CN1296853A - Process for preparing medical pig skin - Google Patents
Process for preparing medical pig skin Download PDFInfo
- Publication number
- CN1296853A CN1296853A CN 99123943 CN99123943A CN1296853A CN 1296853 A CN1296853 A CN 1296853A CN 99123943 CN99123943 CN 99123943 CN 99123943 A CN99123943 A CN 99123943A CN 1296853 A CN1296853 A CN 1296853A
- Authority
- CN
- China
- Prior art keywords
- skin
- preparation
- pig
- sus domestica
- corii sus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000004519 manufacturing process Methods 0.000 title abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 210000003491 skin Anatomy 0.000 claims description 56
- 241000282898 Sus scrofa Species 0.000 claims description 30
- 238000002360 preparation method Methods 0.000 claims description 25
- 241000282894 Sus scrofa domesticus Species 0.000 claims description 21
- 210000002615 epidermis Anatomy 0.000 claims description 5
- 238000001291 vacuum drying Methods 0.000 claims description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 3
- 210000004209 hair Anatomy 0.000 claims description 3
- 238000004080 punching Methods 0.000 claims description 3
- 238000005096 rolling process Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 238000003860 storage Methods 0.000 abstract description 4
- 238000005520 cutting process Methods 0.000 abstract description 3
- 230000035699 permeability Effects 0.000 abstract description 2
- 239000002504 physiological saline solution Substances 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- 208000027418 Wounds and injury Diseases 0.000 description 17
- 150000001875 compounds Chemical class 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 230000035515 penetration Effects 0.000 description 3
- 229910052709 silver Inorganic materials 0.000 description 3
- 239000004332 silver Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010072170 Skin wound Diseases 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 238000005485 electric heating Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 210000000416 exudates and transudate Anatomy 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 210000003780 hair follicle Anatomy 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 238000010562 histological examination Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- OGJPXUAPXNRGGI-UHFFFAOYSA-N norfloxacin Chemical compound C1=C2N(CC)C=C(C(O)=O)C(=O)C2=CC(F)=C1N1CCNCC1 OGJPXUAPXNRGGI-UHFFFAOYSA-N 0.000 description 1
- 229960001180 norfloxacin Drugs 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000013138 pruning Methods 0.000 description 1
- 229910001961 silver nitrate Inorganic materials 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
Landscapes
- Medicinal Preparation (AREA)
Abstract
A process for preparing medical pig skin includes killing pig, stripping skin, removing fat, getting surficial pig skin of 0.4-0.8 mm in thickness, cutting to needed sizes, washing with physiological saline, putting it in constant-temp electric vacuum drier for 24 hr to make its water content be 5%, radiating with Co 60, and storage in sealed state. Its advantages are good elasticity, adhesion and water permeability. It can be used to treat burn.
Description
The present invention puts it briefly and relates to the preparation method of a kind of surgery with biological dressing, specifically relates to a kind of preparation method for the treatment of the Corii Sus domestica of burn usefulness.
Medical pig skin is a kind of good burn wound's dressing, and it is to protection burn wound, and minimizing is oozed out, protects from infection, alleviated patient's pain and plays an important role.At present, the medical pig skin of the treatment burn usefulness of people's employing has fresh porcine skin, radiated pig skin, cold preservation Corii Sus domestica, glutaric pigskin.These Corii Sus domesticas mostly need low tempertaure storage except that fresh porcine skin, period of storage is short, and transportation is inconvenient, and cost is higher.
The object of the present invention is to provide a kind of manufacturing easy, with low cost, be easy to store and the preparation method of transportation, medical pig skin that period of storage is long, easy to use.
The preparation method of medical pig skin of the present invention comprises the following steps: successively
1, gets the white pig of 10-80 kilogram weight, live and kill, shave hair, clean peeling, degrease;
2, get the Corii Sus domestica that thickness is 0.4～0.8 millimeter band epidermis with the bark fetching drum;
3, the Corii Sus domestica that takes off is cut into the suitable skin graft of length and width;
4, clean up with normal saline;
5, put into electric vacuum drying oven, at 38～44 ℃, 1 * 10
-5Deposit about 24 hours under the vacuum condition of the holder left and right sides, its water content is reached about 5%;
6, use
60C
oThe radiometer irradiation sterilization;
7, sealing is stored.
The preparation method of medical pig skin of the present invention preferably adds between processing step 4 and 5 with rolling the operation of skin machine with the Corii Sus domestica punching.
The white pig that the preparation method of medical pig skin of the present invention is used heavily is preferably the 50-60 kilogram.
The preparation method of medical pig skin of the present invention is preferably 0.6 millimeter with the Corii Sus domestica thickness of the band epidermis that the bark fetching drum takes off.
The preparation method of medical pig skin of the present invention, preferably will be cut into the Corii Sus domestica that the bark fetching drum takes off long 20-30 centimetre, wide 10-15 centimetre skin graft.
The preparation method of medical pig skin of the present invention is used
60C
oThe time of radiometer irradiation is preferably 15-20 minute, and exposure dose is 125kgy.
The preparation method of medical pig skin of the present invention, wherein the temperature in the constant temperature vacuum drying oven is best 40 ℃.
The preparation method of medical pig skin of the present invention, its employed equipment all is commercially available conventional equipments, make easyly, with low cost, can be at normal temperatures preserve naturally with its medical pig skin that produces, need not to use household freezer, so it stores convenient transportation, and is easy to use, the dry medical pig skin of being produced is compared with fresh porcine skin through test, its tearing strength, ductility, wound surface bonding force, water permeability do not have significance, and clinical application effect is similar to the Corii Sus domestica that adopts at present.
Other details of the preparation method of medical pig skin of the present invention and characteristics can be cheer and bright by reading the embodiment hereinafter encyclopaedize:
1, the white pig of getting the 50-60 kilogram lives extremely, shaves hair, cleaning, peeling, the fat of pruning.
2, get the Corii Sus domestica that thickness is 0.6 millimeter band epidermis with electronic bark fetching drum, and it is cut wide 10-15 centimetre, long 20-30 centimetre skin graft;
3, clean up with normal saline;
4, with the smooth electric heating constant temperature vacuum tank of putting into of skin graft, for example put into commercially available health and happiness board ZDK-88A type electric vacuum drying oven, the temperature inside the box is transferred to 40 ℃, and start vacuum pump and will be evacuated in the case, make vacuum reach 1 * 10
-5Holder kept 24 hours, and the skin graft water content is reached about 5%;
5, with rolling the punching of skin machine;
6, use
60C
oRadiometer irradiation 15 minutes, irradiation dose are 125kgy;
7, putting into compound plastic bag seals up for safekeeping.
Dried pig-skin with the inventive method preparation needs to make its recovery in 10 hours with compound recipe silver solution soaking before use, is fixed on the wound surface with suture then, and uses the gauze pressure dressing, and second day after operation exposes.Compound recipe silver solution main component used herein is the norfloxacin of 0.5% silver nitrate and 2%.
Medical dried pig-skin with the inventive method preparation is carried out biological property measure, the result is as follows:
Elasticity measurement: ductility and tearing strength with Corii Sus domestica are tested restorative dried pig-skin of thick 0.6～0.8mm and fresh porcine skin (matched group) as elastic index, measure two kinds of skin grafts each 15 sample tearing strengths and ductility.The result: dried pig-skin and fresh porcine skin comparison tearing strength and ductility difference do not have significance (P＞0.05).
See Table 1
The tearing strength of two types of Corii Sus domesticas of table 1 and ductility (x ± s)
Skin graft kind sample number tearing strength is extended situation
Ductility (mm) is extended percent (%)
Fresh porcine skin 15 76.4 ± 16.4 15.1 ± 3.8 66.54 ± 19.0
Dried pig-skin 15 86.4 ± 14.3 12.67 ± 2.3 63.46 ± 10.4
The wound surface bonding force is measured: laboratory animal is selected healthy rabbits for use, respectively excise holostrome skin 4cm * 4cm in the rabbit both sides and form symmetrical wound surface, to test skin graft again and be cut into 1cm * 2cm, the dried pig-skin after the recovery, alternate the sticking on the rabbit wound surface of fresh porcine skin (matched group) are measured bonding force.See Table 2.The result: dried pig-skin does not have significance (P＞0.05) with new Corii Sus domestica comparison wound surface bonding force difference.
Table 2 72h dried pig-skin and fresh porcine skin wound surface bonding force measurement result (x ± s)
Integumentary pattern sample number wound surface bonding force (g/cm
2)
Fresh porcine skin 15 57.71 ± 25.62
Dried pig-skin 15 63.24 ± 11.49
Water penetration is measured: measure skin graft and be dried pig-skin, fresh porcine skin (matched group) after restoring.The result: the permeable amount comparing difference of dried pig-skin and fresh porcine skin does not have significance (P＞0.05), sees Table 3.
Two kinds of skin graft water penetrations of table 3 measurement result (x ± s)
Permeable amount (the g/m of skin graft kind sample number
2)
Fresh porcine skin 15 157.06 ± 44.57
Dried pig-skin 15 160.14 ± 43.25
Histological examination: 15 samples of dried pig-skin, the row light microscopy checking that use preparation.From observation by light microscope to: the dried pig-skin epidermal structure is complete, and the part epithelial cell is refused to dye, and intradermal collagen fiber structure is also more normal, and accessory structures such as hair follicle are complete, and cellularity is normal.
Be the clinical application effect of the Corii Sus domestica determining to produce with the inventive method, the inventor has selected 45 routine patients as object of study (wherein male's 34 examples, women's 11 examples), patient age 1～68 years old, average 34.5 years old, burn surface area 5%-92%, average 48.54% ± 6.37%.The third degree burn area is 5%-24%.It is 1-2 days 7 examples, 3-5 days 29 examples, 9 examples more than 7 days blanking time that dried pig-skin is used in the operation of being admitted to hospital from burning to.
Application process: with the dried pig-skin of preparation
60C
oRadiation treatment, with compound recipe silver solution soaking 10h, it is standby to roll the skin machine back that burrows, and cuts crust and cuts crust by the degree of depth of burn wound with preceding.Cut crust group case dried pig-skin and use 34 examples, cut crust group patient 11 examples and plant behind the body skin and cover dried pig-skin.Cutting crust group area is 45.15% ± 4.15%, and cutting crust group area is 16.24% ± 5.17%.
Observation on Clinical Application all becomes brown-black to the dried pig-skin color in faint yellow 1 week of beginning, quality begins softness, and the back hardening of 2 weeks separates with wound surface at leisure.
Cut crust group 34 example and use dried pig-skins, the wherein disposable healing of 30 routine patient's wound surface, with the average adhesion time of wound surface be 12.14 ± 5.46 days.4 routine patients are arranged because of the dissolving of dried pig-skin pressurized Corii Sus domestica, wound surface is not healed.
Cut crust group 11 examples and use dried pig-skin, it is less that the crust wound exudate is cut in the dried pig-skin covering, begins to occur being partly dissolved at 2 all left and right sides dried pig-skins, and the part hardening separates self-skin transplant and survives, expands, covers and cut the crust wound surface with wound surface.
Be not difficult to find out thus, the Corii Sus domestica of producing with the preparation method of medical pig skin of the present invention is compared difference in elasticity, adhesiveness and water penetration with fresh porcine skin, and there are no significant, clinically replace other medical pig skins respond well as biological dressing, and its manufacturing is easy, cheap, store convenient transportation.
Claims (7)
1, a kind of preparation method of medical pig skin comprises the following steps: successively
(1) gets the white pig of 10-80 kilogram weight, live and kill, shave hair, clean peeling, degrease;
(2) get the Corii Sus domestica that thickness is 0.4～0.8 millimeter band epidermis with the bark fetching drum;
(3) Corii Sus domestica that takes off is cut into the suitable skin graft of length and width;
(4) clean up with normal saline;
(5) put into electric vacuum drying oven, at 38～44 ℃, 1 * 10
-5Deposit about 24 hours under the vacuum condition of the holder left and right sides, its water content is reached about 5%;
(6) use
60C
oThe radiometer irradiation sterilization;
(7) sealing is stored.
2,, it is characterized in that between processing step 4 and 5, adding with rolling the operation of skin machine with the Corii Sus domestica punching according to the preparation method of the described medical pig skin of claim 1.
3,, it is characterized in that the white pig weight of using is the 50-60 kilogram according to the preparation method of claim 1 or 2 described medical pig skins.
4,, it is characterized in that the Corii Sus domestica thickness of the band epidermis that takes off with the bark fetching drum is 0.6 millimeter according to the preparation method of the described medical pig skin of claim 3.
5,, it is characterized in that the Corii Sus domestica that will take off with the bark fetching drum is cut into long 20-30 centimetre, wide 10-15 centimetre skin graft according to the preparation method of the described medical pig skin of claim 4.
6,, it is characterized in that using according to the preparation method of the described medical pig skin of claim 5
60C
oThe time of radiometer irradiation is 15-20 minute, and exposure dose is 125kgy.
7,, it is characterized in that the temperature in the constant temperature vacuum drying oven is 40 ℃ according to the preparation method of the described medical pig skin of claim 6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99123943 CN1296853A (en) | 1999-11-18 | 1999-11-18 | Process for preparing medical pig skin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99123943 CN1296853A (en) | 1999-11-18 | 1999-11-18 | Process for preparing medical pig skin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1296853A true CN1296853A (en) | 2001-05-30 |
Family
ID=5283067
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 99123943 Pending CN1296853A (en) | 1999-11-18 | 1999-11-18 | Process for preparing medical pig skin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1296853A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100400111C (en) * | 2006-10-27 | 2008-07-09 | 邯郸钢铁股份有限公司 | Preparation method of pig or bovine bladder membranous biological dressing for treating burns |
| CN100415309C (en) * | 2003-07-03 | 2008-09-03 | 中国人民解放军第三○四医院 | Application of selective decellularized pig skin in the preparation of human skin substitute and its preparation method |
| CN100427154C (en) * | 2005-01-27 | 2008-10-22 | 天津市托福医用原子能科技有限公司 | SD-Zn irradiated biological skin and its preparation method |
-
1999
- 1999-11-18 CN CN 99123943 patent/CN1296853A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100415309C (en) * | 2003-07-03 | 2008-09-03 | 中国人民解放军第三○四医院 | Application of selective decellularized pig skin in the preparation of human skin substitute and its preparation method |
| CN100427154C (en) * | 2005-01-27 | 2008-10-22 | 天津市托福医用原子能科技有限公司 | SD-Zn irradiated biological skin and its preparation method |
| CN100400111C (en) * | 2006-10-27 | 2008-07-09 | 邯郸钢铁股份有限公司 | Preparation method of pig or bovine bladder membranous biological dressing for treating burns |
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|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |