CN1316513A - Deeply fermented liquid culture medium for dephosphorizing bacteria - Google Patents
Deeply fermented liquid culture medium for dephosphorizing bacteria Download PDFInfo
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- CN1316513A CN1316513A CN 00105567 CN00105567A CN1316513A CN 1316513 A CN1316513 A CN 1316513A CN 00105567 CN00105567 CN 00105567 CN 00105567 A CN00105567 A CN 00105567A CN 1316513 A CN1316513 A CN 1316513A
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- vitamins
- 3ppm
- bacteria
- phosphate
- culture medium
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Abstract
A culture medium for deeply venitilation fermentation to culture dephosphorizing bacteria used to prepare microbic fertilizer, contains soybean sprout juice, cane sugar, sweet potato starch, the salts of K, Na, ammonium and Ca, trace elements and vitamines. Its advantages include balanced nutrients, proper pH value and osmotic pressure, promoting growth and reproduction of phosphorus bacteria, and high activity of bacteria.
Description
The present invention is a kind of phosphate-solubilizing bacteria liquid submerged aerobic fermentation base of producing microbial fertilizer.
The key of microbial fertilizer quality is that institute contains amount of viable bacteria and quality, and the quality and quantity key of bacterium is fermention medium.Phosphate solubilizing bacteria in the existing microbial fertilizer generally adopts extractum carnis, protein culture medium shake flask fermentation, and cost is higher, is not suitable for cultivating in a large number with fermentor tank; With potato, soya-bean cake, peptone etc. is the substratum fermentation, and number of viable is not ideal enough.Gemma forms slowly, also produces autolysis sometimes, causes the fertilizer quality instability.
The invention provides a kind of substratum of forming jointly by crude substance, inorganic salt and VITAMIN, be fit to phosphate-solubilizing bacteria deep liquid aerobic fermentation, bacterial classification is provided by China Committee for Culture Collection of Microorganisms agricultural microorganism center, is bacillus megaterium (Bacillus megatherium) numbering: ACCC10011.The substratum composition has:
Soybean sprout sweat 10% sweet potato flour 2% soybean cake powder 1%
Sucrose 1% ammonium sulfate 0.05% dipotassium hydrogen phosphate 0.03%
Lime carbonate 0.3% sodium-chlor 0.02% yeast extract paste 0.05%
Liquid microelement 0.2%, vitamins B
1The 3ppm vitamins B
123ppm
Add 0.1% soya-bean oil in addition as foam killer.
The present invention is through the production practice of repetition test and a year, and effect is remarkable.
1. various nutrition reasonable ratio of this substratum can guarantee the growth needs of phosphate-solubilizing bacteria, promptly can finish a fermentation period about 20 hours, and the gemma rate of formation is more than 80%.
2. this substratum is raw material with agricultural byproducts, material is abundant, cheap, after only needing once to prepare burden, once sterilizing, any material is no longer added in fermentation midway, can keep the stable of pH value in the whole fermentation process, have save time, the advantage of saving of labor, saving cost, avoided reinforced again midway easily by the phenomenon of living contaminants.
3. adopt the present invention rationally to solve the equilibrium problem between phosphate solubilizing bacteria growth desired nutritional material in the fermentor tank, also solved the metastable problems of growing environment such as a jar interior potential of hydrogen, osmotic pressure and redox potential well.Every milliliter of viable count of nutrient solution can reach hundred million of 20-50, deposited half a year by peat composed of rotten mosses absorption after, every gram still contains hundred million of viable bacteria 2-3.
Below introduce the technology implementation example that the present invention provides:
Embodiment 1: the preparation of soybean sprout juice in the substratum
Take by weighing the soybean sprout that cultivates 1000 grams, add 1000 milliliters in water, boiled 30 minutes, cross leaching juice, moisturizing to 1000 milliliter promptly gets soybean sprout juice.
Embodiment 2: the preparation of liquid microelement in the substratum
Take by weighing manganous sulfate, each 3 gram of ferrous sulfate, be dissolved into respectively in 1000 ml waters, be made into two kind of 0.3% liquid microelement.
Embodiment 3: fermentative medium formula
100 milliliters of soybean sprout juices, sweet potato flour 20 grams, soybean cake powder 10 grams, sucrose 10 grams, yeast extract paste 0.5 gram, ammonium sulfate 0.5 gram, dipotassium hydrogen phosphate 0.3 gram, sodium-chlor 0.2 gram, lime carbonate 3 grams, 2 milliliters of liquid microelements (each 1 milliliter in 0.3% manganous sulfate and 0.3% ferrous sulfate), vitamins B
10.003 gram, vitamins B
120.003 gram.
With above-mentioned composition mixing and water adding to 1000 milliliter, other adds 1 milliliter of soya-bean oil as foam killer, regulates pH value 7.0～7.4, the sterilization back fermention medium.
Claims (4)
1. a phosphate-solubilizing bacteria liquid submerged aerobic fermentation base of producing microbial fertilizer is characterized in that: be made up of soybean sprout juice 10%, sweet potato flour 2%, soybean cake powder 1%, sucrose 1%, yeast extract paste 0.05%, ammonium sulfate 0.05%, dipotassium hydrogen phosphate 0.03%, sodium-chlor 0.02%, lime carbonate 0.3%, liquid microelement 0.2% and VITAMIN.
2. phosphate-solubilizing bacteria according to claim 1 is characterized in that: provided by China Committee for Culture Collection of Microorganisms agricultural microorganism center, belong to bacillus megaterium (Bacillus megatherium), numbering ACCC10011.
3. liquid microelement according to claim 1 is characterized in that: 0.3% manganous sulfate, 0.3% ferrous sulfate mix with volume.
4. VITAMIN according to claim 1 is characterized in that referring to vitamins B
1And vitamins B
12, the concentration in substratum all is 3PPM, that is: vitamins B
13PPM, vitamins B
123PPM.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00105567 CN1316513A (en) | 2000-04-03 | 2000-04-03 | Deeply fermented liquid culture medium for dephosphorizing bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00105567 CN1316513A (en) | 2000-04-03 | 2000-04-03 | Deeply fermented liquid culture medium for dephosphorizing bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1316513A true CN1316513A (en) | 2001-10-10 |
Family
ID=4577783
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00105567 Pending CN1316513A (en) | 2000-04-03 | 2000-04-03 | Deeply fermented liquid culture medium for dephosphorizing bacteria |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1316513A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105255785A (en) * | 2015-11-17 | 2016-01-20 | 山东省农业科学院农业资源与环境研究所 | Fermentation method of bacillus megatherium with high rate of sporation |
| CN113812298A (en) * | 2021-09-17 | 2021-12-21 | 中邦山水规划设计有限公司 | By using phosphate solubilizing bacteria and vitamin B12Method for realizing rapid growth of poplar |
-
2000
- 2000-04-03 CN CN 00105567 patent/CN1316513A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105255785A (en) * | 2015-11-17 | 2016-01-20 | 山东省农业科学院农业资源与环境研究所 | Fermentation method of bacillus megatherium with high rate of sporation |
| CN113812298A (en) * | 2021-09-17 | 2021-12-21 | 中邦山水规划设计有限公司 | By using phosphate solubilizing bacteria and vitamin B12Method for realizing rapid growth of poplar |
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| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| REG | Reference to a national code |
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