CN1280192A - Cell dyeing liquid - Google Patents
Cell dyeing liquid Download PDFInfo
- Publication number
- CN1280192A CN1280192A CN 00109660 CN00109660A CN1280192A CN 1280192 A CN1280192 A CN 1280192A CN 00109660 CN00109660 CN 00109660 CN 00109660 A CN00109660 A CN 00109660A CN 1280192 A CN1280192 A CN 1280192A
- Authority
- CN
- China
- Prior art keywords
- liquid
- distilled water
- cell dyeing
- proportioning
- formic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000007788 liquid Substances 0.000 title claims abstract description 45
- 238000004043 dyeing Methods 0.000 title claims abstract description 14
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims abstract description 16
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims abstract description 8
- 235000019253 formic acid Nutrition 0.000 claims abstract description 8
- 229920000159 gelatin Polymers 0.000 claims abstract description 8
- 235000019322 gelatine Nutrition 0.000 claims abstract description 8
- 229910001961 silver nitrate Inorganic materials 0.000 claims abstract description 8
- 239000012153 distilled water Substances 0.000 claims description 14
- 108010010803 Gelatin Proteins 0.000 claims description 7
- 239000008273 gelatin Substances 0.000 claims description 7
- 235000011852 gelatine desserts Nutrition 0.000 claims description 7
- 239000001828 Gelatine Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 description 6
- 239000012530 fluid Substances 0.000 description 3
- 239000002932 luster Substances 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Cell dyeing liquid consists of two parts, including liquid A being water solution of silver nitrate and liquid B comprising gelatine, formic acid and water. It has features of homogeneous dyeing, clear background, stable color, easy use, etc.
Description
The present invention relates to a kind of biological reagent, particularly a kind of cell dyeing liquid.
There are long, the deficiencies such as Color is poor, color and luster instability of dyeing time in cell dyeing liquid of the prior art.
The present invention has overcome the deficiencies in the prior art, and a kind of even dyeing, background is clear, color and luster is stable cell dyeing liquid are provided.
Staining fluid of the present invention is made up of A liquid and B liquid two portions, and the moiety of A liquid and proportioning thereof are: Silver Nitrate (analytical pure) 30-60g, distilled water 100ml.The optimum ratio of A liquid is: Silver Nitrate (analytical pure) 50g, distilled water 100ml.The compound method of A liquid is: in proportion Silver Nitrate is joined in the distilled water, fully stirs, until dissolving fully, and in the brown bottle of packing in 2-8 ℃ of preservations.
The moiety of B liquid and proportioning thereof are: gelatin 1-3g, pure formic acid 200-500 microlitres, distilled water 100ml.The optimum ratio of B liquid is: gelatin 2g, formic acid 250 microlitres, distilled water 100ml.The compound method of B liquid is: formic acid is joined in the distilled water, and mixing adds gelatin again, mixes, and puts into 37 ℃ then and cultivates or room temperature dissolving extremely fully, promptly gets B liquid of the present invention.B liquid is preserved down in 2-8 ℃.
During cell dyeing, the volume ratio of A liquid and B liquid is: 1-3: 1.
In the preparation process of staining fluid of the present invention, employed vessel and other apparatus etc. all need sterilization to handle, and all operations all should meet aseptic technique, and method for disinfection and sterilization and aseptic technique method all belong to the known technology of this area.
Cell dyeing liquid of the present invention compared with prior art has following advantage:
(1) the sample Color is good, even dyeing, and background is clear;
(2) it is stable to dye good sample sheet color and luster with this staining fluid, does not fade in 1-2 months under the drying at room temperature condition.
Further specify the present invention below in conjunction with embodiment:
Example 1
As stated above, be formulated as follows the A liquid and the B liquid of proportioning:
A liquid: Silver Nitrate (analytical pure) 50g, distilled water 100ml.
B liquid: gelatin 2g, formic acid 250 microlitres, distilled water 100ml.
A liquid and B liquid use by 1.5: 1 volume ratios.
Example 2
As stated above, be formulated as follows the A liquid and the B liquid of proportioning:
A liquid: Silver Nitrate (analytical pure) 50g, distilled water 100ml.
B liquid: gelatin 2g, formic acid 340 microlitres, distilled water 100ml.
A liquid and B liquid use by 2: 1 volume ratios.
Claims (2)
1. cell dyeing liquid, it is made up of A liquid and B liquid two portions, and it is characterized in that: the moiety of A liquid and proportioning thereof are: Silver Nitrate (analytical pure) 30-60g, distilled water 100ml; The moiety of B liquid and proportioning thereof are: gelatin 1-3g, pure formic acid 200-500 microlitres, distilled water 100ml;
A liquid and B liquid use by volume at 1-3: 1;
2. cell dyeing liquid as claimed in claim 1 is characterized in that:
The moiety of A liquid and proportioning thereof are: Silver Nitrate (analytical pure) 50g, distilled water 100ml;
The moiety of B liquid and proportioning thereof are: gelatin 2g, pure formic acid 250 microlitres, distilled water 100ml;
A liquid and B liquid use by volume at 1-3: 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00109660 CN1280192A (en) | 2000-06-21 | 2000-06-21 | Cell dyeing liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00109660 CN1280192A (en) | 2000-06-21 | 2000-06-21 | Cell dyeing liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1280192A true CN1280192A (en) | 2001-01-17 |
Family
ID=4579771
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00109660 Pending CN1280192A (en) | 2000-06-21 | 2000-06-21 | Cell dyeing liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1280192A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109963468A (en) * | 2016-10-05 | 2019-07-02 | 先正达参股股份有限公司 | The method for saving ribonucleic acid bioactivity |
-
2000
- 2000-06-21 CN CN 00109660 patent/CN1280192A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109963468A (en) * | 2016-10-05 | 2019-07-02 | 先正达参股股份有限公司 | The method for saving ribonucleic acid bioactivity |
| CN109963468B (en) * | 2016-10-05 | 2022-10-04 | 先正达参股股份有限公司 | Method for preserving ribonucleic acid biological activity |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| ASS | Succession or assignment of patent right |
Owner name: BEIJING JIANERKANG MEDICINE EQUIPMENT CO., LTD. Free format text: FORMER OWNER: ZHANG GUOQING Effective date: 20010912 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20010912 Address after: 100088 Building No. 7, West Lane, Zhichun Road, Haidian District, Beijing, Luozhuang Applicant after: Beijing jianerkang Medical Equipment Co., Ltd. Address before: 100080 Beijing city Haidian District Xiyuan No. 15 Haidian Sports Institute in the playground Applicant before: Zhang Guoqing |
|
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |