CN1290704A - Synthesis of 1-hydroxy, 6 or 5-acetyl monosaccharide benzoylate - Google Patents
Synthesis of 1-hydroxy, 6 or 5-acetyl monosaccharide benzoylate Download PDFInfo
- Publication number
- CN1290704A CN1290704A CN 99119755 CN99119755A CN1290704A CN 1290704 A CN1290704 A CN 1290704A CN 99119755 CN99119755 CN 99119755 CN 99119755 A CN99119755 A CN 99119755A CN 1290704 A CN1290704 A CN 1290704A
- Authority
- CN
- China
- Prior art keywords
- benzoyl
- ethanoyl
- hydroxyl
- hexose
- trityl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
Hexasaccharide with triphenylmethyl radical in the sixth place and benzoyl radicals in other places or pentasaccharide with triphenylmethyl radical in the fifth place and benzoyl radicals in other places in acetylated in an acetic anhydride-sulfuric acid or an acetic anhydride-acetic acid-sulfuric acid system into hexasaccharide with acetyl radical in the first and sixth places and benzyl radical in other places or pentasaccharide with acetyl radical in the first and fifth places and benzyl radical in other places, which is made to react with equimolar ammonium carbonate in dimethyl formamide solvent to produce 1-hydroxy 6-actyl hexasaccharide with benzoyl radical in other places or 1-hydroxy 5-actyl pentasaccharide with benzoyl radical in other places of the present invention.
Description
The invention belongs to the preparing technical field of bioactive oligosaccharides; particularly relate to and to be used for synthetic complex oligosaccharide; the important intermediate of using as plant ego defense system activating agent and lentinan core oligosaccharide fragment-1 is that hydroxyl, 6 are ethanoyl, other is that the hexose of benzoyl or 1 are that hydroxyl, 5 are ethanoyl, other is the synthetic method of the five-carbon sugar of benzoyl.
Oligosaccharides, polysaccharide and glycoconjugate (glycoprotein, glycolipid) is important information substance in the organism, participates in the contact process of all cells, the communication of the oligosaccharides of cell surface between cell, identification and interaction, the embryo is taken place, and shifts, in the signal transmission, cell movement with stick, and the interaction aspect of cause of disease and host cell plays an important role.Up-to-date studies show that, oligosaccharides not only with they conjugate in action, a lot of oligosaccharides itself just have the important physical function, the oligosaccharides that has can excite the immunity system of plant, the oligosaccharides that has can be induced the nitrogen fixation of root nodule bacterium; The oligosaccharides that has can combine with the glycoprotein on the microorganism of invading and stop the invasion and attack of these microorganisms to the human normal cell, some oligosaccharides then have the function of heparin (haparin), the medicine of the anti-curing cancers that blood group decision family oligosaccharides gets a good chance of especially.Oligosaccharides is in agricultural, and the pharmaceutical sector aspect has wide practical use.And oligosaccharides and polysaccharide are removed disease directly as drug use, improve health, and will cause the renewal of disease preventing and treating idea, also are that of life science makes progress greatly.Emerging " sugared engineering " (based on drug development of carbohydrate) is in flourish ground zero, according to " biotechnology news " (Biotech News) 1995,14 (4), 7 the report, the market share of carbohydrate medicine will from 1993 13% increase to 2000 24%.
Can adopt several different methods to the synthetic of oligosaccharides, but the first-elected Schmidt method of most important method.This just need at first prepare 1 for hydroxyl, other have various protecting groups sugar.Preparing 1 is hydroxyl, other existing many effective meanss of monose for Bian Ji protection or ethanoyl protection.1 of existing preparation for hydroxyl, 6 for ethanoyl, other for the hexose of benzoyl or 1 for hydroxyl, 5 for ethanoyl, other is that method of the five-carbon sugar of benzoyl then exists yield low, separation difficulty, process complicated problems.This just needs a kind of yield height of development, and easy to operate, raw material is cheap, can prepare the method for this compounds in a large number.
The objective of the invention is to through 1; 6 is ethanoyl, other is the hexose of benzoyl or 1; 5 for ethanoyl, other is intermediate for the five-carbon sugar of benzoyl; provide a kind of step simple, 1 of time saving and energy saving preparation be hydroxyl, 6 for ethanoyl, other for the hexose of benzoyl or 1 for hydroxyl, 5 for ethanoyl, other is synthetic method of the five-carbon sugar of benzoyl.
The object of the present invention is achieved like this: with 6 be trityl, other is that the hexose of benzoyl or 5 are trityl, other is the five-carbon sugar of benzoyl; make it almost be converted into 1 quantitatively through acetolysls; 6 is ethanoyl, other is for the hexose of benzoyl or 1,5 are ethanoyl, other is the five-carbon sugar of benzoyl.Owing to remove the existing multiple high-efficiency method of 1 ethanoyl, therefore can obtain with high yield, easily 1 for hydroxyl, 6 for ethanoyl, other for the hexose of benzoyl or 1 for hydroxyl, 5 are ethanoyl, other is the five-carbon sugar of benzoyl.Through 1,6 be ethanoyl, other for the hexose of benzoyl or 1,5 for ethanoyl, other is that intermediate is a key of the present invention for the five-carbon sugar of benzoyl.
Synthetic method of the present invention is: 6 for trityl, other is for the hexose of benzoyl or 5 are trityl, other is the five-carbon sugar of benzoyl; in acetic anhydride-sulfuric acid or acetic anhydride-acetic acid-vitriolic system, reaction is after 2-20 hour under stirring at room.With reaction solution with organic solvent (as methylene dichloride; ethyl acetate) after the dilution; add entry; aqueous phase discarded after the layering; organic phase is washed till alkalescence with saturated sodium bicarbonate solution, washes with water to neutrality again, drains almost under vacuum and obtains 1 quantitatively; 6 is ethanoyl, other is for the hexose of benzoyl or 1,5 are ethanoyl, other is the five-carbon sugar of benzoyl.It is dissolved in the dimethyl formamide, adds the reaction under stirring under 30 ℃ of equimolar volatile salt and after organic solvent (as methylene dichloride, ethyl acetate) dilution, add water washing, aqueous phase discarded after the layering after 20 hours.Organic phase is drained under vacuum; gained crude product silica gel column chromatography purifying, 1 of obtaining preparing with the inventive method is that hydroxyl, 6 are ethanoyl, other is that the hexose of benzoyl or 1 are that hydroxyl, 5 are ethanoyl, other is the five-carbon sugar of benzoyl.
Described 6 for trityl, other for the hexose of benzoyl be 6 be trityl, other is the glucose of benzoyl; 6 is trityl, other is the semi-lactosi of benzoyl, and 6 is trityl, other is the seminose of benzoyl.
Described 5 for trityl, other for the five-carbon sugar of benzoyl be 5 be trityl, other is the wood sugar of benzoyl; 5 is trityl, other is the pectinose of benzoyl, and 5 is trityl, other is the lyxose of benzoyl.
Described 1,6 for ethanoyl, other for the hexose of benzoyl be 1,6 be ethanoyl, other is the glucose of benzoyl; 1; 6 is ethanoyl, other is the semi-lactosi of benzoyl, and 1,6 is ethanoyl, other is the seminose of benzoyl.
Described 1,5 for ethanoyl, other for the five-carbon sugar of benzoyl be 1,5 be ethanoyl, other is the wood sugar of benzoyl; 1; 5 is ethanoyl, other is the pectinose of benzoyl, and 1,5 is ethanoyl, other is the lyxose of benzoyl.
Described 1 for hydroxyl, 6 for ethanoyl, other for the hexose of benzoyl be 1 for hydroxyl, 6 are ethanoyl, other is the glucose of benzoyl; 1 is hydroxyl, 6 for ethanoyl, other be the semi-lactosi of benzoyl, and 1 is that hydroxyl, 6 are ethanoyl, other is the seminose of benzoyl.
Described 1 for hydroxyl, 5 for ethanoyl, other for the five-carbon sugar of benzoyl be 1 for hydroxyl, 5 are ethanoyl, other is the wood sugar of benzoyl; 1 is hydroxyl, 5 for ethanoyl, other be the pectinose of benzoyl, and 1 is that hydroxyl, 5 are ethanoyl, other is the lyxose of benzoyl.
With following is example
The a-methylene dichloride; acetic anhydride/acetic acid/sulfuric acid; room temperature; 2-20 hour b-dimethyl formamide; volatile salt; 30 ℃, 20 hours 1---6 positions are trityl, other (being glucose among the figure) sugared 2---1 for benzoyl, and 6 is that hydroxyl, 6 are ethanoyl, other (being glucose among the figure) sugar for benzoyl for ethanoyl, other (being glucose among the figure) sugared 3---1 position for benzoyl.
With glucose, semi-lactosi, seminose, wood sugar, pectinose, lyxose are raw material, by their 6 or 5 of the method for (Methodsin Carbohydrate Chemistry II) preparation be trityl, other is the sugar of benzoyl.
The present invention can prepare the synthetic used monose intermediate of complex oligosaccharide, and these intermediates are made Schmidt reagent and be can be used for that the synthetic of bioactive oligosaccharides arranged, and these oligosaccharides can be used for the pharmaceutical activity experiment.
Below in conjunction with embodiment the present invention is described in detail.Embodiment 1:1 position is that hydroxyl, 6 are ethanoyl, other preparation for the glucose of benzoyl
6 is trityl, other is the glucose of benzoyl (1; 30 grams; 35.80 mmole) be dissolved in 50 milliliters of methylene dichloride; in this solution, add 30 milliliters of acetic anhydride; 30 milliliters of acetic acid and 15 milliliters of sulfuric acid; reaction added 200 milliliters of methylene dichloride after 2 hours under the room temperature, under agitation adds less water then excessive acetic anhydride is decomposed.Add a large amount of water washings, aqueous phase discarded after the layering is gone the pickling in the organic phase with saturated sodium hydrogen carbonate solution then.Organic phase with anhydrous sodium sulfate drying after, under vacuum, drain, obtain product 2, its purity can be directly used in next step reaction greater than 90%.Products therefrom 2 is dissolved in 150 milliliters of dimethyl formamide solutions, the volatile salt that adds 15 gram porphyrizes is after reacting 20 hours under the 30 degree temperature, add 200 milliliters of methylene dichloride, wash solution with water, aqueous phase discarded, organic phase is drained under vacuum, crude product is refining with silica gel column chromatography, as leacheate drip washing, collects respective components with ethyl acetate/petroleum ether (1/4), obtain the monose compound 3 (16.0 gram) that the inventive method is prepared, overall yield 84%
Embodiment 2:1 position is that hydroxyl, 6 are ethanoyl, other preparation for the semi-lactosi of benzoyl
6 is trityl, other is the semi-lactosi of benzoyl (4; 20 grams; 23.86 mmole) be dissolved in 40 milliliters of methylene dichloride; in this solution, add 20 milliliters of acetic anhydride and 10 milliliters of sulfuric acid; reaction is after 6 hours under the room temperature; add 150 milliliters of methylene dichloride, under agitation add less water then excessive acetic anhydride is decomposed.Add a large amount of water washings, aqueous phase discarded after the layering is gone the pickling in the organic phase with saturated sodium hydrogen carbonate solution then.Organic phase with anhydrous sodium sulfate drying after, under vacuum, drain, obtain product 5, its purity can be directly used in next step reaction greater than 90%.Products therefrom 5 is dissolved in 120 milliliters of dimethyl formamide solutions, the volatile salt that adds 10 gram porphyrizes is after reacting 20 hours under the 30 degree temperature, add 150 milliliters of methylene dichloride, wash solution with water, aqueous phase discarded, organic phase is drained under vacuum, crude product is refining with silica gel column chromatography, as leacheate drip washing, collects respective components with ethyl acetate/petroleum ether (1/4), obtain the monose compound 6 (10.7 gram) that the inventive method is prepared, overall yield 84%
Embodiment 3:1 position is that hydroxyl, 6 are ethanoyl, other preparation for the seminose of benzoyl
6 is trityl, other is the seminose of benzoyl (7; 38 grams; 45.35 mmole) be dissolved in 68 milliliters of methylene dichloride; in this solution, add 35 milliliters of acetic anhydride; 35 milliliters of acetic acid and 17 milliliters of sulfuric acid; reaction added 200 milliliters of methylene dichloride after 12 hours under the room temperature, under agitation adds less water then excessive acetic anhydride is decomposed.Add a large amount of water washings, aqueous phase discarded after the layering is gone the pickling in the organic phase with saturated sodium hydrogen carbonate solution then.Organic phase with anhydrous sodium sulfate drying after, under vacuum, drain, obtain product 8, its purity can be directly used in next step reaction greater than 95%.Products therefrom 8 is dissolved in 160 milliliters of dimethyl formamide solutions, the volatile salt that adds 16 gram porphyrizes is after reacting 20 hours under the 30 degree temperature, add 200 milliliters of methylene dichloride, wash solution with water, aqueous phase discarded, organic phase is drained under vacuum, crude product is refining with silica gel column chromatography, as leacheate drip washing, collects respective components with ethyl acetate/petroleum ether (1/4), obtain the monose compound 9 (21.6 gram) that the inventive method is prepared, overall yield 89%
Embodiment 4:1 position is that hydroxyl, 5 are ethanoyl, other preparation for the pectinose of benzoyl
5 is trityl, other is the pectinose of benzoyl (10; 15 grams; 21.3 mmole) be dissolved in 35 milliliters of methylene dichloride; in this solution, add 17 milliliters of acetic anhydride; 17 milliliters of acetic acid and 8 milliliters of sulfuric acid; reaction added 130 milliliters of methylene dichloride after 15 hours under the room temperature, under agitation adds less water then excessive acetic anhydride is decomposed.Add a large amount of water washings, aqueous phase discarded after the layering is gone the pickling in the organic phase with saturated sodium hydrogen carbonate solution then.Organic phase with anhydrous sodium sulfate drying after, under vacuum, drain, obtain product 11, its purity can be directly used in next step reaction greater than 90%.Products therefrom 11 is dissolved in 100 milliliters of dimethyl formamide solutions, the volatile salt that adds 7 gram porphyrizes is after reacting 20 hours under the 30 degree temperature, add 130 milliliters of methylene dichloride, wash solution with water, aqueous phase discarded, organic phase is drained in vacuum, crude product is refining with silica gel column chromatography, as leacheate drip washing, collects respective components with ethyl acetate/petroleum ether (1/4), obtain the monose compound 12 (6.3 gram) that the inventive method is prepared, overall yield 74%
Embodiment 5:1 position is that hydroxyl, 5 are ethanoyl, other preparation for the wood sugar of benzoyl
5 is trityl, other is the wood sugar of benzoyl (13; 16 grams; 22.72 mmole) be dissolved in 30 milliliters of methylene dichloride; in this solution, add 16 milliliters of acetic anhydride and 8 milliliters of sulfuric acid; reaction is after 20 hours under the room temperature; add 100 milliliters of methylene dichloride, under agitation add less water then excessive acetic anhydride is decomposed.Add a large amount of water washings, aqueous phase discarded after the layering is gone the pickling in the organic phase with saturated sodium hydrogen carbonate solution then.Organic phase with anhydrous sodium sulfate drying after, under vacuum, drain, obtain product 14, its purity can be directly used in next step reaction greater than 90%.Products therefrom 14 is dissolved in 80 milliliters of dimethyl formamide solutions, the volatile salt that adds 8 gram porphyrizes is after reacting 20 hours under the 30 degree temperature, add 100 milliliters of methylene dichloride, wash solution with water, aqueous phase discarded, organic phase is drained in vacuum, crude product is refining with silica gel column chromatography, as leacheate drip washing, collects respective components with ethyl acetate/petroleum ether (1/4), obtain the monose compound 15 (6.4 gram) that the inventive method is prepared, overall yield 70%
Claims (4)
1. one kind with 1; 6 is ethanoyl, other is the hexose of benzoyl or 1; 5 for ethanoyl, other for the five-carbon sugar of benzoyl be 1 of intermediate preparation for hydroxyl, 6 for ethanoyl, other for the hexose of benzoyl or 1 for hydroxyl, 5 are ethanoyl, other is synthetic method of the five-carbon sugar of benzoyl, it is characterized in that:
(1) 6 be trityl, other for the hexose of benzoyl or 5 for trityl, other is the five-carbon sugar of benzoyl; in acetic anhydride-sulfuric acid or acetic anhydride-acetic acid-vitriolic system acetolysls 2-20 hour; with ordinary method processing reaction liquid; almost obtain 1 quantitatively; 6 is ethanoyl, other is for the hexose of benzoyl or 1,5 are ethanoyl, other is the five-carbon sugar of benzoyl.Purity can be directly used in next step reaction greater than 90%.
(2) with resulting 1; 6 is ethanoyl, other is the hexose of benzoyl or 1; 5 for ethanoyl, other is dissolved in the dimethyl formamide solution for the five-carbon sugar of benzoyl; the volatile salt that adds equimolar porphyrize is after reacting 20 hours under 30 ℃; ordinary method processing reaction liquid; crude product is refining with silica gel column chromatography; obtain that the inventive method prepares 1 for hydroxyl, 6 for ethanoyl, other for the hexose of benzoyl or 1 for hydroxyl, 5 are ethanoyl, other is the five-carbon sugar of benzoyl, productive rate is at 70-89%.
2. as claimed in claim 1 a kind of with 1; 6 is ethanoyl; other is the hexose or 1 of benzoyl; 5 is ethanoyl; other is that 1 of intermediate preparation is hydroxyl for the five-carbon sugar of benzoyl; 6 is ethanoyl; other is that the hexose of benzoyl or 1 are hydroxyl; 5 is ethanoyl; other is the synthetic method of the five-carbon sugar of benzoyl; it is characterized in that described 6 are trityl; other is trityl for the hexose of benzoyl is 6; other is the glucose of benzoyl; 6 is trityl; other is the semi-lactosi of benzoyl, and 6 is trityl; other is the seminose of benzoyl.5 for trityl, other for the five-carbon sugar of benzoyl be 5 be trityl, other is the wood sugar of benzoyl; 5 is trityl, other is the pectinose of benzoyl, and 5 is trityl, other is the lyxose of benzoyl.
3. as claimed in claim 1 a kind of with 1; 6 is ethanoyl; other is the hexose or 1 of benzoyl; 5 is ethanoyl; other is that 1 of intermediate preparation is hydroxyl for the five-carbon sugar of benzoyl; 6 is ethanoyl; other is that the hexose of benzoyl or 1 are hydroxyl; 5 is ethanoyl; other is the synthetic method of the five-carbon sugar of benzoyl; it is characterized in that described 1; 6 is ethanoyl; other for the hexose of benzoyl be 1; 6 is ethanoyl; other is the glucose of benzoyl; 1; 6 is ethanoyl; other is the semi-lactosi of benzoyl; 1,6 is ethanoyl; other is the seminose of benzoyl.Described 1,5 for ethanoyl, other for the pentasaccharides of benzoyl be 1,5 be ethanoyl, other is the wood sugar of benzoyl; 1; 5 is ethanoyl, other is the pectinose of benzoyl, and 1,5 is ethanoyl, other is the lyxose of benzoyl.
4. as claimed in claim 1 a kind of with 1; 6 is ethanoyl; other is the hexose or 1 of benzoyl; 5 is ethanoyl; other is that 1 of intermediate preparation is hydroxyl for the five-carbon sugar of benzoyl; 6 is ethanoyl; other is that the hexose of benzoyl or 1 are hydroxyl; 5 is ethanoyl; other is the synthetic method of the five-carbon sugar of benzoyl; it is characterized in that described 1 is hydroxyl; 6 is ethanoyl; other is hydroxyl for the hexose of benzoyl is 1; 6 is ethanoyl; other is the glucose of benzoyl; 1 is hydroxyl; 6 is ethanoyl; other is the semi-lactosi of benzoyl, and 1 is hydroxyl; 6 is ethanoyl; other is the seminose of benzoyl.Described 1 for hydroxyl, 5 for ethanoyl, other for the five-carbon sugar of benzoyl be 1 for hydroxyl, 5 are ethanoyl, other is the wood sugar of benzoyl; 1 is hydroxyl, 5 for ethanoyl, other be the pectinose of benzoyl, and 1 is that hydroxyl, 5 are ethanoyl, other is the lyxose of benzoyl.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB991197550A CN1138783C (en) | 1999-09-30 | 1999-09-30 | Synthesis of 1-hydroxy, 6 or 5-acetyl monosaccharide benzoylate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB991197550A CN1138783C (en) | 1999-09-30 | 1999-09-30 | Synthesis of 1-hydroxy, 6 or 5-acetyl monosaccharide benzoylate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1290704A true CN1290704A (en) | 2001-04-11 |
| CN1138783C CN1138783C (en) | 2004-02-18 |
Family
ID=5281081
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB991197550A Expired - Fee Related CN1138783C (en) | 1999-09-30 | 1999-09-30 | Synthesis of 1-hydroxy, 6 or 5-acetyl monosaccharide benzoylate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1138783C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100432089C (en) * | 2005-05-13 | 2008-11-12 | 湖南中烟工业有限责任公司 | Process of synthesizing 2,3,4,6-tetraacyl pyrane glucose |
| CN108314696A (en) * | 2018-03-16 | 2018-07-24 | 上药康丽（常州）药业有限公司 | 2- hydroxyls -1,3, the utilization method of tri--O- benzoyls-α of 5--D-RIBOSE crystalline mother solution |
-
1999
- 1999-09-30 CN CNB991197550A patent/CN1138783C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100432089C (en) * | 2005-05-13 | 2008-11-12 | 湖南中烟工业有限责任公司 | Process of synthesizing 2,3,4,6-tetraacyl pyrane glucose |
| CN108314696A (en) * | 2018-03-16 | 2018-07-24 | 上药康丽（常州）药业有限公司 | 2- hydroxyls -1,3, the utilization method of tri--O- benzoyls-α of 5--D-RIBOSE crystalline mother solution |
| CN108314696B (en) * | 2018-03-16 | 2021-07-13 | 上药康丽（常州）药业有限公司 | Utilization method of 2-hydroxy-1, 3, 5-tri-O-benzoyl-alpha-D-ribofuranose crystallization mother liquor |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1138783C (en) | 2004-02-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Jansson et al. | Structure of the extracellular polysaccharide from Xanthomonas campestris | |
| Li et al. | Low-molecular-weight fucosylated glycosaminoglycan and its oligosaccharides from sea cucumber as novel anticoagulants: A review | |
| EP2837635B1 (en) | New intermediates for the preparation of heparin pentasaccharide and preparation methods thereof | |
| KR101206288B1 (en) | Ascorbic acid derivates, their preparation methods, intermediates and uses in cosmetics | |
| CN103665057B (en) | A kind of synthetic method of Bromotetraacetylgluc,se | |
| CN106432538A (en) | Method for preparing chitin oligosaccharides, chitooligosaccharides and chitosan oligosaccharides | |
| Wang et al. | Assembly of a β-(1→ 3)-glucan laminarihexaose on ionic liquid support | |
| EP0165134B1 (en) | Oligosaccharides, their preparation by synthesis and their biological use | |
| CN1138783C (en) | Synthesis of 1-hydroxy, 6 or 5-acetyl monosaccharide benzoylate | |
| CN112538099B (en) | Preparation method and application of 1-thioglucose and glucose 1-thiol protected by total acyl | |
| JP2587268B2 (en) | Method for producing low-viscosity hyaluronic acid or salt thereof | |
| CN1233664C (en) | Method for preparing water soluble chitosan | |
| CN1290705A (en) | Synthesizing method of 1-hydroxy monosaccharide benzoylate | |
| CN1054607C (en) | Zone selection using saccharous ortho-ester as key intemediate and method for stereospecific synthesis of oligose | |
| JP2000351790A (en) | Production of fucose-containing oligosaccharide, or its composition and fucose-containing oligosaccharide or its composition | |
| Hassid et al. | Isolation and Structure of an Enzymatically Synthesized Crystalline Disaccharide D-Glucosido-D-ketoxyloside | |
| CN1680571A (en) | Preparation of maltotriose | |
| CN1072677C (en) | Method for synthesizing disaccharide and trisaccharide ortho-esters | |
| JP3939592B2 (en) | Method for producing oligosaccharide (salt) | |
| CN101235058A (en) | Method for synthesizing N-acetaminoglucose | |
| JP3650409B2 (en) | Process for producing low molecular weight branched β-1,3-glucan and branched laminary oligosaccharide | |
| JP3713065B2 (en) | Disaccharide monomer, oligomer thereof and method for producing oligomer | |
| Ning et al. | A simple approach to 3, 6-branched galacto-oligosaccharides and its application to the syntheses of a tetrasaccharide and a hexasaccharide related to the arabinogalactans (AGs) | |
| CN1129600C (en) | Synthesis of lentinan core fragment trisaccharide, tetrasaccharide, hexasaccharide and heptasaccharide | |
| CN114736316B (en) | Green preparation method and application of acetylated hyaluronate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |