CN1322609A - Gene chip micro lattice operation platform - Google Patents
Gene chip micro lattice operation platform Download PDFInfo
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- CN1322609A CN1322609A CN 01116079 CN01116079A CN1322609A CN 1322609 A CN1322609 A CN 1322609A CN 01116079 CN01116079 CN 01116079 CN 01116079 A CN01116079 A CN 01116079A CN 1322609 A CN1322609 A CN 1322609A
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Abstract
The present invention relates to the preparation of micro lattice chip. The gene chip micro lattice operating platform has three electric transition stages combined into robot with single arm and double arm and one 18-pin sample applying head. Under the control of computer software, electric control box and robot are operated to realize automatic sample application to form complete gene chip. The operating platform has the features of simple operation, high efficiency, high accuracy and low cost and is suitable for scientific research and application in the basic level.
Description
The present invention relates to the preparation of microarray chip.
The gene microarray chip is modal a kind of in the biochip, it can be analyzed thousands of genes simultaneously, the making of gene microarray chip mainly contains two big classes: the one, and the mode of employing direct synthetic oligonucleotide on the chip dot matrix, utilize tabula rasa printing technology fixed point exposure dot matrix in the semi-conductor industry as Affymetrix company, using the solid phase Actinochemical synthesis, parallel synthetic highdensity oligonucleotides dot matrix.The 2nd, adopt dot matrix or ink jet type print process with minim DNA solution directly the array format point put and immobilization on chip carrier with preparation DNA microarray chip.
Because most DNA microarray chip all is to adopt the preparation of the second class manufacture craft, so the development abroad of Microarray instrument is very fast, existing a lot of offshore companies have released the product with own characteristic.External like product technical characterstic mainly is divided three classes: Piezoelectric Printing Technology (piezoelectricity printing technology), Syringe-Solenoid Printing Technology (syringe-solenoid printing technology) and Pin Printing Technology (some pin printing technology).The piezoelectricity printing technology utilizes a piezo-electric crystal (as ceramic material) to link to each other with fluid reservoir, and fluid reservoir is made by capillary glass tube, and the liquid dna sample can be stored in its inside.After adding voltage, cause the distortion of crystal, thereby the extruding capillary ejects a droplets of liquid from the tip.Syringe one solenoid printing technology, the similar syringe that passes through a high precision of principle and syringe extrudes fluid sample.More than two kinds of technology all belong to the off-contact printing technology, promptly needle point does not directly contact with solid dielectric during point sample, and some pin printing technology belongs to the contact printing technology, needle point directly contacts sample spot on medium with solid dielectric during point sample.Point pin printing technology is most widely used a kind of technology, and spotting needle commonly used has Solid Pin (solid needle), Split Pin (slit needle), Tweezer, Micro Spotting Pin etc.
Above-mentioned apparatus expensive, the consumables cost height is not suitable for promoting at home, and mostly be with probe points on slide, the device of a nylon membrane is seldom arranged.
The object of the invention provides a kind of gene chip micro lattice operation platform, can overcome the deficiency of above-mentioned technology, the present invention has that cost is low, efficient is high, accurate, simple to operate, prevent that characteristics such as cross pollution from can extensively promote biochip technology at home.
The present invention includes three-dimensional mechanical hand, sampling head, electric cabinet, computer formation that three electronic control translation stages are formed, manipulator has single armed and both arms, has the sampling head of 1-18 pin simultaneously, operation electric cabinet and manipulator under control of computer software.Three-dimensional mechanical hand has X, Y and Z axle.
The present invention is described in detail as follows in conjunction with the accompanying drawings:
Fig. 1 is a gene chip micro lattice operation platform schematic diagram of the present invention; Fig. 2 is the vertical view of punch block; Fig. 3 is the generalized section of spotting needle; Fig. 4 is the arrangement profile of spotting needle on punch block.
As shown in the figure, 1 is spotting arm, make for lucite, and can be more than one; 2 is accurate translation stage, is one dimension precise manipulation device; 3 is the point sample headstock, and lucite is made, and is used to load punch block; 4 is punch block; 5 is accurate electronic control translation stage Z axle; 6 is accurate electronic control translation stage Y-axis; 7 is accurate electronic control translation stage X-axis; 8 is optical table; 9 is electric cabinet; 10 is computer; 11 for placing the passage of spotting needle, totally 3 * 6=18; 12 is reservoir compartment; 13 is the point sample pinhead wall; 14 is sample channel; 15 is the spotting needle overcoat; 16 is the spotting needle sample export.
Accurate electronic control translation stage X-axis 7 is fixed to and is optical table 8, accurate electronic control translation stage Y-axis 6 is connected to accurate electronic control translation stage X-axis 7, accurate electronic control translation stage Z axle 5 is connected to accurate electronic control translation stage Y-axis 6, spotting arm 1 is connected on the accurate electronic control translation stage Z axle 5, accurate translation stage 2 is connected on the spotting arm 1, the point sample headstock 3 is connected on the accurate translation stage 2, punch block 4 is placed on the point sample headstock 3, spotting needle is placed on the passage 11 of placement spotting needle of punch block 4, with data wire respectively accurate electronic control translation stage Z axle 5, accurate electronic control translation stage Y-axis 6 and accurate electronic control translation stage X-axis 7 are connected on the electric cabinet, at last electric cabinet 9 are connected on the computer 10.
Using method: nylon membrane is fixed on the glass plate, glass plate installs on the optical table 8, open electric cabinet 9 power supplys, start computer 10 and open electric cabinet control software, accurate electronic control translation stage Z axle 5 is made zero, adjust the height of the point sample headstock 3 with accurate translation stage 2, sample is inserted in the reservoir compartment of spotting needle, then spotting needle is placed on the passage 11 of placement spotting needle of punch block 4, the design spotting template is also carried out and is carried out point sample, after one group of sample point sample is finished, take off punch block 4, change and next group sample punch block is housed carries out point sample up to the point sample of finishing all samples.
Spotting needle top is that reservoir compartment 12 bottoms are that the spotting needle neck is made of a wall 13 and sample channel 14, and the spotting needle neck is equipped with spotting needle overcoat 15 outward, and spotting needle sample export 16 is designed to taper.
The making of spotting needle can be selected following parameters for use:
Spotting needle top is that a reservoir compartment 12 can be stored sample 5-100 microlitre, sample channel 14 diameters are 0.1-0.17mm, and length is 1.2-1.7mm, and external diameter is 0.5mm, the spotting needle overcoat 15 that it is 2mm that spotting needle also is with a diameter outward, spotting needle sample export 16 is designed to taper.
Computer control software design a model be control three mechanical arms, control point number with can on identical point, repeat.
Mechanical part minimum resolution of the present invention is 2.5 microns.Spotting needle adopts single needle or spininess point sample, and the point sample volume that can select each sampling point for use is that 50-100 receives that to rise diameter be the 200-1000 micron, and the distance between 2 is the 400-1500 micron, and point sample density is 40-225 point/cm
2
The invention has the advantages that:
(1) cost of instrument itself is low, and an external cover instrument price is generally the hundreds of thousands dollar, and this model machine cost only Be several ten thousand RMB; An external spotting needle price is at the hundreds of dollars, and our spotting needle price only is 1 yuan of people About people's coin; Employed nylon membrane also only has about 1.7 yuan one, and an external slide needs the 1-20 dollar.
(2) adopt symmetrical structure to carry out simultaneously point sample of both arms, make the point sample effective area increase 1 times, go out 20 original * 30cm2Become two 20 * 30cm2, improved 1 times with regard to the efficient that makes machine like this, also strengthened the stable of machine simultaneously The property.
(3) adopt whole plate to change the pin mode and carry out point sample and can effectively prevent cross pollution between the sample, and improve point sample speed.
Point sample instrument of the present invention has that cost is low, efficient is high, accurate, simple to operate, prevent that cross pollution is convenient to characteristics such as popularization.
Claims (6)
1, a kind of gene chip micro lattice operation platform is characterized in that it comprises that mainly three-dimensional mechanical hand, spotting arm, sampling head, spotting needle, electric cabinet and the computer of electronic control translation stage composition constitute, operation electric cabinet and manipulator under computer control; Three-dimensional mechanical hand has X, Y and Z axle;
Accurate electronic control translation stage X-axis is fixed on the optical table, accurate electronic control translation stage Y-axis connects accurate electronic control translation stage X-axis, accurate electronic control translation stage Z axle connects accurate electronic control translation stage Y-axis, spotting arm links to each other with accurate electronic control translation stage Z axle, and accurate translation stage connects spotting arm, and the point sample headstock connects accurate translation stage, punch block is placed on the point sample headstock, spotting needle is placed on the passage of punch block, respectively accurate electronic control translation stage X, Y, Z axle is connected electric cabinet with data wire, and electric cabinet connects computer.
2,, it is characterized in that having 1-18 spotting needle on the said sampling head according to the said gene chip micro lattice operation platform of claim 1.
3, according to the said gene chip micro lattice operation platform of claim 1, it is characterized in that said spotting needle top is that the reservoir compartment bottom is that the spotting needle neck is made of pin wall and sample channel, the spotting needle neck is equipped with the spotting needle overcoat outward, and the spotting needle sample export is designed to taper.
4,, it is characterized in that said spotting arm is single armed or both arms according to the said gene chip micro lattice operation platform of claim 1.
5,, it is characterized in that movement of sample is fixed against X, Y, Z axle that computer is handled according to the said gene chip micro lattice operation platform of claim 1.
6, according to the said gene chip micro lattice operation platform of claim 1, it is characterized in that designing a model of said computer-controlled program be control three mechanical arms, control point number with can on identical point, repeat.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01116079 CN1322609A (en) | 2001-05-15 | 2001-05-15 | Gene chip micro lattice operation platform |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01116079 CN1322609A (en) | 2001-05-15 | 2001-05-15 | Gene chip micro lattice operation platform |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1322609A true CN1322609A (en) | 2001-11-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01116079 Pending CN1322609A (en) | 2001-05-15 | 2001-05-15 | Gene chip micro lattice operation platform |
Country Status (1)
| Country | Link |
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| CN (1) | CN1322609A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1306042C (en) * | 2003-09-01 | 2007-03-21 | 精工爱普生株式会社 | Device and method for manufacturing bead array, and method for detecting target substance |
| CN101205518B (en) * | 2007-11-07 | 2011-05-25 | 汤建新 | Mechanical device for in-situ synthesis of gene chip and system equipment |
| TWI641833B (en) * | 2018-02-07 | 2018-11-21 | 國立臺灣師範大學 | Marking equipment for making biochips |
| CN113189357A (en) * | 2021-05-06 | 2021-07-30 | 中国科学院上海微系统与信息技术研究所 | Preparation method of dipping type sample applicator and micro-cantilever sensor chip |
-
2001
- 2001-05-15 CN CN 01116079 patent/CN1322609A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1306042C (en) * | 2003-09-01 | 2007-03-21 | 精工爱普生株式会社 | Device and method for manufacturing bead array, and method for detecting target substance |
| CN101205518B (en) * | 2007-11-07 | 2011-05-25 | 汤建新 | Mechanical device for in-situ synthesis of gene chip and system equipment |
| TWI641833B (en) * | 2018-02-07 | 2018-11-21 | 國立臺灣師範大學 | Marking equipment for making biochips |
| CN113189357A (en) * | 2021-05-06 | 2021-07-30 | 中国科学院上海微系统与信息技术研究所 | Preparation method of dipping type sample applicator and micro-cantilever sensor chip |
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