CN1322951A - Red sage medicine fingerprint establishing method and standard fingerprint atlas - Google Patents
Red sage medicine fingerprint establishing method and standard fingerprint atlas Download PDFInfo
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- CN1322951A CN1322951A CN 01119925 CN01119925A CN1322951A CN 1322951 A CN1322951 A CN 1322951A CN 01119925 CN01119925 CN 01119925 CN 01119925 A CN01119925 A CN 01119925A CN 1322951 A CN1322951 A CN 1322951A
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Abstract
The present invention relates to the establishment of the fingerprint of water soluble red sage extract HPLC and standard fingerprint. Testee solution is first prepared with tested red sage medicine powder and weighing, mixing with water, reflux extraction, methanol dissolution, centrifugation and taking supernatant; contrast solution is then prepared with danshensu sodium and protocatechuric aldehyde; and the fingerprint is finally established through determination of the contrast solution and testee solution through liquid phase chromatographic process. The fingerprint of testee red sage sample is compared with standard fingerprint to test the quality and truthness of red sgae medicine.
Description
The present invention relates to the method for building up of the finger-print of Chinese medicine, specifically the method for building up of water-soluble extract of red sage root HPLC finger-print the invention still further relates to the resulting red rooted salvia standard finger-print of method thus.
The red sage root is the dry root and rhizome of labiate red sage root Salvia miltiorrhiza Bge..All produce in most of area, the whole nation.Because the red sage root causes quality uneven because of kind, the place of production, picking time are different, thereby its manufactured goods quality of stability also is difficult to guarantee.At present, to the evaluation of the red sage root and compound preparation thereof, choose one, two active component or the index components of the red sage root often and carry out assay, and how much judge quality with its content.For example, with tanshinone IIA content (Chinese Pharmacopoeia 2000 version one one 58 pages) or content of Danshensu (Zhang Youqin etc., traditional Chinese medicine journal, 2000,28 (3): 68) wait the quality of differentiating red rooted salvia; Judge red sage root kind and place of production situation (Qiu Feijun, contemporary Chinese application pharmacy, 1998,15 (5): 16 with tanshinone; Hu Shilin etc., CHINA JOURNAL OF CHINESE MATERIA MEDICA, 1999,24 (12): 721); With content of Danshensu (Yan Changkai etc., Chinese Hospitals pharmaceutical journal, 2000,600), protocatechualdehyde content (Zheng end crystalline substance etc., Chinese Pharmaceutical Affairs, 2,000 20 (10):, 254) or tanshinone IIA content (Lin Weizhong etc. 14 (4):, Chinese patent drug, 766) etc. 2000,22 (11): the quality of differentiating compound red sage root preparation, differentiate the quality (Shao Shuijuan of the Fufang Danshen Pian that different manufacturers is produced with tanshinone IIA content, China's medicine company, 2000,9 (7): 27) etc.The chemical constitution of the known red sage root has tens kinds, and its liposoluble constituent mostly is quinoid reddish yellow material greatly, as salvia miltiorrhiza bge I, II A, II B, and red sage root quinone A, B, C, red sage root acid potassium fat, Isotanshinone, II, Cryptotanshinone etc.Its water soluble ingredient mostly is phenol aldehyde, phenol acid, diterpenoid acid greatly, as succinic acid, salviandic acid A, B, C, and 3,4-dihydroxy-benzoic acid etc.In addition, also isolate cupreol, vitamin E etc. (Wang Baixiang chief editor, traditional Chinese medical science liver and bladder disease is learned, first published, Chinese Medicine science and technology publishing house,, 96 pages in 1993).If with one, the active component of two kind of red sage root illustrates the inherent quality of the red sage root and prescribed preparation thereof to have certain one-sidedness, said nothing of the index components of no drug effect.Control the effect of the red sage root and prescribed preparation thereof, just can not must be controlled its material group integral body only at one, two chemical constitutions.So, except " micro-analysis ", also use certain " macroanalysis " method, characterize traditional Chinese medicine quality on the whole effectively.That traditional Chinese medicine fingerprint is meant is common in certain Chinese crude drug or the Chinese patent drug, have distinctive certain class or the chromatogram of number constituents or the collection of illustrative plates of spectrum.Do not have under the clear and definite situation in the present stage Effective Components of Chinese Herb overwhelming majority, the quality for effective control Chinese crude drug or Chinese patent drug has great importance.The Japan main manufacturing enterprise of Chinese prescription medicine just adopts the high-efficiency liquid-phase fingerprint control of quality in enterprises in the eighties in 20th century.Germany, France find that the medical function of ginkgo biloba p.e is extract gained material group's mass action result in the process that ginkgo biloba p.e is developed jointly, and to the quality control of such integral body, also adopt the high-efficiency liquid-phase fingerprint method.In the plant herbal medicine guide of formulating U.S. FDA recent years clearly the method for quality control (FDA.Guidance of Industry:Botanical Drug (Draft) .2000 August) of finger-print as the compounding substances group.Finger-print is become a consensus of the international community at present as Chinese herbal medicine and extraction of substance amount control method thereof.Now, more to the assay method of active component such as tanshinone, danshensu etc. in the red sage root, but the method for building up of red sage root finger-print is not appeared in the newspapers.Be to solve the problem of red sage root quality shakiness, the applicant carried out investigating relatively to the different places of production red sage root, built up a day red sage root base, scholar's power Shanglou in Shanglou in 1997, and the GAP management is implemented in the base.For setting up the integrated control method of red rooted salvia quality, special with State Drug Administration 2000 issue about the requirement of Chinese crude drug fingerprint pattern technology, 10 batches of medicinal materials of the red sage root to red sage root base, scholar's power Shanglou, sky are measured, and set up red sage root finger print measuring method.
The objective of the invention is by research water-soluble extract of red sage root HPLC finger-print, find out a kind of method of red sage root quality control, the method of the standard finger-print of the red sage root is set up in establishment, whereby can be with red sage root dactylogram as one of index of quality control and real and fake discrimination.The present invention also provides the standard finger-print of the red sage root simultaneously.
The present invention implements through the following steps.
The method that a kind of red rooted salvia finger-print is set up, it is to take following steps:
The preparation of step 1 need testing solution: the red rooted salvia powder of getting 30 mesh sieves adds water, and precision is weighed; Refluxing extraction 1-3 hour, put and be chilled to room temperature, supply dehydration; Get extract and put on a small quantity in the measuring bottle, methanol constant volume is to scale, and is centrifugal, gets supernatant as need testing solution;
The preparation of step 2 reference substance solution: it is an amount of to get reference substance, with the moving phase dilution, makes reference substance solution;
The preferred embodiments of the invention are as follows:
(1) chromatographic condition and system suitability test: chromatographic column is that octadecylsilane chemically bonded silica is a filler; Moving phase is methanol-water-glacial acetic acid (8: 92: 1); Detect wavelength 254 ± 1nm; Theoretical cam curve is calculated with the danshensu peak, should be not less than 2500;
(2) need testing solution preparation: get red rooted salvia powder (crossing 30 mesh sieves) 4.00g, add water 50ml, precision is weighed, refluxing extraction 2 hours, put and be chilled to room temperature, supply dehydration, get extract 2ml and put the 25ml measuring bottle, methanol constant volume is to scale, and is centrifugal, gets supernatant as need testing solution;
(3) preparation of reference substance solution: get Sodium Danshensu, the protocatechualdehyde reference substance is an amount of, dilutes with moving phase, makes to contain Sodium Danshensu 20 μ g among every 1ml, protocatechualdehyde 5.0 μ g, product solution in contrast;
(4) determination method: accurate respectively reference substance solution and need testing solution each 20 μ l, 10 μ l of drawing, inject liquid chromatograph respectively, according to high effective liquid chromatography for measuring, write down 80 minutes chromatogram, promptly.With the relative retention time of the chromatographic peak (S peak) of protocatechualdehyde and peak area is 1 to calculate relative retention time and relative peak area;
(5) set up red sage root standard finger-print, its similarities and differences are distinguished in red sage root sample finger-print to be measured and standard finger-print contrast.
Principle of the present invention is to be its main active according to red sage root water soluble ingredient, sets up water-soluble extract of red sage root HPLC finger-print, holds the kind and the quality situation of the red sage root from the whole facial feature of chromatogram.
Advantage of the present invention is as follows:
(1) the HPLC finger-print of setting up with water-soluble extract of red sage root is being represented the most of pharmacologically active of the red sage root, can characterize the quality of red rooted salvia effectively.Whole chemical constitutions to the red sage root detect, and both can not also there is no need.A large amount of pharmacology and clinical research result show that the activity of the water soluble ingredient of the red sage root is better than liposoluble constituent, so just have more representativeness with the water soluble ingredient of the red sage root as monitor control index.
(2) do as a wholely to treat with the whole fingerprint graph of the red sage root, pay attention to each front and back that constitute fingerprint characteristic peak order and mutual relationship, pay attention to whole facial feature, both avoided judging the one-sidedness of red sage root total quality that having reduced again was the possibility of the artificial processing of requisite quality because of only measuring one, two chemical constitution.The present invention will contribute for quality and the curative effect that improves the red sage root and prescribed preparation thereof for quality complete, that accurately estimate the red sage root provides new reference standard.
(3) the present invention have that method is easy, stable, precision is high, favorable reproducibility, the characteristics that are easy to grasp.The detection wavelength that is used to measure danshensu or protocatechualdehyde in the document is generally 281 ± 1nm (Zhang Youqin etc., traditional Chinese medicine journal, 2000,28 (3): 68; Zheng end crystalline substance etc., Chinese Pharmaceutical Affairs, 2000,14 (4): 254), but finger-print is not in order to measure the accurate content of certain composition, but will fully reflect the information of chemical constitution.Compare with detecting wavelength 281 ± 1nm, measure with 254nm, it is more to go out the peak, and the information of reaction is more complete, so adopt 254nm to be the detection wavelength, this also is the difference with existing method.In addition, for measuring the content of certain chemical constitution in the red sage root, literature method often will carry out complicated processing to sample, as (Li Wenchun etc., Heilungkiang medicine, 1999 such as water extract-alcohol precipitation, mistake polymeric adsorbents, 12 (24): 193), and the present invention only needs to carry with hydro-thermal, and sample preparation is greatly easy, has saved the cost and the time of check.
Embodiments of the invention are as follows.1 instrument and reagent 1.1 instruments
Hewlett-Packard's 1100 high performance liquid chromatographs; The VWD of Hewlett-Packard ultraviolet variable-wavelenght detector; The star workstation of the U.S.'s safe chromatogram difficult to understand.1.2 reagent
The Sodium Danshensu reference substance, self-control, purity is greater than 99%; The protocatechualdehyde reference substance, commercially available analysis is pure refining, and purity is greater than 99%; Methyl alcohol, chromatographically pure; Glacial acetic acid is analyzed pure; Distilled water; Red rooted salvia is produced the red sage root by a day red rooted salvia base, scholar power Shanglou.The commercially available red sage root is available from city's medicinal material company.2 methods and result's 2.1 chromatographic conditions
Chromatographic column: octadecyl silane (4.6 * 250mm, 5 μ) U.S. Alltech company; Moving phase: methanol-water-glacial acetic acid (8: 92: 1); Detect wavelength: 254nm; Flow velocity: 1ml/min; Column temperature: 40 ℃; Theoretical cam curve is calculated with the danshensu peak, should be not less than 2500.2.2 detect selection 3 determining fingerprint patterns 3.1 red sage root finger-prints preparation of wavelength
Sodium Danshensu is got in the preparation of reference substance solution, and the protocatechualdehyde reference substance is an amount of, dilutes with moving phase, makes to contain Sodium Danshensu 20 μ g among every 1ml, protocatechualdehyde 5.0 μ g, product solution in contrast.
It is object of reference (S) that object of reference is selected protocatechualdehyde for use.
Red rooted salvia powder (crossing 30 mesh sieves) 4.00g is got in the need testing solution preparation, adds water 50ml, and precision is weighed, and refluxing extraction 2 hours is put and is chilled to room temperature, supplies dehydration, gets 2ml and puts the 25ml measuring bottle, and methanol constant volume is to scale, and the centrifuging and taking supernatant is as need testing solution.
Get reference substance and need testing solution 20 μ l, 10 μ l sample introductions respectively,, write down 80 minutes chromatogram according to high effective liquid chromatography for measuring.With the relative retention time of the chromatographic peak (S peak) of protocatechualdehyde and peak area is 1 to calculate relative retention time and relative peak area.3.2 total peak is determined
By 10 batches of Shanglou red rooted salvia determining fingerprint patterns, compare its chromatogram, determine that total peak is 12, wherein be with the protocatechualdehyde object of reference to surpass total peak area 10% fingerprint peaks be No. 1 peak (RT0.183), No. 8 peaks (danshensu, RT0.525), No. 11 peaks (protocatechualdehyde, RT1.00) and No. 12 peaks (RT2.576), see Fig. 2-Shanglou red rooted salvia.Total peak relative retention time of the 10 batches of medicinal materials and relative peak area statistics see Table 1, table 2.3.3 finger-print reappearance test
With 0801 batch of Shanglou red rooted salvia is test sample (N=5), according to the operation of finger-print reappearance prescriptive procedure, respectively the relative retention time at total peak and the peak area that relative peak area has regulation is added up, and RSD% is no more than 3%.The results are shown in Table 3.
10 batches of red rooted salvia peak area statistics of table 1
| Total fingerprint peaks medicinal material lot number | ???101 | ???301 | ???502 | ???701 | ????801 | ??0902 | ??1001 | ??1101 | ???1102 | ??1103 | Mean value SD |
| 11-S peak area peak area peak, 8 relative peak area % peak, 1 relative peak area % peak area peak, | ??37014 ??40.78 ??37958 ??41.82 ??90776 ??40032 ??44.1 ??0.73 | ??42680 ??52.17 ??45282 ??55.35 ??81812 ??28324 ??34.62 ??0.84 | ??48958 ??47.91 ??47472 ??46.46 ??1E+05 ??30000 ??29.36 ??0.96 | ??41187 ??53.32 ??40352 ??52.23 ??77251 ??29688 ??38.43 ??0.81 | ???45823 ???48.54 ???40495 ???42.9 ???94399 ???37166 ???39.37 ???0.9 | ??42694 ??40.97 ??40646 ??39.01 ??104201 ??30048 ??28.84 ??0.84 | ??38324 ??40.02 ??41737 ??43.58 ??95766 ??36298 ??37.9 ??0.75 | ??47647 ??40.38 ??44922 ??38.07 ??1E＋05 ??45569 ??38.62 ??0.94 | ???35331 ???46.59 ???36370 ???47.96 ???75836 ???29454 ???38.84 ????0.7 | ??33858 ??38.1 ??37405 ??42.09 ??88862 ??38260 ??43.06 ??0.67 | 41351.6±5179.7 44.88±5.49 41263.9±3649.5 44.95±5.57 92908.7±13056.1 34483.9±5816.0 37.31±5.07 |
10 batches of red rooted salvia relative retention time statistics of table 2
| Lot number has fingerprint peaks | ????1 | ????2 | ????3 | ????4 | ????5 | ????6 | ????7 | ????8 | ????9 | ????10 | 11(S) | ????12 |
| Material 101 materials 301 materials 502 materials 701 materials 0801 material 0902 material 1001 materials 1101 materials 1102 materials 1103 | ??0.183 ??0.181 ??0.185 ??0.184 ??0.183 ??0.181 ??0.187 ??0.187 ??0.199 ??0.188 | ??0.206 ??0.202 ??0.206 ??0.201 ??0.204 ??0.201 ??0.208 ??0.207 ??0.216 ??0.208 | ??0.214 ??0.211 ??0.216 ??0.213 ??0.213 ??0.212 ??0.226 ??0.219 ??0.230 ??0.22 | ??0.264 ??0.282 ??0.264 ??0.266 ??0.263 ??0.265 ??0.269 ??0.268 ??0.272 ??0.267 | ??0.283 ??0.280 ??0.282 ??0.282 ??0.282 ??0.283 ??0.285 ??0.284 ??0.288 ??0.285 | ??0.401 ??0.397 ??0.403 ??0.405 ??0.400 ??0.396 ??0.409 ??0.410 ??0.413 ??0.406 | ??0.422 ??0.420 ??0.422 ??0.422 ??0.421 ??0.420 ??0.439 ??0.423 ??0.427 ??0.423 | ??0.524 ??0.521 ??0.523 ??0.523 ??0.522 ??0.522 ??0.526 ??0.525 ??0.528 ??0.525 | ??0.609 ??0.604 ??0.614 ??0.616 ??0.609 ??0.606 ??0.622 ??0.623 ??0.626 ??0.624 | ??0.686 ??0.681 ??0.684 ??0.681 ??0.683 ??0.687 ??0.684 ??0.670 ??0.688 ??0.689 | ????1 ????1 ????1 ????1 ????1 ????1 ????1 ????1 ????1 ????1 | ??2.583 ??2.639 ??2.569 ??2.558 ??2.601 ??2.644 ??2.545 ??2.556 ??2.528 ??2.536 |
| Average | ??0.186 | ??0.206 | ??0.217 | ??0.266 | ??0.283 | ??0.404 | ??0.424 | ??0.524 | ??0.615 | ??0.682 | ????1 | ??2.576 |
| ????????SD | ??0.00525 | ??0.00446 | ??0.00636 | ??0.00306 | ??0.00222 | ??0.00564 | ??0.00567 | ??0.00213 | ??0.00810 | ??0.00542 | ????0 | ??0.04070 |
| ???????RSD％ | ???2.82 | ???2.17 | ???2.93 | ???1.15 | ???0.78 | ??1.40 | ???1.34 | ???0.41 | ????1.32 | ????0.29 | ????0 | ???1.58 |
Table 3 reappearance experimental result
3.4 finger-print precision test
| Experiment number total fingerprint peaks | ????1 | ????2 | ????3 | ????4 | ????5 | ????6 | ????7 | ????8 | ????9 | ???10 | ???11(S) | ???12 |
| SD relative retention time average RSD% SD peak area average RSD% SD relative peak area % average RSD% | ??0.000 ??0.182 ??0.17 ??392 ??47463 ??0.83 ??0.95 ??45.8 ??2.07 | ??0.001 ??0.204 ??0.44 ??423 ??18613 ??2.27 ??0.47 ??18.0 ??2.60 | ??0.000 ??0.213 ??0.16 ??141 ??6213 ??2.27 ??0.40 ??17.7 ??2.25 | ??0.001 ??0.265 ??0.23 ??968 ??5051 ??19.16 ??0.90 ??4.9 ??18.53 | ??0.002 ??0.284 ??0.54 ??6762 ??16305 ??41.47 ??6.46 ??15.7 ??41.19 | ??0.001 ??0.399 ??0.27 ??465 ??2493 ??18.66 ??0.44 ??2.4 ??18.43 | ??0.001 ??0.422 ??0.35 ??565 ??12469 ??4.53 ??0.53 ??12.0 ??4.44 | ??0.002 ??0.524 ??0.30 ??932 ??42484 ??2.19 ??1.02 ??41.0 ??2.49 | ??0.001 ??0.608 ??0.18 ??142 ??9552 ??1.49 ??0.23 ??9.2 ??2.52 | ??0.002 ??0.688 ??0.25 ??193 ??2016 ??9.55 ??0.21 ??1.9 ??10.92 | ????0.000 ????1.000 ????0.00 ????2363 ????103668 ????2.28 ????0.00 ????100.0 ????0.00 | ??0.019 ??2.625 ??0.71 ??111 ??35183 ??0.32 ??0.85 ??34.0 ??2.50 |
According to the operation of finger-print precision prescriptive procedure, be test sample with 0801 batch of Shanglou red rooted salvia, get 1 part, continuous sample introduction 5 times is added up the relative retention time at total peak and the peak area that relative peak area has regulation respectively, and RSD% is no more than 3%.3.5 finger-print stability test
According to finger-print 4stability determination operation, be test sample with 0801 batch of Shanglou red rooted salvia, investigates 24 hours solution-stabilized, respectively relative retention time that has the peak and the peak area that relative peak area has regulation are added up, RSD% is no more than 3%.Test sample was stablized in 24 hours.
More than test shows that this assay method is reliable and stable.By the base being implemented the GAP management, links such as the chemical fertilizer of keeping under strict control, agricultural chemicals, the processing of gathering make the red sage root quality controllable, and finger-print is relatively stable.
Further provide the comparison of Shanglou red sage root finger-print and commercially available red rooted salvia collection of illustrative plates below, but the present invention is not limited.
Accompanying drawing and drawing explanation
Fig. 1 reference substance collection of illustrative plates.1---danshensu reference substance 2---protocatechualdehyde reference substance (object of reference)
Fig. 2 Shanglou red sage root finger-print (0～90 minute)
The commercially available red sage root finger-print of Fig. 3 (0～90 minute)
Fig. 4 Shanglou red sage root finger-print (0～30 minute)
The commercially available red sage root of Fig. 5 Shanglou red sage root finger-print (0～30 minute)
The assay method of commercially available red rooted salvia is with Shanglou red sage root finger print measuring method, and the sampling time is 90 minutes, measures 3 batches. See Table 4 with the difference of Shanglou red sage root, chromatogram is seen Fig. 1～5.
The commercially available red sage root of table 4 and the difference of Shanglou red sage root
| Total fingerprint peaks | Peak 1 | Peak 3 | Peak 4 | Peak 5 | Peak 8 | Peak 11 (S) | ||
| City's retention time is sold the red peak area ginseng of relative retention time-RT relative peak area | 4.63 0.179 7037 23.44 | 5.663 0.218 35304 117.58 | Do not have | Do not have | 13.487 0.520 30861 102.78 | 14.08 0.543 18890 62.91 | 25.93 1.000 30026 100.00 | 85.63 3.302 21686 72.22 |
| The merchant's retention time Lip river relative retention time-red peak area ginseng of RT Relative Peak face | 4.600 0.181 42680 52.17 | 5.37 0.211 18550 22.67 | 6.667 0.262 5526 6.75 | 7.143 0.280 18422 22.52 | 13.26 0.521 45282 55.35 | Do not have | 25.473 1.000 81812 100.00 | Do not have |
The result shows the total peak 4 (RT0.264) of shortage in its chromatogram of the commercially available red sage root, 5 (RT0.283); The protocatechualdehyde peak area is lower than standard diagram 50%; With the total peak 1 (RT 0.183) of Shanglou red sage root standard finger-print, 3 (RT0.214), 8 (RT0.524) relative peak area larger difference is arranged in the commercially available red sage root finger-print; Its non-total peak accounts for the gross area and surpasses 10%. The two finger-print has bigger difference, can not the phase trans-substitution.
Claims (7)
1. the method set up of a red rooted salvia finger-print is characterized in that it is to take following steps:
The preparation of step 1 need testing solution: the red rooted salvia powder of getting 30 mesh sieves adds water, and precision is weighed; Refluxing extraction 1-3 hour, put and be chilled to room temperature, supply dehydration; Get extract and put on a small quantity in the measuring bottle, methanol constant volume is to scale, and is centrifugal, gets supernatant as need testing solution;
The preparation of step 2 reference substance solution: it is an amount of to get reference substance, with the moving phase dilution, makes reference substance solution;
Step 3 is measured: each injects liquid chromatograph respectively accurate respectively absorption reference substance solution and need testing solution, according to high effective liquid chromatography for measuring, with the relative retention time of the chromatographic peak of reference substance and peak area is 1 to calculate relative retention time and relative peak area, promptly obtains red sage root standard finger-print.
2, the method for setting up according to the said red rooted salvia finger-print of claim 1, it is characterized in that the chromatographic condition in the step 3 is: chromatographic column is that octadecylsilane chemically bonded silica is a filler; Moving phase is methanol-water-organic acid potpourri; Detect wavelength 254 ± 1nm; Theoretical cam curve is calculated with the danshensu peak, is not less than 2500;
Need testing solution prepares concrete operations and is in the step 1: got 30 mesh sieve red rooted salvia powder 4.00g, and added water 50ml, precision is weighed, refluxing extraction 2 hours is put and is chilled to room temperature, supplies dehydration, extract 2ml puts the 25ml measuring bottle, and methanol constant volume is to scale, and the centrifuging and taking supernatant is as need testing solution;
The preparation concrete operations of step 2 reference substance solution are: get Sodium Danshensu, the protocatechualdehyde reference substance is an amount of, with the moving phase dilution, make and contain Sodium Danshensu 20 μ g, protocatechualdehyde 5.0 μ g, product solution in contrast among every 1ml;
Concrete operations are in the step 3: accurate respectively reference substance solution and need testing solution each 20 μ l, 10 μ l of drawing, inject liquid chromatograph respectively, according to high effective liquid chromatography for measuring, with the relative retention time of the chromatographic peak of protocatechualdehyde and peak area is 1 to calculate relative retention time and relative peak area, promptly obtains red sage root standard fingerprint image.
3, the method for setting up according to the said red rooted salvia finger-print of claim 2 is characterized in that said moving phase is methanol-water-glacial acetic acid of 8: 92: 1.
4, a kind of HPLC finger-print of red rooted salvia is characterized in that it is the HPLC collection of illustrative plates that obtains by the said method of claim 3 of water-soluble extract of red sage root.
5, the HPLC finger-print of red rooted salvia according to claim 4 is characterized in that Shanglou red rooted salvia finger-print, and its total peak is no less than 8.
6,, it is characterized in that by 10 batches of Shanglou red rooted salvia determining fingerprint patterns relatively its chromatogram determines that total peak is 12 according to the HPLC finger-print of the described red rooted salvia of claim 4.
7, the HPLC finger-print of the described red rooted salvia of claim 6, the relative standard deviation RSD of 12 total peak relative retention times that it is characterized in that said Shanglou red sage root is all less than 3%.Wherein, No. 1 average relative retention time RT0.186 in peak (relative standard deviation RSD2.82%), No. 2 peak RT0.206 (RSD2.17%), No. 3 peak RT0.217 (RSD2.93%), No. 4 peak RT0.266 (RSD1.15%), No. 5 peak RT0.283 (RSD0.78%), No. 6 peak RT0.404 (RSD1.40%), No. 7 peak RT0.424 (RSD1.34%), No. 8 peak danshensu RT0.524 (RSD0.41%), No. 9 peak RT0.615 (RSD1.32%), No. 10 peak RT0.682 (RSD0.79%), No. 11 peak protocatechualdehyde RT1.000 (this peak is with reference to the peak), No. 12 peak RT2.576 (RSD1.58%); The fingerprint peaks that wherein surpasses total peak area 10% is No. 1 peak (relative peak area 38.10%～52.17%), No. 8 peaks (danshensu) (relative peak area 38.07～47.96%), No. 11 peaks (protocatechualdehyde) (relative peak area is 1) and No. 12 peaks (relative peak area 28.84%～44.10%).
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