CN1327051A - Method for producing cellulase - Google Patents
Method for producing cellulase Download PDFInfo
- Publication number
- CN1327051A CN1327051A CN 00107965 CN00107965A CN1327051A CN 1327051 A CN1327051 A CN 1327051A CN 00107965 CN00107965 CN 00107965 CN 00107965 A CN00107965 A CN 00107965A CN 1327051 A CN1327051 A CN 1327051A
- Authority
- CN
- China
- Prior art keywords
- bacterial classification
- culture
- production
- cellulose enzyme
- temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The cellulase producign process includes the steps of strain selection, culture inside test tube and Erlenmeyer flask, culture inside seed jar while stirring, fermenting culture inside fermentation tank by adding xylose dregs, nutritious salt, trace elements and strain from seed jar culture into the tank, press filtering of the fermented liquid, superfiltering concentration of the press filtered material, adding glycerine protecting agent in 5 % to prepare liquid cellulase preparation, and spray drying to prepare powder. The said process is simple, low in cost and pollution-less, and is suitable for large scale industrial production.
Description
The present invention relates to a kind of method of production of cellulose enzyme, particularly a kind of method with xylose residue production of cellulose enzyme.
At present, both at home and abroad the production method of cellulase generally adopts liquid submerged fermentation, and the carbon source that is used to produce enzyme generally is pure cellulose or paper pulp, complex manufacturing, and cost is higher, and environmental pollution is serious.
Purpose of the present invention just is to overcome weak point of the prior art, provides a kind of technology simple, and cost is low, and is pollution-free, utilizes xylose residue to add the method that strain fermentation is cultivated the production of cellulose enzyme.
The object of the present invention is achieved like this, and it may further comprise the steps:
A. choose bacterial classification, under 30-32 ℃ temperature condition, putting into test tube cultivated 7-10 days, putting into triangular flask then cultivated under 30-32 ℃ temperature condition 7-10 days again, at last bacterial classification is put into the seeding tank stir culture, the stir culture time of bacterial classification in seeding tank is 24 hours, and temperature is 36 ± 2 ℃;
B. the bacterial classification of cultivating in 2% xylose residue, 0.5 ‰ nutritive salt, 0.05 ‰ trace element, the seeding tank is put into 5M
3Stir fermentation culture in the fermentor tank, fermented incubation time 48 hours, 36 ± 2 ℃ of temperature, pH value=6.0, stirring velocity is 120 rev/mins;
The fermented liquid that c. will stir after fermenting carries out press filtration, and filter cloth order number is 664, and speed of filter pressing is 4 tons/hour;
D. with the further ultrafiltration and concentration of the material after the press filtration, make enzyme liquid concentration concentrate 10-15 doubly;
E. the glycerine protective material of interpolation 5% is made the liquid cellulase preparation behind the ultrafiltration and concentration, makes pulvis after the spraying drying;
Order of the present invention also can be realized by following technical measures, selected bacterial classification is the Trichodermarcesei Rut C30 of Zhejiang University's Laboratory Production, the spraying drying temperature is 180 ℃, time is 0.1-8.5 second, nutritive salt is sulfate of ammoniac or sal epsom, phosphoric acid dioxy potassium, and trace element is ferrous sulfate, cobaltous chloride.
Compared to existing technology, the present invention has the following advantages:
1. production technique is simple, and operation is implemented convenient;
2. raw material sources are extensive, can utilize xylose residue, corn stalk, corncob cellulose to do raw material;
3. production cost is low, and is pollution-free;
4. product performance are stable, and enzyme activity (FPA) can reach 5-12IU/ml;
Below in conjunction with embodiment in detail the present invention is described in detail, at first choose bacterial classification, under 30-32 ℃ temperature condition, putting into test tube cultivated 7-10 days, putting into triangular flask then cultivated under 30-32 ℃ temperature condition 7-10 days again, at last bacterial classification is put into the seeding tank stir culture, the stir culture time of bacterial classification in seeding tank is 24 hours, and temperature is 36 ± 2 ℃.The bacterial classification of cultivating in 2% xylose residue, 0.5 ‰ nutritive salt, 0.05 ‰ trace element, the seeding tank is put into 5M
3Stir fermentation culture in the fermentor tank, fermented incubation time 48 hours, 36 ± 2 ℃ of temperature, pH value=6.0, stirring velocity is 120 rev/mins.The fermented liquid that stirs after fermenting is carried out press filtration, and filter cloth order number is 664, and speed of filter pressing is 4 tons/hour.With the further ultrafiltration and concentration of the material after the press filtration, make enzyme liquid concentration concentrate 10-15 doubly.The glycerine protective material of interpolation 5% is made the liquid cellulase preparation behind the ultrafiltration and concentration, makes pulvis after the spraying drying.Selected bacterial classification is the Trichodermarcesei Rut C30 of Zhejiang University's Laboratory Production, the enzyme activity height, stable performance, anti-assorted bacterium ability is strong, the spraying drying temperature of powder process agent is 180 ℃, time is 0.1-8.5 second, and nutritive salt is sulfate of ammoniac or sal epsom, phosphoric acid dioxy potassium, and trace element is ferrous sulfate, cobaltous chloride.Production technique of the present invention is simple, and operation is implemented convenient, and raw material sources are extensive, can utilize xylose residue, corn stalk, corncob cellulose to do raw material, and production cost is low, and is pollution-free; Product performance are stable, and enzyme activity (FPA) can reach 5-12IU/ml; And having saved common raw materials pretreatment operation, be suitable for large-scale industrial production, is a kind of production method of ideal cellulase.
Claims (5)
1. the method for a production of cellulose enzyme may further comprise the steps:
A. choose bacterial classification, under 30-32 ℃ temperature condition, putting into test tube cultivated 7-10 days, putting into triangular flask then cultivated under 30-32 ℃ temperature condition 7-10 days again, at last bacterial classification is put into the seeding tank stir culture, the stir culture time of bacterial classification in seeding tank is 24 hours, and temperature is 36 ± 2 ℃;
B. the bacterial classification of cultivating in 2% xylose residue, 0.5% nutritive salt, 0.05% trace element, the seeding tank is put into 5M
3Stir fermentation culture in the fermentor tank, fermented incubation time 48 hours, 36 ± 2 ℃ of temperature, pH value=6.0, stirring velocity is 120 rev/mins;
The fermented liquid that c. will stir after fermenting carries out press filtration, and filter cloth order number is 664, and speed of filter pressing is 4 tons/hour;
D. with the further ultrafiltration and concentration of the material after the press filtration, make enzyme liquid concentration concentrate 10-15 doubly;
E. the glycerine protective material of interpolation 5% is made the liquid cellulase preparation behind the ultrafiltration and concentration, makes pulvis after the spraying drying;
2. according to the method for the described production of cellulose enzyme of claim 1, it is characterized in that: selected bacterial classification is the Trichodermarcesei Rut C30 of Zhejiang University's Laboratory Production.
3. according to the method for the described production of cellulose enzyme of claim 1, it is characterized in that: the spraying drying temperature is 180 ℃, and the time is 0.1-8.5 second.
4. according to the method for the described production of cellulose enzyme of claim 1, it is characterized in that: nutritive salt is sulfate of ammoniac or sal epsom, phosphoric acid dioxy potassium.
5. according to the method for the described production of cellulose enzyme of claim 1, it is characterized in that: trace element is ferrous sulfate, cobaltous chloride.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00107965 CN1327051A (en) | 2000-06-02 | 2000-06-02 | Method for producing cellulase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00107965 CN1327051A (en) | 2000-06-02 | 2000-06-02 | Method for producing cellulase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1327051A true CN1327051A (en) | 2001-12-19 |
Family
ID=4579059
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00107965 Pending CN1327051A (en) | 2000-06-02 | 2000-06-02 | Method for producing cellulase |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1327051A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1298846C (en) * | 2005-01-18 | 2007-02-07 | 清华大学 | Process for producing cellulase by semicontinuous solid fermentation-extraction coupling |
| CN1298847C (en) * | 2005-01-18 | 2007-02-07 | 清华大学 | Process for electric field enhanced solid fermentation preparation of cellulase |
| CN1807610B (en) * | 2006-01-23 | 2011-01-26 | 迟乃玉 | Method for producing low temperature cellulase using microbe fermentation |
| CN102766615A (en) * | 2012-07-20 | 2012-11-07 | 唐山市冀东溶剂有限公司 | Method for preparing cellulase by bacilli |
| CN102876648A (en) * | 2012-10-25 | 2013-01-16 | 天津工业生物技术研究所 | Method for extracting cellulase from microorganism fermentation broth |
| CN102876647A (en) * | 2012-10-16 | 2013-01-16 | 熊鹏 | Cellulase separation and purification method |
-
2000
- 2000-06-02 CN CN 00107965 patent/CN1327051A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1298846C (en) * | 2005-01-18 | 2007-02-07 | 清华大学 | Process for producing cellulase by semicontinuous solid fermentation-extraction coupling |
| CN1298847C (en) * | 2005-01-18 | 2007-02-07 | 清华大学 | Process for electric field enhanced solid fermentation preparation of cellulase |
| CN1807610B (en) * | 2006-01-23 | 2011-01-26 | 迟乃玉 | Method for producing low temperature cellulase using microbe fermentation |
| CN102766615A (en) * | 2012-07-20 | 2012-11-07 | 唐山市冀东溶剂有限公司 | Method for preparing cellulase by bacilli |
| CN102876647A (en) * | 2012-10-16 | 2013-01-16 | 熊鹏 | Cellulase separation and purification method |
| CN102876647B (en) * | 2012-10-16 | 2014-02-12 | 熊鹏 | Cellulase separation and purification method |
| CN102876648A (en) * | 2012-10-25 | 2013-01-16 | 天津工业生物技术研究所 | Method for extracting cellulase from microorganism fermentation broth |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101735993B (en) | Method for efficiently producing cellulase | |
| CN104311337B (en) | A kind of method of utilizing glutamic acid wastewater to produce liquid fertilizer | |
| CN110241041B (en) | Compound microbial preparation, preparation method and application thereof | |
| CN102363795A (en) | Method for co-production of lactic acid and alcohol by lignocellulose | |
| CN104341536A (en) | Method for high-efficiency extraction of nutrient substances in seaweed | |
| CN104230444B (en) | A kind of method utilizing sodium glutamate production waste material to prepare fertilizer | |
| CN100595271C (en) | Method of preparing culture medium carbon source for producing bacteria cellulose | |
| CN101781667B (en) | Method for producing bacterial cellulose with wheat straws/maize straws | |
| CN104341535A (en) | Enteromorpha prolifera high-valued extraction method | |
| CN110759754B (en) | Harmless treatment and resource utilization method of glucosamine fermentation bacterium residues | |
| CN1327051A (en) | Method for producing cellulase | |
| CN100529055C (en) | Bacillus alcaligenes and its application in preparing Weilan gum | |
| CN102173879B (en) | Method for producing biological potassium fertilizer by utilizing cellulose fermented waste mycelium and biogas residue | |
| CN111893149A (en) | Method for preparing bacterial cellulose by utilizing soapberry fruit shells | |
| CN1114696C (en) | Process for producing alcohol with xylose slag | |
| CN101875926B (en) | Method for preparing liquid cellulase | |
| CN109970495A (en) | A kind of technique preparing organic fertilizer using amino acid fermentation tail washings | |
| CN110257305B (en) | Cultivation method of Shewanella with high yield of alkaline protease | |
| CN104341233A (en) | Method for high-efficiency extraction of nutrient substances in undaria pinnatifida sporophyll | |
| CN1114695C (en) | Method for producing alcohol by using plant fiber as raw material | |
| CN112266300A (en) | Method for fermenting algae microorganism using bacillus | |
| CN102071179A (en) | Method for producing cellulase through submerged fermentation of aspergillus niger liquid | |
| CN101781668A (en) | Method for producing bacterial cellulose with wheat straws/spruces | |
| CN104938762A (en) | Preparation method of duckweed protein powder for fermentation | |
| CN112522108B (en) | Water-soluble micromolecular organic carbon algae nutrient and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |