously descended, and compare significant difference with model group.DONGBAO GANTAI treatment of control group effect is obviously not as the treatment group.

(2) compound Chinese medicinal preparation is to the active influence of rat serum glutamate pyruvate transaminase (ALT)

As seen from Figure 3, the active normal matched group of model group rat blood serum ALT significantly increases, and that treatment group serum ALT activities descends than model group is nearly 50%, illustrate that medicine damages rats'liver obvious protective effect is arranged.The DONGBAO GANTAI group is fallen the active effect of ALT not as the treatment group.

(3) pathological examination

1. perusal:

The model group rat liver obviously increases, the complexion changed Huang, and that touches has a greasy feeling, and tangent plane is graniphyric.Medication therapy groups rat liver color is more ruddy, and quality is near normal liver tissue.

2. light microscopy checking:

Rats in normal control group liver light microscopic shows no abnormal down, has the tiny fat of minute quantity to drip in rarely seen indivedual hepatocyte in Sudan IV dyeing and intersperses among around the nucleus, and sinus hepaticus is high-visible, liver rope marshalling (Fig. 5,6).To severe diffusivity steatosis, fat became hepatocyte and increases during the model group rat liver occurred, and the sinus hepaticus pressurized narrows down even disappears, liver rope arrangement disorder, performances such as the still visible hepatocyte edema, some lamellar necrosis and the cell infiltration that have.In paraffin section, fat is dissolved by ethanol, dimethylbenzene equal solvent, shows as cavity shape (Fig. 7); Fat drips and is then dyed Chinese red (Fig. 8) in frozen section, Sudan IV dyeing.Model group rats'liver fat range degree is the most serious, the apparition of treatment group fat alleviates, show as fat change hepatocyte number and obviously reduce, the tiny fat of rarely seen minority drips to be dispersed in and is distributed in the lobules of liver periphery cell, almost completely approaches normal liver tissue Pathologic specimen (Fig. 9,10).DONGBAO GANTAI group (Figure 11) is compared with model group (Fig. 7,8), and fat range degree has improvement, but effect is not as the treatment group.

3. graphical analysis:

Utilize the painted pathological section of Sudan IV on the computer color vision analytical system, to carry out the mensuration of absolute and relative area, the result is as shown in Figure 4: normal group only has a small amount of tiny fat to drip in the individual cells, and model group fat variable area increases greatly, and treatment group fat variable area approaches normal liver tissue substantially.DONGBAO GANTAI group effect is not as the treatment group.

(4) compound Chinese medicinal preparation is to the influence of rat blood serum and liver fat acid (FFA):

By Figure 12,13 as seen: model group rat blood serum and liver F FA content obviously increase, and compare with normal group that there were significant differences.The FFA level of treatment group serum and liver obviously descends, and compares significant difference with model group.DONGBAO GANTAI group therapeutic effect is obviously not as the treatment group.

(5) compound Chinese medicinal preparation is to the influence of rat blood serum endotoxin content:

As seen from Figure 14: fatty liver model group rat blood serum endotoxin significantly raises than normal group content, and treatment group serum endotoxin content obviously descends, and has compared significant difference with model group.DONGBAO GANTAI group endotoxin content descends to some extent, does not have significance but compare difference with model group.

(6) compound Chinese medicinal preparation is to the influence of rat blood serum tumor necrosis factor (TNF) content:

As seen from Figure 15: model group rat blood serum TNF concentration obviously raises than normal group, can reach about 15 times of normal group.Treatment group rat serum TNF content obviously drops near normal level, has compared significant difference with model group.DONGBAO GANTAI group TNF content descends to some extent, but effect is not as medication therapy groups.

Four, description of drawings

Fig. 1 compound Chinese medicinal preparation is to the influence of rats'liver TG.C: normal control group; M: fatty liver model group; T: treatment group; D: DONGBAO GANTAI matched group.Compare with the normal control group: Δ P＜0.05, Δ Δ P＜0.01, Δ Δ Δ P＜0.001; Compare with model group: ^*P＜0.05, ^*P＜0.01, ^{* *}P＜0.001.

Fig. 2 compound Chinese medicinal preparation is to the influence of rats'liver TC.C: normal control group; M: fatty liver model group; T: treatment group; D: DONGBAO GANTAI matched group.Compare with the normal control group: Δ P＜0.05, Δ Δ P＜0.01, Δ Δ Δ P＜0.001; Compare with model group: ^*P＜0.05, ^*P＜0.01, ^{* *}P＜0.001.

Fig. 3 compound Chinese medicinal preparation is to the influence of rat blood serum ALT.C: normal control group; M: fatty liver model group; T: treatment group; D: DONGBAO GANTAI matched group.Compare with the normal control group: Δ P＜0.05, Δ Δ P＜0.01, Δ Δ Δ P＜0.001; Compare with model group: ^*P＜0.05, ^*P＜0.01, ^{* *}P＜0.001.

Fig. 4 compound Chinese medicinal preparation is to the influence of rats'liver fat variable area.C: normal control group; M: fatty liver model group; T: treatment group; D: DONGBAO GANTAI matched group.Compare with the normal control group: Δ P＜0.05, Δ Δ P＜0.01, Δ Δ Δ P＜0.001; Compare with model group: ^*P＜0.05, ^*P＜0.01, ^{* *}P＜0.001.

Fig. 5 normal rats liver is no abnormal, and sinus hepaticus is high-visible, liver rope marshalling.

(HE×660)

Fig. 6 normal rats liver has the tiny fat of minute quantity to drip in only visible indivedual hepatocyte in Sudan IV dyeing and intersperses among around the nucleus.(Sudan IV * 660)

The diffusivity fat that varying degree appears in Fig. 7 model group rat liver becomes, and shows as the cavity that differs in size.(HE×330)

Fig. 8 model group rat liver has the fat that is dyed in a large number for Chinese red to drip in Sudan IV dyeing, and is the most serious around the lobule.(Sudan IV * 330)

Fig. 9 treatment group liver structure is near normal.(HE×660)

Figure 10 treatment group fat range degree is light, near the normal control group.(Sudan IV * 660)

Figure 11 DONGBAO GANTAI matched group, fat range degree has improvement, but effect is not as the treatment group.

(Sudan IV * 660)

Figure 12 compound Chinese medicinal preparation is to the influence of rat blood serum FFA.C: normal control group; M: fatty liver model group; T: treatment group; D: DONGBAO GANTAI matched group.Compare with the normal control group: Δ P＜0.05, Δ Δ P＜0.01, Δ Δ Δ P＜0.001; Compare with model group: ^*P＜0.05, ^*P＜0.01, ^{* *}P＜0.001.

Figure 13 compound Chinese medicinal preparation is to the influence of rats'liver FFA.C: normal control group; M: fatty liver model group; T: treatment group; D: DONGBAO GANTAI matched group.Compare with the normal control group: Δ P＜0.05, Δ Δ P＜0.01, Δ Δ Δ P＜0.001; Compare with model group: ^*P＜0.05, ^*P＜0.01, ^{* *}P＜0.001.

Figure 14 compound Chinese medicinal preparation is to the endotoxic influence of rat blood serum.C: normal control group; M: fatty liver model group; T: treatment group; D: DONGBAO GANTAI matched group.Compare with the normal control group: Δ P＜0.05, Δ Δ P＜0.01, Δ Δ Δ P＜0.001; Compare with model group: ^*P＜0.05, ^*P＜0.01, ^{* *}P＜0.001.

Figure 15 compound Chinese medicinal preparation is to the influence of rat blood serum TNF.C: normal control group; M: fatty liver model group; T: treatment group; D: DONGBAO GANTAI matched group.Compare with the normal control group: Δ P＜0.05, Δ Δ P＜0.01, Δ Δ Δ P＜0.001; Compare with model group: ^*P＜0.05, ^*P＜0.01, ^{* *}P＜0.001.

Claims

1. compound Chinese medicinal preparation for the treatment of fatty liver, it is characterized in that this compound preparation by chitosan, Radix Polygoni Multiflori Preparata, Radix Salviae Miltiorrhizae, Radix Achyranthis Bidentatae, Rhizoma Alismatis and six kinds of Chinese medicines of Semen Cassiae by 30～40%, 10～20%, 10～20%, 10～20%, 10～20%, 10～20% mixed, through the decocting in water precipitate with ethanol, spray drying forms.