CN1331609C - Quantitative liquid extraction device - Google Patents

Quantitative liquid extraction device Download PDF

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Publication number
CN1331609C
CN1331609C CNB2004100428788A CN200410042878A CN1331609C CN 1331609 C CN1331609 C CN 1331609C CN B2004100428788 A CNB2004100428788 A CN B2004100428788A CN 200410042878 A CN200410042878 A CN 200410042878A CN 1331609 C CN1331609 C CN 1331609C
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CN
China
Prior art keywords
piston
syringe needle
liquid
needle head
connecting rod
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CNB2004100428788A
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Chinese (zh)
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CN1704168A (en
Inventor
张铁中
徐赫
徐喆
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China Agricultural University
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China Agricultural University
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Publication date
Application filed by China Agricultural University filed Critical China Agricultural University
Priority to CNB2004100428788A priority Critical patent/CN1331609C/en
Publication of CN1704168A publication Critical patent/CN1704168A/en
Application granted granted Critical
Publication of CN1331609C publication Critical patent/CN1331609C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Devices For Use In Laboratory Experiments (AREA)

Abstract

The present invention relates to a measuring device for extracting quantitative liquid, which is characterized in that the present invention comprises a measuring device for extracting quantitative liquid, a driving device, a sleeve barrel, a connecting rod, a needle head piston, a liquid sucking piston, a spring and a conical pipe type needle head, wherein an extending piston rod is arranged in the driving device, the top part of the sleeve barrel is connected with the driving device, and the bottom of the sleeve barrel is provided with an inner edge; the top part of the connecting rod is connected with the piston rod of the driving device and inserted in the sleeve barrel; the top outer edge of the needle head piston is glidingly arranged in the sleeve barrel, the diameter of the lower outer edge of the needle head piston is slightly less than the inner edge of the sleeve barrel; a through hole is arranged in the bottom of the needle head piston; one end of the liquid sucking piston is connected with the connecting rod, and the other end of the liquid sucking piston is glidingly arranged in the needle head piston; the spring is arranged at the upper part of the liquid sucking piston, one end of the spring is fixed to the connecting rod, and the other end of the spring is fixed to the needle head piston; the mounting end of the conical pipe type needle head is provided with a concave hole which can be sheathed at the bottom of the needle head piston. The present invention can be used for extracting quantitative liquid without cross contamination among liquid to be measured, the accuracy of an experiment is ensured, the program of cleaning equipment is eliminated, experimental efficiency is increased, and the measuring device for extracting quantitative liquid can be used for extracting micro liquid as well as widely used in the process of displacing various reagent or microbe culture solutions.

Description

Quantitative liquid taking device
Technical field
The present invention relates to a kind of liquid and pipette device, particularly about the quantitative liquid taking device of a kind of reagent or microbial culture medium.
Background technology
In biological, chemical experiment, usually need to the nutrient solution of biological, microorganism and reagent pipette or quantitatively pipette operation, carry out these operations, use glass apparatus such as glue head dropper, pipette usually.Even but these instruments itself are made again for accurate, also all can not well solve two very important problem.
It at first is accuracy problem, at present in the manual operations amount of reagent of getting how much, except that part is determined by instrument precision, all the other all are experience and the judgements of depending on the experimenter, even operate very skilled experimenter, can not guarantee that the dosage of each reagent of getting under the same terms is in full accord; Because the experimental work amount is bigger, experimenter's fatigue often makes and occurs some errors in the manual operations, requires strict experiment for some sometimes, the loss that error causes occurs and usually can't retrieve.
Next is that the existing liquid instrument of getting mostly is contact and gets liquid, when getting different test solutions, preceding a kind of test solution of getting in the liquid instrument is cleaned, to avoid a kind of test solution in back contaminated; Yet the clean-up performance of cleaning instrument required time and cleaning etc. all can bring influence in various degree to experiment process and experiment effect.
Summary of the invention
At the problems referred to above, the purpose of this invention is to provide a kind of employing noncontact mode and get liquid, and get liquid measure stability height, and can improve the quantitative liquid taking device of conventional efficient.
For achieving the above object, the present invention takes following technical scheme: a kind of quantitative liquid taking device is characterized in that it comprises: a drive unit is provided with a piston rod that stretches out in it; One sleeve, its top is connected with described drive unit, and its bottom has an inner edge; One connecting rod, its top is connected with the piston rod of described drive unit, and is plugged in the described sleeve; One syringe needle piston, its top outer rim is slidingly arranged in the described sleeve, and the bottom external profile diameter is slightly less than described sleeve inner edge, and described syringe needle piston base has a through hole; One imbibition piston, the one end connects described connecting rod, and the other end is slidingly arranged in the described syringe needle piston; One spring, it is set in described imbibition piston top, and described spring one end is fixed on the described connecting rod, and the other end is fixed on the described syringe needle piston; One conical tube type syringe needle, its installation end have a shrinkage pool that can be set in described syringe needle piston base.
Described syringe needle is a disposable aspiration needle.
The present invention can also comprise a needle holder, and it has a centre bore, and has the concave station of the described syringe needle of a support at described needle holder top.
The present invention is owing to take above technical scheme, and it has the following advantages: 1, the present invention can be connected with piston rod with the cylinder body of drive unit respectively when therefore using owing to be provided with sleeve and connecting rod, realizes getting the work that surges.2, of the present invention owing on connecting rod, connected an imbibition piston, and connect a syringe needle piston that is arranged on imbibition piston outside by spring, can drive the syringe needle piston by spring force when therefore getting liquid and get syringe needle, get liquid by the imbibition piston, after getting liquid and finishing, can retract the syringe needle piston by spring force, and when the syringe needle piston is return, utilize of the structure setting of the installation portion of syringe needle, unload automatically and finished the disposable aspiration needle of getting the liquid conduct greater than the sleeve inner edge.3, the present invention is owing to be to adopt structure control to get liquid measure fully, rather than relies on artificial experience to get liquid, therefore, can realize the quantitative liquid getting of strict conformance, and then guarantee the true and reliable property of experiment structure.4, the present invention is because to get liquid measure very little at every turn, and liquid is contaminated pinhead itself only, after using up syringe needle is backed off at every turn, concentrates cleaning and sterilizing, and needn't other parts be cleaned or sterilize, so the raising conventional efficient of can saving time.5, the present invention is because owing to can adopt disposable liquid tubular type syringe needle, and this syringe needle generally can be made with plastic material with low cost, need not clean after being used to complete, only need to concentrate to abandon or handle, further save the operation of cleaning and sterilizing syringe needle.The present invention can be widely used in all ingredients or microbial culture medium pipettes the process operation, also can be applied in the occasion that other needs trace to get liquid.
Description of drawings
Fig. 1 is a structural representation of the present invention
The specific embodiment
As shown in Figure 1, the present invention includes a sleeve 1, its top has an outer rim 11, and its bottom has an inner edge 12, has some installing holes 13 on the outer rim 11.One connecting rod 2, it is arranged in the sleeve 1, and connecting rod 2 tops have a screw 21, and it can be connected on the piston rod 9 of drive unit 8 (such as cylinder, hydraulic cylinder etc.) in use.One syringe needle piston 3, its top outer rim 31 is slidingly arranged in the sleeve 1, and outer rim 32 diameters in its underpart are slightly less than the inner edge 12 of sleeve 1, so that can pass from inner edge 12, have a through hole 33 in the bottom of syringe needle piston 3.One imbibition piston 4, one end are stocks 41, and the top of stock 41 is fixed on the bottom of connecting rod 2, and the other end of imbibition piston 4 is slidingly arranged in the syringe needle piston 3.One spring 5, it is set on the stock 41 of imbibition piston 4, and an end of spring 5 is fixed on the connecting rod, and the other end is fixed on the syringe needle piston 3.Several disposable conical tube type syringe needles 6, the installation end of each syringe needle 6 has a shrinkage pool 61 that can be set in syringe needle piston 3 bottoms, because the effect of syringe needle 6 only is imbibition, therefore can adopt more expensive materials such as metal, and adopt the dog-cheap disposable plastic syringe needle of price.
For with the supporting use of integral device of another invention of the applicant " a kind of liquid spray equipment of getting " (separate case is applied for a patent), the present invention can also be provided with a needle holder 7, have a centre bore 71 on the needle holder 7, and have the concave station 72 of a support 6 syringe needles at needle holder 7 tops.
The present invention is that the power set with integral device are used, at first there is the needle holder 7 of syringe needle 6 to be pushed into the bottom of sleeve 1 support by artificial or conveying device, and startup drive unit 8, making its piston rod 9 drive connecting rod 2 of the present invention moves downward, the imbibition piston 4 of connecting rod 2 ends just moves downward in syringe needle piston 3, meanwhile, the spring 5 of pressurized will promote syringe needle piston 3 and stretch out sleeve 1 downwards, after the bottom of syringe needle piston 3 embeds the concave station 72 of disposable aspiration needle 6, piston rod 9 stop motions, syringe needle 6 just is enclosed within syringe needle piston 3 ends, finishes and gets the syringe needle action.
The whole then power set 8 of getting the liquid spray equipment move drive unit 8 of the present invention on the whole, and move to the place that holds reagent or microbial culture medium, piston rod continues to drive syringe needle piston 3 by connecting rod 2 and spring 5 and moves downward, prop up the bottom inner edge 12 of sleeve 1 until the top of syringe needle piston 3 outer rim 31, syringe needle piston 3 no longer moves relative to sleeve 1, and piston rod 9 continues to move down by connecting rod 2 drive imbibition pistons 4, extrude the gas in the syringe needle piston 3, be against the through hole 33 of syringe needle piston 3 bottoms until imbibition piston 4 bottoms, the exhaust action is finished in piston rod 9 stop motions.
Then the piston rod 9 of drive unit 8 drives connecting rods 2 and oppositely upwards regains, because spring 5 was in and returned to form this moment, so spring 5 still keeps the downward pressure of syringe needle piston 3, and syringe needle piston 3 keeps motionless, imbibition piston 4 imbibition that moves upward.Because liquid absorption is very little, so liquid exists only in the syringe needle 6, and can upwards not enter other position.
When the imbibition action is finished, imbibition piston 4 has withstood on the top of syringe needle piston 3, and piston rod 9 also drives spring 5 by connecting rod 2 and syringe needle piston 3 moves upward, pass sleeve 1 bottom inner edge 12 until syringe needle piston 3 bottom outer rims 32, and syringe needle 6 top card are in the bottom of inner edge 12, can not continue to move upward, and be pushed out the bottom of syringe needle piston 3, the withdraw of the needle 6 action are finished.
Action description when the embodiment of said apparatus and operation; be in order to be illustrated more clearly in technical scheme of the present invention; but should be as the restriction of protection domain of the present invention; the concrete structures more of the present invention and the kind of drive all can change to some extent; such as all can changing specific to the shape of sleeve 1, syringe needle piston 3, imbibition piston 4 and syringe needle 5 and the material of use, the connected mode of each parts and with the setting of the tie point of other single unit system be connected employed securing member and also can change.

Claims (3)

1, a kind of quantitative liquid taking device is characterized in that, it comprises:
One drive unit is provided with a piston rod that stretches out in it;
One sleeve, its top is connected with described drive unit, and its bottom has an inner edge;
One connecting rod, its top is connected with the piston rod of described drive unit, and is plugged in the described sleeve;
One syringe needle piston, its top outer rim is slidingly arranged in the described sleeve, and the bottom external profile diameter is slightly less than described sleeve inner edge, and described syringe needle piston base has a through hole;
One imbibition piston, the one end connects described connecting rod, and the other end is slidingly arranged in the described syringe needle piston;
One spring, it is set in described imbibition piston top, and described spring one end is fixed on the described connecting rod, and the other end is fixed on the described syringe needle piston;
One conical tube type syringe needle, its installation end have a shrinkage pool that can be set in described syringe needle piston base.
2, quantitative liquid taking device as claimed in claim 1 is characterized in that: described syringe needle is a disposable aspiration needle.
3, quantitative liquid taking device as claimed in claim 1 or 2 is characterized in that: it also comprises a needle holder, and described needle holder has a centre bore, and has the concave station of the described syringe needle of a support at described needle holder top.
CNB2004100428788A 2004-05-28 2004-05-28 Quantitative liquid extraction device Expired - Fee Related CN1331609C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CNB2004100428788A CN1331609C (en) 2004-05-28 2004-05-28 Quantitative liquid extraction device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CNB2004100428788A CN1331609C (en) 2004-05-28 2004-05-28 Quantitative liquid extraction device

Publications (2)

Publication Number Publication Date
CN1704168A CN1704168A (en) 2005-12-07
CN1331609C true CN1331609C (en) 2007-08-15

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Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105486540B (en) * 2016-01-15 2018-04-24 杭州景业智能科技有限公司 The accurate sampler of dusty material
CN108395982B (en) * 2018-05-30 2024-04-23 厦门市科环海洋生物科技有限公司 Colony spraying device
CN110057624B (en) * 2019-04-28 2024-04-05 深圳市大肯科技有限公司 Air pump
CN115920992B (en) * 2022-12-13 2023-09-26 康容生物科技(太仓)有限公司 Anti-vibration automatic pipetting device and use method thereof

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2068418U (en) * 1989-12-21 1991-01-02 西南石油学院 Batch liquid-removing apparatus for chemical test
US5355738A (en) * 1991-01-07 1994-10-18 Labsystems Oy Positive-displacement pipette
CN1248495A (en) * 1998-09-22 2000-03-29 陈国君 Quick adjustable pipette
CN2447765Y (en) * 2000-09-05 2001-09-12 王振明 Pipet
US20030099576A1 (en) * 2001-09-10 2003-05-29 Xingguo Li Fibrous self-sealing components
US20030099578A1 (en) * 2001-10-16 2003-05-29 Richard Cote Hand-held pipettor

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2068418U (en) * 1989-12-21 1991-01-02 西南石油学院 Batch liquid-removing apparatus for chemical test
US5355738A (en) * 1991-01-07 1994-10-18 Labsystems Oy Positive-displacement pipette
CN1248495A (en) * 1998-09-22 2000-03-29 陈国君 Quick adjustable pipette
CN2447765Y (en) * 2000-09-05 2001-09-12 王振明 Pipet
US20030099576A1 (en) * 2001-09-10 2003-05-29 Xingguo Li Fibrous self-sealing components
US20030099578A1 (en) * 2001-10-16 2003-05-29 Richard Cote Hand-held pipettor

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Publication number Publication date
CN1704168A (en) 2005-12-07

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Granted publication date: 20070815

Termination date: 20120528