CN1310952A - Hericium erinaceus noodles and industrialized liquid fermentation producing technology for Hericium erinaceus baterial strain - Google Patents
Hericium erinaceus noodles and industrialized liquid fermentation producing technology for Hericium erinaceus baterial strain Download PDFInfo
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- CN1310952A CN1310952A CN00135442A CN00135442A CN1310952A CN 1310952 A CN1310952 A CN 1310952A CN 00135442 A CN00135442 A CN 00135442A CN 00135442 A CN00135442 A CN 00135442A CN 1310952 A CN1310952 A CN 1310952A
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- hericium erinaceus
- liquid
- jar
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- bacterial strain
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Abstract
The present invention relates to noodle containing heagehogh fungus component and hedgehogh fungus strain liquid fermentation technology. It is characterized in that; (1). 30% hedgehoft fungus fermentation liquid is added into wheat flour; (2). strain fermentation technology includes four step: fungus species selection, sllant culture, liquid species culture and liquid fermentation production, in which the liquid fermentation technology adopts 1st, 2nd species tank and 3rd fermentation tank production. Advantages: containuous production, short production period, the hedgehogh fungus noodle can enhance the immune power of human body etc.
Description
The present invention relates to a kind of alimentary paste and industrialized liquid fermentation producing technology for Hericium erinaceus baterial strain.
Hericium erinaceus has another name called hedgehog hydnum, hedgehog hydnum eats, and is famous edible, medicinal fungi, is listed as one of mountain delicacy.Hericium erinaceus is nutritious, is rich in the amino acid of needed by human, and the record of " aid digestion, sharp the five internal organs " is just arranged on the ancient book.The effect that it has tangible active anticancer and strengthens immunity of organisms especially has significant curative effect to chronic gastritis, cancer of the stomach, duodenal ulcer, is a kind of good medicine for the treatment of disease of digestive system.
But the available technology adopting substituting stuff cultivation, the production cycle is long, and unstable product quality.
The objective of the invention is to: the method that provides a kind of Hericium erinaceus alimentary paste and industrial liquid fermentation technology with short production cycle, that output is high, steady quality, productivity effect are high to produce Hericium erinaceus RCEF772 bacterial strain.
Its goal of the invention realizes by following proposal.
Hericium erinaceus noodles, the raw materials used flour that comprises is characterized in that: add 30% Hericium erinaceus RCEF772 bacterial strain fermentation liquor in flour.
The Hericium erinaceus RCEF772 industrial liquid fermentation producing technology for bacterial strain of described Hericium erinaceus noodles is characterized in that:
1, strain separating
The Hericium erinaceus bacterial strain, the fructification of separating the Hericium erinaceus of arrogant other mountain area robust growth:
2, slant strains is cultivated
The bacterial classification inoculation of separation and purification in the PDA inclined-plane, is put into incubator, and 25 ℃ of cultivations treat that mycelia covers with the inclined-plane, can change over to shake bottle and cultivate;
3, liquid seeds is cultivated
With culture medium pack into 250ml or 500ml triangular flask, every bottled amount is respectively 100ml or 200ml, 1.5Pa/Kg autoclaving 30min, when the nutrient solution after the sterilization is cooled to 37～42 ℃, insert slant strains, inoculum concentration is that 250ml connects 1,500ml connects 2, inoculation is placed on the constant-temperature shaking culture case, and 24～26 ℃, 180rpm cultivates 13d;
Described liquid seed culture medium is: 5% wheat bran liquor, 2% glucose, 1% peptone, 0.2%KH
2PO
4, 0.12%MgSO
4, 0.2% (NH
4)
2SO
4With 91.48% water, pH6.0～7.0.
4, three grade fermemtation is adopted in liquid fermentation production
(1), first class seed pot production
The 50L liquid medium of in the 70L airlift fermentor, packing into, and add defoamer polyoxypropylene ethylene oxide glycerin ether, consumption is 0.03%, 14.7 * 10
4Under the Pa pressure, pressurize 30min inserts cultivation with shake-flask seed after the sterilization cooling, inoculum concentration 10%, and 24～26 ℃ of cultivation temperature, throughput 1: 05 is 4.903～7.0845 * 10
4Under the pa pressure condition, cultivate 60～72h;
(2), the secondary seed jar is produced
The 500L liquid medium of packing in the 700L airlift fermentor when first order seed reaches the logarithmic growth after date, inserts the secondary seed jar, and its process conditions are with the one-level seeding tank, incubation time 60～72h;
(3), the three grade fermemtation jar is produced
The 5T liquid medium of packing in the 7T airlift fermentor at 121 ℃, behind the pressurize 30min, is cooled to 24～26 ℃, inserts the cultured liquid seeds of secondary seed jar, inoculum concentration 10%, and ventilation is cultivated, throughput 1: 0.4～0.5, tank pressure 4.9033 * 10
4Pa cultivates 72～96h;
When the zymotic fluid residual sugar below 1%, zymotic fluid is than thickness, notices whether mycelia attenuates during microscopy in addition, has or not tangible vacuole in the mycelium, and the mycelia that has or not obvious fracture in the zymotic fluid, if said circumstances has one to several appearance, can go out jar, get Hericium erinaceus RCEF772 bacterial strain fermentation liquor.
Liquid Culture based formulas in described I and II seeding tank, the production of three grade fermemtation jar is: 5% wheat bran liquor, 1% white granulated sugar, 3% analysis for soybean powder, 1% yeast, 0.2%KH
2PO
4, 0.12%MgSO
4, 0.2% (NH
4)
2SO
4With 90.48% water, pH6.0～7.0.
Hericium erinaceus health caring noodles of the present invention not only makes delicious taste and the nutritional labeling of Hericium erinaceus be kept, and medicine-food two-purpose, can improve body immunity, and can reach food therapy effect to disease of digestive system.
Industrialized liquid fermentation producing technology for Hericium erinaceus baterial strain can be produced continuously, is not subjected to restrictions such as production site, season.With short production cycle, constant product quality, and can go up scale, go out benefit.Simultaneously, the bioactivator in the zymotic fluid as: polysaccharide, polypeptide, alkaloid, terpenoid etc. can both obtain further comprehensive development and utilization.
Below by embodiment the present invention is done to describe further.
Embodiment:
Hericium erinaceus noodles, raw materials used is flour and Hericium erinaceus RCEF772 bacterial strain fermentation liquor.With face the time, in flour, add 30% Hericium erinaceus RCEF772 bacterial strain fermentation liquor,, be pressed into noodles behind the placement 30min, packing aluminium pool nitrogen-filled packaging after the low temperature drying flour and even.
The Hericium erinaceus RCEF772 industrial liquid fermentation producing technology for bacterial strain of described Hericium erinaceus noodles, being divided into is four big steps.
1, strain separating
The Hericium erinaceus bacterial strain, the fructification of separating the Hericium erinaceus of arrogant other mountain area robust growth;
2, slant strains is cultivated
The bacterial classification inoculation of separation and purification in the PDA inclined-plane, is put into incubator, and 25 ℃ of cultivations treat that mycelia covers with the inclined-plane, can change over to shake bottle and cultivate.
3, liquid seeds is cultivated
With culture medium pack into 250ml or 500ml triangular flask, every bottled amount is respectively 100ml or 200ml, 1.5Pa/Kg autoclaving 30min, when the nutrient solution after the sterilization is cooled to 37～42 ℃, insert slant strains, inoculum concentration is that 250ml connects 1,500ml connects 2, inoculation is placed on the constant-temperature shaking culture case, and 24～26 ℃, 180rpm cultivates 3d.
Described culture medium is 5% wheat bran liquor, 2% glucose, 1% peptone, 0.2%KH
2PO
4, 0.12%MgSO
4, 0.2% (NH
4)
2SO
4With 91.48% water, pH6.0～7.0.
4, three grade fermemtation is adopted in liquid fermentation production
(1), first class seed pot production
The 50L liquid medium of packing in the 70L airlift fermentor, and add defoamer polyoxypropylene ethylene oxide glycerin ether, consumption is 0.03%, 14.7 * 10
4Under the pa pressure, pressurize 30min inserts cultivation with shake-flask seed after the sterilization cooling, inoculum concentration 10%, and 24～26 ℃ of cultivation temperature, throughput 1: 05 is 4.903～7.0845 * 10
4Under the pa condition, pressurize is cultivated through 60～72h ventilation, reaches the logarithmic growth after date, inserts the secondary seed jar.
(2), secondary seed jar bacterial classification is produced
The 500L liquid medium of packing in the 700L airlift fermentor when first order seed reaches the logarithmic growth after date, inserts the secondary seed jar, and its process conditions are with the one-level seeding tank, incubation time 60～72h.
(3), the three grade fermemtation jar is produced
The 5T liquid medium of packing in the 7T airlift fermentor, water temperature is higher than 95 ℃, feeds Steam Heating to 121 ℃, behind the pressurize 30min, be cooled to 24～26 ℃, insert the cultured liquid seeds of secondary seed jar, ventilation is cultivated, throughput 1: 0.4～0.5, tank pressure 4.9033 * 10
4Pa, incubation time 72～96h;
When the zymotic fluid residual sugar below 1%, zymotic fluid is than thickness, notices whether mycelia attenuates during microscopy in addition, has or not tangible vacuole in the mycelium, and the mycelia that has or not obvious fracture in the zymotic fluid, if said circumstances has one to several appearance, can go out jar, get Hericium erinaceus RCEF772 liquid fermentation liquid.
Fluid nutrient medium formula of liquid in described I and II seeding tank, the production of three grade fermemtation jar is: 5% wheat bran liquor, 1% white granulated sugar, 3% analysis for soybean powder, 1% yeast, 0.2%KH
2PO
4, 0.12%MgSO
4, 0.2% (NH
4)
2SO
4With 90.48% water, pH6.0～7.0.
The Hericium erinaceus zymotic fluid not only can be cooked noodles, also can be used for developing beverage products.
Claims (4)
1, Hericium erinaceus noodles comprises flour, it is characterized in that: add 30% Hericium erinaceus RCEF772 bacterial strain fermentation liquor in flour.
2, the Hericium erinaceus RCEF772 industrial liquid fermentation producing technology for bacterial strain of Hericium erinaceus noodles according to claim 1 is characterized in that:
2.1, strain separating
The Hericium erinaceus bacterial strain, the fructification of separating the Hericium erinaceus of arrogant other mountain area robust growth;
2.2, slant strains cultivates
The bacterial classification inoculation of separation and purification in the PDA inclined-plane, is put into incubator, and 25 ℃ of cultivations treat that mycelia covers with the inclined-plane, can change over to shake bottle and cultivate;
2.3, liquid seeds cultivates
With culture medium pack into 250ml or 500ml triangular flask, every bottled amount is respectively 100ml or 200ml, and 1.5Pa/Kg autoclaving 30min when the nutrient solution after the sterilization is cooled to 37～42 ℃, inserts slant strains, and inoculum concentration is 250ml
Connect 1,500ml connects 2, and inoculation is placed on the constant-temperature shaking culture case, and 24～26 ℃, 180rpm cultivates 13d;
2.4, liquid fermentation production, adopt three grade fermemtation
(1), first class seed pot production
The 50L liquid medium of in the 70L airlift fermentor, packing into, and add defoamer polyoxypropylene ethylene oxide glycerin ether, consumption is 0.03%, 14.7 * 10
4Under the Pa pressure, pressurize 30min inserts cultivation with shake-flask seed after the sterilization cooling, inoculum concentration 10%, and 24～26 ℃ of cultivation temperature, throughput 1: 05 is 4.903～7.0845 * 10
4Under the Pa pressure condition, cultivate 60～72h;
(2), the secondary seed jar is produced
The 500L liquid medium of packing in the 700L airlift fermentor when first order seed reaches the logarithmic growth after date, inserts the secondary seed jar, and its process conditions are with the one-level seeding tank, incubation time 60～72h;
(3), the three grade fermemtation jar is produced
The 5T liquid medium of packing in the 7T airlift fermentor at 121 ℃, behind the pressurize 30min, is cooled to 24～26 ℃, inserts the cultured liquid seeds of secondary seed jar, inoculum concentration 10%, and ventilation is cultivated, throughput 1: 0.4～0.5, tank pressure 4.9033 * 10
4Pa cultivates 72～96h;
When the zymotic fluid residual sugar below 1%, zymotic fluid is than thickness; Notice whether mycelia attenuates during microscopy in addition; Have or not tangible vacuole in the mycelium; And the mycelia that has or not obvious fracture in the zymotic fluid, if said circumstances has one to several appearance, can go out jar, get Hericium erinaceus RCEF772 zymotic fluid.
3, the Hericium erinaceus RCEF772 industrial liquid fermentation producing technology for bacterial strain of Hericium erinaceus noodles according to claim 2, it is characterized in that: described liquid seed culture medium is: 5% wheat bran liquor, 2% glucose, 1% peptone, 0.2%KH
2PO
4, 0.12%MgSO
4, 0.2% (NH
4)
2SO
4With 91.48% water, pH6.0～7.0.
4, the Hericium erinaceus RCEF772 industrial liquid fermentation producing technology for bacterial strain of Hericium erinaceus noodles according to claim 2 is characterized in that: the Liquid Culture based formulas in described I and II seeding tank, the production of three grade fermemtation jar is: 5% wheat bran liquor, 1% white granulated sugar, 3% analysis for soybean powder, 1% yeast, 0.2%KH
2PO
4, 0.12%MgSO
4, 0.2% (NH
4)
2SO
4With 90.48% water, pH6.0～7.0.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN00135442A CN1109508C (en) | 2000-12-21 | 2000-12-21 | Hericium erinaceus noodles and industrialized liquid fermentation producing technology for Hericium erinaceus baterial strain |
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|---|---|---|---|
| CN00135442A CN1109508C (en) | 2000-12-21 | 2000-12-21 | Hericium erinaceus noodles and industrialized liquid fermentation producing technology for Hericium erinaceus baterial strain |
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|---|---|
| CN1310952A true CN1310952A (en) | 2001-09-05 |
| CN1109508C CN1109508C (en) | 2003-05-28 |
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|---|---|---|---|
| CN00135442A Expired - Fee Related CN1109508C (en) | 2000-12-21 | 2000-12-21 | Hericium erinaceus noodles and industrialized liquid fermentation producing technology for Hericium erinaceus baterial strain |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102229508A (en) * | 2011-05-28 | 2011-11-02 | 镇江市食用菌研究所 | Hydroponic matrix of Hericium erinaceus |
| CN104757393A (en) * | 2015-04-26 | 2015-07-08 | 哈尔滨伟平科技开发有限公司 | Preparation method of black rich health food |
| CN105495334A (en) * | 2015-12-31 | 2016-04-20 | 钟国正 | Health cultivation longevity noodles and manufacturing method of health cultivation longevity noodles |
| CN105875728A (en) * | 2016-07-01 | 2016-08-24 | 河北大学 | Potato-apricot bread with hericium erinaceus flavor and making method thereof |
| CN106071592A (en) * | 2016-06-29 | 2016-11-09 | 宫晶晶 | The preparation method of a kind of hedgehog hydnum oral liquid and special culture media |
| CN107637395A (en) * | 2017-10-27 | 2018-01-30 | 丘少荣 | A kind of method that cultivation lichee bacterium is starched using monkey mushroom mycelium fermentation |
-
2000
- 2000-12-21 CN CN00135442A patent/CN1109508C/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102229508A (en) * | 2011-05-28 | 2011-11-02 | 镇江市食用菌研究所 | Hydroponic matrix of Hericium erinaceus |
| CN104757393A (en) * | 2015-04-26 | 2015-07-08 | 哈尔滨伟平科技开发有限公司 | Preparation method of black rich health food |
| CN105495334A (en) * | 2015-12-31 | 2016-04-20 | 钟国正 | Health cultivation longevity noodles and manufacturing method of health cultivation longevity noodles |
| CN106071592A (en) * | 2016-06-29 | 2016-11-09 | 宫晶晶 | The preparation method of a kind of hedgehog hydnum oral liquid and special culture media |
| CN105875728A (en) * | 2016-07-01 | 2016-08-24 | 河北大学 | Potato-apricot bread with hericium erinaceus flavor and making method thereof |
| CN107637395A (en) * | 2017-10-27 | 2018-01-30 | 丘少荣 | A kind of method that cultivation lichee bacterium is starched using monkey mushroom mycelium fermentation |
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| Publication number | Publication date |
|---|---|
| CN1109508C (en) | 2003-05-28 |
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Owner name: HEFEI MAIKELUO BIOLOGICAL ENGINEERING CO.,LTD. Free format text: FORMER OWNER: ANHUI LINGYUAN AWETO RESEARCH INSTITUTE Effective date: 20080530 |
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Effective date of registration: 20080530 Address after: Room 128, building 106, Anhui Agriculture University, Anhui, Hefei Patentee after: Hefei Micro Biological Engineering Co., Ltd. Address before: 753 mailbox, Anhui Agriculture University, 130 West Changjiang Road, Hefei, Anhui Patentee before: Anhui Linyuan Chinese Caterpillar Fungus Institute |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20030528 Termination date: 20141221 |
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