CN1279111A - Nutritive bioenzyme liquor for sobering up and its preparing process - Google Patents
Nutritive bioenzyme liquor for sobering up and its preparing process Download PDFInfo
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- CN1279111A CN1279111A CN99115433A CN99115433A CN1279111A CN 1279111 A CN1279111 A CN 1279111A CN 99115433 A CN99115433 A CN 99115433A CN 99115433 A CN99115433 A CN 99115433A CN 1279111 A CN1279111 A CN 1279111A
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- sobering
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
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Abstract
A sobering-up nutritive bioenzyme liquor is prepared from raw liquor, sobering-up factor, alcohol dehydrogenase, acetaldehyde dehydrogenase, fructose, lactamic acid, zinc-enriching element, edible salt and water. Said raw liquor is obtained by culturing the gene reambined bay bacillus in Chinese-medicinal culture liquid. Said sobering-up factor is prepared from lotus root, etc. Its advantages include no poison, high safety and high sobering-up effect. It can inerease drinking capacity by 1-4 times.
Description
The present invention is a kind of nutritive bioenzyme liquor for sobering up and preparation method thereof, belong to plant extract, probiotic bacteria metabolite and enzyme be raw material drink the preparation technical field.
As everyone knows, ethanol endangers seriously the human liver, not only diminishes human health, even constitute social unstable factor, might form one of 21st century public hazards according to estimates.And excessive drinking not only damages liver, and to the human body cranial nerve, heart and pancreas are all extremely harmful.This is that back ethanol spreads rapidly in the small intestinal in vivo and is absorbed because human body is drunk.Ethanol absorbs without stomach but gastric mucosa contains ethanol dehydrogenase (ADH), and some falls in the gastric metabolism ethanol before little intestinal absorption.Ethanol hepatocyte metabolic pathway in vivo is by microsome oxidation of ethanol enzyme system (MEOS) on the ethanol dehydrogenase in the cell cytoplasm liquid (ADH), the endoplasmic reticulum and the very big acetaldehyde of catalase three accelerating oxidation toxigenicity on the peroxidating corpusculum, therefore acetaldehyde is accumulated and Blushing occurred in the acetaldehyde metabolism delay person blood, and heart beating overruns that tachypnea is felt sick and the acute alcoholism state of dead drunk ferment wine such as headache.At present only to relieve the effect of alcohol soup decoct or organize compound recipe certainly of classical prescription medicine such as Flos puerariae lobatae, as antialcoholism capsule, the research of medicinal Chinese prescriptions such as the clever electuary of sobering up relates to all that raw material is most to be Chinese medicines such as Radix Puerariae, long-pending plank.Yet practice shows that these curative effect of medicine are still very indefinite, and especially the chemical constitution of its active ingredient is still indeterminate so can't carry out effective Quality Control to it.
The object of the present invention is to provide a kind of non-toxic and safe, nutritive bioenzyme liquor for sobering up and preparation method thereof efficiently.
Technical solution of the present invention is, a kind of nutritive bioenzyme liquor for sobering up and preparation method thereof is provided, described nutritive bioenzyme liquor for sobering up is by stock solution, sobering-up factor, ethanol dehydrogenase and aldehyde dehydrogenase, fructose, alanine, collect rich zinc element, raw material such as Sal and water is pressed 18-20: 5-10: 3.5-4: 3-10: 2-3: 0.2-0.4: 1-3: 49-67 successively: the mixed preparation of weight ratio compatibility, wherein:
A, described stock solution are to be cultivated 48 hours as nutrient medium at the plant winter fiber crops seed core, Fructus Schisandrae Chinensis, Fructus Lycii, Fructus Crataegi, skim Fructus Hordei Germinatus, green tea and the water that contain with dietotherapeutic by a kind of bacillus subtilis of gene recombinaton, temperature 35-45 ℃, the gained culture fluid after filtration, sterilization treatment preparation.
B, described sobering-up factor be with raw materials such as Rhizoma Nelumbinis, winter fiber crops fruit seeds successively by 30-50: 1-3 weight ratio compatibility mixed and pulverize the back with methanol, acetone, ethanol, ethyl acetate and hexane polar solvent routinely mixed extract, through 2-4 the filtration in 3-5 hour that refluxes respectively, to filter again the thing vacuum concentration, after concentrating concentrate is carried out fractional distillation, the fractional condensation thing carries out column chromatography with Sephadex, LH-20 and prepares, and mainly becomes to be tannin and Saponin.
C, the extraction of described ethanol dehydrogenase and aldehyde dehydrogenase is with Semen phaseoli radiati powder, Semen Raphani, Fructus Cucumidis sativi, pears and deionized water be raw material successively by 1-3: 1-3: 1-3: 1-3: 10-30 weight ratio compatibility is mixed pulverizes 10-20 minute pulping, centrifugal treating, add in the layer liquid thereon then sulphuric acid amine when making its concentration reach 20-30% secondary centrifuging handled 3-7 minute, collection gained precipitation, is 6.0 with the phosphate buffer of 0.1mol/L at pH value with the gained precipitate, temperature is to keep 5min to hatch under 37 ℃ of conditions, adsorb through DEAE-Tuyopearl post layer again, eluting, distilling under reduced pressure then prepares through gel filtration again.Because in the nutritive bioenzyme liquor for sobering up of the present invention as above-mentionedly used a large amount of bringing down a fever, removing food stagnancy and detoxifcation natural plant food, as Rhizoma Nelumbinis, has the stopping leak of helping digestion according to the supplement to the Herbal record, relieving restlessness, deinebriating effect, Fructus Crataegi, has removing food stagnancy according to Compendium of Material Medica and " classification medical herbs " record, in disappearing every the property of medicine of gas, and Semen phaseoli radiati powder has Xie Zhure according to " daily book on Chinese herbal medicine " record, QI invigorating, separate all malicious effects of food and drink, Semen Raphani, wide mouthful of the energy therapeutic method to keep the adverse QI flowing downwards, it is stagnant with the intestines and stomach to eliminate expansion, and Fructus Cucumidis sativi is carried the heat that also can remove in the heart according to " the southern regions of the Yunnan Province book on Chinese herbal medicine ", dredging water passages, separate excessive thirst, pears are the cool heart of lung moistening then, more can the antiinflammatory pathogenic fire reducing, separate sore alcoholic intoxication etc.High activity ethanol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) can promote acetaldehyde to be decomposed into nontoxic acetic acid and water rapidly.In addition, as the bacillus subtilis of the primary raw material said gene of stock solution reorganization in Chinese medicine nutritional solutions such as containing winter fiber crops seed core, Fructus Schisandrae Chinensis, Fructus Lycii, Fructus Crataegi is cultivated through biofermentation, produced a large amount of VitC, several amino acids, trophic factors such as tannin, Saponin.
So in sum, nutritive bioenzyme liquor for sobering up of the present invention have really bring down a fever, removing food stagnancy, rapid decomposing alcohol, efficiently suppress the absorption of ethanol and quicken metabolism, because its materials are edible plant mostly, therefore it is nontoxic to take the outstanding tool of this nutritive bioenzyme liquor for sobering up, the characteristics of safety.More good to eat when nutritive bioenzyme liquor for sobering up of the present invention is drunk, richer taste and favourable storage also can add an amount of thickening agent such as agar in above-mentioned finished fluid, an amount of essence such as strawberry essence or cream flavour and antiseptic such as potassium sorbate or sodium benzoate.Concrete prescription and preparation method be by raw materials such as stock solution, sobering-up factor, ethanol dehydrogenase and aldehyde dehydrogenase, fructose, alanine, the rich zinc element of collection, Sal, water, agar, edible essence and potassium sorbate successively by 18-20: 5-10: 3.5-4: 3-10: 2-3: 0.2-0.4: 1-3: 48-67: 0.03-0.05: 0.5-1.5: 0.003-0.005 weight ratio compatibility mixes and is prepared from.
Embodiment 1: get 200 kilograms of stock solutions, 80 kilograms of sobering-up factors, ethanol dehydrogenase and 35 kilograms of aldehyde dehydrogenase lyophilized preparation, 50 kilograms in fructose, 25 kilograms of alanine, 3 kilograms of rich zinc element of collection, 10 kilograms of Sal, 0.3 kilogram in agar, 1 kilogram of strawberry essence, 550 kilograms of 0.04 kilogram of potassium sorbate, sterile pure water evenly mixed refined biometric enzymolysis wine nutritional solution.200 kilograms of stock solutions wherein are to form for the bacillus subtilis culture fluid after under 45 ℃ of temperature conditions aseptic 30 minutes filters.80 kilograms of sobering-up factors wherein be with total amount 25% Hunan Rhizoma Nelumbinis, winter of 15% fiber crops fruit seed compatibility is mixed and pulverize the back with methanol, acetone, ethanol, ethyl acetate and hexane polar solvent routinely mixed extract, reflux respectively through three times and to filter in 4 hours, vacuum concentration filter thing, fractional distillation gained concentrate carries out column chromatography preparation with Sephadex, LH-20.Above-mentioned 35 kilograms of ethanol dehydrogenase and aldehyde dehydrogenase lyophilized preparation are that Semen phaseoli radiati powder, 8% Semen Raphani, 8% Fructus Cucumidis sativi, 8% pears with total amount 8% and 68% deionized water are poured in the blender and pulverized centrifugal treating about 15 minutes.Adding sulphuric acid amine in the layer liquid thereon then makes strength of fluid reach 25%, centrifugal treating is 5 minutes again, collection gained precipitation, is 6.0 with phosphate buffer 6 gram of 0.1mol/L at pH value with the gained precipitate, temperature is to keep 5min to hatch under 37 ℃ of conditions, again through the absorption of DEAE-Tuyopearl post layer, eluting, distilling under reduced pressure forms through gel filtration is refining again.
Embodiment 2: get stock solution 1800 grams, sobering-up factor 1000 grams, ethanol dehydrogenase and aldehyde dehydrogenase 400 grams, fructose 500 grams, alanine 200 grams collect rich zinc element 25 grams, Sal 200 grams, agar 5 grams, cream flavour 10 grams, sodium benzoate 3 grams and the 6082 gram blend of sterilization water evenly stir into the nutritive bioenzyme liquor for sobering up finished product.The preparation of raw materials such as stock solution wherein, sobering-up factor, ethanol dehydrogenase and aldehyde dehydrogenase and embodiment 1 are together.
Embodiment 3, get stock solution 6000 grams, sobering-up factor 3000 grams, ethanol dehydrogenase and aldehyde dehydrogenase 1200 grams, aspartoyl phenylalanine formicester 900 grams, fructose 2400 grams, alanine 750 grams, rich zinc 90 grams of collection, Sal 900 grams and sterilization water 6001 grams are mixed, evenly stir into the nutritive bioenzyme liquor for sobering up finished product, and the preparation of compositions such as stock solution wherein, sobering-up factor, ethanol dehydrogenase and aldehyde dehydrogenase and the foregoing description 1-2 are together.
Of the present invention this nutritive bioenzyme liquor for sobering up with above-mentioned preparation method making is on probation through manufacturing experimently, and is proved to be to reach designing requirement and expected effect fully.Getting nutritive bioenzyme liquor for sobering up kunming mice female with the 18-22 grammes per square metre, male half and half is experimental subject, and acute toxicity testing proves that this nutritional solution is nontoxic to body.Observe test group and matched group and [comprised the sterile saline group, Sobering up extraction oral liquid (Henan product) is pacified liquor-saturated celestial being (Japan produces) concentration of ethanol in serum, half an hour, gastric concentration of alcohol after 1 hour, interior and the L-AD of gastric mucosa, aldehyde dehydrogenase content, superoxide dismutase (SOD) concentration reaches with the white mice righting reflex loss in the erythrocyte, be relieving the effect of alcohol of index recovery time, anti-alcohol function, the result shows: the relieving the effect of alcohol of this nutritional solution group and above 3 groups of matched groups, the time showing of sobering up has significance to shorten, concentration of alcohol in the obvious decline blood, can improve gastric mucosa and hepatocyte ethanol dehydrogenase (ADH) and the active 5-10 of aldehyde dehydrogenase (ALDH) doubly, the active 2-4 of superoxide dismutase (SOD) doubly in the erythrocyte.Prompting: nutritive bioenzyme liquor for sobering up is alcohol metabolism in gastric has very strong decomposition ethanol and acceleration bodies, the body antioxidation is obviously increased, reach prevention acute and chronic alcoholism and hepatoprotective, antidotal alimentary health-care function, it is the clinical research crowd that the 24-30 of health year is not had the voluntary male of excessive drinking history, this nutritional solution can obviously improve ethanol in blood dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) concentration, reduces concentration of ethanol in serum, makes serum paraoxonase lipase A
2(PLA
2), slurry lipid peroxide (LPO) level reduces, the active effect that improves of erythrocyte superoxide dismutase (SOD), thus support zoopery result's announcement property.This nutritive bioenzyme liquor for sobering up of description of test on probation is the rapid decomposing alcohol of energy really, efficiently suppresses the absorption of ethanol and quickens its metabolism.After human body was taken, capacity for liquor increased 1-4 doubly and not drunk than usual, did not more have dead drunk symptom and took place and reach acute alcoholism due to the prevention insobriety.Also can remove halitosis after drinking, make the oral cavity have a kind of unusual delicate fragrance.In addition, take nutritive bioenzyme liquor for sobering up of the present invention, also can prevent due to the chronic alcoholism due to the insobriety as liver cirrhosis, infringement such as multiple neuropsychopathy and amnesia and health-care effect with nourishing the liver, the kidney invigorating, defying age and prophylaxis of tumours.
Claims (3)
1, a kind of nutritive bioenzyme liquor for sobering up and preparation method thereof, it is characterized in that described nutritive bioenzyme liquor for sobering up is by stock solution, sobering-up factor, ethanol dehydrogenase and aldehyde dehydrogenase, fructose, alanine, collect rich zinc element, raw material such as Sal and water is pressed 18-20: 5-10: 3.5-4: 3-10: 2-3: 0.2-0.4: 1-3: 49-67 successively: the mixed preparation of weight ratio compatibility, wherein:
A, described stock solution are to be cultivated 48 hours as nutrient medium containing winter fiber crops seed core, Fructus Schisandrae Chinensis, Fructus Lycii, Fructus Crataegi, skim Fructus Hordei Germinatus, green tea and water by a kind of bacillus subtilis of gene recombinaton, temperature 35-45 ℃, the gained culture fluid after filtration, sterilization treatment preparation.
B, described sobering-up factor be with raw materials such as Rhizoma Nelumbinis, winter fiber crops fruit seeds successively by 30-50: 1-3 weight ratio compatibility mixed and pulverize the back with methanol, acetone, ethanol, ethyl acetate and hexane polar solvent routinely mixed extract, through 2-4 the filtration in 3-5 hour that refluxes respectively, to filter again the thing vacuum concentration, after concentrating concentrate is carried out fractional distillation, the fractional condensation thing carries out column chromatography with Sephadex, LH-20 and prepares.
C, the extraction of described ethanol dehydrogenase and aldehyde dehydrogenase is with Semen phaseoli radiati powder, Semen Raphani, Fructus Cucumidis sativi, pears and deionized water be raw material successively by 1-3: 1-3: 1-3: 1-3: 10-30 weight ratio compatibility is mixed pulverizes 10-20 minute pulping, centrifugal treating, add in the layer liquid thereon then sulphuric acid amine when making its concentration reach 20-30% secondary centrifuging handled 3-7 minute, collection gained precipitation, is 6.0 with the phosphate buffer of 0.1mol/L at pH value with the gained precipitate, temperature is to keep 5min to hatch under 37 ℃ of conditions, adsorb through DEAE-Tuyopearl post layer again, eluting, distilling under reduced pressure then prepares through gel filtration again.
2, nutritive bioenzyme liquor for sobering up according to claim 1 and preparation method thereof, it is characterized in that described nutritive bioenzyme liquor for sobering up by raw materials such as stock solution, sobering-up factor, ethanol dehydrogenase and aldehyde dehydrogenase, fructose, alanine, the rich zinc element of collection, Sal, water, agar, edible essence and potassium sorbate successively by 18-20: 5-10: 3.5-4: 3-10: 2-3: 0.2-0.4: 1-3: 48-67: 0.03-0.05: 0.5-1.5: 0.003-0.005 weight ratio compatibility is mixed to be prepared from.
3, alcohol-decomposing beverage according to claim 2 is characterized in that described potassium sorbate also can be a sodium benzoate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99115433A CN1279111A (en) | 1999-06-23 | 1999-06-23 | Nutritive bioenzyme liquor for sobering up and its preparing process |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99115433A CN1279111A (en) | 1999-06-23 | 1999-06-23 | Nutritive bioenzyme liquor for sobering up and its preparing process |
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| Publication Number | Publication Date |
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| CN1279111A true CN1279111A (en) | 2001-01-10 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN99115433A Pending CN1279111A (en) | 1999-06-23 | 1999-06-23 | Nutritive bioenzyme liquor for sobering up and its preparing process |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102613366A (en) * | 2012-04-24 | 2012-08-01 | 哈尔滨工业大学 | Anti-alcoholic tea and preparation method thereof |
| CN102614500A (en) * | 2011-01-26 | 2012-08-01 | 权力敏 | Biological plant alcohol relieving and liver protecting capsule |
| CN101766716B (en) * | 2008-12-31 | 2012-08-29 | 克科 | Hangover relieving composition |
| CN101766637B (en) * | 2008-12-31 | 2012-08-29 | 克科 | Composite for disintoxicating and sobering |
| CN101766715B (en) * | 2008-12-31 | 2012-08-29 | 克科 | Composite for disintoxicating and sobering |
| CN101766672B (en) * | 2008-12-31 | 2012-10-10 | 克科 | Composition for anti-alcohol and sobering |
| CN101766635B (en) * | 2008-12-31 | 2012-10-10 | 克科 | Composite for disintoxicating and sobering |
| CN101766717B (en) * | 2008-12-31 | 2012-10-10 | 克科 | Composition for disintoxicating and sobering |
| CN101766636B (en) * | 2008-12-31 | 2012-11-28 | 克科 | Composite for disintoxicating and sobering |
| CN103143003A (en) * | 2013-03-22 | 2013-06-12 | 武汉工程大学 | Healthcare product for relieving hangover and protecting liver |
| CN103387915A (en) * | 2013-08-06 | 2013-11-13 | 郭品宏 | Yi nationality health-preserving healthcare wine |
| WO2015008101A1 (en) * | 2013-07-15 | 2015-01-22 | Schaumlöffel Rolf A | Composition of a drink for enhanced reduction of blood alcohol level |
| CN111642614A (en) * | 2020-06-04 | 2020-09-11 | 舜朵(德州)医药科技有限公司 | Anti-alcohol tabletted candy and preparation method thereof |
-
1999
- 1999-06-23 CN CN99115433A patent/CN1279111A/en active Pending
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101766635B (en) * | 2008-12-31 | 2012-10-10 | 克科 | Composite for disintoxicating and sobering |
| CN101766636B (en) * | 2008-12-31 | 2012-11-28 | 克科 | Composite for disintoxicating and sobering |
| CN101766716B (en) * | 2008-12-31 | 2012-08-29 | 克科 | Hangover relieving composition |
| CN101766637B (en) * | 2008-12-31 | 2012-08-29 | 克科 | Composite for disintoxicating and sobering |
| CN101766715B (en) * | 2008-12-31 | 2012-08-29 | 克科 | Composite for disintoxicating and sobering |
| CN101766672B (en) * | 2008-12-31 | 2012-10-10 | 克科 | Composition for anti-alcohol and sobering |
| CN101766717B (en) * | 2008-12-31 | 2012-10-10 | 克科 | Composition for disintoxicating and sobering |
| CN102614500A (en) * | 2011-01-26 | 2012-08-01 | 权力敏 | Biological plant alcohol relieving and liver protecting capsule |
| CN102613366A (en) * | 2012-04-24 | 2012-08-01 | 哈尔滨工业大学 | Anti-alcoholic tea and preparation method thereof |
| CN103143003A (en) * | 2013-03-22 | 2013-06-12 | 武汉工程大学 | Healthcare product for relieving hangover and protecting liver |
| CN103143003B (en) * | 2013-03-22 | 2014-03-26 | 武汉工程大学 | Healthcare product for relieving hangover and protecting liver |
| WO2015008101A1 (en) * | 2013-07-15 | 2015-01-22 | Schaumlöffel Rolf A | Composition of a drink for enhanced reduction of blood alcohol level |
| CN103387915A (en) * | 2013-08-06 | 2013-11-13 | 郭品宏 | Yi nationality health-preserving healthcare wine |
| CN103387915B (en) * | 2013-08-06 | 2016-01-13 | 郭品宏 | Man of a kind of Yi nationality health-care white spirit |
| CN111642614A (en) * | 2020-06-04 | 2020-09-11 | 舜朵(德州)医药科技有限公司 | Anti-alcohol tabletted candy and preparation method thereof |
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