CN1321516A - Multistrain universal inactivated vaccine for bovine, ovine and porcine foot-and-mouth diseases - Google Patents
Multistrain universal inactivated vaccine for bovine, ovine and porcine foot-and-mouth diseases Download PDFInfo
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- CN1321516A CN1321516A CN 00113804 CN00113804A CN1321516A CN 1321516 A CN1321516 A CN 1321516A CN 00113804 CN00113804 CN 00113804 CN 00113804 A CN00113804 A CN 00113804A CN 1321516 A CN1321516 A CN 1321516A
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- foot
- mouth disease
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Abstract
The present invention relates to a multitoxistrain universal inactivated vaccine for preventing bovine, sheep and swine foot-and-mouth disease. Said vaccine is made up by using O type toxistrains of at least three kinds of foot-and-mouth disease viruses, emulsifier and medicinal white oil through the conventional vaccine preparation process. Said invented vaccine is prepared by using several toxistrains of bovine, sheep and swine, so its antigen content can be raised, its antigenicity is good, and its immunizing potency is high.
Description
The present invention relates to a kind of cattle, sheep, many strains of Schweineseuche universal inactivated vaccine.
Foot and mouth disease is multiple domestic animal deadly infectious diseases such as artiodactyl cattle, sheep, pig.Because its popular animal husbandry and national economy of often giving is brought heavy losses, be called " political economy disease ", classified as category-A infectious disease number one by International Office of Epizootics.National governments all attach great importance to the preventing and controlling of primary disease.
Vaccinate is one of important means of control primary disease.The existing foot and mouth disease inactivated vaccine of China divides cattle, sheep to use and pig is used two classes.Use single strain seedling respectively, it is antigenic representative not enough, and the antigen amount is few, and immune efficacy is lower.And versatility is relatively poor, the hoof-and-mouth disease that cattle, sheep, pig are sent out altogether preventative relatively poor.
The objective of the invention is to have improved antigenic content for a kind of utilize cattle, sheep, a plurality of strain mixing of pig seedling are provided, the antigenicity of mixing seedling is good, immune efficacy is high, can be suitable for cattle, sheep, the general many strains inactivated vaccine of pig.
Purpose of the present invention can realize by following measure:
A kind of cattle, sheep, many strains of Schweineseuche universal inactivated vaccine are to be made through conventional vaccine preparing process preparation by the O type strain of at least three kinds of foot and mouth disease viruses, and it is made by following raw materials in percentage by weight:
Foot and mouth disease virus (Aphthae Eizooticae Aviariae) OA58 20-30%
Foot and mouth disease virus (Aphthae Eizooticae Aviariae) OMII 20-30%
Foot and mouth disease virus (Aphthae Eizooticae Aviariae) OY-80 20-30%
Emulsifying agent 3-8% medicinal white oil 22-32%.Described emulsifying agent is a tween 80, Si Ben-80.
Above-mentioned foot and mouth disease virus OA58, OM II, OY-80 provide by the Ministry of Agriculture's specified Lanzhou veterinary institute.The commercial disignation of OA58 is AV97, is isolated from 58 years foot and mouth disease viruses of Aksu, Xinjiang cattle; The commercial disignation of OM II is AV100, is the Mus removing toxic substances of foot and mouth disease; OY-80 itself is exactly a commercial disignation, separates from Guangxi melt water Schweineseuche virus.OA58 and OM II are write " Chinese veterinary's strain catalogue " of publishing in 1992 141,142 pages and are gone up and disclose its title and compositions at the supervisory institute of Chinese veterinary drug, Chinese microbial preservation administration committee veterinary microorganism center.OY-80 discloses its title and composition for the 20th page at Ministry of Agriculture's veterinary biological product quality standard disclosed " People's Republic of China's veterinary biologics quality standard ".
Kind of the present invention poison cultivate be with three kinds of strains respectively with the cultivation of going down to posterity of BHK-21 passage cell, its incubation that goes down to posterity is as follows:
1, plant cell: produce and use the BHK-21 passage cell, China Veterinary Drugs Supervisory Inst. seventies is by external introducing.Planting cell is stored in the liquid chlorine.Cell is suspended in the nutritional solution after thawing and recovering.
Cell nutrient solution:
The E-MEM culture medium
0.5% lactoalbumin hydrolysate Euclidean liquid
(above two kinds of solution mixed in equal amounts)
Calf serum 5-10%
7.5% sodium bicarbonate is transferred PH7.4
100,000 every milliliter of cell concentrations cultivate that to grow up to fine and close monolayer in 48 hours standby for 36-37 ℃.
2, cell enlarges:
Cell carries out suitability for industrialized production with 15 liter garden bottle rotating and culturing at last through the several amplification culture.
3, virus inoculation:
Well-grown cell is discarded nutritional solution inoculate the liquid of keeping that contains different strain viruses respectively and continue to cultivate, keeping liquid is 0.5%E-LH solution, does not contain MEM and Ox blood serum, and PH7.6 produces pathological changes, comes off through the 10-20 hour cell, can gather in the crops.Virus inoculation is in the BHK21 cell monolayer, and successive transfer culture is more than 12 generations continuously, and the 6th generation began to measure results liquid poison valency all more than 8.0 with the neonatal rat method, and wherein the 8th generation rose and reaches LD
608.25-9.0.
4, inactivation of virus:
Virus BEI deactivation is used to join Seedling after with neonatal rat and zoometry deactivation fully.
The present invention has following advantage compared to existing technology:
1, the present invention utilizes a plurality of strain mixing seedlings of cattle, sheep, pig, has improved antigenic content, and antigenicity is good, immune efficacy is high.
Immunogenicity determining of the present invention: with the cell harvesting liquid seedling of virus inoculation, immune cattle carries out challenge test, and counteracting toxic substances is tested three batches of each 5 cattle with 10000 morbidity amounts of epidemic isolates, always protects 15/15.Not immunity contrast cattle 3/3 morbidity.
Vaccine performance measurement of the present invention:
Minimum immune quantitative determination:
Vaccine 3 batches (9901,9902,9903) is pressed the i.e. 1.0 milliliters of immune cattles of consumption 1/3, attacks with the strong poison of 10000 total morbidity amounts in 30 days, protects 4/5,4/5,4/5 respectively for three batches, and contrast 3/3 is fallen ill, and illustrates that 1/3 dosage can reach Ministry of Agriculture's quality standard promulgated by the ministries or commissions of the Central Government.
The vaccine potency check:
Imitate 20 batches of inspections in accordance with regulations, 5 of the every batch of immune cattles, 3 milliliters of immunity amounts, the counteracting toxic substances amount contains 10000 total morbidity amounts for 2.0 milliliters, and immune cattle is all protected, and contrasts cattle and all falls ill, and stipulates that promptly the counteracting toxic substances amount can protect 100%.
To the pig potency test:
9901 batches, 9905 batches, 9919 batches of vaccines are 5 of immune swines respectively, and back 28 days of immunity is attacked with pig source strength poison OY80, and equal 5/5 protection illustrates the pig protection good.
The immune cattle serum antibody is measured:
| The vaccine lot number | ??9901 | ??9903 | ??9905 | ??9910 | ??9915 | ??9919 |
| ??PD50 | ?1∶354 | ?1∶316 | ?1∶354 | ?1∶354 | ?1∶316 | ?1∶354 |
Introducing PD50 according to data reaches and gets final product 100% more than 1: 128 and resist strong virus attack.Can find out that from The above results vaccine has good immune effect.
2, nontoxic, the safety of vaccine of the present invention.
The present invention also will be described in further detail in conjunction with the embodiments:
Embodiment 1: a kind of cattle, sheep, many strains of Schweineseuche universal inactivated vaccine are formulated through conventional vaccine preparing process by following raw materials in percentage by weight: foot and mouth disease virus OA58 20%, foot and mouth disease virus OM II 20%, foot and mouth disease virus OY-880 30%, tween 80 6%, medicinal white oil 24%.
Embodiment 2:
A kind of cattle, sheep, many strains of Schweineseuche universal inactivated vaccine are formulated through conventional vaccine preparing process by following raw materials in percentage by weight: foot and mouth disease virus OA58 25%, foot and mouth disease virus OM II 25%, foot and mouth disease virus OY-80 20%, Si Ben-80 3%, medicinal white oil 27%.
Embodiment 3:
A kind of cattle, sheep, many strains of Schweineseuche universal inactivated vaccine are formulated through conventional vaccine preparing process by following raw materials in percentage by weight: foot and mouth disease virus OA58 30%, foot and mouth disease virus OM II 20%, foot and mouth disease virus OY-80 25%, Si Ben-80 3%, medicinal white oil 22%.
Claims (1)
1, a kind of cattle, sheep, many strains of Schweineseuche universal inactivated vaccine is characterized in that this vaccine is formulated through conventional vaccine preparing process by the O type strain of at least three kinds of foot and mouth disease viruses, and it contains raw material by the following weight percentage and makes:
Foot and mouth disease virus (Aphthae Eizooticae Aviariae) OA58 20-30%
Foot and mouth disease virus (Aphthae Eizooticae Aviariae) OM II 20-30%
Foot and mouth disease virus (Aphthae Eizooticae Aviariae) OY-80 20-30%
Emulsifying agent 3-8% medicinal white oil 22-32%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00113804 CN1321516A (en) | 2000-04-28 | 2000-04-28 | Multistrain universal inactivated vaccine for bovine, ovine and porcine foot-and-mouth diseases |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00113804 CN1321516A (en) | 2000-04-28 | 2000-04-28 | Multistrain universal inactivated vaccine for bovine, ovine and porcine foot-and-mouth diseases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1321516A true CN1321516A (en) | 2001-11-14 |
Family
ID=4583559
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00113804 Pending CN1321516A (en) | 2000-04-28 | 2000-04-28 | Multistrain universal inactivated vaccine for bovine, ovine and porcine foot-and-mouth diseases |
Country Status (1)
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101565458A (en) * | 2009-06-04 | 2009-10-28 | 中牧实业股份有限公司 | Peptide vaccine for animal and preparation method thereof |
| CN102335426A (en) * | 2011-10-30 | 2012-02-01 | 张大宇 | Traditional Chinese medicine animal health-care product for foot-and-mouth disease of cattle |
| CN103848902A (en) * | 2014-03-07 | 2014-06-11 | 中牧实业股份有限公司 | Synthetic peptide vaccine and preparation method thereof |
-
2000
- 2000-04-28 CN CN 00113804 patent/CN1321516A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101565458A (en) * | 2009-06-04 | 2009-10-28 | 中牧实业股份有限公司 | Peptide vaccine for animal and preparation method thereof |
| CN101565458B (en) * | 2009-06-04 | 2014-01-08 | 中牧实业股份有限公司 | Peptide vaccine for animal and preparation method thereof |
| CN103936841B (en) * | 2009-06-04 | 2016-01-20 | 中牧实业股份有限公司 | A kind of peptide vaccine for animal and preparation thereof |
| CN102335426A (en) * | 2011-10-30 | 2012-02-01 | 张大宇 | Traditional Chinese medicine animal health-care product for foot-and-mouth disease of cattle |
| CN103848902A (en) * | 2014-03-07 | 2014-06-11 | 中牧实业股份有限公司 | Synthetic peptide vaccine and preparation method thereof |
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