CN1307872A - Selenium product of natural marine organism and its preparation - Google Patents

Abstract

The present invention relates to a natural marine biological selenium product and its preparation method. It is characterized by that the fresh scallop edge and mytilus muscle are used as raw material, one of them is processed by homogenizing, centrifugal taking supernatant fluid, adding ammonium sulfate and centrifugal precipitation and dialysis concentration drying to obtain scallope edge extract, and another of them is processed by homogenizing, enzymolysis, decolouring, removing fishy smell, centrifugal taking supernatant, concentrating and drying to obtain mytilus extract, then two extracts are mixed according to a certain proportion, pulverized and seived to obtain said invented product which is used for preventing and auxiliary curing tumor and liver disease.

Description

Selenium product of natural marine organism and preparation method thereof

A kind of selenium product of natural marine organism and preparation method thereof belongs to auxiliary treatment nutritive health products and preparing technical field thereof, is mainly used in prevention and adjuvant therapy of tumors and hepatic disease, slow down aging and enhancing human body immunity ability.

In the prior art, people are for the utilization of scallop, except taking advantage of bright direct eating, just only getting its cylinder is processed into dried dry scallop or canned food, and remaining leftover bits and pieces one scallop edge, the feedstuff that making of having breeded fish, supported shrimp, what have then wastes, not only wherein contained natural marine organism selenium is not utilized, but also can cause environmental pollution.And for the utilization of mussel, except fresh directly edible, also only boil to shell and dry, for edible throughout the year, natural marine organism selenium contained in its meat is not exploited equally.Through entrusting the update search of information centre of the national Ministry of Education, 13 systems such as " SWIC ", " Chinese science technological achievement data base ", " Chinese patent database " and data base have been carried out update search, the result does not see that by the end of at present identical with the present invention or akin technology is arranged, and also finds no identical with the present invention or akin patent application formerly.

The object of the present invention is to provide a kind of abundant raw material to be easy to get, preparation technology is comparatively simple, equipment needed thereby is few, can turn waste into wealth, extract the system pure natural substance, energy prevention and adjuvant therapy of tumors and hepatopathy, defying age does not have selenium product of natural marine organism of any toxic action and preparation method thereof to human body.

According to selenic existence form in the selenic physiological function of trace element and scallop and the mussel, at existing questions in the prior art, basic design of the present invention is: with fresh scallops limit and bright mussel meat is raw material, respectively through homogenate, separate slagging-off and extract supernatant, add the centrifugal again supernatant of removing of ammonium sulfate in the supernatant and leave and take precipitate, concentrated and dry through dialysing again, make scallop edge and extract dry material, with process homogenate, enzymolysis, decoloration deodorization centrifuging and taking supernatant, makes mussel meat extraction dry material through concentrating again with drying; According to a certain percentage two kinds of above-mentioned extraction dry materials are mixed through pulverizing mutually, sieve and make required dosage form, thereby realize goal of the invention.

Selenium product of natural marine organism of the present invention, it is characterized in that these goods extract dry material by the fresh scallops limit through homogenate, extraction, precipitation, dialysis, concentrated, dry gained scallop edge and account for 60～70% of gross weight by weight, extract dry material with bright mussel meat through homogenate, enzymolysis, centrifugal, concentrated, the dry gained mussel of decoloration deodorization and account for 30～40% of gross weight, the powdered mixture that the two mixes mutually, pulverizes, sieves and makes.

The preparation method of the selenium product of natural marine organism that preparation of the present invention is above-mentioned, it is characterized in that this method comprises following process steps: one. extract the scallop edge dry material, it comprises again: 1. get and add the small amounts of phosphoric acid salt buffer after weighing in pure fresh scallops limit, i.e. homogenate in high-speed tissue mashing machine, 2. the phosphate buffer that adds 5～7 times of its weight by weight in the above-mentioned homogenate, 3. pour the interior centrifugal slagging-off of centrifuge after stirring into and get supernatant, 4. in this supernatant, add ammonium sulfate to 40～70% saturation, 5. left standstill after stirring 10～14 hours, and then the centrifugal precipitate of leaving and taking, 6. this precipitate is dialysed with phosphate buffer dissolving back, liquid is through concentrating in the film, after the drying promptly; Two. extract the dried mussel material, it comprises again: 1. get pure bright mussel meat weigh the back homogenate in high-speed tissue mashing machine, 2. in above-mentioned homogenate, successively add at least three kinds of enzymes, under the condition of 45～55 ℃ of temperature, pH value 2～8.5, stir and hydrolysis 12～18 hours, 3. add 1.5～2.5% activated carbon of above-mentioned homogenate weight and 0.5～1.5% cyclodextrin by weight respectively in the above-mentioned hydrolyzed solution, go into centrifuge centrifuging and taking supernatant behind the decoloration deodorization, 4. this supernatant concentration and drying are promptly made; Three. according in the prescription fixed percentage ratio, get the one and two above-mentioned dry materials that the make back mixing of weighing; Four. the dry material behind the mixing is pulverized, sieved, promptly get powder of the present invention.

An above-mentioned .4. comprises again: add ammonium sulfate to 40～50% saturation in supernatant, pour in the centrifuge centrifugal after the stirring again, leave and take supernatant, with in this supernatant, add such two steps of ammonium sulfate to 60～70% saturation again, and add all need stir behind the ammonium sulfate at every turn and reached centrifugal again collection after the complete equipilibrium in 10～60 minutes.

Two above-mentioned .2. comprise again: 2-1 at first regulates pH value to 2～2.5 of hydrolysate, by weight (down with) add 1～2% pepsin of above-mentioned homogenate weight, stirred 4～6 hours; 2-2. transfer pH value to 6.6～7.5 again, add 1.5～2.5% subtilisin of homogenate weight, stirred 4～6 hours; 2-3, transfer pH value to 7.8～8.5 again, add 2～3% trypsin of homogenate weight, hydrolysis 4～6 hours is warming up to 80～90 ℃ after hydrolysis finishes, and 8～12 minutes, cause enzyme deactivation, promptly get the mussel enzymolysis solution.

Advantage of the present invention is: extensively and always do the refuse processing but the edible fresh scallops limit and abundant the distribution extensively of originating with the big distribution of output, the low also edible bright mussel meat of price is a raw material, also only need bruisher and centrifuge and some common vessels in the leaching process, technology is simpler, small investment, instant effect utilizes these goods to mice transplantability U ₁₄Solid tumor models, liver injury model has carried out observational study respectively due to aged mouse model and the carbon tetrachloride, the result proves that goods of the present invention have prevention and auxiliary treatment to oncosis and hepatopathy and alleviate the function of radiotherapy side effect, and energy defying age and enhancing body antioxidation, improve the human body immunity, the analysis showed that after measured these goods contain abundant organic selenium, 18 seed amino acids that protein and human body are necessary, multivitamin is vitamin E and B especially, also contain proportional suitable zinc simultaneously, ferrum, copper, various trace elements such as manganese, so these goods are nutritious and comprehensive, quality inspection is checked through the Qingdao City, prove that the every quality index of these goods is all up to specification, and

Product quality and performances are all stable, and are because the raw material of these goods itself is two kinds of daily edible seafoods, without any side effects to human body through these goods of toxicological study proof proper amount of edible again.Owing to make full use of scallop edge, turn waste into wealth again, can not only further promote the development of mariculture industry, and can alleviate environmental pollution.

One of embodiment of the invention is: get 34 kilograms on pure Chlamys farreri [Chlamys (Azumapecten) farreri] limit that Rizhao City haze mountain, Shandong Province plant produces, put into high-speed tissue mashing machine's homogenate after adding the phosphate buffer of a small amount of autogamy, in homogenate, add 204 kilograms of phosphate buffers, pour in the centrifuge centrifugal after stirring, leave and take supernatant, and toward wherein adding ammonium sulfate to 45% saturation, stir and pour centrifuge centrifuging and taking supernatant after 10 minutes again into, add ammonium sulfate to 65% saturation again, slowly stir again after 60 minutes and left standstill 12 hours, and then the centrifugal precipitate of leaving and taking, with its dissolving back dialysis, liquid is again through concentrating in the film with phosphate buffer, drying promptly makes scallop edge and extracts dry material 2800 grams.

Get 7 kilograms of the pure meat of Mytilus edulis [Myticlus edulis linne] that red island, Qingdao City plant produces again, put into high-speed tissue mashing machine's homogenate, regulate pH value to 2.0～2.5, add 1: 3000 pepsin of enzyme activity, 105 grams down 50 ℃ of constant temperature, constantly stir hydrolysis 5 hours, transfer pH value to 6.6～7.5 again, the bacillus subtilis neutral proteinase AS1398 that adds enzyme activity 50000IU/g, 140 grams, stir hydrolysis 5 hours, transfer pH value to 7.8～8.5 again, add 1: 25 trypsin of enzyme activity, 175 grams, hydrolysis 5 hours; Be warming up to 85 ℃ and continue 10 minutes, make enzyme deactivation, the mussel enzymolysis solution, go into centrifuge centrifuging and taking supernatant after adding 140 gram activated carbon and 70 gram cyclodextrin decoloration deodorizations in this enzymolysis solution, behind concentrate drying, make mussel again and extract dry material 1507.7 and restrain; Get above-mentioned scallop edge and extract dry material 2800 grams (account for gross weight 65%), mussel pulverizes after extracting dry material 1507.7 grams (account for gross weight 35%) mixing, sieving promptly get powder 4307.7 of the present invention and restrain.

Two of embodiment: one of the processing step of extraction scallop edge dry material and dried mussel material and embodiment are basic identical, only in the processing step that extracts the scallop edge dry material, in homogenate, add 170 kilograms of phosphate buffers, earlier in supernatant, add ammonium sulfate to 40% saturation, stir and in supernatant, add ammonium sulfate to 60% saturation again after centrifugal; Left standstill 10 hours; Make scallop edge and extract dry material 2500 grams; And when extracting the dried mussel material, under 45 ℃ of conditions of constant temperature, pepsin adds 70 grams, and subtilisin adds 105 grams, and Trypsin adds 140 grams, and stirring and hydrolysis all each 4 hours are warming up to 80 ℃ after hydrolysis finishes, continue 8 minutes; Add activated carbon 105 grams and cyclodextrin 35 grams, make mussel and extract dry material 1666.7 grams, get above-mentioned scallop edge and extract dry material 2500 grams (account for gross weight 60%), mussel is extracted dry material 1666.7 grams (account for gross weight 40%) and mixes, pulverizes, sieves to such an extent that make capsule formulation in 4166.7 these goods of gram and 16666 capsules of packing into.

Three of embodiment: the processing step that extracts scallop edge dry material and dried mussel material is also basic identical with one of embodiment, only when extracting the scallop edge dry material, get 40 kilograms of bright pure scallop edges, and in its processing step, in homogenate, add 280 kilograms of phosphate buffers, earlier in supernatant, add ammonium sulfate to 50% saturation, stir and in supernatant, add ammonium sulfate to 70% saturation again after centrifugal, left standstill 14 hours, and made scallop edge and extract dry material 3000 grams; And when extracting the dried mussel material, under 55 ℃ of constant temperature, pepsin adds 140 grams, and subtilisin adds 175 grams, trypsin 210 grams, and stirring and hydrolysis each 6 hours are warming up to 90 ℃ after hydrolysis finishes, continue 12 minutes; Add activated carbon 175 grams and cyclodextrin 105 grams, make mussel and extract dry material 1285.7 grams, get above-mentioned scallop edge and extract dry material 3000 grams (account for gross weight 70%), mussel extracts that dry material 1285.7 grams (account for gross weight 30%) mix, pulverize, sieving makes 4285.7 these goods of gram and be distributed into 8571 pouches.

Change as above-listed embodiment:

1, the raw-material place of production and kind can suitably change, and accomplish treatment in accordance with local conditions;

2, end product can be made various dosage forms;

3, each boundary condition all can suitably be adjusted in the scope of permission.

Claims (4)

1, a kind of selenium product of natural marine organism, it is characterized in that these goods extract dry material by the fresh scallops limit through homogenate, extraction, precipitation, dialysis, concentrated, dry gained scallop edge and account for 60～70% of gross weight by weight, extract dry material with bright mussel meat through homogenate, enzymolysis, centrifugal, concentrated, the dry gained mussel of decoloration deodorization and account for 30～40% of gross weight, the pulverulent mixture that the two mixes mutually, pulverizes, sieves and makes.

2, a kind of preparation method of selenium product of natural marine organism as claimed in claim 1 is characterized in that this method comprises following process steps; One. extract the scallop edge dry material, it comprises again: 1. get and add the small amounts of phosphoric acid salt buffer after weighing in pure fresh scallops limit, i.e. homogenate in high-speed tissue mashing machine, 2. the phosphate buffer that adds 5～7 times of its weight by weight in the above-mentioned homogenate, 3. pour in the centrifuge centrifugal after stirring, slagging-off, get supernatant, 4. in this supernatant, add ammonium sulfate to 40～70% saturation, 5. left standstill after stirring 10～14 hours, and then the centrifugal precipitate of leaving and taking, 6. this precipitate is dialysed with phosphate buffer dissolving back, liquid is through concentrating in the film, after the drying promptly; Two. extract the dried mussel material, it comprises again: 1. get pure bright mussel meat weigh the back homogenate in high-speed tissue mashing machine, 2. in above-mentioned homogenate, successively add at least three kinds of enzymes, under the condition of 45～55 ℃ of temperature, pH value 2～8.5, stir and hydrolysis 12～18 hours, 3. add 1.5～2.5% activated carbon of above-mentioned homogenate weight and 0.5～1.5% cyclodextrin by weight respectively in the above-mentioned hydrolyzed solution, go into centrifuge centrifuging and taking supernatant behind the decoloration deodorization, 4. this supernatant concentration and drying are promptly made; Three. according in the prescription fixed percentage ratio, get the one and two above-mentioned dry materials that the make back mixing of weighing; Four. the dry material behind the mixing is pulverized, sieved, promptly get powder of the present invention.

3, the preparation method of selenium product of natural marine organism according to claim 2, it is characterized in that an above-mentioned .4. comprises again: in supernatant, add ammonium sulfate to 40～50% saturation, pour in the centrifuge centrifugal after the stirring again, leave and take supernatant, with in this supernatant, add such two steps of ammonium sulfate to 60～70% saturation again, and all need stir 10～60 minute after adding ammonium sulfate at every turn, reach centrifugal again collection after the complete equipilibrium.

4, the preparation method of selenium product of natural marine organism according to claim 2, it is characterized in that two above-mentioned .2. comprise again: 2-1 at first regulates pH value to 2～2.5 of hydrolysate, add 1～2% pepsin of homogenate weight by weight, stirred 4～6 hours; 2-2, transfer pH value to 6.6～7.5 again, add 1.5～2.5% subtilisin of homogenate weight, stirred 4～6 hours; 2-3, transfer pH value to 7.8～8.5 again, add 2～3% trypsin of homogenate weight, hydrolysis 4～6 hours is warming up to 80～90 ℃ after hydrolysis finishes, and 8～12 minutes, cause enzyme deactivation, promptly get the mussel enzymolysis solution.