CN1281900A

CN1281900A - Extraction method of abamectin

Info

Publication number: CN1281900A
Application number: CN 00121469
Authority: CN
Inventors: 高东卫
Original assignee: Individual
Current assignee: Individual
Priority date: 2000-07-25
Filing date: 2000-07-25
Publication date: 2001-01-31

Abstract

The method for extracting abamectin is implemented by means of improvement on the basis of existent extraction method of abamectin, and its main improvement is characterized by adding drying treatment procedure of wet mycelium after the filtering procedure of fermenting liquor and adopting rotary flash drier to make wet mycelium whose moisture content is 60-90% into dry mycelium whose moisture content is 5-35%, in which contact temp. of material and hot air is 50-100 deg.C. Said invention can greatly reduce production cost, and its process is simple and operation is convenient.

Description

Extraction method of abamectin

The present invention relates to a kind of extraction method of abamectin.

Avrmectin (Avermectin) is a kind of antiinsect antibiotic, belongs to public nuisance-free agricultural chemicals.At present, the extraction process step of Avrmectin is as follows: the preservation of A, bacterial classification and seed preparation: Avrmectin produces bacterium and grows on the YMS inclined-plane, spore is placed the aqueous glycerin solution of 20% (w/w), in 30 ℃ of preservations again; Seed culture is carried out on two kinds of substratum usually, inoculates in the fermention medium after 28 ℃ are cultivated 1-2 days, and inoculum size is 3-5%.B, fermentation: about 10 days of fermentation period.C, filtering fermentation liquor: adopt Plate Filtration.D, extraction, reconcentration are to paste: directly with the Avrmectin in ethanol or the acetone leaching mycelia filter cake, and the leaching liquid macroporous resin adsorption, getting off or directly be concentrated into desorbs such as acetone more to a certain degree extracts, and then reconcentration is to paste.F, dissolving and periodic crystallisation and finished product: dissolve periodic crystallisation and must finished product with the ethanol equal solvent.The shortcoming of said extracted method is the extraction cost height, and general extraction cost is about 2500 yuan of/kilogram Avrmectins.Having restricted the big area of preparation on agricultural that this harmless boilogical agricultural chemicals is mixed with so largely promotes the use of.Making a low multiple use of its pair product in addition, difficulty reaches national requirements for environmental protection.

The purpose of this invention is to provide and a kind ofly can reduce extraction cost greatly, technology is simple, the extraction method of abamectin of being convenient to operate.

The objective of the invention is to realize by the following technical solutions:

It is to improve to form on the basis of existing extraction method of abamectin, its main improvement is to increase wet mycelium drying treatment operation after fermentation liquid filters operation, the employing Rotatingandflashstreamingdrier is that the wet mycelium drying of 60-90% is the dried mycelium of 5-35% for water capacity with water capacity, and wherein material is 50-100 ℃ with the temperature that contacts of warm air.

After drying is handled,, greatly reduce the consumption of later stage leaching, greatly reduce production cost with ethanol or acetone because moisture reduces in the wet mycelium.In addition, because dried mycelia particle tiny (about 20-50 order), protein denaturation has increased later stage leaching surface-area, and leaching is abundant, thorough, and foreign matter content is low.

Adopt Rotatingandflashstreamingdrier to carry out drying treatment, can guarantee that effective constituent is not destroyed in treating processes, its reason is that rotary flash distillation has time of drying short (about 3-5 second), and material contacts the characteristics of temperature low (50-100 ℃).

Processing step of the present invention is as follows:

A kind of extraction method of abamectin, its processing step is as follows: preservation of (1) bacterial classification and seed preparation; (2) fermentation; (3) fermentation liquor pretreatment: promptly after fermentation ends, the fermented liquid open steam is heated to 60-95 ℃; (4) filtering fermentation liquor; (5) wet mycelium drying treatment: soon the wet mycelium behind the filtering fermentation liquor carries out drying treatment by Rotatingandflashstreamingdrier, makes it become the dried mycelium that water capacity is 5-35%, and wherein, material is 50-100 ℃ with the temperature that contacts of warm air; (6) dried mycelium is pulverized: dried mycelium powder is broken to the 50-150 order; (7) lixiviate, filtration; (8) extraction, reconcentration become paste; (9) dissolving and periodic crystallisation and finished product.

Adopt the fermentation liquor pretreatment operation that following benefit is arranged: (1) fermented liquid has passed through inactivation treatment, provides to produce for bacterial classification is stolen to ensure.(2) considerable part foreign protein sex change, dehydration, for later stage leaching drops into and quality all has obvious benefit, the carrying out smoothly of favourable big production.

The benefit that will pulverize operation with dried mycelium be: (1) Avrmectin belongs to intracellular product, and mechanical disintegration again after the dry dehydration helps physically destroying cell walls, helps the later stage leaching and discharges; (2) pulverize the back and increase surface-area, helping the later stage fully leaches, and improves yield; (3) increase the flowability of lixiviate mixed solution, prevented sedimentation.

Advantage of the present invention is to greatly reduce extraction cost, extracts the per kilogram Avrmectin 500-1000 unit that can reduce cost.Ethanol that adopts Rotatingandflashstreamingdrier to handle to use behind the wet mycelia or amounts of acetone are about 1/3 of former technology institute consumptions.And consequent vat liquor water-soluble impurity is few, and quality is good, is the realization of later stage direct crystallization operation, provides technical guarantee such as technologies such as absorption, extractions in the middle of exempting.Simultaneously it also to have technology simple, the characteristics of being convenient to operate.

Embodiments of the invention are as follows:

The extraction process of Avrmectin, its processing step and processing condition (Therefore, omited) all same as the prior art just increase three process.Promptly behind fermentation procedure, increase the fermentation liquor pretreatment operation, promptly after fermentation ends, the fermented liquid open steam is heated to 60-95 ℃.The optimum control temperature is 80-85 ℃.

After the filtering fermentation liquor operation, increase wet mycelium drying treatment operation, the wet mycelium that with water capacity is 60-90% is the dried mycelium of 5-35% for water capacity through the Rotatingandflashstreamingdrier drying, material is 50-100 ℃ with the temperature that contacts of warm air, and optimum temps is 60-80 ℃.

After wet mycelium drying treatment operation, increase dried mycelium and pulverize operation, dried mycelium powder is broken to the 50-150 order.

Claims

1, a kind of extraction method of abamectin, its processing step is as follows: the preservation of A, bacterial classification and seed preparation; B, fermentation; C, filtering fermentation liquor; D, lixiviate, filtration; E, extraction, reconcentration become paste; F, dissolving and periodic crystallisation and finished product, it is characterized in that C increases wet mycelium drying treatment operation after the step, soon the wet mycelium behind the filtering fermentation liquor carries out drying treatment by Rotatingandflashstreamingdrier, make it become the dried mycelium that water capacity is 5-35%, wherein, material is 50-100 ℃ with the temperature that contacts of warm air.

2, according to the described extraction method of abamectin of claim 1, it is characterized in that increasing the fermentation liquor pretreatment operation in B step back, promptly after fermentation ends, the fermented liquid open steam is heated to 60-95 ℃.

3, according to claim 1 or 2 described extraction method of abamectin, it is characterized in that after wet mycelium drying treatment operation, increasing dried mycelium and pulverize operation, dried mycelium powder is broken to the 50-150 order.