CN1296978A - Process for extracting mucopolysaccharide from pork skin - Google Patents
Process for extracting mucopolysaccharide from pork skin Download PDFInfo
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- CN1296978A CN1296978A CN 99112589 CN99112589A CN1296978A CN 1296978 A CN1296978 A CN 1296978A CN 99112589 CN99112589 CN 99112589 CN 99112589 A CN99112589 A CN 99112589A CN 1296978 A CN1296978 A CN 1296978A
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- mucopolysaccharide
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Abstract
A process for extracting health-care mucopolysaccharide from pork skin includes removing hairs from fresh pork skin of three-month pig, removing fat layer, washing, mincing, immersing in diluted NaOH solution, regulating pH value, adding trypsase, enzymolysis while stirring, separating oil, adding activated carbon, centrifugal separation of supernatant, vacuum concentrating, aseptic drying and pulverizing. Its advantages are simple process, less equipment, high yield, and high content of 13 amino acids.
Description
A kind of production method of extracting mucopolysaccharide (Glycosaminoglycons) from pigskin belongs to the preparing technical field of protective foods, is mainly used in the preparation mucopolysaccharide.
In the prior art, according to appointing the chief editor by Zhang Weijie, 67 experts and scholars collectives of 20 institutes in the whole nations such as Institute of Chemistry, Academia Sinica and institution of higher learning write, " saccharide complex Biochemical Research technology " secretary of press of Zhejiang University publication in 1994 is carried: have only at present from cartilage, extract a small amount of mucopolysaccharide in the flesh tissues such as lung and be used for the method for measuring of GAG total amount, but in leaching process, need to soak with acetone solution, also need add phosphoric acid buffer and small amount of toluene, anorexia usefulness affirmed in the mucopolysaccharide of Ti Quing like this, it is rare to have starting material in addition, go out rate very little, complex process needs to repeat defectives such as enzymolysis with trypsinase and papoid.Only find No. 91107319.1 " from pork skin, extracting hyaluronic manufacture method " application for a patent for invention by retrieval.But the disclosed technical scheme of this patent application is be used for from the dermal tissue of pig extracting the liquid state used as cosmetics additive hyaluronic, and its product extraction yield is low, and the content of 13 seed amino acids is few, can only external application, and anorexia is used.Up to the present, do not see that the report that extracts mucopolysaccharide that can be edible from the reticular layer of the epidermis of pigskin and corium is arranged.
The object of the present invention is to provide a kind of technology fairly simple, required production unit is few, workable, can produce the extraction yield height in enormous quantities, only contain the full-natural nutritive material in the product, do not add any chemical reagent, the content of 13 seed amino acids is all higher, can be edible, abundant raw materials is easy to get, the production method of extracting mucopolysaccharide from pigskin of environmentally safe in the production process.
Characteristic and pigskin weave construction characteristics according to mucopolysaccharide, at existing questions in the prior art, basic design of the present invention is: with three monthly ages unhairing and the fresh pig skin that scrapes off the dermal fat layer is raw material, put into dilute NaOH solution after it clean is rubbed and soak, then through transferring pH value, add trypsin digestion, separate grease, adding that activated carbon stirs, centrifugal supernatant liquor, vacuum concentration and the processing steps such as aseptic drying and pulverizing of separating out promptly make oyster white powdery mucopolysaccharide.
The production method of from pigskin, extracting mucopolysaccharide of the present invention, comprise unhairing, scrape off the lipid layer of corium, clean and with processing steps such as mincer rubbings, it is characterized in that this method be with three the monthly age live pig fresh pig skin be raw material and except that above-mentioned processing step, also comprise: (1) is by weight in by the skin grain that rubbed: concentration is that the ratio of 0.5% NaOH solution=1: 3~5 is soaked the skin grain 10~14 hours, (2) transfer PH to 7~9 backs to add 1: 250 trypsinase 0.8~1.2 gram by per kilogram skin grain, enzymolysis is removed grease through the liquid distributing device separation again after 8~14 hours and is made no fat filtrate under 35~39 ℃ of temperature condition, (3) by weight in no fat filtrate: add activated carbon in the no fat filtrate that the ratio of activated carbon=1: 0.5~1.5% makes toward above-mentioned (2) and stirred 1.5~2.5 hours, (4) separate with 5000~7000 rev/mins rotating speed with whizzer and make supernatant liquor, (5) this supernatant liquor is carried out vacuum concentration and aseptic drying makes yellowish block, pulverize through 80~120 orders again and promptly get this patent product mucopolysaccharide powder.
Advantage of the present invention is: technology is fairly simple, required production unit is few, workable, less investment, instant effect, can produce in enormous quantities, the extraction yield height, only contain the full-natural nutritive material in the product, water-soluble entirely, show that through check and analysis results of scientific research institution such as centralaies of the Institute of Oceanology of the Chinese Academy of Sciences 13 seed amino acid content such as aspartic acid are all higher, wherein among glycine, L-glutamic acid, aspartic acid, the every 100mg of proline(Pro) respectively up to 27.01,16.70,7.10 and 4.82mg; All considerably beyond No. 91107319.1 disclosed homogeneous datas of patent application, the more important thing is owing to do not add the material that any anorexia is used in the leaching process, thereby this product can be directly edible for people as senior tonic, abundant raw materials is easy to get, environmentally safe in the production process, production cost is much lower more than extracting from tissues such as umbilical cord or cockscomb.
As one of embodiments of the invention be: get the onal sheet that is cut into earlier after 10 kilograms unhairing has gone three monthly age fresh pig skins of dermal fat layer to clean about 2 * 4cm, be broken into the skin grain of about 0.5cm again with mincer, add 40 kilograms of concentration and be 0.5% NaOH solution, the PH that soaks under the room temperature and often stir after 12 hours skin grain that will be in the pasty state is transferred to 8~8.6, then toward trypsinase 10 grams that wherein drop into 1: 250, enzymolysis under 37 ℃ of conditions of temperature, stir about 12 hours, earlier use 80 purpose silk cover filterings again behind the residue that is not finished by enzymolysis with the removal of 40 purpose silk cover filterings, filtrate is gone into transparent liquid distributing device, left standstill 15 minutes, the grease that will be positioned at the upper strata after the layering is separated, make no oily filtrate, stirred 2 hours after adding 1% heavy granular activated charcoal of this liquid, centrifugal with 6000 rev/mins of whizzers then, get clarifying supernatant liquor, through vacuum concentration, get little yellow colloid, carry out the vacuum-drying slabbing with the sterilizing-drying case again, at last this flap is ground into 100 purpose oyster white fine powders, makes the complete water-soluble edible mucopolysaccharide powder of 500 grams.
Change as present embodiment:
1, soak skin grain usefulness NaOH solution concentration and all can suitably change in tolerance band with the ratio of skin grain and soak time etc., the two can also volume ratio calculate;
2, per kilogram skin grain adds tryptic quantity, hydrolysis temperature and time and all can suitably adjust in the scope of permission.
Claims (1)
1, a kind of production method of from pigskin, extracting mucopolysaccharide, comprise unhairing, scrape off the lipid layer of corium, clean and with processing steps such as mincer rubbings, it is characterized in that this method is that fresh pig skin with three monthly ages is raw material and also comprises except that above-mentioned processing step: (1) is by weight in the skin grain that is rubbed: concentration is that the ratio of 0.5% NaOH solution=1: 3~5 is soaked the skin grain 10~14 hours, (2) transfer PH to 7~9 backs to add 1: 250 trypsinase 0.8~1.2 gram by per kilogram skin grain, enzymolysis is removed grease through the liquid distributing device separation again after 8~14 hours and is made no fat filtrate under 35~39 ℃ of temperature condition, (3) by weight in no fat filtrate: add activated carbon in the no fat filtrate that the ratio of activated carbon=1: 0.5~1.5% makes toward above-mentioned (2) and stirred 1.5~2.5 hours, (4) separate with 5000~7000 rev/mins rotating speed with whizzer and make supernatant liquor, (5) this supernatant liquor is carried out vacuum concentration and aseptic drying makes yellowish block, pulverize through 80~120 orders again and promptly get this patent product mucopolysaccharide powder.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN 99112589 CN1296978A (en) | 1999-11-23 | 1999-11-23 | Process for extracting mucopolysaccharide from pork skin |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN 99112589 CN1296978A (en) | 1999-11-23 | 1999-11-23 | Process for extracting mucopolysaccharide from pork skin |
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CN1296978A true CN1296978A (en) | 2001-05-30 |
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CN 99112589 Pending CN1296978A (en) | 1999-11-23 | 1999-11-23 | Process for extracting mucopolysaccharide from pork skin |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105107815A (en) * | 2015-06-25 | 2015-12-02 | 河南农业大学 | Method for treating illness-dead livestock and poultry by combining chemical method with biological method |
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1999
- 1999-11-23 CN CN 99112589 patent/CN1296978A/en active Pending
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105107815A (en) * | 2015-06-25 | 2015-12-02 | 河南农业大学 | Method for treating illness-dead livestock and poultry by combining chemical method with biological method |
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PB01 | Publication | ||
C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
WD01 | Invention patent application deemed withdrawn after publication |