CN1319327A - Young banian in vitro quick propagation method - Google Patents
Young banian in vitro quick propagation method Download PDFInfo
- Publication number
- CN1319327A CN1319327A CN 01107615 CN01107615A CN1319327A CN 1319327 A CN1319327 A CN 1319327A CN 01107615 CN01107615 CN 01107615 CN 01107615 A CN01107615 A CN 01107615A CN 1319327 A CN1319327 A CN 1319327A
- Authority
- CN
- China
- Prior art keywords
- bud
- young
- banian
- consumption
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention discloses an isolated quick propagation method of Young banian, and its technical key lies in that axiallry bud of Young banian is used as explant, induced to produce fascilated bud, cut into single bud, inoculated into solid culture medium to make subculture to obtain lots of fasciculated bud, transplanting chit into rooting culture medium and inducing to root so as to obtain lots of strong health plants and taking out from bottle to make transplantation. It said invention solid culture method is utilized to raise coefficient of chit propagation, shorten culture time and ensure quality of plant.
Description
The present invention relates to method for plant tissue culture, particularly a kind of with solid culture to improve the method for little banyan reproduction speed.
China is along with reform and opening-up, economy develops rapidly, people's living standard improves, in Beijing, the popular ornamental waterweed heat in ground such as Shanghai, Guangzhou and Shenzhen, the pasture and water of the various form graces of plantation in glass water tank, mix colorful tropical fish and cruise wherein, arrange indoor environment pure and fresh more quiet and tastefully laid outly, splendid.
Little banyan (Anubias barteri nana) is the ornamental small-sized pasture and water that belong to the water banyan class, originates in West Africa, and plant height has only 10cm at most, and blade is bottle green, and is avette.Water banyan is the characteristic pasture and water in African tropical waters, makes scape with water banyan class pasture and water, can reflect the view in tropical waters.Little banyan is a kind of of water banyan, and the present demand of China mainly is to be introduced by Taiwan, Japan and Singapore and other places, and conventional modes of reproduction mainly is the side shoot breeding that grows with rhizome, and reproduction coefficient is low, and restricted by environment and season, can not meet the need of market.Adopting method for plant tissue culture to breed little banyan is one of method that obtains a large amount of best buy seedlings, yet there are no the report of relevant Young banian in vitro quick propagation method at present at home.
Purpose of the present invention just provides the method that a kind of Young banian in vitro easy and simple to handle, that reproduction speed is fast is bred.
The present invention can be realized by following mode: the axillalry bud with little banyan is made explant, induce and produce the bud of growing thickly, be cut into simple bud, be seeded in and make successive transfer culture in the solid culture medium, obtain a large amount of buds of growing thickly, change young shoot in root media root induction, obtain a large amount of healthy and strong whole plants and bottle outlet and transplant.At first, induce little banyan resting bud to sprout and make explant, the finished product seedling that cuts terminal bud is partly soaked shape be positioned over minimal medium based on MS, by preparation 1L medium macroelement consumption be original MS 1/10～1/5, trace element and molysite consumption be original MS consumption 1/5～1/2 and contain in the nutrient solution that 0.1～0.5mg/L6-BA forms, after 20 days, almost the resting bud under each axil all grows up to young shoot, after bud is downcut routinely disinfection way and handles, obtains sterilizable material and makes explant; Secondly, explant is seeded on the medium of MS+6-BA1.0mg/L+IAA0.3mg/L (following unit is identical, omits), agar 0.75%, white sugar 3%, PH5.8 and makes initial incubation, induces to produce the bud of growing thickly; Moreover the bud of will growing thickly is cut into simple bud, is seeded in the medium of 1/3MS+6-BA2.0mg/L+IAA0.2mg/L, agar 0.75%, white sugar 3%, PH5.8 and makes successive transfer culture, 23～27 ℃ of cultivation temperature, illumination 12hd
-1, illuminance 1500Lux produces grow thickly the in a large number bud and the plant that takes root; The 4th, young shoot is changed over to root induction on the medium of MS+IAA0.4, agar 0.75%, white sugar 3%, PH5.8,23～27 ℃ of cultivation temperature, illumination 12hd
-1, illuminance 1500Lux, incubation time are 20 days; At last, plant is planted in fine sand makes matrix and be added with to do transition in the basin of the water purification that floods the sand face and cultivate, can be planted in after 1 month and do in the mud further to cultivate.1/3MS is meant preparation 1L medium based on the MS minimal medium, and the macroelement consumption is 1/3 of an original MS consumption, and trace element, molysite and organic matter (the inositol consumption is constant) consumption are 1/2 of original MS consumptions.
The present invention had both improved the young shoot reproduction coefficient by solid culture method, had shortened incubation time, had guaranteed the quality of plant again, provided new approach for accelerating little banyan high-quality seedling reproduction speed.
Below in conjunction with embodiment, the present invention is further illustrated.
Embodiment one
1, induce the sprouting of little banyan resting bud to make explant
The present invention adopts the axillalry bud of little banyan to make explant.Because its axillalry bud is the dormancy shape under the natural conditions, therefore, the finished product seedling that will cut terminal bud among the present invention partly soaks shape and is positioned over the minimal medium based on MS, by preparation 1L medium macroelement consumption be original MS 1/6, trace element and molysite consumption be original MS consumption 1/2 and contain in the nutrient solution that 0.3mg/L6-BA forms, after 20 days, almost the resting bud under each axil all grows up to about 0.2cm height, has the bud of 1～2 leaf.Axillalry bud is downcut, at first, clean with flushing with clean water again with the running water flushing 5min that is added with a small amount of washing powder, under aseptic condition, with 75% alcohol disinfecting 15s, 0.1% mercuric chloride soaks 8min then, use aseptic water washing at last 4～5 times, obtain sterilizable material and make explant.
2, initial incubation
The axillalry bud explant that obtains is seeded on the medium of MS+6-BA1.0mg/L+IAA0.3mg/L, agar 0.75%, white sugar 3%, PH5.8 23～27 ℃ of cultivation temperature, illumination 12hd
-1, illuminance 1500Lux cultivated after 40 days, and the axillalry bud base portion has produced callus lines, and also long above have young shoot, and inductivity reaches about 80%.
3, successive transfer culture
The bud of will growing thickly is cut into simple bud, is seeded on the medium of 1/3MS+6-BA2.0mg/L+IAA40.2mg/L, agar 0.75%, white sugar 3%, PH5.8 23～27 ℃ of cultivation temperature, illumination 12hd
-1, illuminance 1500Lux, cultivation cycle is 60 days, it the results are shown in Table one.1/3MS is meant preparation 1L medium, and based on the MS minimal medium, the macroelement consumption is 1/3 of an original MS consumption, and trace element, molysite and organic matter (the inositol consumption is constant) consumption are 1/2 of original MS consumptions.Table one
Reproduction coefficient=number/inoculation number sprouts
| Medium (mg/L of unit) | Inoculation number (individual) | Total bud number (individual) | Number (individual) sprouts | Reproduction coefficient |
| 1/3MS+6-BA2.0+IAA0.2 agar 0.75%, white sugar 3%, PH5.8 | ???26 | ???131 | ????105 | ????4.04 |
4, culture of rootage
The bud that high 1.0cm is had 2 leaves changes root induction in the medium of MS+IAA0.4mg/L, agar 0.75%, white sugar 2%, PH5.8 over to, and it the results are shown in Table two.Table two
Rooting rate=number/inoculation number * 100% of taking root
| Medium (mg/L of unit) | Incubation time (my god) | Inoculation number (individual) | Take root and count (individual) | Rooting rate (%) | Plant growth condition |
| MS+IAA0.4, agar 0.75%, white sugar 3%, PH5.8, | 20 | 30 | 30 | 100 | It is blackish green glossy that robust plant, leaf are, and average every strain seedling has 4 roots that are about 1.5cm. |
5, bottle outlet is transplanted
Hardening was 3 days before the seedling bottle outlet was transplanted, take out afterwash root medium, be planted in then with fine sand and make matrix and be added with in the basin of the water purification that floods the sand face, under the shady and cool condition of normal temperature, do transition and cultivate, survival rate reaches more than 90% after 1 month, can be planted in to do in the mud further to cultivate.
Show that from above result of the test adopt solid culture medium 1/3MS+6-BA2.0mg/L+IAA0.2mg/L to breed little banyan seedling, cultivation cycle is 60 days, reproduction coefficient is 4.04, if make continuous culture, can cultivate for 6 generations in 1 year, then can produce 4348 of sproutings by 1 bud; And adopt traditional propagation by division method, can only breed about 10 strains in 1 year.Compare with conventional method, the present invention adopts solid culture method, has improved little banyan reproduction speed greatly, and can obtain high-quality seedling in batch at short notice.
Claims (7)
1, a kind of method of Young banian in vitro quick propagation, it is characterized in that the axillalry bud with little banyan is an explant, induce and produce the bud of growing thickly, be cut into simple bud, be seeded in and make successive transfer culture in the solid culture medium, obtain a large amount of buds of growing thickly, change young shoot in root media root induction, obtain a large amount of healthy and strong whole plants and bottle outlet and transplant.
2, the method of Young banian in vitro quick propagation according to claim 1, it is characterized in that inducing little banyan resting bud to sprout and make explant, the little banyan finished product seedling that cuts terminal bud is partly soaked shape be positioned over minimal medium based on MS, preparation 1L medium macroelement consumption is 1/10～1/5 of an original MS consumption, trace element and molysite consumption be original MS consumption 1/5～1/2 and contain in the nutrient solution of 0.1～0.5mg/L6-BA composition, after 20 days, almost the resting bud under each axil all grows up to young shoot, after bud downcut routinely disinfection way and handle, obtain sterilizable material and make explant.
3, the method for Young banian in vitro quick propagation according to claim 1, it is characterized in that explant makes initial incubation on the medium of MS+6-BA1.0mg/L+IAA0.3mg/L, agar 0.75%, white sugar 3%, PH5.8, induce and produce the bud of growing thickly, 23～27 ℃ of cultivation temperature, illumination 12hd
-1, illuminance 1500Lux.
4, the method for Young banian in vitro quick propagation according to claim 1, the bud that it is characterized in that growing thickly is cut into simple bud, be seeded on the medium of 1/3MS+6-BA2.0mg/L+IAA0.2mg/L, agar 0.75%, white sugar 3%, PH5.8 and make successive transfer culture, 23～27 ℃ of cultivation temperature, illumination 12hd
-1, illuminance 1500Lux cultivated after 60 days, produced a large amount of buds of growing thickly.
5, the method for Young banian in vitro quick propagation according to claim 1, it is characterized in that young shoot by the solid culture acquisition, change root induction on the medium of MS+IAA0.4mg/L, agar 0.75%, white sugar 2%, PH5.8 over to, 23～27 ℃ of cultivation temperature, illumination 12hd
-1, illuminance 1500Lux, incubation time is 20 days, obtains whole plant.
6, the method for Young banian in vitro quick propagation according to claim 1 is characterized in that plant is planted in fine sand and makes matrix and be added with to do transition in the basin of the water purification that floods the sand face and cultivate, and can be planted in after 1 month and do in the mud further to cultivate.
7, the method for Young banian in vitro quick propagation according to claim 4, it is characterized in that 1/3MS is meant that preparation 1L medium is based on the MS minimal medium, the macroelement consumption is 1/3 of an original MS consumption, and trace element, molysite and organic matter (the inositol consumption is constant) consumption are 1/2 of original MS consumptions.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011076151A CN1139319C (en) | 2001-02-27 | 2001-02-27 | Young banian in vitro quick propagation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011076151A CN1139319C (en) | 2001-02-27 | 2001-02-27 | Young banian in vitro quick propagation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1319327A true CN1319327A (en) | 2001-10-31 |
| CN1139319C CN1139319C (en) | 2004-02-25 |
Family
ID=4656530
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB011076151A Expired - Fee Related CN1139319C (en) | 2001-02-27 | 2001-02-27 | Young banian in vitro quick propagation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1139319C (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102232360A (en) * | 2011-04-25 | 2011-11-09 | 安徽农业大学 | Method for establishing in-vitro rapid propagation system of anubias barteri var. barteri, anubias barteri var. nana, ficus henryi and anubias hastifolia |
| CN104026011A (en) * | 2014-06-09 | 2014-09-10 | 浙江省萧山棉麻研究所 | Culture medium for tissue culture and quick reproduction of Ficus pumia 'Variegata' and reproduction method |
| CN106359101A (en) * | 2016-10-10 | 2017-02-01 | 广西大学 | Tissue culture and rapid propagation method of ficus deltoidea |
| CN107711518A (en) * | 2017-12-01 | 2018-02-23 | 广州百德园艺有限公司 | The culture medium and rapid propagation method of great Ye banyan tissue-culturing quick-propagations |
-
2001
- 2001-02-27 CN CNB011076151A patent/CN1139319C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102232360A (en) * | 2011-04-25 | 2011-11-09 | 安徽农业大学 | Method for establishing in-vitro rapid propagation system of anubias barteri var. barteri, anubias barteri var. nana, ficus henryi and anubias hastifolia |
| CN102232360B (en) * | 2011-04-25 | 2012-12-12 | 安徽农业大学 | Method for establishing in-vitro rapid propagation system of anubias barteri var. barteri, anubias barteri var. nana, ficus henryi and anubias hastifolia |
| CN104026011A (en) * | 2014-06-09 | 2014-09-10 | 浙江省萧山棉麻研究所 | Culture medium for tissue culture and quick reproduction of Ficus pumia 'Variegata' and reproduction method |
| CN104026011B (en) * | 2014-06-09 | 2016-04-20 | 浙江省萧山棉麻研究所 | The substratum of the tissue-culturing quick-propagation of silver leaf climbing fig and propagation method |
| CN106359101A (en) * | 2016-10-10 | 2017-02-01 | 广西大学 | Tissue culture and rapid propagation method of ficus deltoidea |
| CN107711518A (en) * | 2017-12-01 | 2018-02-23 | 广州百德园艺有限公司 | The culture medium and rapid propagation method of great Ye banyan tissue-culturing quick-propagations |
| CN107711518B (en) * | 2017-12-01 | 2018-12-11 | 广州百德园艺有限公司 | The culture medium and rapid propagation method of great Ye banyan tissue-culturing quick-propagation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1139319C (en) | 2004-02-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1258321C (en) | Quick breeding method for butterfly orchid high quality sprout | |
| CN102696487B (en) | Method for building leaf in vitro regeneration system of begonia rex | |
| CN101647392B (en) | Tissue-culture rapid-propagation method of double-happiness cuckoo variety and special culture medium thereof | |
| CN103518625B (en) | The tissue culture medium (TCM) of Root of Fiddleleaf Fig blade and in-vitro regeneration method | |
| CN102265785A (en) | Tissue culturing method of hemerocallis middendorfii poinsettia | |
| CN100429306C (en) | Tissue culture medium and fast propagation method for Sorbone lily | |
| CN104012417A (en) | High-efficiency and rapid micropropagation method for toxicodendron vernicifluum | |
| CN104663447A (en) | Building method of orostachys malacophyllus tissue culture regeneration system | |
| CN104509439A (en) | Method suitable for tissue rapid cultivation of Acer rubrum | |
| CN101637123B (en) | Organic-culturing quick-reproducing method of curcuma | |
| CN113100060B (en) | Tissue culture propagation method for alpine rhododendron | |
| CN101810144A (en) | Rapid breeding method of senecio cruentus | |
| CN101015280B (en) | Tissue culture method for fast propagation of primula denticulata ssp.sino-denticulata | |
| CN106165648B (en) | A kind of cercis tissue culture culture medium and cultural method | |
| CN103416302B (en) | Method for culturing regeneration plant of somatic embryo of osmanthus fragrans Lour | |
| CN101743908A (en) | Tissue culture, rapid propagation and cultivation method of grevillea banksii | |
| CN1139319C (en) | Young banian in vitro quick propagation method | |
| CN108391591B (en) | Tissue culture and rapid propagation method for tabebuia flavedo | |
| CN101015279B (en) | Tissue culture method for fast propagation of primula poissonii | |
| CN113016610B (en) | Quinoa hypocotyl in-vitro regeneration method | |
| CN101336615A (en) | Matted bamboo tip tissue culture medium and tissue culture and rapid propagation method | |
| CN101133716B (en) | Mondevilla tissue culturing and propagating method | |
| CN106069746B (en) | A kind of method of flat Europe hybrid hazel tissue-culturing rapid propagation nursery | |
| CN110447538A (en) | It is a kind of that rachis is revealed as the method for tissue culture of explant using Pan Shi ice lantern jade | |
| CN1631089A (en) | Culture method of Sinningia speciosa |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |