CN1312005A - Method for controlling insect pest - Google Patents

Abstract

The present invention provides a method of controlling Ostrinia furnacalis (Asian Corn Borer) species in crop plants, but especially in maize and other cereal plants, including, but not limited to the following species of plants: maize, wheat, barley, rye, oats, rice, sorghum, millet and related crops, forage grasses, bamboo and sugar cane.

Description

The method of control insect pest

The present invention relates to by using toxin protein, control Lepidoptera Ostrinia spp kind in crop plant, the method of Ostrinia furnacalis (Asiatic corn borer) preferably, toxin protein can obtain from bacillus thuringiensis (Bt) and/or other bacillus kind.

Bacillus thuringiensis belongs to big group of Gram-positive, for aerobic, forms endosporic bacterium.With other very near bacillus kind of affiliation, as Bacillus cercus or Bacillus anthracis difference, up to the present known bacillus thuringiensis bacterial classification major part all produces class gemma inclusion body when forming spore, because its crystal structure is commonly referred to as crystal.This crystal contains the former albumen of crystal toxin that tool kills insect active, i.e. delta-endotoxin.

Bacillus thuringiensis is because this albumin crystal to the toxicity of insect.Take in crystal at mouthpart, crystal is dissolved in before the intestinal juice of target insect, and delta-endotoxin does not show it and kills insect active.In most of the cases, cause proteolytic cleavage, cause discharging the effective toxicity composition from toxogen by the gastral followed by action of proteolytic enzymes of insect.

The delta-endotoxin of different bacillus thuringiensis bacterial strains is characterized in that to some target insect especially corresponding to different Lepidopteras, coleoptera and dipterous larva have high degree of specificity and at these susceptible larvas high activity arranged.The further advantage of bacillus thuringiensis delta-endotoxin is this toxin to the mankind, other mammal, and bird and fish are harmless.

According to their field of activity and sequence similarity, will kill the classification of insect crystalline protein from the difference of bacillus thuringiensis.Press Hofte and Whiteley, the classification that microbiology summary 53:242-255 (1989) proposes is divided into four big classes with the known insect crystalline protein that kills.Usually, class is divided by their field of activity greatly, and Cry I albumen is effective to Lepidoptera, and Cry II albumen is all effective to wing squama order and diptera, and Cry III albumen is effective to coleoptera, and Cry IV albumen is effective to diptera.In each big class, delta-endotoxin is divided into groups according to sequence similarity.

Cry I albumen is typically synthetic with 130-140KDa toxogen albumen, and kills the toxin protein of insect active through the tool that proteolytic cleavage produces about 60-70KDa size.The active part of delta-endotoxin is positioned at the aminoterminal part of full-length molecule.Hofte and Whiteley the samely are divided into six groups with known Cry I albumen, I A (a), I A (b), I A (c), I B, I C and I D.Since then, classification called after Cry I E, Cry I F, Cry I G, the albumen of Cry I H and Cry I X also obtain identifying.

The insect active scope of killing from each delta-endotoxin of bacillus thuringiensis is tending towards very narrow, and the delta-endotoxin of being given is only effective to some insects.Specificity be since produce the efficient of each step that active toxin albumen relates to and its subsequently with the interactional ability of insect gastrointestinal epithelial cells.

One of purpose of the present invention provides the method for control Ostrinia furnacalis (Asiatic corn borer) kind in plant, and described plant optimization crops include, but are not limited to corn, wheat, rye, oat, paddy rice, jowar, broomcorn millet and relevant crop, forage grass, kinds such as bamboo and sugarcane.This purpose can be within the scope of the invention, and is accomplished astoundingly by toxin protein from bacillus thuringiensis to the crop plant that will protect such as the Cry I type toxin protein of using.In another embodiment of the invention, toxin protein can obtain from the asexual culture of bacillus kind, and so-called asexual insecticidal protein (VIPs) is as VIP3[EP-A 0 690 916; International application no EP95/03826 quotes in full this open file herein as a reference], also can be used to control Ostrinia furnacalis (Asiatic corn borer) insect pest.

Therefore; the present invention relates to the method that protective plant and offspring thereof avoid the infringement that caused by Ostrinia furnacalis (Asiatic corn borer) kind; comprise to claimed plant or plant seed or plant growing zone and directly or indirectly use toxin protein pure or use the bacillus kind with the insecticidal mixtures form that contains a kind of described albumen or microorganism at least; preferably above-mentioned Cry I type or VIP type albumen, mentioned microorganism preferably contains the bacillus thuringiensis and/or the bacillus cereus strain of the toxin gene of at least one toxin-encoding albumen.The mentioned microorganism of Shi Yonging can be or naturally occurring bacterial strain in the method according to the invention, perhaps selectively, contains the recombinant bacterial strain of the recombination gene of toxin-encoding.

In preferred embodiments, use genetically modified plants to use the infringement that toxin is caused by Ostrinia furnacalis (Asiatic corn borer) with opposing for the plant that will protect.Such plant can obtain by the toxin gene that kills insect toxins albumen that transforms coding bacillus kind, above-mentioned insect toxins albumen extremely is such as the Cry type, preferably Cry I type toxin protein or VIP type albumen, and when planting such plant transformed in the area that above-mentioned insect pest occurs, genetically modified plants reach above-mentioned toxin protein so that the scale at the control of Ostrinia furnacalis (Asiatic corn borer) kind enough to be provided.

The protection crop plant of Shi Yonging is with the insecticidal mixtures of opposing Ostriniafurnacalis (Asiatic corn borer) insect pest in the method according to the invention; comprise at least a Cry type toxin protein for instance as active component; more preferably at least a Cry I type toxin protein; even preferred at least a Cry I A type toxin protein; especially preferably at least a Cry I A (b) type toxin protein; especially most preferably at least a Cry I A (b) type toxin protein according to SEQ ID NOs53-55; even more preferably contain the bacillus thuringiensis or the microorganism of the gene of at least one above-mentioned toxin protein of encoding; the bacillus thuringiensis bacterial strain that preferably contains the gene of at least one above-mentioned toxin protein of encoding; or derivatives thereof or mutant strain; in conjunction with adjuvant on the agronomy such as carrier; thinner, surfactant or use the promotion adjuvant.The active component that comprises in insecticidal mixtures also can be disclosed VIP type toxin protein or Cry I type and a VIP type protein combination in EP-A-0690916 and PCT international application no EP95/03826.VIPI type albumen preferably in the scope of protection; such as VIP1A (a) albumen or VIP1A (b) albumen; or VIP2 type albumen; such as VIP2A (a) albumen or VIP2A (b) albumen; or the combination of VIP1 type albumen and VIP2 type albumen; or VIP3 type albumen, such as VIP3A (a) albumen or VIP3A (b) albumen.

In protection domain more preferably at SEQ ID NOs:1,2,4-7,17-24,26-32, the VIP type toxin protein of expression in 35,36,39,40,42,43,45,46,49,50,51 or 52.

Composition also can comprise further bioactive compound.Above-claimed cpd can be that fertilizer or micronutrient donor or other influence the goods of plant growing.It also can be weed killer herbicide, the insecticide of selecting, fungicide, bactericide, the mixture of nematocide, invertebrate poison or several such goods, if desired, can be further with the conventional agronomy that uses in preparation technique on acceptable carrier, surfactant or use and promote the adjuvant combination.Appropriate carriers and adjuvant can be solid or liquid, and the general material that uses in the corresponding preparation technique, mineral matter, solvent, dispersant, wetting agent, sticker, adhesive or fertilizer for example natural or regeneration.

By weight, composition can comprise the active component of 0.1-99%, the solid of 1-99.9% or liquid adjuvant and 0-25% surfactant.Active component or contain above-mentioned composition of active components can with a certain other insecticide or chemicals (1993 crop protection chemicals reference manuals; chemicals and medicine publishing house, Canada) want protective plant or crops and do not lose effectiveness in conjunction with being administered to.It can spray substances compatible with other general agricultural of great majority, if but relate to Cry I type toxin, then should not be in the strong basicity spray solution and use.It can be with powder, suspension, wettable powder or anyly agricultural is used other suitable material form use.

Active component; i.e. preferably the Cry I type toxin protein of bacillus thuringiensis and/or aforesaid arbitrary VIP type albumen; or contain above-mentioned composition of active components and can be administered to the environment that (a) has insect pest; (b) part of plant or plant; avoid insect pest for a part of protecting this plant or plant and cause infringement; or (c) seed, avoid the infringement of insect pest in order to protect the plant that grows from this seed.

The method for optimizing of using in the plant protection zone is to use (foliar application) to the foliar spray of plant, and quantity of using and the frequency of using are according to the floristics that will protect and the risk factor that is subjected to these insect pest invasion and attack.

The composition of Shi Yonging also is suitable for the protective plant propagating materials in the method according to the invention, seed for example, and such as fruit, stem tuber or seed, or plant transplants a cutting, and avoids insect pest.Can handle propagating materials with preparation before plantation, for instance, seed can be mixed medicine prior to seeding.Active component of the present invention also can be by soaking seed or being administered to seed (coating) by covering seed with solid formulation with liquid formulations.Formulation also can be applied in planting location, the seed pears ditch when for example sowing when the plantation propagating materials.The present invention also relates to these methods of treatment of plant propagation material and the plant propagation material of handling like this.

Within the scope of the invention, composition can be used by any known method with bacterial isolates processing seed or soil.For example, see U.S. Patent number 4,863,866.Even bacterial strain is also effective to BIOLOGICAL CONTROL in the situation that microorganism does not survive.Yet preferably, use the microorganism of survival.

Claimed within the scope of the invention target crops are host plants of those Ostrinia furnacalis (Asiatic corn borer); include but not limited to corn, wheat, barley, rye, oat, paddy rice, jowar, broomcorn millet and relevant crops, forage grass, bamboo and sugarcane.

Can be used in combination with the form of unmodified or with acceptable carrier on any suitable agricultural according to active component of the present invention.Such carrier is the conventional adjuvant that uses in the agricultural formulations technology, and press known method preparation or emulsible concentrate thus, can cover paste, solution that directly can spray or soluble, dilution emulsion, wettable powder, soluble powder, medicinal powder, particle, and capsule, for example with polymer form.As the character of composition, application process as sprinkling, spraying, dusting, dispersion or pouring, also can be selected according to intended purposes and common situation.The ratio of effectively using from per hectare (" ha " about 2.471 acres) approximately 50g to changing about 5kg active component (a.i.), preferably from about 100g to about 2kg a.i./ha.The preferred ratio of using is that 200g arrives about 1kga.i./ha) or 200g-500ga.i./ha.

For seed dressing, the ratio of effectively using changes to the 1000ga.i. from every 100kg seed 0.5g, preferably from every 100kg seed 3g to 100ga.i..Most preferably use ratio from every 100kg seed 10g to 50g a.i..

Suitably carrier and adjuvant can be solid or liquid, and corresponding in preparation technique the general material that uses, mineral matter, solvent, dispersant, wetting agent, sticker, adhesive or the fertilizer of for example natural or regeneration.Formulation, it is insecticidal mixtures, goods or itself and other active component and suitable solid or the mixture of liquid adjuvant, can be by known method preparation, for example pass through active component and filler, solvent for example, the surface active cpd (surfactant) under solid carrier and some situation evenly mixes and/or grinds.

Suitable solvent is: aromatic hydrocarbon, the component that preferably contains 8-12 carbon atom, the naphthalene of xylene mixture or replacement for example, phthalic acid ester such as dibutyl phthalate or dioctyl phthalate, aliphatic hydrocarbon such as cyclohexane or alkane, pure and mild dihydroxylic alcohols and their ether and ester, as ethanol, 1,2-ethylidene glycol monomethyl or single ethyl ether, ketone such as cyclohexanone, intensive polar solvent such as N-N-methyl-2-2-pyrrolidone N-, the inferior maple of dimethyl or dimethyl formamide and vegetable oil or epoxidized vegetable oil such as epoxidation cocoa butter or soya-bean oil; Or water.

Used solid carrier for example for medicinal powder and can divide divided powder, generally is that the natural minerals filler is separated worm, talcum powder, kaolin, imvite or Attagel as the side.In order to improve physical characteristic, also may add the silicic acid of highly dispersion or the absorbent polymer of high degree of dispersion.Suitable particle absorption carrier is a porous type, float stone for example, brickbat soil, sepiolite or bentonite; Suitable non-absorption carrier is the material such as calcite or sand.A large amount of in addition tools material that is pre-formed particle inorganic or organic character also can use, for example, and especially dolomite or powdery plant residue.

According to the character of the active component that will prepare, suitable surface active cpd is that fine emulsification is arranged, nonionic, cation and/or the anion surfactant of dispersion and moistening character.Term " surfactant " also will be interpreted as and comprise surfactant mixture.Suitable anion surfactant can be water-soluble soap and water-soluble synthetic surface active cpd.Suitable soap is the alkali metal salt of higher fatty acid, alkali salt or ammonium salt (C unsubstituted or that replace ₁₀-C ₂₂), for example, oleic acid or stearic sodium salt or sylvite, or for example, the natural acid mixture that can obtain from cocoa butter or tallow oil.More suitable surfactant is fatty acid methyl taurate and modification and phosphatide unmodified also.

Yet, more frequently, use so-called synthetic surfactant, especially aliphatic sulfonate, aliphatic sulphate, sulphonated benzimidazole derivative or alkylaryl sulfonate.Aliphatic sulfonate or sulphate is usually with alkali metal salt, alkali salt or ammonium salts unsubstituted or that replace, and generally contain C ₈-C ₂₂Alkyl also comprises the alkyl component of acyl group, for example the sodium salt of lignin sulfonated acid and dodecyl sulphate or calcium salt; The mixture of the fatty alcohol sulphate that can obtain from natural acid maybe.These compounds comprise the sulphate and the sulfonate of fatty alcohol/ethylene oxide adduct.Sulphonated benzimidazole derivative preferably contains 2 sulfonic acid groups and a fatty acid group that contains about 8-22 carbon atom.The example of alkylaryl sulfonate is a DBSA, the sodium salt of dibutyl naphthalene sulfonic acids or naphthalene sulfonic acids/formaldehyde condensation products, calcium salt or triethanolamine salt.Corresponding phosphate also is fit to the phosphate ester salt of the adduct of for example right-nonyl phenol and 4-14 moles of ethylene oxide.Non-ionic surface active agent is the polyglycol ether derivative of aliphatic alcohol or cycloaliphatic alcohol preferably, or saturated or unsaturated fatty acid and alkyl phenol, said derivative contains in 3-30 ethylene glycol ether group and 8-20 the carbon atom in the aliphatic hydrocarbon component and contain 6-18 carbon atom in the alkyl component of alkyl phenol.

More suitable non-ionic surface active agent is PEO and the polypropylene glycol that contains 1-10 carbon atom in alkyl chain, the water-soluble adduct of ethylenediamine polypropylene glycol and alkyl polypropylene glycol, this adduct contain 20-250 ethylene glycol ether group and 10-100 propylene glycol group.These each propane diols units of compound contain a 1-5 ethylene glycol unit usually.The representational example of non-ionic surface active agent is a nonyl phenyl polyethoxy ethanol, castor oil polyglycol ether, poly(propylene oxide)/ethylene oxide adduct, tributyl phenoxy group polyethoxy ethanol, polyethylene glycol and decyl phenoxy group polyethoxy ethanol.The fatty acid ester of polyethylene glycol oxide sorbitan as polyethylene glycol oxide sorbitan trioleate, also is suitable nonionic detergent.

Cationic detergent is quaternary amine preferably, comprises the substituting group as N-, at least one C ₈-C ₂₂Alkyl, and further substituting group, alkyl, benzyl or hydroxy lower alkyl rudimentary non-replacement or halo.This salt is preferably with halide, the form of dimethyl suflfate or dithyl sulfate, for example chlorination stearoyl Trimethylamine or bromination benzyl two-(2-chloroethyl) ethyl ammonium.

The surfactant that custom is used in preparation technique is for example being described in the following document: " detergent of McCutcheon and emulsifier yearbook " MC publishing company.Ridgewood, N.J., 1979; Dr.Helmut Stache, " Tensid Taschenbuch " (surfactant handbook), Carl Hanser Verlag, Manich/vienna.

The persistence that another particularly preferred feature of insecticidal mixtures of the present invention is an active component when being applied to plant and soil.The possible cause that causes loss of activity comprises owing to ultraviolet light heat, the inactivation of leaf secretion and PH.For example, at high PH, when especially having reductant, the delta-endotoxin dissolution of crystals, thus become easier proteolysis inactivation.The high pH value of leaf also may be important, particularly the place in leaf surface PH can the scope at 8-10.The prescription of the insecticidal mixtures of Shi Yonging can help prevent the forfeiture of active component or material is wrapped in the capsule by adding additive in the method according to the invention, avoids inactivation with the method protection active component and solves these problems.Encapsulatedly can use chemical method McGuire and Shasha, J Econ Entomol85:1425-1433,1992) or biological method (Barnes and Cunmings, 1986; EP-A0192319) carry out.Chemiluminescent capsuleization relates to the step with polymer coating active composition, and biological capsuleization relates to expressing delta-endotoxin genes in microorganism, for biological capsuleization, uses to comprise the complete microorganism of toxin protein as active component in prescription.Add ultra-violet protecting agent and can reduce radiation damage effectively.Because the inactivation that heat causes also can be by adding suitable additive control.

If possible, preferably comprise the prescription of the microorganism of survival as active component in the present invention, mentioned microorganism or with the form of asexual cell is perhaps more preferably with the form of spore.Proper formula can comprise, and for example with the crosslinked polymer latex of multivalent anions, and comprises these microorganisms.For example by D.R.Frarel etc. in plant pathology, Vol.75, No.7,774-777 describes alginates as polymeric material in 1985.From then on openly know that also carrier material can be used together in the text.These prescriptions can prepare in the following manner: by with natural existence or synthetic gel forming polymer, for example alginates and polyvalent metal ion saline solution mix, forming single drop, thereby, might be suspended in one of two reaction solutions or in two reaction solutions for microorganism.The formation of glue is from mixing with the drop form.May be the drying of these glue particles subsequently.This process is called ion gelatification.According to degree of drying, formed tight and hard polymer beads, it is structurally crosslinked by polyvalent cation, and comprises general equally distributed microorganism and carrier.Particle size can reach 5mm.

Described in EP-A1-0097571 based on partial cross-linked polysaccharide and the composition of microorganism, it for example also can comprise the meticulous silicic acid that separates as carrier material; For example pass through Ca ⁺⁺Ion takes place crosslinked.Said composition has and is no more than 0.3 water activity.W.J.Cornick etc. are in the survey article [new trend in the BIOLOGICAL CONTROL: suppress agricultural and go up improving one's methods of insect pest and disease, the 345-372 page or leaf, Alan R.Liss, Inc. (1990)] in different prescription systems has been described, with vermiculite as the particle of carrier and the alginate beads closely for preparing by described ion gelatification method.Composition such in killing insect prescription and application system is also open by D.R.Fravel: 11 volumes, and test of the ASTM STP1112 U.S. and materialogy meeting, Philadelphia, 1992, the 173-179 page or leaf, and can be used for preparation according to recombinant microorganism of the present invention.For the further method of preparation survival microorganism is described in WO96/02638.

Even when using ratio, also preventative the and/or therapeutic treatment to insect pest control field is useful low for composition according to the present invention, it is for the homoiothermy kind simultaneously, fish and plant be tolerance with avirulent, and have very suitable biocide scope.Can resist all of Ostrinia furnacalis (Asiatic corn borer) insect pest or independent developmental stage effectively according to composition of the present invention.The insect that kills according to The compounds of this invention acts on and can directly display, and promptly eliminating pest immediately perhaps only manifests after having spent a period of time.

Above-mentioned composition can provide with the form of the chemical mixture that comprises pure basically toxin protein or with the form of mixtures of the toxin protein that comprises an at least a part as microorganism or genetically modified plants.

In the specific embodiment of the present invention, one of active component can directly pass through, for example aforesaid foliage spray is administered to plant as this paper, and another active principle can be provided by the gene of coding above-mentioned another principle of plant by expressing prior conversion itself.

The insecticidal mixtures of Shi Yonging contains usually from about 0.1% to about 99% in the method according to the invention, preferably from about 0.1 to about 95%, most preferably from about active component of 3 to 90%; From about 1 to about 99.9%, preferably from about 1 to about 99%, most preferably from about 5 to about 95% solid or liquid adjuvant; With from about 0 to about 25%, preferably about 0.1 to about 25%, from about 0.1 to about 20% surfactant most preferably.

Commercial product preferably is mixed with concentrate simultaneously, and the end user generally will use the in fact dilution preparation liquid of lower concentration, insecticidal mixtures also may comprise further composition, such as stabilizing agent, defoamer, viscosity modifier, adhesive, sticker, fertilizer or other active component are to obtain special effect.

The present invention also relates to comprise the survival microorganism is the prescription of active component, and if possible, mentioned microorganism is with asexual cell or exist with spore form more.

Further purpose of the present invention relates to the application of avoiding recombinant microorganism in the method for the infringement that caused by Ostriniafurnacalis (Asiatic corn borer) kind at the control crop plant.This recombinant microorganism contains the toxin gene of coding bacillus thuringiensis toxin protein such as Cry I type albumen; this recombinant microorganism or directly be administered to the plant that will protect, the toxin protein that perhaps is re-combined into separate and as above-mentioned preparation from recombinant microorganism earlier before being administered to the crop plant that will protect.Recombinant microorganism also can comprise the toxin gene that is coded in disclosed VIP type toxin protein among EP-A-690916 and the international application no NOEP95/03826 or the combination of the gene of encode respectively at least a Cry type toxin and VIP type toxin.

In order to be re-combined into toxin protein in host living beings, coded sequence can be inserted in the Expression element of selected host's design and import the host, is re-combined in the host.Selection is suitable for selected host's specific regulating sequence such as promotor, burst, and 5 ' and 3 ' non-translated sequence and enhancer are within practitioner in the art's technical merit.The molecule of gained has comprised the particular element that connects in the suitable reading frame, is inserted in the carrier that can be transformed in the host cell.Well-known for the expression vector that various host living beings are fit to the method that is re-combined into albumen.Above-mentioned host living beings comprises as Escherichia coli [sees Studier and Moffatt for example, molecular biology magazine 189:113 (1986); Brosius, DNA 8:759 (1989)], and yeast [seeing Schneiderand Guarente for example, Enzymology method 194:373 (1991)] and insect cell [see, for example, Luckow and Summers, biology/biotechnology 6:47 (1988)].Special example comprises plasmid such as pBluescript (Stratagene, La Jolla, CA), pFLAG (International Biotechnologies, Inc., New Haven, CT), pTrcHis (Invitrogen, La Jolla, CA) and rhabdovirus expression vector, those carriers that derive from from Autographa californica multicapsid nucleopolyhedrosisvirus nuclear polyhedrosis virus genome for example.Preferred baculoviral/insect system be the PVI11392/SF21 cell (Invitrogen, La Jolla, CA).

The toxin protein that is re-combined into can use the series of standards technical point from and purifying.Operable effective technology will be according to used host living beings, whether toxin protein is designed to such factor change that secretion and other those skilled in the art recognize [is seen, for example Ausubel, F. etc. " molecular biology routine techniques " is by John Wiley﹠amp; Sons company publishes, (1994) the 16th chapters].

The preferred purpose of the present invention relates to the application of avoiding genetically modified plants in the method for the infringement that caused by Ostrinia furnacalis (Asiatic corn borer) insect pest at the protection crop plant; these genetically modified plants comprise and to be enough to provide the toxin protein that reaches the coding bacillus thuringiensis at the scale of the control of Ostrinia furnacalis (Asiatic corn borer) kind, the toxin gene of Cry I type toxin protein especially.Plant can be result's (seeing WO95/24492) that consideration conveyization or plasmid transform.Particularly preferably be the genetically modified plants of Cry I A (6) toxin protein of expressing bacillus thuringiensis.The application of the genetically modified plants of the toxin gene of the VIP type albumen that the present invention also relates to comprise coding as in EP-A-690916 and international application no EP95/03826 (be incorporated by reference in this text herein and examine), describe.The present invention also relates to comprise and express the toxin protein of coding bacillus thuringiensis, but the application especially toxin gene of Cry type toxin protein and that also comprise and enough provide the genetically modified plants of the virulent gene that scale at the control of Ostriniafurnacalis (Asiatic corn borer) kind reaches coding VIP type albumen.The host plant of expressing above-mentioned toxin gene will have enhancing to the resistance of Ostrinia furnacalis (Asiatic corn borer) kind insect infestations and will reduce the crop loss relevant better with this invasion and attack.

In a preferred embodiment, in genetically modified plants, express one or more Bt delta-endotoxins and follow one or more VIP type albumen of expression.Coexpression can be by the genetic manipulation plant to comprise and to express all and must realize by gene more than a kind of insect principle of killing in same genetically modified plants.Selectively, a plant species, the parent 1, can be through genetic engineering procedure to express VIP type albumen.Another plant, the parent 2, can be through genetic engineering procedure to express the Bt delta-endotoxin.By parent 1 and parent's 2 hybridization, all genes that import parent 1 and parent 2 are expressed in the filial generation that obtains.Particularly preferred Bt delta-endotoxin is those disclosed in EP-A0618976, quotes as a reference herein.

The present invention includes contain coding can be under the moderate stringent condition and the dna molecular of coding bacillus kind toxin protein, but preferably with the recombinant microorganism of the gene of the dna molecular of the oligonucleotide probe hybridization that can obtain from above-mentioned dna molecular or the application of genetically modified plants, wherein above-mentioned probe grows to the continuous part that few 10 nucleotide comprise the coded sequence of above-mentioned toxin protein.The present invention preferably includes the dna molecular of the toxic protein of coding and coding bacillus thuringiensis or Bacillus cercus, especially with the dna molecular or the coding VIP type toxin protein of coding Cry type albumen, the preferably recombinant microorganism of the gene of the dna molecular of the toxin gene of Cry I A (b) albumen hybridization or the application of genetically modified plants.

Influence the strict degree of the factor decision hybridization of hybrid stability.One of such factor is melting temperature Tm, and Tm can be easily obtain calculating according to formula, and formula is at dna probe, GeorgeH.Keller and Mark M.Manak, Macmillan Publisher Ltd, 1993, first: molecular hybridization; Provide in the 8th page.

Preferred hybridization temperature calculates in the about 25 ℃ scope of melting temperature Tm being lower than, and preferably in being lower than the scope of calculating about 12-15 ℃ of melting temperature Tm, and is being lower than under the situation of oligonucleotides in the about 5-10 of melting temperature Tm ℃ the scope.

The invention further relates to and be used for insect pest at crop plant control Ostrinia furnacalis (Asiatic corn borer), the commodity bundle that comprises transgenic plant seed and reliable operation instruction, above-mentioned genetically modified plants comprise at least one toxin gene, and to be enough to provide scale to reach this toxin gene at the control of Ostriniafurnacalis (Asiatic corn borer) kind, the toxin protein of this toxin gene coding bacillus thuringiensis, Cry type toxin protein preferably, Cry I type toxin protein more preferably, but Cry I A type toxin protein most preferably.In the present invention, preferably comprise the gene that comprises coding at least one Cry type toxin protein or VIP type toxin protein commodity bundle as the transgenic plant seed of active component.It particularly preferably is the combination of Cry I A (b) toxin protein and VIP type albumen.

Further aim of the present invention is to be used for the insect pest at crop plant control Ostrinia furnacalis (Asiatic corn borer), comprises the commodity bundle according to insecticidal mixtures of the present invention and reliable operation instruction thereof.

For plant, be meant any floristics that can carry out genetic transformation by methods known in the art, but those plants of the host plant of Ostrinia furnacalis (Asiatic corn borer) kind especially, include, but are not limited to following floristics: corn, wheat, barley, rye, oat, paddy rice, jowar, broomcorn millet and relevant crop, forage grass, bamboo (orchard grass, fescue grass, or the like), and sugarcane.

Method in Plant Transformation known in the art is discussed below.Host plant as the purpose crop includes, but are not limited to those kinds listed above.

The invention further relates to the gene of the toxin protein that comprises the bacillus thuringiensis of encoding and enough providing scale to reach the seed of the genetically modified plants of this toxin protein at the control of Ostrinia furnacalis (Asiatic corn borer) kind, and the commodity bundle that comprises this seed.

For plant, be meant any floristics of Ostrina furnacalis (Asiatic corn borer) host, include, but are not limited to corn, wheat, barley, rye, oat, paddy rice, jowar, broomcorn millet and relevant crop, forage grass, bamboo and sugarcane.

The password usage of having found natural bacillus thuringiensis toxin gene is obviously different with the typical usage of plant gene.Especially, the codon usage of natural bacillus thuringiensis gene and corn gene is very different.Therefore, this gene mRNA may not effectively utilize.Codon usage may the translation transcribe or the mNRA level of processing on influence expression of gene.For making toxin protein in plant, optimum expression in corn for example, most preferred codon makes the codon usage optimization in corn by using in the synthetic gene of the identical albumen of composite coding and natural toxin gene order coding.Optimized preferred corn codon usage is effective to high level expression Bt insecticidal protein.The further details that makes up the optimized synthetic toxin gene of corn can obtain in WO/3/07278, is incorporated by reference in this text herein and examines.

Toxin gene from microorganism also may be different with plant gene.The gene difference of finding in plant gene and the microorganism is that the RNA that they are transcribed does not produce the specific ribosome bind site sequence of contiguous initial methionine.So microbial gene can add strongly expressed by insert the conservative translation initiation district of eucaryote at the ATG place.Clontech (1993/1994 catalogue, 210 pages) proposed sequence GTCGACCATGGTC is as the translation initiation district of expressing Escherichia coli uidA gene conservative in plant.In addition, Joshi[nucleic acids research 15:6643-6653 (1987)] compare the plant sequence of some contiguous ATG and proved conserved region TAAACAATGGCT.Under the situation that expression microorganism ORFs meets difficulty in plant, insert such sequence at initial ATG place and may improve translation.Because the modification of second amino acid residue is not suitable for back three nucleotide of conserved region are included in the modification sequence in this case.The sequence of preferred contiguous initial methionine may be different between different floristics.By the sequence of the corn gene that in the GenBank/EMBL database, provides, can find that the nucleotide of which contiguous ATG should be modified the translation that is transformed into the toxin gene in the corn with enhancing.

In addition, shown that the unreasonable shearing site of removal can strengthen the expression and the stability of quiding gene.From non-plant source clone, the gene that is unsuitable for expressing in plant may comprise some patterns, these patterns can be identified as 5 in plant ' or 3 ' shearing site.Thereby transcription may be ended in prematurity, produces mRNA brachymemma or disappearance.Toxin gene can be processed to remove these unreasonable shearing sites.

The delta-endotoxin proteins of some bacillus thuringiensiss is expressed as toxogen.These toxogens dissolve in the alkaline environment of insect stomach and cut into poisonous core fragment [Hofte and whiteley, microorganism summary, 53:242-245 (1989)] by proteolytic enzyme.For the delta-endotoxin proteins of Cry I class, poisonous core fragment is positioned at the N end parts of toxogen.The gene of the poisonous core fragment of coding that uses in the plant conversion carrier that brings the host plant insecticidal properties or total length toxogen form or complete toxin protein brachymemma all within the scope of the present invention.

Recombinant DNA molecules can import plant cell with a lot of art-recognized methods.Those skilled in the art will agree that the selection of method may depend on the target vegetation type that will transform, i.e. unifacial leaf or dicotyledonous.The proper method of transformed plant cells comprises microinjection [Crossway etc., biotechnology 4:320-334 (1986)], electroporation [Riggs etc., institute of NAS reports 83:5602-5606 (1986)], the conversion [Hinchee etc., biotechnology 6:915-921 (1988)] of soil Agrobacterium mediation, direct gene transforms [Paszkowski etc., EMBO is (1984) J.3:2717-2722], quicken with the bullet grain, use from Agracetus Inc., Madison, Wisconsin and Dupont, Inc., Wilmington, the available instrument of Delaware [is seen, for example, Sanford etc., United States Patent (USP) 4,945,050; With McCabe etc., biotechnology 6:923-926 (1988)].Also see Weissinger etc., genetics yearbook 22:421-477 (1988); Sanford etc., particulate science and technology 5:27-379 (1987) (ion); Christou etc., plant physiology 87:671-674 (1988) (soybean); McCabe etc., biology/biotechnology 6:923-926 (1988) (soybean); Datta etc., biology/biotechnology 8:736-740 (1990) (paddy rice); Klein etc., institute of NAS newspaper, 85:4305-4309 (1988) (corn); Klein etc., biology/biotechnology 6:559-563 (1988) (corn); Klein etc., plant physiology 91:440-444 (1988) (corn); Fromm etc., biology/biotechnology 8:833-839 (1990); With Gordon-Kamm etc., plant cell 2:603-618 (1990) (corn); Svab etc., institute of NAS report 87:8526-8530 (1990) (tobacco protoplast); Koziel etc., biotechnology 11:194-200 (1993) is (maize); Shimamoto etc., natural 338:274-277 (1989) (paddy rice); Christou etc., biotechnology 9:957-962 (1991) (paddy rice); European patent application EP 0332581 (orchard grass and other Pooideae); Vasil etc., biotechnology 11:1553-1558 (1993) (wheat); Weeks etc., plant physiology 102:1077-1084 (1993) (wheat); Wan etc., plant physiology 104:37-48 (1994) (barley); Umbeck etc., biology/biotechnology 5:263-266 (1987) (cotton).

Particularly preferredly a series of embodiments that recombinant DNA molecules imports corn can be found in WO93/07278, be incorporated by reference in this text herein and examine by microparticle bombardment.Another embodiment preferred is the protoplast transformation method of disclosed corn in application EP-A-292435, is incorporated by reference in this text herein and examines.

Breeding of genetic characteristics trafficability characteristic or the asexual growth of above-mentioned importing transgenic seed and plant are gone down to posterity, and therefore also can keep in progeny plant and breed.Usually above-mentioned keep and breed to use become known for adapting to specific purpose as ploughing sowing, or the agricultural methods of results.Also can use training of special method such as water ballast or greenhouse technology.Because the crops that growing are easy to be subjected to the competition of insect or the invasion and attack of infecting and infringement and ruderal plant, take to control weeds, plant disease, insect, the way of nematode and other bad condition is to improve output.The plant that these comprise mechanical means such as mellow tilth or remove weeds and infect, and use agricultural chemicals such as weed killer herbicide, fungicide, gametocide, nematocide, growth regulator is urged thermit powder and insecticide.

Useful hereditary capacity according to genetically modified plants of the present invention and seed can further be used in plant breeding, and the genetic breeding purpose is to develop the plant of tool improved characteristics, as to insect pest, the tolerance of weed killer herbicide or adverse circumstance, improve nutritive value, increase output, or improve the structure decrease lodging.Various breeding steps are feature with the human intervention of good management, as the strain system of selecting to hybridize, and the pollination of mediation parent plant system, or select suitable progeny plant.According to required feature, adopt different breeding methods.Correlation technique includes but not limited to hybridization, inbreeding, back cross breeding, polyphyly breeding, multiple mixing, interspecific cross, aneuploid technology etc. known in the art.Hybridization technique also comprises by machinery, and chemistry or biochemical method make plant sterile to produce male or plants with female sterility.Male sterile plants and the pollen crossing pollination of the system of homophyletic have not been guaranteed the genome of male sterile plants, and female fertile plants obtains equalization the feature of two parent plants systems.Like this, can be used to cultivate the plant strain system of improvement according to transgenic seed of the present invention and plant, the efficient of conventional method such as weed killer herbicide or pesticide treatments for example improves in this strain system, or since the hereditary feature of their improvement promote with said method.Selectively, can obtain the new crop of the improved stress resistance of tool, since their optimized heredity " element ", the results product that output is more high-quality than the product that can not tolerate corresponding bad developmental condition.

During seed was produced, the germination quality and the homogeneity of seed were necessary product performances, and the germination quality and the homogeneity of the seed of being gathered in the crops and selling by the peasant are unimportant.Because be difficult to the influence that the control crop is not subjected to other crops and weed seed, in order to control kind of living disease and to produce good seeds germinated, seed producers has developed the seed production method of quite extensive and fine management, and seed producers has experience in growth in regulation and control and the purebred field of market sale.Common method is that the peasant buys the seed that has detected that satisfies the certain quality standard, rather than uses from the seed of the crop harvesting of oneself; The propagating materials such as the seed that use are handled with protective coating routinely, and protective coating comprises weed killer herbicide, insecticide, fungicide, bactericide, nematocide, gametocide or their mixture.The conventional protective coating that uses comprises such as captan, the spirit of green bristlegrass rust, tmtd (TMTD ^_), methalaxyl (Apron ^_) and; Actellic (Actellic ^_) compound.If desired, these compounds further with other conventional carrier that uses in preparation technique, surfactant or use and promote adjuvant that the infringement to provide protection opposing bacterium, fungi or animal pests to cause is provided together.Protective coating can be by using with liquid formulation dipping propagating materials or by the wet or dried formulation coating with combination.Other application process also is possible, as directly handling on bud or fruit.

Another aspect of the present invention provides new agricultural methods, as the above method of enumerating, the method is characterised in that use according to genetically modified plants of the present invention, transgenic plant material, or transgenic seed is to provide the control at Ostrinia furnacalis (Asiatic corn borer).

For breeding filial generation, can use: as known in the art, grow in the basin in greenhouse or in soil as the corn plant of describing in the following embodiments that obtains, and make it to bloom as following method from the method according to this invention plants transformed.Obtain pollen from ripe male flower fringe, and be used for, the female flower fringe pollination of plant born of the same parents or any selected milpa to same plant.In the transformant that according to said method obtains generation, can and/or be followed the existence of DNA (genotype) by quiding gene, or the phenotype of bringing and unconverted filial generation differentiate.Transformant generation similarly selfing or with other plant hybridization, general and any plant hybridization that has required quality.Similarly, tobacco or other according to said method obtain transforming plant and can have the filial generation of desirable characteristics with generation as in selfing known in the art or hybridization.Similarly, by other genetically modified plants of producing in conjunction with methods known in the art and the present invention can as in cultivation known in the art to produce the filial generation of the required feature of tool.

Embodiment

Following examples have further described to finishing material and the method that particular of the present invention is used.They provide by the method for explanation, and should not be construed as and limit disclosing of this specification.Embodiment 1: conventional method

Dma operation uses the method for the conventional application in this area to carry out.These methods can modify under the situation that does not change the result basically and/or change.Except other document that has illustrated, certain methods is at books commonly used such as Sambrook etc., molecular cloning: laboratory manual, and publishing house of cold spring harbor laboratory, second edition is described in 1989.Embodiment 2: plant conversion carrier

Plant Transformation uses the conversion carrier PCIB4431 and the PCIB3064 that describe in (1993) [biotechnology Vol11194-200] such as WO93/07278 and Koziel to carry out, and two open source literatures are quoted as a reference herein.

PCIB4431 is the carrier of design with maize transformation.It comprises two chimeric synthetic Bt Cry I A (b) endotoxin genes that can express at corn, and one of them has made up PEP carboxylase promotor/synthetic Cry I A (b) gene, and another is pollen promotor/synthetic Cry I A (b) gene.

Synthetic Cry I A (b) gene that provides in SEQ ID NO:1 has been provided PCIB4431, and be stored in agricultural research institute preservation center (NRRL) on September 21st, 1992, northern regional study center, 1815 North University Street, Peoria, Illinois61604, U.SA, preserving number are NRRL B-18998.

PCIB3064 comprises the effable bar gene of plant (615bP), originally from streptomyces hygroscopicus clone [Thompson etc. (1987) EMBO J 6,2519-2523].Its phosphorus silk rhzomorph acyltransferase (PAT) of having encoded; bring resistance to phosphorus silk rhzomorph; the bar gene under the control of CaMV 35S promoter and terminator [institute of OW etc. (1987) NAS newspaper 84,4870-4874] so that the resistance to phosphorus silk rhzomorph to be provided.Embodiment 3 contains the generation of the transgenic corn plant of synthetic corn C ry I A (b) gene.

Following examples utilize the Biolistic instrument that the particle that DNA wraps quilt is imported maize cell, therefrom produce plants transformed.3.1 tissue

The prematurity maize, approximately long 1.5-2.5mm, after pollination 14-15 days, cut out from the female fringe of genotype 6N6 15, maternal plant grows in the greenhouse.Before excision, female fringe is with 20%Cloxox surface sterilization 20 minutes, and with aseptic water washing three times.Single embryonic shield sheet is placed in 2 square centimeters of zones up, 36 embryos of every dish, and place the callus of induce medium, the 2DG4+5 Amiben is cultivated [a large amount of salt of N6, the B5 trace salt, MS iron ion, 2% sucrose and 5mg/l Amiben, 20mg/l glucose, and adding 10mlG4 additive (table 1) behind the autoclaving] on.Table 1:G4 additive

Every liter of medium of composition

Casein hydrolysate 0.5g

Proline 1.38g

Niacin 0.2mg

Pyridoxine-HCl 0.2mg

Thiamine-HCl 0.5mg

Choline-HCl 0.1mg

Vitamin b3 0.05mg

Vitamin h 0.1mg

Folic acid 0.05mg

Calcium pantothenate 0.1mg

P-aminobenzoic acid 0.05mg

The preparation of the DNA that B12 0.136 μ g3.2 is used to transform

Microcarrier is according to the specification preparation that provides with the Biolistic instrument, in the time of earthquake 50 μ l1.0 μ m gold microcarrier, add 5 μ l pCIB4431 (1.23 μ g/ μ l) [#898]+2 μ l PCIB3064 (0.895mg/ μ l) [#456], add 50 μ l 2.5MCaCl again ₂, add 20 μ l0.1M spermidines (free radical, TC level) then.The mixture concussion mixing that obtains 3 minutes and centrifugal 10 seconds.Remove supernatant, and with 250 μ l, 100% ethanol (HPLC level) by of short duration concussion, centrifugal and removal supernatant washes microcarrier 2 times, microcarrier is resuspended in 65 μ l, 100% ethanol.3.3 bombardment

Use PDS-1000He Biolistic instrument bombardment tissue.Tissue is positioned on the grid of the following 8cm of braking screening baffle plate (stopping screen shelf), organizes once with the shooting of 10 μ l DNA/ gold microcarrier solution, and goes up dry at larger vector (macrocarrier).Used termination sieve plate uses the manual punching press of 10 * 10 stainless steel sieve meshes, use the 1550psi value from sliver.After the bombardment, embryo in the dark, 25 ℃ of cultivations.3.4 callus forms

Bombard after 1 day, embryo is transferred on the callus of induce medium that contains 3mg/l PPT.The callus of bombardment back 2 and 3 week assessment embryo forms.All embryos that respond are transferred to callus and are kept medium, contain the 2DG4+0.5 2 of 3mg/L PPT, on the 4-D medium.It is a large amount of salt of N6 that callus is kept medium, the B5 trace salt, and MS iron, 2% sucrose adds 0.5mg/L 2,4-D, 20mg/l glucose, and behind autoclaving, add 10ml G4 additive.Embryo is given birth to callus and is gone down to posterity with the fresh medium of keeping that contains 3mg/L PPT every 2 weeks, and all callus are in the dark in 25 ℃ of incubations.

It is 15% that I type callus forms reaction.Each produces the embryo of callus as situation cultivation separately, to form independent strain is.3.5 regeneration

After screening for 12 weeks, tissue is kept medium from the callus that contains PPT move on on the regeneration culture medium.Regeneration culture medium is 0.25MS3S5BA (0.25mg/L 2,4-D, 5mg/L BAP, MS salt, 3% sucrose), after 2 weeks, cultivates with regeneration plant on the MS3S medium again.After 5 to 10 weeks, take out plant, and put into GA7 ' S.Embodiment 4: analysis 4.1 elisa assay of transgenic corn plant

Cry I A (b) expression of gene detect to be used Asiatic corn borer insect bioanalysis and elisa assay monitoring in transgenic corns, with the level of Cry I A (b) albumen determining in quantity to obtain.

Use Enzyme Linked Immunoadsorbent Assay (ELISA) that Cry I A (b) insecticidal protein in the genetically modified plants leaf is carried out quantitative mensuration, as at Clark MF, ListerRM, Bar-Joseph M:ELISA technology.In: Weissbach A, Weissbach H (editor) Enzymology method 118:742-766, the academic press, Florida (1986) is open.Use the special polyclone of insecticidal protein to bacillus thuringiensis Ku Er Stark subspecies of immune affinity purifying to exempt from the amount from insecticidal protein in the soluble protein of sample leaf crude extract with the every mg of goat-anti body measurement.When the well of each ELISA microtiter plate used 50 μ g total proteins, the sensitivity of twin-stage sandwich ELISA was every mg soluble protein 1-5ng insecticidal protein.

Corn extract obtains by using hand-held ball homogenizer (AGDIA, ELkart IN.) to grind leaf texture in the plastic sack of netted line, and extraction buffer (50mMNa is arranged in the homogenizer ₂CO ₃PH9.5,100mM NaCl, 0.05%Triton, 0.05%Tween, 1mM PMSF and 1 μ M Leupeptim).Protein determination uses Bio-Rad, and (Richmond, CA) the albumen test is carried out.4.2 Asiatic corn borer is analyzed

Cut next from the leaf that milpa is launched to 4 4cm fritters.Each leaf piece is placed on the round filter paper moistening in 50 * 9mm culture dish.On each leaf piece, put 5 newborn Asiatic corn borer larvas (making a total 5-20 larva on each plant).Culture dish is placed on 29.5 ℃ of incubations.Assessment leaf piece is raised damage and lethality after 24,48 and 72 hours.Embodiment 5:Ostrinia furnacalis (Asiatic corn borer) field test experiments

To include the little mud basin that there is and has expressed the transgenosis seedling of transformed gene in prior test, transfer is in the field.Not genetically modified inbred line strain ties up to same field and carries kind and to surpass for six weeks, with in contrast and for pollination.

When the field plant reaches expansion length of blade 40cm, begin Ostrinia furnacalis (Asiatic corn borer) larva invasion and attack transgenosis and non-transgenic check plant with laboratory rearing.Use the Davis incubator, about 300 newborn larvaes that mix with the corncob grit are imported in the verticillate leaves of every strain plant.With the week is unit, and invasion and attack continue all around to stimulate first generation Asiatic corn borer.The two weeks beginning of initial invasion and attack back uses mark 1-9 to assess every strain plant weekly, and (1=does not have visible leaf damage around continuing; The most of leaves of 9=have long wound, and several leaves have the middle arteries and veins that fractures, and possibility is owing to feed the dwarfed plant that Asiatic corn borer causes).For every strain transfer-gen plant and non-transgenic adjoining tree, the average assessment mark of Asiatic corn borer damage obtains calculating.When each plant reaches flowering stage, use 300 larvas/plant weekly, around continuing, carry out the invasion and attack second time.Each 100 newborn larvaes in the corncob grit are imported the above of the axil of nascent female fringes and nascent female fringe knot and with the axil of next joint.Thus, about 2400 larvas of sum are added on each strain plant.All transfers and stems some non-transgenic plant are collected in about 50 days of initial secondary invasion and attack back.Identify the degree of the inner perforated damage in whole plants of invasion and attack for the second time.Embodiment 6: test is from the activity of the extract opposing Ostrinia furnacalis (Asiatic corn borer) that transforms protoplast

Use from moment transforming DNA with the extract that the maize cell of expressing corn optimization gene obtains, carry out the Western engram analysis.

Use the protoplasm of results to carry out quantitative insect toxicity test.Be each repeated experiments supending of in bioanalysis, being surveyed.If a repeated test causes the higher lethality of obvious comparison photograph, think that then it is positive.For example, use Asiatic corn borer, Ostrinia furnacalis retest extract is at the activity of lepidopterous insects.(Bioserv, Inc., frenchtown, NJ in the artificial black root eating insect nutrition of the protoplast hanging drop of 100 μ l in 0.1%Triton X-100 in the culture dish that snap-on covers is arranged at 50mm * 10mm; F9240).After air-dry, every culture dish adds 10 newborn larvaes.Write down lethality after about 4 days.Plant soak test 7.1 bacillus thuringiensiss (Bt) crystal of embodiment 7:Ostrinia furnacalis (Asiatic corn borer)

Bacillus thuringiensis (Bt) crystal is prepared into the 22ml distilled water stocks suspension.Suspension is stored in the refrigerator.7.2 record standard

Use 3 the biggest Ostrinia furnacalis (Asiatic corn borer) larvas on maize leaves, to raise.Larva is fed with untreated leaf in advance.After 120 hours, write down the quantity of dead larva.Observe each test middle period and go up the situation of raising damage.7.3 method of testing

Use is to two inbred line plant of isozygotying (strain is A and B) of the corn (Zea mays) of Asiatic corn borer susceptible.The biggest seedling of 9-10 is soaked in the Bt of variable concentrations protein suspending liquid, and uses in feeding experiment, wherein larva be placed on doing of seedling leaf on, every plant 5-10 larva.Seedling covers with nylon net bag, and is placed in the nylon wire cage.4 repeated experiments of 4-5 concentration of test are identified lethality.Temperature remains on 21-30 ℃.7.4 result

Table 2: strain is the result of A

Dosage (ppm)	The insect sum	The dead insects number	Lethality (%)
				?80	????40	????31	?77.5
?40	????40	????29	?72.5
				?20	????40	????15	?37.5
?10	????40	????13	?32.5
				??5	????40	????5	?12.5
??0	????40	????0	?0.00

Table 3: strain is the result of B

Dosage (ppm)	The insect sum	The dead insects number	Lethality (%)
				?80	????40	????38	?94.74
?40	????40	????33	?81.58
				?20	????40	????28	?68.42
?10	????40	????19	?44.74
				??5	????40	????10	?21.05
??0	????40	????2	?5.00

Two kinds of situations of maize leaves damage clearly distinguish: the leaf slight damage of the seedling that Bt soaks contrasts the leaf major injury of seedling simultaneously.

Obtain following LC ₅₀Value:

Strain is A:LC ₅₀=23.412ppm (change from 17.834 to 30.734)

Strain is B:LC ₅₀Plant immersion test (VIP3A) the 8.1 VIP3A albumen of=12.234ppm (change from 9.547 to 15.676) embodiment 8:Ostrinia furnacalis (Asiatic corn borer)

5mg VIP3A albumen prepares to prepare the variable concentrations of VIP3 albumen with the 50ml distilled water: 100ppm, 50ppm, 25ppm, 12.5ppm, 6.25ppm and 0ppm (contrast).8.2 Ostrinia furnacalis (Asiatic corn borer)

Collect pupa so that setup test is write down standards with larva (L2) 8.3 by entomology and the DOA of animal science portion from the farm that is positioned at Racha Buri

Cultivate after 5 days, collect data, then the percentage by the death of probability analysis process analysis larva by the quantity of calculating dead larva.8.4 method of testing

Use is to two inbred line plant of isozygotying (strain is B and C) of the corn (Zea mays) of Asiatic corn borer susceptible.Strain is that B is used for basin and carries plant test (4 groups of repeated experiments of 5 different gradient concentrations and contrast), and strain is that C is used for leaf Soak Test (100,50,4 groups of repeated experiments of 25ppm and contrast).The biggest seedling of 10-14 is soaked in the VIP3A of variable concentrations protein suspending liquid, and uses in feeding experiment, wherein larva be placed on doing of seedling leaf on, every plant 5-10 larva.Seedling covers with nylon net bag, and is put in the nylon wire cage.The leaf that downcuts and places in the control room 4 groups of repeated experiments of this test use in sealed plastic bag.8.4 result

Table 4: strain is the result of B

Table 4b

Dosage (ppm)	The insect sum	The dead insects number	Lethality (%)
				?100	????40	????31	????76.92
?50	????40	????26	????64.10
				?25	????40	????16	????38.46
?12.5	????40	????14	????33.33
				?6.25	????40	????9	????20.51
?0	????40	????1	????2.50

Table 4b

Repeat	Dead larva number after 5 days
							Concentration (ppm)
		Contrast	6.25	?12.5	???25	???50							??100
?Ⅰ(10)							????0	????3	????3	????5	????8	????6
	?Ⅱ(10)	????0	????2	????2	????3	????6							????9
?Ⅲ(10)							????1	????3	????5	????4	????5	????9
	?Ⅳ(10)	????0	????1	????4	????4	????7							????7
Sum							????1	????9	???14	???16	???26	???31

Table 5: strain is the result of C

Table 5a

Repeat	Dead larva number after 24 hours
					Concentration (ppm)
		Contrast	????25	????50					????100
????Ⅰ(10)					????0	????0	????0	????1
	????Ⅱ(10)	????0	????0	????0					????1
????Ⅲ(10)					????0	????0	????0	????0
	????Ⅳ(10)	????0	????0	????0					????1
Sum					????0	????0	????0	????3

Table 5b

Repeat	Dead larva number after 48 hours
					Concentration (ppm)
		Contrast	25	?50					?100
?Ⅰ(10)					????0	????0	????0	????1
	?Ⅱ(10)	????0	????0	????0					????1
?Ⅲ(10)					????0	????0	????1	????0
	?Ⅳ(10)	????0	????0	????0					????1
Sum					????0	????0	????1	????3

Table 5c

Repeat	Dead larva number after 72 hours
					Concentration (ppm)
		Contrast	???25	???50					??100
?Ⅰ(10)					????0	????1	????0	????2
	?Ⅱ(10)	????0	????1	????0					????1
?Ⅲ(10)					????0	????0	????2	????3
	?Ⅳ(10)	????0	????0	????2					????1
Sum					????0	????2	????4	????7

Table 5d

Repeat	Dead larva number concentration (ppm) after 96 hours
						Contrast	????25	????50	????100
?Ⅰ(10)	????0	????1	????1	????4
					?Ⅱ(10)	????0	????2	????1	????3
?Ⅲ(10)	????0	????1	????3	????5
					?Ⅳ(10)	????0	????1	????4	????3
Sum	????0	????5	????1	????15

Table 5e

Repeat	Dead larva number concentration (ppm) after 120 hours
						Contrast	????25	????50	????100
?Ⅰ(10)	????1	????3	????2	????7
					?Ⅱ(10)	????0	????2	????1	????4
?Ⅲ(10)	????0	????3	????5	????5
					?Ⅳ(10)	????0	????2	????4	????9
Sum	????1	????10	????12	????25

Obtain following LC after 120 hours ₅₀Value:

Strain is B:LC ₅₀=29.558ppm (change from 21.298 to 41.022)

Strain is C:LC ₅₀=74.498ppm (change from 53.644 to 114.866)

Sequence table

(1) essential information

(ⅰ) applicant

(A) title: Novartis AG

(B) street name: Schwarzwaldallee 215

(C) city: Basel

(D) country: Switzerland

(E) postcode (ZIP): 4002

(F) phone :+4161691111

(G) fax :+41616967976

(H) fax: 962991

(ⅱ) denomination of invention: the method for control insect pest

(ⅲ) sequence number: 55

(ⅳ) computer-reader form

(A) media type: floppy disk

(B) calculator: IBM PC compatible

(C) operating system: PC-DOS/MS-DOS

(D) software: PatentIn Release#1.0, version #1.30B

(2) about the information of SEQ ID NO:1

(ⅰ) sequence signature

(A) length: 6049 nucleotide

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: DNA (genome)

(ⅵ) primary source

(A) biology: Bacillus cercus

(B) bacterial strain: AB78

(C) particular separation thing: NRRL B-21058

(ⅸ) feature:

(A) title/key: CDS

(B) position: 1082.2467

(D) out of Memory :/product=" VIP2A (a) "

(ⅸ) feature:

(A) title/key: misc feature

(B) position: 2475..5126

(D) out of Memory: the coded sequence of/remarks=100kd VIP1A (a) albumen.This coded sequence repeats in SEQ ID NO:4 and translation respectively.

( ⅹⅰ ) ：SEQ ID NO:1：ATCGATACAA TGTTGTTTTA CTTAGACCGG TAGTCTCTGT AATTTGTTTA ATGCTATATT 60CTTTACTTTG ATACATTTTA ATAGCCATTT CAACCTTTAT AGTATGTTTT TGTGGTCTTC 120CTCCCTTTTT TCCACGAGCT CTAGCTGCGT TTAATCCTGT TTTGGTACGT TCGCTAATAA 180TATCTCTTTC TAATTCTGCA ATACTTGCCA TCATTCGAAA GAAGAATTTC CCCATAGCAT 240TAGAGGTATC AATGTTGTCA TGAATAGAAA TAAAATCTAC ACCTAGCTCT TTGAATTTTT 300CACTTAACTC AATTAGGTGT TTTGTAGAGC GAGAAATTCG ATCAAGTTTG TAAACAACTA 360TCTTATCGCC TTTACGTAAT ACTTTTAGCA ACTCTTCGAG TTGAGGGCGC TCTTTTTTTA 420TTCCTGTTAT TTTCTCCTGA TATAGCCTTT CTACACCATA TTGTTGCAAA GCATCTATTT 480GCATATCGAG ATTTTGTTCT TCTGTGCTGA CACGAGCATA ACCAAAAATC AAATTGGTTT 540CACTTCCTAT CTAAATATAT CTATTAAAAT AGCACCAAAA ACCTTATTAA ATTAAAATAA 600GGAACTTTGT TTTTGGATAT GGATTTTGGT ACTCAATATG GATGAGTTTT TAACGCTTTT 660GTTAAAAAAC AAACAAGTGC CATAAACGGT CGTTTTTGGG ATGACATAAT AAATAATCTG 720TTTGATTAAC CTAACCTTGT ATCCTTACAG CCCAGTTTTA TTTGTACTTC AACTGACTGA 780ATATGAAAAC AACATGAAGG TTTCATAAAA TTTATATATT TTCCATAACG GATGCTCTAT 840CTTTAGGTTA TAGTTAAATT ATAAGAAAAA AACAAACGGA GGGAGTGAAA AAAAGCATCT 900TCTCTATAAT TTTACAGGCT CTTTAATAAG AAGGGGGGAG ATTAGATAAT AAATATGAAT 960ATCTATCTAT AATTGTTTGC TTCTACAATA ACTTATCTAA CTTTCATATA CAACAACAAA 1020ACAGACTAAA TCCAGATTGT ATATTCATTT TCAGTTGTTC CTTTATAAAA TAATTTCATA 1080A ATG AAA AGA ATG GAG GGA AAG TTG TTT ATG GTG TCA AAA AAA TTA 1126 Met Lys Arg Met Glu Gly Lys Leu Phe Met Val Ser Lys Lys Leu

1???????????????5??????????????????10??????????????????15CAA?GTA?GTT?ACT?AAA?ACT?GTA?TTG?CTT?AGT?ACA?GTT?TTC?TCT?ATA?TCT??????1174Gln?Val?Val?Thr?Lys?Thr?Val?Leu?Leu?Ser?Thr?Val?Phe?Ser?Ile?Ser

20??????????????????25??????????????????30TTA?TTA?AAT?AAT?GAA?GTG?ATA?AAA?GCT?GAA?CAA?TTA?AAT?ATA?AAT?TCT??????1222Leu?Leu?Asn?Asn?Glu?Val?Ile?Lys?Ala?Glu?Gln?Leu?Asn?Ile?Asn?Ser

35??????????????????40??????????????????45CAA?AGT?AAA?TAT?ACT?AAC?TTG?CAA?AAT?CTA?AAA?ATC?ACT?GAC?AAG?GTA??????1270Gln?Ser?Lys?Tyr?Thr?Asn?Leu?Gln?Asn?Leu?Lys?Ile?Thr?Asp?Lys?Val

50??????????????????55??????????????????60GAG?GAT?TTT?AAA?GAA?GAT?AAG?GAA?AAA?GCG?AAA?GAA?TGG?GGG?AAA?GAA??????1318Glu?Asp?Phe?Lys?Glu?Asp?Lys?Glu?Lys?Ala?Lys?Glu?Trp?Gly?Lys?Glu

65??????????????????70??????????????????75AAA?GAA?AAA?GAG?TGG?AAA?CTA?ACT?GCT?ACT?GAA?AAA?GGA?AAA?ATG?AAT??????1366Lys?Glu?Lys?Glu?Trp?Lys?Leu?Thr?Ala?Thr?Glu?Lys?Gly?Lys?Met?Asn?80??????????????????85??????????????????90??????????????????95AAT?TTT?TTA?GAT?AAT?AAA?AAT?GAT?ATA?AAG?ACA?AAT?TAT?AAA?GAA?ATT??????1414ASn?Phe?Leu?Asp?Asn?Lys?Asn?Asp?Ile?Lys?Thr?Asn?Tyr?Lys?Glu?Ile

100?????????????????105?????????????????110ACT?TTT?TCT?ATG?GCA?GGC?TCA?TTT?GAA?GAT?GAA?ATA?AAA?GAT?TTA?AAA??????1462Thr?Phe?SER?Met?Ala?Gly?Ser?Phe?Glu?Asp?Glu?Ile?Lys?Asp?Leu?Lys

115?????????????????120?????????????????125GAA?ATT?GAT?AAG?ATG?TTT?GAT?AAA?ACC?AAT?CTA?TCA?AAT?TCT?ATT?ATC??????1510Glu?Ile?Asp?Lys?Met?Phe?Asp?Lys?Thr?Asn?Leu?Ser?Asn?Ser?Ile?Ile

130?????????????????135?????????????????140ACC?TAT?AAA?AAT?GTG?GAA?CCG?ACA?ACA?ATT?GGA?TTT?AAT?AAA?TCT?TTA??????1558Thr?Tyr?Lys?Asn?Val?Glu?Pro?Thr?Thr?Ile?Gly?Phe?Asn?Lys?Ser?Leu

145?????????????????150?????????????????155ACA?GAA?GGT?AAT?ACG?ATT?AAT?TCT?GAT?GCA?ATG?GCA?CAG?TTT?AAA?GAA??????1606Thr?Glu?Gly?Asn?Thr?Ile?Asn?Ser?Asp?Ala?Met?Ala?Gln?Phe?Lys?Glu160?????????????????165?????????????????170?????????????????175CAA?TTT?TTA?GAT?AGG?GAT?ATT?AAG?TTT?GAT?AGT?TAT?CTA?GAT?ACG?CAT??????1654Gln?Phe?Leu?ASp?Arg?Asp?Ile?Lys?Phe?Asp?Ser?Tyr?Leu?Asp?Thr?His

180?????????????????185?????????????????190TTA?ACT?GCT?CAA?CAA?GTT?TCC?AGT?AAA?GAA?AGA?GTT?ATT?TTG?AAG?GTT??????1702Leu?Thr?Ala?Gln?Gln?Val?Ser?Ser?Lys?Glu?Arg?Val?Ile?Leu?Lys?Val

195?????????????????200?????????????????205ACG?GTT?CCG?AGT?GGG?AAA?GGT?TCT?ACT?ACT?CCA?ACA?AAA?GCA?GGT?GTC??????1750Thr?Val?Pro?Ser?Gly?Lys?Gly?Ser?Thr?Thr?Pro?Thr?Lys?Ala?Gly?Val

210?????????????????215?????????????????220ATT?TTA?AAT?AAT?AGT?GAA?TAC?AAA?ATG?CTC?ATT?GAT?AAT?GGG?TAT?ATG??????1798Ile?Leu?Asn?Asn?Ser?Glu?Tyr?Lys?Met?Leu?Ile?Asp?Asn?Gly?Tyr?Met

225?????????????????230?????????????????235GTC?CAT?GTA?GAT?AAG?GTA?TCA?AAA?GTG?GTG?AAA?AAA?GGG?GTG?GAG?TGC??????1846Val?His?Val?Asp?Lys?Val?Ser?Lys?Val?Val?Lys?Lys?Gly?Val?Glu?Cys240?????????????????245?????????????????250?????????????????255TTA?CAA?ATT?GAA?GGG?ACT?TTA?AAA?AAG?AGT?CTT?GAC?TIT?AAA?AAT?GAT??????1894Leu?Gln?Ile?Glu?Gly?Thr?Leu?Lys?Lys?Ser?Leu?Asp?Phe?Lys?Asn?Asp

260?????????????????265?????????????????270ATA?AAT?GCT?GAA?GCG?CAT?AGC?TGG?GGT?ATG?AAG?AAT?TAT?GAA?GAG?TGG??????1942Ile?Asn?Ala?Glu?Ala?His?Ser?Trp?Gly?Met?Lys?Asn?Tyr?Glu?Glu?Trp

275?????????????????280?????????????????285GCT?AAA?GAT?TTA?ACC?GAT?TCG?CAA?AGG?GAA?GCT?TPA?GAT?GGG?TAT?GCT??????1990Ala?Lys?Asp?Leu?Thr?Asp?Ser?Gln?Arg?Glu?Ala?Leu?Asp?Gly?Tyr?Ala

290?????????????????295?????????????????300AGG?CAA?GAT?TAT?AAA?GAA?ATC?AAT?AAT?TAT?TTA?AGA?AAT?CAA?GGC?GGA??????2038Arg?Gln?Asp?Tyr?Lys?Glu?Ile?Asn?Asn?Tyr?Leu?Arg?Asn?Gln?Gly?Gly

305?????????????????310?????????????????315AGT?GGA?AAT?GAA?AAA?CTA?GAT?GCT?CAA?ATA?AAA?AAT?ATT?TCT?GAT?GCT??????2086Ser?Gly?Asn?Glu?Lys?Leu?Asp?Ala?Gln?Ile?Lys?Asn?Ile?Ser?Asp?Ala320?????????????????325?????????????????330?????????????????335TTA?GGG?AAG?AAA?CCA?ATA?CCG?GAA?AAT?ATT?ACT?GTG?TAT?AGA?TGG?TGT??????2134Leu?Gly?Lys?Lys?Pro?Ile?Pro?Glu?Asn?Ile?Thr?Val?Tyr?Arg?Trp?Cys

340?????????????????345?????????????????????350GGC?ATG?CCG?GAA?TIT?GGT?TAT?CAA?ATT?AGT?GAT?CCG?TTA?CCT?TCT?TTA??????2182Gly?Met?Pro?Glu?Phe?Gly?Tyr?Gln?Ile?Ser?Asp?Pro?Leu?Pro?Ser?Leu

355?????????????????360?????????????????????365AAA?GAT?TTT?GAA?GAA?CAA?TTT?TTA?AAT?ACA?ATC?AAA?GAA?GAC?AAA?GGA??????2230Lys?Asp?Phe?Glu?Glu?Gln?Phe?Leu?Asn?Thr?Ile?Lys?Glu?Asp?Lys?Gly

370?????????????????375?????????????????????380TAT?ATG?AGT?ACA?AGC?TTA?TCG?AGT?GAA?CGT?CTT?GCA?GCT?TIT?GGA?TCT??????2278Tyr?Met?Ser?Thr?Ser?Leu?Ser?Ser?Glu?Arg?Leu?Ala?Ala?Phe?Gly?Ser

385?????????????????390?????????????????????395AGA?AAA?ATT?ATA?TTA?CGA?TTA?CAA?GTT?CCG?AAA?GGA?AGT?ACG?GGT?GCG??????2326Arg?Lys?Ile?Ile?Leu?Arg?Leu?Gln?Val?Pro?Lys?Gly?Ser?Thr?Gly?Ala400?????????????????405?????????????????????410?????????????415TAT?TTA?AGT?GCC?ATT?GGT?GGA?TTT?GCA?AGT?GAA?AAA?GAG?ATC?CTA?CTT??????2374Tyr?Leu?Ser?Ala?Ile?Gly?Gly?Phe?Ala?Ser?Glu?Lys?Glu?Ile?Leu?Leu

420?????????????????????425?????????????430GAT?AAA?GAT?AGT?AAA?TAT?CAT?ATT?GAT?AAA?GTA?ACA?GAG?GTA?ATT?ATT??????2422Asp?Lys?Asp?Ser?Lys?Tyr?His?Ile?Asp?Lys?Val?Thr?Glu?Val?Ile?Ile

435?????????????????440?????????????????445AAA?GGT?GTT?AAG?CGA?TAT?GTA?GTG?GAT?GCA?ACA?TTA?TTA?ACA?AAT??????????2467Lys?Gly?Val?Lys?Arg?Tyr?Val?Val?Asp?Ala?Thr?Leu?Leu?Thr?Asn

450?????????????????455?????????????????460TAAGGAGATG?AAAAATATGA?AGAAAAAGTT?AGCAAGTGTT?GTAACGTGTA?CGTTATTAGC????2527TCCTATGTTT?TTGAATGGAA?ATGTGAATGC?TGTTTACGCA?GACAGCAAAA?CAAATCAAAT????2587TTCTACAACA?CAGAAAAATC?AACAGAAAGA?GATGGACCGA?AAAGGATTAC?TTGGGTATTA????2647TTTCAAAGGA?AAAGATTTTA?GTAATCTTAC?TATGTTTGCA?CCGACACGTG?ATAGTACTCT????2707TATTTATGAT?CAACAAACAG?CAAATAAACT?ATTAGATAAA?AAACAACAAG?AATATCAGTC????2767TATTCGTTGG?ATTGGTTTGA?TTCAGAGTAA?AGAAACGGGA?GATTTCACAT?TTAACTTATC????2827TGAGGATGAA?CAGGCAATTA?TAGAAATCAA?TGGGAAAATT?ATTTCTAATA?AAGGGAAAGA????2887AAAGCAAGTT?GTCCATTTAG?AAAAAGGAAA?ATTAGTTCCA?ATCAAAATAG?AGTATCAATC????2947AGATACAAAA?TTTAATATTG?ACAGTAAAAC?ATTTAAAGAA?CTTAAATTAT?TTAAAATAGA????3007TAGTCAAAAC?CAACCCCAGC?AAGTCCAGCA?AGATGAACTG?AGAAATCCTG?AATTTAACAA????3067GAAAGAATCA?CAGGAATTCT?TAGCGAAACC?ATCGAAAATA?AATCTTTTCA?CTCAAAAAAT????3127GAAAAGGGAA?ATTGATGAAG?ACACGGATAC?GGATGGGGAC?TCTATTCCTG?ACCTTTGGGA????3187AGAAAATGGG?TATACGATTC?ACAATAGAAT?CGCTGTAAAG?TGGGACGATT?CTCTAGCAAG????3247TAAAGGGTAT?ACGAAATTTG?TTTCAAATCC?ACTAGAAAGT?CACACAGTTG?GTGATCCTTA????3307TACAGATTAT?GAAAAGGCAG?CAAGAGATCT?AGATTTGTCA?AATGCAAAGG?AAACGTTTAA????3367CCCATTGGTA?GCTGCTTTTC?CAAGTGTGAA?TGTTAGTATG?GAAAAGGTGA?TATTATCACC????3427AAATGAAAAT?TTATCCAATA?GTGTAGAGTC?TCATTCATCC?ACGAATTGGT?CTTATACAAA????3487TACAGAAGGT?GCTTCTGTTG?AAGCGGGGAT?TGGACCAAAA?GGTATTTCGT?TCGGAGTTAG????3547CGTAAACTAT?CAACACTCTG?AAACAGTTGC?ACAAGAATGG?GGAACATCTA?CAGGAAATAC????3607TTCGCAATTC?AATACGGCTT?CAGCGGGATA?TTTAAATGCA?AATGTTCGAT?ATAACAATGT????3667AGGAACTGGT?GCCATCTACG?ATGTAAAACC?TACAACAAGT?TTTGTATTAA?ATAACGATAC????3727TATCGCAACT?ATTACGGCGA?AATCTAATTC?TACAGCCTTA?AATATATCTC?CTGGAGAAAG????3787TTACCCGAAA?AAAGGACAAA?ATGGAATCGC?AATAACATCA?ATGGATGATT?TTAATTCCCA????3847TCCGATTACA?TTAAATAAAA?AACAAGTAGA?TAATCTGCTA?AATAATAAAC?CTATGATGTT????3907GGAAACAAAC?CAAACAGATG?GTGTTTATAA?GATAAAAGAT?ACACATGGAA?ATATAGTAAC????3967TGGCGGAGAA?TGGAATGGTG?TCATACAACA?AATCAAGGCT?AAAACAGCGT?CTATTATTGT????4027GGATGATGGG?GAACGTGTAG?CAGAAAAACG?TGTAGCGGCA?AAAGATTATG?AAAATCCAGA????4087AGATAAAACA?CCGTCTTTAA?CTTTAAAAGA?TGCCCTGAAG?CTTTCATATC?CAGATGAAAT????4147AAAAGAAATA?GAGGGATTAT?TATATTATAA?AAACAAACCG?ATATACGAAT?CGAGCGTTAT????4207GACTTACTTA?GATGAAAATA?CAGCAAAAGA?AGTGACCAAA?CAATTAAATG?ATACCACTGG????4267GAAATTTAAA?GATGTAAGTC?ATTTATATGA?TGTAAAACTG?ACTCCAAAAA?TGAATGTTAC????4327AATCAAATTG?TCTATACTTT?ATGATAATGC?TGAGTCTAAT?GATAACTCAA?TTGGTAAATG????4387GACAAACACA?AATATTGTTT?CAGGTGGAAA?TAACGGAAAA?AAACAATATT?CTTCTAATAA????4447TCCGGATGCT?AATTTGACAT?TAAATACAGA?TGCTCAAGAA?AAATTAAATA?AAAATCGTGA????4507CTATTATATA?AGTTTATATA?TGAAGTCAGA?AAAAAACACA?CAATGTGAGA?TTACTATAGA????4567TGGGGAGATT?TATCCGATCA?CTACAAAAAC?AGTGATGTTG?AATAAAGACA?ATTACAAAAG????4627ATTAGATATT?ATAGCTCATA?ATATAAAAAG?TAATCCAATT?TCTTCACTTC?ATATTAAAAC????4687GAATGATGAA?ATAACTTTAT?TTTGGGATGA?TATTTCTATA?ACAGATGTAG?CATCAATAAA????4747ACCGGAAAAT?TTAACAGATT?CAGAAATTAA?ACAGATTTAT?AGTAGGTATG?GTATTAAGTT????4807AGAAGATGGA?ATCCTTATTG?ATAAAAAAGG?TGGGATTCAT?TATGGTGAAT?TTATTAATGA????4867AGCTAGTTTT?AATATTGAAC?CATTGCAAAA?TTATGTGACC?AAATATGAAG?TTACTTATAG????4927TAGTGAGTTA?GGACCAAACG?TGAGTGACAC?ACTTGAAAGT?GATAAAATTT?ACAAGGATGG????4987GACAATTAAA?TTTGATTTTA?CCAAATATAG?TAAAAATGAA?CAAGGATTAT?TTTATGACAG????5047TGGATTAAAT?TGGGACTTTA?AAATTAATGC?TATTACTTAT?GATGGTAAAG?AGATGAATGT????5107TTTTCATAGA?TATAATAAAT?AGTTATTATA?TCTATGAAGC?TGGTGCTAAA?GATAGTGTAA????5167AAGTTAATAT?ACTGTAGGAT?TGTAATAAAA?GTAATGGAAT?TGATATCGTA?CTTTGGAGTG????5227GGGGATACTT?TGTAAATAGT?TCTATCAGAA?ACATTAGACT?AAGAAAAGTT?ACTACCCCCA????5287CTTGAAAATG?AAGATTCAAC?TGATTACAAA?CAACCTGTTA?AATATTATAA?GGTTTTAACA????5347AAATATTAAA?CTCTTTATGT?TAATACTGTA?ATATAAAGAG?TTTAATTGTA?TTCAAATGAA????5407GCTTTCCCAC?AAAATTAGAC?TGATTATCTA?ATGAAATAAT?CAGTCTAATT?TTGTAGAACA????5467GGTCTGGTAT?TATTGTACGT?GGTCACTAAA?AGATATCTAA?TATTATTGGG?CAAGGCGTTC????5527CATGATTGAA?TCCTCGAATG?TCTTGCCCTT?TTCATTTATT?TAAGAAGGAT?TGTGGAGAAA????5587TTATGGTTTA?GATAATGAAG?AAAGACTTCA?CTTCTAATTT?TTGATGTTAA?ATAAATCAAA????5647ATTTGGCGAT?TCACATTGTT?TAATCCACTG?ATAAAACATA?CTGGAGTGTT?CTTAAAAAAT????5707CAGCTTTTTT?CTTTATAAAA?TTTTGCTTAG?CGTACGAAAT?TCGTGTTTTG?TTGGTGGGAC????5767CCCATGCCCA?TCAACTTAAG?AGTAAATTAG?TAATGAACTT?TCGTTCATCT?GGATTAAAAT????5827AACCTCAAAT?TAGGACATGT?TTTTAAAAAT?AAGCAGACCA?AATAAGCCTA?GAATAGGTAT????5887CATTTTTAAA?AATTATGCTG?CTTTCTTTTG?TTTTCCAAAT?CCATTATACT?CATAAGCAAC????5947ACCCATAATG?TCAAAGACTG?TTTTTGTCTC?ATATCGATAA?CCTTGATATC?GAATTCCTGC????6007AGCCCGGGGG?ATCCACTAGT?TCTAGAGCGG?CCGCCACCGC?GG???????????????????????6049

(2) about the information of SEQ ID NO:2

(ⅰ) sequence signature

(A) length: 462 amino acid

(B) type: amino acid

(D) topological structure: line style

(ⅱ) molecule type: protein

(ⅹ ⅰ) sequence description: SEQ ID NO:2:Met Lys Arg Met Glu Gly Lys Leu Phe Met Val Ser Lys Lys Leu Gln 15 10 15Val Val Thr Lys Thr Val Leu Leu Ser Thr Val Phe Ser Ile Ser Leu

20??????????????????25??????????????????30Leu?Asn?Asn?Glu?Val?Ile?Lys?Ala?Glu?Gln?Leu?Asn?Ile?Asn?Ser?Gln

35??????????????????40??????????????????45Ser?Lys?Tyr?Thr?Asn?Leu?Gln?Asn?Leu?Lys?Ile?Thr?Asp?Lys?Val?Glu

50??????????????????55??????????????????60Asp?Phe?Lys?Glu?Asp?Lys?Glu?Lys?Ala?Lys?Glu?Trp?Gly?Lys?Glu?Lys?65??????????????????70??????????????????75??????????????????80Glu?Lys?Glu?Trp?Lys?Leu?Thr?Ala?Thr?Glu?Lys?Gly?Lys?Met?Asn?Asn

85??????????????????90??????????????????95Phe?Leu?Asp?Asn?Lys?Asn?Asp?Ile?Lys?Thr?Asn?Tyr?Lys?Glu?Ile?Thr

100?????????????????105?????????????????110Phe?Ser?Met?Ala?Gly?Ser?Phe?Glu?Asp?Glu?Ile?Lys?Asp?Leu?Lys?Glu

115?????????????????120?????????????????125Ile?Asp?Lys?Met?Phe?Asp?Lys?Thr?Asn?Leu?Ser?Asn?Ser?Ile?Ile?Thr

130?????????????????135?????????????????140Tyr?Lys?Asn?Val?Glu?Pro?Thr?Thr?Ile?Gly?Phe?Asn?Lys?Ser?Leu?Thr145?????????????????150?????????????????155?????????????????160Glu?Gly?Asn?Thr?Ile?Asn?Ser?Asp?Ala?Met?Ala?Gln?Phe?Lys?Glu?Gln

165?????????????????170?????????????175Phe?Leu?Asp?Arg?Asp?Ile?Lys?Phe?Asp?Ser?Tyr?Leu?Asp?Thr?His?Leu

180?????????????????185?????????????????190Thr?Ala?Gln?Gln?Val?Ser?Ser?Lys?Glu?Arg?Val?Ile?Leu?Lys?Val?Thr

195?????????????????200?????????????????205Val?Pro?Ser?Gly?Lys?Gly?Ser?Thr?Thr?Pro?Thr?Lys?Ala?Gly?Val?Ile

210?????????????????215?????????????????220Leu?Asn?Asn?Ser?Glu?Tyr?Lys?Met?Leu?Ile?Asp?Asn?Gly?Tyr?Met?Val225?????????????????230?????????????????235?????????????????240His?Val?Asp?Lys?Val?Ser?Lys?Val?Val?Lys?Lys?Gly?Val?Glu?Cys?Leu

245?????????????????250?????????????????255Gln?Ile?Glu?Gly?Thr?Leu?Lys?Lys?Ser?Leu?Asp?Phe?Lys?Asn?Asp?Ile

260?????????????????265?????????????????270Asn?Ala?Glu?Ala?His?Ser?Trp?Gly?Met?Lys?Asn?Tyr?Glu?Glu?Trp?Ala

275?????????????????280?????????????????285Lys?Asp?Leu?Thr?Asp?Ser?Gln?Arg?Glu?Ala?Leu?Asp?Gly?Tyr?Ala?Arg

290?????????????????295?????????????????300Gln?Asp?Tyr?Lys?Glu?Ile?Asn?Asn?Tyr?Leu?Arg?Asn?Gln?Gly?Gly?Ser305?????????????????310?????????????????315?????????????????320Gly?Asn?Glu?Lys?Leu?Asp?Ala?Gln?Ile?Lys?Asn?Ile?Ser?Asp?Ala?Leu

325?????????????????330?????????????????335Gly?Lys?Lys?Pro?Ile?Pro?Glu?Asn?Ile?Thr?Val?Tyr?Arg?Trp?Cys?Gly

340?????????????????345?????????????????350Met?Pro?Glu?Phe?Gly?Tyr?Gln?Ile?Ser?Asp?Pro?Leu?Pro?Ser?Leu?Lys

355?????????????????360?????????????????365Asp?Phe?Glu?Glu?Gln?Phe?Leu?Asn?Thr?Ile?Lys?Glu?Asp?Lys?Gly?Tyr

370?????????????????375?????????????????380Met?Ser?Thr?Ser?Leu?Ser?Ser?Glu?Arg?Leu?Ala?Ala?Phe?Gly?Ser?Arg385?????????????????390?????????????????395?????????????????400Lys?Ile?Ile?Leu?Arg?Leu?Gln?Val?Pro?Lys?Gly?Ser?Thr?Gly?Ala?Tyr

405?????????????????410?????????????????415Leu?Ser?Ala?Ile?Gly?Gly?Phe?Ala?Ser?Glu?Lys?Glu?Ile?Leu?Leu?Asp

420?????????????????425?????????????????430Lys?Asp?Ser?Lys?Tyr?His?Ile?Asp?Lys?Val?Thr?Glu?Val?Ile?Ile?Lys

435?????????????????440?????????????????445Gly?Val?Lys?Arg?Tyr?Val?Val?Asp?Ala?Thr?Leu?Leu?Thr?Asn

450?????????????????455?????????????????460

(2) about the information of SEQ ID NO:3

(ⅰ) sequence signature

(A) length: 20 amino acid

(B) type: amino acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: peptide

(ⅸ) feature:

(A) title/key: peptide

(B) position: 1..20

(D) out of Memory: lead signal peptide (ⅹ ⅰ) sequence description of vacuole of/remarks=be used to: SEQ ID NO:3:Ser Ser Ser Ser Phe Ala Asp Ser Asn Pro Ile Arg Val Thr Asp Arg1 5 10 15Ala Ala Ser Thr

20

(2) about the information of SEQ ID NO:4

(ⅰ) sequence signature

(A) length: 2655 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: DNA (genome)

(ⅲ) the false plan: not

(ⅳ) antisense: not

(ⅵ) primary source

(A) biology: Bacillus cercus

(B) bacterial strain: AB78

(C) particular separation thing: NRRL B-21058

(ⅸ) feature:

(A) title/key: CDS

(B) position: 1..2652

(D) out of Memory :/product=" 100kDa VIP1A (a) albumen " ,/remarks=" this sequence is identical with from 2475 to 5126 the part nucleotide of SEQ ID NO:1.”

(ⅹ ⅰ) sequence description: SEQ ID NO:4:ATG AAA AAT ATG AAG AAA AAG TTA GCA AGT GTT GTA ACG TGT ACG TTA 48Met Lys Asn Met Lys Lys Lys Leu Ala Ser Val Val Thr Cys Thr Leu

465?????????????????470?????????????????475TTA?GCT?CCT?ATG?TTT?TTG?AAT?GGA?AAT?GTG?AAT?GCT?GTT?TAC?GCA?GAC?????????96Leu?Ala?Pro?Met?Phe?Leu?Asn?Gly?Asn?Val?Asn?Ala?Val?Tyr?Ala?Asp

480?????????????????485?????????????????490AGC?AAA?ACA?AAT?CAA?ATT?TCT?ACA?ACA?CAG?AAA?AAT?CAA?CAG?AAA?GAG?????????144Ser?Lys?Thr?Asn?Gln?Ile?Ser?Thr?Thr?Gln?Lys?Asn?Gln?Gln?Lys?Glu495?????????????????500?????????????????505?????????????????510ATG?GAC?CGA?AAA?GGA?TTA?CTT?GGG?TAT?TAT?TTC?AAA?GGA?AAA?GAT?TTT?????????192Met?Asp?Arg?Lys?Gly?Leu?Leu?Gly?Tyr?Tyr?Phe?Lys?Gly?Lys?Asp?Phe

515?????????????????520?????????????????525AGT?AAT?CTT?ACT?ATG?TTT?GCA?CCG?ACA?CGT?GAT?AGT?ACT?CTT?ATT?TAT?????????240Ser?Asn?Leu?Thr?Met?Phe?Ala?Pro?Thr?Arg?Asp?Ser?Thr?Leu?Ile?Tyr

530?????????????????535?????????????????540GAT?CAA?CAA?ACA?GCA?AAT?AAA?CTA?TTA?GAT?AAA?AAA?CAA?CAA?GAA?TAT?????????288Asp?Gln?Gln?Thr?Ala?Asn?Lys?Leu?Leu?Asp?Lys?Lys?Gln?Gln?Glu?Tyr

545?????????????????550?????????????????555CAG?TCT?ATT?CGT?TGG?ATT?GGT?TTG?ATP?CAG?AGT?AAA?GAA?ACG?GGA?GAT?????????336Gln?Ser?Ile?Arg?Trp?Ile?Gly?Leu?Ile?Gln?Ser?Lys?Glu?Thr?Gly?Asp

560?????????????????565?????????????????570TTC?ACA?TTT?AAC?TTA?TCT?GAG?GAT?GAA?CAG?GCA?ATT?ATA?GAA?ATC?AAT?????????384Phe?Thr?Phe?Asn?Leu?Ser?Glu?Asp?Glu?Gln?Ala?Ile?Ile?Glu?Ile?Asn575?????????????????580?????????????????585?????????????????590GGG?AAA?ATT?ATT?TCT?AAT?AAA?GGG?AAA?GAA?AAG?CAA?GTT?GTC?CAT?TTA?????????432Gly?Lys?Ile?Ile?Ser?Asn?Lys?Gly?Lys?Glu?Lys?Gln?Val?Val?His?Leu

595?????????????????600?????????????????605GAA?AAA?GGA?AAA?TTA?GTT?CCA?ATC?AAA?ATA?GAG?TAT?CAA?TCA?GAT?ACA?????????480Glu?Lys?Gly?Lys?Leu?Val?Pro?Ile?Lys?Ile?Glu?Tyr?Gln?Ser?Asp?Thr

610?????????????????615?????????????????620AAA?TTT?AAT?ATT?GAC?AGT?AAA?ACA?TTT?AAA?GAA?CTT?AAA?TTA?TTT?AAA?????????528Lys?Phe?Asn?Ile?Asp?Ser?Lys?Thr?Phe?Lys?Glu?Leu?Lys?Leu?Phe?Lys

625?????????????????630?????????????????635ATA?GAT?AGT?CAA?AAC?CAA?CCC?CAG?CAA?GTC?CAG?CAA?GAT?GAA?CTG?AGA?????????576Ile?Asp?Ser?Gln?Asn?Gln?Pro?Gln?Gln?Val?Gln?Gln?Asp?Glu?Leu?Arg

640?????????????????645?????????????????650AAT?CCT?GAA?TTT?AAC?AAG?AAA?GAA?TCA?CAG?GAA?TTC?TTA?GCG?AAA?CCA?????????624Asn?Pro?Glu?Phe?Asn?Lys?Lys?Glu?Ser?Gln?Glu?Phe?Leu?Ala?Lys?Pro655?????????????????660?????????????????665?????????????????670TCG?AAA?ATA?AAT?CTT?TTC?ACT?CAA?AAA?ATG?AAA?AGG?GAA?ATT?GAT?GAA?????????672Ser?Lys?Ile?Asn?Leu?Phe?Thr?Gln?Lys?Met?Lys?Arg?Glu?Ile?Asp?Glu

675?????????????????680?????????????????685GAC?ACG?GAT?ACG?GAT?GGG?GAC?TCT?ATT?CCT?GAC?CTT?TGG?GAA?GAA?AAT????????720Asp?Thr?Asp?Thr?Asp?Gly?Asp?Ser?Ile?Pro?Asp?Leu?Trp?Glu?Glu?Asn

690?????????????????695?????????????????700GGG?TAT?ACG?ATT?CAA?AAT?AGA?ATC?GCT?GTA?AAG?TGG?GAC?GAT?TCT?CTA????????768Gly?Tyr?Thr?Ile?Gln?Asn?Arg?Ile?Ala?Val?Lys?Trp?Asp?Asp?Ser?Leu

705?????????????????710?????????????????715GCA?AGT?AAA?GGG?TAT?ACG?AAA?TTT?GTT?TCA?AAT?CCA?CTA?GAA?AGT?CAC????????816Ala?Ser?Lys?Gly?Tyr?Thr?Lys?Phe?Val?Ser?Asn?Pro?Leu?Glu?Ser?His

720?????????????????725?????????????????730ACA?GTT?GGT?GAT?CCT?TAT?ACA?GAT?TAT?GAA?AAG?GCA?GCA?AGA?GAT?CTA????????864Thr?Val?Gly?Asp?Pro?Tyr?Thr?Asp?Tyr?Glu?Lys?Ala?Ala?Arg?Asp?Leu735?????????????????740?????????????????745?????????????????750GAT?TTG?TCA?AAT?GCA?AAG?GAA?ACG?TTT?AAC?CCA?TTG?GTA?GCT?GCT?TTT????????912Asp?Leu?Ser?Asn?Ala?Lys?Glu?Thr?Phe?Asn?Pro?Leu?Val?Ala?Ala?Phe

755?????????????????760?????????????????765CCA?AGT?GTG?AAT?GTT?AGT?ATG?GAA?AAG?GTG?ATA?TTA?TCA?CCA?AAT?GAA????????960Pro?Ser?Val?Asn?Val?Ser?Met?Glu?Lys?Val?Ile?Leu?Ser?Pro?Asn?Glu

770?????????????????775?????????????????780AAT?TTA?TCC?AAT?AGT?GTA?GAG?TCT?CAT?TCA?TCC?ACG?AAT?TGG?TCT?TAT???????1008Asn?Leu?Ser?Asn?Ser?Val?Glu?Ser?His?Ser?Ser?Thr?Asn?Trp?Ser?Tyr

785?????????????????790?????????????????795ACA?AAT?ACA?GAA?GGT?GCT?TCT?GTT?GAA?GCG?GGG?ATT?GGA?CCA?AAA?GGT???????1056Thr?Asn?Thr?Glu?Gly?Ala?Ser?Val?Glu?Ala?Gly?Ile?Gly?Pro?Lys?Gly

800?????????????????805?????????????????810ATT?TCG?TTC?GGA?GTT?AGC?GTA?AAC?TAT?CAA?CAC?TCT?GAA?ACA?GTT?GCA???????1104Ile?Ser?Phe?Gly?Val?Ser?Val?Asn?Tyr?Gln?His?Ser?Glu?Thr?Val?Ala815?????????????????820?????????????????825?????????????????830CAA?GAA?TGG?GGA?ACA?TCT?ACA?GGA?AAT?ACT?TCG?CAA?TTC?AAT?ACG?GCT???????1152Gln?Glu?Trp?Gly?Thr?Ser?Thr?Gly?Asn?Thr?Ser?Gln?Phe?Asn?Thr?Ala

835?????????????????840?????????????????845TCA?GCG?GGA?TAT?TTA?AAT?GCA?AAT?GTT?CGA?TAT?AAC?AAT?GTA?GGA?ACT???????1200Ser?Ala?Gly?Tyr?Leu?Asn?Ala?Asn?Val?Arg?Tyr?Asn?Asn?Val?Gly?Thr

850?????????????????855?????????????????860GGT?GCC?ATC?TAC?GAT?GTA?AAA?CCT?ACA?ACA?AGT?TTT?GTA?TTA?AAT?AAC???????1248Gly?Ala?Ile?Tyr?Asp?Val?Lys?Pro?Thr?Thr?Ser?Phe?Val?Leu?Asn?Asn

865?????????????????870?????????????????875GAT?ACT?ATC?GCA?ACT?ATT?ACG?GCG?AAA?TCT?AAT?TCT?ACA?GCC?TTA?AAT???????1296Asp?Thr?Ile?Ala?Thr?Ile?Thr?Ala?Lys?Ser?Asn?Ser?Thr?Ala?Leu?Asn

880?????????????????885?????????????????890ATA?TCT?CCT?GGA?GAA?AGT?TAC?CCG?AAA?AAA?GGA?CAA?AAT?GGA?ATC?GCA???????1344Ile?Ser?Pro?Gly?Glu?Ser?Tyr?Pro?Lys?Lys?Gly?Gln?Asn?Gly?Ile?Ala895?????????????????900?????????????????905?????????????????910ATA?ACA?TCA?ATG?GAT?GAT?TTT?AAT?TCC?CAT?CCG?ATT?ACA?TTA?AAT?AAA???????1392Ile?Thr?Ser?Met?Asp?Asp?Phe?Asn?Ser?His?Pro?Ile?Thr?Leu?Asn?Lys

915?????????????????920?????????????????925AAA?CAA?GTA?GAT?AAT?CTG?CTA?AAT?AAT?AAA?CCT?ATG?ATG?TTG?GAA?ACA???????1440Lys?Gln?Val?Asp?Asn?Leu?Leu?Asn?Asn?Lys?Pro?Met?Met?Leu?Glu?Thr

930?????????????????935?????????????????940AAC?CAA?ACA?GAT?GGT?GTT?TAT?AAG?ATA?AAA?GAT?ACA?CAT?GGA?AAT?ATA???????1488Asn?Gln?Thr?Asp?Gly?Val?Tyr?Lys?Ile?Lys?Asp?Thr?His?Gly?Asn?Ile

945?????????????????950?????????????????955GTA?ACT?GGC?GGA?GAA?TGG?AAT?GGT?GTC?ATA?CAA?CAA?ATC?AAG?GCT?AAA???????1536Val?Thr?Gly?Gly?Glu?Trp?Asn?Gly?Val?Ile?Gln?Gln?Ile?Lys?Ala?Lys

960?????????????????965?????????????????970ACA?GCG?TCT?ATT?ATT?GTG?GAT?GAT?GGG?GAA?CGT?GTA?GCA?GAA?AAA?CGT???????1584Thr?Ala?Ser?Ile?Ile?Val?Asp?Asp?Gly?Glu?Arg?Val?Ala?Glu?Lys?Arg975?????????????????980?????????????????985?????????????????990GTA?GCG?GCA?AAA?GAT?TAT?GAA?AAT?CCA?GAA?GAT?AAA?ACA?CCG?TCT?TTA???????1632Val?Ala?Ala?Lys?Asp?Tyr?Glu?Asn?Pro?Glu?Asp?Lys?Thr?Pro?Ser?Leu

995?????????????????1000????????????????1005ACT?TTA?AAA?GAT?GCC?CTG?AAG?CTT?TCA?TAT?CCA?GAT?GAA?ATA?AAA?GAA???????1680Thr?Leu?Lys?Asp?Ala?Leu?Lys?Leu?Ser?Tyr?Pro?Asp?Glu?Ile?Lys?Glu

1010????????????????1015????????????????1020ATA?GAG?GGA?TTA?TTA?TAT?TAT?AAA?AAC?AAA?CCG?ATA?TAC?GAA?TCG?AGC???????1728Ile?Glu?Gly?Leu?Leu?Tyr?Tyr?Lys?Asn?Lys?Pro?Ile?Tyr?Glu?Ser?Ser

1025????????????????1030????????????????1035GTT?ATG?ACT?TAC?TTA?GAT?GAA?AAT?ACA?GCA?AAA?GAA?GTG?ACC?AAA?CAA???????1776Val?Met?Thr?Tyr?Leu?Asp?Glu?Asn?Thr?Ala?Lys?Glu?Val?Thr?Lys?Gln

1040????????????????1045????????????????1050TTA?AAT?GAT?ACC?ACT?GGG?AAA?TTT?AAA?GAT?GTA?AGT?CAT?TTA?TAT?GAT???????1824Leu?Asn?Asp?Thr?Thr?Gly?Lys?Phe?Lys?Asp?Val?Ser?His?Leu?Tyr?Asp1055????????????????1060????????????????1065????????????????1070GTA?AAA?CTG?ACT?CCA?AAA?ATG?AAT?GTT?ACA?ATC?AAA?TTG?TCT?ATA?CTT???????1872Val?Lys?Leu?Thr?Pro?Lys?Met?Asn?Val?Thr?Ile?Lys?Leu?Ser?Ile?Leu

1075????????????????1080????????????????1085TAT?GAT?AAT?GCT?GAG?TCT?AAT?GAT?AAC?TCA?ATT?GGT?AAA?TGG?ACA?AAC???????1920Tyr?Asp?Asn?Ala?Glu?Ser?Asn?Asp?Asn?Ser?Ile?Gly?Lys?Trp?Thr?Asn

1090????????????????1095????????????????1100ACA?AAT?ATT?GTT?TCA?GGT?GGA?AAT?AAC?GGA?AAA?AAA?CAA?TAT?TCT?TCT???????1968Thr?Asn?Ile?Val?Ser?Gly?Gly?Asn?Asn?Gly?Lys?Lys?Gln?Tyr?Ser?Ser

1105????????????????1110????????????????1115AAT?AAT?CCG?GAT?GCT?AAT?TTG?ACA?TTA?AAT?ACA?GAT?GCT?CAA?GAA?AAA???????2016Asn?Asn?Pro?Asp?Ala?Asn?Leu?Thr?Leu?Asn?Thr?Asp?Ala?Gln?Glu?Lys

1120????????????????1125????????????????1130TTA?AAT?AAA?AAT?CGT?GAC?TAT?TAT?ATA?AGT?TTA?TAT?ATG?AAG?TCA?GAA???????2064Leu?Asn?Lys?Asn?Arg?Asp?Tyr?Tyr?Ile?Ser?Leu?Tyr?Met?Lys?Ser?Glu1135????????????????1140????????????????1145????????????????1150AAA?AAC?ACA?CAA?TGT?GAG?ATT?ACT?ATA?GAT?GGG?GAG?ATT?TAT?CCG?ATC???????2112Lys?Asn?Thr?Gln?Cys?Glu?Ile?Thr?Ile?Asp?Gly?Glu?Ile?Tyr?Pro?Ile

1155????????????????1160????????????????1165ACT?ACA?AAA?ACA?GTG?AAT?GTG?AAT?AAA?GAC?AAT?TAC?AAA?AGA?TTA?GAT???????2160Thr?Thr?Lys?Thr?Val?Asn?Val?Asn?Lys?Asp?Asn?Tyr?Lys?Arg?Leu?Asp

1170????????????????1175????????????????1180ATT?ATA?GCT?CAT?AAT?ATA?AAA?AGT?AAT?CCA?ATT?TCT?TCA?CTT?CAT?ATT???????2208Ile?Ile?Ala?His?Asn?Ile?Lys?Ser?Asn?Pro?Ile?Ser?Ser?Leu?His?Ile

1185????????????????1190????????????????1195AAA?ACG?AAT?GAT?GAA?ATA?ACT?TTA?TTT?TGG?GAT?GAT?ATT?TCT?ATA?ACA???????2256Lys?Thr?Asn?Asp?Glu?Ile?Thr?Leu?Phe?Trp?Asp?Asp?Ile?Ser?Ile?Thr

1200????????????????1205????????????????1210GAT?GTA?GCA?TCA?ATA?AAA?CCG?GAA?AAT?TTA?ACA?GAT?TCA?GAA?ATT?AAA???????2304Asp?Val?Ala?Ser?Ile?Lys?Pro?Glu?Asn?Leu?Thr?Asp?Ser?Glu?Ile?Lys1215????????????????1220????????????????1225????????????????1230CAG?ATT?TAT?AGT?AGG?TAT?GGT?ATT?AAG?TTA?GAA?GAT?GGA?ATC?CTT?ATT???????2352Gln?Ile?Tyr?Ser?Arg?Tyr?Gly?Ile?Lys?Leu?Glu?Asp?Gly?Ile?Leu?Ile

1235????????????????1240????????????????1245GAT?AAA?AAA?GGT?GGG?ATT?CAT?TAT?GGT?GAA?TTT?ATT?AAT?GAA?GCT?AGT??????2400Asp?Lys?Lys?Gly?Gly?Ile?His?Tyr?Gly?Glu?Phe?Ile?Asn?Glu?Ala?Ser

1250????????????????1255????????????????1260TTT?AAT?ATT?GAA?CCA?TTG?CAA?AAT?TAT?GTG?ACC?AAA?TAT?GAA?GTT?ACT???????2448Phe?Asn?Ile?Glu?Pro?Leu?Gln?Asn?Tyr?Val?Thr?Lys?Tyr?Glu?Val?Thr

1265????????????????1270????????????????1275TAT?AGT?AGT?GAG?TTA?GGA?CCA?AAC?GTG?AGT?GAC?ACA?CTT?GAA?AGT?GAT??????2496Tyr?Ser?Ser?Glu?Leu?Gly?Pro?Asn?Val?Ser?Asp?Thr?Leu?Glu?Ser?Asp

1280????????????????1285????????????????1290AAA?ATT?TAC?AAG?GAT?GGG?ACA?ATT?AAA?TIT?GAT?TIT?ACC?AAA?TAT?AGT???????2544Lys?Ile?Tyr?Lys?Asp?Gly?Thr?Ile?Lys?Phe?Asp?Phe?Thr?Lys?Tyr?Ser1295????????????????1300????????????????1305????????????????1310AAA?AAT?GAA?CAA?GGA?TTA?TTT?TAT?GAC?AGT?GGA?TTA?AAT?TGG?GAC?TTT??????2592Lys?Asn?Glu?Gln?Gly?Leu?Phe?Tyr?Asp?Ser?Gly?Leu?Asn?Trp?Asp?Phe

1315????????????????1320????????????????1325AAA?ATT?AAT?GCT?ATT?ACT?TAT?GAT?GGT?AAA?GAG?ATG?AAT?GTT?TTT?CAT??????2640Lys?Ile?Asn?Ala?Ile?Thr?Tyr?Asp?Gly?Lys?Glu?Met?Asn?Val?Phe?His

1330????????????????1335????????????????1340AGA?TAT?AAT?AAA?TAG?????????????????????????????????????????????????????2655Arg?Tyr?Asn?Lys

1345

(2) about the information of SEQ ID NO:5

(ⅰ) sequence signature

(A) length: 884 amino acid

(B) type: amino acid

(D) topological structure: line style

(ⅱ) molecule type: protein (ⅹ ⅰ) sequence description: SEQ ID NO:5:Met Lys Asn Met Lys Lys Lys Leu Ala Ser Val Val Thr Cys Thr Leu 15 10 15Leu Ala Pro Met Phe Leu Asn Gly Asn Val Asn Ala Val Tyr Ala Asp

20??????????????????25??????????????????30Ser?Lys?Thr?Asn?Gln?Ile?Ser?Thr?Thr?Gln?Lys?Asn?Gln?Gln?Lys?Glu

35??????????????????40??????????????????45Met?Asp?Arg?Lys?Gly?Leu?Leu?Gly?Tyr?Tyr?Phe?Lys?Gly?Lys?Asp?Phe

50??????????????????55??????????????????60Ser?Asn?Leu?Thr?Met?Phe?Ala?Pro?Thr?Arg?Asp?Ser?Thr?Leu?Ile?Tyr?65??????????????????70??????????????????75??????????????????80Asp?Gln?Gln?Thr?Ala?Asn?Lys?Leu?Leu?Asp?Lys?Lys?Gln?Gln?Glu?Tyr

85??????????????????90??????????????????95Gln?Ser?Ile?Arg?Trp?Ile?Gly?Leu?Ile?Gln?Ser?Lys?Glu?Thr?Gly?Asp

100?????????????????105?????????????????110Phe?Thr?Phe?Asn?Leu?Ser?Glu?Asp?Glu?Gln?Ala?Ile?Ile?Glu?Ile?Asn

115?????????????????120?????????????????125Gly?Lys?Ile?Ile?Ser?Asn?Lys?Gly?Lys?Glu?Lys?Gln?Val?Val?His?Leu

130?????????????????135?????????????????140Glu?Lys?Gly?Lys?Leu?Val?Pro?Ile?Lys?Ile?Glu?Tyr?Gln?Ser?Asp?Thr145?????????????????150?????????????????155?????????????????160Lys?Phe?Asn?Ile?Asp?Ser?Lys?Thr?Phe?Lys?Glu?Leu?Lys?Leu?Phe?Lys

165?????????????????170?????????????????175Ile?Asp?Ser?Gln?Asn?Gln?Pro?Gln?Gln?Val?Gln?Gln?Asp?Glu?Leu?Arg

180?????????????????185?????????????????190Asn?Pro?Glu?Phe?Asn?Lys?Lys?Glu?Ser?Gln?Glu?Phe?Leu?Ala?Lys?Pro

195?????????????????200?????????????????205Ser?Lys?Ile?Asn?Leu?Phe?Thr?Gln?Lys?Met?Lys?Arg?Glu?Ile?Asp?Glu

210?????????????????215?????????????????220Asp?Thr?Asp?Thr?Asp?Gly?Asp?Ser?Ile?Pro?Asp?Leu?Trp?Glu?Glu?Asn225?????????????????230?????????????????235?????????????????240Gly?Tyr?Thr?Ile?Gln?Asn?Arg?Ile?Ala?Val?Lys?Trp?Asp?Asp?Ser?Leu

245?????????????????250?????????????????255Ala?Ser?Lys?Gly?Tyr?Thr?Lys?Phe?Val?Ser?Asn?Pro?Leu?Glu?Ser?His

260?????????????????265?????????????????270Thr?Val?Gly?Asp?Pro?Tyr?Thr?Asp?Tyr?Glu?Lys?Ala?Ala?Arg?Asp?Leu

275?????????????????280?????????????????285Asp?Leu?Ser?Asn?Ala?Lys?Glu?Thr?Phe?Asn?Pro?Leu?Val?Ala?Ala?Phe

290?????????????????295?????????????????300Pro?Ser?Val?Asn?Val?Ser?Met?Glu?Lys?Val?Ile?Leu?Ser?Pro?Asn?Glu305?????????????????310?????????????????315?????????????????320Asn?Leu?Ser?Asn?Ser?Val?Glu?Ser?His?Ser?Ser?Thr?Asn?Trp?Ser?Tyr

325?????????????????330?????????????????335Thr?Asn?Thr?Glu?Gly?Ala?Ser?Val?Glu?Ala?Gly?Ile?Gly?Pro?Lys?Gly

340?????????????????345?????????????????350Ile?Ser?Phe?Gly?Val?Ser?Val?Asn?Tyr?Gln?His?Ser?Glu?Thr?Val?Ala

355?????????????????360?????????????????365Gln?Glu?Trp?Gly?Thr?Ser?Thr?Gly?Asn?Thr?Ser?Gln?Phe?Asn?Thr?Ala

370?????????????????375?????????????????380Ser?Ala?Gly?Tyr?Leu?Asn?Ala?Asn?Val?Arg?Tyr?Asn?Asn?Val?Gly?Thr385?????????????????390?????????????????395?????????????????400Gly?Ala?Ile?Tyr?Asp?Val?Lys?Pro?Thr?Thr?Ser?Phe?Val?Leu?Asn?Asn

405?????????????????410?????????????????415Asp?Thr?Ile?Ala?Thr?Ile?Thr?Ala?Lys?Ser?Asn?Ser?Thr?Ala?Leu?Asn

420?????????????????425?????????????????430Ile?Ser?Pro?Gly?Glu?Ser?Tyr?Pro?Lys?Lys?Gly?Gln?Asn?Gly?Ile?Ala

435?????????????????440?????????????????445Ile?Thr?Ser?Met?Asp?Asp?Phe?Asn?Ser?His?Pro?Ile?Thr?Leu?Asn?Lys

450?????????????????455?????????????????460Lys?Gln?Val?Asp?Asn?Leu?Leu?Asn?Asn?Lys?Pro?Met?Met?Leu?Glu?Thr465?????????????????470?????????????????475?????????????????480Asn?Gln?Thr?Asp?Gly?Val?Tyr?Lys?Ile?Lys?Asp?Thr?His?Gly?Asn?Ile

485?????????????????490?????????????????495Val?Thr?Gly?Gly?Glu?Trp?Asn?Gly?Val?Ile?Gln?Gln?Ile?Lys?Ala?Lys

500?????????????????505?????????????????510Thr?Ala?Ser?Ile?Ile?Val?Asp?Asp?Gly?Glu?Arg?Val?Ala?Glu?Lys?Arg

515?????????????????520?????????????????525Val?Ala?Ala?Lys?Asp?Tyr?Glu?Asn?Pro?Glu?Asp?Lys?Thr?Pro?Ser?Leu

530?????????????????535?????????????????540Thr?Leu?Lys?Asp?Ala?Leu?Lys?Leu?Ser?Tyr?Pro?Asp?Glu?Ile?Lys?Glu545?????????????????550?????????????????555?????????????????560Ile?Glu?Gly?Leu?Leu?Tyr?Tyr?Lys?Asn?Lys?Pro?Ile?Tyr?Glu?Ser?Ser

565?????????????????570?????????????????575Val?Met?Thr?Tyr?Leu?Asp?Glu?Asn?Thr?Ala?Lys?Glu?Val?Thr?Lys?Gln

580?????????????????585?????????????????590Leu?Asn?Asp?Thr?Thr?Gly?Lys?Phe?Lys?Asp?Val?Ser?His?Leu?Tyr?Asp

595?????????????????600?????????????????605Val?Lys?Leu?Thr?Pro?Lys?Met?Asn?Val?Thr?Ile?Lys?Leu?Ser?Ile?Leu

610?????????????????615?????????????????620Tyr?Asp?Asn?Ala?Glu?Ser?Asn?Asp?Asn?Ser?Ile?Gly?Lys?Trp?Thr?Asn625?????????????????630?????????????????635?????????????????640Thr?Asn?Ile?Val?Ser?Gly?Gly?Asn?Asn?Gly?Lys?Lys?Gln?Tyr?Ser?Ser

645?????????????????650?????????????????655Asn?Asn?Pro?Asp?Ala?Asn?Leu?Thr?Leu?Asn?Thr?Asp?Ala?Gln?Glu?Lys

660?????????????????665?????????????????670Leu?Asn?Lys?Asn?Arg?Asp?Tyr?Tyr?Ile?Ser?Leu?Tyr?Met?Lys?Ser?Glu

675?????????????????680?????????????????685Lys?Asn?Thr?Gln?Cys?Glu?Ile?Thr?Ile?Asp?Gly?Glu?Ile?Tyr?Pro?Ile

690?????????????????695?????????????????700Thr?Thr?Lys?Thr?Val?Asn?Val?Asn?Lys?Asp?Asn?Tyr?Lys?Arg?Leu?Asp705?????????????????710?????????????????715?????????????????720Ile?Ile?Ala?His?Asn?Ile?Lys?Ser?Asn?Pro?Ile?Ser?Ser?Leu?His?Ile

725?????????????????730?????????????????735Lys?Thr?Asn?Asp?Glu?Ile?Thr?Leu?Phe?Trp?Asp?Asp?Ile?Ser?Ile?Thr

740?????????????????745?????????????????750Asp?Val?Ala?Ser?Ile?Lys?Pro?Glu?Asn?Leu?Thr?Asp?Ser?Glu?Ile?Lys

755?????????????????760?????????????????765Gln?Ile?Tyr?Ser?Arg?Tyr?Gly?Ile?Lys?Leu?Glu?Asp?Gly?Ile?Leu?Ile

770?????????????????775?????????????????780Asp?Lys?Lys?Gly?Gly?Ile?His?Tyr?Gly?Glu?Phe?Ile?Asn?Glu?Ala?Ser785?????????????????790?????????????????795?????????????????800Phe?Asn?Ile?Glu?Pro?Leu?Gln?Asn?Tyr?Val?Thr?Lys?Tyr?Glu?Val?Thr

805?????????????????810?????????????????815Tyr?Ser?Ser?Glu?Leu?Gly?Pro?Asn?Val?Ser?Asp?Thr?Leu?Glu?Ser?Asp

820?????????????????825?????????????????830Lys?Ile?Tyr?Lys?Asp?Gly?Thr?Ile?Lys?Phe?Asp?Phe?Thr?Lys?Tyr?Ser

835?????????????????840?????????????????845Lys?Asn?Glu?Gln?Gly?Leu?Phe?Tyr?Asp?Ser?Gly?Leu?Asn?Trp?Asp?Phe

850?????????????????855?????????????????860Lys?Ile?Asn?Ala?Ile?Thr?Tyr?Asp?Gly?Lys?Glu?Met?Asn?Val?Phe?His865?????????????????870?????????????????875?????????????????880Arg?Tyr?Asn?Lys

(2) about the information of SEQ ID NO:6

(ⅰ) sequence signature

(A) length: 2004 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: DNA (genome)

(ⅲ) the false plan: not

(ⅳ) antisense: not

(ⅵ) primary source

(A) biology: Bacillus cercus

(B) bacterial strain: AB78

(C) particular separation thing: NRRL B-21058

(ⅸ) feature:

(A) title/key: CDS

(B) position: 1..2001

(D) out of Memory :/product=" 80kDa VIP1A (a) albumen " ,/remarks=" this sequence is identical with from 3126 to 5126 the nucleotide of SEQ ID NO:1.”

(ⅹ ⅰ) sequence description: SEQ ID NO:6:ATG AAA AGG GAA ATT GAT GAA GAC ACG GAT ACG GAT GGG GAC TCT ATT 48Met Lys Arg Glu Ile Asp Glu Asp Thr Asp Thr Asp Gly Asp Ser Ile885 890 895 900CCT GAC CTT TGG GAA GAA AAT GGG TAT ACG ATT CAA AAT AGA ATC GCT 96Pro Asp Leu Trp Glu Glu Asn Gly Tyr Thr Ile Gln Asn Arg Ile Ala

905?????????????????910?????????????????915GTA?AAG?TGG?GAC?GAT?TCT?CTA?GCA?AGT?AAA?GGG?TAT?ACG?AAA?TTT?GTT??????????144Val?Lys?Trp?Asp?Asp?Ser?Leu?Ala?Ser?Lys?Gly?Tyr?Thr?Lys?Phe?Val

920?????????????????925?????????????????930TCA?AAT?CCA?CTA?GAA?AGT?CAC?ACA?GTT?GGT?GAT?CCP?TAT?ACA?GAT?TAT???????192Ser?Asn?Pro?Leu?Glu?Ser?His?Thr?Val?Gly?Asp?Pro?Tyr?Thr?Asp?Tyr

935?????????????????940?????????????????945GAA?AAG?GCA?GCA?AGA?GAT?CTA?GAT?TTG?TCA?AAT?GCA?AAG?GAA?ACG?TTT???????240Glu?Lys?Ala?Ala?Arg?Asp?Leu?Asp?Leu?Ser?Asn?Ala?Lys?Glu?Thr?Phe

950?????????????????955?????????????????960AAC?CCA?TTG?GTA?GCT?GCT?TTT?CCA?AGT?GTG?AAT?GTT?AGT?ATG?GAA?AAG???????288Asn?Pro?Leu?Val?Ala?Ala?Phe?Pro?Ser?Val?Asn?Val?Ser?Met?Glu?Lys965?????????????????970?????????????????975?????????????????980GTG?ATA?TTA?TCA?CCA?AAT?GAA?AAT?TTA?TCC?AAT?AGT?GTA?GAG?TCT?CAT???????336Val?Ile?Leu?Ser?Pro?Asn?Glu?Asn?Leu?Ser?Asn?Ser?Val?Glu?Ser?His

985?????????????????990?????????????????995TCA?TCC?ACG?AAT?TGG?TCT?TAT?ACA?AAT?ACA?GAA?GGT?GCT?TCT?GTT?GAA???????384Ser?Ser?Thr?Asn?Trp?Ser?Tyr?Thr?Asn?Thr?Glu?Gly?Ala?Ser?Val?Glu

1000????????????????1005????????????????1010GCG?GGG?ATT?GGA?CCA?AAA?GGT?ATT?TCG?TIC?GGA?GTT?AGC?GTA?AAC?TAT???????432Ala?Gly?Ile?Gly?Pro?Lys?Gly?Ile?Ser?Phe?Gly?Val?Ser?Val?Asn?Tyr

1015????????????????1020????????????????1025CAA?CAC?TCT?GAA?ACA?GTT?GCA?CAA?GAA?TGG?GGA?ACA?TCT?ACA?GGA?AAT???????480Gln?His?Ser?Glu?Thr?Val?Ala?Gln?Glu?Trp?Gly?Thr?Ser?Thr?Gly?Asn

1030????????????????1035?????????????????1040ACT?TCG?CAA?TIC?AAT?ACG?GCT?TCA?GCG?GGA?TAT?TTA?AAT?GCA?AAT?GTT???????528Thr?Ser?Gln?Phe?Asn?Thr?Ala?Ser?Ala?Gly?Tyr?Leu?Asn?Ala?Asn?Val1045????????????????1050????????????????1055????????????????1060CGA?TAT?AAC?AAT?TTA?GGA?ACT?GGT?GCC?ATC?TAC?GAT?GTA?AAA?CCT?ACA???????576Arg?Tyr?Asn?Asn?Val?Gly?Thr?Gly?Ala?Ile?Tyr?Asp?Val?Lys?Pro?Thr

1065????????????????1070????????????????1075ACA?AGT?TTT?GTA?TTA?AAT?AAC?GAT?ACT?ATC?GCA?ACT?ATT?ACG?GCG?AAA???????624Thr?Ser?Phe?Val?Leu?Asn?Asn?Asp?Thr?Ile?Ala?Thr?Ile?Thr?Ala?Lys

1080????????????????1085????????????????1090TCT?AAT?TCT?ACA?GCC?TTA?AAT?ATA?TCT?CCT?GGA?GAA?AGT?TAC?CCG?AAA???????672Ser?Asn?Ser?Thr?Ala?Leu?Asn?Ile?Ser?Pro?Gly?Glu?Ser?Tyr?Pro?Lys

1095????????????????1100????????????????1105AAA?GGA?CAA?AAT?GGA?ATC?GCA?ATA?ACA?TCA?ATG?GAT?GAT?TIT?AAT?TCC???????720Lys?Gly?Gln?Asn?Gly?Ile?Ala?Ile?Thr?Ser?Met?Asp?Asp?Phe?Asn?Ser

1110????????????????1115????????????????1120CAT?CCG?ATT?ACA?TPA?AAT?AAA?AAA?CAA?GTA?GAT?AAT?CTG?CTA?AAT?AAT???????768His?Pro?Ile?Thr?Leu?Asn?Lys?Lys?Gln?Val?Asp?Asn?Leu?Leu?Asn?Asn1125????????????????1130????????????????1135????????????????1140AAA?CCT?ATG?ATG?TTG?GAA?ACA?AAC?CAA?ACA?GAT?GGT?GTT?TAT?AAG?ATA???????816Lys?Pro?Met?Met?Leu?Glu?Thr?Asn?Gln?Thr?Asp?Gly?Val?Tyr?Lys?Ile

1145????????????????1150????????????????1155AAA?GAT?ACA?CAT?GGA?AAT?ATA?GTA?ACT?GGC?GGA?GAA?TGG?AAT?GGT?GTC???????864Lys?Asp?Thr?His?Gly?Asn?Ile?Val?Thr?Gly?Gly?Glu?Trp?Asn?Gly?ValGAA?AAG?GCA?GCA?AGA?GAT?CTA?GAT?TTG?TCA?AAT?GCA?AAG?GAA?ACG?TIT?????????240Glu?Lys?Ala?Ala?Arg?Asp?Leu?Asp?Leu?Ser?Asn?Ala?Lys?Glu?Thr?Phe

950?????????????????955?????????????????960AAC?CCA?TTG?GTA?GCT?CCT?TTT?CCA?AGT?GTG?AAT?GTT?AGT?ATG?GAA?AAG?????????288Asn?Pro?Leu?Val?Ala?Ala?Phe?Pro?Ser?Val?Asn?Val?Ser?Met?Glu?Lys965?????????????????970?????????????????975?????????????????980GTG?ATA?TTA?TCA?CCA?AAT?GAA?AAT?TTA?TCC?AAT?AGT?GTA?GAG?TCT?CAT?????????336Val?Ile?Leu?Ser?Pro?Asn?Glu?Asn?Leu?Ser?Asn?Ser?Val?Glu?Ser?His

985?????????????????990?????????????????995TCA?TCC?ACG?AAT?TGG?TCT?TAT?ACA?AAT?ACA?GAA?GGT?GCT?TCT?GTT?GAA?????????384Ser?Ser?Thr?Asn?Trp?Ser?Tyr?Thr?Asn?Thr?Glu?Gly?Ala?Ser?Val?Glu

1000????????????????1005????????????????1010GCG?GGG?ATT?GGA?CCA?AAA?GGT?ATT?TCG?TTC?GGA?GTT?AGC?GTA?AAC?TAT?????????432Ala?Gly?Ile?Gly?Pro?Lys?Gly?Ile?Ser?Phe?Gly?Val?Ser?Val?Asn?Tyr

1015????????????????1020????????????????1025CAA?CAC?TCT?GAA?ACA?GTT?GCA?CAA?GAA?TGG?GGA?ACA?TCT?ACA?GGA?AAT?????????480Gln?His?Ser?Glu?Thr?Val?Ala?Gln?Glu?Trp?Gly?Thr?Ser?Thr?Gly?Asn

1030????????????????1035????????????????1040

ACT?TCG?CAA?TTC?AAT?ACG?GCT?TCA?GCG?GGA?TAT?TTA?AAT?GCA?AAT?GTT?????????528Thr?Ser?Gln?Phe?Asn?Thr?Ala?Ser?Ala?Gly?Tyr?Leu?Asn?Ala?Asn?Val1045????????????????1050????????????????1055????????????????1060CGA?TAT?AAC?AAT?GTA?GGA?ACT?GGT?GCC?ATC?TAC?GAT?GTA?AAA?CCT?ACA?????????576Arg?Tyr?Asn?Asn?Val?Gly?Thr?Gly?Ala?Ile?Tyr?Asp?Val?Lys?Pro?Thr

1065????????????????1070????????????????1075ACA?AGT?TTT?GTA?TTA?AAT?AAC?GAT?ACT?ATC?GCA?ACT?ATT?ACG?GCG?AAA?????????624Thr?Ser?Phe?Val?Leu?Asn?Asn?Asp?Thr?Ile?Ala?Thr?Ile?Thr?Ala?Lys

1080????????????????1085????????????????1090TCT?AAT?TCT?ACA?GCC?TTA?AAT?ATA?TCT?CCT?GGA?GAA?AGT?TAC?CCG?AAA?????????672Ser?Asn?Ser?Thr?Ala?Leu?Asn?Ile?Ser?Pro?Gly?Glu?Ser?Tyr?Pro?Lys

1095????????????????1100????????????????1105AAA?GGA?CAA?AAT?GGA?ATC?GCA?ATA?ACA?TCA?ATG?GAT?GAT?TTT?AAT?TCC?????????720Lys?Gly?Gln?Asn?Gly?Ile?Ala?Ile?Thr?Ser?Met?Asp?Asp?Phe?Asn?Ser

1110????????????????1115????????????????1120CAT?CCG?ATT?ACA?TTA?AAT?AAA?AAA?CAA?GTA?GAT?AAT?CTG?CTA?AAT?AAT?????????768His?Pro?Ile?Thr?Leu?Asn?Lys?Lys?Gln?Val?Asp?Asn?Leu?Leu?Asn?Asn1125????????????????1130????????????????1135????????????????1140AAA?CCT?ATG?ATG?TTG?GAA?ACA?AAC?CAA?ACA?GAT?GGT?GTT?TAT?AAG?ATA?????????816Lys?Pro?Met?Met?Leu?Glu?Thr?Asn?Gln?Thr?Asp?Gly?Val?Tyr?Lys?Ile

1145????????????????1150????????????????1155AAA?GAT?ACA?CAT?GGA?AAT?ATA?GTA?ACT?GGC?GGA?GAA?TGG?AAT?GGT?GTC?????????864Lys?Asp?Thr?His?Gly?Asn?Ile?Val?Thr?Gly?Gly?Glu?Trp?Asn?Gly?Val

1160????????????????1165????????????????1170ATA?CAA?CAA?ATC?AAG?GCT?AAA?ACA?GCG?TCT?ATT?ATT?GTG?GAT?GAT?GGG???????912Ile?Gln?Gln?Ile?Lys?Ala?Lys?Thr?Ala?Ser?Ile?Ile?Val?Asp?Asp?Gly

1175????????????????1180????????????????1185GAA?CGT?GTA?GCA?GAA?AAA?CGT?GTA?GCG?GCA?AAA?GAT?TAT?GAA?AAT?CCA???????960Glu?Arg?Val?Ala?Glu?Lys?Arg?Val?Ala?Ala?Lys?Asp?Tyr?Glu?Asn?Pro

1190????????????????1195????????????????1200GAA?GAT?AAA?ACA?CCG?TCT?TTA?ACT?TTA?AAA?GAT?GCC?CTG?AAG?CTT?TCA??????1008Glu?Asp?Lys?Thr?Pro?Ser?Leu?Thr?Leu?Lys?Asp?Ala?Leu?Lys?Leu?Ser1205????????????????1210????????????????1215????????????????1220TAT?CCA?GAT?GAA?ATA?AAA?GAA?ATA?GAG?GGA?TTA?TTA?TAT?TAT?AAA?AAC??????1056Tyr?Pro?Asp?Glu?Ile?Lys?Glu?Ile?Glu?Gly?Leu?Leu?Tyr?Tyr?Lys?Asn

1225????????????????1230????????????????1235AAA?CCG?ATA?TAC?GAA?TCG?AGC?GTT?ATG?ACT?TAC?TTA?GAT?GAA?AAT?ACA??????1104Lys?Pro?Ile?Tyr?Glu?Ser?Ser?Val?Met?Thr?Tyr?Leu?Asp?Glu?Asn?Thr

1240????????????????1245????????????????1250GCA?AAA?GAA?GTG?ACC?AAA?CAA?TTA?AAT?GAT?ACC?ACT?GGG?AAA?TTT?AAA??????1152Ala?Lys?Glu?Val?Thr?Lys?Gln?Leu?Asn?Asp?Thr?Thr?Gly?Lys?Phe?Lys

1255????????????????1260????????????????1265GAT?GTA?AGT?CAT?TTA?TAT?GAT?GTA?AAA?CTG?ACT?CCA?AAA?ATG?AAT?GTT??????1200Asp?Val?Ser?His?Leu?Tyr?Asp?Val?Lys?Leu?Thr?Pro?Lys?Met?Asn?Val

1270????????????????1275????????????????1280ACA?ATC?AAA?TTG?TCT?ATA?CTT?TAT?GAT?AAT?GCT?GAG?TCT?AAT?GAT?AAC??????1248Thr?Ile?Lys?Leu?Ser?Ile?Leu?Tyr?Asp?Asn?Ala?Glu?Ser?Asn?Asp?Asn1285????????????????1290????????????????1295????????????????1300TCA?ATT?GGT?AAA?TGG?ACA?AAC?ACA?AAT?ATT?GTT?TCA?GGT?GGA?AAT?AAC??????1296Ser?Ile?Gly?Lys?Trp?Thr?Asn?Thr?Asn?Ile?Val?Ser?Gly?Gly?Asn?Asn

1305????????????????1310????????????????1315GGA?AAA?AAA?CAA?TAT?TCT?TCT?AAT?AAT?CCG?GAT?GCT?AAT?TTG?ACA?TTA??????1344Gly?Lys?Lys?Gln?Tyr?Ser?Ser?Asn?Asn?Pro?Asp?Ala?Asn?Leu?Thr?Leu

1320????????????????1325????????????????1330AAT?ACA?GAT?GCT?CAA?GAA?AAA?TTA?AAT?AAA?AAT?CGT?GAC?TAT?TAT?ATA??????1392Asn?Thr?Asp?Ala?Gln?Glu?Lys?Leu?Asn?Lys?Asn?Arg?Asp?Tyr?Tyr?Ile

1335????????????????1340????????????????1345AGT?TTA?TAT?ATG?AAG?TCA?GAA?AAA?AAC?ACA?CAA?TGT?GAG?ATT?ACT?ATA??????1440Ser?Leu?Tyr?Met?Lys?Ser?Glu?Lys?Asn?Thr?Gln?Cys?Glu?Ile?Thr?Ile

1350????????????????1355????????????????1360GAT?GGG?GAG?ATT?TAT?CCG?ATC?ACT?ACA?AAA?ACA?GTG?AAT?GTG?AAT?AAA??????1488Asp?Gly?Glu?Ile?Tyr?Pro?Ile?Thr?Thr?Lys?Thr?Val?Asn?Val?Asn?Lys1365????????????????1370????????????????1375????????????????1380GAC?AAT?TAC?AAA?AGA?TTA?GAT?ATT?ATA?GCT?CAT?AAT?ATA?AAA?AGT?AAT??????1536Asp?Asn?Tyr?Lys?Arg?Leu?Asp?Ile?Ile?Ala?His?Asn?Ile?Lys?Ser?Asn

1385????????????????1390????????????????1395CCA?ATT?TCT?TCA?CTT?CAT?ATT?AAA?ACG?AAT?GAT?GAA?ATA?ACT?TTA?TTT???????1584Pro?Ile?Ser?Ser?Leu?His?Ile?Lys?Thr?Asn?Asp?Glu?Ile?Thr?Leu?Phe

1400????????????????1405????????????????1410TGG?GAT?GAT?ATT?TCT?ATA?ACA?GAT?GTA?GCA?TCA?ATA?AAA?CCG?GAA?AAT???????1632Trp?Asp?Asp?Ile?Ser?Ile?Thr?Asp?Val?Ala?Ser?Ile?Lys?Pro?Glu?Asn

1415????????????????1420????????????????1425TTA?ACA?GAT?TCA?GAA?ATT?AAA?CAG?ATT?TAT?AGT?AGG?TAT?GGT?ATT?AAG???????1680Leu?Thr?Asp?Ser?Glu?Ile?Lys?Gln?Ile?Tyr?Ser?Arg?Tyr?Gly?Ile?Lys

1430????????????????1435????????????????1440TTA?GAA?GAT?GGA?ATC?CTT?ATT?GAT?AAA?AAA?GGT?GGG?ATT?CAT?TAT?GGT???????1728Leu?Glu?Asp?Gly?Ile?Leu?Ile?Asp?Lys?Lys?Gly?Gly?Ile?His?Tyr?Gly1445????????????????1450????????????????1455????????????????1460GAA?TTT?ATT?AAT?GAA?GCT?AGT?TTT?AAT?ATT?GAA?CCA?TTG?CCA?AAT?TAT???????1776Glu?Phe?Ile?Asn?Glu?Ala?Ser?Phe?Asn?Ile?Glu?Pro?Leu?Pro?Asn?Tyr

1465????????????????1470????????????????1475GTG?ACC?AAA?TAT?GAA?GTT?ACT?TAT?AGT?AGT?GAG?TTA?GGA?CCA?AAC?GTG???????1824Val?Thr?Lys?Tyr?Glu?Val?Thr?Tyr?Ser?Ser?Glu?Leu?Gly?Pro?Asn?Val

1480????????????????1485????????????????1490AGT?GAC?ACA?CTT?GAA?AGT?GAT?AAA?ATT?TAC?AAG?GAT?GGG?ACA?ATT?AAA???????1872Ser?Asp?Thr?Leu?Glu?Ser?Asp?Lys?Ile?Tyr?Lys?Asp?Gly?Thr?Ile?Lys

1495????????????????1500????????????????1505TTT?GAT?TTT?ACC?AAA?TAT?AGT?AAA?AAT?GAA?CAA?GGA?TTA?TTT?TAT?GAC???????1920Phe?Asp?Phe?Thr?Lys?Tyr?Ser?Lys?Asn?Glu?Gln?Gly?Leu?Phe?Tyr?Asp

1510????????????????1515????????????????1520AGT?GGA?TTA?AAT?TGG?GAC?TTT?AAA?ATT?AAT?GCT?ATT?ACT?TAT?GAT?GGT???????1968Ser?Gly?Leu?Asn?Trp?Asp?Phe?Lys?Ile?Asn?Ala?Ile?Thr?Tyr?Asp?Gly1525????????????????1530????????????????1535????????????????1540AAA?GAG?ATG?AAT?GTT?TTT?CAT?AGA?TAT?AAT?AAA?TAG???????????????????????2004Lys?Glu?Met?Asn?Val?Phe?His?Arg?Tyr?Asn?Lys

1545????????????????1550

(2) about the information of SEQ ID NO:7

(ⅰ) sequence signature

(A) length: 667 amino acid

(B) type: amino acid

(D) topological structure: line style

(ⅱ) molecule type: protein

(ⅹ ⅰ) sequence description: SEQ ID NO:7:Met Lys Arg Glu Ile Asp Glu Asp Thr Asp Thr Asp Gly Asp Ser Ile 15 10 15Pro Asp Leu Trp Glu Glu Asn Gly Tyr Thr Ile Gln Asn Arg Ile Ala

20??????????????????25??????????????????30Val?Lys?Trp?Asp?Asp?Ser?Leu?Ala?Ser?Lys?Gly?Tyr?Thr?Lys?Phe?Val

35??????????????????40??????????????????45Ser?Asn?Pro?Leu?Glu?Ser?His?Thr?Val?Gly?Asp?Pro?Tyr?Thr?Asp?Tyr

50??????????????????55??????????????????60Glu?Lys?Ala?Ala?Arg?Asp?Leu?Asp?Leu?Ser?Asn?Ala?Lys?Glu?Thr?Phe?65??????????????????70??????????????????75??????????????????80Asn?Pro?Leu?Val?Ala?Ala?Phe?Pro?Ser?Val?Asn?Val?Ser?Met?Glu?Lys

85??????????????????90??????????????????95Val?Ile?Leu?Ser?Pro?Asn?Glu?Asn?Leu?Ser?Asn?Ser?Val?Glu?Ser?His

100?????????????????105?????????????????110Ser?Ser?Thr?Asn?Trp?Ser?Tyr?Thr?Asn?Thr?Glu?Gly?Ala?Ser?Val?Glu

115?????????????????120?????????????????125Ala?Gly?Ile?Gly?Pro?Lys?Gly?Ile?Ser?Phe?Gly?Val?Ser?Val?Asn?Tyr

130?????????????????135?????????????????140Gln?His?Ser?Glu?Thr?Val?Ala?Gln?Glu?Trp?Gly?Thr?Ser?Thr?Gly?Asn145?????????????????150?????????????????155?????????????????160Thr?Ser?Gln?Phe?Asn?Thr?Ala?Ser?Ala?Gly?Tyr?Leu?Asn?Ala?Asn?Val

165?????????????????170?????????????????175Arg?Tyr?Asn?Asn?Val?Gly?Thr?Gly?Ala?Ile?Tyr?Asp?Val?Lys?Pro?Thr

180?????????????????185?????????????????190Thr?Ser?Phe?Val?Leu?Asn?Asn?Asp?Thr?Ile?Ala?Thr?Ile?Thr?Ala?Lys

195?????????????????200?????????????????205Ser?Asn?Ser?Thr?Ala?Leu?Asn?Ile?Ser?Pro?Gly?Glu?Ser?Tyr?Pro?Lys

210?????????????????215?????????????????220Lys?Gly?Gln?Asn?Gly?Ile?Ala?Ile?Thr?Ser?Met?Asp?Asp?Phe?Asn?Ser225?????????????????230?????????????????235?????????????????240His?Pro?Ile?Thr?Leu?Asn?Lys?Lys?Gln?Val?Asp?Asn?Leu?Leu?Asn?Asn

245?????????????????250?????????????????255Lys?Pro?Met?Met?Leu?Glu?Thr?Asn?Gln?Thr?Asp?Gly?Val?Tyr?Lys?Ile

260?????????????????265?????????????????270Lys?Asp?Thr?His?Gly?Asn?Ile?Val?Thr?Gly?Gly?Glu?Trp?Asn?Gly?Val

275?????????????????280?????????????????285Ile?Gln?Gln?Ile?Lys?Ala?Lys?Thr?Ala?Ser?Ile?Ile?Val?Asp?Asp?Gly

290?????????????????295?????????????????300Glu?Arg?Val?Ala?Glu?Lys?Arg?Val?Ala?Ala?Lys?Asp?Tyr?Glu?Asn?Pro305?????????????????310?????????????????315?????????????????320Glu?Asp?Lys?Thr?Pro?Ser?Leu?Thr?Leu?Lys?Asp?Ala?Leu?Lys?Leu?Ser

325?????????????????330?????????????????335Tyr?Pro?Asp?Glu?Ile?Lys?Glu?Ile?Glu?Gly?Leu?Leu?Tyr?Tyr?Lys?Asn

340?????????????????345?????????????????350Lys?Pro?Ile?Tyr?Glu?Ser?Ser?Val?Met?Thr?Tyr?Leu?Asp?Glu?Asn?Thr

355?????????????????360?????????????????365Ala?Lys?Glu?Val?Thr?Lys?Gln?Leu?Asn?Asp?Thr?Thr?Gly?Lys?Phe?Lys

370?????????????????375?????????????????380Asp?Val?Ser?His?Leu?Tyr?Asp?Val?Lys?Leu?Thr?Pro?Lys?Met?Asn?Val385?????????????????390?????????????????395?????????????????400Thr?Ile?Lys?Leu?Ser?Ile?Leu?Tyr?Asp?Asn?Ala?Glu?Ser?Asn?Asp?Asn

405?????????????????410?????????????????415Ser?Ile?Gly?Lys?Trp?Thr?Asn?Thr?Asn?Ile?Val?Ser?Gly?Gly?Asn?Asn

420?????????????????425?????????????????430Gly?Lys?Lys?Gln?Tyr?Ser?Ser?Asn?Asn?Pro?Asp?Ala?Asn?Leu?Thr?Leu

435?????????????????440?????????????????445Asn?Thr?Asp?Ala?Gln?Glu?Lys?Leu?Asn?Lys?Asn?Arg?Asp?Tyr?Tyr?Ile

450?????????????????455?????????????????460Ser?Leu?Tyr?Met?Lys?Ser?Glu?Lys?Asn?Thr?Gln?Cys?Glu?Ile?Thr?Ile465?????????????????470?????????????????475?????????????????480Asp?Gly?Glu?Ile?Tyr?Pro?Ile?Thr?Thr?Lys?Thr?Val?Asn?Val?Asn?Lys

485?????????????????490?????????????????495Asp?Asn?Tyr?Lys?Arg?Leu?Asp?Ile?Ile?Ala?His?Asn?Ile?Lys?Ser?Asn

500?????????????????505?????????????????510Pro?Ile?Ser?Ser?Leu?His?Ile?Lys?Thr?Asn?Asp?Glu?Ile?Thr?Leu?Phe

515?????????????????520?????????????????525Trp?Asp?Asp?Ile?Ser?Ile?Thr?Asp?Val?Ala?Ser?Ile?Lys?Pro?Glu?Asn

530?????????????????535?????????????????540Leu?Thr?Asp?Ser?Glu?Ile?Lys?Gln?Ile?Tyr?Ser?Arg?Tyr?Gly?Ile?Lys545?????????????????550?????????????????555?????????????????560Leu?Glu?Asp?Gly?Ile?Leu?Ile?Asp?Lys?Lys?Gly?Gly?Ile?His?Tyr?Gly

565?????????????????570?????????????????575Glu?Phe?Ile?Asn?Glu?Ala?Ser?Phe?Asn?Ile?Glu?Pro?Leu?Pro?Asn?Tyr

580?????????????????585?????????????????590Val?Thr?Lys?Tyr?Glu?Val?Thr?Tyr?Ser?Ser?Glu?Leu?Gly?Pro?Asn?Val

595?????????????????600?????????????????605Ser?Asp?Thr?Leu?Glu?Ser?Asp?Lys?Ile?Tyr?Lys?Asp?Gly?Thr?Ile?Lys

610?????????????????615?????????????????620Phe?Asp?Phe?Thr?Lys?Tyr?Ser?Lys?Asn?Glu?Gln?Gly?Leu?Phe?Tyr?Asp625?????????????????630?????????????????635?????????????????640Ser?Gly?Leu?Asn?Trp?Asp?Phe?Lys?Ile?Asn?Ala?Ile?Thr?Tyr?Asp?Gly

645?????????????????650?????????????????655Lys?Glu?Met?Asn?Val?Phe?His?Arg?Tyr?Asn?Lys

660?????????????????665

(2) about the information of SEQ ID NO:8

(ⅰ) sequence signature

(A) length: 16 amino acid

(B) type: amino acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: peptide

(ⅲ) the false plan: not

(ⅴ) clip types: N-end

(ⅵ) primary source

(A) biology: Bacillus cercus

(B) bacterial strain: AB78

(C) particular separation thing: NRRL B-21058

(ⅸ) feature:

(A) title/key: peptide

(B) position: 1..16

(D) out of Memory :/remarks=from the N terminal sequence of the albumen of AB18 bacterial strain purifying

(ⅹ ⅰ) sequence description: SEQ ID NO:8:Lys Arg Glu Ile Asp Glu Asp Thr Asp Thr Asx Gly Asp Ser Ile Pro1 5 10 15

(2) about the information of SEQ ID NO:9

(ⅰ) sequence signature

(A) length: 21 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: DNA (genome)

(ⅲ) the false plan: not

(ⅳ) antisense: not

(ⅸ) feature:

(A) title/key: misc_feature

(B) position: 1..21

(D) out of Memory :/remarks=", use the codon usage of bacillus thuringiensis based on the oligonucleotide probe of 3 to 9 amino acids of SEQ ID NO:8.”

(ⅹ ⅰ) sequence description: SEQ ID NO:9:GAAATIGATC AAGATACNGAT 21

(2) about the information of SEQ ID NO:10

(ⅰ) sequence signature

(A) length: 14 amino acid

(B) type: amino acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: protein

(ⅲ) the false plan: not

(ⅴ) clip types: N-end

(ⅵ) primary source

(A) biology: bacillus thuringiensis

(B) bacterial strain: AB88 (ⅸ) feature:

(A) title/key: peptide

(B) position: 1..14

(D) out of Memory :/remarks=" the N terminal amino acid sequence (littler) that is called the albumen of anion exchange component 23.”

(ⅹ ⅰ) sequence description: SEQ ID NO:10:Xaa Glu Pro Phe Val Ser Ala Xaa Xaa Xaa Gln Xaa Xaa Xaa1 5 10

(2) about the information of SEQ ID NO:11

(ⅱ) sequence signature

(A) length: 13 amino acid

(B) type: amino acid

(C) chain: strand

(D) topological structure: N-end

(ⅵ) primary source

(A) biology: bacillus thuringiensis

(ⅹ ⅰ) sequence description: SEQ ID NO:11:Xaa Glu Tyr Glu Asn Val Glu Pro Phe Val Ser Ala Xaa1 5 10

(2) about the information of SEQ ID NO:12

(ⅰ) sequence signature

(A) length: 14 amino acid

(B) type: amino acid

(C) chain: strand

(D) topological structure: N-end

(ⅵ) primary source

(A) biology: bacillus thuringiensis

(ⅹ ⅰ) sequence description: SEQ ID NO:12:Met Asn Lys Asn Asn Thr Lys Leu Pro Thr Arg Ala Leu Pro1 5 10

(2) about the information of SEQ ID NO:13

(ⅰ) sequence signature

(A) length: 15 amino acid

(B) type: amino acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: protein

(ⅲ) the false plan: not

(ⅴ) clip types: N-end

(ⅵ) primary source

(A) biology: bacillus thuringiensis

(B) bacterial strain: AB88

(ⅸ) feature:

(A) title/key: peptide

(B) position: 1..15

(D) out of Memory :/remarks=" to the N terminal amino acid sequence of the activated 35kDa VIP of Agrotis ipsilon.”

(ⅹ ⅰ) sequence description: SEQ ID NO:13:Ala Leu Ser Glu Asn Thr Gly Lys Asp Gly Gly Tyr Ile Val Pro1 5 10 15

(2) about the information of SEQ ID NO:14

(ⅰ) sequence signature

(A) length: 9 amino acid

(B) type: amino acid

(C) chain: strand

(D) topological structure: N-end

(ⅵ) primary source

(A) biology: bacillus thuringiensis

(ⅹ ⅰ) sequence description: SEQ ID NO:14:

Met?Asp?Asn?Asn?Pro?Asn?Ile?Asn?Glu

1???????????????5

(2) about the information of SEQ ID NO:15

(ⅰ) sequence signature

(A) length: 9 amino acid

(B) type: amino acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: protein

(ⅲ) the false plan: not

(ⅴ) clip types: N-end

(ⅸ) feature:

(A) title/key: peptide

(B) position: 1..9

(D) out of Memory :/remarks=" the N terminal sequence of 80kDa delta-endotoxin.”

(ⅹ ⅰ) sequence description: SEQ ID NO:15:

Met?Asp?Asn?Asn?Pro?Asn?Ile?Asn?Glu

1???????????????5

(2) about the information of SEQ ID NO:16

(ⅰ) sequence signature

(A) length: 11 amino acid

(B) type: amino acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: peptide

(ⅲ) the false plan: not

(ⅴ) clip types: N-end

(ⅵ) primary source

(A) biology: bacillus thuringiensis

(ⅸ) feature:

(A) title/key: peptide

(B) position: 1.11

(D) out of Memory :/remarks=" the N terminal sequence of 60kDa delta-endotoxin.”

(ⅹ ⅰ) sequence description: SEQ ID NO:16:

Met?Asn?Val?Leu?Asn?Ser?Gly?Arg?Thr?Thr?Ile

1???????????????5???????????????????10

(2) about the information of SEQ ID NO:17

(ⅰ) sequence signature

(A) length: 2655 nucleotide

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: DNA (genome)

(ⅲ) the false plan: not

(ⅳ) antisense: not

(ⅸ) feature:

(A) title/key: misc_feathure

(B) position: 1.2652

(D) out of Memory :/remarks=" from the corn optimization dna sequence dna of coding 100kd VIP1A (a) albumen of AB78.”

( ⅹⅰ ) ：SEQ ID NO:17:ATGAAGAACA TGAAGAAGAA GCTGGCCAGC GTGGGTGACCT GCACCCTGCT GGCCCCCATG 60TTCCTGAACG GCAACGTGAA CGCCGTGTAC GCCGACAGCA AGACCAACCA GATCAGCACC 120ACCCAGAAGA ACCAGCAGAA GGAGATGGAC CGCAAGGGCC TGCTGGGCTA CTACTTCAAG 180GGCAAGGACT TCAGCAACCT GACCATGTTC GCCCCCACGC GTGACAGCAC CCTGATCTAC 240GACCAGCAGA CCGCCAACAA GCTGCTGGAC AAGAAGCAGC AGGAGTACCA GAGCATCCGC 300TGGATCGGCC TGATCCAGAG CAAGGAGACC GGCGACTTCA CCTTCAACCT GAGCGAGGAC 360GAGCAGGCCA TCATCGAGAT CAACGGCAAG ATCATCAGCA ACAAGGGCAA GGAGAAGCAG 420GTGGTGCACC TGGAGAAGGG CAAGCTGGTG CCCATCAAGA TCGAGTACCA GAGCGACACC 480AAGTTCAACA TCGACAGCAA GACCTTCAAG GAGCTGAAGC TTTTCAAGAT CGACAGCCAG 540AACCAGCCCC AGCAGGTGCA GCAGGACGAG CTGCGCAACC CCGAGTTCAA CAAGAAGGAG 600AGCCAGGAGT TCCTGGCCAA GCCCAGCAAG ATCAACCTGT TCACCCAGCA GATGAAGCGC 660GAGATCGACG AGGACACCGA CACCGACGGC GACAGCATCC CCGACCTGTG GGAGGAGAAC 720GGCTACACCA TCCAGAACCG CATCGCCGTG AAGTGGGACG ACAGCCTGGC TAGCAAGGGC 780TACACCAAGT TCGTGAGCAA CCCCCTGGAG AGCCACACCG TGGGCGACCC CTACACCGAC 840TACGAGAAGG CCGCCCGCGA CCTGGACCTG AGCAACGCCA AGGAGACCTT CAACCCCCTG 900GTGGCCGCCT TCCCCAGCGT GAACGTGAGC ATGGAGAAGG TGATCCTGAG CCCCAACGAG 960AACCTGAGCA ACAGCGTGGA GAGCCACTCG AGCACCAACT GGAGCTACAC CAACACCGAG 1020GGCGCCAGCG TGGAGGCCGG CATCGGTCCC AAGGGCATCA GCTTCGGCGT GAGCGTGAAC 1080TACCAGCACA GCGAGACCGT GGCCCAGGAG TGGGGCACCA GCACCGGCAA CACCAGCCAG 1140TTCAACACCG CCAGCGCCGG CTACCTGAAC GCCAACGTGC GCTACAACAA CGTGGGCACC 1200GGCGCCATCT ACGACGTGAA GCCCACCACC AGCTTCGTGC TGAACAACGA CACCATCGCC 1260ACCATCACCG CCAAGTCGAA TTCCACCGCC CTGAACATCA GCCCCGGCGA GAGCTACCCC 1320AAGAAGGGCC AGAACGGCAT CGCCATCACC AGCATGGACG ACTTCAACAG CCACCCCATC 1380ACCCTGAACA AGAAGCAGGT GGACAACCTG CTGAACAACA AGCCCATGAT GCTGGAGACC 1440AACCAGACCG ACGGCGTCTA CAACATCAAG GACACCCACG GCAACATCGT GACCGGCGGC 1500GAGTGGAACG GCGTGATCCA GCAGATCAAG GCCAAGACCG CCAGCATCAT CGTCGACGAC 1560GGCGAGCGCG TGGCCGAGAA GCGCGTGGCC GCCAAGGACT ACGAGAACCC CGAGGACAAG 1620ACCCCCAGCC TGACCCTGAA GGACGCCCTG AAGCTGAGCT ACCCCGACGA GATCAAGGAG 1680ATCGAGGGCC TGCTGTACTA CAAGAACAAG CCCATCTACG AGAGCAGCGT GATGACCTAT 1740CTAGACGAGA ACACCGCCAA GGAGGTGACC AAGCAGCTGA ACGACACCAC CGGCAAGTTC 1800AAGGACGTGA GCCACCTGTA CGACGTGAAG CTGACCCCCA AGATGAACGT GACCATCAAG 1860CTGAGCATCC TGTACGACAA CGCCGAGAGC AACGACAACA GCATCGGCAA GTGGACCAAC 1920ACCAACATCG TGAGCGGCGG CAACAACGGC AAGAAGCAGT ACAGCAGCAA CAACCCCGAC 1980GCCAACCTGA CCCTGAACAC CGACGCCCAG GAGAAGCTGA ACAAGAACCG CGACTACTAC 2040ATCAGCCTGT ACATGAAGAG CGAGAAGAAC ACCCAGTGCG AGATCACCAT CGACGGCGAG 2100ATATACCCCA TCACCACCAA GACCGTGAAC GTGAACAAGG ACAACTACAA GCGCCTGGAC 2160ATCATCGCCC ACAACATCAA GAGCAACCCC ATCAGCAGCC TGCACATCAA GACCAACGAC 2220GAGATCACCC TGTTCTGGGA CGACATATCG ATTACCGACG TCGCCAGCAT CAAGCCCGAG 2280AACCTGACCG ACAGCGAGAT CAAGCAGATA TACAGTCGCT ACGGCATCAA GCTGGAGGAC 2340GGCATCCTGA TCGACAAGAA GGGCGGCATC CACTACGGCG AGTTCATCAA CGAGGCCAGC 2400TTCAACATCG AGCCCCTGCA GAACTACGTG ACCAAGTACG AGGTGACCTA CAGCAGCGAG 2460CTGGGCCCCA ACGTGAGCGA CACCCTGGAG AGCGACAAGA TTTACAAGGA CGGCACCATC 2520AAGTTCGACT TCACCAAGTA CAGCAAGAAC GAGCAGGGCC TGTTCTACGA CAGCGGCCTG 2580AACTGGGACT TCAAGATCAA CGCCATCACC TACGACGGCA AGGAGATGAA CGTGTTCCAC 2640CGCTACAACA AGTAG 2655

(2) about the information of SEQ ID NO:18

(ⅰ) sequence signature

(A) length: 2004 nucleotide.

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: DNA (genome)

(ⅲ) the false plan: not

(ⅳ) antisense: not

(ⅸ) feature:

(A) title/key: misc_feathure

(B) position: 1.2004

(D) out of Memory :/remarks=" from the corn optimization dna sequence dna of coding 80kd VIP1A (a) albumen of AB78.”

( ⅹⅰ ) ：SEQ ID NO:18:ATGAAGCGCG AGATCGACCA GGACACCCAC ACCGACGGCG ACAGCATCCC CGACCTGTGG 60GAGGAGAACG GCTACACCAT CCAGAACCCC ATCGCCGTGA AGTGGGACGA CAGCCTGGCT 120AGCAAGGGCT ACACCAAGTT CGTGAGCAAC CCCCTGGAGA CCCACACCGT GGGCGACCCC 180TACACCGACT ACGAGAAGCC CGCCCGCGAC CTGGACCTGA GCAACGCCAA GGAGACCTTC 240AACCCCCTGG TGGCCGCCTT CCCCAGCGTG AACGTGAGCA TGGAGAAGGT GATCCTGAGC 300CCCAACGAGA ACCTGAGCAA CAGCGTGGAG AGCCACTCGA GCACCAACTG GAGCTACACC 360AACACCGACG CCCCCAGCGT GGAGGCCGGC ATCGGTCCCA AGGGCATCAG CTTCGGCGTG 420AGCGTGAACT ACCAGCACAG CGAGACCGTG GCCCAGGAGT GGGGCACCAG CACCGGCAAC 480ACCAGCCAGT TCAACACCGC CAGCGCCGGC TACCTGAACG CCAACGTGCG CTACAACAAC 540GTGGGCACCG GCGCCATCTA CGACGTGAAG CCCACCACCA GCTTCGTGCT GAACAACGAC 600ACCATCCCCA CCATCACCGC CAAGTCGAAT TCCACCGCCC TGAACATCAG CCCCGGCGAG 660AGCTACCCCA AGAAGGGCCA GAACGGCATC GCCATCACCA GCATGGACGA CTTCAACACC 720CACCCCATCA CCCTGAACAA GAAGCAGGTG GACAACCTGC TGAACAACAA CCCCATGATG 780CTGCAGACCA ACCAGACCGA CGGCGTCTAC AAGATCAAGG ACACCCACGG CAACATCGTG 840ACCGGCGGCG AGTGGAACGG CGTGATCCAG CAGATCAAGG CCAAGACCGC CACCATCATC 900GTCGACGACG CCGAGCGCGT GCCCGAGAAG CGCGTGGCCG CCAAGGACTA CGAGAACCCC 960GACGACAAGA CCCCCAGCCT GACCCTGAAG GACCCCCTGA AGCTGAGCTA CCCCGACGAG 1020ATCAAGGAGA TCGAGGGCCT CCTGTACTAC AAGAACAAGC CCATCTACGA GAGCAGCGTG 1080ATGACCTATC TAGACGAGAA CACCGCCAAG GAGGTGACCA AGCAGCTGAA CGACACCACC 1140GCCAAGTTCA AGGACGTGAG CCACCTGTAC GACGTGAAGC TGACCCCCAA GATGAACGTG 1200ACCATCAAGC TGAGCATCCT GTACGACAAC GCCGAGACCA ACGACAACAG CATCGGCAAG 1260TGGACCAACA CCAACATCGT GAGCGGCGGC AACAACGGCA AGAAGCAGTA CACCACCAAC 1320AACCCCGACG CCAACCTGAC CCTGAACACC GACGCCCAGG AGAAGCTGAA CAAGAACCGC 1380GACTACTACA TCAGCCTGTA CATGAAGAGC GAGAAGAACA CCCAGTGCGA GATCACCATC 1440GACGGCGAGA TATACCCCAT CACCACCAAG ACCGTGAACG TGAACAAGGA CAACTACAAG 1500CGCCTGGACA TCATCGCCCA CAACATCAAG AGCAACCCCA TCAGCACCCT GCACATCAAG 1560ACCAACGACG AGATCACCCT GTTCTGGGAC GACATATCGA TTACCGACGT CGCCAGCATC 1620AAGCCCGAGA ACCTGACCGA CAGCGAGATC AAGCAGATAT ACAGTCGCTA CGGCATCAAG 1680CTGGAGGACG GCATCCTGAT CGACAAGAAG GGCGGCATCC ACTACGGCGA GTTCATCAAC 1740GAGGCCAGCT TCAACATCGA GCCCCTGCAG AACTACGTGA CCAAGTACGA GGTGACCTAC 1800AGCAGCGAGC TGGGCCCCAA CGTGAGCGAC ACCCTGGAGA GCGACAAGAT TTACAAGGAC 1860GGCACCATCA AGTTCGACTT CACCAAGTAC AGCAAGAACG AGCAGGGCCT GTTCTACGAC 1920AGCGGCCTGA ACTGGGACTT CAAGATCAAC GCCATCACCT ACGACGGCAA GGAGATGAAC 1980GTGTTCCACC GCTACAACAA GTAG 2004

(2) about the information of SEQ ID NO:19

(ⅰ) sequence signature

(A) length: 4074 nucleotide

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: DNA (genome)

(ⅸ) feature:

(A) title/key: CDS

(B) position: 1..1386

(D) out of Memory :/product=" from the VIP2A (b) of Btt "

(ⅸ) feature:

(A) title/key: CDS

(B) position: 1394.3895

(D) out of Memory :/product=" from the VIP1A (b) of Btt "

(ⅸ) feature:

(A) title/key: misc_feature

(B) position: 1..4074

(D) out of Memory :/remarks=" from the cloned dna sequence of the gene that contains coding VIP1A (b) and VIP2A (b) of Btt.”

(ⅹ ⅰ) sequence description: SEQ ID NO:19:ATG CAA AGA ATG GAG GGA AAG TTG TTT GTG GTG TCA AAA ACA TTA CAA 48Met Gln Arg Met Glu Gly Lys Leu Phe Val Val Ser Lys Thr Leu Gln

670?????????????????675?????????????????680GTA?GTT?ACT?AGA?ACT?GTA?TTG?CTT?AGT?ACA?GTT?TAC?TCT?ATA?ACT?TTA?????????96Val?Val?Thr?Arg?Thr?Val?Leu?Leu?Ser?Thr?Val?Tyr?Ser?Ile?Thr?Leu

685?????????????????690?????????????????695TTA?AAT?AAT?GTA?GTG?ATA?AAA?GCT?GAC?CAA?TTA?AAT?ATA?AAT?TCT?CAA?????????144Leu?Asn?Asn?Val?Val?Ile?Lys?Ala?Asp?Gln?Leu?Asn?Ile?Asn?Ser?Gln700?????????????????705?????????????????710?????????????????715AGT?AAA?TAT?ACT?AAC?TTG?CAA?AAT?CTA?AAA?ATC?CCT?GAT?AAT?GCA?GAG?????????192Ser?Lys?Tyr?Thr?Asn?Leu?Gln?Asn?Leu?Lys?Ile?Pro?Asp?Asn?Ala?Glu

720?????????????????725?????????????????730GAT?TTT?AAA?GAA?GAT?AAG?GGG?AAA?GCG?AAA?GAA?TGG?GGG?AAA?GAG?AAA?????????240Asp?Phe?Lys?Glu?Asp?Lys?Gly?Lys?Ala?Lys?Glu?Trp?Gly?Lys?Glu?Lys

735?????????????????740?????????????????745GGG?GAA?GAG?TGG?AGG?CCT?CCT?GCT?ACT?GAG?AAA?GGA?GAA?ATG?AAT?AAT?????????288Gly?Glu?Glu?Trp?Arg?Pro?Pro?Ala?Thr?Glu?Lys?Gly?Glu?Met?Asn?Asn

750?????????????????755?????????????????760TTT?TTA?GAT?AAT?AAA?AAT?GAT?ATA?AAG?ACC?AAT?TAT?AAA?GAA?ATT?ACT?????????336Phe?Leu?Asp?Asn?Lys?Asn?Asp?Ile?Lys?Thr?Asn?Tyr?Lys?Glu?Ile?Thr

765?????????????????770?????????????????775TTT?TCT?ATG?GCA?GGT?TCA?TGT?GAA?GAT?GAA?ATA?AAA?GAT?TTA?GAA?GAA?????????384Phe?Ser?Met?Ala?Gly?Ser?Cys?Glu?Asp?Glu?Ile?Lys?Asp?Leu?Glu?Glu780?????????????????785?????????????????790?????????????????795ATT?GAT?AAG?ATC?TIT?GAT?AAA?GCC?AAT?CTC?TCG?AGT?TCT?ATT?ATC?ACC?????????432Ile?Asp?Lys?Ile?Phe?Asp?Lys?Ala?Asn?Leu?Ser?Ser?Ser?Ile?Ile?Thr

800?????????????????805?????????????????810TAT?AAA?AAT?GTG?GAA?CCA?GCA?ACA?ATT?GGA?TTT?AAT?AAA?TCT?TTA?ACA?????????480Tyr?Lys?Asn?Val?Glu?Pro?Ala?Thr?Ile?Gly?Phe?Asn?Lys?Ser?Leu?Thr

815?????????????????820?????????????????825GAA?GGT?AAT?ACG?ATT?AAT?TCT?GAT?GCA?ATG?GCA?CAG?TTT?AAA?GAA?CAA?????????528Glu?Gly?Asn?Thr?Ile?Asn?Ser?Asp?Ala?Met?Ala?Gln?Phe?Lys?Glu?Gln

830?????????????????835?????????????????840TTT?TTA?GGT?AAG?GAT?ATG?AAG?TTT?GAT?AGT?TAT?CTA?GAT?ACT?CAT?TTA?????????576Phe?Leu?Gly?Lys?Asp?Met?Lys?Phe?Asp?Ser?Tyr?Leu?Asp?Thr?His?Leu

845?????????????????850?????????????????855ACT?GCT?CAA?CAA?GTT?TCC?AGT?AAA?AAA?AGA?GTT?ATT?TTG?AAG?GTT?ACG?????????624Thr?Ala?Gln?Gln?Val?Ser?Ser?Lys?Lys?Arg?Val?Ile?Leu?Lys?Val?Thr860?????????????????865?????????????????870?????????????????875GTT?CCG?AGT?GGG?AAA?GGT?TCT?ACT?ACT?CCA?ACA?AAA?GCA?GGT?GTC?ATT?????????672Val?Pro?Ser?Gly?Lys?Gly?Ser?Thr?Thr?Pro?Thr?Lys?Ala?Gly?Val?Ile

880?????????????????885?????????????????890TTA?AAC?AAT?AAT?GAA?TAC?AAA?ATG?CTC?ATT?GAT?AAT?GGG?TAT?GTG?CTC?????????720Leu?Asn?Asn?Asn?Glu?Tyr?Lys?Met?Leu?Ile?Asp?Asn?Gly?Tyr?Val?Leu

895?????????????????900?????????????????905CAT?GTA?GAT?AAG?GTA?TCA?AAA?GTA?GTA?AAA?AAA?GGG?ATG?GAG?TGC?TTA????????768His?Val?Asp?Lys?Val?Ser?Lys?Val?Val?Lys?Lys?Gly?Met?Glu?Cys?Leu

910?????????????????915?????????????????920CAA?GTT?GAA?GGG?ACT?TTA?AAA?AAG?AGT?CTC?GAC?TTT?AAA?AAT?GAT?ATA????????816Gln?Val?Glu?Gly?Thr?Leu?Lys?Lys?Ser?Leu?Asp?Phe?Lys?Asn?Asp?Ile

925?????????????????930?????????????????935AAT?GCT?GAA?GCG?CAT?AGC?TGG?GGG?ATG?AAA?ATT?TAT?GAA?GAC?TGG?GCT????????864Asn?Ala?Glu?Ala?His?Ser?Trp?Gly?Met?Lys?Ile?Tyr?Glu?Asp?Trp?Ala940?????????????????945?????????????????950?????????????????955AAA?AAT?TTA?ACC?GCT?TCG?CAA?AGG?GAA?GCT?TTA?GAT?GGG?TAT?GCT?AGG????????912Lys?Asn?Leu?Thr?Ala?Ser?Gln?Arg?Glu?Ala?Leu?Asp?Gly?Tyr?Ala?Arg

960?????????????????965?????????????????970CAA?GAT?TAT?AAA?GAA?ATC?AAT?AAT?TAT?TIG?CGC?AAT?CAA?GGC?GGG?AGT????????960Gln?Asp?Tyr?Lys?Glu?Ile?Asn?Asn?Tyr?Leu?Arg?Asn?Gln?Gly?Gly?Ser

975?????????????????980?????????????????985GGA?AAT?GAA?AAG?CIG?GAT?GCC?CAA?TTA?AAA?AAT?ATT?TCT?GAT?GCT?TTA???????1008Gly?Asn?Glu?Lys?Leu?Asp?Ala?Gln?Leu?Lys?Asn?Ile?Ser?Asp?Ala?Leu

990?????????????????995?????????????????1000GGG?AAG?AAA?CCC?ATA?CCA?GAA?AAT?ATT?ACC?GTG?TAT?AGA?TGG?TGT?GGC???????1056Gly?Lys?Lys?Pro?Ile?Pro?Glu?Asn?Ile?Thr?Val?Tyr?Arg?Trp?Cys?Gly

1005????????????????1010????????????????1015ATG?CCG?GAA?TTT?GGT?TAT?CAA?ATT?AGT?GAT?CCG?TTA?CCT?TCT?TTA?AAA???????1104Met?Pro?Glu?Phe?Gly?Tyr?Gln?Ile?Ser?Asp?Pro?Leu?Pro?Ser?Leu?Lys1020????????????????1025????????????????1030????????????????1035GAT?TTT?GAA?GAA?CAA?TTT?TTA?AAT?ACA?ATT?AAA?GAA?GAC?AAA?GGG?TAT???????1152Asp?Phe?Glu?Glu?Gln?Phe?Leu?Asn?Thr?Ile?Lys?Glu?Asp?Lys?Gly?Tyr

1040????????????????1045????????????????1050ATG?AGT?ACA?AGC?TTA?TCG?AGT?GAA?CGT?CTT?GCA?GCT?TTT?GGA?TCT?AGA???????1200Met?Ser?Thr?Ser?Leu?Ser?Ser?Glu?Arg?Leu?Ala?Ala?Phe?Gly?Ser?Arg

1055?????????????????1060????????????????1065AAA?ATT?ATA?TTA?CGC?TTA?CAA?GTT?CCG?AAA?GGA?AGT?ACG?GGG?GCG?TAT???????1248Lys?Ile?Ile?Leu?Arg?Leu?Gln?Val?Pro?Lys?Gly?Ser?Thr?Gly?Ala?Tyr

1070????????????????1075????????????????1080TTA?AGT?GCC?ATT?GGT?GGA?TTT?GCA?AGT?GAA?AAA?GAG?ATC?CTA?CTT?GAT???????1296Leu?Ser?Ala?Ile?Gly?Gly?Phe?Ala?Ser?Glu?Lys?Glu?Ile?Leu?Leu?Asp

1085????????????????1090????????????????1095AAA?GAT?AGT?AAA?TAT?CAT?ATT?GAT?AAA?GCA?ACA?GAG?GTA?ATC?ATT?AAA???????1344Lys?Asp?Ser?Lys?Tyr?His?Ile?Asp?Lys?Ala?Thr?Glu?Val?Ile?Ile?Lys1100????????????????1105????????????????1110????????????????1115GGT?GTT?AAG?CGA?TAT?GTA?GTG?GAT?GCA?ACA?TTA?TTA?ACA?AAT???????????????1386Gly?Val?Lys?Arg?Tyr?Val?Val?Asp?Ala?Thr?Leu?Leu?Thr?Asn

1120????????????????1125TAAGGAG?ATG?AAA?AAT?ATG?AAG?AAA?AAG?TTA?GCA?AGT?GTT?GTA?ACC?TGT???????1435

Met?Lys?Asn?Met?Lys?Lys?Lys?Leu?Ala?Ser?Val?Val?Thr?Cys

1????????????????5??????????????????10ATG?TTA?TTA?GCT?CCT?ATG?TTT?TTG?AAT?GGA?AAT?GTG?AAT?GCT?GTT?AAC??????1483Met?Leu?Leu?Ala?Pro?Met?Phe?Leu?Asn?Gly?Asn?Val?Asn?Ala?Val?Asn?15??????????????????20??????????????????25??????????????????30GCG?GAT?AGT?AAA?ATA?AAT?CAG?ATT?TCT?ACA?ACG?CAG?GAA?AAC?CAA?CAG??????1531Ala?Asp?Ser?Lys?Ile?Asn?Gln?Ile?Ser?Thr?Thr?Gln?Glu?Asn?Gln?Gln

35??????????????????40??????????????????45AAA?GAG?ATG?GAC?CGA?AAG?GGA?TTA?TTG?GGA?TAT?TAT?TTC?AAA?GGA?AAA??????1579Lys?Glu?Met?Asp?Arg?Lys?Gly?Leu?Leu?Gly?Tyr?Tyr?Phe?Lys?Gly?Lys

50??????????????????55??????????????????60GAT?TTT?AAT?AAT?CTT?ACT?ATG?TTT?GCA?CCG?ACA?CGT?GAT?AAT?ACC?CTT??????1627Asp?Phe?Asn?Asn?Leu?Thr?Met?Phe?Ala?Pro?Thr?Arg?Asp?Asn?Thr?Leu

65??????????????????70??????????????????75ATG?TAT?GAC?CAA?CAA?ACA?GCG?AAT?GCA?TTA?TTA?GAT?AAA?AAA?CAA?CAA??????1675Met?Tyr?Asp?Gln?Gln?Thr?Ala?Asn?Ala?Leu?Leu?Asp?Lys?Lys?Gln?Gln

80??????????????????85??????????????????90GAA?TAT?CAG?TCC?ATT?CGT?TGG?ATT?GGT?TTG?ATT?CAG?CGT?AAA?GAA?ACG??????1723Glu?Tyr?Gln?Ser?Ile?Arg?Trp?Ile?Gly?Leu?Ile?Gln?Arg?Lys?Glu?Thr?95?????????????????100?????????????????105?????????????????110GGC?GAT?TTC?ACA?TTT?AAC?TTA?TCA?AAG?GAT?GAA?CAG?GCA?ATT?ATA?GAA??????1771Gly?Asp?Phe?Thr?Phe?Asn?Leu?Ser?Lys?Asn?Glu?Gln?Ala?Ile?Ile?Glu

115?????????????????120?????????????????125ATC?GAT?GGG?AAA?ATC?ATT?TCT?AAT?AAA?GGG?AAA?GAA?AAG?CAA?GTT?GTC??????1819Ile?AsP?Gly?Lys?Ile?Ile?Ser?Asn?Lys?Gly?Lys?Glu?Lys?Gln?Val?Val

130?????????????????135?????????????????140CAT?TTA?GAA?AAA?GAA?AAA?TTA?GTT?CCA?ATC?AAA?ATA?GAG?TAT?CAA?TCA??????1867His?Leu?Glu?Lys?Glu?Lys?Leu?Val?Pro?Ile?Lys?Ile?Glu?Tyr?Gln?Ser

145?????????????????150?????????????????155

GAT?ACG?AAA?TTT?AAT?ATT?GAT?AGT?AAA?ACA?TTT?AAA?GAA?CTT?AAA?TTA??????1915Asp?Thr?Lys?Phe?Asn?Ile?Asp?Ser?Lys?Thr?Phe?Lys?Glu?Leu?Lys?Leu

160?????????????????165?????????????????170TTT?AAA?ATA?GAT?AGT?CAA?AAC?CAA?TCT?CAA?CAA?GTT?CAA?CTG?AGA?AAC??????1963Phe?Lys?Ile?Asp?Ser?Gln?Asn?Gln?Ser?Gln?Gln?Val?Gln?Leu?Arg?Asn175?????????????????180?????????????????185?????????????????190CCT?GAA?TTT?AAC?AAA?AAA?GAA?TCA?CAG?GAA?TTT?TTA?GCA?AAA?GCA?TCA??????2011Pro?Glu?Phe?Asn?Lys?Lys?Glu?Ser?Gln?Glu?Phe?Leu?Ala?Lys?Ala?Ser

195?????????????????200?????????????????205AAA?ACA?AAC?CTT?TTT?AAG?CAA?AAA?ATG?AAA?AGA?GAT?ATT?GAT?GAA?GAT??????2059Lys?Thr?Asn?Leu?Phe?Lys?Gln?Lys?Met?Lys?Arg?Asp?Ile?Asp?Glu?Asp

210?????????????????215?????????????????220ACG?GAT?ACA?GAT?GGA?GAC?TCC?ATT?CCT?GAT?CTT?TGG?GAA?GAA?AAT?GGG??????2107Thr?Asp?Thr?Asp?Gly?Aso?Ser?Ile?Pro?Asp?Leu?Trp?Glu?Glu?Asn?Gly

225?????????????????230?????????????????235TAC?ACG?ATT?CAA?AAT?AAA?GTT?GCT?GTC?AAA?TGG?GAT?GAT?TCG?CTA?GCA??????2155Tyr?Thr?Ile?Gln?Asn?Lys?Val?Ala?Val?Lys?Trp?Asp?Asp?Ser?Leu?Ala

240?????????????????245?????????????????250AGT?AAG?GGA?TAT?ACA?AAA?TTT?GTT?TCG?AAT?CCA?TTA?GAC?AGC?CAC?ACA??????2203Ser?Lys?Gly?Tyr?Thr?Lys?Phe?Val?Ser?Asn?Pro?Leu?Asp?Ser?His?Thr255?????????????????260?????????????????265?????????????????270GTT?GGC?GAT?CCC?TAT?ACT?GAT?TAT?GAA?AAG?GCC?GCA?AGG?GAT?TTA?GAT??????2251Val?Gly?Asp?Pro?Tyr?Thr?Asp?Tyr?Glu?Lys?Ala?Ala?Arg?Asp?Leu?Asp

275?????????????????280?????????????????285TTA?TCA?AAT?GCA?AAG?GAA?ACG?TTC?AAC?CCA?TTG?GTA?GCT?GCT?TTT?CCA??????2299Leu?Ser?Asn?Ala?Lys?Glu?Thr?Phe?Asn?Pro?Leu?Val?Ala?Ala?Phe?Pro

290?????????????????295?????????????????300AGT?GTG?AAT?GTT?AGT?ATG?GAA?AAG?GTG?ATA?TTA?TCA?CCA?AAT?GAA?AAT??????2347Ser?Val?Asn?Val?Ser?Met?Glu?Lys?Val?Ile?Leu?Ser?Pro?Asn?Glu?Asn

305?????????????????310?????????????????315TTA?TCC?AAT?AGT?GTA?GAG?TCT?CAT?TCA?TCC?ACG?AAT?TGG?TCT?TAT?ACG??????2395Leu?Ser?Asn?Ser?Val?Glu?Ser?His?Ser?Ser?Thr?Asn?Trp?Ser?Tyr?Thr

320?????????????????325?????????????????330AAT?ACA?GAA?GGA?GCT?TCC?ATT?GAA?GCT?GGT?GGC?GGT?CCA?TTA?GGC?CTT??????2443Asn?Thr?Glu?Gly?Ala?Ser?Ile?Glu?Ala?Gly?Gly?Gly?Pro?Leu?Gly?Leu335?????????????????340?????????????????345?????????????????350TCT?TTT?GGC?GTG?AGT?GTT?ACT?TAT?CAA?CAC?TCT?GAA?ACA?GTT?GCA?CAA??????2491Ser?Phe?Gly?Val?Ser?Val?Thr?Tyr?Gln?His?Ser?Glu?Thr?Val?Ala?Gln

355?????????????????360?????????????????365GAA?TGG?GGA?ACA?TCT?ACA?GGA?AAT?ACT?TCA?CAA?TTC?AAT?ACG?GCT?TCA??????2539Glu?Trp?Gly?Thr?Ser?Thr?Gly?Asn?Thr?Ser?Gln?Phe?Asn?Thr?Ala?Ser

370?????????????????375?????????????????380GCG?GGA?TAT?TTA?AAT?GCA?AAT?GTT?CGG?TAT?AAC?AAT?GTA?GGG?ACT?GGT??????2587Ala?Gly?Tyr?Leu?Asn?Ala?Asn?Val?Arg?Tyr?Asn?Asn?Val?Gly?Thr?Gly

385?????????????????390?????????????????395GGC?ATC?TAT?GAT?GTA?AAA?CCT?ACA?ACA?AGT?TTT?GTA?TTA?AAT?AAC?AAT??????2635Ala?Ile?Tyr?Asp?Val?Lys?Pro?Thr?Thr?Ser?Phe?Val?Leu?Asn?Asn?Asn

400?????????????????405?????????????????410ACC?ATC?GCA?ACG?ATT?ACA?GCA?AAA?TCA?AAT?TCA?ACA?GCT?TTA?CGT?ATA??????2683Thr?Ile?Ala?Thr?Ile?Thr?Ala?Lys?Ser?Asn?Ser?Thr?Ala?Leu?Arg?Ile415?????????????????420?????????????????425?????????????????430TCT?CCG?GGG?GAT?AGT?TAT?CCA?GAA?ATA?GGA?GAA?AAC?GCT?ATT?GCG?ATT??????2731Ser?Pro?Gly?Asp?Ser?Tyr?Pro?Glu?Ile?Gly?Glu?Asn?Ala?Ile?Ala?Ile

435?????????????????440?????????????????445ACA?TCT?ATG?GAT?GAT?TTT?AAT?TCT?CAT?CCA?ATT?ACA?TTA?AAT?AAA?CAA??????2779Thr?Ser?Met?Asp?Asp?Phe?Asn?Ser?His?Pro?Ile?Thr?Leu?Asn?Lys?Gln

450?????????????????455?????????????????460CAG?GTA?AAT?CAA?TTG?ATA?AAT?AAT?AAG?CCA?ATT?ATG?CPA?GAG?ACA?GAC???????2827Gln?Val?Asn?Gln?Leu?Ile?Asn?Asn?Lys?Pro?Ile?Met?Leu?Glu?Thr?Asp

465?????????????????470?????????????????475CAA?ACA?GAT?GGT?GTT?TAT?AAA?ATA?AGA?GAT?ACA?CAT?GGA?AAT?ATT?GTA???????2875Gln?Thr?Asp?Gly?Val?Tyr?Lys?Ile?Arg?Asp?Thr?His?Gly?Asn?Ile?Val

480?????????????????485?????????????????490ACT?GGT?GGA?GAA?TGG?AAT?GGT?GTA?ACA?CAA?CAA?ATT?AAA?GCA?AAA?ACA???????2923Thr?Gly?Gly?Glu?Trp?Asn?Gly?Val?Thr?Gln?Gln?Ile?Lys?Ala?Lys?Thr495?????????????????500?????????????????505?????????????????510GCG?TCT?ATT?ATT?GTG?GAT?GAC?GGG?AAA?CAG?GTA?GCA?GAA?AAA?CGT?GTG???????2971Ala?Ser?Ile?Ile?Val?Asp?Asp?Gly?Lys?Gln?Val?Ala?Glu?Lys?Arg?Val

515?????????????????520?????????????????525GCG?GCA?AAA?GAT?TAT?GGT?CAT?CCA?GAA?GAT?AAA?ACA?CCA?CCT?TTA?ACT???????3019Ala?Ala?Lys?Asp?Tyr?Gly?His?Pro?Glu?Asp?Lys?Thr?Pro?Pro?Leu?Thr

530?????????????????535?????????????????540TTA?AAA?GAT?ACC?CTG?AAG?CTT?TCA?TAC?CCA?GAT?GAA?ATA?AAA?GAA?ACT???????3067Leu?Lys?Asp?Thr?Leu?Lys?Leu?Ser?Tyr?Pro?Asp?Glu?Ile?Lys?Glu?Thr

545?????????????????550?????????????????555AAT?GGA?TTG?TTG?TAC?TAT?GAT?GAC?AAA?CCA?ATC?TAT?GAA?TCG?AGT?GTC???????3115Asn?Gly?Leu?Leu?Tyr?Tyr?Asp?Asp?Lys?Pro?Ile?Tyr?Glu?Ser?Ser?Val

560?????????????????565?????????????????570ATG?ACT?TAT?CTG?GAT?GAA?AAT?ACG?GCA?AAA?GAA?GTC?AAA?AAA?CAA?ATA???????3163Met?Thr?Tyr?Leu?Asp?Glu?Asn?Thr?Ala?Lys?Glu?Val?Lys?Lys?Gln?Ile575?????????????????580?????????????????585?????????????????590

AAT?GAT?ACA?ACC?GGA?AAA?TTT?AAG?GAT?GTA?AAT?CAC?TTA?TAT?GAT?GTA???????3211Asn?Asp?Thr?Thr?Gly?Lys?Phe?Lys?Asp?Val?Asn?His?Leu?Tyr?Asp?Val

595?????????????????600?????????????????605AAA?CTG?ACT?CCA?AAA?ATG?AAT?TTT?ACG?ATT?AAA?ATG?GCT?TCC?TTG?TAT???????3259Lys?Leu?Thr?Pro?Lys?Met?Asn?Phe?Thr?Ile?Lys?Met?Ala?Ser?Leu?Tyr

610?????????????????615?????????????????620GAT?GGG?GCT?GAA?AAT?AAT?CAT?AAC?TCT?TTA?GGA?ACC?TGG?TAT?TTA?ACA???????3307Asp?Gly?Ala?Glu?Asn?Asn?His?Asn?Ser?Leu?Gly?Thr?Trp?Tyr?Leu?Thr

625?????????????????630?????????????????635TAT?AAT?GTT?GCT?GGT?GGA?AAT?ACT?GGG?AAG?AGA?CAA?TAT?CGT?TCA?GCT???????3355Tyr?Asn?Val?Ala?Gly?Gly?Asn?Thr?Gly?Lys?Arg?Gln?Tyr?Arg?Ser?Ala

640?????????????????645?????????????????650CAT?TCT?TGT?GCA?CAT?GTA?GCT?CTA?TCT?TCA?GAA?GCG?AAA?AAG?AAA?CTA???????3403His?Ser?Cys?Ala?His?Val?Ala?Leu?Ser?Ser?Glu?Ala?Lys?Lys?Lys?Leu655?????????????????660?????????????????665?????????????????670AAT?CAA?AAT?GCG?AAT?TAC?TAT?CTT?AGC?ATG?TAT?ATG?AAG?GCT?GAT?TCT???????3451Asn?Gln?Asn?Ala?Asn?Tyr?Tyr?Leu?Ser?Met?Tyr?Met?Lys?Ala?Asp?Ser

675?????????????????680?????????????????685ACT?ACG?GAA?CCT?ACA?ATA?GAA?GTA?GCT?GGG?GAA?AAA?TCT?GCA?ATA?ACA??????3499Thr?Thr?Glu?Pro?Thr?Ile?Glu?Val?Ala?Gly?Glu?Lys?Ser?Ala?Ile?Thr

690?????????????????695?????????????????700AGT?AAA?AAA?GTA?AAA?TTA?AAT?AAT?CAA?AAT?TAT?CAA?AGA?GTT?GAT?ATT??????3547Ser?Lys?Lys?Val?Lys?Leu?Asn?Asn?Gln?Asn?Tyr?Gln?Arg?Val?Asp?Ile

705?????????????????710?????????????????715TTA?GTG?AAA?AAT?TCT?GAA?AGA?AAT?CCA?ATG?GAT?AAA?ATA?TAT?ATA?AGA??????3595Leu?Val?Lys?Asn?Ser?Glu?Arg?Asn?Pro?Met?Asp?Lys?Ile?Tyr?Ile?Arg

720?????????????????725?????????????????730GGA?AAT?GGC?ACG?ACA?AAT?GTT?TAT?GGG?GAT?GAT?GTT?ACT?ATC?CCA?GAG??????3643Gly?Asn?Gly?Thr?Thr?Asn?Val?Tyr?Gly?Asp?Asp?Val?Thr?Ile?Pro?Glu735?????????????????740?????????????????745?????????????????750GTA?TCA?GCT?ATA?AAT?CCG?GCT?AGT?CTA?TCA?GAT?GAA?GAA?ATT?CAA?GAA??????3691Val?Ser?Ala?Ile?Asn?Pro?Ala?Ser?Leu?Ser?Asp?Glu?Glu?Ile?Gln?Glu

755?????????????????760?????????????????765ATA?TTT?AAA?GAC?TCA?ACT?ATT?GAA?TAT?GGA?AAT?CCT?AGT?TTC?GTT?GCT??????3739Ile?Phe?Lys?Asp?Ser?Thr?Ile?Glu?Tyr?Gly?Asn?Pro?Ser?Phe?Val?Ala

770?????????????????775?????????????????780GAT?GCC?GTA?ACA?TIT?AAA?AAT?ATA?AAA?CCT?TTA?CAA?AAT?TAT?GTA?AAG??????3787Asp?Ala?Val?Thr?Phe?Lys?Asn?Ile?Lys?Pro?Leu?Gln?Asn?Tyr?Val?Lys

785?????????????????790?????????????????795GAA?TAT?GAA?ATA?TAT?CAT?AAA?TCT?CAT?CGA?TAT?GAA?AAG?AAA?ACG?GTC??????3835Glu?Tyr?Glu?Ile?Tyr?His?Lys?Ser?His?Arg?Tyr?Glu?Lys?Lys?Thr?Val

800?????????????????805?????????????????810TIT?GAT?ATC?ATG?GGT?GTT?CAT?TAT?GAG?TAT?AGT?ATA?GCT?AGG?GAA?CAA??????3883Phe?Asp?Ile?Met?Gly?Val?His?Tyr?Glu?Tyr?Ser?Ile?Ala?Arg?Glu?Gln815?????????????????820?????????????????825?????????????????830AAG?AAA?GCC?GCA?TAATTTTAAA?AATAAAACTC?GTTAGAGTTT?ATTTAGCATG??????????3935Lys?Lys?Ala?AlaGTATTTTTAA?GAATAATCAA?TATGTTGAAC?CGTTTGTAGC?TGTTTGGAA?GGGAATTTTCA????3995TTTTATTTGG?TCTCTTAAGT?TGATGGGCAT?GGGATATGTT?CAGCATCCAA?GCGTTTNGGG????4055GGTTANAAAA?TCCAATTTT?????????????????????????????????????????????????4074

(2) about the information of SEQ ID NO:20

(ⅰ) sequence signature

(A) length: 462 amino acid

(B) type: amino acid

(D) topological structure: line style

(ⅱ) molecule type: protein

(ⅹ ⅰ) sequence description: SEQ ID NO:20:Met Gln Arg Met Glu Gly Lys Leu Phe Val Val Ser Lys Thr Leu Gln 15 10 15Val Val Thr Arg Thr Val Leu Leu Ser Thr Val Tyr Ser Ile Thr Leu

20???????????????????25??????????????????30Leu?Asn?Asn?Val?Val?Ile?Lys?Ala?Asp?Gln?Leu?Asn?Ile?Asn?Ser?Gln

35??????????????????40??????????????????45Ser?Lys?Tyr?Thr?Asn?Leu?Gln?Asn?Leu?Lys?Ile?Pro?Asp?Asn?Ala?Glu

50??????????????????55??????????????????60Asp?Phe?Lys?Glu?Asp?Lys?Gly?Lys?Ala?Lys?Glu?Trp?Gly?Lys?Glu?Lys?65??????????????????70??????????????????75??????????????????80Gly?Glu?Glu?Trp?Arg?Pro?Pro?Ala?Thr?Glu?Lys?Gly?Glu?Met?Asn?Asn

85??????????????????90??????????????????95Phe?Leu?Asp?Asn?Lys?Asn?Asp?Ile?Lys?Thr?Asn?Tyr?Lys?Glu?Ile?Thr

100?????????????????105?????????????????110Phe?Ser?Met?Ala?Gly?Ser?Cys?Glu?Asp?Glu?Ile?Lys?Asp?Leu?Glu?Glu

115?????????????????120?????????????????125Ile?Asp?Lys?Ile?Phe?Asp?Lys?Ala?Asn?Leu?Ser?Ser?Ser?Ile?Ile?Thr

130?????????????????135?????????????????140Tyr?Lys?Asn?Val?Glu?Pro?Ala?Thr?Ile?Gly?Phe?Asn?Lys?Ser?Leu?Thr145?????????????????150?????????????????155?????????????????160Glu?Gly?Asn?Thr?Ile?Asn?Ser?Asp?Ala?Met?Ala?Gln?Phe?Lys?Glu?Gln

165?????????????????170?????????????????175Phe?Leu?Gly?Lys?Asp?Met?Lys?Phe?Asp?Ser?Tyr?Leu?Asp?Thr?His?Leu

180?????????????????185?????????????????190Thr?Ala?Gln?Gln?Val?Ser?Ser?Lys?Lys?Arg?Val?Ile?Leu?Lys?Val?Thr

195?????????????????200?????????????????205Val?Pro?Ser?Gly?Lys?Gly?Ser?Thr?Thr?Pro?Thr?Lys?Ala?Gly?Val?Ile

210?????????????????215?????????????????220Leu?Asn?Asn?Asn?Glu?Tyr?Lys?Met?Leu?Ile?Asp?Asn?Gly?Tyr?Val?Leu225?????????????????230?????????????????235?????????????????240His?Val?Asp?Lys?Val?Ser?Lys?Val?Val?Lys?Lys?Gly?Met?Glu?Cys?Leu

245?????????????????250?????????????????255?Gln?Val?Glu?Gly?Thr?Leu?Lys?Lys?Ser?Leu?Asp?Phe?Lys?Asn?Asp?Ile

260?????????????????265?????????????????270Asn?Ala?Glu?Ala?His?Ser?Trp?Gly?Met?Lys?Ile?Tyr?Glu?Asp?Trp?Ala

275?????????????????280?????????????????285Lys?Asn?Leu?Thr?Ala?Ser?Gln?Arg?Glu?Ala?Leu?Asp?Gly?Tyr?Ala?Arg

290?????????????????295?????????????????300Gln?Asp?Tyr?Lys?Glu?Ile?Asn?Asn?Tyr?Leu?Arg?Asn?Gln?Gly?Gly?Ser305?????????????????310?????????????????315?????????????????320?Gly?Asn?Glu?Lys?Leu?Asp?Ala?Gln?Leu?Lys?Asn?Ile?Ser?Asp?Ala?Leu

325?????????????????330?????????????????335Gly?Lys?Lys?Pro?Ile?Pro?Glu?Asn?Ile?Thr?Val?Tyr?Arg?Trp?Cys?Gly

340?????????????????345?????????????????350Met?Pro?Glu?Phe?Gly?Tyr?Gln?Ile?Ser?Asp?Pro?Leu?Pro?Ser?Leu?Lys

355?????????????????360?????????????????365Asp?Phe?Glu?Glu?Gln?Phe?Leu?Asn?Thr?Ile?Lys?Glu?Asp?Lys?Gly?Tyr

370?????????????????375?????????????????380Met?Ser?Thr?Ser?Leu?Ser?Ser?Glu?Arg?Leu?Ala?Ala?Phe?Gly?Ser?Arg385?????????????????390?????????????????395?????????????????400Lys?Ile?Ile?Leu?Arg?Leu?Gln?Val?Pro?Lys?Gly?Ser?Thr?Gly?Ala?Tyr

405?????????????????410?????????????????415Leu?Ser?Ala?Ile?Gly?Gly?Phe?Ala?Ser?Glu?Lys?Glu?Ile?Leu?Leu?Asp

420?????????????????425?????????????????430Lys?Asp?Ser?Lys?Tyr?His?Ile?Asp?Lys?Ala?Thr?Glu?Val?Ile?Ile?Lys

435?????????????????440?????????????????445Gly?Val?Lys?Arg?Tyr?Val?Val?Asp?Ala?Thr?Leu?Leu?Thr?Asn

450?????????????????455?????????????????460

(2) about the information of SEQ ID NO:21

(ⅰ) sequence signature

(A) length: 834 amino acid

(B) type: amino acid

(D) topological structure: line style

(ⅱ) molecule type: protein

(ⅹ ⅰ) sequence description: SEQ ID NO:21:Met Lys Asn Met Lys Lys Lys Leu Ala Ser Val Val Thr Cys Met Leu 15 10 15Leu Ala Pro Met Phe Leu Asn Gly Asn Val Asn Ala Val Asn Ala Asp

20??????????????????25??????????????????30Ser?Lys?Ile?Asn?Gln?Ile?Ser?Thr?Thr?Gln?Glu?Asn?Gln?Gln?Lys?Glu

35??????????????????40??????????????????45Met?Asp?Arg?Lys?Gly?Leu?Leu?Gly?Tyr?Tyr?Phe?Lys?Gly?Lys?Asp?Phe

50???????????????????55??????????????????60Asn?Asn?Leu?Thr?Met?Phe?Ala?Pro?Thr?Arg?Asp?Asn?Thr?Leu?Met?Tyr?65??????????????????70??????????????????75??????????????????80Asp?Gln?Gln?Thr?Ala?Asn?Ala?Leu?Leu?Asp?Lys?Lys?Gln?Gln?Glu?Tyr

85??????????????????90??????????????????95Gln?Ser?Ile?Arg?Trp?Ile?Gly?Leu?Ile?Gln?Arg?Lys?Glu?Thr?Gly?Asp

100?????????????????105?????????????????110Phe?Thr?Phe?Asn?Leu?Ser?Lys?Asp?Glu?Gln?Ala?Ile?Ile?Glu?Ile?Asp

115?????????????????120?????????????????125

Gly?Lys?Ile?Ile?Ser?Asn?Lys?Gly?Lys?Glu?Lys?Gln?Val?Val?His?Leu

130?????????????????135?????????????????140Glu?Lys?Glu?Lys?Leu?Val?Pro?Ile?Lys?Ile?Glu?Tyr?Gln?Ser?Asp?Thr145?????????????????150?????????????????155?????????????????160Lys?Phe?Asn?Ile?Asp?Ser?Lys?Thr?Phe?Lys?Glu?Leu?Lys?Leu?Phe?Lys

165?????????????????170?????????????????175Ile?Asp?Ser?Gln?Asn?Gln?Ser?Gln?Gln?Val?Gln?Leu?Arg?Asn?Pro?Glu

180?????????????????185?????????????????190Phe?Asn?Lys?Lys?Glu?Ser?Gln?Glu?Phe?Leu?Ala?Lys?Ala?Ser?Lys?Thr

195?????????????????200?????????????????205Asn?Leu?Phe?Lys?Gln?Lys?Met?Lys?Arg?Asp?Ile?Asp?Glu?Asp?Thr?Asp

210?????????????????215?????????????????220Thr?Asp?Gly?Asp?Ser?Ile?Pro?Asp?Leu?Trp?Glu?Glu?Asn?Gly?Tyr?Thr225?????????????????230?????????????????235?????????????????240Ile?Gln?Asn?Lys?Val?Ala?Val?Lys?Trp?Asp?Asp?Ser?Leu?Ala?Ser?Lys

245?????????????????250?????????????????255Gly?Tyr?Thr?Lys?Phe?Val?Ser?Asn?Pro?Leu?Asp?Ser?His?Thr?Val?Gly

260?????????????????265?????????????????270Asp?Pro?Tyr?Thr?Asp?Tyr?Glu?Lys?Ala?Ala?Arg?Asp?Leu?Asp?Leu?Ser

275?????????????????280?????????????????285Asn?Ala?Lys?Glu?Thr?Phe?Asn?Pro?Leu?Val?Ala?Ala?Phe?Pro?Ser?Val

290?????????????????295?????????????????300Asn?Val?Ser?Met?Glu?Lys?Val?Ile?Leu?Ser?Pro?Asn?Glu?Asn?Leu?Ser305?????????????????310?????????????????315?????????????????320Asn?Ser?Val?Glu?Ser?His?Ser?Ser?Thr?Asn?Trp?Ser?Tyr?Thr?Asn?Thr

325?????????????????330?????????????????335Glu?Gly?Ala?Ser?Ile?Glu?Ala?Gly?Gly?Gly?Pro?Leu?Gly?Leu?Ser?Phe

340?????????????????345?????????????????350Gly?Val?Ser?Val?Thr?Tyr?Gln?His?Ser?Glu?Thr?Val?Ala?Gln?Glu?Trp

355?????????????????360?????????????????365Gly?Thr?Ser?Thr?Gly?Asn?Thr?Ser?Gln?Phe?Asn?Thr?Ala?Ser?Ala?Gly

370?????????????????375?????????????????380Tyr?Leu?Asn?Ala?Asn?Val?Arg?Tyr?Asn?Asn?Val?Gly?Thr?Gly?Ala?Ile385?????????????????390?????????????????395?????????????????400Tyr?Asp?Val?Lys?Pro?Thr?Thr?Ser?Phe?Val?Leu?Asn?Asn?Asn?Thr?Ile

405?????????????????410?????????????????415Ala?Thr?Ile?Thr?Ala?Lys?Ser?Asn?Ser?Thr?Ala?Leu?Arg?Ile?Ser?Pro

420?????????????????425?????????????????430Gly?Asp?Ser?Tyr?Pro?Glu?Ile?Gly?Glu?Asn?Ala?Ile?Ala?Ile?Thr?Ser

435?????????????????440?????????????????445Met?Asp?Asp?Phe?Asn?Ser?His?Pro?Ile?Thr?Leu?Asn?Lys?Gln?Gln?Val

450?????????????????455?????????????????460Asn?Gln?Leu?Ile?Asn?Asn?Lys?Pro?Ile?Met?Leu?Glu?Thr?Asp?Gln?Thr465?????????????????470?????????????????475?????????????????480Asp?Gly?Val?Tyr?Lys?Ile?Arg?Asp?Thr?His?Gly?Asn?Ile?Val?Thr?Gly

485?????????????????490?????????????????495Gly?Glu?Trp?Asn?Gly?Val?Thr?Gln?Gln?Ile?Lys?Ala?Lys?Thr?Ala?Ser

500?????????????????505?????????????????510Ile?Ile?Val?Asp?Asp?Gly?Lys?Gln?Val?Ala?Glu?Lys?Arg?Val?Ala?Ala

515?????????????????520?????????????????525Lys?Asp?Tyr?Gly?His?Pro?Glu?Asp?Lys?Thr?Pro?Pro?Leu?Thr?Leu?Lys

530?????????????????535?????????????????540Asp?Thr?Leu?Lys?Leu?Ser?Tyr?Pro?Asp?Glu?Ile?Lys?Glu?Thr?Asn?Gly545?????????????????550?????????????????555?????????????????560Leu?Leu?Tyr?Tyr?Asp?Asp?Lys?Pro?Ile?Tyr?Glu?Ser?Ser?Val?Met?Thr

565?????????????????570?????????????????575Tyr?Leu?Asp?Glu?Asn?Thr?Ala?Lys?Glu?Val?Lys?Lys?Gln?Ile?Asn?Asp

580?????????????????585?????????????????590Thr?Thr?Gly?Lys?Phe?Lys?Asp?Val?Asn?His?Leu?Tyr?Asp?Val?Lys?Leu

595?????????????????600?????????????????605Thr?Pro?Lys?Met?Asn?Phe?Thr?Ile?Lys?Met?Ala?Ser?Leu?Tyr?Asp?Gly

610?????????????????615?????????????????620Ala?Glu?Asn?Asn?His?Asn?Ser?Leu?Gly?Thr?Trp?Tyr?Leu?Thr?Tyr?Asn625?????????????????630?????????????????635?????????????????640Val?Ala?Gly?Gly?Asn?Thr?Gly?Lys?Arg?Gln?Tyr?Arg?Ser?Ala?His?Ser

645?????????????????650?????????????????655Cys?Ala?His?Val?Ala?Leu?Ser?Ser?Glu?Ala?Lys?Lys?Lys?Leu?Asn?Gln

660?????????????????665?????????????????670Asn?Ala?Asn?Tyr?Tyr?Leu?Ser?Met?Tyr?Met?Lys?Ala?Asp?Ser?Thr?Thr

675?????????????????680?????????????????685Glu?Pro?Thr?Ile?Glu?Val?Ala?Gly?Glu?Lys?Ser?Ala?Ile?Thr?Ser?Lys

690?????????????????695?????????????????700Lys?Val?Lys?Leu?Asn?Asn?Gln?Asn?Tyr?Gln?Arg?Val?Asp?Ile?Leu?Val705?????????????????710?????????????????715?????????????????720Lys?Asn?Ser?Glu?Arg?Asn?Pro?Met?Asp?Lys?Ile?Tyr?Ile?Arg?Gly?Asn

725?????????????????730?????????????????735Gly?Thr?Thr?Asn?Val?Tyr?Gly?Asp?Asp?Val?Thr?Ile?Pro?Glu?Val?Ser

740?????????????????745?????????????????750Ala?Ile?Asn?Pro?Ala?Ser?Leu?Ser?Asp?Glu?Glu?Ile?Gln?Glu?Ile?Phe

755?????????????????760?????????????????765Lys?Asp?Ser?Thr?Ile?Glu?Tyr?Gly?Asn?Pro?Ser?Phe?Val?Ala?Asp?Ala???770??????????????????775?????????????????780Val?Thr?Phe?Lys?Asn?Ile?Lys?Pro?Leu?Gln?Asn?Tyr?Val?Lys?Glu?Tyr785?????????????????790?????????????????795?????????????????800Glu?Ile?Tyr?His?Lys?Ser?His?Arg?Tyr?Glu?Lys?Lys?Thr?Val?Phe?Asp

805?????????????????810?????????????????815Ile?Met?Gly?Val?His?Tyr?Glu?Tyr?Ser?Ile?Ala?Arg?Glu?Gln?Lys?Lys

820?????????????????825?????????????????830Ala?Ala

(2) about the information of SEQ ID NO:22

(ⅰ) sequence signature

(A) length: 4041 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: DNA (genome)

(ⅸ) feature:

(A) title/key: CDS

(B) position: 1.4038

(D) out of Memory :/product=" VIP1A (a) and VIP2A (a) fusion product "

(ⅹ ⅰ) sequence description: SEQ ID NO:22:ATG AAA AGA ATG GAG GGA AAG TTG TTT ATG GTG TCA AAA AAA TTA CAA 48Met Lys Arg Met Glu Gly Lys Leu Phe Met Val Ser Lys Lys Leu Gln835 840 845 850GTA GTT ACT AAA ACT GTA TTG CTT AGT ACA GTT TTC TCT ATA TCT TTA 96Val Val Thr Lys Thr Val Leu Leu Ser Thr Val Phe Ser Ile Ser Leu

855????????????????860??????????????????865TTA?AAT?AAT?GAA?GTG?ATA?AAA?GCT?GAA?CAA?TTA?AAT?ATA?AAT?TCT?CAA?????????144Leu?Asn?Asn?Glu?Val?Ile?Lys?Ala?Glu?Gln?Leu?Asn?Ile?Asn?Ser?Gln

870?????????????????875?????????????????880AGT?AAA?TAT?ACT?AAC?TTG?CAA?AAT?CTA?AAA?ATC?ACT?GAC?AAG?GTA?GAG?????????192Ser?Lys?Tyr?Thr?Asn?Leu?Gln?Asn?Leu?Lys?Ile?Thr?Asp?Lys?Val?Glu

885?????????????????890?????????????????895GAT?TTT?AAA?GAA?GAT?AAG?GAA?AAA?GCG?AAA?GAA?TGG?GGG?AAA?GAA?AAA?????????240Asp?Phe?Lys?Glu?Asp?Lys?Glu?Lys?Ala?Lys?Glu?Trp?Gly?Lys?Glu?Lys???900??????????????????905?????????????????910GAA?AAA?GAG?TGG?AAA?CTA?ACT?GCT?ACT?GAA?AAA?GGA?AAA?ATG?AAT?AAT?????????288Glu?Lys?Glu?Trp?Lys?Leu?Thr?Ala?Thr?Glu?Lys?Gly?Lys?Met?Asn?Asn915?????????????????920?????????????????925?????????????????930TTT?TTA?GAT?AAT?AAA?AAT?GAT?ATA?AAG?ACA?AAT?TAT?AAA?GAA?ATT?ACT?????????336Phe?Leu?Asp?Asn?Lys?Asn?Asp?Ile?Lys?Thr?Asn?Tyr?Lys?Glu?Ile?Thr

935?????????????????940?????????????????945TTT?TCT?ATG?GCA?GGC?TCA?TTT?GAA?GAT?GAA?ATA?AAA?GAT?TTA?AAA?GAA?????????384Phe?Ser?Met?Ala?Gly?Ser?Phe?Glu?Asp?Glu?Ile?Lys?Asp?Leu?Lys?Glu

950?????????????????955?????????????????960ATT?GAT?AAG?ATG?TTT?GAT?AAA?ACC?AAT?CTA?TCA?AAT?TCT?ATT?ATC?ACC?????????432Ile?Asp?Lys?Met?Phe?Asp?Lys?Thr?Asn?Leu?Ser?Asn?Ser?Ile?Ile?Thr

965?????????????????970?????????????????975TAT?AAA?AAT?GTG?GAA?CCG?ACA?ACA?ATT?GGA?TTT?AAT?AAA?TCT?TTA?ACA?????????480Tyr?Lys?Asn?Val?Glu?Pro?Thr?Thr?Ile?Gly?Phe?Asn?Lys?Set?Leu?Thr

980?????????????????985??????????????????990GAA?GGT?AAT?ACG?ATT?AAT?TCT?GAT?GCA?ATG?GCA?CAG?TTT?AAA?GAA?CAA?????????528Glu?Gly?Asn?Thr?Ile?Asn?Ser?Asp?Ala?Met?Ala?Gln?Phe?Lys?Glu?Gln995????????????????1000?????????????????1005????????????????1010TIT?TTA?GAT?AGG?GAT?ATT?AAG?TTT?GAT?AGT?TAT?CTA?GAT?ACG?CAT?TTA?????????576Phe?Leu?Asp?Arg?Asp?Ile?Lys?Phe?Asp?Ser?Tyr?Leu?Asp?Thr?His?Leu

1015????????????????1020????????????????1025ACT?GCT?CAA?CAA?GTT?TCC?AGT?AAA?GAA?AGA?GTT?ATT?TTG?AAG?GTT?ACG???????624Thr?Ala?Gln?Gln?Val?Ser?Ser?Lys?Glu?Arg?Val?Ile?Leu?Lys?Val?Thr

1030????????????????1035????????????????1040GTT?CCG?AGT?GGG?AAA?GGT?TCT?ACT?ACT?CCA?ACA?AAA?GCA?GGT?GTC?ATT???????672Val?Pro?Ser?Gly?Lys?Gly?Ser?Thr?Thr?Pro?Thr?Lys?Ala?Gly?Val?Ile

1045????????????????1050????????????????1055TTA?AAT?AAT?AGT?GAA?TAC?AAA?ATG?CTC?ATT?GAT?AAT?GGG?TAT?ATG?GTC???????720Leu?Asn?Asn?Ser?Glu?Tyr?Lys?Met?Leu?Ile?Asp?Asn?Gly?Tyr?Met?Val

1060????????????????1065????????????????1070CAT?GTA?GAT?AAG?GTA?TCA?AAA?GTG?GTG?AAA?AAA?GGG?GTG?GAG?TGC?TTA???????768His?Val?Asp?Lys?Val?Ser?Lys?Val?Val?Lys?Lys?Gly?Val?Glu?Cys?Leu1075????????????????1080????????????????1085????????????????1090?CAA?ATT?GAA?GGG?ACT?TTA?AAA?AAG?AGT?CTT?GAC?TTT?AAA?AAT?GAT?ATA???????816Gln?Ile?Glu?Gly?Thr?Leu?Lys?Lys?Ser?Leu?Asp?Phe?Lys?Asn?Asp?Ile

1095????????????????1100????????????????1105AAT?GCT?GAA?GCG?CAT?AGC?TGG?GGT?ATG?AAG?AAT?TAT?GAA?GAG?TGG?GCT???????864Asn?Ala?Glu?Ala?His?Ser?Trp?Gly?Met?Lys?Asn?Tyr?Glu?Glu?Trp?Ala

1110????????????????1115?????????????????1120AAA?GAT?TTA?ACC?GAT?TCG?CAA?AGG?GAA?GCT?TTA?GAT?GGG?TAT?GCT?AGG???????912Lys?Asp?Leu?Thr?Asp?Ser?Gln?Arg?Glu?Ala?Leu?Asp?Gly?Tyr?Ala?Arg

1125????????????????1130????????????????1135CAA?GAT?TAT?AAA?GAA?ATC?AAT?AAT?TAT?TTA?AGA?AAT?CAA?GGC?GGA?AGT???????960Gln?Asp?Tyr?Lys?Glu?Ile?Asn?Asn?Tyr?Leu?Arg?Asn?Gln?Gly?Gly?Ser

1140????????????????1145????????????????1150GGA?AAT?GAA?AAA?CTA?GAT?GCT?CAA?ATA?AAA?AAT?ATT?TCT?GAT?GCT?TTA??????1008Gly?Asn?Glu?Lys?Leu?Asp?Ala?Gln?Ile?Lys?Asn?Ile?Ser?Asp?Ala?Leu1155????????????????1160????????????????1165????????????????1170GGG?AAG?AAA?CCA?ATA?CCG?GAA?AAT?ATT?ACT?GTG?TAT?AGA?TGG?TGT?GGC??????1056Gly?Lys?Lys?Pro?Ile?Pro?Glu?Asn?Ile?Thr?Val?Tyr?Arg?Trp?Cys?Gly

1175????????????????1180????????????????1185ATG?CCG?GAA?TTT?GGT?TAT?CAA?ATT?AGT?GAT?CCG?TTA?CCT?TCT?TTA?AAA??????1104Met?Pro?Glu?Phe?Gly?Tyr?Gln?Ile?Ser?Asp?Pro?Leu?Pro?Ser?Leu?Lys

1190????????????????1195????????????????1200GAT?TTT?GAA?GAA?CAA?TTT?TTA?AAT?ACA?ATC?AAA?GAA?GAC?AAA?GGA?TAT??????1152Asp?Phe?Glu?Glu?Gln?Phe?Leu?Asn?Thr?Ile?Lys?Glu?Asp?Lys?Gly?Tyr

1205????????????????1210????????????????1215ATG?AGT?ACA?AGC?TTA?TCG?AGT?GAA?CGT?CTT?GCA?GCT?TTT?GGA?TCT?AGA??????1200Met?Ser?Thr?Ser?Leu?Ser?Ser?Glu?Arg?Leu?Ala?Ala?Phe?Gly?Ser?Arg

1220????????????????1225????????????????1230AAA?ATT?ATA?TTA?CGA?TTA?CAA?GTT?CCG?AAA?GGA?AGT?ACG?GGT?GCG?TAT??????1248Lys?Ile?Ile?Leu?Arg?Leu?Gln?Val?Pro?Lys?Gly?Ser?Thr?Gly?Ala?Tyr1235????????????????1240????????????????1245????????????????1250TTA?AGT?GCC?ATT?GGT?GGA?TIT?GCA?AGT?GAA?AAA?GAG?ATC?CTA?CTT?GAT???????1296Leu?Ser?Ala?Ile?Gly?Gly?Phe?Ala?Ser?Glu?Lys?Glu?Ile?Leu?Leu?Asp

1255????????????????1260????????????????????1265AAA?GAT?AGT?AAA?TAT?CAT?ATT?GAT?AAA?GTA?ACA?GAG?GTA?ATT?ATT?AAA???????1344Lys?Asp?Ser?Lys?Tyr?His?Ile?Asp?Lys?Val?Thr?Glu?Val?Ile?Ile?Lys

1270???????????????1275?????????????????????1280GGT?GTT?AAG?CGA?TAT?GTA?GTG?GAT?GCA?ACA?TTA?TTA?ACA?AAT?ATG?AAA???????1392Gly?Val?Lys?Arg?Tyr?Val?Val?Asp?Ala?Thr?Leu?Leu?Thr?Asn?Met?Lys

1285????????????????1290????????????????????1295AAT?ATG?AAG?AAA?AAG?TTA?GCA?AGT?GTT?GTA?ACG?TGT?ACG?TTA?TTA?GCT???????1440Asn?Met?Lys?Lys?Lys?Leu?Ala?Ser?Val?Val?Thr?Cys?Thr?Leu?Leu?Ala

1300????????????????1305????????????????????1310CCT?ATG?TTT?TTG?AAT?GGA?AAT?GTG?AAT?GCT?GTT?TAC?GCA?GAC?AGC?AAA???????1488Pro?Met?Phe?Leu?Asn?Gly?Asn?Val?Asn?Ala?Val?Tyr?Ala?Asp?Ser?Lys1315????????????????1320????????????????1325???????????????1330ACA?AAT?CAA?ATT?TCT?ACA?ACA?CAG?AAA?AAT?CAA?CAG?AAA?GAG?ATG?GAC???????1536Thr?Asn?Gln?Ile?Ser?Thr?Thr?Gln?Lys?Asn?Gln?Gln?Lys?Glu?Met?Asp

1335????????????????1340????????????????1345CGA?AAA?GGA?TTA?CTT?GGG?TAT?TAT?TTC?AAA?GGA?AAA?GAT?TTT?AGT?AAT???????1584Arg?Lys?Gly?Leu?Leu?Gly?Tyr?Tyr?Phe?Lys?Gly?Lys?Asp?Phe?Ser?Asn

1350????????????????1355????????????????1360CTT?ACT?ATG?TTT?GCA?CCG?ACA?CGT?GAT?AGT?ACT?CTT?ATT?TAT?GAT?CAA???????1632Leu?Thr?Met?Phe?Ala?Pro?Thr?Arg?Asp?Ser?Thr?Leu?Ile?Tyr?Asp?Gln

1365????????????????1370????????????????1375

CAA?ACA?GCA?AAT?AAA?CTA?TTA?GAT?AAA?AAA?CAA?CAA?GAA?TAT?CAG?TCT???????1680Gln?Thr?Ala?Asn?Lys?Leu?Leu?Asp?Lys?Lys?Gln?Gln?Glu?Tyr?Gln?Ser

1380????????????????1385????????????????1390ATT?CGT?TGG?ATT?GGT?TTG?ATT?CAG?AGT?AAA?GAA?ACG?GGA?GAT?TTC?ACA???????1728Ile?Arg?Trp?Ile?Gly?Leu?Ile?Gln?Ser?Lys?Glu?Thr?Gly?Asp?Phe?Thr1395????????????????1400????????????????1405????????????????1410TTT?AAC?TTA?TCT?GAG?GAT?GAA?CAG?GCA?ATT?ATA?GAA?ATC?AAT?GGG?AAA???????1776Phe?Asn?Leu?Ser?Glu?Asp?Glu?Gln?Ala?Ile?Ile?Glu?Ile?Asn?Gly?Lys

1415????????????????1420????????????????1425ATT?ATT?TCT?AAT?AAA?GGG?AAA?GAA?AAG?CAA?GTT?GTC?CAT?TTA?GAA?AAA???????1824Ile?Ile?Ser?Asn?Lys?Gly?Lys?Glu?Lys?Gln?Val?Val?His?Leu?Glu?Lys

1430????????????????1435????????????????1440GGA?AAA?TTA?GTT?CCA?ATC?AAA?ATA?GAG?TAT?CAA?TCA?GAT?ACA?AAA?TTT???????1872Gly?Lys?Leu?Val?Pro?Ile?Lys?Ile?Glu?Tyr?Gln?Ser?Asp?Thr?Lys?Phe

1445????????????????1450????????????????1455AAT?ATT?GAC?AGT?AAA?ACA?TTT?AAA?GAA?CTT?AAA?TTA?TTT?AAA?ATA?GAT???????1920Asn?Ile?Asp?Ser?Lys?Thr?Phe?Lys?Glu?Leu?Lys?Leu?Phe?Lys?Ile?Asp

1460????????????????1465????????????????1470AGT?CAA?AAC?CAA?CCC?CAG?CAA?GTC?CAG?CAA?GAT?GAA?CTG?AGA?AAT?CCT???????1968Ser?Gln?Asn?Gln?Pro?Gln?Gln?Val?Gln?Gln?Asp?Glu?Leu?Arg?Asn?Pro5???????????????????1480????????????????1485????????????????1490GAA?TTT?AAC?AAG?AAA?GAA?TCA?CAG?GAA?TTC?TTA?GCG?AAA?CCA?TCG?AAA???????2016Glu?Phe?Asn?Lys?Lys?Glu?Ser?Gln?Glu?Phe?Leu?Ala?Lys?Pro?Ser?Lys

1495????????????????1500????????????????1505ATA?AAT?CTT?TTC?ACT?CAA?AAA?ATG?AAA?AGG?GAA?ATT?GAT?GAA?GAC?ACG???????2064Ile?Asn?Leu?Phe?Thr?Gln?Lys?Met?Lys?Arg?Glu?Ile?Asp?Glu?Asp?Thr

1510????????????????1515????????????????1520GAT?ACG?GAT?GGG?GAC?TCT?ATT?CCT?GAC?CTT?TGG?GAA?GAA?AAT?GGG?TAT???????2112Asp?Thr?Asp?Gly?Asp?Ser?Ile?Pro?Asp?Leu?Trp?Glu?Glu?Asn?Gly?Tyr

1525????????????????1530????????????????1535ACG?ATT?CAA?AAT?AGA?ATC?GCT?GTA?AAG?TGG?GAC?GAT?TCT?CTA?GCA?AGT???????2160Thr?Ile?Gln?Asn?Arg?Ile?Ala?Val?Lys?Trp?Asp?Asp?Ser?Leu?Ala?Ser

1540????????????????1545????????????????1550AAA?GGG?TAT?ACG?AAA?TTT?GTT?TCA?AAT?CCA?CTA?GAA?AGT?CAC?ACA?GTT???????2208Lys?Gly?Tyr?Thr?Lys?Phe?Val?Ser?Asn?Pro?Leu?Glu?Ser?His?Thr?Val1555????????????????1560????????????????1565????????????????1570GGT?GAT?CCT?TAT?ACA?GAT?TAT?GAA?AAG?GCA?GCA?AGA?GAT?CTA?GAT?TTG???????2256Gly?Asp?Pro?Tyr?Thr?Asp?Tyr?Glu?Lys?Ala?Ala?Arg?Asp?Leu?Asp?Leu

1575????????????????1580????????????????1585TCA?AAT?GCA?AAG?GAA?ACG?TTT?AAC?CCA?TTG?GTA?GCT?GCT?TTP?CCA?AGT???????2304Ser?Asn?Ala?Lys?Glu?Thr?Phe?Asn?Pro?Leu?Val?Ala?Ala?Phe?Pro?Ser

1590????????????????1595????????????????1600GTG?AAT?GTT?AGT?ATG?GAA?AAG?GTG?ATA?TTA?TCA?CCA?AAT?GAA?AAT?TTA???????2352Val?Asn?Val?Ser?Met?Glu?Lys?Val?Ile?Leu?Ser?Pro?Asn?Glu?Asn?Leu

1605????????????????1610????????????????1615TCC?AAT?AGT?GTA?GAG?TCT?CAT?TCA?TCC?ACG?AAT?TGG?TCT?TAT?ACA?AAT???????2400Ser?Asn?Ser?Val?Glu?Ser?His?Ser?Ser?Thr?Asn?Trp?Ser?Tyr?Thr?Asn

1620????????????????1625????????????????1630ACA?GAA?GGT?GCT?TCT?GTT?GAA?GCG?GGG?ATT?GGA?CCA?AAA?GGT?ATT?TCG???????2448Thr?Glu?Gly?Ala?Ser?Val?Glu?Ala?Gly?Ile?Gly?Pro?Lys?Gly?Ile?Ser1635????????????????1640????????????????1645????????????????1650TTC?GGA?GTT?AGC?GTA?AAC?TAT?CAA?CAC?TCT?GAA?ACA?GTT?GCA?CAA?GAA???????2496Phe?Gly?Val?Ser?Val?Asn?Tyr?Gln?His?Ser?Glu?Thr?Val?Ala?Gln?Glu

1655????????????????1660????????????????1665TGG?GGA?ACA?TCT?ACA?GGA?AAT?ACT?TCG?CAA?TTC?AAT?ACG?GCT?TCA?GCG???????2544Trp?Gly?Thr?Ser?Thr?Gly?Asn?Thr?Ser?Gln?Phe?Asn?Thr?Ala?Ser?Ala

1670????????????????1675????????????????1680GGA?TAT?TTA?AAT?GCA?AAT?GTT?CGA?TAT?AAC?AAT?GTA?GGA?ACT?GGT?GCC???????2592Gly?Tyr?Leu?Asn?Ala?Asn?Val?Arg?Tyr?Asn?Asn?Val?Gly?Thr?Gly?Ala

1685????????????????1690????????????????1695ATC?TAC?GAT?GTA?AAA?CCT?ACA?ACA?AGT?TTT?GTA?TTA?AAT?AAC?GAT?ACT???????2640Ile?Tyr?Asp?Val?Lys?Pro?Thr?Thr?Ser?Phe?Val?Leu?Asn?Asn?Asp?Thr

1700????????????????1705????????????????1710ATC?GCA?ACT?ATT?ACG?GCG?AAA?TCT?AAT?TCT?ACA?GCC?TTA?AAT?ATA?TCT???????2688Ile?Ala?Thr?Ile?Thr?Ala?Lys?Ser?Asn?Ser?Thr?Ala?Leu?Asn?Ile?Ser1715????????????????1720????????????????1725????????????????1730CCT?GGA?GAA?AGT?TAC?CCG?AAA?AAA?GGA?CAA?AAT?GGA?ATC?GCA?ATA?ACA???????2736Pro?Gly?Glu?Ser?Tyr?Pro?Lys?Lys?Gly?Gln?Asn?Gly?Ile?Ala?Ile?Thr

1735????????????????1740????????????????1745TCA?ATG?GAT?GAT?TTT?AAT?TCC?CAT?CCG?ATT?ACA?TTA?AAT?AAA?AAA?CAA???????2784Ser?Met?Asp?Asp?Phe?Asn?Ser?His?Pro?Ile?Thr?Leu?Asn?Lys?Lys?Gln

1750????????????????1755????????????????1760GTA?GAT?AAT?CTG?CTA?AAT?AAT?AAA?CCT?ATG?ATG?TTG?GAA?ACA?AAC?CAA???????2832Val?Asp?Asn?Leu?Leu?Asn?Asn?Lys?Pro?Met?Met?Leu?Glu?Thr?Asn?Gln

1765????????????????1770????????????????1775ACA?GAT?GGT?GTT?TAT?AAG?ATA?AAA?GAT?ACA?CAT?GGA?AAT?ATA?GTA?ACT???????2880Thr?Asp?Gly?Val?Lys?Lys?Ile?Lys?Asp?Thr?His?Gly?Asn?Ile?Val?Thr

1780????????????????1785????????????????1790GGC?GGA?GAA?TGG?AAT?GGT?GTC?ATA?CAA?CAA?ATC?AAG?GCT?AAA?ACA?GCG???????2928Gly?Gly?Glu?Trp?Asn?Gly?Val?Ile?Gln?Gln?Ile?Lys?Ala?Lys?Thr?Ala1795????????????????1800????????????????1805????????????????1810TCT?ATT?ATT?GTG?GAT?GAT?GGG?GAA?CGT?GTA?GCA?GAA?AAA?CGT?GTA?GCG???????2976Ser?Ile?Ile?Val?Asp?Asp?Gly?Glu?Arg?Val?Ala?Glu?Lys?Arg?Val?Ala

1815????????????????1820????????????????1825GCA?AAA?GAT?TAT?GAA?AAT?CCA?GAA?GAT?AAA?ACA?CCG?TCT?TTA?ACT?TTA???????3024Ala?Lys?Asp?Tyr?Glu?Asn?Pro?Glu?Asp?Lys?Thr?Pro?Ser?Leu?Thr?Leu

1830????????????????1835????????????????1840AAA?GAT?GCC?CTG?AAG?CTT?TCA?TAT?CCA?GAT?GAA?ATA?AAA?GAA?ATA?GAG???????3072Lys?Asp?Ala?Leu?Lys?Leu?Ser?Tyr?Pro?Asp?Glu?Ile?Lys?Glu?Ile?Glu

1845????????????????1850????????????????1855GGA?TTA?TTA?TAT?TAT?AAA?AAC?AAA?CCG?ATA?TAC?GAA?TCG?AGC?GTT?ATG???????3120Gly?Leu?Leu?Tyr?Tyr?Lys?Asn?Lys?Pro?Ile?Tyr?Glu?Ser?Ser?Val?Met

1860????????????????1865????????????????1870ACT?TAC?TTA?GAT?GAA?AAT?ACA?GCA?AAA?GAA?GTG?ACC?AAA?CAA?TTA?AAT???????3168Thr?Tyr?Leu?Glu?Glu?Asn?Thr?Ala?Lys?Glu?Val?Thr?Lys?Gln?Leu?Asn1875????????????????1880????????????????1885????????????????1890GAT?ACC?ACT?GGG?AAA?TTT?AAA?GAT?GTA?AGT?CAT?TTA?TAT?GAT?GTA?AAA???????3216Asp?Thr?Thr?Gly?Lys?Phe?Lys?Asp?Val?Ser?His?Leu?Tyr?Asp?Val?Lys

1895????????????????1900????????????????1905CTG?ACT?CCA?AAA?ATG?AAT?GTT?ACA?ATC?AAA?TTG?TCT?ATA?CTT?TAT?GAT???????3264Leu?Thr?Pro?Lys?Met?Asn?Val?Thr?Ile?Lys?Leu?Ser?Ile?Leu?Tyr?Asp

1910????????????????1915????????????????1920AAT?GCT?GAG?TCT?AAT?GAT?AAC?TCA?ATT?GGT?AAA?TGG?ACA?AAC?ACA?AAT???????3312Asn?Ala?Glu?Ser?Asn?Asp?Asn?Ser?Ile?Gly?Lys?Trp?Thr?Asn?Thr?Asn

1925????????????????1930????????????????1935ATT?GTT?TCA?GGT?GGA?AAT?AAC?GGA?AAA?AAA?CAA?TAT?TCT?TCT?AAT?AAT???????3360Ile?Val?Ser?Gly?Gly?Asn?Asn?Gly?Lys?Lys?Gln?Tyr?Ser?Ser?Asn?Asn

1940????????????????1945???????????????1950CCG?GAT?GCT?AAT?TTG?ACA?TTA?AAT?ACA?GAT?GCT?CAA?GAA?AAA?TTA?AAT???????3408Pro?Asp?Ala?Asn?Leu?Thr?Leu?Asn?Thr?Asp?Ala?Gln?Glu?Lys?Leu?Asn1955????????????????1960????????????????1965????????????????1970AAA?AAT?CGT?GAC?TAT?TAT?ATA?AGT?TTA?TAT?ATG?AAG?TCA?GAA?AAA?AAC???????3456Lys?Asn?Arg?Asp?Tyr?Tyr?Ile?Ser?Leu?Tyr?Met?Lys?Ser?Glu?Lys?Asn

1975????????????????1980????????????????1985ACA?CAA?TGT?GAG?ATT?ACT?ATA?GAT?GGG?GAG?ATT?TAT?CCG?ATC?ACT?ACA???????3504Thr?Gln?Cys?Glu?Ile?Thr?Ile?Asp?Gly?Glu?Ile?Tyr?Pro?Ile?Thr?Thr

1990????????????????1995????????????????2000AAA?ACA?GTG?AAT?GTG?AAT?AAA?GAC?AAT?TAC?AAA?AGA?TTA?GAT?ATT?ATA???????3552Lys?Thr?Val?Asn?Val?Asn?Lys?Asp?Asn?Tyr?Lys?Arg?Leu?Asp?Ile?Ile

2005????????????????2010????????????????2015GCT?CAT?AAT?ATA?AAA?AGT?AAT?CCA?ATT?TCT?TCA?CTT?CAT?ATT?AAA?ACG???????3600Ala?His?Asn?Ile?Lys?Ser?Asn?Pro?Ile?Ser?Ser?Leu?His?Ile?Lys?Thr

2020????????????????2025????????????????2030AAT?GAT?GAA?ATA?ACT?TTA?TTT?TGG?GAT?GAT?ATT?TCT?ATA?ACA?GAT?GTA???????3648Asn?Asp?Glu?Ile?Thr?Leu?Phe?Trp?Asp?Asp?Ile?Ser?Ile?Thr?Asp?Val2035????????????????2040????????????????2045????????????????2050GCA?TCA?ATA?AAA?CCG?GAA?AAT?TTA?ACA?GAT?TCA?GAA?ATT?AAA?CAG?ATT???????3696Ala?Ser?Ile?Lys?Pro?Glu?Asn?Leu?Thr?Asp?Ser?Glu?Ile?Lys?Gln?Ile

2055????????????????2060????????????????2065TAT?AGT?AGG?TAT?GGT?ATT?AAG?TTA?GAA?GAT?GGA?ATC?CTT?ATT?GAT?AAA???????3744Tyr?Ser?Arg?Tyr?Gly?Ile?Lys?Leu?Glu?Asp?Gly?Ile?Leu?Ile?Asp?Lys

2070????????????????2075????????????????2080AAA?GGT?GGG?ATT?CAT?TAT?GGT?GAA?TTT?ATT?AAT?GAA?GCT?AGT?TIT?AAT???????3792Lys?Gly?Gly?Ile?His?Tyr?Gly?Glu?Phe?Ile?Asn?Glu?Ala?Ser?Phe?Asn

2085????????????????2090????????????????2095ATT?GAA?CCA?TTG?CAA?AAT?TAT?GTG?ACC?AAA?TAT?GAA?GTT?ACT?TAT?AGT???????3840Ile?Glu?Pro?Leu?Gln?Asn?Tyr?Val?Thr?Lys?Tyr?Glu?Val?Thr?Tyr?Ser

2100????????????????2105????????????????2110AGT?GAG?TTA?GGA?CCA?AAC?GTG?AGT?GAC?ACA?CTT?GAA?AGT?GAT?AAA?ATT???????3888Ser?Glu?Leu?Gly?Pro?Asn?Val?Ser?Asp?Thr?Leu?Glu?Ser?Asp?Lys?Ile2115????????????????2120????????????????2125????????????????2130TAC?AAG?GAT?GGG?ACA?ATT?AAA?TTT?GAT?TTT?ACC?AAA?TAT?AGT?AAA?AAT??????3936Tyr?Lys?Asp?Gly?Thr?Ile?Lys?Phe?Asp?Phe?Thr?Lys?Tyr?Ser?Lys?Asn

2135????????????????2140????????????????2145GAA?CAA?GGA?TTA?TTT?TAT?GAC?AGT?GGA?TTA?AAT?TGG?GAC?TTT?AAA?ATT??????3984Glu?Gln?Gly?Leu?Phe?Tyr?Asp?Ser?Gly?Leu?Asn?Trp?Asp?Phe?Lys?Ile

2150????????????????2155????????????????2160AAT?GCT?ATT?ACT?TAT?GAT?GGT?AAA?GAG?ATG?AAT?GTT?TTT?CAT?AGA?TAT??????4032Asn?Ala?Ile?Thr?Tyr?Asp?Gly?Lys?Glu?Met?Asn?Val?Phe?His?Arg?Tyr

2165????????????????2170????????????????2175AAT?AAA?TAG??????????????????????????????????????????????????????????4041Asn?Lys???2180

(2) about the information of SEQ ID NO:23

(ⅰ) sequence signature

(A) length: 1 346 amino acid

(B) type: amino acid

(D) topological structure: line style

(ⅱ) molecule type: protein

(ⅹ ⅰ) sequence description: SEQ ID NO:23:Met Lys Arg Met Glu Gly Lys Leu Phe Met Val Ser Lys Lys Leu Gln 15 10 15Val Val Thr Lys Thr Val Leu Leu Ser Thr Val Phe Ser Ile Ser Leu

20??????????????????25??????????????????30Leu?Asn?Asn?Glu?Val?Ile?Lys?Ala?Glu?Gln?Leu?Asn?Ile?Asn?Ser?Gln

35??????????????????40??????????????????45Ser?Lys?Tyr?Thr?Asn?Leu?Gln?Asn?Leu?Lys?Ile?Thr?Asp?Lys?Val?Glu

50??????????????????55??????????????????60Asp?Phe?Lys?Glu?Asp?Lys?Glu?Lys?Ala?Lys?Glu?Trp?Gly?Lys?Glu?Lys?65??????????????????70??????????????????75?????????????????80Glu?Lys?Glu?Trp?Lys?Leu?Thr?Ala?Thr?Glu?Lys?Gly?Lys?Met?Asn?Asn

85??????????????????90??????????????????95Phe?Leu?Asp?Asn?Lys?Asn?Asp?Ile?Lys?Thr?Asn?Tyr?Lys?Glu?Ile?Thr

100?????????????????105?????????????????110Phe?Ser?Met?Ala?Gly?Ser?Phe?Glu?Asp?Glu?Ile?Lys?Asp?Leu?Lys?Glu

115??????????????????120?????????????????125Ile?Asp?Lys?Met?Phe?Asp?Lys?Thr?Asn?Leu?Ser?Asn?Ser?Ile?Ile?Thr

130?????????????????135?????????????????140Tyr?Lys?Asn?Val?Glu?Pro?Thr?Thr?Ile?Gly?Phe?Asn?Lys?Ser?Leu?Thr145?????????????????150?????????????????155?????????????????160Glu?Gly?Asn?Thr?Ile?Asn?Ser?Asp?Ala?Met?Ala?Gln?Phe?Lys?Glu?Gln

165?????????????????170?????????????????175Phe?Leu?Asp?Arg?Asp?Ile?Lys?Phe?Asp?Ser?Tyr?Leu?Asp?Thr?His?Leu

18??????????????????185?????????????????190Thr?Ala?Gln?Gln?Val?Ser?Ser?Lys?Glu?Arg?Val?Ile?Leu?Lys?Val?Thr

195?????????????????200?????????????????205Val?Pro?Ser?Gly?Lys?Gly?Ser?Thr?Thr?Pro?Thr?Lys?Ala?Gly?Val?Ile

210?????????????????215?????????????????220Leu?Asn?Asn?Ser?Glu?Tyr?Lys?Met?Leu?Ile?Asp?Asn?Gly?Tyr?Met?Val225?????????????????230?????????????????235?????????????????240His?Val?Asp?Lys?Val?Ser?Lys?Val?Val?Lys?Lys?Gly?Val?Glu?Cys?Leu

245?????????????????250?????????????????255Gln?Ile?Glu?Gly?Thr?Leu?Lys?Lys?Ser?Leu?Asp?Phe?Lys?Asn?Asp?Ile

260?????????????????265?????????????????270Asn?Ala?Glu?Ala?His?Ser?Trp?Gly?Met?Lys?Asn?Tyr?Glu?Glu?Trp?Ala

275?????????????????280?????????????????285Lys?Asp?Leu?Thr?Asp?Ser?Gln?Arg?Glu?Ala?Leu?Asp?Gly?Tyr?Ala?Arg

290?????????????????295?????????????????300Gln?Asp?Tyr?Lys?Glu?Ile?Asn?Asn?Tyr?Leu?Arg?Asn?Gln?Gly?Gly?Ser305?????????????????310?????????????????315?????????????????320Gly?Asn?Glu?Lys?Leu?Asp?Ala?Gln?Ile?Lys?Asn?Ile?Ser?Asp?Ala?Leu

325?????????????????330?????????????????335Gly?Lys?Lys?Pro?Ile?Pro?Glu?Asn?Ile?Thr?Val?Tyr?Arg?Trp?Cys?Gly

340?????????????????345?????????????????350Met?Pro?Glu?Phe?Gly?Tyr?Gln?Ile?Ser?Asp?Pro?Leu?Pro?Ser?Leu?Lys

355?????????????????360?????????????????365Asp?Phe?Glu?Glu?Gln?Phe?Leu?Asn?Thr?Ile?Lys?Glu?Asp?Lys?Gly?Tyr

370?????????????????375?????????????????380Met?Ser?Thr?Ser?Leu?Ser?Ser?Glu?Arg?Leu?Ala?Ala?Phe?Gly?Ser?Arg385?????????????????390?????????????????395?????????????????400Lys?Ile?Ile?Leu?Arg?Leu?Gln?Val?Pro?Lys?GlM?Ser?Thr?Gly?Ala?Tyr

405?????????????????410?????????????????415Leu?Ser?Ala?Ile?Gly?Gly?Phe?Ala?Ser?Glu?Lys?Glu?Ile?Leu?Leu?Asp

420?????????????????425?????????????????430Lys?Asp?Ser?Lys?Tyr?His?Ile?Asp?Lys?Val?Thr?Glu?Val?Ile?Ile?Lys

435?????????????????440?????????????????445Gly?Val?Lys?Arg?Tyr?Val?Val?Asp?Ala?Thr?Leu?Leu?Thr?Asn?Met?Lys

450?????????????????455?????????????????460Asn?Met?Lys?Lys?Lys?Leu?Ala?Ser?Val?Val?Thr?Cys?Thr?Leu?Leu?Ala465?????????????????470?????????????????475?????????????????480Pro?Met?Phe?Leu?Asn?Gly?Asn?Val?Asn?Ala?Val?Tyr?Ala?Asp?Ser?Lys

485?????????????????490?????????????????495Thr?Asn?Gln?Ile?Ser?Thr?Thr?Gln?Lys?Asn?Gln?Gln?Lys?Glu?Met?Asp

500?????????????????505?????????????????510Arg?Lys?Gly?Leu?Leu?Gly?Tyr?Tyr?Phe?Lys?Gly?Lys?Asp?Phe?Ser?Asn

515?????????????????520?????????????????525Leu?Thr?Met?Phe?Ala?Pro?Thr?Arg?Asp?Ser?Thr?Leu?Ile?Tyr?Asp?Gln

530?????????????????535?????????????????540Gln?Thr?Ala?Asn?Lys?Leu?Leu?Asp?Lys?Lys?Gln?Gln?Glu?Tyr?Gln?Ser545????????????????550??????????????????555?????????????????560Ile?Arg?Trp?Ile?Gly?Leu?Ile?Gln?Ser?Lys?Glu?Thr?Gly?Asp?Phe?Thr

565?????????????????570?????????????????575Phe?Asn?Leu?Ser?Glu?Asp?Glu?Gln?Ala?Ile?Ile?Glu?Ile?Asn?Gly?Lys

580?????????????????585?????????????????590Ile?Ile?Ser?Asn?Lys?Gly?Lys?Glu?Lys?Gln?Val?Val?His?Leu?Glu?Lys

5???????????????????600?????????????????605Gly?Lys?Leu?Val?Pro?Ile?Lys?Ile?Glu?Tyr?Gln?Ser?Asp?Thr?Lys?Phe

610?????????????????615?????????????????620Asn?Ile?Asp?Ser?Lys?Thr?Phe?Lys?Glu?Leu?Lys?Leu?Phe?Lys?Ile?Asp625?????????????????630????????????????635??????????????????640Ser?Gln?Asn?Gln?Pro?Gln?Gln?Val?Gln?Gln?Asp?Glu?Leu?Arg?Asn?Pro

645?????????????????650?????????????????655Glu?Phe?Asn?Lys?Lys?Glu?Ser?Gln?Glu?Phe?Leu?Ala?Lys?Pro?Ser?Lys

660?????????????????665?????????????????670Ile?Asn?Leu?Phe?Thr?Gln?Lys?Met?Lys?Arg?Glu?Ile?Asp?Glu?Asp?Thr

675?????????????????680?????????????????685Asp?Thr?Asp?Gly?Asp?Ser?Ile?Pro?Asp?Leu?Trp?Glu?Glu?Asn?Gly?Tyr

690?????????????????695?????????????????700Thr?Ile?Gln?Asn?Arg?Ile?Ala?Val?Lys?Trp?Asp?Asp?Ser?Leu?Ala?Ser705?????????????????710?????????????????715?????????????????720Lys?Gly?Tyr?Thr?Lys?Phe?Val?Ser?Asn?Pro?Leu?Glu?Ser?His?Thr?Val

725?????????????????730?????????????????735Gly?Asp?Pro?Tyr?Thr?Asp?Tyr?Glu?Lys?Ala?Ala?Arg?Asp?Leu?Asp?Leu

740?????????????????745?????????????????750Ser?Asn?Ala?Lys?Glu?Thr?Phe?Asn?Pro?Leu?Val?Ala?Ala?Phe?Pro?Ser

755?????????????????760?????????????????765Val?Asn?Val?Ser?Met?Glu?Lys?Val?Ile?Leu?Ser?Pro?Asn?Glu?Asn?Leu

770?????????????????775?????????????????780Ser?Asn?Ser?Val?Glu?Ser?His?Ser?Ser?Thr?Asn?Trp?Ser?Tyr?Thr?Asn785?????????????????790?????????????????795?????????????????800Thr?Glu?Gly?Ala?Ser?Val?Glu?Ala?Gly?Ile?Gly?Pro?Lys?Gly?Ile?Ser

805??????????????????810????????????????815Phe?Gly?Val?Ser?Val?Asn?Tyr?Gln?His?Ser?Glu?Thr?Val?Ala?Gln?Glu

820?????????????????825?????????????????830Trp?Gly?Thr?Ser?Thr?Gly?Asn?Thr?Ser?Gln?Phe?Asn?Thr?Ala?Ser?Ala

835?????????????????840?????????????????845Gly?Tyr?Leu?Asn?Ala?Asn?Val?Arg?Tyr?Asn?Asn?Val?Gly?Thr?Gly?Ala

850?????????????????855?????????????????860Ile?Tyr?Asp?Val?Lys?Pro?Thr?Thr?Ser?Phe?Val?Leu?Asn?Asn?Asp?Thr865?????????????????870?????????????????875?????????????????880Ile?Ala?Thr?Ile?Thr?Ala?Lys?Ser?Asn?Ser?Thr?Ala?Leu?Asn?Ile?Ser

885??????????????????890?????????????????895Pro?Gly?Glu?Ser?Tyr?Pro?Lys?Lys?Gly?Gln?Asn?Gly?Ile?Ala?Ile?Thr

900?????????????????905?????????????????910

Ser?Met?Asp?Asp?Phe?Asn?Ser?His?Pro?Ile?Thr?Leu?Asn?Lys?Lys?Gln

915?????????????????920?????????????????925Val?Asp?Asn?Leu?Leu?Asn?Asn?Lys?Pro?Met?Met?Leu?Glu?Thr?Asn?Gln???930??????????????????935?????????????????940Thr?Asp?Gly?Val?Tyr?Lys?Ile?Lys?Asp?Thr?His?Gly?Asn?Ile?Val?Thr945?????????????????950?????????????????955?????????????????960Gly?Gly?Glu?Trp?Asn?Gly?Val?Ile?Gln?Gln?Ile?Lys?Ala?Lys?Thr?Ala

965?????????????????970?????????????????975Ser?Ile?Ile?Val?Asp?Asp?Gly?Glu?Arg?Val?Ala?Glu?Lys?Arg?Val?Ala

980?????????????????985?????????????????990Ala?Lys?Asp?Tyr?Glu?Asn?Pro?Glu?Asp?Lys?Thr?Pro?Ser?Leu?Thr?Leu

995?????????????????1000????????????????1005Lys?Asp?Ala?Leu?Lys?Leu?Ser?Tyr?Pro?Asp?Glu?Ile?Lys?Glu?Ile?Glu

1010????????????????1015????????????????1020Gly?Leu?Leu?Tyr?Tyr?Lys?Asn?Lys?Pro?Ile?Tyr?Glu?Ser?Ser?Val?Met1025????????????????1030????????????????1035????????????????1040Thr?Tyr?Leu?Asp?Glu?Asn?Thr?Ala?Lys?Glu?Val?Thr?Lys?Gln?Leu?Asn

1045????????????????1050????????????????1055Asp?Thr?Thr?Gly?Lys?Phe?Lys?Asp?Val?Ser?His?Leu?Tyr?Asp?Val?Lys

1060????????????????1065????????????????1070Leu?Thr?Pro?Lys?Met?Asn?Val?Thr?Ile?Lys?Leu?Ser?Ile?Leu?Tyr?Asp

107?????????????????1080????????????????1085Asn?Ala?Glu?Ser?Asn?Asp?Asn?Ser?Ile?Gly?Lys?Trp?Thr?Asn?Thr?Asn

1090????????????????1095????????????????1100Ile?Val?Ser?Gly?Gly?Asn?Asn?Gly?Lys?Lys?Gln?Tyr?Ser?Ser?Asn?Asn1105????????????????1110????????????????1115????????????????1120Pro?Asp?Ala?Asn?Leu?Thr?Leu?Asn?Thr?Asp?Ala?Gln?Glu?Lys?Leu?Asn

1125????????????????1130????????????????1135Lys?ASn?Arg?Asp?Tyr?Tyr?Ile?Ser?Leu?Tyr?Met?Lys?Ser?Glu?Lys?Asn

1140????????????????1145????????????????1150Thr?Gln?Cys?Glu?Ile?Thr?Ile?Asp?Gly?Glu?Ile?Tyr?Pro?Ile?Thr?Thr

1155????????????????1160????????????????1165Lys?Thr?Val?Asn?Val?Asn?Lys?Asp?Asn?Tyr?Lys?Arg?Leu?Asp?Ile?Ile

1170????????????????1175????????????????1180Ala?His?Asn?Ile?Lys?Ser?Asn?Pro?Ile?Ser?Ser?Leu?His?Ile?Lys?Thr1185????????????????1190????????????????1195????????????????1200Asn?Asp?Glu?Ile?Thr?Leu?Phe?Trp?Asp?Asp?Ile?Ser?Ile?Thr?Asp?Val

1205????????????????1210????????????????1215Ala?Ser?Ile?Lys?Pro?Glu?Asn?Leu?Thr?Asp?Ser?Glu?Ile?Lys?Gln?Ile

1220????????????????1225????????????????1230Tyr?Ser?Arg?Tyr?Gly?Ile?Lys?Leu?Glu?Asp?Gly?Ile?Leu?Ile?Asp?Lys

1235????????????????1240????????????????1245Lys?Gly?Gly?Ile?His?Tyr?Gly?Glu?Phe?Ile?Asn?Glu?Ala?Ser?Phe?Asn

1250????????????????1255???????????????1260Ile?Glu?Pro?Leu?Gln?Asn?Tyr?Val?Thr?Lys?Tyr?Glu?Val?Thr?Tyr?Ser1265????????????????1270????????????????1275????????????????1280Ser?Glu?Leu?Gly?Pro?Asn?Val?Ser?Asp?Thr?Leu?Glu?Ser?Asp?Lys?Ile

1285????????????????1290????????????????1295Tyr?Lys?Asp?Gly?Thr?Ile?Lys?Phe?Asp?Phe?Thr?Lys?Tyr?Ser?Lys?Ash

1300????????????????1305????????????????1310Glu?Gln?Gly?Leu?Phe?Tyr?Asp?Ser?Gly?Leu?Asn?Trp?Asp?Phe?Lys?Ile

1315????????????????1320????????????????1325Asn?Ala?Ile?Thr?Tyr?Asp?Gly?Lys?Glu?Met?Asn?Val?Phe?His?Arg?Tyr???1330?????????????????1335????????????????1340Asn?Lys1345

(2) about the information of SEQ ID NO:24

(ⅰ) sequence signature

(A) length: 1399 nucleotide

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: DNA (genome)

(ⅸ) feature:

(A) title/key: misc_feature

(B) position: 1..1386

(D) out of Memory :/remarks=" from the corn optimization dna sequence dna of the coding VIP2A (a) of AB78.”

( ⅹⅰ ) ：SEQ ID NO:24:ATGAAGCGCA TGGAGGGCAA GCTGTGCATG GTGAGCAAGA AGCTCCAGGT GGTGACCAAG 60ACCGTGCTGC TGAGCACCGT GTTCAGCATC AGCCTGCTGA ACAACGAGGT GATCAAGGCC 120GAGCAGCTGA ACATCAACAG CCAGAGCAAG TACACCAACC TCCAGAACCT GAAGATCACC 180GACAAGGTGG AGGACTTCAA GGAGGACAAG GAGAAGGCCA AGGAGTGGGG CAAGGAGAAG 240GAGAAGGAGT GGAAGCTTAC CGCTACCGAG AAGGGCAAGA TGAACAACTT CCTGGACAAC 300AAGAACGACA TCAAGACCAA CTACAAGGAG ATCACCTTCA GCATGGCCGG CAGCTTCGAG 360GACGAGATCA AGGACCTGAA GGAGATCGAC AAGATGTTCG ACAAGACCAA CCTGAGCAAC 420AGCATCATCA CCTACAAGAA CGTGGAGCCC ACCACCATCG GCTTCAACAA GAGCCTGACC 480GAGGGCAACA CCATCAACAG CGACGCCATG GCCCAGTTCA AGGAGCAGTT CCTGGACCGC 540GACATCAAGT TCGACAGCTA CCTGGACACC CACCTGACCG CCCAGCAGGT GAGCAGCAAG 600GAGCGCGTGA TCCTGAAGGT GACCGTCCCC AGCGGCAAGG GCAGCACCAC CCCCACCAAG 660GCCGGCGTGA TCCTGAACAA CAGCGAGTAC AAGATGCTGA TCCGCAACGG CTACATGGTG 720CACGTGGACA AGGTGAGCAA GGTGGTGAAG AAGGGCGTGG AGTGCCTCCA GATCGAGGGC 780ACCCTGAAGA AGAGTCTAGA CTTCAAGAAC GACATCAACG CCGAGGCCCA CAGCTGGGGC 840ATGAAGAACT ACGAGGAGTG GGCCAAGGAC CTGACCGACA GCCAGCGCGA GGCCCTGGAC 900GGCTACGCCC GCCAGGACTA CAAGGAGATC AACAACTACC TGCGCAACCA GGGCGGCAGC 960GGCAACGAGA AGCTGGACGC CCAGATCAAG AACATCAGCG ACGCCCTGGG CAAGAAGCCC 1020ATCCCCGAGA ACATCACCGT GTACCGCTGG TGCGGCATGC CCGAGTTCGG CTACCAGATC 1080AGCGACCCCC TGCCCAGCCT GAAGGACTTC GAGGAGCAGT TCCTGAACAC CATCAAGGAG 1140GACAAGGGCT ACATGAGCAC CAGCCTGAGC AGCGAGCGCC TGGCCGCCTT CGGCAGCCGC 1200AAGATCATCC TGCGCCTGCA GGTGCCCAAG GGCAGCACCG GCGCCTACCT GAGCGCCATC 1260GGCGGCTTCG CCAGCGAGAA GGAGATCCTG CTGGACAAGG ACAGCAAGTA CCACATCGAC 1320AAGGTGACCG AGGTGATCAT CAAGGGCGTG AAGCGCTACG TGGTGGACGC CACCCTGCTG 1380ACCAACTAGA TCTGAGCTC 1399

(2) about the information of SEQ ID NO:25

(ⅰ) sequence signature

(A) length: 19 amino acid

(B) type: amino acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: peptide

(ⅸ) feature:

(A) title/key: peptide

(B) position: 1..19

(D) out of Memory :/remarks=" from the secreting signal peptide of cell secreting outside VIP2 "

(ⅹ ⅰ) sequence description: SEQ ID NO:25:Gly Trp Ser Trp Ile Phe Leu Phe Leu Leu Ser Gly Ala Ala Gly Val1 5 10 15His Cys Leu

(2) about the information of SEQ ID NO:26

(ⅰ) sequence signature

(A) length: 2655 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: other nucleic acid

(A) describe :/desc=" synthetic DNA "

(ⅲ) the false plan: not

(ⅸ) feature:

(A) title/key: misc_feathure

(B) position: 1..2655

(D) out of Memory :/remarks=" the corn optimization dna sequence dna of coding VIP1A (a).”

( ⅹⅰ ) ：SEQ ID NO:26：ATGAAGAACA TGAAGAAGAA GCTGGCCAGC GTGGTGACCT GCACCCTGCT GGCCCCCATG 60TTCCTGAACG GCAACGTGAA CGCCGTGTAC GCCGACAGCA AGACCAACCA GATCAGCACC 120ACCCAGAAGA ACCAGCAGAA GGAGATGGAC GCGAAGGGCC TGCTGGGCTA CTACTTCAAG 180GGCAAGGACT TCAGCAACCT GACCATGTTC GCCCCCACGC GTGACAGCAC CCTGATCTAC 240GACCAGCAGA CCGCCAACAA GCTGCTGGAC AAGAAGCAGC AGGAGTACCA GAGCATCCGC 300TGGATCGGCC TGATCCAGAG CAAGGAGACC GGCGACTTCA CCTTCAACCT GAGCGAGGAC 360GAGCAGGCCA TCATCGAGAT CAACGGCAAG ATCATCAGCA ACAAGGGCAA GGAGAAGCAG 420GTGGTGCACC TGGAGAAGGG CAAGCTGGTG CCCATCAAGA TCGAGTACCA GAGCGACACC 480AAGTTCAACA TCGACAGCAA GACCTTCAAG GAGCTGAAGC TTTTCAAGAT CGACAGCCAG 540AACCAGCCCC AGCAGGTGCA GCAGGACGAG CTGCGCAACC CCGAGTTCAA CAAGAAGGAG 600AGCCAGGAGT TCCTGGCCAA GCCCAGCAAG ATCAACCTGT TCACCCAGCA GATGAAGCGC 660GAGATCGACG AGGACACCGA CACCGACGGC GACAGCATCC CCGACCTGTG GGAGGAGAAC 720GGCTACACCA TCCAGAACCG CATCGCCGTG AAGTGGGACG ACAGCCTGGC TAGCAAGGGC 780TACACCAAGT TCGTGAGCAA CCCCCTGGAG AGCCACACCG TGGGCCGACC CTACACCGAC 840TACGAGAAGG CCGCCCGCGA CCTGGACCTG AGCAACGCCA AGGAGACCTT CAACCCCCTG 900GTGGCCGCCT TCCCCAGCGT GAACGTGAGC ATGGAGAAGG TGATCCTGAG CCCCAACGAG 960AACCTGAGCA ACAGCGTGGA GAGCCCACTC AGCACCAACT GGAGCTACAC CAACACCGAG 1020GGCGCCAGCG TGGAGGCCGG CATCGGTCCC AAGGGCATCA GCTTCGGGGT GAGCGTGAAC 1080TACCAGCACA GCGAGACCGT GGCCCAGGAG TGGGGCACCA GCACCGGCAA CACCAGCCAG 1140TTCAACACCG CCAGCGCCGG CTACCTGAAC GCCAACGTGC GCTACAACAA CGTGGGCACC 1200GGCGCCATCT ACGACGTGAA GCCCACCACC AGCTTCGTGC TGAACAACGA CACCATCGCC 1260ACCATCACCG CCAAGTCGAA TTCCACCGCC CTGAACATCA GCCCCGGCGA GAGCTACCCC 1320AAGAAGGGCC AGAACGGCAT CGCCATCACC AGCATGGACG ACTTCAACAG CCACCCCATC 1380ACCCTGAACA AGAAGCAGGT GGACAACCTG CTGAACAACA AGCCCATGAT GCTGGAGACC 1440AACCAGACCG ACGGCGTCTA CAAGATCAAG GACACCCACG GCAACATCGT GACGGGCGGC 1500GAGTGGAACG GCGTGATCCA GCAGATCAAG GCCAAGACCG CCAGCATCAT CGTCGACGAC 1560GGCGAGCGCG TGGCCGAGAA GCGCGTGGCC GCCAAGGACT ACGAGAACCC CGAGGACAAG 1620ACCCCCAGCC TGACCCTGAA GGACGCCCTG AAGCTGAGCT ACCCCGACGA GATCAAGGAG 1680ATCGAGGGCT TGCTGTACTA CAAGAACAAG CCCATCTACG AGAGCAGCGT GATGACCTAT 1740CTAGACGAGA ACACCGCCAA GGAGGTGACC AAGCAGCTGA ACGACACCAC CGGCAAGTTC 1800AAGGACGTGA GCCACCTGTA CGACGTGAAG CTGACCCCCA AGATGAACGT GACCATCAAG 1860CTGAGCATCC TGTACGACAA CGCCGAGAGC AACGACAACA GCATCGGCAA GTGGACCAAC 1920ACCAACATCG TGAGCGGCGG CAACAACGGC AAGAAGCAGT ACAGCAGCAA CAACCCCGAC 1980GCCAACCTGA CCCTGAACAC CGACGCCCAG GAGAAGCTGA ACAAGAACCG CGACTACTAC 2040ATCAGCCTGT ACATGAAGAG CGAGAAGAAC ACCCAGTGCG AGATCACCAT CGACGGCGAG 2100ATATACCCCA TCACCACCAA GACCGTGAAC GTGAACAAGG ACAACTACAA GCGCCTGGAC 2160ATCATCGCCC ACAACATCAA GAGCAACCCC ATCAGCAGCC TGCACATCAA GACCAACGAC 2220GAGATCACCC TGTTCTGGGA CGACATATCG ATTACCGACG TCGCCAGCAT CAAGCCCGAG 2280AACCTGACCG ACAGCGAGAT CAAGCAGATA TACAGTCGCT ACGGCATCAA GCTGGAGGAC 2340GGCATCCTGA TCCGACAGAA AGGCGGCATC CACTACGGCG AGTTCATCAA CGAGGCCAGC 2400TTCAACATCG AGCCCCTGCA GAACTACGTG ACCAAGTACG AGGTGACCTA CAGCAGCGAG 2460CTGGGCCCCA ACGTGAGCGA CACCCTGGAG AGCGACAAGA TTTACAAGGA CGGCACCATC 2520AAGTTCGACT TCACCAAGTA CAGCAAGAAC GAGCAGGGCC TGTTCTACGA CAGCGGCCTG 2580AACTGGGACT TCAAGATCAA CGCCATCACC TACGACGGCA AGGAGATGAA CGTGTTCCAC 2640CGCTACAACA AGTAG 2655

(2) about the information of SEQ ID NO:27

(ⅰ) sequence signature

(A) length: 1389 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: other nucleic acid

(A) describe :/desc=" synthetic DNA "

(ⅲ) the false plan: not

(ⅸ) feature:

(A) title/key: misc_feathure

(B) position: 1..1389

(D) out of Memory :/remarks=" the corn optimization dna sequence dna of coding VIP2A (a).”

( ⅹⅰ ) ：SEQ ID NO:27：ATGAAGCGCA TGGAGGGCAA GCTGTTCATG GTGAGCAAGA AGCTCCAGGT GGTGACCAAG 60ACCGTGCTGC TGAGCACCGT GTTCAGCATC AGCCTGCTGA ACAACGAGGT GATCAAGGCC 120GAGCAGCTGA ACATCAACAG CCAGAGCAAG TACACCAACC TCCAGAACCT GAAGATCACC 180GACAAGGTGG AGGACTTCAA GGAGGACAAG GAGAAGGCCA AGGAGTGGGG CAAGGACAAG 240GAGAAGGAGT GGAAGCTTAC CGCCACCGAG AAGGGCAAGA TGAACAACTT CCTGGACAAC 300AAGAACGACA TCAAGACCAA CTACAAGGAG ATCAACCTCA GCATAGCCGG CAGCTTCGAG 360GACGAGATCA AGGACCTGAA GGAGATCGAC AAGATGTTCG ACAAGACCAA CCTGAGCAAC 420AGCATCATCA CCTACAAGAA CGTGGAGCCC ACCACCATCG GCTTCAACAA GAGCCTGACC 480GAGGGCAACA CCATCAACAG CGACGCCATG GCCCAGTTCA AGGAGCAGTT CCTGGACCGC 540GACATCAAGT TCGACAGCTA CCTGGACACC CACCGGACCG CCCAGCAGGT GAGCAGCAAG 600GAGCGCGTGA TCCTGAAGGT GACCGTCCCC AGCGGCAAGG GCAGCACCAC CCCCACCAAG 660GCCGGCGTGA TCCTGAACAA CAGCGAGTAC AAGATGCTGA TCGACAACGG CTACATGGTG 720CACGTGGACA AGGTGAGCAA GGTGGTGAAG AAGGGCGTGG AGTGCCTCCA GATCGAGGGC 780ACCCTGAAGA AGAGTCTAGA CTTCAAGAAC GACATCAACG CCGAGGCCCA CAGCTGGGGC 840ATGAAGAACT ACGAGGAGTG GGCCAAGGAC CTGACCGACA GCCAGCGCGA GGCCCTGGAC 900GGCTACGCCC GCCAGGACTA cAAGGAGATC AACAACTACC TGCGCAACCA GGGCGGCAGC 960GGCAACGAGA AGCTGGACGC CCAGATCAAG AACATCAGCG ACGCCCTGGG CAAGAAGCCC 1020ATCCCCGAGA ACATCACCGT GTACCGCTGG TGCGGCATGC CCGAGTTCGG CTACCAGATC 1080AGCGACCCCC TGCCCAGCCT GAAGGACTTC GAGGAGCAGT TCCTGAACAC CATCAAGGAG 1140GACAAGGGCT ACATGAGCAC CAGCCTGAGC AGCGAGCGCC TGGCCGCCTT CGGCAGCCGC 1200AAGATCATCC TGCGCCTGCA GGTGCCCAAG GGCAGCACTG GTGCCTACCT GAGCGCCATC 1260GGCGGCTTCG CCAGCGAGAA GGAGATCCTG CTGGATAAGG ACAGCAAGTA CCACATCGAC 1320AAGGTGACCG AGGTGATCAT CAAGGGCGTG AAGCGCTACG TGGTGGACGC CACCCTGCTG 1380ACCAACTAG 1389

(2) about the information of SEQ ID NO:28

(ⅰ) sequence signature

(A) length: 2378 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: DNA (genome)

(ⅲ) the false plan: not

(ⅸ) feature:

(A) title/key: CDS

(B) position: 9..2375

(D) out of Memory :/remarks=" natural DNA sequence from coding VIP3A (a) albumen of AB88 is contained among the pCIB7104.”

(ⅹ ⅰ) sequence description: SEQ ID NO:28:AGATGAAC ATG AAC AAG AAT AAT ACT AAA TTA AGC ACA AGA GCC TTA CCA 50

Met?Asn?Lys?Asn?Asn?Thr?Lys?Leu?Ser?Thr?Arg?Ala?Leu?Pro

1???????????????5??????????????????10AGT?TTT?ATT?GAT?TAT?TTT?AAT?GGC?ATT?TAT?GGA?TTT?GCC?ACT?GGT?ATC?????????98Ser?Phe?Ile?Asp?Tyr?Phe?Asn?Gly?Ile?Tyr?Gly?Phe?Ala?Thr?Gly?Ile?15??????????????????20??????????????????25??????????????????30AAA?GAC?ATT?ATG?AAC?ATG?ATT?TTT?AAA?ACG?GAT?ACA?GGT?GGT?GAT?CTA????????146Lys?Asp?Ile?Met?Asn?Met?Ile?Phe?Lys?Thr?Asp?Thr?Gly?Gly?Asp?Leu

35??????????????????40??????????????????45ACC?CTA?GAC?GAA?ATT?TTA?AAG?AAT?CAG?CAG?TTA?CTA?AAT?GAT?ATT?TCT????????194Thr?Leu?Asp?Glu?Ile?Leu?Lys?Asn?Gln?Gln?Leu?Leu?Asn?Asp?Ile?Ser

50??????????????????55??????????????????60GGT?AAA?TTG?GAT?GGG?GTG?AAT?GGA?AGC?TTA?AAT?GAT?CTT?ATC?GCA?CAG????????242Gly?Lys?Leu?Asp?Gly?Val?Asn?Gly?Ser?Leu?Asn?Asp?Leu?Ile?Ala?Gln

65??????????????????70??????????????????75GGA?AAC?TTA?AAT?ACA?GAA?TTA?TCT?AAG?GAA?ATA?TTA?AAA?ATT?GCA?AAT????????290Gly?Asn?Leu?Asn?Thr?Glu?Leu?Ser?Lys?Glu?Ile?Leu?Lys?Ile?Ala?Asn

80??????????????????85??????????????????90GAA?CAA?AAT?CAA?GTT?TTA?AAT?GAT?GTT?AAT?AAC?AAA?CTC?GAT?GCG?ATA????????338Glu?Gln?Asn?Gln?Val?Leu?Asn?Asp?Val?Asn?Asn?Lys?Leu?Asp?Ala?Ile?95?????????????????100?????????????????105?????????????????110AAT?ACG?ATG?CTT?CGG?GTA?TAT?CTA?CCT?AAA?ATT?ACC?TCT?ATG?TTG?AGT????????386Asn?Thr?Met?Leu?Arg?Val?Tyr?Leu?Pro?Lys?Ile?Thr?Ser?Met?Leu?Ser

115?????????????????120?????????????????125GAT?GTA?ATG?AAA?CAA?AAT?TAT?GCG?CTA?AGT?CTG?CAA?ATA?GAA?TAC?TTA????????434Asp?Val?Met?Lys?Gln?Asn?Tyr?Ala?Leu?Ser?Leu?GLn?Ile?Glu?Tyr?Leu

130?????????????????135?????????????????140AGT?AAA?CAA?TTG?CAA?GAG?ATT?TCT?GAT?AAG?TTG?GAT?ATT?ATT?AAT?GTA????????482Ser?Lys?Gln?Leu?Gln?Glu?Ile?Ser?Asp?Lys?Leu?Asp?Ile?Ile?Asn?Val

145?????????????????150?????????????????155AAT?GTA?CTT?ATT?AAC?TCT?ACA?CTT?ACT?GAA?ATT?ACA?CCT?GCG?TAT?CAA????????530Asn?Val?Leu?Ile?Asn?Ser?Thr?Leu?Thr?Glu?Ile?Thr?Pro?Ala?Tyr?Gln

160?????????????????165?????????????????170AGG?ATT?AAA?TAT?GTG?AAC?GAA?AAA?TTT?GAG?GAA?TTA?ACT?TTT?GCT?ACA????????578Arg?Ile?Lys?Tyr?Val?Asn?Glu?Lys?Phe?Glu?Glu?Leu?Thr?Phe?Ala?Thr175????????????????180??????????????????185?????????????????190GAA?ACT?AGT?TCA?AAA?GTA?AAA?AAG?GAT?GGC?TCT?CCT?GCA?GAT?ATT?CTT????????626Glu?Thr?Ser?Ser?Lys?Val?Lys?Lys?Asp?Gly?Ser?Pro?Ala?Asp?Ile?Leu

195?????????????????200?????????????????205GAT?GAG?TTA?ACT?GAG?TTA?ACT?GAA?CTA?GCG?AAA?AGT?GTA?ACA?AAA?AAT????????674Asp?Glu?Leu?Thr?Glu?Leu?Thr?Glu?Leu?Ala?Lys?Ser?Val?Thr?Lys?Asn

210??????????????????215????????????????220GAT?GTG?GAT?GGT?TTT?GAA?TTT?TAC?CTT?AAT?ACA?TTC?CAC?GAT?GTA?ATG????????722Asp?Val?Asp?Gly?Phe?Glu?Phe?Tyr?Leu?Asn?Thr?Phe?His?Asp?Val?Met

225??????????????????230????????????????235GTA?GGA?AAT?AAT?TTA?TTC?GGG?CGT?TCA?GCT?TTA?AAA?ACT?GCA?TCG?GAA????????770Val?Gly?Asn?Asn?Leu?Phe?Gly?Arg?Ser?Ala?Leu?Lys?Thr?Ala?Ser?Glu

240??????????????????245????????????????250TTA?ATT?ACT?AAA?GAA?AAT?GTG?AAA?ACA?AGT?GGC?AGT?GAG?GTC?GGA?AAT????????818Leu?Ile?Thr?Lys?Glu?Asn?Val?Lys?Thr?Ser?Gly?Ser?Glu?Val?Gly?Asn255?????????????????260?????????????????265?????????????????270GTT?TAT?AAC?TTC?TTA?ATT?GTA?TTA?ACA?GCT?CTG?CAA?GCC?CAA?GCT?TTT????????866Val?Tyr?Asn?Phe?Leu?Ile?Val?Leu?Thr?Ala?Leu?Gln?Ala?Gln?Ala?Phe

275?????????????????280?????????????????285CTT?ACT?TTA?ACA?ACA?TGC?CGA?AAA?TTA?TTA?GGC?TTA?CCA?GAT?ATT?GAT????????914Leu?Thr?Leu?Thr?Thr?Cys?Arg?Lys?Leu?Leu?Gly?Leu?Ala?Asp?Ile?Asp

290?????????????????295????????????????300TAT?ACT?TCT?ATT?ATG?AAT?GAA?CAT?TTA?AAT?AAG?GAA?AAA?GAG?GAA?TTT????????962Tyr?Thr?Ser?Ile?Met?Asn?Glu?His?Leu?Asn?Lys?Glu?Lys?Glu?Glu?Phe

305?????????????????310?????????????????315AGA?GTA?AAC?ATC?CTC?CCT?ACA?CTT?TCT?AAT?ACT?TTT?TCT?AAT?CCT?AAT????????1010Arg?Val?Asn?Ile?Leu?Pro?Thr?Leu?Ser?Asn?Thr?Phe?Ser?Asn?Pro?Asn

320?????????????????325?????????????????330TAT?GCA?AAA?GTT?AAA?GGA?AGT?GAT?GAA?GAT?GCA?AAG?ATG?ATT?GTG?GAA????????1058Tyr?Ala?Lys?Val?Lys?Gly?Ser?Asp?Glu?Asp?Ala?Lys?Met?Ile?Val?Glu335?????????????????340?????????????????345?????????????????350GCT?AAA?CCA?GGA?CAT?GCA?TTG?ATT?GGG?TTT?GAA?ATT?AGT?AAT?GAT?TCA????????1106Ala?Lys?Pro?Gly?His?Ala?Leu?Ile?Gly?Phe?Glu?Ile?Ser?Asn?Asp?Ser

355?????????????????360?????????????????365ATT?ACA?GTA?TTA?AAA?GTA?TAT?GAG?GCT?AAG?CTA?AAA?CAA?AAT?TAT?CAA????????1154Ile?Thr?Val?Leu?Lys?Val?Tyr?Glu?Ala?Lys?Leu?Lys?Gln?Asn?Tyr?Gln

370?????????????????375?????????????????380GTC?GAT?AAG?GAT?TCC?TTA?TCG?GAA?GTT?ATT?TAT?GGT?GAT?ATG?GAT?AAA????????1202Val?Asp?Lys?Asp?Ser?Leu?Ser?Glu?Val?Ile?Tyr?Gly?Asp?Met?Asp?Lys

385?????????????????390?????????????????395TTA?TTG?TGC?CCA?GAT?CAA?TCT?GAA?CAA?ATC?TAT?TAT?ACA?AAT?AAC?ATA????????1250Leu?Leu?Cys?Pro?Asp?Gln?Ser?Glu?Gln?Ile?Tyr?Tyr?Thr?Asn?Asn?Ile

400?????????????????405?????????????????410GTA?TTT?CCA?AAT?GAA?TAT?GTA?ATT?ACT?AAA?ATT?GAT?TTC?ACT?AAA?AAA????????1298Val?Phe?Pro?Asn?Glu?Tyr?Val?Ile?Thr?Lys?Ile?Asp?Phe?Thr?Lys?Lys415?????????????????420?????????????????425?????????????????430ATG?AAA?ACT?TTA?AGA?TAT?GAG?GTA?ACA?GCG?AAT?TTT?TAT?GAT?TCT?TCT????????1346Met?Lys?Thr?Leu?Arg?Tyr?Glu?Val?Thr?Ala?Asn?Phe?Tyr?Asp?Ser?Ser

435?????????????????440?????????????????445ACA?GGA?GAA?ATT?GAC?TTA?AAT?AAG?AAA?AAA?GTA?GAA?TCA?AGT?GAA?GCG????????1394Thr?Gly?Glu?Ile?Asp?Leu?Asn?Lys?Lys?Lys?Val?Glu?Ser?Ser?Glu?Ala

450?????????????????455?????????????????460GAG?TAT?AGA?ACG?TTA?AGT?GCT?AAT?GAT?GAT?GGG?GTG?TAT?ATG?CCG?TTA????????1442Glu?Tyr?Arg?Thr?Leu?Ser?Ala?Asn?Asp?Asp?Gly?Val?Tyr?Met?Pro?Leu

465?????????????????470?????????????????475GGT?GTC?ATC?AGT?GAA?ACA?TTT?TTG?ACT?CCG?ATT?AAT?GGG?TTT?GCC?CTC????????1490Gly?Val?Ile?Ser?Glu?Thr?Phe?Leu?Thr?Pro?Ile?Asn?Gly?Phe?Gly?Leu

480?????????????????485?????????????????490CAA?GCT?GAT?GAA?AAT?TCA?AGA?TTA?ATT?ACT?TTA?ACA?TGT?AAA?TCA?TAT????????1538Gln?Ala?Asp?Glu?Asn?Ser?Arg?Leu?Ile?Thr?Leu?Thr?Cys?Lys?Ser?Tyr495?????????????????500?????????????????505?????????????????510TTA?AGA?GAA?CTA?CTG?CTA?GCA?ACA?GAC?TTA?AGC?AAT?AAA?GAA?ACT?AAA????????1586Leu?Arg?Glu?Leu?Leu?Leu?Ala?Thr?Asp?Leu?Ser?Asn?Lys?Glu?Thr?Lys

515?????????????????520?????????????????525TTG?ATC?GTC?CCG?CCA?AGT?GGT?TTT?ATT?AGC?AAT?ATT?GTA?GAG?AAC?GGG???????1634Leu?Ile?Val?Pro?Pro?Ser?Gly?Phe?Ile?Ser?Asn?Ile?Val?Glu?Asn?Gly

530?????????????????535?????????????????540TCC?ATA?GAA?GAG?GAC?AAT?TTA?GAG?CCG?TGG?AAA?GCA?AAT?AAT?AAG?AAT???????1682Ser?Ile?Glu?Glu?Asp?Asn?Leu?Glu?Pro?Trp?Lys?Ala?Asn?Asn?Lys?Asn

545?????????????????550??????????????????555GCG?TAT?GTA?GAT?CAT?ACA?GGC?GGA?GTG?AAT?GGA?ACT?AAA?GCT?TTA?TAT???????1730Ala?Tyr?Val?Asp?His?Thr?Gly?Gly?Val?Asn?Gly?Thr?Lys?Ala?Leu?Tyr

560?????????????????565?????????????????570GTT?CAT?AAG?GAC?GGA?GGA?ATT?TCA?CAA?TTT?ATT?GGA?GAT?AAG?TTA?AAA???????1778Val?His?Lys?Asp?Gly?Gly?Ile?Ser?Gln?Phe?Ile?Gly?Asp?Lys?Leu?Lys575?????????????????580?????????????????585?????????????????590CCG?AAA?ACT?GAG?TAT?GTA?ATC?CAA?TAT?ACT?GTT?AAA?GGA?AAA?CCT?TCT???????1826Pro?Lys?Thr?Glu?Tyr?Val?Ile?Gln?Tyr?Thr?Val?Lys?Gly?Lys?Pro?Ser

595?????????????????600?????????????????605ATT?CAT?TTA?AAA?GAT?GAA?AAT?ACT?GGA?TAT?ATT?CAT?TAT?GAA?GAT?ACA???????1874Ile?His?Leu?Lys?Asp?Glu?Asn?Thr?Gly?Tyr?Ile?His?Tyr?Glu?Asp?Thr

610?????????????????615?????????????????620AAT?AAT?AAT?TTA?GAA?GAT?TAT?CAA?ACT?ATT?AAT?AAA?CGT?TTT?ACT?ACA???????1922Asn?Asn?Asn?Leu?Glu?Asp?Tyr?Gln?Thr?Ile?Asn?Lys?Arg?Phe?Thr?Thr

625?????????????????630?????????????????635GGA?ACT?GAT?TTA?AAG?GGA?GTG?TAT?TTA?ATT?TTA?AAA?AGT?CAA?AAT?GGA???????1970Gly?Thr?Asp?Leu?Lys?Gly?Val?Tyr?Leu?Ile?Leu?Lys?Ser?Gln?Asn?Gly

640?????????????????645?????????????????650GAT?GAA?GCT?TGG?GGA?GAT?AAC?TTT?ATT?ATT?TTG?GAA?ATT?AGT?CCT?TCT???????2018Asp?Glu?Ala?Trp?Gly?Asp?Asn?Phe?Ile?Ile?Leu?Glu?Ile?Ser?Pro?Ser655?????????????????660?????????????????665?????????????????670GAA?AAG?TTA?TTA?AGT?CCA?GAA?TTA?ATT?AAT?ACA?AAT?AAT?TGG?ACG?AGT???????2066Glu?Lys?Leu?Leu?Ser?Pro?Glu?Leu?Ile?Asn?Thr?Asn?Asn?Trp?Thr?Ser

675?????????????????680?????????????????685ACG?GGA?TCA?ACT?AAT?ATT?AGC?GGT?AAT?ACA?CTC?ACT?CTT?TAT?CAG?GGA???????2114Thr?Gly?Ser?Thr?Asn?Ile?Ser?Gly?Asn?Thr?Leu?Thr?Leu?Tyr?Gln?Gly

690?????????????????695?????????????????700GGA?CGA?GGG?ATT?CTA?AAA?CAA?AAC?CTT?CAA?TTA?GAT?AGT?TTT?TCA?ACT???????2162Gly?Arg?Gly?Ile?Leu?Lys?Gln?Asn?Leu?Gln?Leu?Asp?Ser?Phe?Ser?Thr

705?????????????????710?????????????????715TAT?AGA?GTG?TAT?TTT?TCT?GTG?TCC?GGA?GAT?GCT?AAT?GTA?AGG?ATT?AGA???????2210Tyr?Arg?Val?Tyr?Phe?Ser?Val?Ser?Gly?Asp?Ala?Asn?Val?Arg?Ile?Arg

720?????????????????725?????????????????730AAT?TCT?AGG?GAA?GTG?TTA?TTT?GAA?AAA?AGA?TAT?ATG?AGC?GGT?GCT?AAA???????2258Asn?Ser?Arg?Glu?Val?Leu?Phe?Glu?Lys?Arg?Tyr?Met?Ser?Gly?Ala?Lys735?????????????????740?????????????????745?????????????????750GAT?GTT?TCT?GAA?ATG?TTC?ACT?ACA?AAA?TTT?GAG?AAA?GAT?AAC?TTT?TAT???????2306Asp?Val?Ser?Glu?Met?Phe?Thr?Thr?Lys?Phe?Glu?Lys?Asp?Asn?Phe?Tyr

755?????????????????760?????????????????765ATA?GAG?CTT?TCT?CAA?GGG?AAT?AAT?TTA?TAT?GGT?GGT?CCT?ATT?GTA?CAT???????2354Ile?Glu?Leu?Ser?Gln?Gly?Asn?Asn?Leu?Tyr?Gly?Gly?Pro?Ile?Val?His

770?????????????????775?????????????????780TTT?TAC?GAT?GTC?TCT?ATT?AAG?TAA???????????????????????????????????????2378Phe?Tyr?Asp?Val?Ser?Ile?Lys

785

(2) about the information of SEQ ID NO:29

(ⅰ) sequence signature

(A) length: 789 amino acid

(B) type: amino acid

(D) topological structure: line style

(ⅰ) molecule type: protein

(ⅹ ⅰ) sequence description: SEQ ID NO:29:Met Asn Lys Asn Asn Thr Lys Leu Ser Thr Arg Ala Leu Pro Ser Phe 15 10 15Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys Asp

20??????????????????25??????????????????30Ile?Met?Asn?Met?Ile?Phe?Lys?Thr?Asp?Thr?Gly?Gly?Asp?Leu?Thr?Leu

35??????????????????40??????????????????45Asp?Glu?Ile?Leu?Lys?Asn?Gln?Gln?Leu?Leu?Asn?Asp?Ile?Ser?Gly?Lys

50??????????????????55??????????????????60Leu?Asp?Gly?Val?Asn?Gly?Ser?Leu?Asn?Asp?Leu?Ile?Ala?Gln?Gly?Asn?65??????????????????70??????????????????75??????????????????80Leu?Asn?Thr?Glu?Leu?Ser?Lys?Glu?Ile?Leu?Lys?Ile?Ala?Asn?Glu?Gln

85??????????????????90??????????????????95Asn?Gln?Val?Leu?Asn?Asp?Val?Asn?Asn?Lys?Leu?Asp?Ala?Ile?Asn?Thr

100?????????????????105?????????????????110Met?Leu?Arg?Val?Tyr?Leu?Pro?Lys?Ile?Thr?Ser?Met?Leu?Ser?Asp?Val

115?????????????????120?????????????????125Met?Lys?Gln?Asn?Tyr?Ala?Leu?Ser?Leu?Gln?Ile?Glu?Tyr?Leu?Ser?Lys

130?????????????????135?????????????????140Gln?Leu?Gln?Glu?Ile?Ser?Asp?Lys?Leu?Asp?Ile?Ile?Asn?Val?Asn?Val145?????????????????150?????????????????155?????????????????160Leu?Ile?Asn?Ser?Thr?Leu?Thr?Glu?Ile?Thr?Pro?Ala?Tyr?Gln?Arg?Ile

165?????????????????170?????????????????175Lys?Tyr?Val?Asn?Glu?Lys?Phe?Glu?Glu?Leu?Thr?Phe?Ala?Thr?Glu?Thr

180?????????????????185?????????????????190Ser?Ser?Lys?Val?Lys?Lys?Asp?Gly?Ser?Pro?Ala?Asp?Ile?Leu?Asp?Glu

195??????????????????200?????????????????205Leu?Thr?Glu?Leu?Thr?Glu?Leu?Ala?Lys?Ser?Val?Thr?Lys?Asn?Asp?Val

210?????????????????215?????????????????220Asp?Gly?Phe?Glu?Phe?Tyr?Leu?Asn?Thr?Phe?His?Asp?Val?Met?Val?Gly225?????????????????230?????????????????235?????????????????240Asn?Asn?Leu?Phe?Gly?Arg?Ser?Ala?Leu?Lys?Thr?Ala?Ser?Glu?Leu?Ile

245??????????????????250????????????????255Thr?Lys?Glu?Asn?Val?Lys?Thr?Ser?Gly?Ser?Glu?Val?Gly?Asn?Val?Tyr

260??????????????????265????????????????270Asn?Phe?Leu?Ile?Val?Leu?Thr?Ala?Leu?Gln?Ala?Gln?Ala?Phe?Leu?Thr

275??????????????????280????????????????285Leu?Thr?Thr?Cys?Arg?Lys?Leu?Leu?Gly?Leu?Ala?Asp?Ile?Asp?Tyr?Thr

290?????????????????295?????????????????300Ser?Ile?Met?Asn?Glu?His?Leu?Asn?Lys?Glu?Lys?Glu?Glu?Phe?Arg?Val305?????????????????310?????????????????315?????????????????320Asn?Ile?Leu?Pro?Thr?Leu?Ser?Asn?Thr?Phe?Ser?Asn?Pro?Asn?Tyr?Ala

325?????????????????330?????????????????335Lys?Val?Lys?Gly?Ser?Asp?Glu?Asp?Ala?Lys?Met?Ile?Val?Glu?Ala?Lys

340?????????????????345?????????????????350Pro?Gly?His?Ala?Leu?Ile?Gly?Phe?Glu?Ile?Ser?Asn?Asp?Ser?Ile?Thr

355?????????????????360?????????????????365Val?Leu?Lys?Val?Tyr?Glu?Ala?Lys?Leu?Lys?Gln?Asn?Tyr?Gln?Val?Asp

370?????????????????375?????????????????380Lys?Asp?Ser?Leu?Ser?Glu?Val?Ile?Tyr?Gly?Asp?Met?Asp?Lys?Leu?Leu385?????????????????390????????????????395??????????????????400Cys?Pro?Asp?Gln?Ser?Glu?Gln?Ile?Tyr?Tyr?Thr?Asn?Asn?Ile?Val?Phe

405?????????????????410?????????????????415Pro?Asn?Glu?Tyr?Val?Ile?Thr?Lys?Ile?Asp?Phe?Thr?Lys?Lys?Met?Lys

420?????????????????425?????????????????430Thr?Leu?Arg?Tyr?Glu?Val?Thr?Ala?Asn?Phe?Tyr?Asp?Ser?Ser?Thr?Gly

435?????????????????440?????????????????445Glu?Ile?Asp?Leu?Asn?Lys?Lys?Lys?Val?Glu?Ser?Ser?Glu?Ala?Glu?Tyr

450?????????????????455?????????????????460Arg?Thr?Leu?Ser?Ala?Asn?Asp?Asp?Gly?Val?Tyr?Met?Pro?Leu?Gly?Val465?????????????????470?????????????????475?????????????????480Ile?Ser?Glu?Thr?Phe?Leu?Thr?Pro?Ile?Asn?Gly?Phe?Gly?Leu?Gln?Ala

485?????????????????490?????????????????495Asp?Glu?Asn?Ser?Arg?Leu?Ile?Thr?Leu?Thr?Cys?Lys?Ser?Tyr?Leu?Arg

500?????????????????505?????????????????510Glu?Leu?Leu?Leu?Ala?Thr?Asp?Leu?Ser?Asn?Lys?Glu?Thr?Lys?Leu?Ile

515?????????????????520?????????????????525Val?Pro?Pro?Ser?Gly?Phe?Ile?Ser?Asn?Ile?Val?Glu?Asn?Gly?Ser?Ile

530?????????????????535?????????????????540Glu?Glu?Asp?Asn?Leu?Glu?Pro?Trp?Lys?Ala?Asn?Asn?Lys?Asn?Ala?Tyr545?????????????????550?????????????????555?????????????????560Val?Asp?His?Thr?Gly?Gly?Val?Asn?Gly?Thr?Lys?Ala?Leu?Tyr?Val?His

565?????????????????570?????????????????575Lys?Asp?Gly?Gly?Ile?Ser?Gln?Phe?Ile?Gly?Asp?Lys?Leu?Lys?Pro?Lys

580?????????????????585?????????????????590Thr?Glu?Tyr?Val?Ile?Gln?Tyr?Thr?Val?Lys?Gly?Lys?Pro?Ser?Ile?His

595?????????????????600?????????????????605Leu?Lys?Asp?Glu?Asn?Thr?Gly?Tyr?Ile?His?Tyr?Glu?Asp?Thr?Asn?Asn

610?????????????????615?????????????????620Asn?Leu?Glu?Asp?Tyr?Gln?Thr?Ile?Asn?Lys?Arg?Phe?Thr?Thr?Gly?Thr625?????????????????630?????????????????635?????????????????640Asp?Leu?Lys?Gly?Val?Tyr?Leu?Ile?Leu?Lys?Ser?Gln?Asn?Gly?Asp?Glu

645??????????????????650????????????????655Ala?Trp?Gly?Asp?Asn?Phe?Ile?Ile?Leu?Glu?Ile?Ser?Pro?Ser?Glu?Lys

660??????????????????665????????????????670Leu?Leu?Ser?Pro?Glu?Leu?Ile?Asn?Thr?Asn?Asn?Trp?Thr?Ser?Thr?Gly

675?????????????????680?????????????????685Ser?Thr?Asn?Ile?Ser?Gly?Asn?Thr?Leu?Thr?Leu?Tyr?Gln?Gly?Gly?Arg

690?????????????????695?????????????????700Gly?Ile?Leu?Lys?Gln?Asn?Leu?Gln?Leu?Asp?Ser?Phe?Ser?Thr?Tyr?Arg705?????????????????710?????????????????715?????????????????720Val?Tyr?Phe?Ser?Val?Ser?Gly?Asp?Ala?Asn?Val?Arg?Ile?Arg?Asn?Ser

725?????????????????730?????????????????735Arg?Glu?Val?Leu?Phe?Glu?Lys?Arg?Tyr?Met?Ser?Gly?Ala?Lys?Asp?Val

740?????????????????745?????????????????750Ser?Glu?Met?Phe?Thr?Thr?Lys?Phe?Glu?Lys?Asp?Asn?Phe?Tyr?Ile?Glu

755?????????????????760?????????????????765Leu?Ser?Gln?Gly?Asn?Asn?Leu?Tyr?Gly?Gly?Pro?Ile?Val?His?Phe?Tyr???770??????????????????775?????????????????780Asp?Val?Ser?Ile?Lys785

(2) about the information of SEQ ID NO:30

(ⅰ) sequence signature

(A) length: 2403 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: other nucleic acid

(A) describe :/desc=" synthetic DNA "

(ⅲ) the false plan: not

(ⅸ) feature:

(A) title/key: misc_feature

(B) position: 11..2389

(D) out of Memory :/remarks=" the corn optimization dna sequence dna of coding VIP3A (a).”

( ⅹⅰ ) ：SEQ ID NO:30:GGATCCACCA ATGAACATGA ACAAGAACAA CACCAAGCTG AGCACCCGCG CCCTGCCGAG 60CTTCATCGAC TACTTCAACG GCATCTACGG CTTCGCCACC GGCATCAAGG ACATCATGAA 120CATGATCTTC AAGACCGACA CCGGCGGCGA CCTGACCCTG GACGAGATCC TGAAGAACCA 180GCAGCTGCTG AACGACATCA GCGGCAAGCT GGACGGCGTG AACGGCAGCC TGAACGACCT 240GATCGCCCAG GGCAACCTGA ACACCGAGCT GAGCAAGGAG ATCCTTAAGA TCGCCAACGA 300GCAGAACCAG GTGCTGAACG ACGTGAACAA CAAGCTGGAC GCCATCAACA CCATGCTGCG 360CGTGTACCTG CCGAAGATCA CCAGCATGCT GAGCGACGTG ATGAAGCAGA ACTACGCCCT 420GAGCCTGCAG ATCGAGTACC TGAGCAAGCA GCTGCAGGAG ATCAGCGACA AGCTGGACAT 480CATCAACGTG AACGTCCTGA TCAACAGCAC CCTGACCGAG ATCACCCCCG CCTACCAGCG 540CATCGAAGTAC GTGAACGAGA AGTTCGAAGA GCTGACCTTC GCCACCGAGA CCAGCAGCAA 600GGTGAAGAAG GACGGCAGCC CGGCCGACAT CCTGGACGAG CTGACCGAGC TGACCGAGCT 660GGCCAAGAGC GTGACCAAGA ACGACGTGGA CGGCTTCGAG TTCTACCTGA ACACCTTCCA 720CGACGTGATG GTGGGCAACA ACCTGTTCGG CCGCAGCGCC CTGAAGACCG CCAGCGAGCT 780GATCACCAAG GAGAACGTGA AGACCAGCGG CAGCGAGGTG GGCAACGTGT ACAACTTCCT 840GATCGTGCTG ACCGCCCTGC AGGCCCAGGC CTTCCTGACC CTGACCACCT GTCGCAAGCT 900GCTGGGCCTG GCCGACATCG ACTACACCAG CATCATGAAC GAGCACTTGA ACAAGGAGAA 960GGAGGAGTTC CGCGTGAACA TCCTGCCGAC CCTGAGCAAC ACCTTCAGCA ACCCGAACTA 1020CGCCAAGGTG AAGGGCAGCG ACGAGGACGC CAAGATGATC GTGGAGGCTA AGCCGGGCCA 1080CGCGTTGATC GGCTTCGAGA TCAGCAACGA CAGCATCACC GTGCTGAAGG TGTACGAGGC 1140CAAGCTGAAG CAGAACTACC AGGTGGACAA GGACAGCTTG AGCGAGGTGA TCTACGGCGA 1200CATGGACAAG CTGCTGTGTC CGGACCAGAG CGAGCAAATC TACTACACCA ACAACATCGT 1260GTTCCCGAAC GAGTACGTGA TCACCAAGAT CGACTTCACC AAGAAGATGA AGACCCTGCG 1320CTACGAGGTG ACCGCCAACT TCTACGACAG CAGCACCGGC GAGATCGACC TGAACAAGAA 1380GAAGGTGGAG AGCAGCGAGG CCGAGTACCG CACCCTGAGC GCGAACGACG ACGGCGTCTA 1440CATGCCACTG GGCGTGATCA GCGAGACCTT CCTGACCCCG ATCAACGGCT TTGGCCTGCA 1500GGCCGACGAG AACAGCCGCC TGATCACCCT GACCTGTAAG AGCTACCTGC GCGAGCTGCT 1560GCTAGCCACC GACCTGAGCA ACAAGGAGAC CAAGCTGATC GTGCCACCGA GCGGCTTCAT 1620CAGCAACATC GTGGAGAACG GCAGCATCGA GGAGGACAAC CTGGAGCCGT GGAAGGCCAA 1680CAACAAGAAC GCCTACGTGG ACCACACCGG CGGCGTGAAC GGCACCAAGG CCCTGTACGT 1740GCACAAGGAC GGCGGCATCA GCCAGTTCAT CGGCGACAAG CTGAAGCCGA AGACCGAGTA 1800CGTGATCCAG TACACCGTGA AGGGCAAGCC ATCGATTCAC CTGAAGGACG AGAACACCGG 1860CTACATCCAC TACGAGGACA CCAACAACAA CCTGGAGGAC TACCAGACCA TCAACAAGCG 1920CTTCACCACC GGCACCGACC TGAAGGGCGT GTACCTGATC CTGAAGAGCC AGAACGGCGA 1980CGAGGCCTGG GGCGACAACT TCATCATCCT GGAGATCAGC CCGAGCGAGA AGCTGCTGAG 2040CCCGGAGCTG ATCAACACCA ACAACTGGAC CAGCACCGGC AGCACCAACA TCAGCGGCAA 2100CACCCTGACC CTGTACCAGG GCGGCCGCGG CATCCTGAAG CAGAACCTGC AGCTGGACAG 2160CTTCAGCACC TACCGCGTGT ACTTCAGCGT GAGCGGCGAC GCCAACGTGC GCATCCGCAA 2220CAGCCGCGAG GTGCTGTTCG AGAAGAGGTA CATGAGCGGC GCCAAGGACG TGAGCGAGAT 2280GTTCACCACC AAGTTCGAGA AGGACAACTT CTACATCGAG CTGAGCCAGG GCAACAACCT 2340GTACGGCGGC CCGATCGTGC ACTTCTACGA CGTGAGCATC AAGTTAACGT AGAGCTCAGA 2400TCT 2403

(2) about the information of SEQ ID NO:31

(ⅰ) sequence signature

(A) length: 2612 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: DNA (genome)

(ⅲ) the false plan: not

(ⅸ) feature:

(A) title/key: CDS

(B) position: 118..2484

(D) out of Memory :/remarks=" from the natural DNA sequence of the coding VIP3A (b) of AB424.”

(ⅹ ⅰ) sequence description: SEQ ID NO:31:ATTGAAATTG ATAAAAAGTT ATGAGTGTTT AATAATCAGT AATTACCAAT AAAGAATTAA 60GAATACAAGT TTACAAGAAA TAAGTGTTAC AAAAAATAGC TGAAAAGGAA GATGAAC 117ATG AAC AAG AAT AAT ACT AAA TTA AGC ACA AGA GCC TTA CCA AGT TTT 165Met Asn Lys Asn Asn Thr Lys Leu Ser Thr Arg Ala Leu Pro Ser Phe790 795 800 805ATT GAT TAT TTC AAT GGC ATT TAT GGA TTT GCC ACT GGT ATC AAA GAC 213Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys Asp

810?????????????????815?????????????????820ATT?ATG?AAC?ATG?ATT?TTT?AAA?ACG?GAT?ACA?GGT?GGT?GAT?CTA?ACC?CTA?????????261Ile?Met?Asn?Met?Ile?Phe?Lys?Thr?Asp?Thr?Gly?Gly?Asp?Leu?Thr?Leu

825?????????????????830?????????????????835GAC?GAA?ATT?TTA?AAG?AAT?CAG?CAG?CTA?CTA?AAT?GAT?ATT?TCT?GGT?AAA?????????309Asp?Glu?Ile?Leu?Lys?Asn?Gln?Gln?Leu?Leu?Asn?Asp?Ile?Ser?Gly?Lys

840?????????????????845?????????????????850TTG?GAT?GGG?GTG?AAT?GGA?AGC?TTA?AAT?GAT?CTT?ATC?GCA?CAG?GGA?AAC?????????357Leu?Asp?Gly?Val?Asn?Gly?Ser?Leu?Asn?Asp?Leu?Ile?Ala?Gln?Gly?Asn

855?????????????????860?????????????????865TTA?AAT?ACA?GAA?TTA?TCT?AAG?GAA?ATA?TTA?AAA?ATT?GCA?AAT?GAA?CAA????????405Leu?Asn?Thr?Glu?Leu?Ser?Lys?Glu?Ile?Leu?Lys?Ile?Ala?Asn?Glu?Gln870?????????????????875?????????????????880?????????????????885AAT?CAA?GTT?TTA?AAT?GAT?GTT?AAT?AAC?AAA?CTC?GAT?GCG?ATA?AAT?ACG????????453Asn?Gln?Val?Leu?Asn?Asp?Val?Asn?Asn?Lys?Leu?Asp?Ala?Ile?Asn?Thr

890?????????????????895?????????????????900ATG?CTT?CGG?GTA?TAT?CTA?CCT?AAA?ATT?ACC?TCT?ATG?TTG?AGT?GAT?GTA????????501Met?Leu?Arg?Val?Tyr?Leu?Pro?Lys?Ile?Thr?Ser?Met?Leu?Ser?Asp?Val

905?????????????????910?????????????????915ATG?AAA?CAA?AAT?TAT?GCG?CTA?AGT?CTG?CAA?ATA?GAA?TAC?TTA?AGT?AAA????????549Met?Lys?Gln?Asn?Tyr?Ala?Leu?Ser?Leu?Gln?Ile?Glu?Tyr?Leu?Ser?Lys

920?????????????????925?????????????????930CAA?TTG?CAA?GAG?ATT?TCT?GAT?AAG?TTG?GAT?ATT?ATT?AAT?GTA?AAT?GTA????????597Gln?Leu?Gln?Glu?Ile?Ser?Asp?Lys?Leu?Asp?Ile?Ile?Asn?Val?Asn?Val

935?????????????????940?????????????????945CTT?ATT?AAC?TCT?ACA?CTT?ACT?GAA?ATT?ACA?CCT?GCG?TAT?CAA?AGG?ATT????????645Leu?Ile?Asn?Ser?Thr?Leu?Thr?Glu?Ile?Thr?Pro?Ala?Tyr?Gln?Arg?Ile950?????????????????955?????????????????960?????????????????965AAA?TAT?GTG?AAC?GAA?AAA?TTT?GAG?GAA?TTA?ACT?TTT?GCT?ACA?GAA?ACT????????693Lys?Tyr?Val?Asn?Glu?Lys?Phe?Glu?Glu?Leu?Thr?Phe?Ala?Thr?Glu?Thr

970?????????????????975?????????????????980AGT?TCA?AAA?GTA?AAA?AAG?GAT?GGC?TCT?CCT?GCA?GAT?ATT?CGT?GAT?GAG????????741Ser?Ser?Lys?Val?Lys?Lys?Asp?Gly?Ser?Pro?Ala?Asp?Ile?Arg?Asp?Glu

985?????????????????990?????????????????995TTA?ACT?GAG?TTA?ACT?GAA?CTA?GCG?AAA?AGT?GTA?ACA?AAA?AAT?GAT?GTG????????789Leu?Thr?Glu?Leu?Thr?Glu?Leu?Ala?Lys?Ser?Val?Thr?Lys?Asn?Asp?Val

1000????????????????1005????????????????1010GAT?GGT?TTT?GAA?TTT?TAC?CTT?AAT?ACA?TTC?CAC?GAT?GTA?ATG?GTA?GGA????????837Asp?Gly?Phe?Glu?Phe?Tyr?Leu?Asn?Thr?Phe?His?Asp?Val?Met?Val?Gly

1015????????????????1020????????????????1025AAT?AAT?TTA?TTC?GGG?CGT?TCA?GCT?TTA?AAA?ACT?GCA?TCG?GAA?TTA?ATT????????885Asn?Asn?Leu?Phe?Gly?Arg?Ser?Ala?Leu?Lys?Thr?Ala?Ser?Glu?Leu?Ile1030????????????????1035????????????????1040????????????????1045ACT?AAA?GAA?AAT?GTG?AAA?ACA?AGT?GGC?AGT?GAG?GTC?GGA?AAT?GTT?TAT????????933Thr?Lys?Glu?Asn?Val?Lys?Thr?Ser?Gly?Ser?Glu?Val?Gly?Asn?Val?Tyr

1050????????????????1055????????????????1060AAC?TTC?CTA?ATT?GTA?TTA?ACA?GCT?CTG?CAA?GCA?AAA?GCT?TTT?CTT?ACT????????981Asn?Phe?Leu?Ile?Val?Leu?Thr?Ala?Leu?Gln?Ala?Lys?Ala?Phe?Leu?Thr

1065????????????????1070????????????????1075TTA?ACA?CCA?TGC?CGA?AAA?TTA?TTA?GGC?TTA?GCA?GAT?ATT?GAT?TAT?ACT????????1029Leu?Thr?Pro?Cys?Arg?Lys?Leu?Leu?Gly?Leu?Ala?Asp?Ile?Asp?Tyr?Thr

1080????????????????1085????????????????1090TCT?ATT?ATG?AAT?GAA?CAT?TTA?AAT?AAG?GAA?AAA?GAG?GAA?TTT?AGA?GTA???????1077Ser?Ile?Met?Asn?Glu?His?Leu?Asn?Lys?Glu?Lys?Glu?Glu?Phe?Arg?Val

1095????????????????1100????????????????1105AAC?ATC?CTC?CCT?ACA?CTT?TCT?AAT?ACT?TTT?TCT?AAT?CCT?AAT?TAT?GCA???????1125Asn?Ile?Leu?Pro?Thr?Leu?Ser?Asn?Thr?Phe?Ser?Asn?Pro?Asn?Tyr?Ala1110????????????????1115????????????????1120????????????????1125AAA?GTT?AAA?GGA?AGT?GAT?GAA?GAT?GCA?AAG?ATG?ATT?GTG?GAA?GCT?AAA???????1173Lys?Val?Lys?Gly?Ser?Asp?Glu?Asp?Ala?Lys?Met?Ile?Val?Glu?Ala?Lys

1130????????????????1135????????????????1140CCA?GGA?CAT?GCA?TTG?ATT?GGG?TTT?GAA?ATT?AGT?AAT?GAT?TCA?ATT?ACA???????1221Pro?Gly?His?Ala?Leu?Ile?Gly?Phe?Glu?Ile?Ser?Asn?Asp?Ser?Ile?Thr

1145????????????????1150????????????????1155GTA?TTA?AAA?GTA?TAT?GAG?GCT?AAG?CTA?AAA?CAA?AAT?TAT?CAA?GTC?GAT???????1269Val?Leu?Lys?Val?Tyr?Glu?Ala?Lys?Leu?Lys?Gln?Asn?Tyr?Gln?Val?Asp

1160?????????????????1165???????????????1170AAG?GAT?TCC?TTA?TCG?GAA?GTT?ATT?TAT?GGC?GAT?ATG?GAT?AAA?TTA?TTG???????1317Lys?Asp?Ser?Leu?Ser?Glu?Val?Ile?Tyr?Gly?Asp?Met?Asp?Lys?Leu?Leu

1175????????????????1180????????????????1185TGC?CCA?GAT?CAA?TCT?GGA?CAA?ATC?TAT?TAT?ACA?AAT?AAC?ATA?GTA?TTT???????1365Cys?Pro?Asp?Gln?Ser?Gly?Gln?Ile?Tyr?Tyr?Thr?Asn?Asn?Ile?Val?Phe1190????????????????1195????????????????1200????????????????1205CCA?AAT?GAA?TAT?GTA?ATT?ACT?AAA?ATT?GAT?TTC?ACT?AAA?AAA?ATG?AAA???????1413Pro?Asn?Glu?Tyr?Val?Ile?Thr?Lys?Ile?Asp?Phe?Thr?Lys?Lys?Met?Lys

1210????????????????1215????????????????1220ACT?TTA?AGA?TAT?GAG?GTA?ACA?GCG?AAT?TTT?TAT?GAT?TCT?TCT?ACA?GGA???????1461Thr?Leu?Arg?Tyr?Glu?Val?Thr?Ala?Asn?Phe?Tyr?Asp?Ser?Ser?Thr?Gly

1225????????????????1230????????????????1235

GAA?ATT?GAC?TTA?AAT?AAG?AAA?AAA?GTA?GAA?TCA?AGT?GAA?GCG?GAG?TAT???????1509Glu?Ile?Asp?Leu?Asn?Lys?Lys?Lys?Val?Glu?Ser?Ser?Glu?Ala?Glu?Tyr

1240????????????????1245????????????????1250AGA?ACG?TTA?AGT?GCT?AAT?GAT?GAT?GGG?GTG?TAT?ATG?CCG?TTA?GGT?GTC???????1557Arg?Thr?Leu?Ser?Ala?Asn?Asp?Asp?Gly?Val?Tyr?Met?Pro?Leu?Gly?Val

1255????????????????1260????????????????1265ATC?AGT?GAA?ACA?TTT?TTG?ACT?CCG?ATT?AAT?GGG?TTT?GGC?CTC?CAA?GCT???????1605Ile?Ser?Glu?Thr?Phe?Leu?Thr?Pro?Ile?Asn?Gly?Phe?Gly?Leu?Gln?Ala1270????????????????1275????????????????1280????????????????1285GAT?GAA?AAT?TCA?AGA?TTA?ATT?ACT?TTA?ACA?TGT?AAA?TCA?TAT?TTA?AGA???????1653Asp?Glu?Asn?Ser?Arg?Leu?Ile?Thr?Leu?Thr?Cys?Lys?Ser?Tyr?Leu?Arg

1290????????????????1295????????????????1300GAA?CTA?CTG?CTA?GCA?ACA?GAC?TTA?AGC?AAT?AAA?GAA?ACT?AAA?TTG?ATC???????1701Glu?Leu?Leu?Leu?Ala?Thr?Asp?Leu?Ser?Asn?Lys?Glu?Thr?Lys?Leu?Ile

1305?????????????????1310????????????????1315GTC?CCG?CCA?AGT?GGT?TTT?ATT?AGC?AAT?ATT?GTA?GAG?AAC?GGG?TCC?ATA???????1749Val?Pro?Pro?Ser?Gly?Phe?Ile?Ser?Asn?Ile?Val?Glu?Asn?Gly?Ser?Ile

1320????????????????1325????????????????1330GAA?GAG?GAC?AAT?TTA?GAG?CCG?TGG?AAA?GCA?AAT?AAT?AAG?AAT?GCG?TAT???????1797Glu?Glu?Asp?Asn?Leu?Glu?Pro?Trp?Lys?Ala?Asn?Asn?Lys?Asn?Ala?Tyr

1335????????????????1340????????????????1345GTA?GAT?CAT?ACA?GGC?GGA?GTG?AAT?GGA?ACT?AAA?GCT?TTA?TAT?GTT?CAT???????1845Val?Asp?His?Thr?Gly?Gly?Val?Asn?Gly?Thr?Lys?Ala?Leu?Tyr?Val?His1350????????????????1355????????????????1360????????????????1365AAG?GAC?GGA?GGA?ATT?TCA?CAA?TTT?ATT?GGA?GAT?AAG?TTA?AAA?CCG?AAA???????1893Lys?Asp?Gly?Gly?Ile?Ser?Gln?Phe?Ile?Gly?Asp?Lys?Leu?Lys?Pro?Lys

1370??????????????????1375??????????????1380ACT?GAG?TAT?GTA?ATC?CAA?TAT?ACT?GTT?AAA?GGA?AAA?CCT?TCT?ATT?CAT???????1941Thr?Glu?Tyr?Val?Ile?Gln?Tyr?Thr?Val?Lys?Gly?Lys?Pro?Ser?Ile?His

1385????????????????1390????????????????1395TTA?AAA?GAT?GAA?AAT?ACT?GGA?TAT?ATT?CAT?TAT?GAA?GAT?ACA?AAT?AAT???????1989Leu?Lys?Asp?Glu?Asn?Thr?Gly?Tyr?Ile?His?Tyr?Glu?Asp?Thr?Asn?Asn

1400????????????????1405????????????????1410AAT?TTA?GAA?GAT?TAT?CAA?ACT?ATT?AAT?AAA?CGT?TTT?ACT?ACA?GGA?ACT???????2037Asn?Leu?Glu?Asp?Tyr?Gln?Thr?Ile?Asn?Lys?Arg?Phe?Thr?Thr?Gly?Thr

1415????????????????1420????????????????1425GAT?TTA?AAG?GGA?GTG?TAT?TTA?ATT?TTA?AAA?AGT?CAA?AAT?GGA?GAT?GAA???????2085Asp?Leu?Lys?Gly?Val?Tyr?Leu?Ile?Leu?Lys?Ser?Gln?Asn?Gly?Asp?Glu1430????????????????1435????????????????1440????????????????1445GCT?TGG?GGA?GAT?AAC?TTT?ATT?ATT?TTG?GAA?ATT?AGT?CCT?TCT?GAA?AAG???????2133Ala?Trp?Gly?Asp?Asn?Phe?Ile?Ile?Leu?Glu?Ile?Ser?Pro?Ser?Glu?Lys

1450????????????????1455????????????????????1460TTA?TTA?AGT?CCA?GAA?TTA?ATT?AAT?ACA?AAT?AAT?TGG?ACG?AGT?ACG?GGA???????2181Leu?Leu?Ser?Pro?Glu?Leu?Ile?Asn?Thr?Asn?Asn?Trp?Thr?Ser?Thr?Gly

1465????????????????1470????????????????????1475TCA?ACT?AAT?ATT?AGC?GGT?AAT?ACA?CTC?ACT?CTT?TAT?CAG?GGA?GGA?CGA???????2229Ser?Thr?Asn?Ile?Ser?Gly?Asn?Thr?Leu?Thr?Leu?Tyr?Gln?Gly?Gly?Arg

1480????????????????1485????????????????????1490GGG?ATT?CTA?AAA?CAA?AAC?CTT?CAA?TTA?GAT?AGT?TTT?TCA?ACT?TAT?AGA???????2277Gly?Ile?Leu?Lys?Gln?Asn?Leu?Gln?Leu?Asp?Ser?Phe?Ser?Thr?Tyr?Arg

1495????????????????1500????????????????????1505GTG?TAT?TTC?TCT?GTG?TCC?GGA?GAT?GCT?AAT?GTA?AGG?ATT?AGA?AAT?TCT???????2325Val?Tyr?Phe?Ser?Val?Ser?Gly?Asp?Ala?Asn?Val?Arg?Ile?Arg?Asn?Ser1510????????????????1515???????????????????1520?????????????1525AGG?GAA?GTG?TTA?TTT?GAA?AAA?AGA?TAT?ATG?AGC?GGT?GCT?AAA?GAT?GTT???????2373Arg?Glu?Val?Leu?Phe?Glu?Lys?Arg?Tyr?Met?Ser?Gly?Ala?Lys?Asp?Val

1530????????????????1535????????????????1540TCT?GAA?ATG?TTC?ACT?ACA?AAA?TTT?GAG?AAA?GAT?AAC?TTC?TAT?ATA?GAG??????2421Ser?Glu?Met?Phe?Thr?Thr?Lys?Phe?Glu?Lys?Asp?Asn?Phe?Tyr?Ile?Glu

1545????????????????1550????????????????1555CTT?TCT?CAA?GGG?AAT?AAT?TTA?TAT?GGT?GGT?CCT?ATT?GTA?CAT?TTT?TAC??????2469Leu?Ser?Gln?Gly?Asn?Asn?Leu?Tyr?Gly?Gly?Pro?Ile?Val?His?Phe?Tyr

1560?????????????????1565?????????????????1570GAT?GTC?TCT?ATT?AAG?TAAGATCGGG?ATCTAATATT?AACAGTTTTT?AGAAGCTAAT??????2524Asp?Val?Ser?Ile?Lys

1575TCPTGTATAA?TGTCCTTGAT?TATGGAAAAA?CACAATTTTG?TTTGCTAAGA?TGTATATATA????2584GCTCACTCAT?TAAAAGGCAA?TCAAGCTT??????????????????????????????????????????????????????2612

(2) about the information of SEQ ID NO:32

(ⅰ) sequence signature

(A) length: 789 amino acid

(B) type: amino acid

(D) topological structure: line style

(ⅱ) molecule type: protein

(ⅹ ⅰ) sequence description: SEQ ID NO:32:Met Asn Lys Asn Asn Thr Lys Leu Ser Thr Arg Ala Leu Pro Ser Phe 15 10 15Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys Asp

20??????????????????25??????????????????30Ile?Met?Asn?Met?Ile?Phe?Lys?Thr?Asp?Thr?Gly?Gly?Asp?Leu?Thr?Leu

35??????????????????40??????????????????45Asp?Glu?Ile?Leu?Lys?Asn?Gln?Gln?Leu?Leu?Asn?Asp?Ile?Ser?Gly?Lys

50??????????????????55??????????????????60Leu?Asp?Gly?Val?Asn?Gly?Ser?Leu?Asn?Asp?Leu?Ile?Ala?Gln?Gly?Asn?65??????????????????70??????????????????75??????????????????80Leu?Asn?Thr?Glu?Leu?Ser?Lys?Glu?Ile?Leu?Lys?Ile?Ala?Asn?Glu?Gln

85??????????????????90??????????????????95Asn?Gln?Val?Leu?Asn?Asp?Val?Asn?Asn?Lys?Leu?Asp?Ala?Ile?Asn?Thr

100?????????????????105?????????????????110Met?Leu?Arg?Val?Tyr?Leu?Pro?Lys?Ile?Thr?Ser?Met?Leu?Ser?Asp?Val

115?????????????????120?????????????????125Met?Lys?Gln?Asn?Tyr?Ala?Leu?Ser?Leu?Gln?Ile?Glu?Tyr?Leu?Ser?Lys

130?????????????????135?????????????????140Gln?Leu?Gln?Glu?Ile?Ser?Asp?Lys?Leu?Asp?Ile?Ile?Asn?Val?Asn?Val145?????????????????150?????????????????155?????????????????160Leu?Ile?Asn?Ser?Thr?Leu?Thr?Glu?Ile?Thr?Pro?Ala?Tyr?Gln?Arg?Ile

165?????????????????170?????????????????175Lys?Tyr?Val?Asn?Glu?Lys?Phe?Glu?Glu?Leu?Thr?Phe?Ala?Thr?Glu?Thr

180?????????????????185?????????????????190Ser?Ser?Lys?Val?Lys?Lys?Asp?Gly?Ser?Pro?Ala?Asp?Ile?Arg?Asp?Glu

195??????????????????200????????????????205Leu?Thr?Glu?Leu?Thr?Glu?Leu?Ala?Lys?Ser?Val?Thr?Lys?Asn?Asp?Val

210?????????????????215?????????????????220Asp?Gly?Phe?Glu?Phe?Tyr?Leu?Asn?Thr?Phe?His?Asp?Val?Met?Val?Gly225?????????????????230?????????????????235?????????????????240Asn?Asn?Leu?Phe?Gly?Arg?Ser?Ala?Leu?Lys?Thr?Ala?Ser?Glu?Leu?Ile

245?????????????????250?????????????????255Thr?Lys?Glu?Asn?Val?Lys?Thr?Ser?Gly?Ser?Glu?Val?Gly?Asn?Val?Tyr

260?????????????????265?????????????????270Asn?Phe?Leu?Ile?Val?Leu?Thr?Ala?Leu?Gln?Ala?Lys?Ala?Phe?Leu?Thr

275????????????????280?????????????????285Leu?Thr?Pro?Cys?Arg?Lys?Leu?Leu?Gly?Leu?Ala?Asp?Ile?Asp?Tyr?Thr

290?????????????????295?????????????????300Ser?Ile?Met?Asn?Glu?His?Leu?Asn?Lys?Glu?Lys?Glu?Glu?Phe?Arg?Val305?????????????????310?????????????????315?????????????????320Asn?Ile?Leu?Pro?Thr?Leu?Ser?Asn?Thr?Phe?Ser?Asn?Pro?Asn?Tyr?Ala

325?????????????????330?????????????????335Lys?Val?Lys?Gly?Ser?Asp?Glu?Asp?Ala?Lys?Met?Ile?Val?Glu?Ala?Lys

340?????????????????345?????????????????350Pro?Gly?His?Ala?Leu?Ile?Gly?Phe?Glu?Ile?Ser?Asn?Asp?Ser?Ile?Thr

355?????????????????360?????????????????365Val?Leu?Lys?Val?Tyr?Glu?Ala?Lys?Leu?Lys?Gln?Asn?Tyr?Gln?Val?Asp

370?????????????????375?????????????????380Lys?Asp?Ser?Leu?Ser?Glu?Val?Ile?Tyr?Gly?Asp?Met?Asp?Lys?Leu?Leu385?????????????????390?????????????????395?????????????????400Cys?Pro?Asp?Gln?Ser?Gly?Gln?Ile?Tyr?Tyr?Thr?Asn?Asn?Ile?Val?Phe

405?????????????????410?????????????????415Pro?Asn?Glu?Tyr?Val?Ile?Thr?Lys?Ile?Asp?Phe?Thr?Lys?Lys?Met?Lys

420?????????????????425?????????????????430Thr?Leu?Arg?Tyr?Glu?Val?Thr?Ala?Asn?Phe?Tyr?Asp?Ser?Ser?Thr?Gly

435?????????????????440?????????????????445Glu?Ile?Asp?Leu?Asn?Lys?Lys?Lys?Val?Glu?Ser?Ser?Glu?Ala?Glu?Tyr

450?????????????????455?????????????????460Arg?Thr?Leu?Ser?Ala?Asn?Asp?Asp?Gly?Val?Tyr?Met?Pro?Leu?Gly?Val465?????????????????470?????????????????475?????????????????480Ile?Ser?Glu?Thr?Phe?Leu?Thr?Pro?Ile?Asn?Gly?Phe?Gly?Leu?Gln?Ala

485?????????????????490?????????????????495Asp?Glu?Asn?Ser?Arg?Leu?Ile?Thr?Leu?Thr?Cys?Lys?Ser?Tyr?Leu?Arg

500?????????????????505?????????????????510Glu?Leu?Leu?Leu?Ala?Thr?Asp?Leu?Ser?Asn?Lys?Glu?Thr?Lys?Leu?Ile

515?????????????????520?????????????????525Val?Pro?Pro?Ser?Gly?Phe?Ile?Ser?Asn?Ile?Val?Glu?Asn?Gly?Ser?Ile

530?????????????????535?????????????????540Glu?Glu?Asp?Asn?Leu?Glu?Pro?Trp?Lys?Ala?Asn?Asn?Lys?Asn?Ala?Tyr545?????????????????550?????????????????555?????????????????560Val?Asp?His?Thr?Gly?Gly?Val?Asn?Gly?Thr?Lys?Ala?Leu?Tyr?Val?His

565?????????????????570?????????????????575Lys?Asp?Gly?Gly?Ile?Ser?Gln?Phe?Ile?Gly?Asp?Lys?Leu?Lys?Pro?Lys

580?????????????????585?????????????????590Thr?Glu?Tyr?Val?Ile?Gln?Tyr?Thr?Val?Lys?Gly?Lys?Pro?Ser?Ile?His

595?????????????????600?????????????????605Leu?Lys?Asp?Glu?Asn?Thr?Gly?Tyr?Ile?His?Tyr?Glu?Asp?Thr?Asn?Asn

610?????????????????615?????????????????620Asn?Leu?Glu?Asp?Tyr?Gln?Thr?Ile?Asn?Lys?Arg?Phe?Thr?Thr?Gly?Thr625?????????????????630?????????????????635?????????????????640Asp?Leu?Lys?Gly?Val?Tyr?Leu?Ile?Leu?Lys?Ser?Gln?Asn?Gly?Asp?Glu

645?????????????????650?????????????????655???Ala?Trp?Gly?Asp?Asn?Phe?Ile?Ile?Leu?Glu?Ile?Ser?Pro?Ser?Glu?Lys

660?????????????????665?????????????????670Leu?Leu?Ser?Pro?Glu?Leu?Ile?Asn?Thr?Asn?Asn?Trp?Thr?Ser?Thr?Gly

675?????????????????680??????????????????685Ser?Thr?Asn?Ile?Ser?Gly?Asn?Thr?Leu?Thr?Leu?Tyr?Gln?Gly?Gly?Arg

690?????????????????695?????????????????700Gly?Ile?Leu?Lys?Gln?Asn?Leu?Gln?Leu?Asp?Ser?Phe?Ser?Thr?Tyr?Arg705?????????????????710?????????????????715?????????????????720Val?Tyr?Phe?Ser?Val?Ser?Gly?Asp?Ala?Asn?Val?Arg?Ile?Arg?Asn?Ser

725?????????????????730?????????????????735Arg?Glu?Val?Leu?Phe?Glu?Lys?Arg?Tyr?Met?Ser?Gly?Ala?Lys?Asp?Val

740?????????????????745?????????????????750Ser?Glu?Met?Phe?Thr?Thr?Lys?Phe?Glu?Lys?Asp?Asn?Phe?Tyr?Ile?Glu

755?????????????????760?????????????????765Leu?Ser?Gln?Gly?Asn?Asn?Leu?Tyr?Gly?Gly?Pro?Ile?Val?His?Phe?Tyr

770?????????????????775?????????????????780Asp?Val?Ser?Ile?Lys785

(2) about the information of SEQ ID NO:33

(ⅰ) sequence signature

(A) length: 30 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: other nucleic acid

(A) describe :/desc=" is used to prepare the forward primer of pCIB5526 "

(ⅲ) the false plan: not

(ⅹ ⅰ) sequence description: SEQ ID NO:33:GGATCCACCA TGAAGACCAA CCAGATCAGC 30

(2) about the information of SEQ ID NO:34

(ⅰ) sequence signature

(A) length: 15 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: other nucleic acid

(A) describe :/desc=" is used to prepare the reverse primer of pCIB5526 "

(ⅲ) the false plan: not

(ⅹ ⅰ) sequence description: SEQ ID NO:34:AAGCTTCAGC TCCTT 15

(2) about the information of SEQ ID NO:35

(ⅰ) sequence signature

(A) length: 2576 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: other nucleic acid

(A) describe :/desc=" synthetic DNA "

(ⅲ) the false plan: not

(ⅸ) feature:

(A) title/key: CDS

(B) position: 9..2564

(D) out of Memory :/remarks=" the corn optimization dna sequence dna of coding VIP1A (a) and the bacillus secretion signal that cuts out is included among the pCIB5526.”

(ⅹ ⅰ) sequence description: SEQ ID NO:35:GATCCACC ATG AAG ACC AAC CAG ATC AGC ACC ACC CAG AAG AAC CAG CAG 50

Met?Lys?Thr?Asn?Gln?Ile?Ser?Thr?Thr?Gln?Lys?Asn?Gln?Gln

825?????????????????830?????????????????835AAG?GAG?ATG?GAC?CGC?AAG?GGC?CTG?CTG?GGC?TAC?TAC?TTC?AAG?GGC?AAG?????????98Lys?Glu?Met?Asp?Arg?Lys?Gly?Leu?Leu?Gly?Tyr?Tyr?Phe?Lys?Gly?Lys

840?????????????????845?????????????????850GAC?TTC?AGC?AAC?CTG?ACC?ATG?TTC?GCC?CCC?ACG?CGT?GAC?AGC?ACC?CTG????????146Asp?Phe?Ser?Asn?Leu?Thr?Met?Phe?Ala?Pro?Thr?Arg?Asp?Ser?Thr?Leu

855?????????????????860?????????????????865ATC?TAC?GAC?CAG?CAG?ACC?GCC?AAC?AAG?CTG?CTG?GAC?AAG?AAG?CAG?CAG????????194Ile?Tyr?Asp?Gln?Gln?Thr?Ala?Asn?Lys?Leu?Leu?Asp?Lys?Lys?GLn?Gln

870?????????????????875?????????????????880GAG?TAC?CAG?AGC?ATC?CGC?TGG?ATC?GGC?CTG?ATC?CAG?AGC?AAG?GAG?ACC????????242Glu?Tyr?Gln?Ser?Ile?Arg?Trp?Ile?Gly?Leu?Ile?Gln?Ser?Lys?Glu?Thr

885?????????????????890?????????????????895GGC?GAC?TTC?ACC?TTC?AAC?CTG?AGC?GAG?GAC?GAG?CAG?GCC?ATC?ATC?GAG????????290Gly?Asp?Phe?Thr?Phe?Asn?Leu?Ser?Glu?Asp?Glu?Gln?Ala?Ile?Ile?Glu900?????????????????905?????????????????910?????????????????915ATC?AAC?GGC?AAG?ATC?ATC?AGC?AAC?AAG?GGC?AAG?GAG?AAG?CAG?GTG?GTG????????338Ile?Asn?Gly?Lys?Ile?Ile?Ser?Asn?Lys?Gly?Lys?Glu?Lys?Gln?Val?Val

920?????????????????925?????????????????930CAC?CTG?GAG?AAG?GGC?AAG?CTG?GTG?CCC?ATC?AAG?ATC?GAG?TAC?CAG?AGC????????386His?Leu?Glu?Lys?Gly?Lys?Leu?Val?Pro?Ile?Lys?Ile?Glu?Tyr?Gln?Ser

935?????????????????940?????????????????945GAC?ACC?AAG?TTC?AAC?ATC?GAC?AGC?AAG?ACC?TTC?AAG?GAG?CTG?AAG?CTT????????434Asp?Thr?Lys?Phe?Asn?Ile?Asp?Ser?Lys?Thr?Phe?Lys?Glu?Leu?Lys?Leu

950?????????????????955?????????????????960TTC?AAG?ATC?GAC?AGC?CAG?AAC?CAG?CCC?CAG?CAG?GTG?CAG?CAG?GAC?GAG????????482Phe?Lys?Ile?Asp?Ser?Gln?Asn?Gln?Pro?Gln?Gln?Val?Gln?Gln?Asp?Glu

965?????????????????970?????????????????975CTG?CGC?AAC?CCC?GAG?TTC?AAC?AAG?AAG?GAG?AGC?CAG?GAG?TTC?CTG?GCC????????530Leu?Arg?Asn?Pro?Glu?Phe?Asn?Lys?Lys?Glu?Ser?Gln?Glu?Phe?Leu?Ala980?????????????????985?????????????????990?????????????????995AAG?CCC?AGC?AAG?ATC?AAC?CTG?TTC?ACC?CAG?CAG?ATG?AAG?CGC?GAG?ATC????????578Lys?Pro?Ser?Lys?Ile?Asn?Leu?Phe?Thr?Gln?Gln?Met?Lys?Arg?Glu?Ile

1000????????????????1005????????????????1010GAC?GAG?GAC?ACC?GAC?ACC?GAC?GGC?GAC?AGC?ATC?CCC?GAC?CTG?TGG?GAG????????626Asp?Glu?Asp?Thr?Asp?Thr?Asp?Gly?Asp?Ser?Ile?Pro?Asp?Leu?Trp?Glu

1015????????????????1020????????????????1025GAG?AAC?GGC?TAC?ACC?ATC?CAG?AAC?CGC?ATC?GCC?GTG?AAG?TGG?GAC?GAC????????674Glu?Asn?Gly?Tyr?Thr?Ile?Gln?Asn?Arg?Ile?Ala?Val?Lys?Trp?Asp?Asp

1030????????????????1035????????????????1040AGC?CTG?GCT?AGC?AAG?GGC?TAC?ACC?AAG?TTC?GTG?AGC?AAC?CCC?CTG?GAG????????722Ser?Leu?Ala?Ser?Lys?Gly?Tyr?Thr?Lys?Phe?Val?Ser?Asn?Pro?Leu?Glu

1045????????????????1050????????????????1055AGC?CAC?ACC?GTG?GGC?GAC?CCC?TAC?ACC?GAC?TAC?GAG?AAG?GCC?GCC?CGC????????770Ser?His?Thr?Val?Gly?Asp?Pro?Tyr?Thr?Asp?Tyr?Glu?Lys?Ala?Ala?Arg1060????????????????1065???????????????1070?????????????????1075GAC?CTG?GAC?CTG?AGC?AAC?GCC?AAG?GAG?ACC?TTC?AAC?CCC?CTG?GTG?GCC????????818Asp?Leu?Asp?Leu?Ser?Asn?Ala?Lys?Glu?Thr?Phe?Asn?Pro?Leu?Val?Ala

1080????????????????1085????????????????1090GCC?TTC?CCC?AGC?GTG?AAC?GTG?AGC?ATG?GAG?AAG?GTG?ATC?CTG?AGC?CCC????????866Ala?Phe?Pro?Ser?Val?Asn?Val?Ser?Met?Glu?Lys?Val?Ile?Leu?Ser?Pro

1095????????????????1100????????????????1105AAC?GAG?AAC?CTG?AGC?AAC?AGC?GTG?GAG?AGC?CAC?TCG?AGC?ACC?AAC?TGG????????914Asn?Glu?Asn?Leu?Ser?Asn?Ser?Val?Glu?Ser?His?Ser?Ser?Thr?Asn?Trp

1110????????????????1115????????????????1120AGC?TAC?ACC?AAC?ACC?GAG?GGC?GCC?AGC?GTG?GAG?GCC?GGC?ATC?GGT?CCC????????962Ser?Tyr?Thr?Asn?Thr?Glu?Gly?Ala?Ser?Val?Glu?Ala?Gly?Ile?Gly?Pro

1125????????????????1130????????????????1135AAG?GGC?ATC?AGC?TTC?GGC?GTG?AGC?GTG?AAC?TAC?CAG?CAC?AGC?GAG?ACC???????1010Lys?Gly?Ile?Ser?Phe?Gly?Val?Ser?Val?Asn?Tyr?Gln?His?Ser?Glu?Thr1140????????????????1145????????????????1150????????????????1155GTG?GCC?CAG?GAG?TGG?GGC?ACC?AGC?ACC?GGC?AAC?ACC?AGC?CAG?TTC?AAC???????1058Val?Ala?Gln?Glu?Trp?Gly?Thr?Ser?Thr?Gly?Asn?Thr?Ser?Gln?Phe?Asn

1160????????????????1165????????????????1170ACC?GCC?AGC?GCC?GGC?TAC?CTG?AAC?GCC?AAC?GTG?CGC?TAC?AAC?AAC?GTG???????1106Thr?Ala?Ser?Ala?Gly?Tyr?Leu?Asn?Ala?Asn?Val?Arg?Tyr?Asn?Asn?Val

1175????????????????1180????????????????1185GGC?ACC?GGC?GCC?ATC?TAC?GAC?GTG?AAG?CCC?ACC?ACC?AGC?TTC?GTG?CTG???????1154Gly?Thr?Gly?Ala?Ile?Tyr?Asp?Val?Lys?Pro?Thr?Thr?Ser?Phe?Val?Leu

1190????????????????1195????????????????1200AAC?AAC?GAC?ACC?ATC?GCC?ACC?ATC?ACC?GCC?AAG?TCG?AAT?TCC?ACC?GCC???????1202Asn?Asn?Asp?Thr?Ile?Ala?Thr?Ile?Thr?Ala?Lys?Ser?Asn?Ser?Thr?Ala

1205????????????????1210????????????????1215CTG?AAC?ATC?AGC?CCC?GGC?GAG?AGC?TAC?CCC?AAG?AAG?GGC?CAG?AAC?GGC???????1250Leu?Asn?Ile?Ser?Pro?Gly?Glu?Ser?Tyr?Pro?Lys?Lys?Gly?Gln?Asn?Gly1220????????????????1225????????????????1230????????????????1235ATC?GCC?ATC?ACC?AGC?ATG?GAC?GAC?TTC?AAC?AGC?CAC?CCC?ATC?ACC?CTG???????1298Ile?Ala?Ile?Thr?Ser?Met?Asp?Asp?Phe?Asn?Ser?His?Pro?Ile?Thr?Leu

1240????????????????1245????????????????1250AAC?AAG?AAG?CAG?GTG?GAC?AAC?CTG?CTG?AAC?AAC?AAG?CCC?ATG?ATG?CTG???????1346Asn?Lys?Lys?Gln?Val?Asp?Asn?Leu?Leu?Asn?Asn?Lys?Pro?Met?Met?Leu

1255????????????????1260????????????????1265GAG?ACC?AAC?CAG?ACC?GAC?GGC?GTC?TAC?AAG?ATC?AAG?GAC?ACC?CAC?GGC???????1394Glu?Thr?Asn?Gln?Thr?Asp?Gly?Val?Tyr?Lys?Ile?Lys?Asp?Thr?His?Gly

1270????????????????1275????????????????1280AAC?ATC?GTG?ACG?GGC?GGC?GAG?TGG?AAC?GGC?GTG?ATC?CAG?CAG?ATC?AAG???????1442Asn?Ile?Val?Thr?Gly?Gly?Glu?Trp?Asn?Gly?Val?Ile?Gln?Gln?Ile?Lys

1285????????????????1290????????????????1295GCC?AAG?ACC?GCC?AGC?ATC?ATC?GTC?GAC?GAC?GGC?GAG?CGC?GTG?GCC?GAG???????1490Ala?Lys?Thr?Ala?Ser?Ile?Ile?Val?Asp?Asp?Gly?Glu?Arg?Val?Ala?Glu1300????????????????1305????????????????1310????????????????1315AAG?CGC?GTG?GCC?GCC?AAG?GAC?TAC?GAG?AAC?CCC?GAG?GAC?AAG?ACC?CCC???????1538Lys?Arg?Val?Ala?Ala?Lys?Asp?Tyr?Glu?Asn?Pro?Glu?Asp?Lys?Thr?Pro

1320????????????????1325????????????????1330AGC?CTG?ACC?CTG?AAG?GAC?GCC?CTG?AAG?CTG?AGC?TAC?CCC?GAC?GAG?ATC???????1586Ser?Leu?Thr?Leu?Lys?Asp?Ala?Leu?Lys?Leu?Ser?Tyr?Pro?Asp?Glu?Ile

1335????????????????1340????????????????1345AAG?GAG?ATC?GAG?GGC?TTG?CTG?TAC?TAC?AAG?AAC?AAG?CCC?ATC?TAC?GAG???????1634Lys?Glu?Ile?Glu?Gly?Leu?Leu?Tyr?Tyr?Lys?Asn?Lys?Pro?Ile?Tyr?Glu

1350????????????????1355????????????????1360AGC?AGC?GTG?ATG?ACC?TAT?CTA?GAC?GAG?AAC?ACC?GCC?AAG?GAG?GTG?ACC???????1682Ser?Ser?Val?Met?Thr?Tyr?Leu?Asp?Glu?Asn?Thr?Ala?Lys?Glu?Val?Thr

1365????????????????1370???????????????1375AAG?CAG?CTG?AAC?GAC?ACC?ACC?GGC?AAG?TTC?AAG?GAC?GTG?AGC?CAC?CTG????????1730Lys?Gln?Leu?Asn?Asp?Thr?Thr?Gly?Lys?Phe?Lys?Asp?Val?Ser?His?Leu1380????????????????1385????????????????1390????????????????1395TAC?GAC?GTG?AAG?CTG?ACC?CCC?AAG?ATG?AAC?GTG?ACC?ATC?AAG?CTG?AGC????????1778Tyr?Asp?Val?Lys?Leu?Thr?Pro?Lys?Met?Asn?Val?Thr?Ile?Lys?Leu?Ser

1400????????????????1405????????????????1410ATC?CTG?TAC?GAC?AAC?GCC?GAG?AGC?AAC?GAC?AAC?AGC?ATC?GGC?AAG?TGG????????1826Ile?Leu?Tyr?Asp?Asn?Ala?Glu?Ser?Asn?Asp?Asn?Ser?Ile?Gly?Lys?Trp

1415????????????????1420????????????????1425ACC?AAC?ACC?AAC?ATC?GTG?AGC?GGC?GGC?AAC?AAC?GGC?AAG?AAG?CAG?TAC????????1874Thr?Asn?Thr?Asn?Ile?Val?Ser?Gly?Gly?Asn?Asn?Gly?Lys?Lys?Gln?Tyr

1430????????????????1435????????????????1440AGC?AGC?AAC?AAC?CCC?GAC?GCC?AAC?CTG?ACC?CTG?AAC?ACC?GAC?GCC?CAG????????1922Ser?Ser?Asn?Asn?Pro?Asp?Ala?Asn?Leu?Thr?Leu?Asn?Thr?Asp?Ala?Gln

1445????????????????1450????????????????1455GAG?AAG?CTG?AAC?AAG?AAC?CGC?GAC?TAC?TAC?ATC?AGC?CTG?TAC?ATG?AAG????????1970Glu?Lys?Leu?Asn?Lys?Asn?Arg?Asp?Tyr?Tyr?Ile?Ser?Leu?Tyr?Met?Lys1460????????????????1465????????????????1470????????????????1475AGC?GAG?AAG?AAC?ACC?CAG?TGC?GAG?ATC?ACC?ATC?GAC?GGC?GAG?ATA?TAC????????2018Ser?Glu?Lys?Asn?Thr?Gln?Cys?Glu?Ile?Thr?Ile?Asp?Gly?Glu?Ile?Tyr

1480????????????????1485????????????????1490CCC?ATC?ACC?ACC?AAG?ACC?GTG?AAC?GTG?AAC?AAG?GAC?AAC?TAC?AAG?CGC????????2066Pro?Ile?Thr?Thr?Lys?Thr?Val?Asn?Val?Asn?Lys?Asp?Asn?Tyr?Lys?Arg

1495????????????????1500????????????????1505CTG?GAC?ATC?ATC?GCC?CAC?AAC?ATC?AAG?AGC?AAC?CCC?ATC?AGC?AGC?CTG????????2114Leu?Asp?Ile?Ile?Ala?His?Asn?Ile?Lys?Ser?Asn?Pro?Ile?Ser?Ser?Leu

1510????????????????1515????????????????1520CAC?ATC?AAG?ACC?AAC?GAC?GAG?ATC?ACC?CTG?TTC?TGG?GAC?GAC?ATA?TCG????????2162His?Ile?Lys?Thr?Asn?Asp?Glu?Ile?Thr?Leu?Phe?Trp?Asp?Asp?Ile?Ser

1525????????????????1530????????????????1535ATT?ACC?GAC?GTC?GCC?AGC?ATC?AAG?CCC?GAG?AAC?CTG?ACC?GAC?AGC?GAG????????2210Ile?Thr?Asp?Val?Ala?Ser?Ile?Lys?Pro?Glu?Asn?Leu?Thr?Asp?Ser?Glu1540????????????????1545????????????????1550????????????????1555ATC?AAG?CAG?ATA?TAC?AGT?CGC?TAC?GGC?ATC?AAG?CTG?GAG?GAC?GGC?ATC????????2258Ile?Lys?Gln?Ile?Tyr?Ser?Arg?Tyr?Gly?Ile?Lys?Leu?Glu?Asp?Gly?Ile

1560????????????????1565????????????????1570CTG?ATC?GAC?AAG?AAA?GGC?GGC?ATC?CAC?TAC?GGC?GAG?TTC?ATC?AAC?GAG????????2306Leu?Ile?Asp?Lys?Lys?Gly?Gly?Ile?His?Tyr?Gly?Glu?Phe?Ile?Asn?Glu

1575????????????????1580????????????????1585GCC?AGC?TTC?AAC?ATC?GAG?CCC?CTG?CAG?AAC?TAC?GTG?ACC?AAG?TAC?GAG????????2354Ala?Ser?Phe?Asn?Ile?Glu?Pro?Leu?Gln?Asn?Tyr?Val?Thr?Lys?Tyr?Glu

1590????????????????????1595????????????????1600GTG?ACC?TAC?AGC?AGC?GAG?CTG?GGC?CCC?AAC?GTG?AGC?GAC?ACC?CTG?GAG???????2402Val?Thr?Tyr?Ser?Ser?Glu?Leu?Gly?Pro?Asn?Val?Ser?Asp?Thr?Leu?Glu

1605????????????????1610????????????????1615AGC?GAC?AAG?ATT?TAC?AAG?GAC?GGC?ACC?ATC?AAG?TTC?GAC?TTC?ACC?AAG???????2450Ser?Asp?Lys?Ile?Tyr?Lys?Asp?Gly?Thr?Ile?Lys?Phe?Asp?Phe?Thr?Lys1620????????????????1625????????????????1630????????????????1635TAC?AGC?AAG?AAC?GAG?CAG?GGC?CTG?TTC?TAC?GAC?AGC?GGC?CTG?AAC?TGG???????2498Tyr?Ser?Lys?Asn?Glu?Gln?Gly?Leu?Phe?Tyr?Asp?Ser?Gly?Leu?Asn?Trp

1640????????????????1645????????????????1650GAC?TTC?AAG?ATC?AAC?GCC?ATC?ACC?TAC?GAC?GGC?AAG?GAG?ATG?AAC?GTG???????2546Asp?Phe?Lys?Ile?Asn?Ala?Ile?Thr?Tyr?Asp?Gly?Lys?Glu?Met?Asn?Val

1655????????????????1660????????????????1665TTC?CAC?CGC?TAC?AAC?AAG?TAGATCTGAG?CT?????????????????????????????????2576Phe?His?Arg?Tyr?Asn?Lys

1670

(2) about the information of SEQ ID NO:36

(ⅰ) sequence signature

(A) length: 852 amino acid

(B) type: amino acid

(D) topological structure: line style

(ⅱ) molecule type: protein

(ⅹ ⅰ) sequence description: SEQ ID NO:36:Met Lys Thr Asn Gln Ile Ser Thr Thr Gln Lys Asn Gln Gln Lys Glu 15 10 15Met Asp Arg Lys Gly Leu Leu Gly Tyr Tyr Phe Lys Gly Lys Asp Phe

20??????????????????25??????????????????30Ser?Asn?Leu?Thr?Met?Phe?Ala?Pro?Thr?Arg?Asp?Ser?Thr?Leu?Ile?Tyr

35??????????????????40??????????????????45Asp?Gln?Gln?Thr?Ala?Asn?Lys?Leu?Leu?Asp?Lys?Lys?Gln?Gln?Glu?Tyr

50??????????????????55??????????????????60Gln?Ser?Ile?Arg?Trp?Ile?Gly?Leu?Ile?Gln?Ser?Lys?Glu?Thr?Gly?Asp?65??????????????????70??????????????????75??????????????????80Phe?Thr?Phe?Asn?Leu?Ser?Glu?Asp?Glu?Gln?Ala?Ile?Ile?Glu?Ile?Asn

85??????????????????90??????????????????95Gly?Lys?Ile?Ile?Ser?Asn?Lys?Gly?Lys?Glu?Lys?Gln?Val?Val?His?Leu

100?????????????????105?????????????????110Glu?Lys?Gly?Lys?Leu?Val?Pro?Ile?Lys?Ile?Glu?Tyr?Gln?Ser?Asp?Thr

115?????????????????120?????????????????125Lys?Phe?Asn?Ile?Asp?Ser?Lys?Thr?Phe?Lys?Glu?Leu?Lys?Leu?Phe?Lys

130?????????????????135?????????????????140Ile?Asp?Ser?Gln?Asn?Gln?Pro?Gln?Gln?Val?Gln?Gln?Asp?Glu?Leu?Arg145?????????????????150?????????????????155?????????????????160Asn?Pro?Glu?Phe?Asn?Lys?Lys?Glu?Ser?Gln?Glu?Phe?Leu?Ala?Lys?Pro

165?????????????????170?????????????????175Ser?Lys?Ile?Asn?Leu?Phe?Thr?Gln?Gln?Met?Lys?Arg?Glu?Ile?Asp?Glu

180?????????????????185?????????????????190Asp?Thr?Asp?Thr?Asp?Gly?Asp?Ser?Ile?Pro?Asp?Leu?Trp?Glu?Glu?Asn

195??????????????????200?????????????????205Gly?Tyr?Thr?Ile?Gln?Asn?Arg?Ile?Ala?Val?Lys?Trp?Asp?Asp?Ser?Leu

210?????????????????215?????????????????220Ala?Ser?Lys?Gly?Tyr?Thr?Lys?Phe?Val?Ser?Asn?Pro?Leu?Glu?Ser?His225?????????????????230?????????????????235?????????????????240Thr?Val?Gly?Asp?Pro?Tyr?Thr?Asp?Tyr?Glu?Lys?Ala?Ala?Arg?Asp?Leu

245??????????????????250????????????????255Asp?Leu?Ser?Asn?Ala?Lys?Glu?Thr?Phe?Asn?Pro?Leu?Val?Ala?Ala?Phe

260??????????????????265????????????????270Pro?Ser?Val?Asn?Val?Ser?Met?Glu?Lys?Val?Ile?Leu?Ser?Pro?Asn?Glu

275??????????????????280????????????????285Asn?Leu?Ser?Asn?Ser?Val?Glu?Ser?His?Ser?Ser?Thr?Asn?Trp?Ser?Tyr

290?????????????????295?????????????????300

Thr?Asn?Thr?Glu?Gly?Ala?Ser?Val?Glu?Ala?Gly?Ile?Gly?Pro?Lys?Gly305?????????????????310?????????????????315?????????????????320Ile?Ser?Phe?Gly?Val?Ser?Val?Asn?Tyr?Gln?His?Ser?Glu?Thr?Val?Ala

325?????????????????330?????????????????335Gln?Glu?Trp?Gly?Thr?Ser?Thr?Gly?Asn?Thr?Ser?Gln?Phe?Asn?Thr?Ala

340??????????????????345????????????????350Ser?Ala?Gly?Tyr?Leu?Asn?Ala?Asn?Val?Arg?Tyr?Asn?Asn?Val?Gly?Thr

355?????????????????360?????????????????365Gly?Ala?Ile?Tyr?Asp?Val?Lys?Pro?Thr?Thr?Ser?Phe?Val?Leu?Asn?Asn

370?????????????????375?????????????????380Asp?Thr?Ile?Ala?Thr?Ile?Thr?Ala?Lys?Ser?Asn?Ser?Thr?Ala?Leu?Asn385?????????????????390?????????????????395?????????????????400Ile?Ser?Pro?Gly?Glu?Ser?Tyr?Pro?Lys?Lys?Gly?Gln?Asn?Gly?Ile?Ala

405?????????????????410?????????????????415Ile?Thr?Ser?Met?Asp?Asp?Phe?Asn?Ser?His?Pro?Ile?Thr?Leu?Asn?Lys

420?????????????????425?????????????????430Lys?Gln?Val?Asp?Asn?Leu?Leu?Asn?Asn?Lys?Pro?Met?Met?Leu?Glu?Thr

435?????????????????440?????????????????445Asn?Gln?Thr?Asp?Gly?Val?Tyr?Lys?Ile?Lys?Asp?Thr?His?Gly?Asn?Ile

450?????????????????455?????????????????460Val?Thr?Gly?Gly?Glu?Trp?Asn?Gly?Val?Ile?Gln?Gln?Ile?Lys?Ala?Lys465?????????????????470?????????????????475?????????????????480Thr?Ala?Ser?Ile?Ile?Val?Asp?Asp?Gly?Glu?Arg?Val?Ala?Glu?Lys?Arg

485?????????????????490?????????????????495Val?Ala?Ala?Lys?Asp?Tyr?Glu?Asn?Pro?Glu?Asp?Lys?Thr?Pro?Ser?Leu

500?????????????????505?????????????????510Thr?Leu?Lys?Asp?Ala?Leu?Lys?Leu?Ser?Tyr?Pro?Asp?Glu?Ile?Lys?Glu

515?????????????????520?????????????????525Ile?Glu?Gly?Leu?Leu?Tyr?Tyr?Lys?Asn?Lys?Pro?Ile?Tyr?Glu?Ser?Ser

530??????????????????535????????????????540Val?Met?Thr?Tyr?Leu?Asp?Glu?Asn?Thr?Ala?Lys?Glu?Val?Thr?Lys?Gln545?????????????????550?????????????????555?????????????????560Leu?Asn?Asp?Thr?Thr?Gly?Lys?Phe?Lys?Asp?Val?Ser?His?Leu?Tyr?Asp

565?????????????????570?????????????????575Val?Lys?Leu?Thr?Pro?Lys?Met?Asn?Val?Thr?Ile?Lys?Leu?Ser?Ile?Leu

580?????????????????585?????????????????590Tyr?Asp?Asn?Ala?Glu?Ser?Asn?Asp?Asn?Ser?Ile?Gly?Lys?Trp?Thr?Asn

595?????????????????600?????????????????605Thr?Asn?Ile?Val?Ser?Gly?Gly?Asn?Asn?Gly?Lys?Lys?Gln?Tyr?Ser?Ser

610?????????????????615?????????????????620Asn?Asn?Pro?Asp?Ala?Asn?Leu?Thr?Leu?Asn?Thr?Asp?Ala?Gln?Glu?Lys625?????????????????630?????????????????635?????????????????640Leu?Asn?Lys?Asn?Arg?Asp?Tyr?Tyr?Ile?Ser?Leu?Tyr?Met?Lys?Ser?Glu

645?????????????????650?????????????????655Lys?Asn?Thr?Gln?Cys?Glu?Ile?Thr?Ile?Asp?Gly?Glu?Ile?Tyr?Pro?Ile

660?????????????????665?????????????????670Thr?Thr?Lys?Thr?Val?Asn?Val?Asn?Lys?Asp?Asn?Tyr?Lys?Arg?Leu?Asp

675?????????????????680?????????????????685Ile?Ile?Ala?His?Asn?Ile?Lys?Ser?Asn?Pro?Ile?Ser?Ser?Leu?His?Ile

690?????????????????695?????????????????700Lys?Thr?Asn?Asp?Glu?Ile?Thr?Leu?Phe?Trp?Asp?Asp?Ile?Ser?Ile?Thr705??????????????????710????????????????715?????????????????720Asp?Val?Ala?Ser?Ile?Lys?Pro?Glu?Asn?Leu?Thr?Asp?Ser?Glu?Ile?Lys

725?????????????????730?????????????????735Gln?Ile?Tyr?Ser?Arg?Tyr?Gly?Ile?Lys?Leu?Glu?Asp?Gly?Ile?Leu?Ile

740?????????????????745?????????????????750Asp?Lys?Lys?Gly?Gly?Ile?His?Tyr?Gly?Glu?Phe?Ile?Asn?Glu?Ala?Ser

755?????????????????760?????????????????765Phe?Asn?Ile?Glu?Pro?Leu?Gln?Asn?Tyr?Val?Thr?Lys?Tyr?Glu?Val?Thr

770?????????????????775?????????????????780Tyr?Ser?Ser?Glu?Leu?Gly?Pro?Asn?Val?Ser?Asp?Thr?Leu?Glu?Ser?Asp785?????????????????790?????????????????795?????????????????800Lys?Ile?Tyr?Lys?Asp?Gly?Thr?Ile?Lys?Phe?Asp?Phe?Thr?Lys?Tyr?Ser

805?????????????????810?????????????????815Lys?Asn?Glu?Gln?Gly?Leu?Phe?Tyr?Asp?Ser?Gly?Leu?Asn?Trp?Asp?Phe

820?????????????????825?????????????????830Lys?Ile?Asn?Ala?Ile?Thr?Tyr?Asp?Gly?Lys?Glu?Met?Asn?Val?Phe?His

835?????????????????840?????????????????845Arg?Tyr?Asn?Lys???850

(2) about the information of SEQ ID NO:37

(ⅰ) sequence signature

(A) length: 32 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: other nucleic acid

(A) describe :/desc=" is used to prepare the forward primer of pCIB5527 "

(ⅲ) the false plan: not

(ⅹ ⅰ) sequence description: SEQ ID NO:37:GGATCCACCA TGCIGCAGAA CCTGAGATCAC 32

(2) about the information of SEQ ID NO:38

(ⅰ) sequence signature

(A) 18 base-pairs of length

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: other nucleic acid

(A) describe :/desc=" is used to prepare the reverse primer of pCIB5527 "

(ⅲ) the false plan: not

(ⅹ ⅰ) sequence description: SEQ ID NO:38:AAGCTTCCAC TCCTTCTC 18

(2) about the information of SEQ ID NO:39

(ⅰ) sequence signature

(A) length: 1241 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: other nucleic acid

(A) describe :/desc=" synthetic DNA "

(ⅲ) the false plan: not

(ⅸ) feature:

(A) title/key: CDS

(B) position: 9.1238

(D) out of Memory :/remarks=" the corn optimization dna sequence dna of coding VIP2A (a) and the bacillus secretion signal that cuts out is included among the pCIB5527.”

(ⅹ ⅰ) sequence description: SEQ ID NO:39:GATCCACC ATG CTG CAG AAC CTG AAG ATC ACC GAC AAG GTG GAG GAC TTC 50

Met?Leu?Gln?Asn?Leu?Lys?Ile?Thr?Asp?Lys?Val?Glu?Asp?Phe

855?????????????????860?????????????????865AAG?GAG?GAC?AAG?GAG?AAG?GCC?AAG?GAG?TGG?GGC?AAG?GAG?AAG?GAG?AAG??????????98Lys?Glu?Asp?Lys?Glu?Lys?Ala?Lys?Glu?Trp?Gly?Lys?Glu?Lys?Glu?Lys

870?????????????????875?????????????????880GAG?TGG?AAG?CTT?ACC?GCC?ACC?GAG?AAG?GGC?AAG?ATG?AAC?AAC?TTC?CTG?????????146Glu?Trp?Lys?Leu?Thr?Ala?Thr?Glu?Lys?Gly?Lys?Met?Asn?Asn?Phe?Leu

885?????????????????890?????????????????895GAC?AAC?AAG?AAC?GAC?ATC?AAG?ACC?AAC?TAC?AAG?GAG?ATC?ACC?TTC?AGC????????194Asp?Asn?Lys?Asn?Asp?Ile?Lys?Thr?Asn?Tyr?Lys?Glu?Ile?Thr?Phe?Ser

900?????????????????905?????????????????910ATA?GCC?GGC?AGC?TTC?GAG?GAC?GAG?ATC?AAG?GAC?CTG?AAG?GAG?ATC?GAC????????242Ile?Ala?Gly?Ser?Phe?Glu?Asp?Glu?Ile?Lys?Asp?Leu?Lys?Glu?Ile?Asp915?????????????????920?????????????????925?????????????????930AAG?ATG?TTC?GAC?AAG?ACC?AAC?CIG?AGC?AAC?AGC?ATC?ATC?ACC?TAC?AAG????????290Lys?Met?Phe?Asp?Lys?Thr?Asn?Leu?Ser?Asn?Ser?Ile?Ile?Thr?Tyr?Lys

935????????????????????940??????????????????945AAC?GTG?GAG?CCC?ACC?ACC?ATC?GGC?TTC?AAC?AAG?AGC?CTG?ACC?GAG?GGC????????338Asn?Val?Glu?Pro?Thr?Thr?Ile?Gly?Phe?Asn?Lys?Ser?Leu?Thr?Glu?Gly

950?????????????????955?????????????????960AAC?ACC?ATC?AAC?AGC?GAC?GCC?ATG?GCC?CAG?TTC?AAG?GAG?CAG?TTC?CTG????????386Asn?Thr?Ile?Asn?Ser?Asp?Ala?Met?Ala?Gln?Phe?Lys?Glu?Gln?Phe?Leu

965?????????????????970?????????????????975GAC?CGC?GAC?ATC?AAG?TTC?GAC?AGC?TAC?CIG?GAC?ACC?CAC?CTG?ACC?GCC????????434Asp?Arg?Asp?Ile?Lys?Phe?Asp?Ser?Tyr?Leu?Asp?Thr?His?Leu?Thr?Ala

980??????????????????985????????????????990CAG?CAG?GTG?AGC?AGC?AAG?GAG?CGC?GTG?ATC?CTG?AAG?GTG?ACC?GTC?CCC????????482Gln?Gln?Val?Ser?Ser?Lys?Glu?Arg?Val?Ile?Leu?Lys?Val?Thr?Val?Pro995?????????????????1000????????????????1005????????????????1010AGC?GGC?AAG?GGC?AGC?ACC?ACC?CCC?ACC?AAG?GCC?GGC?GTG?ATC?CTG?AAC????????530Ser?Gly?Lys?Gly?Ser?Thr?Thr?Pro?Thr?Lys?Ala?Gly?Val?Ile?Leu?Asn

1015????????????????1020????????????????1025AAC?AGC?GAG?TAC?AAG?ATG?CTG?ATC?GAC?AAC?GGC?TAC?ATG?GTG?CAC?GTG????????578Asn?Ser?Glu?Tyr?Lys?Met?Leu?Ile?Asp?Asn?Gly?Tyr?Met?Val?His?Val

1030????????????????1035????????????????1040GAC?AAG?GTG?AGC?AAG?GTG?GTG?AAG?AAG?GGC?GTG?GAG?TGC?CTC?CAG?ATC????????626Asp?Lys?Val?Ser?Lys?Val?Val?Lys?Lys?Gly?Val?Glu?Cys?Leu?Gln?Ile

1045????????????????1050????????????????1055GAG?GGC?ACC?CTG?AAG?AAG?AGT?CTA?GAC?TTC?AAG?AAC?GAC?ATC?AAC?GCC????????674Glu?Gly?Thr?Leu?Lys?Lys?Ser?Leu?Asp?Phe?Lys?Asn?Asp?Ile?Asn?Ala???1060?????????????????1065????????????????1070GAG?GCC?CAC?AGC?TGG?GGC?ATG?AAG?AAC?TAC?GAG?GAG?TGG?GCC?AAG?GAC????????722Glu?Ala?His?Ser?Trp?Gly?Met?Lys?Asn?Tyr?Glu?Glu?Trp?Ala?Lys?Asp1075????????????????1080????????????????1085????????????????1090CTG?ACC?GAC?AGC?CAG?CGC?GAG?GCC?CTG?GAC?GGC?TAC?GCC?CGC?CAG?GAC????????770Leu?Thr?Asp?Ser?Gln?Arg?Glu?Ala?Leu?Asp?Gly?Tyr?Ala?Arg?Gln?Asp

1095????????????????1100????????????????1105TAC?AAG?GAG?ATC?AAC?AAC?TAC?CTG?CGC?AAC?CAG?GGC?GGC?AGC?GGC?AAC????????818Tyr?Lys?Glu?Ile?Asn?Asn?Tyr?Leu?Arg?Asn?Gln?Gly?Gly?Ser?Gly?Asn

1110????????????????1115????????????????1120GAG?AAG?CTG?GAC?GCC?CAG?ATC?AAG?AAC?ATC?AGC?GAC?GCC?CTG?GGC?AAG????????866Glu?Lys?Leu?Asp?Ala?Gln?Ile?Lys?Asn?Ile?Ser?Asp?Ala?Leu?Gly?Lys

1125????????????????1130????????????????1135AAG?CCC?ATC?CCC?GAG?AAC?ATC?ACC?GTG?TAC?CGC?TGG?TGC?GGC?ATG?CCC????????914Lys?Pro?Ile?Pro?Glu?Asn?Ile?Thr?Val?Tyr?Arg?Trp?Cys?Gly?Met?Pro

1140????????????????1145????????????????1150GAG?TTC?GGC?TAC?CAG?ATC?AGC?GAC?CCC?CTG?CCC?AGC?CTG?AAG?GAC?TTC????????962Glu?Phe?Gly?Tyr?Gln?Ile?Ser?Asp?Pro?Leu?Pro?Ser?Leu?Lys?Asp?Phe1155????????????????1160????????????????1165????????????????1170GAG?GAG?CAG?TTC?CTG?AAC?ACC?ATC?AAG?GAG?GAC?AAG?GGC?TAC?ATG?AGC???????1010Glu?Glu?Gln?Phe?Leu?Asn?Thr?Ile?Lys?Glu?Asp?Lys?Gly?Tyr?Met?Ser

1175????????????????1180????????????????1185ACC?AGC?CTG?AGC?AGC?GAG?CGC?CTG?GCC?GCC?TTC?GGC?AGC?CGC?AAG?ATC???????1058Thr?Ser?Leu?Ser?Ser?Glu?Arg?Leu?Ala?Ala?Phe?Gly?Ser?Arg?Lys?Tle

1190????????????????1195????????????????1200ATC?CTG?CGC?CTG?CAG?GTG?CCC?AAG?GGC?AGC?ACT?GGT?GCC?TAC?CTG?AGC???????1106Ile?Leu?Arg?Leu?Gln?Val?Pro?Lys?Gly?Ser?Thr?Gly?Ala?Tyr?Leu?Ser

1205????????????????1210????????????????1215GCC?ATC?GGC?GGC?TTC?GCC?AGC?GAG?AAG?GAG?ATC?CTG?CTG?GAT?AAG?GAC???????1154Ala?Ile?Gly?Gly?Phe?Ala?Ser?Glu?Lys?Glu?Ile?Leu?Leu?Asp?Lys?Asp

1220????????????????1225????????????????1230AGC?AAG?TAC?CAC?ATC?GAC?AAG?GTG?ACC?GAG?GTG?ATC?ATC?AAG?GGC?GTG???????1202Ser?Lys?Tyr?His?Ile?Asp?Lys?Val?Thr?Glu?Val?Ile?Ile?Lys?Gly?Val1235????????????????1240????????????????1245????????????????1250AAG?CGC?TAC?GTG?GTG?GAC?GCC?ACC?CTG?CTG?ACC?AAC?TAG???????????????????1241Lys?Arg?Tyr?Val?Val?Asp?Ala?Thr?Leu?Leu?Thr?Asn

1255????????????????1260

(2) about the information of SEQ ID NO:40

(ⅰ) sequence signature

(A) length: 410 amino acid

(B) type: amino acid

(D) topological structure: line style

(ⅱ) molecule type: protein

(ⅹ ⅰ) sequence description: SEQ ID NO:40:Met Leu Gln Asn Leu Lys Ile Thr Asp Lys Val Glu Asp Phe Lys Glu 15 10 15Asp Lys Glu Lys Ala Lys Glu Trp Gly Lys Glu Lys Glu Lys Glu Trp

20??????????????????25??????????????????30Lys?Leu?Thr?Ala?Thr?Glu?Lys?Gly?Lys?Met?Asn?Asn?Phe?Leu?Asp?Asn

35??????????????????40??????????????????45Lys?Asn?Asp?Ile?Lys?Thr?Asn?Tyr?Lys?Glu?Ile?Thr?Phe?Ser?Ile?Ala

50???????????????????55??????????????????60Gly?Ser?Phe?Glu?Asp?Glu?Ile?Lys?Asp?Leu?Lys?Glu?Ile?Asp?Lys?Met?65??????????????????70??????????????????75??????????????????80Phe?Asp?Lys?Thr?Asn?Leu?Ser?Asn?Ser?Ile?Ile?Thr?Tyr?Lys?Asn?Val

85??????????????????90??????????????????95Glu?Pro?Thr?Thr?Ile?Gly?Phe?Asn?Lys?Ser?Leu?Thr?Glu?Gly?Asn?Thr

100?????????????????105?????????????????110Ile?Asn?Ser?Asp?Ala?Met?Ala?Gln?Phe?Lys?Glu?Gln?Phe?Leu?Asp?Arg

115?????????????????120?????????????????125Asp?Ile?Lys?Phe?Asp?Ser?Tyr?Leu?Asp?Thr?His?Leu?Thr?Ala?Gln?Gln

130?????????????????135?????????????????140Val?Ser?Ser?Lys?Glu?Arg?Val?Ile?Leu?Lys?Val?Thr?Val?Pro?Ser?Gly145?????????????????150?????????????????155?????????????????160Lys?Gly?Ser?Thr?Thr?Pro?Thr?Lys?Ala?Gly?Val?Ile?Leu?Asn?Asn?Ser

165?????????????????170?????????????????175Glu?Tyr?Lys?Met?Leu?Ile?Asp?Asn?Gly?Tyr?Met?Val?His?Val?Asp?Lys

180?????????????????185??????????????????190Val?Ser?Lys?Val?Val?Lys?Lys?Gly?Val?Glu?Cys?Leu?Gln?Ile?Glu?Gly

195?????????????????200?????????????????205Thr?Leu?Lys?Lys?Ser?Leu?Asp?Phe?Lys?Asn?Asp?Ile?Asn?Ala?Glu?Ala

210?????????????????215?????????????????220His?Ser?Trp?Gly?Met?Lys?Asn?Tyr?Glu?Glu?Trp?Ala?Lys?Asp?Leu?Thr225?????????????????230?????????????????235?????????????????240Asp?Ser?Gln?Arg?Glu?Ala?Leu?Asp?Gly?Tyr?Ala?Arg?Gln?Asp?Tyr?Lys

245?????????????????250?????????????????255Glu?Ile?Asn?Asn?Tyr?Leu?Arg?Asn?Gln?Gly?Gly?Ser?Gly?Asn?Glu?Lys

260?????????????????265?????????????????270Leu?Asp?Ala?Gln?Ile?Lys?Asn?Ile?Ser?Asp?Ala?Leu?Gly?Lys?Lys?Pro

275?????????????????280?????????????????285Ile?Pro?Glu?Asn?Ile?Thr?Val?Tyr?Arg?Trp?Cys?Gly?Met?Pro?Glu?Phe

290?????????????????295?????????????????300Gly?Tyr?Gln?Ile?Ser?Asp?Pro?Leu?Pro?Ser?Leu?Lys?Asp?Phe?Glu?Glu305?????????????????310?????????????????315?????????????????320Gln?Phe?Leu?Asn?Thr?Ile?Lys?Glu?Asp?Lys?Gly?Tyr?Met?Ser?Thr?Ser

325?????????????????330?????????????????335Leu?Ser?Ser?Glu?Arg?Leu?Ala?Ala?Phe?Gly?Ser?Arg?Lys?Ile?Ile?Leu

340?????????????????345?????????????????350Arg?Leu?Gln?Val?Pro?Lys?Gly?Ser?Thr?Gly?Ala?Tyr?Leu?Ser?Ala?Ile

355?????????????????360?????????????????365Gly?Gly?Phe?Ala?Ser?Glu?Lys?Glu?Ile?Leu?Leu?Asp?Lys?Asp?Ser?Lys???370??????????????????375?????????????????380Tyr?His?Ile?Asp?Lys?Val?Thr?Glu?Val?Ile?Ile?Lys?Gly?Val?Lys?Arg385?????????????????390?????????????????395?????????????????400Tyr?Val?Val?Asp?Ala?Thr?Leu?Leu?Thr?Asn

405?????????????????410

(2) about the information of SEQ ID NO:41

(ⅰ) sequence signature

(A) length: 72 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: other nucleic acid

(A) describe :/desc=" is used to make up the oligonucleotides of the coding eucaryon bio secretion signal of pCIB5527 "

(ⅲ) the false plan: not

(ⅹ ⅰ) sequence description: SEQ ID NO:41:GGATCCACCA TGGGCTGGAG CTGGATCTTC CTGTTCCTGC TGAGCGGCGC CGCGGGCGTG 60CACTGCCTGC AG 72

(2) about the information of SEQ ID NO:42

(ⅰ) sequence signature

(A) length: 1241 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: other nucleic acid

(A) describe :/desc=" synthetic DNA "

(ⅲ) the false plan: not

(ⅸ) feature:

(A) title/key: CDS

(B) position: 9..1238

(D) out of Memory :/remarks=" the corn optimization dna sequence dna of the eucaryote secretion signal of coding VIP2A (a) and bacillus secretion signal that cuts out and insertion is contained among the pCIB5528.”

(ⅹ ⅰ) sequence description: SEQ ID NO:42:GATCCACC ATG CTG CAG AAC CTG AAG ATC ACC GAC AAG GTG GAG GAC TTC 50

Met?Leu?Gln?Asn?Leu?Lys?Ile?Thr?Asp?Lys?Val?Glu?Asp?Phe

415?????????????????420AAG?GAG?GAC?AAG?GAG?AAG?GCC?AAG?GAG?TGG?GGC?AAG?GAG?AAG?GAG?AAG?????????98Lys?Glu?Asp?Lys?Glu?Lys?Ala?Lys?Glu?Trp?Gly?Lys?Glu?Lys?Glu?Lys425?????????????????430?????????????????435?????????????????440GAG?TGG?AAG?CTP?ACC?GCC?ACC?GAG?AAG?GGC?AAG?ATG?AAC?AAC?TTC?CTG????????146Glu?Trp?Lys?Leu?Thr?Ala?Thr?Glu?Lys?Gly?Lys?Met?Asn?Asn?Phe?Leu

445?????????????????450?????????????????455GAC?AAC?AAG?AAC?GAC?ATC?AAG?ACC?AAC?TAC?AAG?GAG?ATC?ACC?TTC?AGC????????194Asp?Asn?Lys?Asn?Asp?Ile?Lys?Thr?Asn?Tyr?Lys?Glu?Ile?Thr?Phe?Ser

460??????????????????465????????????????470ATA?GCC?GGC?AGC?TTC?GAG?GAC?GAG?ATC?AAG?GAC?CTG?AAG?GAG?ATC?GAC????????242Ile?Ala?Gly?Ser?Phe?Glu?Asp?Glu?Ile?Lys?Asp?Leu?Lys?Glu?Ile?Asp

475?????????????????480?????????????????485AAG?ATG?TTC?GAC?AAG?ACC?AAC?CTG?AGC?AAC?AGC?ATC?ATC?ACC?TAC?AAG????????290Lys?Met?Phe?Asp?Lys?Thr?Asn?Leu?Ser?Asn?Ser?Ile?Ile?Thr?Tyr?Lys

490?????????????????495?????????????????500AAC?GTG?GAG?CCC?ACC?ACC?ATC?GGC?TTC?AAC?AAG?AGC?CTG?ACC?GAG?GGC????????338Asn?Val?Glu?Pro?Thr?Thr?Ile?Gly?Phe?Asn?Lys?Ser?Leu?Thr?Glu?Gly505?????????????????510?????????????????515?????????????????520AAC?ACC?ATC?AAC?AGC?GAC?GCC?ATG?GCC?CAG?TTC?AAG?GAG?CAG?TTC?CTG????????386Asn?Thr?Ile?Asn?Ser?Asp?Ala?Met?Ala?Gln?Phe?Lys?Glu?Gln?Phe?Leu

525?????????????????530?????????????????535GAC?CGC?GAC?ATC?AAG?TTC?GAC?AGC?TAC?CTG?GAC?ACC?CAC?CTG?ACC?GCC????????434Asp?Arg?Asp?Ile?Lys?Phe?Asp?Ser?Tyr?Leu?Asp?Thr?His?Leu?Thr?Ala

540??????????????????545????????????????550CAG?CAG?GTG?AGC?AGC?AAG?GAG?CGC?GTG?ATC?CTG?AAG?GTG?ACC?GTC?CCC????????482Gln?Gln?Val?Ser?Ser?Lys?Glu?Arg?Val?Ile?Leu?Lys?Val?Thr?Val?Pro

555?????????????????560?????????????????565AGC?GGC?AAG?GGC?AGC?ACC?ACC?CCC?ACC?AAG?GCC?GGC?GTG?ATC?CTG?AAC????????530Ser?Gly?Lys?Gly?Ser?Thr?Thr?Pro?Thr?Lys?Ala?Gly?Val?Ile?Leu?Asn

570?????????????????575?????????????????580AAC?AGC?GAG?TAC?AAG?ATG?CTG?ATC?GAC?AAC?GGC?TAC?ATG?GTG?CAC?GTG????????578Asn?Ser?Glu?Tyr?Lys?Met?Leu?Ile?Asp?Asn?Gly?Tyr?Met?Val?His?Val585?????????????????590?????????????????595?????????????????600GAC?AAG?GTG?AGC?AAG?GTG?GTG?AAG?AAG?GGC?GTG?GAG?TGC?CTC?CAG?ATC????????626Asp?Lys?Val?Ser?Lys?Val?Val?Lys?Lys?Gly?Val?Glu?Cys?Leu?Gln?Ile

605?????????????????610?????????????????615GAG?GGC?ACC?CTG?AAG?AAG?AGT?CTA?GAC?TTC?AAG?AAC?GAC?ATC?AAC?GCC????????674Glu?Gly?Thr?Leu?Lys?Lys?Ser?Leu?Asp?Phe?Lys?Asn?Asp?Ile?Asn?Ala

620?????????????????625?????????????????630GAG?GCC?CAC?AGC?TGG?GGC?ATG?AAG?AAC?TAC?GAG?GAG?TGG?GCC?AAG?GAC????????722Glu?Ala?His?Ser?Trp?Gly?Met?Lys?Asn?Tyr?Glu?Glu?Trp?Ala?Lys?Asp

635?????????????????640?????????????????645CTG?ACC?GAC?AGC?CAG?CGC?GAG?GCC?CTG?GAC?GGC?TAC?GCC?CGC?CAG?GAC????????770Leu?Thr?Asp?Ser?Gln?Arg?Glu?Ala?Leu?Asp?Gly?Tyr?Ala?Arg?Gln?Asp

650?????????????????655?????????????????660TAC?AAG?GAG?ATC?AAC?AAC?TAC?CTG?CGC?AAC?CAG?GGC?GGC?AGC?GGC?AAC????????818Tyr?Lys?Glu?Ile?Asn?Asn?Tyr?Leu?Arg?Asn?Gln?Gly?Gly?Ser?Gly?Asn665?????????????????670?????????????????675?????????????????680GAG?AAG?CTG?GAC?GCC?CAG?ATC?AAG?AAC?ATC?AGC?GAC?GCC?CTG?GGC?AAG????????866Glu?Lys?Leu?Asp?Ala?Gln?Ile?Lys?Asn?Ile?Ser?Asp?Ala?Leu?Gly?Lys

685?????????????????690?????????????????695AAG?CCC?ATC?CCC?GAG?AAC?ATC?ACC?GTG?TAC?CGC?TGG?TGC?GGC?ATG?CCC????????914Lys?Pro?Ile?Pro?Glu?Asn?Ile?Thr?Val?Tyr?Arg?Trp?Cys?Gly?Met?Pro

700??????????????????705????????????????710GAG?TTC?GGC?TAC?CAG?ATC?AGC?GAC?CCC?CTG?CCC?AGC?CTG?AAG?GAC?TTC????????962Glu?Phe?Gly?Tyr?Gln?Ile?Ser?Asp?Pro?Leu?Pro?Ser?Leu?Lys?Asp?Phe

715?????????????????720?????????????????725

GAG?GAG?CAG?TTC?CTG?AAC?ACC?ATC?AAG?GAG?GAC?AAG?GGC?TAC?ATG?AGC???????1010Glu?Glu?Gln?Phe?Leu?Asn?Thr?Ile?Lys?Glu?Asp?Lys?Gly?Tyr?Met?Ser

730?????????????????735?????????????????740ACC?AGC?CTG?AGC?AGC?GAG?CGC?CTG?GCC?GCC?TTC?GGC?AGC?CGC?AAG?ATC???????1058Thr?Ser?Leu?Ser?Ser?Glu?Arg?Leu?Ala?Ala?Phe?Gly?Ser?Arg?Lys?Ile745?????????????????750?????????????????755?????????????????760ATC?CTG?CGC?CTG?CAG?GTG?CCC?AAG?GGC?AGC?ACT?GGT?GCC?TAC?CTG?AGC???????1106Ile?Leu?Arg?Leu?Gln?Val?Pro?Lys?Gly?Ser?Thr?Gly?Ala?Tyr?Leu?Ser

765?????????????????770?????????????????775GCC?ATC?GGC?GGC?TTC?GCC?AGC?GAG?AAG?GAG?ATC?CTG?CTG?GAT?AAG?GAC???????1154Ala?Ile?Gly?Gly?Phe?Ala?Ser?Glu?Lys?Glu?Ile?Leu?Leu?Asp?Lys?Asp

780?????????????????785?????????????????790AGC?AAG?TAC?CAC?ATC?GAC?AAG?GTG?ACC?GAG?GTG?ATC?ATC?AAG?GGC?GTG???????1202Ser?Lys?Tyr?His?Ile?Asp?Lys?Val?Thr?Glu?Val?Ile?Ile?Lys?Gly?Val

795?????????????????800?????????????????805AAG?CGC?TAC?GTG?GTG?GAC?GCC?ACC?CTG?CTG?ACC?AAC?TAG???????????????????1241Lys?Arg?Tyr?Val?Val?Asp?Ala?Thr?Leu?Leu?Thr?Asn

810?????????????????815?????????????????820

(2) about the information of SEQ ID NO:43

(ⅰ) sequence signature

(A) length: 410 amino acid

(B) type: amino acid

(D) topological structure: line style

(ⅱ) molecule type: protein

(ⅹ ⅸ) sequence description: SEQ ID NO:43:Met Leu Gln Asn Leu Lys Ile Thr Asp Lys Val Glu Asp Phe Lys Glu 15 10 15Asp Lys Glu Lys Ala Lys Glu Trp Gly Lys Glu Lys Glu Lys Glu Trp

20??????????????????25??????????????????30Lys?Leu?Thr?Ala?Thr?Glu?Lys?Gly?Lys?Met?Asn?Asn?Phe?Leu?Asp?Asn

35??????????????????40??????????????????45Lys?Asn?Asp?Ile?Lys?Thr?Ash?Tyr?Lys?Glu?Ile?Thr?Phe?Ser?Ile?Ala

50??????????????????55??????????????????60Gly?Ser?Phe?Glu?Asp?Glu?Ile?Lys?Asp?Leu?Lys?Glu?Ile?Asp?Lys?Met?65??????????????????70??????????????????75??????????????????80Phe?Asp?Lys?Thr?Asn?Leu?Ser?Asn?Ser?Ile?Ile?Thr?Tyr?Lys?Asn?Val

85??????????????????90??????????????????95Glu?Pro?Thr?Thr?Ile?Gly?Phe?Asn?Lys?Ser?Leu?Thr?Glu?Gly?Asn?Thr

100?????????????????105?????????????????110Ile?Asn?Ser?Asp?Ala?Met?Ala?Gln?Phe?Lys?Glu?Gln?Phe?Leu?Asp?Arg

115?????????????????120?????????????????125Asp?Ile?Lys?Phe?Asp?Ser?Tyr?Leu?Asp?Thr?His?Leu?Thr?Ala?Gln?Gln

130?????????????????135?????????????????140Val?Ser?Ser?Lys?Glu?Arg?Val?Ile?Leu?Lys?Val?Thr?Val?Pro?Ser?Gly145?????????????????150?????????????????155?????????????????160Lys?Gly?Ser?Thr?Thr?Pro?Thr?Lys?Ala?Gly?Val?Ile?Leu?Asn?Asn?Ser

165?????????????????170?????????????????175Glu?Tyr?Lys?Met?Leu?Ile?Asp?Asn?Gly?Tyr?Met?Val?His?Val?Asp?Lys

180?????????????????185?????????????????190Val?Ser?Lys?Val?Val?Lys?Lys?Gly?Val?Glu?Cys?Leu?Gln?Ile?Glu?Gly

195?????????????????200?????????????????205Thr?Leu?Lys?Lys?Ser?Leu?Asp?Phe?Lys?Asn?Asp?Ile?Asn?Ala?Glu?Ala

210?????????????????215?????????????????220His?Ser?Trp?Gly?Met?Lys?Asn?Tyr?Glu?Glu?Trp?Ala?Lys?Asp?Leu?Thr225?????????????????230?????????????????235?????????????????240Asp?Ser?Gln?Arg?Glu?Ala?Leu?Asp?Gly?Tyr?Ala?Arg?Gln?Asp?Tyr?Lys

245?????????????????250?????????????????255Glu?Ile?Asn?Asn?Tyr?Leu?Arg?Asn?Gln?Gly?Gly?Ser?Gly?Asn?Glu?Lys

260?????????????????265?????????????????270Leu?Asp?Ala?Gln?Ile?Lys?Asn?Ile?Ser?Asp?Ala?Leu?Gly?Lys?Lys?Pro

275?????????????????280?????????????????285Ile?Pro?Glu?Asn?Ile?Thr?Val?Tyr?Arg?Trp?Cys?Gly?Met?Pro?Glu?Phe

290?????????????????295?????????????????300Gly?Tyr?Gln?Ile?Ser?Asp?Pro?Leu?Pro?Ser?Leu?Lys?Asp?Phe?Glu?Glu305?????????????????310?????????????????315?????????????????320Gln?Phe?Leu?Asn?Thr?Ile?Lys?Glu?Asp?Lys?Gly?Tyr?Met?Ser?Thr?Ser

325?????????????????330?????????????????335Leu?Ser?Ser?Glu?Arg?Leu?Ala?Ala?Phe?Gly?Ser?Arg?Lys?Ile?Ile?Leu

340?????????????????345?????????????????350Arg?Leu?Gln?Val?Pro?Lys?Gly?Ser?Thr?Gly?Ala?Tyr?Leu?Ser?Ala?Ile

355?????????????????360?????????????????365Gly?Gly?Phe?Ala?Ser?Glu?Lys?Glu?Ile?Leu?Leu?Asp?Lys?Asp?Ser?Lys

370?????????????????375?????????????????380Tyr?His?Ile?Asp?Lys?Val?Thr?Glu?Val?Ile?Ile?Lys?Gly?Val?Lys?Arg385?????????????????390?????????????????395?????????????????400Tyr?Val?Val?Asp?Ala?Thr?Leu?Leu?Thr?Asn

405?????????????????410

(2) about the information of SEQ ID NO:44

(ⅰ) sequence signature

(A) length: 86 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: other nucleic acid

(A) describe :/desc=" is used to make up the oligonucleotides of the coding vacuole targeted peptide of pCIB5533 "

(ⅲ) the false plan: not

(ⅹ ⅰ) sequence description: SEQ ID NO:44:CCGCGGGCGT GCACTGCCTC AGCAGCAGCA GCTTCGCCGA CAGCAACCCC ATCCGCGTGA 60CCGACCGCGC CGCCAGCACC CTGCAG 86

(2) about the information of SEQ ID NO:45

(ⅰ) sequence signature

(A) length: 1358 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: other nucleic acid

(A) describe :/desc=" synthetic DNA "

(ⅲ) the false plan: not

(ⅸ) feature:

(A) title/key: CDS

(B) position: 9..1335

(D) out of Memory :/remarks=" the corn optimization dna sequence dna of the vacuole targeted peptide of coding VI P2A (a) and secreted from bacillus signal that cuts out and insertion is contained among the pCIB5533.”

(ⅹ ⅰ) sequence description: SEQ ID NO:45:GATCCACC ATG GGC TGG AGC TGG ATC TTC CTG TTC CTG CTG AGC GGC GCC 50

Met?Gly?Trp?Ser?Trp?Ile?Phe?Leu?Phe?Leu?Leu?Ser?Gly?Ala

415?????????????????420GCG?GGC?GTG?CAC?TGC?CTC?AGC?AGC?AGC?AGC?TTC?GCC?GAC?AGC?AAC?CCC?????????98Ala?Gly?Val?His?Cys?Leu?Ser?Ser?Ser?Ser?Phe?Ala?Asp?Ser?Asn?Pro425?????????????????430?????????????????435?????????????????440ATC?CGC?GTG?ACC?GAC?CGC?GCC?GCC?AGC?ACC?CTG?CAG?AAC?CTG?AAG?ATC????????146Ile?Arg?Val?Thr?Asp?Arg?Ala?Ala?Ser?Thr?Leu?Gln?Asn?Leu?Lys?Ile

445?????????????????450?????????????????455ACC?GAC?AAG?GTG?GAG?GAC?TTC?AAG?GAG?GAC?AAG?GAG?AAG?GCC?AAG?GAG????????194Thr?Asp?Lys?Val?Glu?Asp?Phe?Lys?Glu?Asp?Lys?Glu?Lys?Ala?Lys?Glu

460?????????????????465?????????????????470TGG?GGC?AAG?GAG?AAG?GAG?AAG?GAG?TGG?AAG?CTT?ACC?GCC?ACC?GAG?AAG????????242Trp?Gly?Lys?Glu?Lys?Glu?Lys?Glu?Trp?Lys?Leu?Thr?Ala?Thr?Glu?Lys

475?????????????????480?????????????????485GGC?AAG?ATG?AAC?AAC?TTC?CTG?GAC?AAC?AAG?AAC?GAC?ATC?AAG?ACC?AAC?????????290Gly?Lys?Met?Asn?Asn?Phe?Leu?Asp?Asn?Lys?Asn?Asp?Ile?Lys?Thr?Asn

490?????????????????495?????????????????500TAC?AAG?GAG?ATC?ACC?TTC?AGC?ATA?GCC?GGC?AGC?TTC?GAG?GAC?GAG?ATC?????????338Tyr?Lys?Glu?Ile?Thr?Phe?Ser?Ile?Ala?Gly?Ser?Phe?Glu?Asp?Glu?Ile505?????????????????510?????????????????515?????????????????520AAG?GAC?CTG?AAG?GAG?ATC?GAC?AAG?ATG?TTC?GAC?AAG?ACC?AAC?CTG?AGC?????????386Lys?Asp?Leu?Lys?Glu?Ile?Asp?Lys?Met?Phe?Asp?Lys?Thr?Asn?Leu?Ser

525?????????????????530?????????????????535AAC?AGC?ATC?ATC?ACC?TAC?AAG?AAC?GTG?GAG?CCC?ACC?ACC?ATC?GGC?TTC?????????434Asn?Ser?Ile?Ile?Thr?Tyr?Lys?Asn?Val?Glu?Pro?Thr?Thr?Ile?Gly?Phe

540?????????????????545?????????????????550AAC?AAG?AGC?CTG?ACC?GAG?GGC?AAC?ACC?ATC?AAC?AGC?GAC?GCC?ATG?GCC?????????482Asn?Lys?Ser?Leu?Thr?Glu?Gly?Asn?Thr?Ile?Asn?Ser?Asp?Ala?Met?Ala

555?????????????????560?????????????????565CAG?TTC?AAG?GAG?CAG?TTC?CTG?GAC?CGC?GAC?ATC?AAG?TTC?GAC?AGC?TAC?????????530Gln?Phe?Lys?Glu?Gln?Phe?Leu?Asp?Arg?Asp?Ile?Lys?Phe?Asp?Ser?Tyr

570?????????????????575?????????????????580CTG?GAC?ACC?CAC?CTG?ACC?GCC?CAG?CAG?GTG?AGC?AGC?AAG?GAG?CGC?GTG?????????578Leu?Asp?Thr?His?Leu?Thr?Ala?Gln?Gln?Val?Ser?Ser?Lys?Glu?Arg?Val585?????????????????590?????????????????595?????????????????600ATC?CTG?AAG?GTG?ACC?GTC?CCC?AGC?GGC?AAG?GGC?AGC?ACC?ACC?CCC?ACC?????????626Ile?Leu?Lys?Val?Thr?Val?Pro?Ser?Gly?Lys?Gly?Ser?Thr?Thr?Pro?Thr

605?????????????????610?????????????????615AAG?GCC?GGC?GTG?ATC?CTG?AAC?AAC?AGC?GAG?TAC?AAG?ATG?CTG?ATC?GAC?????????674Lys?Ala?Gly?Val?Ile?Leu?Asn?Asn?Ser?Glu?Tyr?Lys?Met?Leu?Ile?Asp

620?????????????????625?????????????????630AAC?GGC?TAC?ATG?GTG?CAC?GTG?GAC?AAG?GTG?AGC?AAG?GTG?GTG?AAG?AAG?????????722Asn?Gly?Tyr?Met?Val?His?Val?Asp?Lys?Val?Ser?Lys?Val?Val?Lys?Lys

635?????????????????640?????????????????645GGC?GTG?GAG?TGC?CTC?CAG?ATC?GAG?GGC?ACC?CTG?AAG?AAG?AGT?CTA?GAC?????????770Gly?Val?Glu?Cys?Leu?Gln?Ile?Glu?Gly?Thr?Leu?Lys?Lys?Ser?Leu?Asp

650?????????????????655?????????????????660TTC?AAG?AAC?GAC?ATC?AAC?GCC?GAG?GCC?CAC?AGC?TGG?GGC?ATG?AAG?AAC?????????818Phe?Lys?Asn?Asp?Ile?Asn?Ala?Glu?Ala?His?Ser?Trp?Gly?Met?Lys?Asn665????????????????670??????????????????675?????????????????680TAC?GAG?GAG?TGG?GCC?AAG?GAC?CTG?ACC?GAC?AGC?CAG?CGC?GAG?GCC?CTG?????????866Tyr?Glu?Glu?Trp?Ala?Lys?Asp?Leu?Thr?Asp?Ser?Gln?Arg?Glu?Ala?Leu

685?????????????????690?????????????????695GAC?GGC?TAC?GCC?CGC?CAG?GAC?TAC?AAG?GAG?ATC?AAC?AAC?TAC?CTG?CGC?????????914Asp?Gly?Tyr?Ala?Arg?Gln?Asp?Tyr?Lys?Glu?Ile?Asn?Asn?Tyr?Leu?Arg

700?????????????????705?????????????????710AAC?CAG?GGC?GGC?AGC?GGC?AAC?GAG?AAG?CTG?GAC?GCC?CAG?ATC?AAG?AAC?????????962Asn?Gln?Gly?Gly?Ser?Gly?Asn?Glu?Lys?Leu?Asp?Ala?Gln?Ile?Lys?Asn

715?????????????????720?????????????????725ATC?AGC?GAC?GCC?CTG?GGC?AAG?AAG?CCC?ATC?CCC?GAG?AAC?ATC?ACC?GTG???????1010Ile?Ser?Asp?Ala?Leu?Gly?Lys?Lys?Pro?Ile?Pro?Glu?Asn?Ile?Thr?Val

730?????????????????735?????????????????740TAC?CGC?TGG?TGC?GGC?ATG?CCC?GAG?TTC?GGC?TAC?CAG?ATC?AGC?GAC?CCC???????1058Tyr?Arg?Trp?Cys?Gly?Met?Pro?Glu?Phe?Gly?Tyr?Gln?Ile?Ser?Asp?Pro745?????????????????750?????????????????755?????????????????760CTG?CCC?AGC?CTG?AAG?GAC?TTC?GAG?GAG?CAG?TTC?CTG?AAC?ACC?ATC?AAG???????1106Leu?Pro?Ser?Leu?Lys?Asp?Phe?Glu?Glu?Gln?Phe?Leu?Asn?Thr?Ile?Lys

765?????????????????770?????????????????775GAG?GAC?AAG?GGC?TAC?ATG?AGC?ACC?AGC?CTG?AGC?AGC?GAG?CGC?CTG?GCC???????1154Glu?Asp?Lys?Gly?Tyr?Met?Ser?Thr?Ser?Leu?Ser?Ser?Glu?Arg?Leu?Ala

780?????????????????785?????????????????790GCC?TTC?GGC?AGC?CGC?AAG?ATC?ATC?CTG?CGC?CTG?CAG?GTG?CCC?AAG?GGC???????1202Ala?Phe?Gly?Ser?Arg?Lys?Ile?Ile?Leu?Arg?Leu?Gln?Val?Pro?Lys?Gly

795??????????????????800????????????????805AGC?ACT?GGT?GCC?TAC?CTG?AGC?GCC?ATC?GGC?GGC?TTC?GCC?AGC?GAG?AAG???????1250Ser?Thr?Gly?Ala?Tyr?Leu?Ser?Ala?Ile?Gly?Gly?Phe?Ala?Ser?Glu?Lys

810?????????????????815?????????????????820GAG?ATC?CTG?CTG?GAT?AAG?GAC?AGC?AAG?TAC?CAC?ATC?GAC?AAG?GTG?ACC???????1298Glu?Ile?Leu?Leu?Asp?Lys?Asp?Ser?Lys?Tyr?His?Ile?Asp?Lys?Val?Thr825?????????????????830?????????????????835?????????????????840GAG?GTG?ATC?ATC?AAG?GGC?GTG?AAG?CGC?TAC?GTG?GTG?GAC?GCC?ACC?CTG???????1346Glu?Val?Ile?Ile?Lys?Gly?Val?Lys?Arg?Tyr?Val?Val?Asp?Ala?Thr?Leu

845?????????????????850?????????????????855CTG?ACC?AAC?TAG???????????????????????????????????????????????????????1358Leu?Thr?Asn

(2) about the information of SEQ ID NO:46

(ⅰ) sequence signature

(A) length: 449 amino acid

(B) type: amino acid

(D) topological structure: line style

(ⅱ) molecule type: protein

(ⅹ ⅰ) sequence description: SEQ ID NO:46:Met Gly Trp Ser Trp Ile Phe Leu Phe Leu Leu Ser Gly Ala Ala Gly 15 10 15Val His Cys Leu Ser Ser Ser Ser Phe Ala Asp Ser Asn Pro Ile Arg

20??????????????????25??????????????????30Val?Thr?Asp?Arg?Ala?Ala?Ser?Thr?Leu?Gln?Asn?Leu?Lys?Ile?Thr?Asp

35??????????????????40??????????????????45Lys?Val?Glu?Asp?Phe?Lys?Glu?Asp?Lys?Glu?Lys?Ala?Lys?Glu?Trp?Gly

50??????????????????55??????????????????60Lys?Glu?Lys?Glu?Lys?Glu?Trp?Lys?Leu?Thr?Ala?Thr?Glu?Lys?Gly?Lys?65??????????????????70??????????????????75??????????????????80Met?Asn?Asn?Phe?Leu?Asp?Asn?Lys?Asn?Asp?Ile?Lys?Thr?Asn?Tyr?Lys

85??????????????????90??????????????????95Glu?Ile?Thr?Phe?Ser?Ile?Ala?Gly?Ser?Phe?Glu?Asp?Glu?Ile?Lys?Asp

100?????????????????105?????????????????110Leu?Lys?Glu?Ile?Asp?Lys?Met?Phe?Asp?Lys?Thr?Asn?Leu?Ser?Asn?Ser

115?????????????????120?????????????????125Ile?Ile?Thr?Tyr?Lys?Asn?Val?Glu?Pro?Thr?Thr?Ile?Gly?Phe?Asn?Lys

130?????????????????135?????????????????140Ser?Leu?Thr?Glu?Gly?Asn?Thr?Ile?Asn?Ser?Asp?Ala?Met?Ala?Gln?Phe145?????????????????150?????????????????155?????????????????160Lys?Glu?Gln?Phe?Leu?Asp?Arg?Asp?Ile?Lys?Phe?Asp?Ser?Tyr?Leu?Asp

165?????????????????170?????????????????175Thr?His?Leu?Thr?Ala?Gln?Gln?Val?Ser?Ser?Lys?Glu?Arg?Val?Ile?Leu

180?????????????????185?????????????????190Lys?Val?Thr?Val?Pro?Ser?Gly?Lys?Gly?Ser?Thr?Thr?Pro?Thr?Lys?Ala

195?????????????????200?????????????????205Gly?Val?Ile?Leu?Asn?Asn?Ser?Glu?Tyr?Lys?Met?Leu?Ile?Asp?Asn?Gly

210?????????????????215?????????????????220Tyr?Met?Val?His?Val?Asp?Lys?Val?Ser?Lys?Val?Val?Lys?Lys?Gly?Val225?????????????????230?????????????????235?????????????????240Glu?Cys?Leu?Gln?Ile?Glu?Gly?Thr?Leu?Lys?Lys?Ser?Leu?Asp?Phe?Lys

245?????????????????250?????????????????255Asn?Asp?Ile?Asn?Ala?Glu?Ala?His?Ser?Trp?Gly?Met?Lys?Asn?Tyr?Glu

260?????????????????265?????????????????270Glu?Trp?Ala?Lys?Asp?Leu?Thr?Asp?Ser?Gln?Arg?Glu?Ala?Leu?Asp?Gly

275?????????????????280?????????????????285Tyr?Ala?Arg?Gln?Asp?Tyr?Lys?Glu?Ile?Asn?Asn?Tyr?Leu?Arg?Asn?Gln

290?????????????????295?????????????????300Gly?Gly?Ser?Gly?Asn?Glu?Lys?Leu?Asp?Ala?Gln?Ile?Lys?Asn?Ile?Ser305?????????????????310?????????????????315?????????????????320Asp?Ala?Leu?Gly?Lys?Lys?Pro?Ile?Pro?Glu?Asn?Ile?Thr?Val?Tyr?Arg

325?????????????????330?????????????????335TrpCys?Gly?Met?Pro?Glu?Phe?Gly?Tyr?Gln?Ile?Ser?Asp?Pro?Leu?Pro

340?????????????????345?????????????????350SerLeu?Lys?Asp?Phe?Glu?Glu?Gln?Phe?Leu?Asn?Thr?Ile?Lys?Glu?Asp

355?????????????????360?????????????????365Lys?Gly?Tyr?Met?Ser?Thr?Ser?Leu?Ser?Ser?Glu?Arg?Leu?Ala?Ala?Phe

370?????????????????375?????????????????380Gly?Ser?Arg?Lys?Ile?Ile?Leu?Arg?Leu?Gln?Val?Pro?Lys?Gly?Ser?Thr385?????????????????390?????????????????395?????????????????400Gly?Ala?Tyr?Leu?Ser?Ala?Ile?Gly?Gly?Phe?Ala?Ser?Glu?Lys?Glu?Ile

405?????????????????410?????????????????415Leu?Leu?Asp?Lys?Asp?Ser?Lys?Tyr?His?Ile?Asp?Lys?Val?Thr?Glu?Val

420?????????????????425?????????????????430IleIle?Lys?Gly?Val?Lys?Arg?Tyr?Val?Val?Asp?Ala?Thr?Leu?Leu?Thr

435?????????????????440?????????????????445Asn

(2) about the information of SEQ ID NO:47

(ⅰ) sequence signature

(A) length: 16 amino acid

(B) type: amino acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: peptide

(ⅲ) the false plan: not.

(ⅸ) feature:

(A) title/key: peptide

(B) position: 1..16

(D) out of Memory: the connection peptides of/remarks=" be used to make up pCIB5533, merge VIP1A (a) and VIP2A (a) "

(ⅹ ⅰ) sequence description: SEQ ID NO:47:Pro Ser Thr Pro Pro Thr Pro Ser Pro Ser Thr Pro Pro Thr Pro Ser1 5 10 15

(2) about the information of SEQ ID NO:48

(ⅰ) sequence signature

(A) length: 66 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: other nucleic acid

(A) describe :/desc=" is used to make up the connection peptides DNA of pCIB553 "

(ⅲ) the false plan: not

(ⅹ ⅰ) sequence description: SEQ ID NO:48:CCCGGGCCTT CTACTCCCCC AACTCCCTCT CCTAGCACGC CTCCGACACC TAGCGATATC 60GGATCC 66

(2) about the information of SEQ ID NO:49

(ⅰ) sequence signature

(A) length: 4031 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: other nucleic acid

(A) describe :/desc=" synthetic DNA "

(ⅲ) the false plan: not

(ⅸ) feature:

(A) title/key: CDS

(B) position: 6..4019

(D) out of Memory :/remarks=" the corn optimization dna sequence dna of coding VIP2A (a)-VIP1A (a) fusion is contained among the pCIB5531.”

(ⅹ ⅰ) sequence description: SEQ ID NO:49:GATCC ATG AAG CGC ATG GAG GGC AAG CTG TTC ATG GTG AGC AAG AAG 47

Met?Lys?Arg?Met?Glu?Gly?Lys?Leu?Phe?Met?Val?Ser?Lys?Lys

450?????????????????455?????????????????460CTC?CAG?GTG?GTG?ACC?AAG?ACC?GTG?CTG?CTG?AGC?ACC?GTG?TTC?AGC?ATC?????????95Leu?Gln?Val?Val?Thr?Lys?Thr?Val?Leu?Leu?Ser?Thr?Val?Phe?Ser?Ile

465???????????????470?????????????????475AGC?CTG?CTG?AAC?AAC?GAG?GTG?ATC?AAG?GCC?GAG?CAG?CTG?AAC?ATC?AAC?????????143Ser?Leu?Leu?Asn?Asn?Glu?Val?Ile?Lys?Ala?Glu?Gln?Leu?Asn?Ile?Asn480?????????????????485?????????????????490?????????????????495AGC?CAG?AGC?AAG?TAC?ACC?AAC?CTC?CAG?AAC?CTG?AAG?ATC?ACC?GAC?AAG?????????191Ser?Gln?Ser?Lys?Tyr?Thr?Asn?Leu?Gln?Asn?Leu?Lys?Ile?Thr?Asp?Lys

500?????????????????505?????????????????510GTG?GAG?GAC?TTC?AAG?GAG?GAC?AAG?GAG?AAG?GCC?AAG?GAG?TGG?GGC?AAG?????????239Val?Glu?Asp?Phe?Lys?Glu?Asp?Lys?Glu?Lys?Ala?Lys?Glu?Trp?Gly?Lys

515?????????????????520??????????????????525GAG?AAG?GAG?AAG?GAG?TGG?AAG?CTT?ACC?GCC?ACC?GAG?AAG?GGC?AAG?ATG?????????287Glu?Lys?Glu?Lys?Glu?Trp?Lys?Leu?Thr?Ala?Thr?Glu?Lys?Gly?Lys?Met

530?????????????????535?????????????????540AAC?AAC?TTC?CTG?GAC?AAC?AAG?AAC?GAC?ATC?AAG?ACC?AAC?TAC?AAG?GAG?????????335Asn?Asn?Phe?Leu?Asp?Asn?Lys?Asn?Asp?Ile?Lys?Thr?Asn?Tyr?Lys?Glu

545?????????????????550??????????????????555ATC?ACC?TTC?AGC?ATA?GCC?GGC?AGC?TTC?GAG?GAC?GAG?ATC?AAG?GAC?CTG?????????383Ile?Thr?Phe?Ser?Ile?Ala?Gly?Ser?Phe?Glu?Asp?Glu?Ile?Lys?Asp?Leu560?????????????????565?????????????????570?????????????????575AAG?GAG?ATC?GAC?AAG?ATG?TTC?GAC?AAG?ACC?AAC?CTG?AGC?AAC?AGC?ATC?????????431Lys?Glu?Ile?Asp?Lys?Met?Phe?Asp?Lys?Thr?Asn?Leu?Ser?Asn?Ser?Ile

580?????????????????585?????????????????590ATC?ACC?TAC?AAG?AAC?GTG?GAG?CCC?ACC?ACC?ATC?GGC?TTC?AAC?AAG?AGC?????????479Ile?Thr?Tyr?Lys?Asn?Val?Glu?Pro?Thr?Thr?Ile?Gly?Phe?Asn?Lys?Ser

595?????????????????600?????????????????605CTG?ACC?GAG?GGC?AAC?ACC?ATC?AAC?AGC?GAC?GCC?ATG?GCC?CAG?TTC?AAG?????????527Leu?Thr?Glu?Gly?Asn?Thr?Ile?Asn?Ser?Asp?Ala?Met?Ala?Gln?Phe?Lys

610?????????????????615?????????????????620GAG?CAG?TTC?CTG?GAC?CGC?GAC?ATC?AAG?TTC?GAC?AGC?TAC?CTG?GAC?ACC?????????575Glu?Gln?Phe?Leu?Asp?Arg?Asp?Ile?Lys?Phe?Asp?Ser?Tyr?Leu?Asp?Thr

625?????????????????630?????????????????635CAC?CTG?ACC?GCC?CAG?CAG?GTG?AGC?AGC?AAG?GAG?CGC?GTG?ATC?CTG?AAG?????????623His?Leu?Thr?Ala?Gln?Gln?Val?Ser?Ser?Lys?Glu?Arg?Val?Ile?Leu?Lys640?????????????????645?????????????????650?????????????????655GTG?ACC?GTC?CCC?AGC?GGC?AAG?GGC?AGC?ACC?ACC?CCC?ACC?AAG?GCC?GGC?????????671Val?Thr?Val?Pro?Ser?Gly?Lys?Gly?Ser?Thr?Thr?Pro?Thr?Lys?Ala?Gly

660?????????????????665?????????????????670GTG?ATC?CTG?AAC?AAC?AGC?GAG?TAC?AAG?ATG?CTG?ATC?GAC?AAC?GGC?TAC?????????719Val?Ile?Leu?Asn?Asn?Ser?Glu?Tyr?Lys?Met?Leu?Ile?Asp?Asn?Gly?Tyr

675?????????????????680?????????????????685ATG?GTG?CAC?GTG?GAC?AAG?GTG?AGC?AAG?GTG?GTG?AAG?AAG?GGC?GTG?GAG?????????767Met?Val?His?Val?Asp?Lys?Val?Ser?Lys?Val?Val?Lys?Lys?Gly?Val?Glu

690?????????????????695?????????????????700TGC?CTC?CAG?ATC?GAG?GGC?ACC?CTG?AAG?AAG?AGT?CTA?GAC?TTC?AAG?AAC?????????815Cys?Leu?Gln?Ile?Glu?Gly?Thr?Leu?Lys?Lys?Ser?Leu?Asp?Phe?Lys?Asn

705?????????????????710?????????????????715GAC?ATC?AAC?GCC?GAG?GCC?CAC?AGC?TGG?GGC?ATG?AAG?AAC?TAC?GAG?GAG?????????863Asp?Ile?Asn?Ala?Glu?Ala?His?Ser?Trp?Gly?Met?Lys?Asn?Tyr?Glu?Glu720?????????????????725?????????????????730?????????????????735TGG?GCC?AAG?GAC?CTG?ACC?GAC?AGC?CAG?CGC?GAG?GCC?CTG?GAC?GGC?TAC?????????911Trp?Ala?Lys?Asp?Leu?Thr?Asp?Ser?Gln?Arg?Glu?Ala?Leu?Asp?Gly?Tyr

740?????????????????745?????????????????750GCC?CGC?CAG?GAC?TAC?AAG?GAG?ATC?AAC?AAC?TAC?CTG?CGC?AAC?CAG?GGC?????????959Ala?Arg?Gln?Asp?Tyr?Lys?Glu?Ile?Asn?Asn?Tyr?Leu?Arg?Asn?Gln?Gly

755?????????????????760?????????????????765GGC?AGC?GGC?AAC?GAG?AAG?CTG?GAC?GCC?CAG?ATC?AAG?AAC?ATC?AGC?GAC????????1007Gly?Ser?Gly?Asn?Glu?Lys?Leu?Asp?Ala?Gln?Ile?Lys?Asn?Ile?Ser?Asp

770?????????????????775?????????????????780GCC?CTG?GGC?AAG?AAG?CCC?ATC?CCC?GAG?AAC?ATC?ACC?GTG?TAC?CGC?TGG????????1055Ala?Leu?Gly?Lys?Lys?Pro?Ile?Pro?Glu?Asn?Ile?Thr?Val?Tyr?Arg?Trp

785?????????????????790?????????????????795TGC?GGC?ATG?CCC?GAG?TTC?GGC?TAC?CAG?ATC?AGC?GAC?CCC?CTG?CCC?AGC????????1103Cys?Gly?Met?Pro?Glu?Phe?Gly?Tyr?Gln?Ile?Ser?Asp?Pro?Leu?Pro?Ser800?????????????????805?????????????????810?????????????????815CTG?AAG?GAC?TTC?GAG?GAG?CAG?TTC?CTG?AAC?ACC?ATC?AAG?GAG?GAC?AAG????????1151Leu?Lys?Asp?Phe?Glu?Glu?Gln?Phe?Leu?Asn?Thr?Ile?Lys?Glu?Asp?Lys

820?????????????????825?????????????????830GGC?TAC?ATG?AGC?ACC?AGC?CTG?AGC?AGC?GAG?CGC?CTG?GCC?GCC?TIC?GGC????????1199Gly?Tyr?Met?Ser?Thr?Ser?Leu?Ser?Ser?Glu?Arg?Leu?Ala?Ala?Phe?Gly

835?????????????????840?????????????????845AGC?CGC?AAG?ATC?ATC?CTG?CGC?CTG?CAG?GTG?CCC?AAG?GGC?AGC?ACT?GGT????????1247Ser?Arg?Lys?Ile?Ile?Leu?Arg?Leu?Gln?Val?Pro?Lys?Gly?Ser?Thr?Gly

850?????????????????855?????????????????860GCC?TAC?CTG?AGC?GCC?ATC?GGC?GGC?TIC?GCC?AGC?GAG?AAG?GAG?ATC?CTG????????1295Ala?Tyr?Leu?Ser?Ala?Ile?Gly?Gly?Phe?Ala?Ser?Glu?Lys?Glu?Ile?Leu

865?????????????????870?????????????????875CTG?GAT?AAG?GAC?AGC?AAG?TAC?CAC?ATC?GAC?AAG?GTG?ACC?GAG?GTG?ATC????????1343Leu?Asp?Lys?Asp?Ser?Lys?Tyr?His?Ile?Asp?Lys?Val?Thr?Glu?Val?Ile880?????????????????885?????????????????890?????????????????895ATC?AAG?GGC?GTG?AAG?CGC?TAC?GTG?GTG?GAC?GCC?ACC?CTG?CTG?ACC?AAC????????1391Ile?Lys?Gly?Val?Lys?Arg?Tyr?Val?Val?Asp?Ala?Thr?Leu?Leu?Thr?Asn

900?????????????????905?????????????????910TCC?CGG?GGG?CCT?TCT?ACT?CCC?CCA?ACT?CCC?TCT?CCT?AGC?ACG?CCT?CCG????????1439Ser?Arg?Gly?Pro?Ser?Thr?Pro?Pro?Thr?Pro?Ser?Pro?Ser?Thr?Pro?Pro

915?????????????????920?????????????????925ACA?CCT?AGC?GAT?ATC?GGA?TCC?ACC?ATG?AAG?ACC?AAC?CAG?ATC?AGC?ACC????????1487Thr?Pro?Ser?Asp?Ile?Gly?Ser?Thr?Met?Lys?Thr?Asn?Gln?Ile?Ser?Thr

930?????????????????935?????????????????940ACC?CAG?AAG?AAC?CAG?CAG?AAG?GAG?ATG?GAC?CGC?AAG?GGC?CTG?CTG?GGC???????1535Thr?Gln?Lys?Asn?Gln?Gln?Lys?Glu?Met?Asp?Arg?Lys?Gly?Leu?Leu?Gly

945?????????????????950?????????????????955TAC?TAC?TTC?AAG?GGC?AAG?GAC?TTC?AGC?AAC?CTG?ACC?ATG?TTC?GCC?CCC???????1583Tyr?Tyr?Phe?Lys?Gly?Lys?Asp?Phe?Ser?Asn?Leu?Thr?Met?Phe?Ala?Pro960?????????????????965?????????????????970?????????????????975ACG?CGT?GAC?AGC?ACC?CTG?ATC?TAC?GAC?CAG?CAG?ACC?GCC?AAC?AAG?CTG???????1631Thr?Arg?Asp?Ser?Thr?Leu?Ile?Tyr?Asp?Gln?Gln?Thr?Ala?Asn?Lys?Leu

980?????????????????985?????????????????990CTG?GAC?AAG?AAG?CAG?CAG?GAG?TAC?CAG?AGC?ATC?CGC?TGG?ATC?GGC?CTG???????1679Leu?Asp?Lys?Lys?Gln?Gln?Glu?Tyr?Gln?Ser?Ile?Arg?Trp?Ile?Gly?Leu

995?????????????????1000????????????????1005ATC?CAG?AGC?AAG?GAG?ACC?GGC?GAC?TTC?ACC?TTC?AAC?CTG?AGC?GAG?GAC???????1727Ile?Gln?Ser?Lys?Glu?Thr?Gly?Asp?Phe?Thr?Phe?Asn?Leu?Ser?Glu?Asp

1010????????????????1015????????????????1020GAG?CAG?GCC?ATC?ATC?GAG?ATC?AAC?GGC?AAG?ATC?ATC?AGC?AAC?AAG?GGC???????1775Glu?Gln?Ala?Ile?Ile?Glu?Ile?Asn?Gly?Lys?Ile?Ile?Ser?Asn?Lys?Gly

1025????????????????1030????????????????1035AAG?GAG?AAG?CAG?GTG?GTG?CAC?CTG?GAG?AAG?GGC?AAG?CTG?GTG?CCC?ATC???????1823Lys?Glu?Lys?Gln?Val?Val?His?Leu?Glu?Lys?Gly?Lys?Leu?Val?Pro?Ile1040????????????????1045????????????????1050????????????????1055AAG?ATC?GAG?TAC?CAG?AGC?GAC?ACC?AAG?TTC?AAC?ATC?GAC?AGC?AAG?ACC???????1871Lys?Ile?Glu?Tyr?Gln?Ser?Asp?Thr?Lys?Phe?Asn?Ile?Asp?Ser?Lys?Thr

1060????????????????1065????????????????1070TTC?AAG?GAG?CTG?AAG?CTT?TTC?AAG?ATC?GAC?AGC?CAG?AAC?CAG?CCC?CAG???????1919Phe?Lys?Glu?Leu?Lys?Leu?Phe?Lys?Ile?Asp?Ser?Gln?Asn?Gln?Pro?Gln

1075????????????????1080????????????????1085CAG?GTG?CAG?CAG?GAC?GAG?CTG?CGC?AAC?CCC?GAG?TTC?AAC?AAG?AAG?GAG???????1967Gln?Val?Gln?Gln?Asp?Glu?Leu?Arg?Asn?Pro?Glu?Phe?Asn?Lys?Lys?Glu

1090????????????????1095????????????????1100AGC?CAG?GAG?TIC?CTG?GCC?AAG?CCC?AGC?AAG?ATC?AAC?CTG?TIC?ACC?CAG???????2015Ser?Gln?Glu?Phe?Leu?Ala?Lys?Pro?Ser?Lys?Ile?Asn?Leu?Phe?Thr?Gln

1105????????????????1110????????????????1115CAG?ATG?AAG?CGC?GAG?ATC?GAC?GAG?GAC?ACC?GAC?ACC?GAC?GGC?GAC?AGC???????2063Gln?Met?Lys?Arg?Glu?Ile?Asp?Glu?Asp?Thr?Asp?Thr?Asp?Gly?Asp?Ser1120????????????????1125????????????????1130????????????????1135ATC?CCC?GAC?CTG?TGG?GAG?GAG?AAC?GGC?TAC?ACC?ATC?CAG?AAC?CGC?ATC???????2111Ile?Pro?Asp?Leu?Trp?Glu?Glu?Asn?Gly?Tyr?Thr?Ile?Gln?Asn?Arg?Ile

1140?????????????????1145???????????????1150GCC?GTG?AAG?TGG?GAC?GAC?AGC?CTG?GCT?AGC?AAG?GGC?TAC?ACC?AAG?TTC???????2159AAa?Val?Lys?Trp?Asp?Asp?Ser?Leu?Ala?Ser?Lys?Gly?Tyr?Thr?Lys?Phe

1155???????????????1160????????????????1165GTG?AGC?AAC?CCC?CTG?GAG?AGC?CAC?ACC?GTG?GGC?GAC?CCC?TAC?ACC?GAC???????2207Val?Ser?Asn?Pro?Leu?Glu?Ser?His?Thr?Val?Gly?Asp?Pro?Tyr?Thr?Asp

1170????????????????1175????????????????1180TAC?GAG?AAG?GCC?GCC?CGC?GAC?CTG?GAC?CTG?AGC?AAC?GCC?AAG?GAG?ACC???????2255Tyr?Glu?Lys?Ala?Ala?Arg?Asp?Leu?Asp?Leu?Ser?Asn?Ala?Lys?Glu?Thr

1185????????????????1190????????????????1195TTC?AAC?CCC?CTG?GTG?GCC?GCC?TTC?CCC?AGC?GTG?AAC?GTG?AGC?ATG?GAG???????2303Phe?Asn?Pro?Leu?Val?Ala?Ala?Phe?Pro?Ser?Val?Asn?Val?Ser?Met?Glu1200????????????????1205????????????????1210????????????????1215AAG?GTG?ATC?CTG?AGC?CCC?AAC?GAG?AAC?CTG?AGC?AAC?AGC?GTG?GAG?AGC???????2351Lys?Val?Ile?Leu?Ser?Pro?Asn?Glu?Asn?Leu?Ser?Asn?Ser?Val?Glu?Ser

1220????????????????1225????????????????1230CAC?TCG?AGC?ACC?AAC?TGG?AGC?TAC?ACC?AAC?ACC?GAG?GGC?GCC?AGC?GTG???????2399His?Ser?Ser?Thr?Asn?Trp?Ser?Tyr?Thr?Asn?Thr?Glu?Gly?Ala?Ser?Val

1235????????????????1240????????????????1245GAG?GCC?GGC?ATC?GGT?CCC?AAG?GGC?ATC?AGC?TTC?GGC?GTG?AGC?GTG?AAC???????2447Glu?Ala?Gly?Ile?Gly?Pro?Lys?Gly?Ile?Ser?Phe?Gly?Val?Ser?Val?Asn

1250????????????????1255????????????????1260TAC?CAG?CAC?AGC?GAG?ACC?GTG?GCC?CAG?GAG?TGG?GGC?ACC?AGC?ACC?GGC???????2495Tyr?Gln?His?Ser?Glu?Thr?Val?Ala?Gln?Glu?Trp?Gly?Thr?Ser?Thr?Gly

1265????????????????1270????????????????1275AAC?ACC?AGC?CAG?TTC?AAC?ACC?GCC?AGC?GCC?GGC?TAC?CTG?AAC?GCC?AAC???????2543Asn?Thr?Ser?Gln?Phe?Asn?Thr?Ala?Ser?Ala?Gly?Tyr?Leu?Asn?Ala?Asn1280????????????????1285????????????????1290????????????????1295GTG?CGC?TAC?AAC?AAC?GTG?GGC?ACC?GGC?GCC?ATC?TAC?GAC?GTG?AAG?CCC???????2591Val?Arg?Tyr?Asn?Asn?Val?Gly?Thr?Gly?Ala?Ile?Tyr?Asp?Val?Lys?Pro

1300????????????????1305????????????????1310ACC?ACC?AGC?TTC?GTG?CTG?AAC?AAC?GAC?ACC?ATC?GCC?ACC?ATC?ACC?GCC???????2639Thr?Thr?Ser?Phe?Val?Leu?Asn?Asn?Asp?Thr?Ile?Ala?Thr?Ile?Thr?Ala

1315????????????????1320????????????????1325AAG?TCG?AAT?TCC?ACC?GCC?CTG?AAC?ATC?AGC?CCC?GGC?GAG?AGC?TAC?CCC???????2687Lys?Ser?Asn?Ser?Thr?Ala?Leu?Asn?Ile?Ser?Pro?Gly?Glu?Ser?Tyr?Pro

1330????????????????1335????????????????1340AAG?AAG?GGC?CAG?AAC?GGC?ATC?GCC?ATC?ACC?AGC?ATG?GAC?GAC?TIC?AAC???????2735Lys?Lys?Gly?Gln?Asn?Gly?Ile?Ala?Ile?Thr?Ser?Met?Asp?Asp?Phe?Asn

1345????????????????1350????????????????1355AGC?CAC?CCC?ATC?ACC?CTG?AAC?AAG?AAG?CAG?GTG?GAC?AAC?CTG?CTG?AAC???????2783Ser?His?Pro?Ile?Thr?Leu?Asn?Lys?Lys?Gln?Val?Asp?Asn?Leu?Leu?Asn1360????????????????1365????????????????1370????????????????1375AAC?AAG?CCC?ATG?ATG?CTG?GAG?ACC?AAC?CAG?ACC?GAC?GGC?GTC?TAC?AAG???????2831Asn?Lys?Pro?Met?Met?Leu?Glu?Thr?Asn?Gln?Thr?Asp?Gly?Val?Tyr?Lys

1380????????????????1385????????????????1390ATC?AAG?GAC?ACC?CAC?GGC?AAC?ATC?GTG?ACG?GGC?GGC?GAG?TGG?AAC?GGC???????2879Ile?Lys?Asp?Thr?His?Gly?Asn?Ile?Val?Thr?Gly?Gly?Glu?Trp?Asn?Gly

1395????????????????1400????????????????1405GTG?ATC?CAG?CAG?ATC?AAG?GCC?AAG?ACC?GCC?AGC?ATC?ATC?GTC?GAC?GAC???????2927Val?Ile?Gln?Gln?Ile?Lys?Ala?Lys?Thr?Ala?Ser?Ile?Ile?Val?Asp?Asp

1410????????????????1415????????????????1420GGC?GAG?CGC?GTG?GCC?GAG?AAG?CGC?GTG?GCC?GCC?AAG?GAC?TAC?GAG?AAC???????2975Gly?Glu?Arg?Val?Ala?Glu?Lys?Arg?Val?Ala?Ala?Lys?Asp?Tyr?Glu?Asn

1425????????????????1430????????????????1435CCC?GAG?GAC?AAG?ACC?CCC?AGC?CTG?ACC?CTG?AAG?GAC?GCC?CTG?AAG?CTG???????3023Pro?Glu?Asp?Lys?Thr?Pro?Ser?Leu?Thr?Leu?Lys?Asp?Ala?Leu?Lys?Leu1440????????????????1445????????????????1450????????????????1455AGC?TAC?CCC?GAC?GAG?ATC?AAG?GAG?ATC?GAG?GGC?TTG?CTG?TAC?TAC?AAG???????3071Ser?Tyr?Pro?Asp?Glu?Ile?Lys?Glu?Ile?Glu?Gly?Leu?Leu?Tyr?Tyr?Lys

1460????????????????1465????????????????1470AAC?AAG?CCC?ATC?TAC?GAG?AGC?ACC?GTG?ATG?ACC?TAT?CTA?GAC?GAG?AAC???????3119Asn?Lys?Pro?Ile?Tyr?Glu?Ser?Ser?Val?Met?Thr?Tyr?Leu?Asp?Glu?Asn

1475????????????????1480????????????????1485ACC?GCC?AAG?GAG?GTG?ACC?AAG?CAG?CTG?AAC?GAC?ACC?ACC?GGC?AAG?TTC???????3167Thr?Ala?Lys?Glu?Val?Thr?Lys?Gln?Leu?Asn?Asp?Thr?Thr?Gly?Lys?Phe

1490????????????????1495????????????????1500AAG?GAC?GTG?AGC?CAC?CTG?TAC?GAC?GTG?AAG?CTG?ACC?CCC?AAG?ATG?AAC???????3215Lys?Asp?Val?Ser?His?Leu?Tyr?Asp?Val?Lys?Leu?Thr?Pro?Lys?Met?Asn

1505????????????????1510????????????????1515GTG?ACC?ATC?AAG?CTG?AGC?ATC?CTG?TAC?GAC?AAC?GCC?GAG?ACC?AAC?GAC???????3263Val?Thr?Ile?Lys?Leu?Ser?Ile?Leu?Tyr?Asp?Asn?Ala?Glu?Ser?Asn?Asp1520????????????????1525????????????????1530???????????????1535AAC?ACC?ATC?GGC?AAG?TGG?ACC?AAC?ACC?AAC?ATC?GTG?ACC?GGC?GGC?AAC???????3311Asn?Ser?Ile?Gly?Lys?Trp?Thr?Asn?Thr?Asn?Ile?Val?Ser?Gly?Gly?Asn

1540????????????????1545????????????????1550AAC?GGC?AAG?AAG?CAG?TAC?AGC?AGC?AAC?AAC?CCC?GAC?GCC?AAC?CTG?ACC???????3359Asn?Gly?Lys?Lys?Gln?Tyr?Ser?Ser?Asn?Asn?Pro?Asp?Ala?Asn?Leu?Thr

1555????????????????1560????????????????1565CTG?AAC?ACC?GAC?GCC?CAG?GAG?AAG?CTG?AAC?AAG?AAC?CGC?GAC?TAC?TAC???????3407Leu?Asn?Thr?Asp?Ala?Gln?Glu?Lys?Leu?Asn?Lys?Asn?Arg?Asp?Tyr?Tyr

1570????????????????1575????????????????1580ATC?AGC?CTG?TAC?ATG?AAG?AGC?GAG?AAG?AAC?ACC?CAG?TGC?GAG?ATC?ACC???????3455Ile?Ser?Leu?Tyr?Met?Lys?Ser?Glu?Lys?Asn?Thr?Gln?Cys?Glu?Ile?Thr

1585????????????????1590????????????????1595ATC?GAC?GGC?GAG?ATA?TAC?CCC?ATC?ACC?ACC?AAG?ACC?GTG?AAC?GTG?AAC????????3503Ile?Asp?Gly?Glu?Ile?Tyr?Pro?Ile?Thr?Thr?Lys?Thr?Val?Asn?Val?Asn1600????????????????1605????????????????1610????????????????1615AAG?GAC?AAC?TAC?AAG?CGC?CTG?GAC?ATC?ATC?GCC?CAC?AAC?ATC?AAG?AGC????????3551Lys?Asp?Asn?Tyr?Lys?Arg?Leu?Asp?Ile?Ile?Ala?His?Asn?Ile?Lys?Ser

1620????????????????1625????????????????1630AAC?CCC?ATC?AGC?AGC?CTG?CAC?ATC?AAG?ACC?AAC?GAC?GAG?ATC?ACC?CTG????????3599Asn?Pro?Ile?Ser?Ser?Leu?His?Ile?Lys?Thr?Asn?Asp?Glu?Ile?Thr?Leu

1635????????????????1640????????????????1645TTC?TGG?GAC?GAC?ATA?TCG?ATT?ACC?GAC?GTC?GCC?AGC?ATC?AAG?CCC?GAG????????3647Phe?Trp?Asp?Asp?Ile?Ser?Ile?Thr?Asp?Val?Ala?Ser?Ile?Lys?Pro?Glu

1650????????????????1655????????????????1660AAC?CTG?ACC?GAC?AGC?GAG?ATC?AAG?CAG?ATA?TAC?AGT?CGC?TAC?GGC?ATC????????3695Asn?Leu?Thr?Asp?Ser?Glu?Ile?Lys?Gln?Ile?Tyr?Ser?Arg?Tyr?Gly?Ile

1665????????????????1670????????????????1675AAG?CTG?GAG?GAC?GGC?ATC?CTG?ATC?GAC?AAG?AAA?GGC?GGC?ATC?CAC?TAC????????3743Lys?Leu?Glu?Asp?Gly?Ile?Leu?Ile?Asp?Lys?Lys?Gly?Gly?Ile?His?Tyr1680????????????????1685????????????????1690????????????????1695GGC?GAG?TTC?ATC?AAC?GAG?GCC?AGC?TIC?AAC?ATC?GAG?CCC?CTG?CAG?AAC????????3791Gly?Glu?Phe?Ile?Asn?Glu?Ala?Ser?Phe?Asn?Ile?Glu?Pro?Leu?Gln?Asn

1700????????????????1705????????????????1710TAC?GTG?ACC?AAG?TAC?GAG?GTG?ACC?TAC?AGC?AGC?GAG?CTG?GGC?CCC?AAC????????3839Tyr?Val?Thr?Lys?Tyr?Glu?Val?Thr?Tyr?Ser?Ser?Glu?Leu?Gly?Pro?Asn

1715????????????????1720????????????????1725GTG?AGC?GAC?ACC?eTG?GAG?AGC?GAC?AAG?ATT?TAC?AAG?GAC?GGC?ACC?ATC????????3887Val?Ser?Asp?Thr?Leu?Glu?Ser?Asp?Lys?Ile?Tyr?Lys?Asp?Gly?Thr?Ile

1730????????????????1735????????????????1740AAG?TTC?GAC?TTC?ACC?AAG?TAC?AGC?AAG?AAC?GAG?CAG?GGC?CTG?TTC?TAC????????3935Lys?Phe?Asp?Phe?Thr?Lys?Tyr?Ser?Lys?Asn?Glu?Gln?Gly?Leu?Phe?Tyr

1745????????????????1750????????????????1755GAC?AGC?GGC?CTG?AAC?TGG?GAC?TTC?AAG?ATC?AAC?GCC?ATC?ACC?TAC?GAC????????3983Asp?Ser?Gly?Leu?Asn?Trp?Asp?Phe?Lys?Ile?Asn?Ala?Ile?Thr?Tyr?Asp1760????????????????1765????????????????1770????????????????1775GGC?AAG?GAG?ATG?AAC?GTG?TTC?CAC?CGC?TAC?AAC?AAG?TAGATCTGAG?????????????4029Gly?Lys?Glu?Met?Asn?Val?Phe?His?Arg?Tyr?Asn?Lys

1780????????????????1785CT?????????????????????????????????????????????????????????????????????4031

(2) about the information of SEQ ID NO:50

(ⅰ) sequence signature

(A) length: 1338 amino acid

(B) type: amino acid

(D) topological structure: line style

(ⅱ) molecule type: protein

(ⅹ ⅰ) sequence description: SEQ ID NO:50:Met Lys Arg Met Glu Gly Lys Leu Phe Met Val Ser Lys Lys Leu Gln 15 10 15Val Val Thr Lys Thr Val Leu Leu Ser Thr Val Phe Ser Ile Ser Leu

20??????????????????25??????????????????30Leu?Asn?Asn?Glu?Val?Ile?Lys?Ala?Glu?Gln?Leu?Asn?Ile?Asn?Ser?Gln

35??????????????????40??????????????????45Ser?Lys?Tyr?Thr?Asn?Leu?Gln?Asn?Leu?Lys?Ile?Thr?Asp?Lys?Val?Glu

50??????????????????55??????????????????60Asp?Phe?Lys?Glu?Asp?Lys?Glu?Lys?Ala?Lys?Glu?Trp?Gly?Lys?Glu?Lys?65??????????????????70??????????????????75??????????????????80Glu?Lys?Glu?Trp?Lys?Leu?Thr?Ala?Thr?Glu?Lys?Gly?Lys?Met?Asn?Asn

85??????????????????90??????????????????95Phe?Leu?Asp?Asn?Lys?Asn?Asp?Ile?Lys?Thr?Asn?Tyr?Lys?Glu?Ile?Thr

100?????????????????105?????????????????110Phe?Ser?Ile?Ala?Gly?Ser?Phe?Glu?Asp?Glu?Ile?Lys?Asp?Leu?Lys?Glu

115?????????????????120?????????????????125Ile?Asp?Lys?Met?Phe?Asp?Lys?Thr?Asn?Leu?Ser?Asn?Ser?Ile?Ile?Thr

130?????????????????135?????????????????140Tyr?Lys?Asn?Val?Glu?Pro?Thr?Thr?Ile?Gly?Phe?Asn?Lys?Ser?Leu?Thr145????????????????150??????????????????155?????????????????160Glu?Gly?Asn?Thr?Ile?Asn?Ser?Asp?Ala?Met?Ala?Gln?Phe?Lys?Glu?Gln

165?????????????????170?????????????????175Phe?Leu?Asp?Arg?Asp?Ile?Lys?Phe?Asp?Ser?Tyr?Leu?Asp?Thr?His?Leu

180?????????????????185?????????????????190Thr?Ala?Gln?Gln?Val?Ser?Ser?Lys?Glu?Arg?Val?Ile?Leu?Lys?Val?Thr

195?????????????????200?????????????????205Val?Pro?Ser?Gly?Lys?Gly?Ser?Thr?Thr?Pro?Thr?Lys?Ala?Gly?Val?Ile

210?????????????????215?????????????????220Leu?Asn?Asn?Ser?Glu?Tyr?Lys?Met?Leu?Ile?Asp?Asn?Gly?Tyr?Met?Val225?????????????????230?????????????????235?????????????????240His?Val?Asp?Lys?Val?Ser?Lys?Val?Val?Lys?Lys?Gly?Val?Glu?Cys?Leu

245?????????????????250?????????????????255Gln?Ile?Glu?Gly?Thr?Leu?Lys?Lys?Ser?Leu?Asp?Phe?Lys?Asn?Asp?Ile

260?????????????????265?????????????????270Asn?Ala?Glu?Ala?His?Ser?Trp?Gly?Met?Lys?Asn?Tyr?Glu?Glu?Trp?Ala

275?????????????????280?????????????????285Lys?Asp?Leu?Thr?Asp?Ser?Gln?Arg?Glu?Ala?Leu?Asp?Gly?Tyr?Ala?Arg

290?????????????????295?????????????????300Gln?Asp?Tyr?Lys?Glu?Ile?Asn?Asn?Tyr?Leu?Arg?Asn?Gln?Gly?Gly?Ser305?????????????????310?????????????????315?????????????????320Gly?Asn?Glu?Lys?Leu?Asp?Ala?Gln?Ile?Lys?Asn?Ile?Ser?Asp?Ala?Leu

325?????????????????330??????????????????335Gly?Lys?Lys?Pro?Ile?Pro?Glu?Asn?Ile?Thr?Val?Tyr?Arg?Trp?Cys?Gly

340?????????????????345?????????????????350Met?Pro?Glu?Phe?Gly?Tyr?Gln?Ile?Ser?Asp?Pro?Leu?Pro?Ser?Leu?Lys

355?????????????????360?????????????????365Asp?Phe?Glu?Glu?Gln?Phe?Leu?Asn?Thr?Ile?Lys?Glu?Asp?Lys?Gly?Tyr

370?????????????????375?????????????????380Met?Ser?Thr?Ser?Leu?Ser?Ser?Glu?Arg?Leu?Ala?Ala?Phe?Gly?Ser?Arg385?????????????????390?????????????????395?????????????????400Lys?Ile?Ile?Leu?Arg?Leu?Gln?Val?Pro?Lys?Gly?Ser?Thr?Gly?Ala?Tyr

405?????????????????410?????????????????415Leu?Ser?Ala?Ile?Gly?Gly?Phe?Ala?Ser?Glu?Lys?Glu?Ile?Leu?Leu?Asp

420?????????????????425?????????????????430Lys?Asp?Ser?Lys?Tyr?His?Ile?Asp?Lys?Val?Thr?Glu?Val?Ile?Ile?Lys

435?????????????????440?????????????????445Gly?Val?Lys?Arg?Tyr?Val?Val?Asp?Ala?Thr?Leu?Leu?Thr?Asn?Ser?Arg

450?????????????????455?????????????????460Gly?Pro?Ser?Thr?Pro?Pro?Thr?Pro?Ser?Pro?Ser?Thr?Pro?Pro?Thr?Pro465?????????????????470?????????????????475?????????????????480Ser?Asp?Ile?Gly?Ser?Thr?Met?Lys?Thr?Asn?Gln?Ile?Ser?Thr?Thr?Gln

485?????????????????490?????????????????495Lys?Asn?Gln?Gln?Lys?Glu?Met?Asp?Arg?Lys?Gly?Leu?Leu?Gly?Tyr?Tyr

500?????????????????505?????????????????510Phe?Lys?Gly?Lys?Asp?Phe?Ser?Asn?Leu?Thr?Met?Phe?Ala?Pro?Thr?Arg

515?????????????????520?????????????????525Asp?Ser?Thr?Leu?Ile?Tyr?Asp?Gln?Gln?Thr?Ala?Asn?Lys?Leu?Leu?Asp

530?????????????????535?????????????????540Lys?Lys?Gln?Gln?Glu?Tyr?Gln?Ser?Ile?Arg?Trp?Ile?Gly?Leu?Ile?Gln545?????????????????550?????????????????555?????????????????560Ser?Lys?Glu?Thr?Gly?Asp?Phe?Thr?Phe?Asn?Leu?Ser?Glu?Asp?Glu?Gln

565?????????????????570?????????????????575Ala?Ile?Ile?Glu?Ile?Asn?Gly?Lys?Ile?Ile?Ser?Asn?Lys?Gly?Lys?Glu

580??????????????????585?????????????????590Lys?Gln?Val?Val?His?Leu?Glu?Lys?Gly?Lys?Leu?Val?Pro?Ile?Lys?Ile

595?????????????????600?????????????????605Glu?Tyr?Gln?Ser?Asp?Thr?Lys?Phe?Asn?Ile?Asp?Ser?Lys?Thr?Phe?Lys

610?????????????????615?????????????????620Glu?Leu?Lys?Leu?Phe?Lys?Ile?Asp?Ser?Gln?Asn?Gln?Pro?Gln?Gln?Val625?????????????????630?????????????????635?????????????????640Gln?Gln?Asp?Glu?Leu?Arg?Asn?Pro?Glu?Phe?Asn?Lys?Lys?Glu?Ser?Gln

645?????????????????650?????????????????655Glu?Phe?Leu?Ala?Lys?Pro?Ser?Lys?Ile?Asn?Leu?Phe?Thr?Gln?Gln?Met

660?????????????????665?????????????????670Lys?Arg?Glu?Ile?Asp?Glu?Asp?Thr?Asp?Thr?Asp?Gly?Asp?Ser?Ile?Pro

675?????????????????680?????????????????685Asp?Leu?Trp?Glu?Glu?Asn?Gly?Tyr?Thr?Ile?Gln?Asn?Arg?Ile?Ala?Val

690?????????????????695?????????????????700Lys?Trp?Asp?Asp?Ser?Leu?Ala?Ser?Lys?Gly?Tyr?Thr?Lys?Phe?Val?Ser705?????????????????710?????????????????715?????????????????720Asn?Pro?Leu?Glu?Ser?His?Thr?Val?Gly?Asp?Pro?Tyr?Thr?Asp?Tyr?Glu

725?????????????????730?????????????????735Lys?Ala?Ala?Arg?Asp?Leu?Asp?Leu?Ser?Asn?Ala?Lys?Glu?Thr?Phe?Asn

740?????????????????745????????????????750Pro?Leu?Val?Ala?Ala?Phe?Pro?Ser?Val?Asn?Val?Ser?Met?Glu?Lys?Val

755?????????????????760?????????????????765Ile?Leu?Ser?Pro?Asn?Glu?Asn?Leu?Ser?Asn?Ser?Val?Glu?Ser?His?Ser

770?????????????????775?????????????????780Ser?Thr?Asn?Trp?Ser?Tyr?Thr?Asn?Thr?Glu?Gly?Ala?Ser?Val?Glu?Ala785?????????????????790?????????????????795?????????????????800Gly?Ile?Gly?Pro?Lys?Gly?Ile?Ser?Phe?Gly?Val?Ser?Val?Asn?Tyr?Gln

805?????????????????810?????????????????815His?Ser?Glu?Thr?Val?Ala?Gln?Glu?Trp?Gly?Thr?Ser?Thr?Gly?Asn?Thr

820?????????????????825?????????????????830Ser?Gln?Phe?Asn?Thr?Ala?Ser?Ala?Gly?Tyr?Leu?Asn?Ala?Asn?Val?Arg

835?????????????????840?????????????????845Tyr?Asn?Asn?Val?Gly?Thr?Gly?Ala?Ile?Tyr?Asp?Val?Lys?Pro?Thr?Thr

850??????????????????855????????????????860Ser?Phe?Val?Leu?Asn?Asn?Asp?Thr?Ile?Ala?Thr?Ile?Thr?Ala?Lys?Ser865?????????????????870?????????????????875?????????????????880Asn?Ser?Thr?Ala?Leu?Asn?Ile?Ser?Pro?Gly?Glu?Ser?Tyr?Pro?Lys?Lys

885?????????????????890?????????????????895Gly?Gln?Asn?Gly?Ile?Ala?Ile?Thr?Ser?Met?Asp?Asp?Phe?Asn?Ser?His

900?????????????????905?????????????????910Pro?Ile?Thr?Leu?Asn?Lys?Lys?Gln?Val?Asp?Asn?Leu?Leu?Asn?Asn?Lys

915??????????????????920????????????????925Pro?Met?Met?Leu?Glu?Thr?Asn?Gln?Thr?Asp?Gly?Val?Tyr?Lys?Ile?Lys

930?????????????????935?????????????????940Asp?Thr?His?Gly?Asn?Ile?Val?Thr?Gly?Gly?Glu?Trp?Asn?Gly?Val?Ile945?????????????????950?????????????????955?????????????????960Gln?Gln?Ile?Lys?Ala?Lys?Thr?Ala?Ser?Ile?Ile?Val?Asp?Asp?Gly?Glu

965?????????????????970?????????????????975Arg?Val?Ala?Glu?Lys?Arg?Val?Ala?Ala?Lys?Asp?Tyr?Glu?Asn?Pro?Glu

980?????????????????985?????????????????990Asp?Lys?Thr?Pro?Ser?Leu?Thr?Leu?Lys?Asp?Ala?Leu?Lys?Leu?Ser?Tyr

995?????????????????1000????????????????1005Pro?Asp?Glu?Ile?Lys?Glu?Ile?Glu?Gly?Leu?Leu?Tyr?Tyr?Lys?Asn?Lys

1010????????????????1015????????????????1020Pro?Ile?Tyr?Glu?Ser?Ser?Val?Met?Thr?Tyr?Leu?Asp?Glu?Asn?Thr?Ala1025????????????????1030????????????????1035????????????????1040Lys?Glu?Val?Thr?Lys?Gln?Leu?Asn?Asp?Thr?Thr?Gly?Lys?Phe?Lys?Asp

1045????????????????1050????????????????1055Val?Ser?His?Leu?Tyr?Asp?Val?Lys?Leu?Thr?Pro?Lys?Met?Asn?Val?Thr

1060????????????????1065????????????????1070Ile?Lys?Leu?Ser?Ile?Leu?Tyr?Asp?Asn?Ala?Glu?Ser?Asn?Asp?Asn?Ser

1075????????????????1080????????????????1085Ile?Gly?Lys?Trp?Thr?Asn?Thr?Asn?Ile?Val?Ser?Gly?Gly?Asn?Asn?Gly

1090????????????????1095????????????????1100Lys?Lys?Gln?Tyr?Ser?Ser?Asn?Asn?Pro?Asp?Ala?Asn?Leu?Thr?Leu?Asn1105????????????????1110????????????????1115????????????????1120Thr?Asp?Ala?Gln?Glu?Lys?Leu?Asn?Lys?Asn?Arg?Asp?Tyr?Tyr?Ile?Ser

1125????????????????1130????????????????1135Leu?Tyr?Met?Lys?Ser?Glu?Lys?Asn?Thr?Gln?Cys?Glu?Ile?Thr?Ile?Asp

1140????????????????1145????????????????1150Gly?Glu?Ile?Tyr?Pro?Ile?Thr?Thr?Lys?Thr?Val?Asn?Val?Asn?Lys?Asp

1155????????????????1160????????????????1165Asn?Tyr?Lys?Arg?Leu?Asp?Ile?Ile?Ala?His?Asn?Ile?Lys?Ser?Asn?Pro

1170????????????????1175????????????????1180Ile?Ser?Ser?Leu?His?Ile?Lys?Thr?Asn?Asp?Glu?Ile?Thr?Leu?Phe?Trp1185????????????????1190????????????????1195????????????????1200Asp?Asp?Ile?Ser?Ile?Thr?Asp?Val?Ala?Ser?Ile?Lys?Pro?Glu?Asn?Leu

1205????????????????1210????????????????1215Thr?Asp?Ser?Glu?Ile?Lys?Gln?Ile?Tyr?Ser?Arg?Tyr?Gly?Ile?Lys?Leu

1220????????????????1225????????????????1230Glu?Asp?Gly?Ile?Leu?Ile?Asp?Lys?Lys?Gly?Gly?Ile?His?Tyr?Gly?Glu

1235????????????????1240????????????????1245Phe?Ile?Asn?Glu?Ala?Ser?Phe?Asn?Ile?Glu?Pro?Leu?Gln?Asn?Tyr?Val

1250????????????????1255????????????????1260Thr?Lys?Tyr?Glu?Val?Thr?Tyr?Ser?Ser?Glu?Leu?Gly?Pro?Asn?Val?Ser1265????????????????1270????????????????1275????????????????1280Asp?Thr?Leu?Glu?Ser?Asp?Lys?Ile?Tyr?Lys?Asp?Gly?Thr?Ile?Lys?Phe

1285????????????????1290????????????????1295Asp?Phe?Thr?Lys?Tyr?Ser?Lys?Asn?Glu?Gln?Gly?Leu?Phe?Tyr?Asp?Ser

1300?????????????????1305????????????????1310Gly?Leu?Asn?Trp?Asp?Phe?Lys?Ile?Asn?Ala?Ile?Thr?Tyr?Asp?Gly?Lys

1315????????????????1320????????????????1325Glu?Met?Asn?Val?Phe?His?Arg?Tyr?Asn?Lys

1330????????????????1335

(2) about the information of SEQ ID NO:51

(ⅰ) sequence signature

(A) length: 2444 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: DNA (genome)

(ⅲ) the false plan: not

(ⅸ) feature:

(A) title/key: CDS

(B) position: 17..2444

(D) out of Memory :/product=" 3A (a) is synthetic: natural fusion "

(ⅹ ⅰ) sequence description: SEQ ID NO:51:GGATCCACCA ATGAAC ATG AAC AAG AAC AAC ACC AAG CTG AGC ACC CGC 49

Met?Asn?Lys?Asn?Asn?Thr?Lys?Leu?Ser?Thr?Arg

1???????????????5??????????????????10GCC?CTG?CCG?AGC?TTC?ATC?GAC?TAC?TTC?AAC?GGC?ATC?TAC?GGC?TTC?GCC?????????97Ala?Leu?Pro?Ser?Phe?Ile?Asp?Tyr?Phe?Asn?Gly?Ile?Tyr?Gly?Phe?Ala

15??????????????????20??????????????????25ACC?GGC?ATC?AAG?GAC?ATC?ATG?AAC?ATG?ATC?TTC?AAG?ACC?GAC?ACC?GGC????????145Thr?Gly?Ile?Lys?Asp?Ile?Met?Asn?Met?Ile?Phe?Lys?Thr?Asp?Thr?Gly

30??????????????????35??????????????????40GGC?GAC?CTG?ACC?CTG?GAC?GAG?ATC?CTG?AAG?AAC?CAG?CAG?CTG?CTG?AAC????????193Gly?Asp?Leu?Thr?Leu?Asp?Glu?Ile?Leu?Lys?Asn?Gln?Gln?Leu?Leu?Asn

45??????????????????50??????????????????55GAC?ATC?AGC?GGC?AAG?CTG?GAC?GGC?GTG?AAC?GGC?AGC?CTG?AAC?GAC?CTG????????241Asp?Ile?Ser?Gly?Lys?Leu?Asp?Gly?Val?Asn?Gly?Ser?Leu?Asn?Asp?Leu?60??????????????????65??????????????????70??????????????????75ATC?GCC?CAG?GGC?AAC?CTG?AAC?ACC?GAG?CTG?AGC?AAG?GAG?ATC?CTT?AAG????????289Ile?Ala?Gln?Gly?Asn?Leu?Asn?Thr?Glu?Leu?Ser?Lys?Glu?Ile?Leu?Lys

80??????????????????85??????????????????90ATC?GCC?AAC?GAG?CAG?AAC?CAG?GTG?CTG?AAC?GAC?GTG?AAC?AAC?AAG?CTG????????337Ile?Ala?Asn?Glu?Gln?Asn?Gln?Val?Leu?Asn?Asp?Val?Asn?Asn?Lys?Leu

95?????????????????100?????????????????105GAC?GCC?ATC?AAC?ACC?ATG?CTG?CGC?GTG?TAC?CTG?CCG?AAG?ATC?ACC?AGC????????385Asp?Ala?Ile?Asn?Thr?Met?Leu?Arg?Val?Tyr?Leu?Pro?Lys?Ile?Thr?Ser

110?????????????????115?????????????????120ATG?CTG?AGC?GAC?GTG?ATG?AAG?CAG?AAC?TAC?GCC?CTG?AGC?CTG?CAG?ATC????????433Met?Leu?Ser?Asp?Val?Met?Lys?Gln?Asn?Tyr?Ala?Leu?Ser?Leu?Gln?Ile

125?????????????????130?????????????????135GAG?TAC?CTG?AGC?AAG?CAG?CTG?CAG?GAG?ATC?AGC?GAC?AAG?CTG?GAC?ATC????????481Glu?Tyr?Leu?Ser?Lys?Gln?Leu?Gln?Glu?Ile?Ser?Asp?Lys?Leu?Asp?Ile140?????????????????145?????????????????150?????????????????155ATC?AAC?GTG?AAC?GTC?CTG?ATC?AAC?AGC?ACC?CTG?ACC?GAG?ATC?ACC?CCG????????529Ile?Asn?Val?Asn?Val?Leu?Ile?Asn?Ser?Thr?Leu?Thr?Glu?Ile?Thr?Pro

160?????????????????165?????????????????170GCC?TAC?CAG?CGC?ATC?AAG?TAC?GTG?AAC?GAG?AAG?TTC?GAA?GAG?CTG?ACC????????577Ala?Tyr?Gln?Arg?Ile?Lys?Tyr?Val?Asn?Glu?Lys?Phe?Glu?Glu?Leu?Thr

175?????????????????180?????????????????185TTC?GCC?ACC?GAG?ACC?AGC?AGC?AAG?GTG?AAG?AAG?GAC?GGC?AGC?CCG?GCC????????625Phe?Ala?Thr?Glu?Thr?Ser?Ser?Lys?Val?Lys?Lys?Asp?Gly?Ser?Pro?Ala

190?????????????????195?????????????????200GAC?ATC?CTG?GAC?GAG?CTG?ACC?GAG?CTG?ACC?GAG?CTG?GCC?AAG?AGC?GTG????????673Asp?Ile?Leu?Asp?Glu?Leu?Thr?Glu?Leu?Thr?Glu?Leu?Ala?Lys?Ser?Val

205?????????????????210?????????????????215ACC?AAG?AAC?GAC?GTG?GAC?GGC?TTC?GAG?TTC?TAC?CTG?AAC?ACC?TTC?CAC????????721Thr?Lys?Asn?Asp?Val?Asp?Gly?Phe?Glu?Phe?Tyr?Leu?Asn?Thr?Phe?His220?????????????????225?????????????????230?????????????????235GAC?GTG?ATG?GTG?GGC?AAC?AAC?CTG?TTC?GGC?CGC?AGC?GCC?CTG?AAG?ACC????????769Asp?Val?Met?Val?Gly?Asn?Asn?Leu?Phe?Gly?Arg?Ser?Ala?Leu?Lys?Thr

240?????????????????245?????????????????250GCC?AGC?GAG?CTG?ATC?ACC?AAG?GAG?AAC?GTG?AAG?ACC?AGC?GGC?AGC?GAG????????817Ala?Ser?Glu?Leu?Ile?Thr?Lys?Glu?Asn?Val?Lys?Thr?Ser?Gly?Ser?Glu

255?????????????????260?????????????????265GTG?GGC?AAC?GTG?TAC?AAC?TTC?CTG?ATC?GTG?CTG?ACC?GCC?CTG?CAG?GCC????????865Val?Gly?Asn?Val?Tyr?Asn?Phe?Leu?Ile?Val?Leu?Thr?Ala?Leu?Gln?Ala

270?????????????????275?????????????????280CAG?GCC?TTC?CTG?ACC?CTG?ACC?ACC?TGT?CGC?AAG?CTG?CTG?GGC?CTG?GCC????????913Gln?Ala?Phe?Leu?Thr?Leu?Thr?Thr?Cys?Arg?Lys?Leu?Leu?Gly?Leu?Ala

285?????????????????290?????????????????295GAC?ATC?GAC?TAC?ACC?AGC?ATC?ATG?AAC?GAG?CAC?TTG?AAC?AAG?GAG?AAG????????961Asp?Ile?Asp?Tyr?Thr?Ser?Ile?Met?Asn?Glu?His?Leu?Asn?Lys?Glu?Lys300?????????????????305?????????????????310?????????????????315GAG?GAG?TTC?CGC?GTG?AAC?ATC?CTG?CCG?ACC?CTG?AGC?AAC?ACC?TTC?AGC???????1009Glu?Glu?Phe?Arg?Val?Asn?Ile?Leu?Pro?Thr?Leu?Ser?Asn?Thr?Phe?Ser

320?????????????????325?????????????????330AAC?CCG?AAC?TAC?GCC?AAG?GTG?AAG?GGC?AGC?GAC?GAG?GAC?GCC?AAG?ATG???????1057Asn?Pro?Asn?Tyr?Ala?Lys?Val?Lys?Gly?Ser?Asp?Glu?Asp?Ala?Lys?Met

335?????????????????340?????????????????345ATC?GTG?GAG?GCT?AAG?CCG?GGC?CAC?GCG?TTG?ATC?GGC?TTC?GAG?ATC?AGC???????1105Ile?Val?Glu?Ala?Lys?Pro?Gly?His?Ala?Leu?Ile?Gly?Phe?Glu?Ile?Ser

350?????????????????355?????????????????360AAC?GAC?AGC?ATC?ACC?GTG?CrG?AAG?GTG?TAC?GAG?GCC?AAG?CTG?AAG?CAG???????1153Asn?Asp?Ser?Ile?Thr?Val?Leu?Lys?Val?Tyr?Glu?Ala?Lys?Leu?Lys?Gln

365?????????????????370?????????????????375AAC?TAC?CAG?GTG?GAC?AAG?GAC?AGC?TTG?AGC?GAG?GTG?ATC?TAC?GGC?GAC???????1201Asn?Tyr?Gln?Val?Asp?Lys?Asp?Ser?Leu?Ser?Glu?Val?Ile?Tyr?Gly?Asp380?????????????????385?????????????????390?????????????????395ATG?GAC?AAG?CTG?CTG?TGT?CCG?GAC?CAG?AGC?GAG?CAA?ATC?TAC?TAC?ACC???????1249Met?Asp?Lys?Leu?Leu?Cys?Pro?Asp?Gln?Ser?Glu?Gln?Ile?Tyr?Tyr?Thr

400?????????????????405?????????????????410AAC?AAC?ATC?GTG?TTC?CCG?AAC?GAG?TAC?GTG?ATC?ACC?AAG?ATC?GAC?TTT????????1297Asn?Asn?Ile?Val?Phe?Pro?Asn?Glu?Tyr?Val?Ile?Thr?Lys?Ile?Asp?Phe

415?????????????????420?????????????????425ACC?AAG?AAG?ATG?AAG?ACC?CTG?CGC?TAC?GAG?GTG?ACC?GCC?AAC?TTC?TAC???????1345Thr?Lys?Lys?Met?Lys?Thr?Leu?Arg?Tyr?Glu?Val?Thr?Ala?Asn?Phe?Tyr

430?????????????????435?????????????????440GAC?AGC?AGC?ACC?GGC?GAG?ATC?GAC?CTG?AAC?AAG?AAG?AAG?GTG?GAG?AGC????????1393Asp?Ser?Ser?Thr?Gly?Glu?Ile?Asp?Leu?Asn?Lys?Lys?Lys?Val?Glu?Ser

445?????????????????450?????????????????455AGC?GAG?GCC?GAG?TAC?CGC?ACC?CTG?AGC?GCG?AAC?GAC?GAC?GGC?GTC?TAC????????1441Ser?Glu?Ala?Glu?Tyr?Arg?Thr?Leu?Ser?Ala?Asn?Asp?Asp?Gly?Val?Tyr460?????????????????465?????????????????470?????????????????475ATG?CCA?CTG?GGC?GTG?ATC?AGC?GAG?ACC?TTC?CTG?ACC?CCG?ATC?AAC?GGC????????1489Met?Pro?Leu?Gly?Val?Ile?Ser?Glu?Thr?Phe?Leu?Thr?Pro?Ile?Asn?Gly

480?????????????????485?????????????????490TTT?GGC?CTG?CAG?GCC?GAC?GAG?AAC?AGC?CGC?CTG?ATC?ACC?CTG?ACC?TGT????????1537Phe?Gly?Leu?Gln?Ala?Asp?Glu?Asn?Ser?Arg?Leu?Tle?Thr?Leu?Thr?Cys

495?????????????????500?????????????????505AAG?AGC?TAC?CTG?CGC?GAG?CTG?CTG?CTA?GCC?ACC?GAC?CTG?AGC?AAC?AAG????????1585Lys?Ser?Tyr?Leu?Arg?Glu?Leu?Leu?Leu?Ala?Thr?Asp?Leu?Ser?Asn?Lys

510?????????????????515?????????????????520GAG?ACC?AAG?CTG?ATC?GTG?CCA?CCG?AGC?GGC?TTC?ATC?AGC?AAC?ATC?GTG????????1633Glu?Thr?Lys?Leu?Ile?Val?Pro?Pro?Ser?Gly?Phe?Ile?Ser?Asn?Ile?Val

525?????????????????530?????????????????535GAG?AAC?GGC?AGC?ATC?GAG?GAG?GAC?AAC?CTG?GAG?CCG?TGG?AAG?GCC?AAC????????1681Glu?Asn?Gly?Ser?Ile?Glu?Glu?Asp?Asn?Leu?Glu?Pro?Trp?Lys?Ala?Asn540?????????????????545?????????????????550?????????????????555AAC?AAG?AAC?GCC?TAC?GTG?GAC?CAC?ACC?GGC?GGC?GTG?AAC?GGC?ACC?AAG????????1729Asn?Lys?Asn?Ala?Tyr?Val?Asp?His?Thr?Gly?Gly?Val?Asn?Gly?Thr?Lys

560?????????????????565?????????????????570GCC?CTG?TAC?GTG?CAC?AAG?GAC?GGC?GGC?ATC?AGC?CAG?TTC?ATC?GGC?GAC????????1777Ala?Leu?Tyr?Val?His?Lys?Asp?Gly?Gly?Ile?Ser?Gln?Phe?Ile?Gly?Asp

575?????????????????580?????????????????585AAG?CTG?AAG?CCG?AAG?ACC?GAG?TAC?GTG?ATC?CAG?TAC?ACC?GTG?AAG?GGC????????1825Lys?Leu?Lys?Pro?Lys?Thr?Glu?Tyr?Val?Ile?Gln?Tyr?Thr?Val?Lys?Gly

590?????????????????595?????????????????600AAG?CCA?TCG?ATT?CAC?CTG?AAG?GAC?GAG?AAC?ACC?GGC?TAC?ATC?CAC?TAC????????1873Lys?Pro?Ser?Ile?His?Leu?Lys?Asp?Glu?Asn?Thr?Gly?Tyr?Ile?His?Tyr

605?????????????????610?????????????????615GAG?GAC?ACC?AAC?AAC?AAC?CTG?GAG?GAC?TAC?CAG?ACC?ATC?AAC?AAG?CGC????????1921Glu?Asp?Thr?Asn?Asn?Asn?Leu?Glu?Asp?Tyr?Gln?Thr?Ile?Asn?Lys?Arg620?????????????????625?????????????????630?????????????????635TTC?ACC?ACC?GGC?ACC?GAC?CTG?AAG?GGC?GTG?TAC?CTG?ATC?CTG?AAG?AGC???????1969Phe?Thr?Thr?Gly?Thr?Asp?Leu?Lys?Gly?Val?Tyr?Leu?Ile?Leu?Lys?Ser

640?????????????????645?????????????????650CAG?AAC?GGC?GAC?GAG?GCC?TGG?GGC?GAC?AAC?TTC?ATC?ATC?CTG?GAG?ATC???????2017Gln?Asn?Gly?Asp?Glu?Ala?Trp?Gly?Asp?Asn?Phe?Ile?Ile?Leu?Glu?Ile

655?????????????????660?????????????????665AGC?CCG?AGC?GAG?AAG?CTG?CTG?AGC?CCG?GAG?CTG?ATC?AAC?ACC?AAC?AAC???????2065Ser?Pro?Ser?Glu?Lys?Leu?Leu?Ser?Pro?Glu?Leu?Ile?Asn?Thr?Asn?Asn

670?????????????????675?????????????????680TGG?ACC?AGC?ACC?GGC?AGC?ACC?AAC?ATC?AGC?GGC?AAC?ACC?CTG?ACC?CTG???????2113Trp?Thr?Ser?Thr?Gly?Ser?Thr?Asn?Ile?Ser?Gly?Asn?Thr?Leu?Thr?Leu

685?????????????????690?????????????????695TAC?CAG?GGC?GGC?CGG?GGG?ATT?CTA?AAA?CAA?AAC?CTT?CAA?TTA?GAT?AGT???????2161Tyr?Gln?Gly?Gly?Arg?Gly?Ile?Leu?Lys?Gln?Asn?Leu?Gln?Leu?Asp?Ser700?????????????????705?????????????????710?????????????????715TTT?TCA?ACT?TAT?AGA?GTG?TAT?TTT?TCT?GTG?TCC?GGA?GAT?GCT?AAT?GTA???????2209Phe?Ser?Thr?Tyr?Arg?Val?Tyr?Phe?Ser?Val?Ser?Gly?Asp?Ala?Asn?Val

720?????????????????725?????????????????730AGG?ATT?AGA?AAT?TCT?AGG?GAA?GTG?TTA?TTT?GAA?AAA?AGA?TAT?ATG?AGC???????2257Arg?Ile?Arg?Asn?Ser?Arg?Glu?Val?Leu?Phe?Glu?Lys?Arg?Tyr?Met?Ser

735?????????????????740?????????????????745GGT?GCT?AAA?GAT?GTT?TCT?GAA?ATG?TTC?ACT?ACA?AAA?TTT?GAG?AAA?GAT???????2305Gly?Ala?Lys?Asp?Val?Ser?Glu?Met?Phe?Thr?Thr?Lys?Phe?Glu?Lys?Asp

750?????????????????755?????????????????760AAC?TTT?TAT?ATA?GAG?CTT?TCT?CAA?GGG?AAT?AAT?TTA?TAT?GGT?GGT?CCT???????2353Asn?Phe?Tyr?Ile?Glu?Leu?Ser?Gln?Gly?Asn?Asn?Leu?Tyr?Gly?Gly?Pro

765?????????????????770?????????????????775ATT?GTA?CAT?TTT?TAC?GAT?GTC?TCT?ATT?AAG?NAA?GAT?CGG?GAT?CTA?ATA???????2401Ile?Val?His?Phe?Tyr?Asp?Val?Ser?Ile?Lys?Xaa?Asp?Arg?Asp?Leu?Ile780?????????????????785?????????????????790???????????????????795TTA?ACA?GTT?TTT?AAA?AGC?NAA?TTC?TTG?TAT?AAT?GTC?CTT?GAT???????????????2444Leu?Thr?Val?Phe?Lys?Ser?Xaa?Phe?Leu?Tyr?Asn?Val?Leu?Asp

800?????????????????805

(2) about the information of SEQ ID NO:52

(ⅰ) sequence signature

(A) length: 809 amino acid

(B) type: amino acid

(D) topological structure: line style

(ⅱ) molecule type: protein

(ⅹ ⅰ) sequence description: SEQ ID NO:52:Met Asn Lys Asn Asn Thr Lys Leu Ser Thr Arg Ala Leu Pro Ser Phe 15 10 15Ile Asp Tyr Phe Asn Gly Ile Tyr Gly Phe Ala Thr Gly Ile Lys Asp

20??????????????????25??????????????????30Ile?Met?Asn?Met?Ile?Phe?Lys?Thr?Asp?Thr?Gly?Gly?Asp?Leu?Thr?Leu

35??????????????????40??????????????????45Asp?Glu?Ile?Leu?Lys?Asn?Gln?Gln?Leu?Leu?Asn?Asp?Ile?Ser?Gly?Lys

50??????????????????55??????????????????60Leu?Asp?Gly?Val?Asn?Gly?Ser?Leu?Asn?Asp?Leu?Ile?Ala?Gln?Gly?Asn?65??????????????????70??????????????????75??????????????????80Leu?Asn?Thr?Glu?Leu?Ser?Lys?Glu?Ile?Leu?Lys?Ile?Ala?Asn?Glu?Gln

85??????????????????90??????????????????95Asn?Gln?Val?Leu?Asn?Asp?Val?Asn?Asn?Lys?Leu?Asp?Ala?Ile?Asn?Thr

100????????????????105??????????????????110Met?Leu?Arg?Val?Tyr?Leu?Pro?Lys?Ile?Thr?Ser?Met?Leu?Ser?Asp?Val

115?????????????????120?????????????????125Met?Lys?Gln?Asn?Tyr?Ala?Leu?Ser?Leu?Gln?Ile?Glu?Tyr?Leu?Ser?Lys

130?????????????????135?????????????????140Gln?Leu?Gln?Glu?Ile?Ser?Asp?Lys?Leu?Asp?Ile?Ile?Asn?Val?Asn?Val145?????????????????150?????????????????155?????????????????160Leu?Ile?Asn?Ser?Thr?Leu?Thr?Glu?Ile?Thr?Pro?Ala?Tyr?Gln?Arg?Ile

165?????????????????170?????????????????175Lys?Tyr?Val?Asn?Glu?Lys?Phe?Glu?Glu?Leu?Thr?Phe?Ala?Thr?Glu?Thr

180?????????????????185?????????????????190Ser?Ser?Lys?Val?Lys?Lys?Asp?Gly?Ser?Pro?Ala?Asp?Ile?Leu?Asp?Glu

195?????????????????200?????????????????205Leu?Thr?Glu?Leu?Thr?Glu?Leu?Ala?Lys?Ser?Val?Thr?Lys?Asn?Asp?Val

210?????????????????215?????????????????220Asp?Gly?Phe?Glu?Phe?Tyr?Leu?Asn?Thr?Phe?His?Asp?Val?Met?Val?Gly225?????????????????230?????????????????235?????????????????240Asn?Asn?Leu?Phe?Gly?Arg?Ser?Ala?Leu?Lys?Thr?Ala?Ser?Glu?Leu?Ile

245?????????????????250?????????????????255Thr?Lys?Glu?Asn?Val?Lys?Thr?Ser?Gly?Ser?Glu?Val?Gly?Asn?Val?Tyr

260?????????????????265?????????????????270Asn?Phe?Leu?Ile?Val?Leu?Thr?Ala?Leu?Gln?Ala?Gln?Ala?Phe?Leu?Thr

275?????????????????280?????????????????285Leu?Thr?Thr?Cys?Arg?Lys?Leu?Leu?Gly?Leu?Ala?Asp?Ile?Asp?Tyr?Thr

290?????????????????295?????????????????300Ser?Ile?Met?Asn?Glu?His?Leu?Asn?Lys?Glu?Lys?Glu?Glu?Phe?Arg?Val305?????????????????310?????????????????315?????????????????320Asn?Ile?Leu?Pro?Thr?Leu?Ser?Asn?Thr?Phe?Ser?Asn?Pro?Asn?Tyr?Ala

325?????????????????330?????????????????335Lys?Val?Lys?Gly?Ser?Asp?Glu?Asp?Ala?Lys?Met?Ile?Val?Glu?Ala?Lys

340??????????????????345?????????????????350Pro?Gly?His?Ala?Leu?Ile?Gly?Phe?Glu?Ile?Ser?Asn?Asp?Set?Ile?Thr

355?????????????????360?????????????????365Val?Leu?Lys?Val?Tyr?Glu?Ala?Lys?Leu?Lys?Gln?Asn?Tyr?Gln?Val?Asp

370?????????????????375?????????????????380Lys?Asp?Ser?Leu?Ser?Glu?Val?Ile?Tyr?Gly?Asp?Met?Asp?Lys?Leu?Leu385?????????????????390?????????????????395?????????????????400Cys?Pro?Asp?Gln?Ser?Glu?Gln?Ile?Tyr?Tyr?Thr?Asn?Asn?Ile?Val?Phe

405?????????????????410?????????????????415Pro?Asn?Glu?Tyr?Val?Ile?Thr?Lys?Ile?Asp?Phe?Thr?Lys?Lys?Met?Lys

420?????????????????425?????????????????430Thr?Leu?Arg?Tyr?Glu?Val?Thr?Ala?Asn?Phe?Tyr?Asp?Ser?Ser?Thr?Gly

435?????????????????440?????????????????445Glu?Ile?Asp?Leu?Asn?Lys?Lys?Lys?Val?Glu?Ser?Ser?Glu?Ala?Glu?Tyr

450?????????????????455?????????????????460Arg?Thr?Leu?Ser?Ala?Asn?Asp?Asp?Gly?Val?Tyr?Met?Pro?Leu?Gly?Val465?????????????????470?????????????????475?????????????????480Ile?Ser?Glu?Thr?Phe?Leu?Thr?Pro?Ile?Asn?Gly?Phe?Gly?Leu?Gln?Ala

485?????????????????490?????????????????495Asp?Glu?Asn?Ser?Arg?Leu?Ile?Thr?Leu?Thr?Cys?Lys?Ser?Tyr?Leu?Arg

500?????????????????505?????????????????510Glu?Leu?Leu?Leu?Ala?Thr?Asp?Leu?Ser?Asn?Lys?Glu?Thr?Lys?Leu?Ile

515?????????????????520?????????????????525Val?Pro?Pro?Ser?Gly?Phe?Ile?Ser?Asn?Ile?Val?Glu?Asn?Gly?Ser?Ile

530?????????????????535?????????????????540Glu?Glu?Asp?Asn?Leu?Glu?Pro?Trp?Lys?Ala?Asn?Asn?Lys?Asn?Ala?Tyr545?????????????????550?????????????????555?????????????????560Val?Asp?His?Thr?Gly?Gly?Val?Asn?Gly?Thr?Lys?Ala?Leu?Tyr?Val?His

565?????????????????570?????????????????575Lys?Asp?Gly?Gly?Ile?Ser?Gln?Phe?Ile?Gly?Asp?Lys?Leu?Lys?Pro?Lys

580?????????????????585??????????????????590Thr?Glu?Tyr?Val?Ile?Gln?Tyr?Thr?Val?Lys?Gly?Lys?Pro?Ser?Ile?His

595?????????????????600?????????????????605Leu?Lys?Asp?Glu?Asn?Thr?Gly?Tyr?Ile?His?Tyr?Glu?Asp?Thr?Asn?Asn

610?????????????????615?????????????????620Asn?Leu?Glu?Asp?Tyr?Gln?Thr?Ile?Asn?Lys?Arg?Phe?Thr?Thr?Gly?Thr625?????????????????630?????????????????635?????????????????640Asp?Leu?Lys?Gly?Val?Tyr?Leu?Ile?Leu?Lys?Ser?Gln?Asn?Gly?Asp?Glu

645?????????????????650?????????????????655Ala?Trp?Gly?Asp?Asn?Phe?Ile?Ile?Leu?Glu?Ile?Ser?Pro?Ser?Glu?Lys

660??????????????????665????????????????670Leu?Leu?Ser?Pro?Glu?Leu?Ile?Asn?Thr?Asn?Asn?Trp?Thr?Ser?Thr?Gly

675?????????????????680?????????????????685Ser?Thr?Asn?Ile?Ser?Gly?Asn?Thr?Leu?Thr?Leu?Tyr?Gln?Gly?Gly?Arg

690?????????????????695?????????????????700Gly?Ile?Leu?Lys?Gln?Asn?Leu?Gln?Leu?Asp?Ser?Phe?Ser?Thr?Tyr?Arg705?????????????????710?????????????????715?????????????????720Val?Tyr?Phe?Ser?Val?Ser?Gly?Asp?Ala?Asn?Val?Arg?Ile?Arg?Asn?Ser

725?????????????????730?????????????????735Arg?Glu?Val?Leu?Phe?Glu?Lys?Arg?Tyr?Met?Ser?Gly?Ala?Lys?Asp?Val

740?????????????????745?????????????????750Ser?Glu?Met?Phe?Thr?Thr?Lys?Phe?Glu?Lys?Asp?Asn?Phe?Tyr?Ile?Glu

755?????????????????760?????????????????765Leu?Ser?Gln?Gly?Asn?Asn?Leu?Tyr?Gly?Gly?Pro?Ile?Val?His?Phe?Tyr

770?????????????????775?????????????????780Asp?Val?Ser?Ile?Lys?Xaa?Asp?Arg?Asp?Leu?Ile?Leu?Thr?Val?Phe?Lys785?????????????????790?????????????????795?????????????????800Ser?Xaa?Phe?Leu?Tyr?Asn?Val?Leu?Asp

805

(2) about the information of SEQ ID NO:53

(ⅰ) sequence signature

(A) length: 3474 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: DNA

(ⅲ) the false plan: not

(ⅳ) antisense: not

(ⅵ) primary source

(A) biology: the optimized synthetic BT Cry I A of the corn of purifying (b) gene

( ⅸ ) ：SEQ ID NO:53:ATGGACAACA ACCCCAACAT CAACGAGTGC ATCCCCTACA ACTGCCTGAG CAACCCCGAG60GTGGAGGTGC TGGGCGGCGA GCGCATCGAG ACCGGCTACA CCCCCATCGA CATCAGCCTG120AGCCTGACCC AGTTCCTGCT GAGCGAGTTC GTGCCCGGCG CCGGCTTCGT GCTGGGCCTG180GTGGACATCA TCTGGGGCAT CTTCGGCCCC AGCCAGTGGG ACGCCTTCCT GGTGCAGATC240GAGCAGCTGA TCAACCAGCG CATCGAGGAG TTCGCCCGCA ACCAGGCCAT CAGCCGCCTG300GAGGGCCTGA GCAACCTGTA CCAGATCTAC GCCGAGAGCT TCCGCGAGTG GGAGGCCGAC360CCCACCAACC CCGCCCTGCG CGAGGAGATG CGCATCCAGT TCAACGACAT GAACAGCGCC420CTGACCACCG CCATCCCCCT GTTCGCCGTG CAGAACTACC AGGTGCCCCT GCTGAGCGTG480TACGTGCAGG CCGCCAACCT GCACCTGAGC GTGCTGCGCG ACGTGAGCGT GTTCGGCCAG540CGCTGGGGCT TCGACGCCGC CACCATCAAC AGCCGCTACA ACGACCTGAC CCGCCTGATC600GGCAACTACA CCGACCACGC CGTGCGCTGG TACAACACCG GCCTGGAGCG CGTGTGGGGC660CCCGACAGCC GCGACTGGAT CCGCTACAAC CAGTTCCGCC GCGAGCTGAC CCTGACCGTG720CTGGACATCG TGAGCCTGTT CCCCAACTAC GACAGCCGCA CCTACCCCAT CCGCACCGTG780AGCCAGCTGA CCCGCGAGAT CTACACCAAC CCCGTGCTGG AGAACTTCGA CGGCAGCTTC840CGCGGCAGCG CCCAGGGCAT CGAGGGCAGC ATCCGCAGCC CCCACCTGAT GGACATCCTG900AACAGCATCA CCATCTACAC CGACGCCCAC CGCGGCGAGT ACTACTGGAG CGGCCACCAG960ATCATGGCCA GCCCCGTGGG CTTCAGCGGC CCCGAGTTCA CCTTCCCCCT GTACGGCACC1020ATGGGCAACG CCGCCCCCCA GCAGCGCATC GTGGCCCAGC TGGGCCAGGG CGTGTACCGC1080ACCCTGAGCA GCACCCTGTA CCGCCGCCCC TTCAACATCG GCATCAACAA CCAGCAGCTG1140AGCGTGCTGG ACGGCACCGA GTTCGCCTAC GGCACCAGCA GCAACCTGCC CAGCGCCGTG1200TACCGCAAGA GCGGCACCGT GGACAGCCTG GACGAGATCC CCCCCCAGAA CAACAACGTG1260CCCCCCCGCC AGGGCTTCAG CCACCGCCTG AGCCACGTGA GCATGTTCCG CAGCGGCTTC1320AGCAACAGCA GCGTGAGCAT CATCCGCGCC CCCATGTTCA GCTGGATCCA CCGCAGCGCC1380GAGTTCAACA ACATCATCCC CAGCAGCCAG ATCACCCAGA TCCCCCTGAC CAAGAGCACC1440AACCTGGGCA GCGGCACCAG CGTGGTGAAG GGCCCCGGCT TCACCGGCGG CGACATCCTG1500CGCCGCACCA GCCCCGGCCA GATCAGCACC CTGCGCGTGA ACATCACCGC CCCCCTGAGC1560CAGCGCTACC GCGTGCGCAT CCGCTACGCC AGCACCACCA ACCTGCAGTT CCACACCAGC1620ATCGACGGCC GCCCCATCAA CCAGGGCAAC TTCAGCGCCA CCATGAGCAG CGGCAGCAAC1680CTGCAGAGCG GCAGCTTCCG CACCGTGGGC TTCACCACCC CCTTCAACTT CAGCAACGGC1740AGCAGCGTGT TCACCCTGAG CGCCCACGTG TTCAACAGCG GCAACGAGGT GTACATCGAC1800CGCATCGAGT TCGTGCCCGC CGAGGTGACC TTCGAGGCCG AGTACGACCT GGAGCGCGCC1860CAGAAGGCCG TGAACGAGCT GTTCACCAGC AGCAACCAGA TCGGCCTGAA GACCGACGTG1920ACCGACTACC ACATCGACCA GGTGAGCAAC CTGGTGGAGT GCCTGAGCGA CGAGTTCTGC1980CTGGACGAGA AGAAGGAGCT GAGCGAGAAG GTGAAGCACG CCAAGCGCCT GAGCGACGAG2040CGCAACCTGC TGCAGGACCC CAACTTCCGC GGCATCAACC GCCAGCTGGA CCGCGGCTGG2100CGCGGCAGCA CCGACATCAC CATCCAGGGC GGCGACGACG TGTTCAAGGA GAACTACGTG2160ACCCTGCTGG GCACCTTCGA CGAGTGCTAC CCCACCTACC TGTACCAGAA GATCGACGAG2220AGCAAGCTGA AGGCCTACAC CCGCTACCAG CTGCGCGGCT ACATCGAGGA CAGCCAGGAC2280CTGGAGATCT ACCTGATCCG CTACAACGCC AAGCACGAGA CCGTGAACGT GCCCGGCACC2340GGCAGCCTGT GGCCCCTGAG CGCCCCCAGC CCCATCGGCA AGTGCGCCCA CCACAGCCAC2400CACTTCAGCC TGGACATCGA CGTGGGCTGC ACCGACCTGA ACGAGGACCT GGGCGTGTGG2460GTGATCTTCA AGATCAAGAC CCAGGACGGC CACGCCCGCC TGGGCAACCT GGAGTTCCTG2520GAGGAGAAGC CCCTGGTGGG CGAGGCCCTG GCCCGCGTGA AGCGCGCCGA GAAGAAGTGG2580CGCGACAAGC GCGAGAAGCT GGAGTGGGAG ACCAACATCG TGTACAAGGA GGCCAAGGAG2640AGCGTGGACG CCCTGTTCGT GAACAGCCAG TACGACCGCC TGCAGGCCGA CACCAACATC2700GCCATGATCC ACGCCGCCGA CAAGCGCGTG CACAGCATCC GCGAGGCCTA CCTGCCCGAG2760CTGAGCGTGA TCCCCGGCGT GAACGCCGCC ATCTTCGAGG AGCTGGAGGG CCGCATCTTC2820ACCGCCTTCA GCCTGTACGA CGCCCGCAAC GTGATCAAGA ACGGCGACTT CAACAACGGC2880CTGAGCTGCT GGAACGTGAA GGGCCACGTG GACGTGGAGG AGCAGAACAA CCACCGCAGC2940GTGCTGGTGG TGCCCGAGTG GGAGGCCGAG GTGAGCCAGG AGGTGCGCGT GTGCCCCGGC3000CGCGGCTACA TCCTGCGCGT GACCGCCTAC AAGGAGGGCT ACGGCGAGGG CTGCGTGACC3060ATCCACGAGA TCGAGAACAA CACCGACGAG CTGAAGTTCA GCAACTGCGT GGAGGAGGAG3120GTGTACCCCA ACAACACCGT GACCTGCAAC GACTACACCG CCACCCAGGA GGAGTACGAG3180GGCACCTACA CCAGCCGCAA CCGCGGCTAC GACGGCGCCT ACGAGAGCAA CAGCAGCGTG3240CCCGCCGACT ACGCCAGCGC CTACGAGGAG AAGGCCTACA CCGACGGCCG CCGCGACAAC3300CCCTGCGAGA GCAACCGCGG CTACGGCGAC TACACCCCCC TGCCCGCCGG CTACGTGACC3360AAGGAGCTGG AGTACTTCCC CGAGACCGAC AAGGTGTGGA TCGAGATCGG CGAGACCGAG3420GGCACCTTCA TCGTGGACAG CGTGGAGCTG CTGCTGATGG AGGAGTAGTA CATG3474

(2) about the information of SEQ ID NO:54

(ⅰ) sequence signature

(A) length: 3508 nucleotide

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: DNA

(ⅳ) antisense: not

(ⅵ) primary source

(A) biology: the optimized total length BT Cry of synthetic corn I A (b) gene

( ⅹⅰ ) ：SEQ ID NO:54:GATCCAACAA TGGACAACAA CCCCAACATC AACGAGTGCA TCCCCTACAA CTGCCTGAGC60AACCCCGAGG TGGAGGTGCT GGGCGGCGAG CGCATCGAGA CCGGCTACAC CCCCATCGAC120ATCAGCCTGA GCCTGACCCA GTTCCTGCTG AGCGAGTTCG TGCCCGGCGC CGGCTTCGTG180CTGGGCCTGG TGGACATCAT CTGGGGCATC TTCGGCCCCA GCCAGTGGGA CGCCTTCCTG240GTGCAGATCG AGCAGCTGAT CAACCAGCGC ATCGAGGAGT TCGCCCGCAA CCAGGCCATC300AGCCGCCTGG AGGGCCTGAG CAACCTGTAC CAAATCTACG CCGAGAGCTT CCGCGAGTGG360GAGGCCGACC CCACCAACCC CGCCCTGCGC GAGGAGATGC GCATCCAGTT CAACGACATG420AACAGCGCCC TGACCACCGC CATCCCCCTG TTCGCCGTGC AGAACTACCA GGTGCCCCTG480CTGAGCGTGT ACGTGCAGGC CGCCAACCTG CACCTGAGCG TGCTGCGCGA CGTCAGCGTG540TTCGGCCAGC GCTGGGGCTT CGACGCCGCC ACCATCAACA GCCGCTACAA CGACCTGACC600CGCCTGATCG GCAACTACAC CGACCACGCC GTGCGCTGGT ACAACACCGG CCTGGAGCGC660GTGTGGGGTC CCGACAGCCG CGACTGGATC AGGTACAACC AGTTCCGCCG CGAGCTGACC720CTGACCGTGC TGGACATCGT GAGCCTGTTC CCCAACTACG ACAGCCGCAC CTACCCCATC780CGCACCGTGA GCCAGCTGAC CCGCGAGATT TACACCAACC CCGTGCTGGA GAACTTCGAC840GGCAGCTTCC GCGGCAGCGC CCAGGGCATC GAGGGCAGCA TCCGCAGCCC CCACCTGATG900GACATCCTGA ACAGCATCAC CATCTACACC GACGCCCACC GCGGCGAGTA CTACTGGAGC960GGCCACCAGA TCATGGCCAG CCCCGTCGGC TTCAGCGGCC CCGAGTTCAC CTTCCCCCTG1020TACGGCACCA TGGGCAACGC TGCACCTCAG CAGCGCATCG TGGCACAGCT GGGCCAGGGA1080GTGTACCGCA CCCTGAGCAG CACCCTGTAC CGTCGACCTT TCAACATCGG CATCAACAAC1140CAGCAGCTGA GCGTGCTGGA CGGCACCGAG TTCGCCTACG GCACCAGCAG CAACCTGCCC1200AGCGCCGTGT ACCGCAAGAG CGGCACCGTG GACAGCCTGG ACGAGATCCC CCCTCAGAAC1260AACAACGTGC CACCTCGACA GGGCTTCAGC CACCGTCTGA GCCACGTGAG CATGTTCCGC1320AGTGGCTTCA GCAACAGCAG CGTGAGCATC ATCCGTGCAC CTATGTTCAG CTGGATTCAC1380CGCAGTGCCG AGTTCAACAA CATCATCCCC AGCAGCCAGA TCACCCAGAT CCCCCTGACC1440AAGAGCACCA ACCTGGGCAG CGGCACCAGC GTGGTGAAGG GCCCCGGCTT CACCGGCGGC1500GACATCCTGC GCCGCACCAG CCCCGGCCAG ATCAGCACCC TGCGCGTGAA CATCACCGCC1560CCCCTGAGCC AGCGCTACCG CGTCCGCATC CGCTACGCCA GCACCACCAA CCTGCAGTTC1620CACACCAGCA TCGACGGCCG CCCCATCAAC CAGGGCAACT TCAGCGCCAC CATGAGCAGC1680GGCAGCAACC TGCAGAGCGG CAGCTTCCGC ACCGTGGGCT TCACCACCCC CTTCAACTTC1740AGCAACGGCA GCAGCGTGTT CACCCTGAGC GCCCACGTGT TCAACAGCGG CAACGAGGTG1800TACATCGACC GCATCGAGTT CGTGCCCGCC GAGGTGACCT TCGAGGCCGA GTACGACCTG1860GAGAGGGCTC AGAAGGCCGT GAACGAGCTG TTCACCAGCA GCAACCAGAT CGGCCTGAAG1920ACCGACGTGA CCGACTACCA CATCGATCAG GTGAGCAACC TGGTGGAGTG CCTGAGCGAC1980GAGTTCTGCC TGGACGAGAA GAAGGAGCTG AGCGAGAAGG TGAAGCACGC CAAGCGCCTG2040AGCGACGAGC GCAACCTGCT GCAGGACCCC AACTTCCGCG GCATCAACCG CCAGCTGGAC2100CGCGGCTGGC GCGGCAGCAC CGACATCACC ATCCAGGGCG GCGACGACGT GTTCAAGGAG2160AACTACGTGA CCCTGCTGGG CACCTTCGAC GAGTGCTACC CCACCTACCT GTACCAGAAG2220ATCGACGAGA GCAAGCTGAA GGCCTACACC CGCTACCAGC TGCGCGGCTA CATCGAGGAC2280AGCCAGGACC TGGAGATCTA CCTGATCCGC TACAACGCCA AGCACGAGAC CGTGAACGTG2340CCCGGCACCG GCAGCCTGTG GCCCCTGAGC GCCCCCAGCC CCATCGGCAA GTGCGCCCAC2400CACAGCCACC ACTTCAGCCT GGACATCGAC GTGGGCTGCA CCGACCTGAA CGAGGACCTG2460GGCGTGTGGG TGATCTTCAA GATCAAGACC CAGGACGGCC ACGCCCGCCT GGGCAACCTG2520GAGTTCCTGG AGGAGAAGCC CCTGGTGGGC GAGGCCCTGG CCCGCGTGAA GCGCGCCGAG2580AAGAAGTGGC GCGACAAGCG CGAGAAGCTG GAGTGGGAGA CCAACATCGT GTACAAGGAG2640GCCAAGGAGA GCGTGGACGC CCTGTTCGTG AACAGCCAGT ACGACCGCCT GCAGGCCGAC2700ACCAACATCG CCATGATCCA CGCCGCCGAC AAGCGCGTGC ACAGCATTCG CGAGGCCTAC2760CTGCCCGAGC TGAGCGTGAT CCCCGGCGTG AACGCCGCCA TCTTCGAGGA GCTGGAGGGC2820CGCATCTTCA CCGCCTTCAG CCTGTACGAC GCCCGCAACG TGATCAAGAA CGGCGACTTC2880AACAACGGCC TGAGCTGCTG GAACGTGAAG GGCCACGTGG ACGTGGAGGA GCAGAACAAC2940CACCGCAGCG TGCTGGTGGT GCCCGAGTGG GAGGCCGAGG TGAGCCAGGA GGTGCGCGTG3000TGCCCCGGCC GCGGCTACAT CCTGCGCGTG ACCGCCTACA AGGAGGGCTA CGGCGAGGGC3060TGCGTGACCA TCCACGAGAT CGAGAACAAC ACCGACGAGC TCAAGTTCAG CAACTGCGTG3120GAGGAGGAGG TGTACCCCAA CAACACCGTG ACCTGCAACG ACTACACCGC CACCCAGGAG3180GAGTACGAGG GCACCTACAC CAGCCGCAAC CGCGGCTACG ACGGCGCCTA CGAGAGCAAC3240AGCAGCGTGC CCGCCGACTA CGCCAGCGCC TACGAGGAGA AGGCCTACAC CGACGGCCGC3300CGCGACAACC CCTGCGAGAG CAACCGCGGC TACGGCGACT ACACCCCCCT GCCCGCCGGC3360TACGTGACCA AGGAGCTGGA GTACTTCCCC GAGACCGACA AGGTGTGGAT CGAGATCGGC3420GAGACCGAGG GCACCTTCAT CGTGGACAGC GTGGAGCTGC TGCTGATGGA GGAGTAGTAC3480ATGTGATAGT ACGTAAGCTC GAGGATCT 3508

(2) about the information of SEQ ID NO:55

(ⅰ) sequence signature

(A) length: 1961 base-pairs

(B) type: nucleic acid

(C) chain: strand

(D) topological structure: line style

(ⅱ) molecule type: DNA

(ⅲ) the false plan: not

(ⅳ) antisense: not

(ⅵ) primary source

(A) biology: synthetic corn optimization BT Cry I A (b) gene of brachymemma

( ⅹⅰ ) ：SEQ ID NO:55:GATCCAACAA TGGACAACAA CCCCAACATC AACGAGTGCA TCCCCTACAA CTGCCTGAGC 60AACCCCGAGG TGGAGGTGCT GGGCGGCGAG CGCATCGAGA CCGGCTACAC CCCCATCGAC 120ATCAGCCTGA GCCTGACCCA GTTCCTGCTG AGCGAGTTCG TGCCCGGCGC CGGCTTCGTG 180CTGGGCCTGG TGGACATCAT CTGGGGCATC TTCGGCCCCA GCCAGTGGGA CGCCTTCCTG 240GTGCAGATCG AGCAGCTGAT CAACCAGCGC ATCGAGGAGT TCGCCCGCAA CCAGGCCATC 300AGCCGCCTGG AGGGCCTGAG CAACCTGTAC CAAATCTACG CCGAGAGCTT CCGCGAGTGG 360GAGGCCGACC CCACCAACCC CGCCCTGCGC GAGGAGATGC GCATCCAGTT CAACGACATG 420AACAGCGCCC TGACCACCGC CATCCCCCrG TTCGCCGTGC AGAACTACCA GGTGCCCCTG 480CTGAGCGTGT ACGTGCAGGC CGCCAACCTG CACCTGAGCG TGCTGCGCGA CGTCAGCGTG 540TTCGGCCAGC GCTGGGGCTT CGACGCCGCC ACCATCAACA GCCGCTACAA CGACCTGACC 600CGCCTGATCG GCAACTACAC CGACCACGCC GTGCGCTGGT ACAACACCGG CCTGGAGCGC 660GTGTTGGGTC CCGACAGCCG CGACTGGATC AGGTACAACC AGTTCCGCCG CGAGCTGACC 720CTGACCGTGC TGGACATCGT GAGCCTGTTC CCCAACTACG ACAGCCGCAC CTACCCCATC 780CGCACCGTGA GCCAGCTGAC CCGCGAGATT TACACCAACC CCGTGCTGGA GAACTTCGAC 840GGCAGCTTCC GCGGCAGCGC CCAGGGCATC GAGGGCAGCA TCCGCAGCCC CCACCTGATG 900GACATCCTGA ACAGCATCAC CATCTACACC GACGCCCACC GCGGCGAGTA CTACTGGAGC 960GGCCACCAGA TCATGGCCAG CCCCGTCGGC TTCAGCGGCC CCGAGTTCAC CTTCCCCCTG 1020TACGGCACCA TGGGCAACGC TGCACCTCAG CAGCGCATCG TGGCACAGCT GGGCCAGGGA 1080GTGTACCGCA CCCTGAGCAG CACCCTGTAC CGTCGACCTT TCAACATCGG CATCAACAAC 1140CAGCAGCTGA GCGTGCTGGA CGGCACCGAG TTCGCCTACG GCACCAGCAG CAACCTGCCC 1200AGCGCCGTGT ACCGCAAGAG CGGCACCGTG GACAGCCTGG ACGAGATCCC CCCTCAGAAC 1260AACAACGTGC CACCTCGACA GGGCTTCAGC CACCGTCTGA GCCACGTGAG CATGTTCCGC 1320AGTGGCTTCA GCAACAGCAG CGTGAGCATC ATCCGTGCAC CTATGTTCAG CTGGATTCAC 1380CGCAGTGCCG AGTTCAACAA CATCATCCCC AGCAGCCAGA TCACCCAGAT CCCCCTGACC 1440AAGAGCACCA ACCTGGGCAG CGGCACCAGC GTGGTGAAGG GCCCCGGCTT CACCGGCGGC 1500GACATCCTGC GCCGCACCAG CCCCGGCCAG ATCAGCACCC TGCGCGTGAA CATCACCGCC 1560CCCCTGAGCC AGCGCTACCG CGTCCGCATC CGCTACGCCA GCACCACCAA CCTGCAGTTC 1620CACACCAGCA TCGACGGCCG CCCCATCAAC CAGGGCAACT TCAGCGCCAC CATGAGCAGC 1680GGCAGCAACC TGCAGAGCGG CAGCTTCCGC ACCGTGGGCT TCACCACCCC CTTCAACTTC 1740AGCAACGGCA GCAGCGTGTT CACCCTGAGC GCCCACGTGT TCAACAGCGG CAACGAGGTG 1800TACATCGACC GCATCGAGTT CGTGCCCGCC GAGGTGACCT TCGAGGCCGA GTACGACCTG 1860GAGAGGGCTC AGAAGGCCGT GAACGAGCTG TTCACCAGCA GCAACCAGAT CGGCCTGAAG 1920ACCGACGTGA CCGACTACCA CATCGATCAG GTGTAGGAGC T 1961

In agricultural research institute preservation center (NRRL), Northern Regional ResearchCenter, 1815 North Universlty Street, Peoria, lllinois 61604, U.S.A.

Bacterial strain is named preserving number preservation date Bacillus cercus AB78 NRRLB-21058 Escherichia coli pCIB4431 on the 18th NRRLB-18998 March in 1993 on September 21st, 1992

Claims (42)

1. the method for the infringement that caused by Ostrinia furnacalis kind is avoided in protective plant and filial generation thereof, comprises directly or indirectly to plant or plant seed or the plant growing Zoned application toxin protein as the bacillus kind of active ingredient.

2. method according to claim 1, toxin protein wherein are the toxin proteins of bacillus thuringiensis.

3. method according to claim 1, toxin protein wherein are the toxin proteins of Bacillus cercus.

4. method according to claim 1, toxin protein wherein are Cry type albumen, preferably Cry I type albumen, more preferably Cry I A type albumen, most preferably Cry I A (b) albumen.

5. method according to claim 4, toxin protein wherein are the Cry I type albumen according to SEQ ID Nos53-55.

6. method according to claim 1, toxin protein wherein is a VIP type albumen, and VIP1 type albumen preferably is as VIP1A (a) albumen or VIP1A (b) albumen, or VIP2 type albumen, as the combination of VIP2A (a) albumen or VIP2A (b) albumen or VIP1 type albumen and VIP2 type albumen.

7. method according to claim 1, wherein toxin protein is a VIP3 type albumen, as VIP3A (a) albumen or VIP3A (b) albumen.

8. according to any described method of claim 6-7, wherein toxin protein is according to SEQ ID Nos.1,2, and 4-7,17-24,26-32,35,36,39,40,42,43,45,46,49,50,51 or 52 VIP type albumen.

9. method according to claim 1, wherein toxin protein is administered to plant with the form of insecticidal mixtures.

10. method according to claim 9, wherein insecticidal mixtures comprises microorganism.

11. according to claim 1 or 9 described methods, wherein toxin protein is a Cry I type albumen.

12. method according to claim 9, wherein insecticidal mixtures comprises at least a and suitable carrier-bound Cry I type toxin protein or microorganism, preferably comprises the bacillus thuringiensis and/or the Bacillus cercus of the gene of at least one above-mentioned toxin protein of encoding.

13. method according to claim 12, wherein microorganism is the naturally occurring biology that comprises the Cry I type toxin gene of at least one toxin-encoding albumen.

14. method according to claim 10, wherein microorganism is to comprise at least one Cry type toxin gene, Cry I type toxin gene preferably, more preferably Cry I A type toxin gene and the most preferably naturally occurring biology of Cry I A (b) toxin gene.

15. method according to claim 12, wherein microorganism is the reorganization biology with the Cry I type toxin gene of recombinant forms that comprises at least one toxin-encoding albumen.

16. method according to claim 10, wherein microorganism is to comprise at least one Cry type toxin gene, preferably Cry I type toxin gene, more preferably Cry I A type toxin gene, most preferably the reorganization biology of Cry I A (b) toxin gene.

17. method according to claim 10, wherein insecticidal mixtures comprises at least a and suitable carrier-bound VIP type toxin protein or microorganism, preferably comprises the bacillus thuringiensis and/or the Bacillus cercus of the gene of at least one above-mentioned toxin protein of encoding.

18. method according to claim 10, wherein microorganism is the VIP type protein gene that comprises at least one toxin-encoding albumen, preferably the naturally occurring biology of VIP1A (a) albumen or VIP1A (b) protein gene or VIP2 type protein gene such as VIP2A (a) albumen or VIP2A (b) protein gene.

19. method according to claim 10, wherein microorganism is the naturally occurring biology that comprises VIP3 type protein gene such as VIP3A (a) albumen or VIP3A (b) protein gene of at least one toxin-encoding albumen.

20. according to any described method of claim 17-19, wherein toxin protein is according to SEQ ID Nos.1,2, and 4-7,17-24,26-32,35,36,39,40,42,43,45,46,49,50,51 or 52 Cry type albumen.

21. according to any described method of claim 11-16, wherein toxin protein is the Cry type albumen according to SEQ ID Nos.53-55.

22. according to claim 17-21 any one described, wherein microorganism is that reorganization is biological.

23. according to the described method of claim 1-22, wherein toxin protein transforms plant by the toxin gene with coding bacillus kind toxin protein, and be administered to plant indirectly, and the such plant transformed of ecological region planting that may occur at insect pest enough to provide scale at the control of Ostriniafurnacalis (Asiatic corn borer) kind to reach above-mentioned toxin protein.

24. method according to claim 23, the wherein toxin protein of toxin gene coding bacillus thuringiensis or Bacillus cercus.

25. method according to claim 23, toxin gene coding Cry type toxin protein wherein, Cry I type albumen preferably, Cry I A type albumen more preferably, Cry I A (b) albumen most preferably is as providing in SEQ ID Nos.53-55.

26. method according to claim 23, toxin gene coding VIP type albumen wherein, VIP1 type albumen preferably, as VIP1A (a) albumen or VIP1A (b) albumen, or VIP2 type albumen, as VIP2A (a) albumen or VIP2A (b) albumen.

27. method according to claim 23, toxin gene coding VIP3 type albumen wherein is as VIP3A (a) albumen or VIP3A (b) albumen.

28. according to the described method of claim 23-27, wherein toxin gene is the gene that synthesizes, its codon usage is by using most preferred codon optimization in plant.

29. according to claim 23 and 28 described methods, toxin gene coding Cry I type albumen wherein.

30. according to any described method of claim 23-27, wherein toxin gene is encoded according to SEQ ID Nos.1, and 2,4-7,17-24,26-32,35,36,39,40,42,43,45,46,50,51 or 52 VIP type albumen.

31. according to any described method of claim 1-30, wherein claimed plant is a cereal.

32. method according to claim 31, wherein claimed plant is a corn.

33. according to any described method of claim 1-32, wherein at least a Cry type toxin protein combines with VIP type toxin protein enough to provide the amount at the control of Asiatic corn borer (Ostrinia furnacalis) directly or indirectly to be administered to plant or plant seed or plant growing zone.

34. according to any described method of claim 1-32, wherein active component is Cry I A (b) albumen.

35. according to any described method of claim 1-32, wherein active component is a VIP type albumen.

36. as the application of the active component that in aforementioned claim, defines, be used for insect pest at crop plant control Ostrinia furnacalis (Asiatic corn borer).

37. according to any described recombinant microorganism of claim 23-30 or the application of genetically modified plants, be used for the insect pest at crop plant control Ostrinia furnacalis (Asiatic corn borer), above-mentioned recombinant microorganism or genetically modified plants comprise can be under medium stringent condition and the Cry type of the corresponding toxin protein of coding or the dna molecular of VIP type gene recombination.

38. comprise the seed of genetically modified plants, and with its reliable operation instruction, be used for the commodity bundle in crop plant control Ostrinia furnacalis (Asiatic corn borer) insect pest, above-mentioned genetically modified plants comprise the toxin gene of coding bacillus thuringiensis toxin protein and enough to provide the scale at the control of Ostrinia furnacalis (Asiatic corn borer) kind to reach above-mentioned toxin protein.

39. comprise seed or the microorganism of genetically modified plants, especially bacillus thuringiensis and/or bacillus cereus strain, and with its reliable operation instruction, be used for commodity bundle in crop plant control Ostrinia furnacalis (Asiatic corn borer) insect pest, above-mentioned genetically modified plants or microorganism comprise at least one coding as preceding claim 1-30, the toxin gene of toxin protein of definition in 32 and 33, and enough to provide the scale at the control of Ostriniafurnacalis (Asiatic corn borer) kind to reach above-mentioned toxin protein.

40. agricultural methods, wherein as claim 23-32 any one described, use the toxin gene comprise coding bacillus kind toxin protein and so that genetically modified plants or its filial generation or the genetically modified seed that reaches above-mentioned albumen at the scale of the control of Ostrinia furnacalis (Asiatic corn borer) kind enough to be provided.

41. according to any described method of claim 18-26, wherein at least a Cry type toxin protein is expressed in claimed plant enough to provide at the amount of the control of eating into the insect pest of stem Noctua with VIP type toxin protein.

42. a composition comprises at least a Cry type toxin protein of insect effective dose extremely and VIP type toxin protein as active component, and combines with acceptable carrier on the agronomy.